Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0008066

Physical Location & Seq

Genomic Coordinates Reverse (-)

177511 .. 184563

Gene Structure 7053 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g00290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4095 bp

ATGGAATCAGCTGGTGGTGGTATAAGGGAGACATCTGTTGTAGTTGTGACGTTGGATCCTGGTGAAGTATATATAATTGCGAGTTTGTCTTCCAGGCTCGACACTCAGGTTATACAAATTGATCCCACAACCGGAGTGCTTCGCTATAATGCAAAGCCAGGATTTGATGTTTTCAAATCCGAAAAGGAAGCATTGGAATATGTTACGAACGGATCACATTGGTTGTGTAAGAATACAACCTATGCGCGTGCAATATTGGGTTATGCTGCTTTGGGCAGCATTGGTTTGCTTCTCGTGGCTACCAAGTTGACTGCTAGTGTTCCGAATTTGCCTGGTGGAGGGTGCATTTATACGGTGACTGAGAGCCAATGGATCAAGATTCAACTTCAGAATCCACAACCACAAGGGAAAGGAGAAGAAAAGAATGTTAATGAATTAACCGATCTTGACATTAATGGGAAGCACTACTTTTGTGACGCAAGGGATATCACTCAACCATTTCCTAGCCGTATGTGCTTGCGTGAGCCTGATGATGAATTTGTTTGGAACGCCTGGTTCTCGATGCCTTTCAAAAACATTGGGCTGCCACAGCATTGTGTCACTCTTCTTCAGGGTTTTGCAGAGTGTCGAAGTTTTGGAACCTTAGGGAAGGTGGAAGGAATTGTTGCTCTCATAGCTCGCCGTAGCAGGCTGCATCCTGGTACTCGGTACTTGGCTAGGGGATTGAATTCATGTTTTAGCACAGGAAATGAAGTGGAGTGTGAGCAACTTGTATGGATCCCTAGAAGGGCTGGTCAAACAGTTCCTTTTAACACATACGTGTGGCGACGGGGTACAATTCCAATTTGGTGGGGTGCAGAGTTAAAGATTACTGCTGCAGAAGCAGAAATATACGTTTCGGATCGCGATCCTTATAAAGGAAGTTCGGAGTACTACCAGAGGTTGAGTAAGAGGTATGATGCACGAAATTTTGACGTGGCTGTTGGGGGGAGTCAGAATAGAAAGGCGTTTGTTCCAATTGTATGCATCAACTTGCTTAGGAGTGGAGAAGGAAAGTCAGAATGTATTTTAGTTCAACATTTTAAGAAATCTTTAAACTACATCAAATCAACCAGAAAACTTCCTTATACTCGAATTGAATTGATAAATTATGACTGGCATGCCAGTATAAAGTTAAGAGGTGAACAGCAAACCATCGAAGGATTATGGAAACATCTAAAAGCACCCACTACTTCCATAGGCATTTCTGAAGGAGATTTTTTGCCTTCACGAGAAAGAATTAAGGACTGCAGGGGAGAAATTATCTACAATGATGACTTTGAAGGTGCTTTCTGCTTAAGATCACGTCAAAATGGTGTGATACGTTTCAACTGTGCTGATTCTTTGGATAGGACAAATGCTGCAAGTTATTTTGGCTCCCTCCAGGTTTTTGTGGAGCAGTGTAGGCGGCTTTGTATATCACTTGATAGTGATTTGGCATTTGGTTATCAGTCAATGAGTACTATTGGTGGCTATACAGCTCCTCTGCCACCAGGCTGGGAGAAGAGATCCGATGCAGTAACAGGGAAAACCTTTTATATTGATCACAATACTAGGACCACAACATGGACGCATCCGTGTCCCGATGAACCTTGGAAGAGATTTGATATGGCATTTGAGGAGTTCAAGAGGTCAACAATTTTATCACCAGTATCCCAGCTTGCTGATATTTTTCTGCTTGCGGGTGATATTCATGCAACACTTTATACCGGTTCGAAAGCTATGCATAGCCAAATCCTCAGCATCTTCAATGACGATGCAGGAAAATTCAAACAATTTTCTGCAGCTCAGAATATGAAAATTACTTTGCAGAGGAGATATAAAAATGCAGTTGTTGACAGCTCTCGTCAAAAGCAACTTGAGATATTTCTTGGAATGAGACTATTCAAGCATCTTCCATCAGTTTCTTTTCACCCTCTTAATGTAGTCTCTCGACCATCTGGTTTCTTCCTTAAGCCAGTTGCTAACATGTTTCCAAGTTCCAAAGGTGGAGCCAGTCTTCTGAGTTTCAAGAGAAAGGATCTAGTCTGGGTTTGCCCACAGGCCGCAGATGTTGTTGAACTTTTTATCTATCTAGGTGAACCTTGCCATGTTTGTCAGCTTCTTCTCACAATATCCCATGGTGCAGATGATTCAACATATCCATCAACAGTTGATGTAAGGACAGGACGCTCTTTAGATGGGCTAAAACTTGTCTTGGAGGGTGCTTCAATACCTCAGTGTATACACGGAACAAACCTCTTGATACCCTTAACAGGAGCAGTTAGTCCAGAGGATATGGCTGTAACAGGAGCTGGTTCACGTCTTCATGCTCAAGATACATCTACCCTTCCATTATTGTATGATTTTGAAGAACTGGAAGGAGAACTTGACTTCCTTACTCGTGTAGTTGCCCTTACATTTTATCCTGCTGTCTCTGGAAAGAGCCCTATCACTCTTGGTGAGATAGAAGTCCTTGGAGTTTCTCTTCCTTGGAGAGGTGTATTTACTAACGAAGACCCTGGTGCAAGACTACCTGAACATGCTAAAAACCTTCAGAATGAAAACCTTCATTTTTCCTCTGGGTCAAAAACCAATCCATTTTCTGGTGCTTCATTTACTGAAAATGTGCTGCTACCAGTGCAACCAAGTTCCTCTGCCAACAATTTAGTTGACCTATTGACTGGAGAGGTGGTGCTTTCAGAACACATTGCTGCGCCAGTGATAGTAAATGCTGAGGACAAGGGAGGTGACTTTCTTGATTTCTTGGACCAAGCTATTGTTGAATATCATGGTGCTGAAACTGATCATAAATCACATGATGGAAAACCTTCAGATAGCAGTTCCCAGCAGTACATTGATTGTTTGAAATCTGTTGCTGGGCCACATATGGAAAAGAAACTAAACTTCATGGAAGCCATGAAACTTGAAATTGAACGCCTCAGGCTGAACATTTCTGCTGCTGAAAGGGATAGAGCTTTGTTATCTCTAGGAACTGATCCTGCTACTATAAACCCTAATGTTTTACTTGATGAACGATACATGGGAAGATTGTGCAGAGTTGCAAACTCCCTTGCACTGCTTGGACAAGCATCCTTGGAAGACAAAATTACATCTGCTGTTGGTCTTGAGACCACTGATGATAATGAGATAGATTTTTGGAATATAACTAGATTTGGTGACCACTGTTATGGTGGCACATGTGAGGTTCGTGCTGAAACTAATGCACCTACACGTCCTTCCTTTTTGGAATCAGGCGGAGTTTCCCCGTCTCTCTTGTCATGTTCCCAATGTGAAAGGAAAGTTTGTAAAGTTTGCTGTGCTGGTAGAGGGGCCCTTCTGGTTGCAGGCTATGGGTCAAGGGAGGCCATGAGTTACAATGGTGTGGTAAATCAGGAGGGATCAGGCCATGGTTTCCAGGCTGATGTCTCTACAAATCGTACAGTAGTGCTGGATAGTGTTATTTGTAAAAGATGCTGCCATGATATTGTGCTTGATGCATTGATCTTGGACTACGTCAGGGTCTTGATAAGCATGAGGAGAAGTACACGTGCAGATTCAGCTGCCCATGAGGCCTTGAACCAGGTGATTGGATATTCAGTAAGGAATTGTCTTTATGAAGGGAAACAGTCTCCGGATAGAAAGAGAACTATTAAAGTTCTGCAGAAATTACTTGGCAGAGAGGAATCGCTAGCCGAGTTTCCATTTGCCAGCTTTTTACACTCGGTTGAAACGGGAGCAGATTCAGCACCATTCTTGTCATTGCTTGCTCCTCTTGAATCTGGAACACGCGATTCATACTGGAAATCTCCTCCTAGTACCACCAATGTTGAGTTCATTATTGTTCTTGGCACCCTTTCTGATGTTAGTGGGGTTGTTTTGCTTATTAGTCCATGTGGTTATTCTTTGGCTGATGCACCCACCGTGCAAATCTGGGCCAGCAATAAAATACACAAGGAAGAAAGGTCATGCATGGGAAAATGGGATTTGCAGTCCTCTGTTGATCGATCACGTCTTCCTCGGAATATTATGCACCAGAAAAGTCGGTTAGAGAAAATGAAGTACCGAGGCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0031348 GO:0033612 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0060548 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Syja_N WW

Protein Analysis

1365

Amino Acids

151.02

Weight (kDa)

6.49

Isoelectric Point (pI)

43.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syja_N	PF02383	84 - 390	8.5e-40	SacI homology domain
WW	PF00397	509 - 539	6.1e-12	WW domain
SAC9_GBDL_1st	PF24790	653 - 838	1.8e-98	SAC9 first GBDL domain
SAC9_C8D	PF24791	1063 - 1173	5.9e-18	SAC9 C8D domain
SAC9_GBDL_2nd	PF24789	1234 - 1343	1.1e-54	SAC9 second GBDL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59770 AT3G59770 AT3G59770 AT3G59770
fragaria_vesca	FvH4_1g00530 FvH4_1g00530
malus_domestica	MD02G1004400.v1.1 MD15G1148100.v1.1
prunus_persica	Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1
pyrus_communis	pycom02g00290 pycom15g13240
rosa_chinensis	RchiOBHm_Chr2g0085161
rosa_laevigata	RLG00000015661
rosa_multiflora	Rmu_sc0008647.1_g000009
rosa_roxburghii	Rroxscaffold_2G00155600
rosa_rugosa	Rorug01G0458000
rosa_samantha	Rh2AG006300 Rh2BG007100 Rh2CG007900 Rh2DG008100
rosa_wichuraiana	Rw2G000600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	915
AccB1I	GGYRCC	1 cut(s)	3869
AccB7I	CCANNNNNTGG	2 cut(s)	1605, 2624
AccI	GTMKAC	1 cut(s)	2263
AccII	CGCG	3 cut(s)	247, 906, 3810
AccIII	TCCGGA	1 cut(s)	3652
AciI	CCGC	4 cut(s)	1447, 1721, 2085, 3276
AcsI	RAATTY	5 cut(s)	325, 536, 727, 967, 1805
AcuI	CTGAAG	5 cut(s)	371, 593, 1269, 2558, 2835
AcvI	CACGTG	1 cut(s)	3569
AfiI	CCNNNNNNNGG	8 cut(s)	131, 338, 787, 917, 1605, 2294, 2516, 2624
AflII	CTTAAG	2 cut(s)	1336, 1991
AflIII	ACRYGT	5 cut(s)	819, 2007, 3218, 3251, 3566
AgeI	ACCGGT	1 cut(s)	1748
AjiI	CACGTC	5 cut(s)	976, 1346, 2342, 3254, 4031
AjuI	GAANNNNNNNTTGG	2 cut(s)	3836, 3868
AleI	CACNNNNGTG	1 cut(s)	818
AloI	GAACNNNNNNTCC	2 cut(s)	2397, 2429
Alw26I	GTCTC	8 cut(s)	23, 1912, 1972, 2458, 3143, 3294, 3451, 3654
AlwNI	CAGNNNCTG	1 cut(s)	2333
Aor13HI	TCCGGA	1 cut(s)	3652
AoxI	GGCC	7 cut(s)	2082, 2900, 3351, 3384, 3424, 3591, 3954
ApaI	GGGCCC	1 cut(s)	3355
ApoI	RAATTY	5 cut(s)	325, 536, 727, 967, 1805
AseI	ATTAAT	1 cut(s)	453
AsiGI	ACCGGT	1 cut(s)	1748
Asp700I	GAANNNNTTC	2 cut(s)	2586, 2848
AspLEI	GCGC	2 cut(s)	247, 2737
AspS9I	GGNCC	6 cut(s)	1596, 2788, 2900, 3351, 3352, 3954
AsuII	TTCGAA	1 cut(s)	1754
AsuNHI	GCTAGC	1 cut(s)	3709
AvaII	GGWCC	2 cut(s)	1596, 2788
AxyI	CCTNAGG	2 cut(s)	643, 2960
BaeGI	GKGCMC	1 cut(s)	3355
BamHI	GGATCC	2 cut(s)	55, 777
BanI	GGYRCC	1 cut(s)	3869
BanII	GRGCYC	2 cut(s)	2468, 3355
BauI	CACGAG	3 cut(s)	293, 1269, 2421
BbrPI	CACGTG	1 cut(s)	3569
BbsI	GAAGAC	6 cut(s)	81, 2030, 2336, 2541, 3126, 4025
BbvCI	CCTCAGC	2 cut(s)	1778, 2754
BccI	CCATC	6 cut(s)	1202, 1945, 1984, 2191, 2213, 2834
BceAI	ACGGC	2 cut(s)	492, 666
BcgI	CGANNNNNNTGC	2 cut(s)	1744, 1778
BciVI	GTATCC	1 cut(s)	1702
BclI	TGATCA	2 cut(s)	1582, 2824
BcoDI	GTCTC	8 cut(s)	23, 1912, 1972, 2458, 3143, 3294, 3451, 3654
BfmI	CTRYAG	4 cut(s)	876, 1288, 1821, 3680
BfrI	CTTAAG	2 cut(s)	1336, 1991
BfuI	GTATCC	1 cut(s)	1702
BglI	GCCNNNNNGGC	1 cut(s)	3590
BmcAI	AGTACT	2 cut(s)	932, 1501
Bme18I	GGWCC	2 cut(s)	1596, 2788
BmgBI	CACGTC	5 cut(s)	976, 1346, 2342, 3254, 4031
BmgT120I	GGNCC	6 cut(s)	1596, 2788, 2900, 3351, 3352, 3954
BmiI	GGNNCC	8 cut(s)	57, 640, 779, 1417, 2032, 3352, 3353, 3871
BmtI	GCTAGC	1 cut(s)	3713
BpiI	GAAGAC	6 cut(s)	81, 2030, 2336, 2541, 3126, 4025
BpmI	CTGGAG	2 cut(s)	1406, 2724
Bpu10I	CCTNAGC	3 cut(s)	1037, 1778, 2754
Bpu14I	TTCGAA	1 cut(s)	1754
BpuEI	CTTGAG	3 cut(s)	1919, 2337, 3167
Bsa29I	ATCGAT	1 cut(s)	4024
BsaAI	YACGTR	2 cut(s)	820, 3569
BsaBI	GATNNNNATC	1 cut(s)	906
BsaI	GGTCTC	1 cut(s)	3143
BsaJI	CCNNGG	9 cut(s)	1631, 2158, 2494, 2510, 2539, 3114, 3427, 4037, 4084
BsaWI	WCCGGW	3 cut(s)	131, 1748, 3652
BsaXI	ACNNNNNCTCC	2 cut(s)	2697, 2727
Bsc4I	CCNNNNNNNGG	8 cut(s)	131, 338, 787, 917, 1605, 2294, 2516, 2624
Bse118I	RCCGGY	1 cut(s)	1748
Bse21I	CCTNAGG	2 cut(s)	643, 2960
Bse3DI	GCAATG	2 cut(s)	2727, 3779
Bse8I	GATNNNNATC	1 cut(s)	906
BseAI	TCCGGA	1 cut(s)	3652
BseCI	ATCGAT	1 cut(s)	4024
BseDI	CCNNGG	9 cut(s)	1631, 2158, 2494, 2510, 2539, 3114, 3427, 4037, 4084
BseGI	GGATG	3 cut(s)	694, 1612, 3110
BseJI	GATNNNNATC	1 cut(s)	906
BseLI	CCNNNNNNNGG	8 cut(s)	131, 338, 787, 917, 1605, 2294, 2516, 2624
BseMI	GCAATG	2 cut(s)	2727, 3779
BseMII	CTCAG	8 cut(s)	119, 351, 1792, 1841, 2033, 2270, 2745, 2974
BseRI	GAGGAG	6 cut(s)	1512, 1673, 1867, 3571, 3780, 3819
BseSI	GKGCMC	1 cut(s)	3355
BseYI	CCCAGC	4 cut(s)	1536, 1695, 2865, 2897
BsgI	GTGCAG	4 cut(s)	876, 2184, 3094, 3591
Bsh1236I	CGCG	3 cut(s)	247, 906, 3810
BshFI	GGCC	7 cut(s)	2084, 2902, 3353, 3386, 3426, 3593, 3956
BshNI	GGYRCC	1 cut(s)	3869
BshTI	ACCGGT	1 cut(s)	1748
BshVI	ATCGAT	1 cut(s)	4024
BsiSI	CCGG	3 cut(s)	132, 1749, 3653
BslFI	GGGAC	1 cut(s)	1605
BslI	CCNNNNNNNGG	8 cut(s)	131, 338, 787, 917, 1605, 2294, 2516, 2624
BsmAI	GTCTC	8 cut(s)	23, 1912, 1972, 2458, 3143, 3294, 3451, 3654
BsmBI	CGTCTC	1 cut(s)	3294
BsmFI	GGGAC	1 cut(s)	1605
BsnI	GGCC	7 cut(s)	2084, 2902, 3353, 3386, 3426, 3593, 3956
Bso31I	GGTCTC	1 cut(s)	3143
Bsp119I	TTCGAA	1 cut(s)	1754
Bsp120I	GGGCCC	1 cut(s)	3351
Bsp1286I	GDGCHC	2 cut(s)	2468, 3355
Bsp13I	TCCGGA	1 cut(s)	3652
Bsp19I	CCATGG	2 cut(s)	2158, 3427
Bsp68I	TCGCGA	1 cut(s)	906
BspACI	CCGC	4 cut(s)	1447, 1721, 2085, 3276
BspANI	GGCC	7 cut(s)	2084, 2902, 3353, 3386, 3426, 3593, 3956
BspCNI	CTCAG	8 cut(s)	118, 352, 1791, 1840, 2034, 2269, 2746, 2973
BspDI	ATCGAT	1 cut(s)	4024
BspEI	TCCGGA	1 cut(s)	3652
BspFNI	CGCG	3 cut(s)	247, 906, 3810
BspLI	GGNNCC	8 cut(s)	57, 640, 779, 1417, 2032, 3352, 3353, 3871
BspMAI	CTGCAG	4 cut(s)	880, 1292, 1825, 3684
BspOI	GCTAGC	1 cut(s)	3713
BspT104I	TTCGAA	1 cut(s)	1754
BspT107I	GGYRCC	1 cut(s)	3869
BspTI	CTTAAG	2 cut(s)	1336, 1991
BspTNI	GGTCTC	1 cut(s)	3143
BsrDI	GCAATG	2 cut(s)	2727, 3779
BsrFI	RCCGGY	1 cut(s)	1748
BssAI	RCCGGY	1 cut(s)	1748
BssECI	CCNNGG	9 cut(s)	1631, 2158, 2494, 2510, 2539, 3114, 3427, 4037, 4084
BssNAI	GTATAC	1 cut(s)	2264
BssSI	CACGAG	3 cut(s)	293, 1269, 2421
BssT1I	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
Bst1107I	GTATAC	1 cut(s)	2264
Bst2BI	CACGAG	3 cut(s)	293, 1269, 2421
Bst4CI	ACNGT	8 cut(s)	355, 802, 1373, 2191, 3206, 3463, 3648, 3943
Bst6I	CTCTTC	4 cut(s)	609, 1538, 1631, 2511
BstAFI	CTTAAG	2 cut(s)	1336, 1991
BstBAI	YACGTR	2 cut(s)	820, 3569
BstBI	TTCGAA	1 cut(s)	1754
BstDSI	CCRYGG	2 cut(s)	2158, 3427
BstEII	GGTNACC	1 cut(s)	3197
BstENI	CCTNNNNNAGG	3 cut(s)	336, 915, 2514
BstF5I	GGATG	3 cut(s)	694, 1612, 3110
BstFNI	CGCG	3 cut(s)	247, 906, 3810
BstHHI	GCGC	2 cut(s)	247, 2737
BstMAI	GTCTC	8 cut(s)	23, 1912, 1972, 2458, 3143, 3294, 3451, 3654
BstMWI	GCNNNNNNNGC	9 cut(s)	513, 589, 674, 881, 1444, 2659, 3578, 3590, 3764
BstNSI	RCATGY	5 cut(s)	1163, 2011, 2564, 3222, 4093
BstPI	GGTNACC	1 cut(s)	3197
BstSFI	CTRYAG	4 cut(s)	876, 1288, 1821, 3680
BstSLI	GKGCMC	1 cut(s)	3355
BstUI	CGCG	3 cut(s)	247, 906, 3810
BstV2I	GAAGAC	6 cut(s)	81, 2030, 2336, 2541, 3126, 4025
BstX2I	RGATCY	4 cut(s)	55, 777, 1547, 2059
BstXI	CCANNNNNNTGG	4 cut(s)	849, 1536, 3209, 3608
BstYI	RGATCY	4 cut(s)	55, 777, 1547, 2059
BstZ17I	GTATAC	1 cut(s)	2264
Bsu15I	ATCGAT	1 cut(s)	4024
Bsu36I	CCTNAGG	2 cut(s)	643, 2960
BsuI	GTATCC	1 cut(s)	1702
BsuRI	GGCC	7 cut(s)	2084, 2902, 3353, 3386, 3426, 3593, 3956
BsuTUI	ATCGAT	1 cut(s)	4024
BtgI	CCRYGG	2 cut(s)	2158, 3427
BtrI	CACGTC	5 cut(s)	976, 1346, 2342, 3254, 4031
BtsCI	GGATG	3 cut(s)	694, 1612, 3110
BtsI	GCAGTG	2 cut(s)	1445, 3095
BtsIMutI	CAGTG	7 cut(s)	1445, 2264, 2664, 2745, 3095, 3153, 3202
BtuMI	TCGCGA	1 cut(s)	906
CaiI	CAGNNNCTG	1 cut(s)	2333
CfoI	GCGC	2 cut(s)	247, 2737
Cfr10I	RCCGGY	1 cut(s)	1748
Cfr13I	GGNCC	6 cut(s)	1596, 2788, 2900, 3351, 3352, 3954
ClaI	ATCGAT	1 cut(s)	4024
CseI	GACGC	3 cut(s)	485, 1618, 2217
CsiI	ACCWGGT	1 cut(s)	3600
CspAI	ACCGGT	1 cut(s)	1748
CspCI	CAANNNNNGTGG	2 cut(s)	3829, 3864
DraI	TTTAAA	1 cut(s)	1095
Eam1104I	CTCTTC	4 cut(s)	609, 1538, 1631, 2511
EarI	CTCTTC	4 cut(s)	609, 1538, 1631, 2511
EciI	GGCGGA	1 cut(s)	3291
Eco130I	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
Eco147I	AGGCCT	1 cut(s)	3593
Eco24I	GRGCYC	2 cut(s)	2468, 3355
Eco31I	GGTCTC	1 cut(s)	3143
Eco32I	GATATC	1 cut(s)	487
Eco47I	GGWCC	2 cut(s)	1596, 2788
Eco57I	CTGAAG	5 cut(s)	371, 593, 1269, 2558, 2835
Eco72I	CACGTG	1 cut(s)	3569
Eco81I	CCTNAGG	2 cut(s)	643, 2960
Eco91I	GGTNACC	1 cut(s)	3197
EcoNI	CCTNNNNNAGG	3 cut(s)	336, 915, 2514
EcoO109I	RGGNCCY	2 cut(s)	3351, 3352
EcoO65I	GGTNACC	1 cut(s)	3197
EcoRI	GAATTC	1 cut(s)	727
EcoRV	GATATC	1 cut(s)	487
EcoT14I	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
EcoT22I	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992
EcoT38I	GRGCYC	2 cut(s)	2468, 3355
ErhI	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
Esp3I	CGTCTC	1 cut(s)	3294
FalI	AAGNNNNNCTT	4 cut(s)	452, 484, 2391, 2423
FaqI	GGGAC	1 cut(s)	1605
FauI	CCCGC	1 cut(s)	1714
FauNDI	CATATG	1 cut(s)	2907
FbaI	TGATCA	2 cut(s)	1582, 2824
FblI	GTMKAC	1 cut(s)	2263
FokI	GGATG	3 cut(s)	681, 1599, 3097
FriOI	GRGCYC	2 cut(s)	2468, 3355
GlaI	GCGC	2 cut(s)	246, 2736
GsaI	CCCAGC	4 cut(s)	1540, 1699, 2869, 2901
GsuI	CTGGAG	2 cut(s)	1406, 2724
HaeIII	GGCC	7 cut(s)	2084, 2902, 3353, 3386, 3426, 3593, 3956
HapII	CCGG	3 cut(s)	132, 1749, 3653
HgaI	GACGC	3 cut(s)	485, 1618, 2217
HhaI	GCGC	2 cut(s)	247, 2737
Hin6I	GCGC	2 cut(s)	245, 2735
HinP1I	GCGC	2 cut(s)	245, 2735
HincII	GTYRAC	4 cut(s)	309, 1674, 1876, 2692
HindII	GTYRAC	4 cut(s)	309, 1674, 1876, 2692
HpaII	CCGG	3 cut(s)	132, 1749, 3653
Hpy166II	GTNNAC	9 cut(s)	309, 1184, 1674, 1876, 2120, 2264, 2339, 2692, 3566
Hpy8I	GTNNAC	9 cut(s)	309, 1184, 1674, 1876, 2120, 2264, 2339, 2692, 3566
Hpy99I	CGWCG	1 cut(s)	831
HpyCH4III	ACNGT	8 cut(s)	355, 802, 1373, 2191, 3206, 3463, 3648, 3943
HpyF10VI	GCNNNNNNNGC	9 cut(s)	513, 589, 674, 881, 1444, 2659, 3578, 3590, 3764
HspAI	GCGC	2 cut(s)	245, 2735
Kpn2I	TCCGGA	1 cut(s)	3652
Ksp22I	TGATCA	2 cut(s)	1582, 2824
LmnI	GCTCC	8 cut(s)	1421, 1435, 1525, 2030, 2297, 2330, 3755, 3793
MabI	ACCWGGT	1 cut(s)	3600
MfeI	CAATTG	1 cut(s)	1017
MflI	RGATCY	4 cut(s)	55, 777, 1547, 2059
MhlI	GDGCHC	2 cut(s)	2468, 3355
MlyI	GAGTC	1 cut(s)	1000
MmeI	TCCRAC	1 cut(s)	33
Mph1103I	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992
MroI	TCCGGA	1 cut(s)	3652
MroXI	GAANNNNTTC	2 cut(s)	2586, 2848
MslI	CAYNNNNRTG	3 cut(s)	818, 820, 3207
MspA1I	CMGCKG	2 cut(s)	11, 3581
MspCI	CTTAAG	2 cut(s)	1336, 1991
MspI	CCGG	3 cut(s)	132, 1749, 3653
MunI	CAATTG	1 cut(s)	1017
MvnI	CGCG	3 cut(s)	247, 906, 3810
MwoI	GCNNNNNNNGC	9 cut(s)	513, 589, 674, 881, 1444, 2659, 3578, 3590, 3764
NcoI	CCATGG	2 cut(s)	2158, 3427
NdeI	CATATG	1 cut(s)	2907
NheI	GCTAGC	1 cut(s)	3709
NlaIV	GGNNCC	8 cut(s)	57, 640, 779, 1417, 2032, 3352, 3353, 3871
NmeAIII	GCCGAG	1 cut(s)	3739
NmuCI	GTSAC	6 cut(s)	46, 355, 473, 598, 2768, 3197
NruI	TCGCGA	1 cut(s)	906
NsiI	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992
NspI	RCATGY	5 cut(s)	1163, 2011, 2564, 3222, 4093
NspV	TTCGAA	1 cut(s)	1754
OliI	CACNNNNGTG	1 cut(s)	818
PaeI	GCATGC	1 cut(s)	1163
PceI	AGGCCT	1 cut(s)	3593
PciI	ACATGT	2 cut(s)	2007, 3218
PdmI	GAANNNNTTC	2 cut(s)	2586, 2848
PflFI	GACNNNGTC	1 cut(s)	3533
PflMI	CCANNNNNTGG	2 cut(s)	1605, 2624
PinAI	ACCGGT	1 cut(s)	1748
PleI	GAGTC	1 cut(s)	999
PmaCI	CACGTG	1 cut(s)	3569
PmlI	CACGTG	1 cut(s)	3569
PpsI	GAGTC	1 cut(s)	999
Ppu21I	YACGTR	2 cut(s)	820, 3569
PscI	ACATGT	2 cut(s)	2007, 3218
PshBI	ATTAAT	1 cut(s)	453
PsiI	TTATAA	1 cut(s)	915
PspCI	CACGTG	1 cut(s)	3569
PspEI	GGTNACC	1 cut(s)	3197
PspFI	CCCAGC	4 cut(s)	1536, 1695, 2865, 2897
PspN4I	GGNNCC	8 cut(s)	57, 640, 779, 1417, 2032, 3352, 3353, 3871
PspOMI	GGGCCC	1 cut(s)	3351
PspPI	GGNCC	6 cut(s)	1596, 2788, 2900, 3351, 3352, 3954
PstI	CTGCAG	4 cut(s)	880, 1292, 1825, 3684
PstNI	CAGNNNCTG	1 cut(s)	2333
PsuI	RGATCY	4 cut(s)	55, 777, 1547, 2059
PsyI	GACNNNGTC	1 cut(s)	3533
PvuII	CAGCTG	2 cut(s)	11, 3581
RruI	TCGCGA	1 cut(s)	906
RseI	CAYNNNNRTG	3 cut(s)	818, 820, 3207
Sau96I	GGNCC	6 cut(s)	1596, 2788, 2900, 3351, 3352, 3954
ScaI	AGTACT	2 cut(s)	932, 1501
SchI	GAGTC	1 cut(s)	1000
SduI	GDGCHC	2 cut(s)	2468, 3355
SexAI	ACCWGGT	1 cut(s)	3600
SfcI	CTRYAG	4 cut(s)	876, 1288, 1821, 3680
SfuI	TTCGAA	1 cut(s)	1754
SinI	GGWCC	2 cut(s)	1596, 2788
SmiMI	CAYNNNNRTG	3 cut(s)	818, 820, 3207
SmlI	CTYRAG	5 cut(s)	1336, 1898, 1991, 2352, 3146
SmoI	CTYRAG	5 cut(s)	1336, 1898, 1991, 2352, 3146
SphI	GCATGC	1 cut(s)	1163
SseBI	AGGCCT	1 cut(s)	3593
SsiI	CCGC	4 cut(s)	1447, 1721, 2085, 3276
SspI	AATATT	2 cut(s)	255, 4045
StuI	AGGCCT	1 cut(s)	3593
StyI	CCWWGG	6 cut(s)	1631, 2158, 2494, 2510, 3114, 3427
TaaI	ACNGT	8 cut(s)	355, 802, 1373, 2191, 3206, 3463, 3648, 3943
TaqI	TCGA	8 cut(s)	99, 560, 628, 1132, 1197, 1754, 1972, 4024
TatI	WGTACW	4 cut(s)	930, 1499, 2871, 3563
TauI	GCSGC	2 cut(s)	1450, 2087
TscAI	CASTG	7 cut(s)	1445, 2264, 2664, 2745, 3102, 3160, 3209
TseFI	GTSAC	6 cut(s)	46, 355, 473, 598, 2768, 3197
Tsp45I	GTSAC	6 cut(s)	46, 355, 473, 598, 2768, 3197
TspGWI	ACGGA	3 cut(s)	225, 1605, 2283
TspRI	CASTG	7 cut(s)	1445, 2264, 2664, 2745, 3102, 3160, 3209
Tth111I	GACNNNGTC	1 cut(s)	3533
Van91I	CCANNNNNTGG	2 cut(s)	1605, 2624
Vha464I	CTTAAG	2 cut(s)	1336, 1991
VpaK11BI	GGWCC	2 cut(s)	1596, 2788
VspI	ATTAAT	1 cut(s)	453
XagI	CCTNNNNNAGG	3 cut(s)	336, 915, 2514
XapI	RAATTY	5 cut(s)	325, 536, 727, 967, 1805
XceI	RCATGY	5 cut(s)	1163, 2011, 2564, 3222, 4093
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3209
XmiI	GTMKAC	1 cut(s)	2263
XmnI	GAANNNNTTC	2 cut(s)	2586, 2848
ZrmI	AGTACT	2 cut(s)	932, 1501
Zsp2I	ATGCAT	4 cut(s)	1028, 1767, 3520, 3992

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.