MD07G1305300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013170
Physical Location & Seq
Reverse (-)
36229281 .. 36232986
3706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1305300.v1.1.491

Sequence Viewer

Length: 381 bp
ATGGCCGCCAATTCGAGCAGTCGAGGGATCGCGAGCTTGGGGAAGCGACTTATCAACGAGATCCGAACGCGCGATTCAGCTCAGCTCTCTGCTCTCACTCTCAGGAGGGCTGCTCACACCTCAATCTATGACAAGAACCCAGATGAGCAGATTCGACCTAGCGTTGTTCCAGATGATGTGATCCCGCCCCAACCTGACAAGTACTGGGCTCCAAACCCCCACACTGGGGTATTCGGGCCTGAAGCTGAACATACTTCTTCCGCAGGCAGGGAGGAAGGTTCCTCCCCAGTGAATGGTGGTGAGGGATCCTCGGTGCTGGAGCAGAAGGCTTGGTTCCGTCCTACCAGCATCGAGGACTTGGAGAAGCCTCACCTCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.7

Weight (kDa)

5.77

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3b PF22272 36 - 123 6.1e-36 Late embryogenesis abundant protein (LEA_3b subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03150 AT5G17165
fragaria_vesca FvH4_7g33540 FvH4_7g33540
malus_domestica MD01G1233000.v1.1 MD07G1305300.v1.1
prunus_persica Prupe.2G323400_v2.0.a1
pyrus_communis pycom01g24140
rosa_chinensis RchiOBHm_Chr1g0383761
rosa_laevigata RLG00000026098
rosa_multiflora Rmu_sc0012471.1_g000009
rosa_roxburghii Rroxscaffold_4G00277230
rosa_rugosa Rorug01G0442300
rosa_samantha Rh1AG467900 Rh1BG425500 Rh1CG438300 Rh1DG458300
rosa_wichuraiana Rw1G040450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 293
AccII CGCG 3 cut(s) 32, 70, 72
AciI CCGC 3 cut(s) 6, 185, 261
AclWI GGATC 5 cut(s) 35, 55, 175, 300, 313
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 5 cut(s) 224, 225, 226, 267, 293
AluBI AGCT 4 cut(s) 36, 80, 85, 245
AluI AGCT 4 cut(s) 36, 80, 85, 245
AlwI GGATC 5 cut(s) 35, 55, 175, 300, 313
AoxI GGCC 2 cut(s) 3, 236
ApeKI GCWGC 1 cut(s) 110
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 1 cut(s) 236
AsuHPI GGTGA 2 cut(s) 311, 362
BamHI GGATCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 211
BbvI GCAGC 1 cut(s) 97
BfaI CTAG 1 cut(s) 159
BisI GCNGC 2 cut(s) 6, 111
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 2 cut(s) 7, 112
BmcAI AGTACT 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 4 cut(s) 210, 280, 307, 335
BmrI ACTGGG 3 cut(s) 214, 234, 281
BmsI GCATC 1 cut(s) 357
BmuI ACTGGG 3 cut(s) 214, 234, 281
BplI GAGNNNNNCTC 4 cut(s) 97, 129, 293, 325
BpmI CTGGAG 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 5 cut(s) 224, 225, 226, 267, 293
Bse1I ACTGG 3 cut(s) 209, 229, 287
BseDI CCNNGG 1 cut(s) 309
BseLI CCNNNNNNNGG 5 cut(s) 224, 225, 226, 267, 293
BseMII CTCAG 2 cut(s) 95, 115
BseNI ACTGG 3 cut(s) 209, 229, 287
BseXI GCAGC 1 cut(s) 97
Bsh1236I CGCG 3 cut(s) 32, 70, 72
BshFI GGCC 2 cut(s) 5, 238
BslI CCNNNNNNNGG 5 cut(s) 224, 225, 226, 267, 293
BsnI GGCC 2 cut(s) 5, 238
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 4 cut(s) 27, 60, 180, 305
Bsp1720I GCTNAGC 1 cut(s) 81
Bsp68I TCGCGA 1 cut(s) 32
BspACI CCGC 3 cut(s) 6, 185, 261
BspANI GGCC 2 cut(s) 5, 238
BspCNI CTCAG 2 cut(s) 94, 114
BspFNI CGCG 3 cut(s) 32, 70, 72
BspLI GGNNCC 4 cut(s) 210, 280, 307, 335
BspPI GGATC 5 cut(s) 35, 55, 175, 300, 313
BsrI ACTGG 3 cut(s) 209, 229, 287
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 4 cut(s) 27, 60, 180, 305
BstC8I GCNNGC 2 cut(s) 34, 265
BstDEI CTNAG 2 cut(s) 81, 101
BstFNI CGCG 3 cut(s) 32, 70, 72
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 4 cut(s) 30, 63, 183, 308
BstMBI GATC 4 cut(s) 27, 60, 180, 305
BstUI CGCG 3 cut(s) 32, 70, 72
BstV1I GCAGC 1 cut(s) 97
BstX2I RGATCY 2 cut(s) 60, 305
BstYI RGATCY 2 cut(s) 60, 305
BsuRI GGCC 2 cut(s) 5, 238
BtsIMutI CAGTG 2 cut(s) 222, 294
BtuMI TCGCGA 1 cut(s) 32
Cac8I GCNNGC 2 cut(s) 34, 265
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 202
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 81, 101
DpnI GATC 4 cut(s) 29, 62, 182, 307
DpnII GATC 4 cut(s) 27, 60, 180, 305
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 211
FaiI YATR 2 cut(s) 129, 252
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 6, 111
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 2 cut(s) 6, 111
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 71
GluI GCNGC 2 cut(s) 6, 111
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 2 cut(s) 5, 238
HhaI GCGC 1 cut(s) 72
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HinfI GANTC 2 cut(s) 74, 151
HphI GGTGA 2 cut(s) 311, 362
Hpy188I TCNGA 1 cut(s) 65
Hpy188III TCNNGA 3 cut(s) 31, 103, 170
HpyAV CCTTC 2 cut(s) 269, 319
HpyF3I CTNAG 2 cut(s) 81, 101
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 4 cut(s) 27, 60, 180, 305
LmnI GCTCC 2 cut(s) 214, 319
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 1 cut(s) 357
MaeI CTAG 1 cut(s) 159
MalI GATC 4 cut(s) 29, 62, 182, 307
MboI GATC 4 cut(s) 27, 60, 180, 305
MboII GAAGA 1 cut(s) 249
MflI RGATCY 2 cut(s) 60, 305
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 1 cut(s) 10
MnlI CCTC 9 cut(s) 17, 99, 130, 265, 292, 295, 319, 346, 378
MvnI CGCG 3 cut(s) 32, 70, 72
NdeII GATC 4 cut(s) 27, 60, 180, 305
NlaIV GGNNCC 4 cut(s) 210, 280, 307, 335
NruI TCGCGA 1 cut(s) 32
PfeI GAWTC 2 cut(s) 74, 151
PflMI CCANNNNNTGG 1 cut(s) 293
PkrI GCNGC 2 cut(s) 7, 112
PspN4I GGNNCC 4 cut(s) 210, 280, 307, 335
PspPI GGNCC 1 cut(s) 236
PsuI RGATCY 2 cut(s) 60, 305
RruI TCGCGA 1 cut(s) 32
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SatI GCNGC 2 cut(s) 6, 111
Sau3AI GATC 4 cut(s) 27, 60, 180, 305
Sau96I GGNCC 1 cut(s) 236
ScaI AGTACT 1 cut(s) 203
SduI GDGCHC 1 cut(s) 211
SetI ASST 9 cut(s) 38, 82, 87, 122, 160, 196, 247, 280, 375
SfaNI GCATC 1 cut(s) 357
Sse9I AATT 1 cut(s) 10
SsiI CCGC 3 cut(s) 6, 185, 261
SspMI CTAG 1 cut(s) 159
TaqI TCGA 4 cut(s) 14, 22, 154, 351
TasI AATT 1 cut(s) 10
TatI WGTACW 1 cut(s) 201
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 74, 151
TscAI CASTG 2 cut(s) 229, 294
TseI GCWGC 1 cut(s) 110
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 2 cut(s) 229, 294
Van91I CCANNNNNTGG 1 cut(s) 293
XspI CTAG 1 cut(s) 159
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.