Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008066

Physical Location & Seq

Genomic Coordinates Reverse (-)

297679 .. 306509

Gene Structure 8831 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1004400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4908 bp

ATGGAATCAGCTGGTGGTGGTATAAGGGAGACATCTGTTGTAGTTGTGACGTTGGACCCTGGTGAAGTATATATAATTGCAAGTTTGTCTTCCAGGCTCGACACTCAGGTTATACATGTTGATCCCACAACCGGAGTGCTTCGCTACAATGCAAAGCCAGGATTTGATGTTTTCAAATCCGAAAAGGAAGCATTGGAATACGTTACGAACGGATCACCCTGGTTGTGTAAGAATACGACCTATGCACGTGCAATATTGGGTTATGCTGCTTTGGGCAGCATTGGTTTGCTTCTCGTGGCTACCAAGTTGACTGCTAGTGTTCCGAAATTGCCTGGTGGAGGGTGCATTTATACGGTGACTGAGAGCCAATGGATCAAGATTCAACTTCAGAATCCACAACCACAAGGGAAAGGAGAAGAAAAGAATGTTAATGAATTAACTGATCTTGACATTAATGGGAAGCACTACTTTTGTGACGCAAGGGATATCACTCGACCATTTCCTAGCCGTATGTGCTTGCGTGAGCCCGATGATGAATTTGTTTGGAACGCCTGGTTCTCGATGCCTTTCAAAAACATTGGGCTGCCGCAGCATTGTGTCACTCTTCTTCAGGGTTTTGCAGAGTGTCGAAGTTTTGGAACCTTAGGGAAGGTGAAAGGAATTGTTGCTCTCATAGCTCGTCGTAGCAGGCTGCATCCTGGTACTCGGTACTTGGCTAGGGGATTGAATTCATGTTTTAGCACAGGAAATGAAGTGGAGTGTGAGCAACTTGTATGGATCCCTAGAAGGGCTGGTCAAACTGTTCCTTTTAACACATACGTGTGGCGACGGGGTACAATTCCAATTTGGTGGGGTGCAGAGTTAAAGATTACTGCTGCAGAAGCAGAAATATACGTTTCGGATCGCGATCCTTATAAAGGAAGTTCGGAGTACTACCAGAGGTTGAGTAAGAGGTATGATGCACGAAATTTTGACGTAGCTGTTGGGGGGAGTCAGAATAGAAAGGCGTTTGTTCCAATTGTATGCATCAACTTGCTTAGGAGTGGAGAAGGAAAGTCAGAATGTATTTTAGTTCAACATTTTGAGAAATCTTTAAACTACATAAAGTCAACTGGAAAACTTCCTTATACTCGAATTCAATTGATAAATTATGACTGGCATGCCAGTATAAAGTTAAGAGGTGAACAGCAAACCATCGAAGGATTATGGAAACATCTAAAAGCACCCACTATTTCCATAGGCATTTCTGAAGGAGATTTTTTGCCTTCACGAGAAAGAATTAAGGACTGCAGAGGAGAAATTATCTACAATGATGACTTTGAAGGTGCTTTCTGCTTAAGATCACGTCAAAATGGTGTGATACGTTTGAACTGTGCTGATTCTTTGGATAGGACAAATGCTGCAAGTTATTTTGGCTCCCTCCAGGTTTTTGTGGAGCAGTGTAGGCGGCTTTGTATATCACTTGATAGTGATTTGGCATTTGGTTACCAGTCAATGAGTACTTATGGTGGCTATACAGCTCCTCTGCCACCAGGCTGGGAGAAGAGATCTGATGCAGTAACAGGGAAAACTTTTTATATTGATCACAATACTAGGACCACAACATGGACGCATCCGTGTCCTGATGAACCTTGGAAGAGATTTGATATGGCATTTGAGGAGTTCAAGAGGTCAACAATTTTATCACCAGTATCCCAGCTTGCTGATATTTTTCTGCTTGCGGGTGATATTCATGCAACACTTTATACCGGTTCGAAAGCTATGCATAGCCAAATCCTCAGCATCTTCAATGATGATGCAGGAAAATTCAAACAATTTTCTGCAGCACAGAATATGAAAATTACTTTGCAGAGGAGATATAAAAATGCAGTTGTAGACAGCTCTCGTCAAAAGCAACTTGAGATATTTCTTGGAATGAGACTATTCAAGCATCTTCCATCAGTTTCTTTTCACCCTCTTAATGTAGTCTCTCGACCATCTGGTTTCTTCCTTAAGCCAGTTGCTAACATGTTTCCAAGTTCCAAAGGTGGAGCCAGTCTTCTGAGTTTCAAGAGAAAGGATCTAGTCTGGGTTTGCCCACAGGCCGCAGATGTTGTTGAACTGTTTATCTATCTTGGTGAACCTTGCCATGTTTGTCAGCTTCTTCTCACAATATCCCATGGTGCAGATGATTCAACATATCCATCAACAGTTGATGTTAGGACAGGACGCTCTTTAGATGGGCTAAAACTTGTCTTGGAGGGTGCTTCAATACCTCAATGTATACATGGAACAAACCTCTTGATACCCTTAACAGGAGCAGTTAGTCCAGAGGATATGGCTATAACAGGAGCTGGTTCCCGTCTTCATGCTCAAGATACATCTACCCTTCCATTATTGTATGATTTTGAAGAACTGGAAGGAGAACTTGACTTCCTTACTCGTGTAGTTGCCCTTACATTTTATCCTGCTGTCTCTGGAAAGAGCCCTATCACTCTTGGTGAGATAGAAGTCCTTGGAGTTTCTCTTCCTTGGAGGGGTGTATTTACTAACGAAGGCCCTGGTGCAAGACTACCTGAACATGCTAAAAACCTTCAGAATGAAAACATTCATTTTTCCTCTGGGTCAAAAACCAATCCATTTTCTGGTGCTTCATTTACTGAAAATGTGCTGCCACCAGTGCAACCAAGTGCGTCTGCAAACAATTTAGTTGACCTATTGACTGGAGAGATGGTGCTTTCAGAACACATTGCTGCGCCAGTGATAGGAAATGTTGAGGACAAGGGAGGTGACCTTCTTGATTTCTTGGACCAAGCTATTGTTGAATATCATGATGCTGAAACTGATCATAAATCACATGATGGAAAACCTTCAGATAGCAGTTCCCAGCAGTACATTGATTGTTTGAAATCTGTTGCTGGGCCACATATGAAAAAGAAACTAAACTTCATGGAAGCCATGAAACTTGAAATTGAACGCCTCAGGCTGAACATTTCTGCTGCTGAAAGGGATAGAGCTTTGTTATCTATAGGAACTGATCCTGCTACTATAAACCCTAATGTTTTACTTGATGAACGATACATGGGAAGATTGTGCAGAGTTGCAAACTCCCTGGCACTGCTTGGACAAGCATCCTTGGAAGACAAAATTACATCTGCTGTTGGTCTTGAGACTACTGATGATAATGAGATAGATTTTTGGAATATAACTAGATTTGGTGACTGCTGTTATGGTGGCACGTGTGAGGTTCGTGCTGAAACTAATGCACCTACACGTCCTTCCTTTTTGGAATCAGGCGAAGTTTCCCCGTCTCTCTTGTTATGTTCCCAATGTGAAAGGAAAGTTTGTAAAGTTTGCTGTGCTGGTAGAGGGGCCCTTCTGGTTGCAGGCTATGGGTCAAGGGACGCCATGAGTTACAATGGTGTGGTAAATCAGGGGGGATCAGGCCATGGTTTCCAGGTTGACGTCTCTACAAATCGTACAGTAGTGCTGGATAGTGTTATTTGTAAAAGATGCTGCCATGATATTGTGCTTGATGCATTGATCTTGGACTACGTCAGGGTCTTGATAAGCATGAGGAGAAGTACACGTGCTGATTCTGCTGCCCATGAGGCCTTGAACCAGGTGATTGGATATTCAGTAAGGAATTGTCTTTATGAAAGGAAACAGTCTCCGGATAGAAAGAGAACTATTAAAGTTCTGCAGAAATTACTTGGCAGAGAGGAATCGCTAGCCGAGTTTCCATTTGCCAGCTTTTTACACTCGGTTGAAACGGGAGCAGATTCAGCACCATTCTTGTCATTGCTTGCTCCTCTTGAATCTGGATCACGCCATTCATACTGGAAAGCTCCTCCTAGTACCACCAGTGTTGAGTTCATTATTGTTCTTGGCACCCTTTCTGATGTTAGTGGGGTTGTTTTGCTTATTAGTCCATGTGGTTATTCTGAGGCTGATGCACCCACCGTGCAAATCTGGGCCAGCAATAAAATACACAAGGAAGAAAGGTCATGCATGGGAAAATGGGATTTGCAGTCCCAGATCACGGCTTCCTCAGAATATTATGGACATGAAAATTCGGTTAGAGAAAATGAAGTACCGAGGCATGTGAAGTTTGAATTTAGGAATCCAGTTCGATGCCGCATTATTTGGATAACATTACGCCTTCAGCGACCTGGTTCAAAATCTCTTAATTTGGACAACTTGAATCTATTGTCTCTCGATGAAAATCCATTTGCAGAAGTAACTCGGCGTTCTTCTTTTGGAGGAGAAGTTGAAAGAGACCCCTGTATTCATGCCAAAAGGATACTGGTAGTCGGAAGCCCGGTGAAAAAAGAGATGGAAGATACATCACAAAGCTACGATCAGATGAATCTGAAAGGCTGGCTGGAGAGAGGTCCACCACTGAATAGATTTAGGGTTCCCATTGAGGCCGAGAGGCTGTTGGACAATGATATTGTTTTGGAGCAATATCTTTCTCCTGCATCACCTTTGCTTGCTGGATTTCGTCTTGATGCTTTTGGTGCAATAAAGCCTCTGGTTACCCATTCACCCTCTTCAAATACACTGGTCTGGGATACGTCAGCCACACTAGTAGACGAGAGACACATCTCTCCAGCCGTTCTGTATATACAAGTATCTGTTGTCCAGGAACCCCAAAGCACGGTAACGATTGCAGAATATCGTTTGCCAGAGGCCAAGTCTGGAACAGCCATGTACTTTGATTTCCCTCGAGAGATACAAACCCGCACAATTATATTTAAACTTCTTGGAGATATTACAGCATTTGCCGATGACCCAACAGAGCAGGATGATGCCGGTTCCAGAGTGGTACCTGTGGCTGCGGGGTTGTCGTTGGCCAATAAAATTAAGTTGTATTACTACGCCGATCCATACGAACTTGGAAAATGGGCAAGCCTCTCGGCCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0031348 GO:0033612 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0060548 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Syja_N WW

Protein Analysis

1636

Amino Acids

181.22

Weight (kDa)

6.21

Isoelectric Point (pI)

43.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syja_N	PF02383	84 - 390	3.8e-40	SacI homology domain
WW	PF00397	509 - 539	7.5e-12	WW domain
SAC9_GBDL_1st	PF24790	653 - 838	2.6e-98	SAC9 first GBDL domain
SAC9_C8D	PF24791	1063 - 1173	2.6e-18	SAC9 C8D domain
SAC9_GBDL_2nd	PF24789	1234 - 1433	1.3e-100	SAC9 second GBDL domain
SAC9_C	PF24765	1461 - 1635	1.5e-89	SAC9 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0008066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59770 AT3G59770 AT3G59770 AT3G59770
fragaria_vesca	FvH4_1g00530 FvH4_1g00530
malus_domestica	MD02G1004400.v1.1 MD15G1148100.v1.1
prunus_persica	Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1
pyrus_communis	pycom02g00290 pycom15g13240
rosa_chinensis	RchiOBHm_Chr2g0085161
rosa_laevigata	RLG00000015661
rosa_multiflora	Rmu_sc0008647.1_g000009
rosa_roxburghii	Rroxscaffold_2G00155600
rosa_rugosa	Rorug01G0458000
rosa_samantha	Rh2AG006300 Rh2BG007100 Rh2CG007900 Rh2DG008100
rosa_wichuraiana	Rw2G000600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	915
AatII	GACGTC	1 cut(s)	3447
Acc65I	GGTACC	1 cut(s)	4807
AccB1I	GGYRCC	2 cut(s)	3869, 4807
AccB7I	CCANNNNNTGG	2 cut(s)	1605, 2624
AccI	GTMKAC	3 cut(s)	1875, 2263, 4572
AccII	CGCG	1 cut(s)	906
AccIII	TCCGGA	1 cut(s)	3652
AciI	CCGC	7 cut(s)	587, 1447, 1721, 2085, 4117, 4723, 4820
AcoI	YGGCCR	2 cut(s)	4833, 4899
AcsI	RAATTY	7 cut(s)	536, 727, 967, 1134, 1805, 4051, 4094
AcuI	CTGAAG	6 cut(s)	371, 593, 1269, 2558, 2835, 4127
AcvI	CACGTG	3 cut(s)	248, 3219, 3569
AcyI	GRCGYC	2 cut(s)	3384, 3444
AflII	CTTAAG	2 cut(s)	1336, 1991
AflIII	ACRYGT	6 cut(s)	115, 819, 2007, 3218, 3251, 3566
AgeI	ACCGGT	1 cut(s)	1748
AhlI	ACTAGT	1 cut(s)	4567
AjiI	CACGTC	2 cut(s)	1346, 3254
AleI	CACNNNNGTG	1 cut(s)	818
AloI	GAACNNNNNNTCC	2 cut(s)	2397, 2429
AlwNI	CAGNNNCTG	1 cut(s)	2333
Ama87I	CYCGRG	1 cut(s)	4707
Aor13HI	TCCGGA	1 cut(s)	3652
ApaI	GGGCCC	1 cut(s)	3355
ApoI	RAATTY	7 cut(s)	536, 727, 967, 1134, 1805, 4051, 4094
AseI	ATTAAT	1 cut(s)	453
AsiGI	ACCGGT	1 cut(s)	1748
Asp700I	GAANNNNTTC	2 cut(s)	2586, 2848
Asp718I	GGTACC	1 cut(s)	4807
AspLEI	GCGC	1 cut(s)	2737
AspS9I	GGNCC	9 cut(s)	55, 1596, 2537, 2788, 2900, 3351, 3352, 3954, 4373
AsuC2I	CCSGG	1 cut(s)	4301
AsuII	TTCGAA	1 cut(s)	1754
AsuNHI	GCTAGC	1 cut(s)	3709
AvaI	CYCGRG	1 cut(s)	4707
AvaII	GGWCC	4 cut(s)	55, 1596, 2788, 4373
AxyI	CCTNAGG	2 cut(s)	643, 2960
BaeGI	GKGCMC	1 cut(s)	3355
BalI	TGGCCA	1 cut(s)	4835
BamHI	GGATCC	1 cut(s)	777
BanI	GGYRCC	2 cut(s)	3869, 4807
BanII	GRGCYC	3 cut(s)	528, 2468, 3355
BauI	CACGAG	3 cut(s)	293, 1269, 2421
BbrPI	CACGTG	3 cut(s)	248, 3219, 3569
BbsI	GAAGAC	4 cut(s)	81, 2030, 2336, 3126
BbvCI	CCTCAGC	1 cut(s)	1778
BccI	CCATC	8 cut(s)	1202, 1945, 1984, 2191, 2213, 2704, 2834, 4309
BceAI	ACGGC	4 cut(s)	492, 4038, 4580, 4886
BcgI	CGANNNNNNTGC	3 cut(s)	1744, 1778, 4878
BciVI	GTATCC	3 cut(s)	1702, 4275, 4546
BclI	TGATCA	2 cut(s)	1582, 2824
BcnI	CCSGG	1 cut(s)	4301
BcuI	ACTAGT	1 cut(s)	4567
BfmI	CTRYAG	5 cut(s)	876, 1288, 1821, 3006, 3680
BfrI	CTTAAG	2 cut(s)	1336, 1991
BfuI	GTATCC	3 cut(s)	1702, 4275, 4546
BglI	GCCNNNNNGGC	1 cut(s)	3590
BglII	AGATCT	1 cut(s)	1547
BmcAI	AGTACT	2 cut(s)	932, 1501
Bme18I	GGWCC	4 cut(s)	55, 1596, 2788, 4373
BmeT110I	CYCGRG	1 cut(s)	4707
BmgBI	CACGTC	2 cut(s)	1346, 3254
BmgT120I	GGNCC	9 cut(s)	55, 1596, 2537, 2788, 2900, 3351, 3352, 3954, 4373
BmtI	GCTAGC	1 cut(s)	3713
BpiI	GAAGAC	4 cut(s)	81, 2030, 2336, 3126
BpmI	CTGGAG	4 cut(s)	1406, 2724, 4385, 4575
Bpu10I	CCTNAGC	2 cut(s)	1037, 1778
Bpu14I	TTCGAA	1 cut(s)	1754
BpuEI	CTTGAG	3 cut(s)	1919, 2337, 3167
BpuMI	CCSGG	1 cut(s)	4301
BsaAI	YACGTR	4 cut(s)	248, 820, 3219, 3569
BsaBI	GATNNNNATC	2 cut(s)	906, 4203
BsaHI	GRCGYC	2 cut(s)	3384, 3444
BsaI	GGTCTC	1 cut(s)	4251
BsaWI	WCCGGW	3 cut(s)	131, 1748, 3652
BsaXI	ACNNNNNCTCC	4 cut(s)	2697, 2727, 4233, 4263
Bse118I	RCCGGY	2 cut(s)	1748, 4793
Bse21I	CCTNAGG	2 cut(s)	643, 2960
Bse3DI	GCAATG	2 cut(s)	2727, 3779
Bse8I	GATNNNNATC	2 cut(s)	906, 4203
BseAI	TCCGGA	1 cut(s)	3652
BseGI	GGATG	4 cut(s)	694, 1612, 3110, 4792
BseJI	GATNNNNATC	2 cut(s)	906, 4203
BseMI	GCAATG	2 cut(s)	2727, 3779
BseMII	CTCAG	7 cut(s)	119, 351, 1792, 2033, 2974, 3915, 4044
BseRI	GAGGAG	8 cut(s)	1308, 1512, 1673, 1867, 3571, 3780, 3819, 4257
BseSI	GKGCMC	1 cut(s)	3355
BseX3I	CGGCCG	1 cut(s)	4899
BseYI	CCCAGC	4 cut(s)	1536, 1695, 2865, 2897
BsgI	GTGCAG	3 cut(s)	876, 2184, 3094
Bsh1236I	CGCG	1 cut(s)	906
Bsh1285I	CGRYCG	1 cut(s)	4902
BshNI	GGYRCC	2 cut(s)	3869, 4807
BshTI	ACCGGT	1 cut(s)	1748
BsiEI	CGRYCG	1 cut(s)	4902
BsiHKCI	CYCGRG	1 cut(s)	4707
BsiSI	CCGG	5 cut(s)	132, 1749, 3653, 4301, 4794
BslFI	GGGAC	2 cut(s)	3395, 3997
BsmBI	CGTCTC	2 cut(s)	3294, 3451
BsmFI	GGGAC	2 cut(s)	3395, 3997
Bso31I	GGTCTC	1 cut(s)	4251
BsoBI	CYCGRG	1 cut(s)	4707
Bsp119I	TTCGAA	1 cut(s)	1754
Bsp120I	GGGCCC	1 cut(s)	3351
Bsp1286I	GDGCHC	3 cut(s)	528, 2468, 3355
Bsp13I	TCCGGA	1 cut(s)	3652
Bsp19I	CCATGG	2 cut(s)	2158, 3427
Bsp68I	TCGCGA	1 cut(s)	906
BspACI	CCGC	7 cut(s)	587, 1447, 1721, 2085, 4117, 4723, 4820
BspCNI	CTCAG	7 cut(s)	118, 352, 1791, 2034, 2973, 3916, 4043
BspEI	TCCGGA	1 cut(s)	3652
BspFNI	CGCG	1 cut(s)	906
BspHI	TCATGA	1 cut(s)	2809
BspMAI	CTGCAG	4 cut(s)	880, 1292, 1825, 3684
BspOI	GCTAGC	1 cut(s)	3713
BspT104I	TTCGAA	1 cut(s)	1754
BspT107I	GGYRCC	2 cut(s)	3869, 4807
BspTI	CTTAAG	2 cut(s)	1336, 1991
BspTNI	GGTCTC	1 cut(s)	4251
BsrDI	GCAATG	2 cut(s)	2727, 3779
BsrFI	RCCGGY	2 cut(s)	1748, 4793
BssAI	RCCGGY	2 cut(s)	1748, 4793
BssNAI	GTATAC	1 cut(s)	2264
BssNI	GRCGYC	2 cut(s)	3384, 3444
BssSI	CACGAG	3 cut(s)	293, 1269, 2421
BssT1I	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
Bst1107I	GTATAC	1 cut(s)	2264
Bst2BI	CACGAG	3 cut(s)	293, 1269, 2421
Bst4CI	ACNGT	9 cut(s)	355, 802, 1373, 2103, 2191, 3463, 3648, 3943, 4642
Bst6I	CTCTTC	5 cut(s)	609, 1538, 1631, 2511, 4537
BstACI	GRCGYC	2 cut(s)	3384, 3444
BstAFI	CTTAAG	2 cut(s)	1336, 1991
BstBAI	YACGTR	4 cut(s)	248, 820, 3219, 3569
BstBI	TTCGAA	1 cut(s)	1754
BstDEI	CTNAG	9 cut(s)	105, 360, 643, 1037, 1778, 2042, 2960, 3924, 4030
BstDSI	CCRYGG	2 cut(s)	2158, 3427
BstEII	GGTNACC	3 cut(s)	1484, 2768, 4516
BstENI	CCTNNNNNAGG	2 cut(s)	336, 915
BstF5I	GGATG	4 cut(s)	694, 1612, 3110, 4792
BstFNI	CGCG	1 cut(s)	906
BstHHI	GCGC	1 cut(s)	2737
BstMCI	CGRYCG	1 cut(s)	4902
BstNSI	RCATGY	5 cut(s)	119, 1163, 2011, 2564, 4085
BstPI	GGTNACC	3 cut(s)	1484, 2768, 4516
BstSFI	CTRYAG	5 cut(s)	876, 1288, 1821, 3006, 3680
BstSLI	GKGCMC	1 cut(s)	3355
BstUI	CGCG	1 cut(s)	906
BstV2I	GAAGAC	4 cut(s)	81, 2030, 2336, 3126
BstX2I	RGATCY	3 cut(s)	777, 1547, 2059
BstXI	CCANNNNNNTGG	3 cut(s)	849, 1536, 3608
BstYI	RGATCY	3 cut(s)	777, 1547, 2059
BstZ17I	GTATAC	1 cut(s)	2264
BstZI	CGGCCG	1 cut(s)	4899
Bsu36I	CCTNAGG	2 cut(s)	643, 2960
BsuI	GTATCC	3 cut(s)	1702, 4275, 4546
BtgI	CCRYGG	2 cut(s)	2158, 3427
BtrI	CACGTC	2 cut(s)	1346, 3254
BtsCI	GGATG	4 cut(s)	694, 1612, 3110, 4792
BtsI	GCAGTG	2 cut(s)	1445, 3095
BtsIMutI	CAGTG	7 cut(s)	1445, 2664, 2745, 3095, 3850, 4379, 4541
BtuMI	TCGCGA	1 cut(s)	906
CaiI	CAGNNNCTG	1 cut(s)	2333
CciI	TCATGA	1 cut(s)	2809
CfoI	GCGC	1 cut(s)	2737
Cfr10I	RCCGGY	2 cut(s)	1748, 4793
Cfr13I	GGNCC	9 cut(s)	55, 1596, 2537, 2788, 2900, 3351, 3352, 3954, 4373
CseI	GACGC	5 cut(s)	485, 1618, 2217, 2661, 3392
CsiI	ACCWGGT	2 cut(s)	3600, 4150
CspAI	ACCGGT	1 cut(s)	1748
CspCI	CAANNNNNGTGG	2 cut(s)	3829, 3864
DdeI	CTNAG	9 cut(s)	105, 360, 643, 1037, 1778, 2042, 2960, 3924, 4030
DraI	TTTAAA	2 cut(s)	1095, 4738
EaeI	YGGCCR	2 cut(s)	4833, 4899
EagI	CGGCCG	1 cut(s)	4899
Eam1104I	CTCTTC	5 cut(s)	609, 1538, 1631, 2511, 4537
EarI	CTCTTC	5 cut(s)	609, 1538, 1631, 2511, 4537
EclXI	CGGCCG	1 cut(s)	4899
Eco130I	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
Eco147I	AGGCCT	1 cut(s)	3593
Eco24I	GRGCYC	3 cut(s)	528, 2468, 3355
Eco31I	GGTCTC	1 cut(s)	4251
Eco32I	GATATC	1 cut(s)	487
Eco47I	GGWCC	4 cut(s)	55, 1596, 2788, 4373
Eco52I	CGGCCG	1 cut(s)	4899
Eco57I	CTGAAG	6 cut(s)	371, 593, 1269, 2558, 2835, 4127
Eco72I	CACGTG	3 cut(s)	248, 3219, 3569
Eco81I	CCTNAGG	2 cut(s)	643, 2960
Eco88I	CYCGRG	1 cut(s)	4707
Eco91I	GGTNACC	3 cut(s)	1484, 2768, 4516
EcoNI	CCTNNNNNAGG	2 cut(s)	336, 915
EcoO109I	RGGNCCY	3 cut(s)	2537, 3351, 3352
EcoO65I	GGTNACC	3 cut(s)	1484, 2768, 4516
EcoRI	GAATTC	2 cut(s)	727, 1134
EcoRV	GATATC	1 cut(s)	487
EcoT14I	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
EcoT22I	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992
EcoT38I	GRGCYC	3 cut(s)	528, 2468, 3355
ErhI	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
Esp3I	CGTCTC	2 cut(s)	3294, 3451
FalI	AAGNNNNNCTT	6 cut(s)	73, 105, 452, 484, 2391, 2423
FaqI	GGGAC	2 cut(s)	3395, 3997
FauI	CCCGC	3 cut(s)	1714, 4730, 4813
FauNDI	CATATG	1 cut(s)	2907
FbaI	TGATCA	2 cut(s)	1582, 2824
FblI	GTMKAC	3 cut(s)	1875, 2263, 4572
FokI	GGATG	4 cut(s)	681, 1599, 3097, 4799
FriOI	GRGCYC	3 cut(s)	528, 2468, 3355
GlaI	GCGC	1 cut(s)	2736
GsaI	CCCAGC	4 cut(s)	1540, 1699, 2869, 2901
GsuI	CTGGAG	4 cut(s)	1406, 2724, 4385, 4575
HapII	CCGG	5 cut(s)	132, 1749, 3653, 4301, 4794
HgaI	GACGC	5 cut(s)	485, 1618, 2217, 2661, 3392
HhaI	GCGC	1 cut(s)	2737
Hin1I	GRCGYC	2 cut(s)	3384, 3444
Hin6I	GCGC	1 cut(s)	2735
HinP1I	GCGC	1 cut(s)	2735
HincII	GTYRAC	5 cut(s)	309, 1110, 1674, 2692, 3442
HindII	GTYRAC	5 cut(s)	309, 1110, 1674, 2692, 3442
HpaII	CCGG	5 cut(s)	132, 1749, 3653, 4301, 4794
Hpy99I	CGWCG	2 cut(s)	684, 831
HpyCH4III	ACNGT	9 cut(s)	355, 802, 1373, 2103, 2191, 3463, 3648, 3943, 4642
HpyF3I	CTNAG	9 cut(s)	105, 360, 643, 1037, 1778, 2042, 2960, 3924, 4030
Hsp92I	GRCGYC	2 cut(s)	3384, 3444
HspAI	GCGC	1 cut(s)	2735
Kpn2I	TCCGGA	1 cut(s)	3652
KpnI	GGTACC	1 cut(s)	4811
Ksp22I	TGATCA	2 cut(s)	1582, 2824
MabI	ACCWGGT	2 cut(s)	3600, 4150
MfeI	CAATTG	2 cut(s)	1017, 1139
MflI	RGATCY	3 cut(s)	777, 1547, 2059
MhlI	GDGCHC	3 cut(s)	528, 2468, 3355
MlsI	TGGCCA	1 cut(s)	4835
MluNI	TGGCCA	1 cut(s)	4835
MlyI	GAGTC	1 cut(s)	1000
MmeI	TCCRAC	3 cut(s)	33, 4273, 4401
Mox20I	TGGCCA	1 cut(s)	4835
Mph1103I	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992
MroI	TCCGGA	1 cut(s)	3652
MroXI	GAANNNNTTC	2 cut(s)	2586, 2848
MscI	TGGCCA	1 cut(s)	4835
MslI	CAYNNNNRTG	2 cut(s)	818, 820
Msp20I	TGGCCA	1 cut(s)	4835
MspA1I	CMGCKG	1 cut(s)	11
MspCI	CTTAAG	2 cut(s)	1336, 1991
MspI	CCGG	5 cut(s)	132, 1749, 3653, 4301, 4794
MunI	CAATTG	2 cut(s)	1017, 1139
MvnI	CGCG	1 cut(s)	906
NciI	CCSGG	1 cut(s)	4301
NcoI	CCATGG	2 cut(s)	2158, 3427
NdeI	CATATG	1 cut(s)	2907
NheI	GCTAGC	1 cut(s)	3709
NmeAIII	GCCGAG	4 cut(s)	3739, 4204, 4435, 4877
NmuCI	GTSAC	6 cut(s)	46, 355, 473, 598, 2768, 3197
NruI	TCGCGA	1 cut(s)	906
NsiI	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992
NspI	RCATGY	5 cut(s)	119, 1163, 2011, 2564, 4085
NspV	TTCGAA	1 cut(s)	1754
OliI	CACNNNNGTG	1 cut(s)	818
PaeI	GCATGC	1 cut(s)	1163
PaeR7I	CTCGAG	1 cut(s)	4707
PagI	TCATGA	1 cut(s)	2809
PceI	AGGCCT	1 cut(s)	3593
PciI	ACATGT	2 cut(s)	115, 2007
PcsI	WCGNNNNNNNCGW	1 cut(s)	4144
PdmI	GAANNNNTTC	2 cut(s)	2586, 2848
PflFI	GACNNNGTC	1 cut(s)	3533
PflMI	CCANNNNNTGG	2 cut(s)	1605, 2624
PfoI	TCCNGGA	1 cut(s)	4623
PinAI	ACCGGT	1 cut(s)	1748
PleI	GAGTC	1 cut(s)	999
PmaCI	CACGTG	3 cut(s)	248, 3219, 3569
PmlI	CACGTG	3 cut(s)	248, 3219, 3569
PpsI	GAGTC	1 cut(s)	999
Ppu21I	YACGTR	4 cut(s)	248, 820, 3219, 3569
PscI	ACATGT	2 cut(s)	115, 2007
PshBI	ATTAAT	1 cut(s)	453
PsiI	TTATAA	1 cut(s)	915
PspCI	CACGTG	3 cut(s)	248, 3219, 3569
PspEI	GGTNACC	3 cut(s)	1484, 2768, 4516
PspFI	CCCAGC	4 cut(s)	1536, 1695, 2865, 2897
PspOMI	GGGCCC	1 cut(s)	3351
PspPI	GGNCC	9 cut(s)	55, 1596, 2537, 2788, 2900, 3351, 3352, 3954, 4373
PstI	CTGCAG	4 cut(s)	880, 1292, 1825, 3684
PstNI	CAGNNNCTG	1 cut(s)	2333
PsuI	RGATCY	3 cut(s)	777, 1547, 2059
PsyI	GACNNNGTC	1 cut(s)	3533
PvuII	CAGCTG	1 cut(s)	11
RruI	TCGCGA	1 cut(s)	906
RseI	CAYNNNNRTG	2 cut(s)	818, 820
Sau96I	GGNCC	9 cut(s)	55, 1596, 2537, 2788, 2900, 3351, 3352, 3954, 4373
ScaI	AGTACT	2 cut(s)	932, 1501
SchI	GAGTC	1 cut(s)	1000
SduI	GDGCHC	3 cut(s)	528, 2468, 3355
SexAI	ACCWGGT	2 cut(s)	3600, 4150
SfcI	CTRYAG	5 cut(s)	876, 1288, 1821, 3006, 3680
Sfr274I	CTCGAG	1 cut(s)	4707
SfuI	TTCGAA	1 cut(s)	1754
SinI	GGWCC	4 cut(s)	55, 1596, 2788, 4373
SlaI	CTCGAG	1 cut(s)	4707
SmiMI	CAYNNNNRTG	2 cut(s)	818, 820
SmlI	CTYRAG	6 cut(s)	1336, 1898, 1991, 2352, 3146, 4707
SmoI	CTYRAG	6 cut(s)	1336, 1898, 1991, 2352, 3146, 4707
SpeI	ACTAGT	1 cut(s)	4567
SphI	GCATGC	1 cut(s)	1163
SseBI	AGGCCT	1 cut(s)	3593
SsiI	CCGC	7 cut(s)	587, 1447, 1721, 2085, 4117, 4723, 4820
SspI	AATATT	2 cut(s)	255, 4037
StuI	AGGCCT	1 cut(s)	3593
StyI	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
TaaI	ACNGT	9 cut(s)	355, 802, 1373, 2103, 2191, 3463, 3648, 3943, 4642
TatI	WGTACW	5 cut(s)	930, 1499, 2871, 3563, 4692
TauI	GCSGC	4 cut(s)	589, 1450, 2087, 4119
TscAI	CASTG	7 cut(s)	1445, 2664, 2745, 3102, 3850, 4386, 4548
TseFI	GTSAC	6 cut(s)	46, 355, 473, 598, 2768, 3197
Tsp45I	GTSAC	6 cut(s)	46, 355, 473, 598, 2768, 3197
TspGWI	ACGGA	2 cut(s)	225, 1605
TspRI	CASTG	7 cut(s)	1445, 2664, 2745, 3102, 3850, 4386, 4548
Tth111I	GACNNNGTC	1 cut(s)	3533
Van91I	CCANNNNNTGG	2 cut(s)	1605, 2624
Vha464I	CTTAAG	2 cut(s)	1336, 1991
VpaK11BI	GGWCC	4 cut(s)	55, 1596, 2788, 4373
VspI	ATTAAT	1 cut(s)	453
XagI	CCTNNNNNAGG	2 cut(s)	336, 915
XapI	RAATTY	7 cut(s)	536, 727, 967, 1134, 1805, 4051, 4094
XceI	RCATGY	5 cut(s)	119, 1163, 2011, 2564, 4085
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4282
XhoI	CTCGAG	1 cut(s)	4707
XmiI	GTMKAC	3 cut(s)	1875, 2263, 4572
XmnI	GAANNNNTTC	2 cut(s)	2586, 2848
ZraI	GACGTC	1 cut(s)	3445
ZrmI	AGTACT	2 cut(s)	932, 1501
Zsp2I	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.