MD01G1233000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013170
Physical Location & Seq
Reverse (-)
32123522 .. 32124774
1253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1233000.v1.1.491

Sequence Viewer

Length: 333 bp
ATGACGATACATACATGTTTTGGACCGAGTGTTTTTGGTATAACAATAACGGAACTGAGGGCTGCTCACACCTCGACTTATGACAAGAACCCGGAGGAACAAACTCGACCCAGCTTTGTTCCAGATGATGTGATCCCACCCCAACCGGACAAGTACTGGGCTCCACATCCCCACACGGGGGTGTTCGGGCCTGAAGCTGAACACACATCTTCCGCAGGCAGGGAGGACGGCTCCTCCACGGGCGATGGTGGGGAGGGATCCTCGGTGATGGAGCAGGAGGCGTGGTTTCGTCCTACCAGCATCGAGGACTTGGAGAAGCCTCACCTTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

12.02

Weight (kDa)

4.7

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3b PF22272 20 - 107 3.2e-35 Late embryogenesis abundant protein (LEA_3b subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03150 AT5G17165
fragaria_vesca FvH4_7g33540 FvH4_7g33540
malus_domestica MD01G1233000.v1.1 MD07G1305300.v1.1
prunus_persica Prupe.2G323400_v2.0.a1
pyrus_communis pycom01g24140
rosa_chinensis RchiOBHm_Chr1g0383761
rosa_laevigata RLG00000026098
rosa_multiflora Rmu_sc0012471.1_g000009
rosa_roxburghii Rroxscaffold_4G00277230
rosa_rugosa Rorug01G0442300
rosa_samantha Rh1AG467900 Rh1BG425500 Rh1CG438300 Rh1DG458300
rosa_wichuraiana Rw1G040450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 213
AclWI GGATC 3 cut(s) 127, 252, 265
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 4 cut(s) 176, 177, 178, 219
AflIII ACRYGT 1 cut(s) 14
AleI CACNNNNGTG 1 cut(s) 179
AluBI AGCT 2 cut(s) 114, 197
AluI AGCT 2 cut(s) 114, 197
AlwI GGATC 3 cut(s) 127, 252, 265
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 23, 188
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 277, 314
AvaII GGWCC 1 cut(s) 23
BamHI GGATCC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 49
BccI CCATC 2 cut(s) 239, 262
BceAI ACGGC 1 cut(s) 244
BcnI CCSGG 1 cut(s) 92
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 23, 188
BmiI GGNNCC 3 cut(s) 162, 232, 259
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 1 cut(s) 309
BmuI ACTGGG 1 cut(s) 166
BplI GAGNNNNNCTC 6 cut(s) 49, 81, 215, 247, 245, 277
BpuMI CCSGG 1 cut(s) 92
BsaJI CCNNGG 2 cut(s) 237, 261
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 269, 299
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 177, 178, 219
Bse1I ACTGG 1 cut(s) 161
BseDI CCNNGG 2 cut(s) 237, 261
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 4 cut(s) 176, 177, 178, 219
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 223
BseXI GCAGC 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 110
BshFI GGCC 1 cut(s) 190
BsiSI CCGG 2 cut(s) 92, 146
BslI CCNNNNNNNGG 4 cut(s) 176, 177, 178, 219
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 2 cut(s) 132, 257
BspACI CCGC 1 cut(s) 213
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 162, 232, 259
BspPI GGATC 3 cut(s) 127, 252, 265
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 237, 261
BssMI GATC 2 cut(s) 132, 257
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 2 cut(s) 135, 260
BstMBI GATC 2 cut(s) 132, 257
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 90
BstV1I GCAGC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 257
BstYI RGATCY 1 cut(s) 257
BsuRI GGCC 1 cut(s) 190
BtgI CCRYGG 1 cut(s) 237
BtgZI GCGATG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 23, 188
Csp6I GTAC 1 cut(s) 154
CviAII CATG 1 cut(s) 15
CviJI RGCY 7 cut(s) 62, 114, 161, 190, 197, 231, 319
CviKI_1 RGCY 7 cut(s) 62, 114, 161, 190, 197, 231, 319
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 1 cut(s) 56
DpnI GATC 2 cut(s) 134, 259
DpnII GATC 2 cut(s) 132, 257
Eco24I GRGCYC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 163
FaeI CATG 1 cut(s) 18
FaiI YATR 4 cut(s) 12, 16, 41, 81
FatI CATG 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 1 cut(s) 153
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 1 cut(s) 63
GluI GCNGC 1 cut(s) 63
GsaI CCCAGC 1 cut(s) 114
HaeIII GGCC 1 cut(s) 190
HapII CCGG 2 cut(s) 92, 146
Hin1II CATG 1 cut(s) 18
HpaII CCGG 2 cut(s) 92, 146
HphI GGTGA 2 cut(s) 277, 314
Hpy188III TCNNGA 1 cut(s) 122
HpyF3I CTNAG 1 cut(s) 56
Hsp92II CATG 1 cut(s) 18
Kzo9I GATC 2 cut(s) 132, 257
LmnI GCTCC 3 cut(s) 166, 236, 271
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 1 cut(s) 309
MalI GATC 2 cut(s) 134, 259
MboI GATC 2 cut(s) 132, 257
MboII GAAGA 1 cut(s) 201
MflI RGATCY 1 cut(s) 257
MhlI GDGCHC 1 cut(s) 163
MslI CAYNNNNRTG 1 cut(s) 179
MspI CCGG 2 cut(s) 92, 146
MspR9I CCNGG 1 cut(s) 92
NciI CCSGG 1 cut(s) 92
NdeII GATC 2 cut(s) 132, 257
NlaIII CATG 1 cut(s) 18
NlaIV GGNNCC 3 cut(s) 162, 232, 259
NspI RCATGY 1 cut(s) 18
OliI CACNNNNGTG 1 cut(s) 179
PciI ACATGT 1 cut(s) 14
PkrI GCNGC 1 cut(s) 64
PscI ACATGT 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 162, 232, 259
PspPI GGNCC 2 cut(s) 23, 188
PsuI RGATCY 1 cut(s) 257
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 179
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 2 cut(s) 132, 257
Sau96I GGNCC 2 cut(s) 23, 188
ScaI AGTACT 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 1 cut(s) 163
SetI ASST 4 cut(s) 74, 116, 199, 327
SfaNI GCATC 1 cut(s) 309
SinI GGWCC 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 179
SsiI CCGC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 90
TaqI TCGA 3 cut(s) 74, 106, 303
TaqII GACCGA 1 cut(s) 40
TatI WGTACW 1 cut(s) 153
TseI GCWGC 1 cut(s) 62
TspGWI ACGGA 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 23
XceI RCATGY 1 cut(s) 18
ZrmI AGTACT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.