Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0008066

Physical Location & Seq

Genomic Coordinates Reverse (-)

471179 .. 480026

Gene Structure 8848 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG008100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4917 bp

ATGGAATCACCTGTTGGAGGTGTGAGGCAAACATCTGTGATAGTTGTTACATTGGAGACTGGTGAAGTATATGTAATTGCGAGTTTGTCGTCTAGGCTTGATACTCAGGTCATATATGTTGATCCTACTACTGGAGCACTTCGCTACAATGCGAAGCCAGGATTTGATGTTTTTAAATCCGAAAAGGAAGCTTTGAATTACATTACAAACGGTTCGGAGTGGTTGTGTAGGAGCACTACCTATGCCCGTGCAATATTGGGCTATGCTGCTTTGGGCAGCTTTGGTTTGCTGCTTGTGGCTACCAAGTTGACTGCTACTGTTCCGAATCTGCCTGGCGGAGGGTCTGTGTATACAGTGACTGAGAGCCAATGGATCAAGATATTGCTTCAGAATCCACAGCCCCAGGGGAAAGGAGAAGTGAAGAATGTTAATGAATTGACTGATATGGACATTGATGGGAAGCATTACTTTTGTGAGGCAAGGGACATCACTAGGCCATTTCCTAGCCGTATGTGCTTGCGTGAACCTGACAATGAATTCGTCTGGAATGCTTGGTTCTCGATGCCTTTCAAAAACATTGGGCTTCCACACCATTGTGTCACTCTTCTTCAGGGTTTTGCAGAATGTCGAAGTTTTGGATCCTCGGGGAATCTGGAAGGAGTTGTTGCTCTCATAGCTCGTCGTAGCAGGCTGCATCCTGGTACTCGATACTTGGCTAGGGGATTAAATTCATGTTATAGCACAGGAAACGAAGTGGAGTGTGAGCAACTTGTATGGGTCCCTAGAAGGGTTGGCCAAACTGTTCCTTTCAACACATATGTCTGGCGGCGGGGGACAATTCCAATCTGGTGGGGTGCAGAGTTAAAGATCACTGCTGCTGAAGCAGAAATATATGTTTCAGACCGCGATCCTTATAAAGGGAGCGCAGATTACTACCAGAGGTTGAGTAAGAGATATGATGCACGGAATTTAGATGTAACTGTTGGGGGGACTCAGAATAGAACAGCATCAGTTCCAATTGTATGTATCAACTTGCTTAGAAATGGAGAAGGAAAGTCGGAATCCATTTTAGTACAGCATTTTGAGGAATCTTTAAACTACGTCAGATCCACAGGAAAACTTCCTTATACGCGAATTCATCTAATAAATTATGACTGGCATGCAAGTATAAAGTTAAAAGGCGAACAGCAAACCATTGAAGGATTATGGAGACATCTAAAAGCACACACTGTATCTATAGCCATTTCTGAAGGAGATTATTTGCCTTCACGAGACAGAATTAAGGACTGCAGGGGAGAAATTATCTACAATGATGACTTTGAAGGTGCCTTCTGCTTGAGATCCCATCAAAATGGTGTAATACGTTTCAACTGTGCTGATTCTTTGGATAGAACAAATGCTGCAAGTTATTTTGGCTCTCTCCAGGTTTTTGTGGAGCAGTGTAGGCGGCTTGGTATATCGCTTGACAGTGATTTGGCATTTGGGTATCAGTCAATGACTAATTATGCCGGATATACAGCTCCTCTGCCACCAGGCTGGGAGAAGAGGTCGGATGCAGTAACAGGGAAAACATACTACATTGATCACAATACTAGGACCACAACATGGATGCATCCATGTCCCGATAAACCTTGGAAAAGATTTGATATGTCATTTGAGGATTTCAAAAGGTCAACAATTTTATCTCCAGTATCCACGCTTGCAGATCTTTTTCTGCTTGCTGGTGATATTCATGCAACACTTTATACTGGCTCTAAAGCTATGCATAGTCAAATCCTCAGCATTTTCAATGAAGATGCTGGAAAGTTTAAACAGTTTTCTGCAGCACAGAATATGAAAATAACTTTGCAGAGGCGGTATAAAAATGCAGTTGTAGACAGCTCTCGTCAAAAGCAACTGGAAATGTTCCTTGGAATGAGATTATTCAAGCACCTTCCATCAGTTTCTTTTCACCCTCTAAATGTTGTCTCTCGACCATCTGGTTTCTTCCTTAAGCCAGTGGCTAACATGTTTCCAGGTTCCAATGGTGAAGCCAGTCTTCTGAGTTTCAAAAGAAAAGACCTAATCTGGGTTTGCCCACAAGCTGCAGATGTGGTTGAACTTTTTATCTATCTAGGTGAGCCTTGCCATGTATGTCAGCTTCTTCTCACAGTATCCCATGGTGCAGATGACTCGACTTATCCATCAACAGTTGATGTAAGGATGGGGCGCTGTTTAGATGGGCTAAAACTGGTCTTGGAGGGTGCTTCGATCCCTCACTGTGTAAATGGAACAAATCTCGTGATACCCATACCGGGGCCAATTAGTCCAGAGGATATGGCTATAACAGGAGCTGGTGCACGCCTTCATGCTCAAGATATATCTACCTTTCCATTATTGTATGATTTTGAAGAATTGGAAGGAGAACTTGACTTCCTTACTCGTGTAGTTGCCCTTACATTTTATCCCGCTGTCTCTGGAAGAACCCCTATCACTCTTGGTGAGATAGAAGTCCTTGGAGTTTCTCTTCCTTGGAAGGGTGTATTTAATAAAGAAGGCCGTGGTGCAAGATTACCTGAACAGGCTAAAAACTTTCAGAATGAAACCAAACCATCTTTTTCTCGTTCAGATACCAATCCATTTTCTGGTGCTTCATCTAATGATGTGCCACCACCAGTGCAACCAAGTGTGTCCGCAAACAATTTGGTTGACCTGTTGACTGGAGAGGTCATCCTTTCAGAACAGATTGCTCAGCCAGTGATAGGAAATGCTGTGGATAAGGGAGGTGACTTGCTTGACTTTTTAGACCAAGCTGTTGTTGAATATCATGGTGCTCAAAATGATCATAAATTGTCTTCATCACACGATGGTAGATCTTCAGATAGCAGTTCCCAGCAGTACATTGATCGATTGAAATCACTTACTGGGCCACGTATGGAAAGAAAACTAGACTTCATGGAAGCTATGAAACTTGAAATTGAGCGACTCCGGCTGAATATTTCTGCTGCTGAAAGGGATAGAGCTTTATTATCAATTGGAACCGACCCTGCTACCATAAATCCTAATGTGTTACTTGATGAACGTTACATGGGAAGATTGTGCAGAGTTGCAAACTCCCTTGCTCTTCTTGGACAAGCGTCCTTGGAAGACAGAATTACATCTGCTATTGGTCTTGAGACTACTGATGACAATGTGATAGATTTTTGGAATATAAGTAAAATTGGGGAGTGTTGTTACGGGGGCATGTGTGAGGTGCGTGCTGAAACTGATCCGCGTACAAGTAAATCCTTTTCAGAATCATCAGGTGGAGGTTCTCCTTCGATCTTGTTATGTTCTCAATGTCAAAGGAAAGTTTGTAAAGTTTGTTGTGCTGGGAGAGGAGCCCTTCTGGTTTCAGGCTATGGCTCAAGGGAGGCCATGAATTACAATGGTGTGGTAAGGCAGGGGGGATCAAGCCATGGTAGCCAGGTTGATATCTCTACAAATCGTTCAGTAGTGCTTGATGGTGTTGTTTGCAAACGATGCTGCCATGAGATTGTGCTTGATGCTTTGATATTGGACTACGTTAGGGTTTTGTTAAGCATGAGGAGAAGTTCCCGTGCAGATGCTGCTGCGCATGAAGCCTTGAACCAGGTGACTGGATTTTCCTTAAAAGATGGTCTTTCTGAGAGTAATCAGTCTTCTGGAAAGAGATCCATTAAATCTCTGCGACAAGTACTTGACGGAGAGGAATCCCTTGCTGAGTTTCCATTCGCCAGCTTCTTACACTCGGTTGAAACGGCTACTGATTCAGCACCACTCTTGTCATTGCTTGCTCCTCTTGATTGTGGATCACGGCATTCTTACTGGAAAGCTCCTCCTAGTACAACCTCTGTTGAATTCATTATTGTTCTTGGTACCCTTTCTGATGTCAGTGGGGTTTCTTTGCTTATTAGTCCATGTGGTTACTCCGAGGCTGAAGCTCCCACCGTGCAAATCTGGGCCAGCAATAAAATACACAAGGAAGAAAGGTCATGCATGGGAAAATGGGATGTGCAGTCCCTGATCGCGTCTTCCTCTGAATATTTTGGACCAGAAAAATTCGTTAGAGAAGATCAAGTGCCTAGGCATGTGAAGTTTGCATTCAAGAATCCAGTTCGGTGTCGCATTATTTGGGTTACCTTACGTCTTCAACGACCTGGTTCAAGTTCTCTTAATTTTGAGAACTTGAATCTGTTGTCTCTTGATGAAAATCCATTTGCAGAAGTAACTCGGCGTGCCTCTTTCGGAGGAGCAGTTGAAAGAGAGCCGTGTCTTCATGCAAAAAGGATACTGGTAGTAGGAAGCCCAGTGAAAAAAGATCTGGCACGCACATCTTCACAAGGCTCTGATCAGATGAATATGAAAAACTGGGTGGAGAGAGATCCACAATTAAATAGATTTAGGGTTCCAATTGAGGCTGAGAGGTTGTTGGATAATGATATAGTCTTGGAACAGTTTCTATCTCCTGCTTCACCTTTGCTTGCTGGGTTTCGTCTTGATGCTTTTGGTGCAATAAAACCTTTGGTTACCCATTCGCCCTCTTCAAATGCACGCATTTGGGATGTGTCAGCCACACTTTTAGATGACAGGCACATCTCTCCTGCTGTGCTGTATATACAAGTATCTATTTTCCAGGAACCCAACAACATGGTGACCGTCGCAGAATATAGGTTGCCAGAGGCTAAGCCTGGAACTGCTATGTACTTTGATTTCCCTAGAAAGATACAAACCCGCAGAATTACATTTAAACTTCTTGGAGATGTTACAGCATTTGCAGATGATCCAACAGAACAGGATGATCCAGGTTCCAGAGGTCTACCAGTAGCAGCCGGATTGTCATTAGCCAATAGAATTAAGTTGTATTACTATGATGATCCCTACGAACTTGGAAAATGGGCGAGCCTTTCAGCAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0031348 GO:0033612 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0060548 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Syja_N WW

Protein Analysis

1638

Amino Acids

181.38

Weight (kDa)

6.36

Isoelectric Point (pI)

42.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syja_N	PF02383	84 - 390	1.6e-39	SacI homology domain
WW	PF00397	509 - 539	1.1e-11	WW domain
SAC9_GBDL_1st	PF24790	653 - 838	2e-97	SAC9 first GBDL domain
SAC9_C8D	PF24791	1066 - 1176	6.2e-21	SAC9 C8D domain
SAC9_GBDL_2nd	PF24789	1236 - 1435	5.9e-101	SAC9 second GBDL domain
SAC9_C	PF24765	1463 - 1638	7.1e-91	SAC9 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59770 AT3G59770 AT3G59770 AT3G59770
fragaria_vesca	FvH4_1g00530 FvH4_1g00530
malus_domestica	MD02G1004400.v1.1 MD15G1148100.v1.1
prunus_persica	Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1
pyrus_communis	pycom02g00290 pycom15g13240
rosa_chinensis	RchiOBHm_Chr2g0085161
rosa_laevigata	RLG00000015661
rosa_multiflora	Rmu_sc0008647.1_g000009
rosa_roxburghii	Rroxscaffold_2G00155600
rosa_rugosa	Rorug01G0458000
rosa_samantha	Rh2AG006300 Rh2BG007100 Rh2CG007900 Rh2DG008100
rosa_wichuraiana	Rw2G000600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	915
Acc16I	TGCGCA	1 cut(s)	3594
Acc65I	GGTACC	1 cut(s)	3875
AccB1I	GGYRCC	2 cut(s)	1325, 3875
AccB7I	CCANNNNNTGG	2 cut(s)	1605, 2624
AccI	GTMKAC	3 cut(s)	350, 1875, 4816
AccII	CGCG	4 cut(s)	906, 1132, 3253, 4028
AclI	AACGTT	1 cut(s)	3061
AcoI	YGGCCR	1 cut(s)	793
AcsI	RAATTY	6 cut(s)	536, 727, 967, 1134, 3857, 4058
AcuI	CTGAAG	6 cut(s)	371, 593, 900, 1269, 2841, 3957
AfaI	GTAC	8 cut(s)	703, 1074, 2879, 3256, 3696, 3844, 3877, 4703
AflII	CTTAAG	1 cut(s)	1991
AflIII	ACRYGT	1 cut(s)	2007
AjuI	GAANNNNNNNTTGG	3 cut(s)	29, 1893, 1925
AleI	CACNNNNGTG	1 cut(s)	594
AloI	GAACNNNNNNTCC	2 cut(s)	2397, 2429
Alw21I	GWGCWC	4 cut(s)	139, 236, 2341, 2815
Alw26I	GTCTC	7 cut(s)	50, 1204, 1266, 1972, 2458, 3149, 4203
Alw44I	GTGCAC	1 cut(s)	2337
AlwNI	CAGNNNCTG	4 cut(s)	359, 2333, 3587, 4021
Ama87I	CYCGRG	1 cut(s)	643
AoxI	GGCC	7 cut(s)	494, 793, 2297, 2536, 2906, 3393, 3960
ApaLI	GTGCAC	1 cut(s)	2337
ApoI	RAATTY	6 cut(s)	536, 727, 967, 1134, 3857, 4058
ArsI	GACNNNNNNTTYG	2 cut(s)	521, 553
Asp700I	GAANNNNTTC	1 cut(s)	4455
Asp718I	GGTACC	1 cut(s)	3875
AspA2I	CCTAGG	1 cut(s)	4082
AspLEI	GCGC	3 cut(s)	926, 2211, 3595
AspS9I	GGNCC	6 cut(s)	778, 1596, 2297, 2906, 3960, 4049
AsuC2I	CCSGG	1 cut(s)	2295
AvaI	CYCGRG	1 cut(s)	643
AvaII	GGWCC	3 cut(s)	778, 1596, 4049
AvrII	CCTAGG	1 cut(s)	4082
BaeGI	GKGCMC	1 cut(s)	2341
BaeI	ACNNNNGTAYC	2 cut(s)	93, 126
BalI	TGGCCA	1 cut(s)	795
BamHI	GGATCC	1 cut(s)	638
BanI	GGYRCC	2 cut(s)	1325, 3875
BanII	GRGCYC	1 cut(s)	3364
BarI	GAAGNNNNNNTAC	4 cut(s)	2124, 2156, 3643, 3675
BauI	CACGAG	3 cut(s)	1269, 2279, 2421
BbsI	GAAGAC	7 cut(s)	2030, 2826, 3132, 3651, 4023, 4139, 4265
Bbv12I	GWGCWC	4 cut(s)	139, 236, 2341, 2815
BbvCI	CCTCAGC	1 cut(s)	1778
BceAI	ACGGC	5 cut(s)	492, 2523, 3774, 3830, 4252
BcgI	CGANNNNNNTGC	2 cut(s)	2154, 2188
BciVI	GTATCC	3 cut(s)	1702, 2164, 4281
BclI	TGATCA	3 cut(s)	1582, 2821, 4348
BcnI	CCSGG	1 cut(s)	2295
BcoDI	GTCTC	7 cut(s)	50, 1204, 1266, 1972, 2458, 3149, 4203
BfmI	CTRYAG	4 cut(s)	1236, 1288, 1821, 2085
BfoI	RGCGCY	1 cut(s)	2212
BfrI	CTTAAG	1 cut(s)	1991
BfuI	GTATCC	3 cut(s)	1702, 2164, 4281
BglII	AGATCT	3 cut(s)	1705, 2852, 4318
BlnI	CCTAGG	1 cut(s)	4082
BlpI	GCTNAGC	2 cut(s)	2730, 4683
BmcAI	AGTACT	1 cut(s)	3696
Bme18I	GGWCC	3 cut(s)	778, 1596, 4049
BmeT110I	CYCGRG	1 cut(s)	643
BmgT120I	GGNCC	6 cut(s)	778, 1596, 2297, 2906, 3960, 4049
BmrI	ACTGGG	3 cut(s)	2913, 4301, 4378
BmuI	ACTGGG	3 cut(s)	2913, 4301, 4378
BoxI	GACNNNNGTC	1 cut(s)	3115
BpiI	GAAGAC	7 cut(s)	2030, 2826, 3132, 3651, 4023, 4139, 4265
BpmI	CTGGAG	4 cut(s)	153, 1406, 1671, 2721
Bpu10I	CCTNAGC	1 cut(s)	1778
Bpu1102I	GCTNAGC	2 cut(s)	2730, 4683
BpuEI	CTTGAG	4 cut(s)	1357, 2337, 3173, 3370
BpuMI	CCSGG	1 cut(s)	2295
Bsa29I	ATCGAT	1 cut(s)	2887
BsaAI	YACGTR	1 cut(s)	2912
BsaBI	GATNNNNATC	3 cut(s)	2613, 2893, 4209
BsaXI	ACNNNNNCTCC	8 cut(s)	209, 239, 330, 360, 1532, 1562, 4239, 4269
Bse3DI	GCAATG	1 cut(s)	3785
Bse8I	GATNNNNATC	3 cut(s)	2613, 2893, 4209
BseCI	ATCGAT	1 cut(s)	2887
BseGI	GGATG	9 cut(s)	694, 1558, 1612, 1614, 2208, 2709, 4015, 4567, 4801
BseJI	GATNNNNATC	3 cut(s)	2613, 2893, 4209
BseMI	GCAATG	1 cut(s)	3785
BseMII	CTCAG	9 cut(s)	119, 351, 1007, 1792, 2033, 2744, 3636, 3711, 4410
BseRI	GAGGAG	6 cut(s)	1512, 3372, 3580, 3786, 3825, 4263
BseSI	GKGCMC	1 cut(s)	2341
BseYI	CCCAGC	4 cut(s)	1536, 2871, 3350, 4484
BsgI	GTGCAG	5 cut(s)	876, 2184, 3100, 3600, 4034
Bsh1236I	CGCG	4 cut(s)	906, 1132, 3253, 4028
BshFI	GGCC	7 cut(s)	496, 795, 2299, 2538, 2908, 3395, 3962
BshNI	GGYRCC	2 cut(s)	1325, 3875
BshVI	ATCGAT	1 cut(s)	2887
BsiHKAI	GWGCWC	4 cut(s)	139, 236, 2341, 2815
BsiHKCI	CYCGRG	1 cut(s)	643
BsiSI	CCGG	4 cut(s)	1509, 2294, 2968, 4830
BslFI	GGGAC	6 cut(s)	497, 764, 847, 1003, 1605, 4003
BsmAI	GTCTC	7 cut(s)	50, 1204, 1266, 1972, 2458, 3149, 4203
BsmFI	GGGAC	6 cut(s)	497, 764, 847, 1003, 1605, 4003
BsmI	GAATGC	3 cut(s)	553, 3817, 4100
BsnI	GGCC	7 cut(s)	496, 795, 2299, 2538, 2908, 3395, 3962
BsoBI	CYCGRG	1 cut(s)	643
Bsp1286I	GDGCHC	5 cut(s)	139, 236, 2341, 2815, 3364
Bsp1720I	GCTNAGC	2 cut(s)	2730, 4683
Bsp19I	CCATGG	2 cut(s)	2158, 3436
BspANI	GGCC	7 cut(s)	496, 795, 2299, 2538, 2908, 3395, 3962
BspCNI	CTCAG	9 cut(s)	118, 352, 1006, 1791, 2034, 2743, 3637, 3712, 4411
BspDI	ATCGAT	1 cut(s)	2887
BspFNI	CGCG	4 cut(s)	906, 1132, 3253, 4028
BspMAI	CTGCAG	3 cut(s)	1292, 1825, 2089
BspQI	GCTCTTC	1 cut(s)	3108
BspT107I	GGYRCC	2 cut(s)	1325, 3875
BspTI	CTTAAG	1 cut(s)	1991
BsrDI	GCAATG	1 cut(s)	3785
BssNAI	GTATAC	1 cut(s)	351
BssSI	CACGAG	3 cut(s)	1269, 2279, 2421
BssT1I	CCWWGG	8 cut(s)	1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
Bst1107I	GTATAC	1 cut(s)	351
Bst2BI	CACGAG	3 cut(s)	1269, 2279, 2421
Bst6I	CTCTTC	5 cut(s)	609, 1538, 2511, 3108, 4546
BstAFI	CTTAAG	1 cut(s)	1991
BstAPI	GCANNNNNTGC	2 cut(s)	3501, 3587
BstBAI	YACGTR	1 cut(s)	2912
BstDSI	CCRYGG	3 cut(s)	2158, 2539, 3436
BstEII	GGTNACC	3 cut(s)	4135, 4525, 4651
BstENI	CCTNNNNNAGG	3 cut(s)	15, 336, 915
BstF5I	GGATG	9 cut(s)	694, 1558, 1612, 1614, 2208, 2709, 4015, 4567, 4801
BstFNI	CGCG	4 cut(s)	906, 1132, 3253, 4028
BstH2I	RGCGCY	1 cut(s)	2212
BstHHI	GCGC	3 cut(s)	926, 2211, 3595
BstMAI	GTCTC	7 cut(s)	50, 1204, 1266, 1972, 2458, 3149, 4203
BstNSI	RCATGY	4 cut(s)	1163, 2011, 3226, 4091
BstPAI	GACNNNNGTC	1 cut(s)	3115
BstPI	GGTNACC	3 cut(s)	4135, 4525, 4651
BstSFI	CTRYAG	4 cut(s)	1236, 1288, 1821, 2085
BstSLI	GKGCMC	1 cut(s)	2341
BstUI	CGCG	4 cut(s)	906, 1132, 3253, 4028
BstV2I	GAAGAC	7 cut(s)	2030, 2826, 3132, 3651, 4023, 4139, 4265
BstX2I	RGATCY	8 cut(s)	638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
BstXI	CCANNNNNNTGG	5 cut(s)	849, 1352, 1536, 3617, 4648
BstYI	RGATCY	8 cut(s)	638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
BstZ17I	GTATAC	1 cut(s)	351
Bsu15I	ATCGAT	1 cut(s)	2887
BsuI	GTATCC	3 cut(s)	1702, 2164, 4281
BsuRI	GGCC	7 cut(s)	496, 795, 2299, 2538, 2908, 3395, 3962
BsuTUI	ATCGAT	1 cut(s)	2887
BtgI	CCRYGG	3 cut(s)	2158, 2539, 3436
BtsCI	GGATG	9 cut(s)	694, 1558, 1612, 1614, 2208, 2709, 4015, 4567, 4801
BtsI	GCAGTG	2 cut(s)	870, 1445
CaiI	CAGNNNCTG	4 cut(s)	359, 2333, 3587, 4021
CfoI	GCGC	3 cut(s)	926, 2211, 3595
Cfr13I	GGNCC	6 cut(s)	778, 1596, 2297, 2906, 3960, 4049
ClaI	ATCGAT	1 cut(s)	2887
CseI	GACGC	2 cut(s)	3105, 4017
CsiI	ACCWGGT	2 cut(s)	3609, 4156
Csp6I	GTAC	8 cut(s)	702, 1073, 2878, 3255, 3695, 3843, 3876, 4702
CviQI	GTAC	8 cut(s)	702, 1073, 2878, 3255, 3695, 3843, 3876, 4702
DraI	TTTAAA	4 cut(s)	175, 1095, 1810, 4747
EaeI	YGGCCR	1 cut(s)	793
Eam1104I	CTCTTC	5 cut(s)	609, 1538, 2511, 3108, 4546
EarI	CTCTTC	5 cut(s)	609, 1538, 2511, 3108, 4546
EciI	GGCGGA	1 cut(s)	351
Eco130I	CCWWGG	8 cut(s)	1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
Eco24I	GRGCYC	1 cut(s)	3364
Eco32I	GATATC	1 cut(s)	3454
Eco47I	GGWCC	3 cut(s)	778, 1596, 4049
Eco57I	CTGAAG	6 cut(s)	371, 593, 900, 1269, 2841, 3957
Eco88I	CYCGRG	1 cut(s)	643
Eco91I	GGTNACC	3 cut(s)	4135, 4525, 4651
EcoNI	CCTNNNNNAGG	3 cut(s)	15, 336, 915
EcoO109I	RGGNCCY	1 cut(s)	778
EcoO65I	GGTNACC	3 cut(s)	4135, 4525, 4651
EcoRI	GAATTC	3 cut(s)	536, 1134, 3857
EcoRV	GATATC	1 cut(s)	3454
EcoT14I	CCWWGG	8 cut(s)	1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
EcoT22I	ATGCAT	3 cut(s)	1614, 1767, 3998
EcoT38I	GRGCYC	1 cut(s)	3364
ErhI	CCWWGG	8 cut(s)	1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
FalI	AAGNNNNNCTT	8 cut(s)	452, 484, 2022, 2054, 2391, 2423, 3624, 3656
FaqI	GGGAC	6 cut(s)	497, 764, 847, 1003, 1605, 4003
FauI	CCCGC	3 cut(s)	822, 2455, 4739
FauNDI	CATATG	1 cut(s)	817
FbaI	TGATCA	3 cut(s)	1582, 2821, 4348
FblI	GTMKAC	3 cut(s)	350, 1875, 4816
FokI	GGATG	9 cut(s)	681, 1565, 1599, 1621, 2215, 2696, 4022, 4574, 4808
FriOI	GRGCYC	1 cut(s)	3364
FspI	TGCGCA	1 cut(s)	3594
GlaI	GCGC	3 cut(s)	925, 2210, 3594
GsaI	CCCAGC	4 cut(s)	1540, 2875, 3354, 4488
GsuI	CTGGAG	4 cut(s)	153, 1406, 1671, 2721
HaeII	RGCGCY	1 cut(s)	2212
HaeIII	GGCC	7 cut(s)	496, 795, 2299, 2538, 2908, 3395, 3962
HapII	CCGG	4 cut(s)	1509, 2294, 2968, 4830
HgaI	GACGC	2 cut(s)	3105, 4017
HhaI	GCGC	3 cut(s)	926, 2211, 3595
Hin6I	GCGC	3 cut(s)	924, 2209, 3593
HinP1I	GCGC	3 cut(s)	924, 2209, 3593
HincII	GTYRAC	4 cut(s)	309, 1674, 2689, 2697
HindII	GTYRAC	4 cut(s)	309, 1674, 2689, 2697
HindIII	AAGCTT	1 cut(s)	189
HpaII	CCGG	4 cut(s)	1509, 2294, 2968, 4830
Hpy166II	GTNNAC	9 cut(s)	309, 351, 524, 1674, 1876, 2339, 2689, 2697, 4817
Hpy8I	GTNNAC	9 cut(s)	309, 351, 524, 1674, 1876, 2339, 2689, 2697, 4817
Hpy99I	CGWCG	2 cut(s)	684, 4661
HpyCH4IV	ACGT	6 cut(s)	1101, 1363, 2911, 3061, 3543, 4144
HpySE526I	ACGT	6 cut(s)	1101, 1363, 2911, 3061, 3543, 4144
HspAI	GCGC	3 cut(s)	924, 2209, 3593
KflI	GGGWCCC	1 cut(s)	778
KpnI	GGTACC	1 cut(s)	3879
Ksp22I	TGATCA	3 cut(s)	1582, 2821, 4348
LguI	GCTCTTC	1 cut(s)	3108
MabI	ACCWGGT	2 cut(s)	3609, 4156
MaeII	ACGT	6 cut(s)	1101, 1363, 2911, 3061, 3543, 4144
MfeI	CAATTG	3 cut(s)	1017, 3012, 4410
MflI	RGATCY	8 cut(s)	638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
MhlI	GDGCHC	5 cut(s)	139, 236, 2341, 2815, 3364
MlsI	TGGCCA	1 cut(s)	795
MluNI	TGGCCA	1 cut(s)	795
MlyI	GAGTC	3 cut(s)	985, 2165, 2958
MmeI	TCCRAC	4 cut(s)	1038, 1530, 4410, 4808
Mox20I	TGGCCA	1 cut(s)	795
Mph1103I	ATGCAT	3 cut(s)	1614, 1767, 3998
MroXI	GAANNNNTTC	1 cut(s)	4455
MscI	TGGCCA	1 cut(s)	795
MslI	CAYNNNNRTG	2 cut(s)	594, 1350
Msp20I	TGGCCA	1 cut(s)	795
MspA1I	CMGCKG	1 cut(s)	2450
MspCI	CTTAAG	1 cut(s)	1991
MspI	CCGG	4 cut(s)	1509, 2294, 2968, 4830
MssI	GTTTAAAC	1 cut(s)	1810
MunI	CAATTG	3 cut(s)	1017, 3012, 4410
Mva1269I	GAATGC	3 cut(s)	553, 3817, 4100
MvnI	CGCG	4 cut(s)	906, 1132, 3253, 4028
NciI	CCSGG	1 cut(s)	2295
NcoI	CCATGG	2 cut(s)	2158, 3436
NdeI	CATATG	1 cut(s)	817
NmeAIII	GCCGAG	1 cut(s)	4210
NmuCI	GTSAC	5 cut(s)	355, 598, 2765, 3613, 4651
NsbI	TGCGCA	1 cut(s)	3594
NsiI	ATGCAT	3 cut(s)	1614, 1767, 3998
NspI	RCATGY	4 cut(s)	1163, 2011, 3226, 4091
OliI	CACNNNNGTG	1 cut(s)	594
PaeI	GCATGC	1 cut(s)	1163
PasI	CCCWGGG	1 cut(s)	403
PciI	ACATGT	1 cut(s)	2007
PciSI	GCTCTTC	1 cut(s)	3108
PcsI	WCGNNNNNNNCGW	1 cut(s)	4150
PctI	GAATGC	3 cut(s)	553, 3817, 4100
PdmI	GAANNNNTTC	1 cut(s)	4455
PflMI	CCANNNNNTGG	2 cut(s)	1605, 2624
PfoI	TCCNGGA	1 cut(s)	4632
PleI	GAGTC	3 cut(s)	985, 2165, 2958
PmeI	GTTTAAAC	1 cut(s)	1810
PpsI	GAGTC	3 cut(s)	985, 2165, 2958
Ppu21I	YACGTR	1 cut(s)	2912
PpuMI	RGGWCCY	1 cut(s)	778
PscI	ACATGT	1 cut(s)	2007
PshAI	GACNNNNGTC	1 cut(s)	3115
PsiI	TTATAA	1 cut(s)	915
Psp1406I	AACGTT	1 cut(s)	3061
Psp5II	RGGWCCY	1 cut(s)	778
PspEI	GGTNACC	3 cut(s)	4135, 4525, 4651
PspFI	CCCAGC	4 cut(s)	1536, 2871, 3350, 4484
PspPI	GGNCC	6 cut(s)	778, 1596, 2297, 2906, 3960, 4049
PspPPI	RGGWCCY	1 cut(s)	778
PstI	CTGCAG	3 cut(s)	1292, 1825, 2089
PstNI	CAGNNNCTG	4 cut(s)	359, 2333, 3587, 4021
PsuI	RGATCY	8 cut(s)	638, 1106, 1340, 1705, 2852, 3671, 4318, 4381
RsaI	GTAC	8 cut(s)	703, 1074, 2879, 3256, 3696, 3844, 3877, 4703
RsaNI	GTAC	8 cut(s)	702, 1073, 2878, 3255, 3695, 3843, 3876, 4702
RseI	CAYNNNNRTG	2 cut(s)	594, 1350
SapI	GCTCTTC	1 cut(s)	3108
Sau96I	GGNCC	6 cut(s)	778, 1596, 2297, 2906, 3960, 4049
ScaI	AGTACT	1 cut(s)	3696
SchI	GAGTC	3 cut(s)	985, 2165, 2958
SduI	GDGCHC	5 cut(s)	139, 236, 2341, 2815, 3364
SexAI	ACCWGGT	2 cut(s)	3609, 4156
SfcI	CTRYAG	4 cut(s)	1236, 1288, 1821, 2085
SinI	GGWCC	3 cut(s)	778, 1596, 4049
SmiMI	CAYNNNNRTG	2 cut(s)	594, 1350
SmlI	CTYRAG	5 cut(s)	1336, 1991, 2352, 3152, 3385
SmoI	CTYRAG	5 cut(s)	1336, 1991, 2352, 3152, 3385
SphI	GCATGC	1 cut(s)	1163
SspI	AATATT	3 cut(s)	255, 2977, 4043
StyI	CCWWGG	8 cut(s)	1631, 1909, 2158, 2494, 2510, 3120, 3436, 4082
TaiI	ACGT	6 cut(s)	1104, 1366, 2914, 3064, 3546, 4147
TaqI	TCGA	8 cut(s)	560, 628, 706, 1972, 2174, 2249, 2887, 3299
TatI	WGTACW	5 cut(s)	1072, 2877, 3694, 3842, 4701
TauI	GCSGC	2 cut(s)	829, 1450
TseFI	GTSAC	5 cut(s)	355, 598, 2765, 3613, 4651
Tsp45I	GTSAC	5 cut(s)	355, 598, 2765, 3613, 4651
TspGWI	ACGGA	2 cut(s)	979, 3717
Van91I	CCANNNNNTGG	2 cut(s)	1605, 2624
Vha464I	CTTAAG	1 cut(s)	1991
VneI	GTGCAC	1 cut(s)	2337
VpaK11BI	GGWCC	3 cut(s)	778, 1596, 4049
XagI	CCTNNNNNAGG	3 cut(s)	15, 336, 915
XapI	RAATTY	6 cut(s)	536, 727, 967, 1134, 3857, 4058
XceI	RCATGY	4 cut(s)	1163, 2011, 3226, 4091
XmaJI	CCTAGG	1 cut(s)	4082
XmiI	GTMKAC	3 cut(s)	350, 1875, 4816
XmnI	GAANNNNTTC	1 cut(s)	4455
ZrmI	AGTACT	1 cut(s)	3696
Zsp2I	ATGCAT	3 cut(s)	1614, 1767, 3998

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.