Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008066

Physical Location & Seq

Genomic Coordinates Reverse (-)

10960421 .. 10969078

Gene Structure 8658 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1148100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4911 bp

ATGGAATCAGCTGGTGGTGGTATAAGGGAGACATCTGTTATAGTTGTGACATTGGATACTGGTGAAGTATATATAATTGCGAGTTTGTCTTCTAGGCTCGACACTCAGGTCATACATGTTGATCCCACAACCGGAGTGCTTCGCTACAATGCGAAGCCAGGATTTGATGTTTTCAAATCCGAAAAGGAAGCATTGGATTACATTACAGACGGATCACACTGGTCGTGTAGGAGTACAACCTATGCACATGCAATATTGGGTTATGCTGCTTTGGGCAGCATTGGTTTGCTTCTCGTGGCCACCAAGTTGACTGCTAGTGTTCCGAATTTGCCTGGTGGAGGGTGCATTTATACGGTGACTGAGAGCCAATGGATCAAGATTCAACTTCAGAATCCACAACCACAAGGGAAAGGAGAAGAAAAGAATGTTAATGAATTAACTGATCTTGACATTAATGGGAAGCACTACTTTTGTGAGGCAAGGGATATCACTCGACCATTTCCTAGCCGTATGAGCTTGCGTGAGCCTGATGATGAATTTGTTTGGAACGCCTGGTTCTCGATGCCTTTCAAAAACATTGGGTTGTCGCAGCATTGTGTCACTCTTCTGCAGGGTTTTGCGGAGTGTCGAACTTTTGGAACCTTAGGGAAGGTGGACGGAATTGTTGCTCTCATAGCTCGTCGTAGTAGGTTGCATCCTGGTACTCGGTACTTGGCTAGGGGATTGAATTCTTGTTTTAGCACAGGAAATGAAGTGGAGTGTGAGCAACTTGCATGGGTCCCTAGAAGGGCTGGTCAAACTGTTCCTTTTAACACATACGTGTGGCGACGCGGCACAATTCCAATTTGGTGGGGTGCAGAGTTAAAGATTACTGCTGCAGAAGCAGAAATATACGTTTCGGATAGTGATCCTTATAAAGGAAGTGCGGAGTACTACCAGAGGTTGAGTAAGAGGTATGATGCACGAAATTTTGATGTAGCCTTTGGGGGGAGTCGGAATAGAAAAGCGTTTGTTCCAATTGTTTGCATCAACTTGCTTAGGAGTGGAGAAGGAAAGTCAGAATGTATTTTAGTTCAACATTTTGAGGAATCTTTAAACTACATCAAGTCAACTGGAAAACTTCCTTATACTCGAATTCACTTGATAAATTATGACTGGCATGCCAGTATAAAGTTAAAAGGTGAACAGCAAACCATCGAAGGATTATGGAAACATCTAAAAGCACACACTGTTTCCATAGGCATTTCTGAGGGAGATTTTTTGCCTTCGCGGGAAAGAATTAAGGACTGCAGAGGAGAAATTATCTACAATGATGACTTTGAAGGCGCCTTCTGCTTAAGATCACGTCAAAATGGTGTGATACGTTTCAACTGTGCTGATTCTTTGGATAGGACAAATGCTGCAAGTTATTTTGGCTCCCTCCAGGTTTTCGTGGAGCAGTGTAGGCGGCTTTTTATATCACTTGATAGTGATTTGGCATTTGGTTATCAGTCAATGAGTAATTATGGTGGCTATACTGCTCCTTTGCCACCAGGCTGGGAGAAGAGATCTGATGCAGTAACAGGGAAAACTTTTTATGTTGATCACAACACTAGGACCACAACATGGATGCATCCGTGTCCCGATAAACCGTGGAAGAGATTTGATATGGCATTTGAGGAGTTTAAGAGGTCAACAATTTTATCACCGGTATCCCAGCTTGCTGATATTTTTCTGCTTGCGGGTGATATTCATGCAACACTTTATACTGGTTCGAAAGCTATGCATAGCCAAATCCTTAGCATCTTCAATGACGATGCAGGAAAATTCAAACAATTTTCTGCAGCACAAAATATGAAAATTACTTTGCAGCGGAGATATAAAAATGCAGTTGTAGACAGCTCTCGTCAAAAGCAACTGGAGATATTTCTTGGAATGAGACTATTCAAGCATCTTCCATCAGTTTCTTTTCACCCTCTTAATGTCGTCTCTCGACCATCTGGTTTCTTCCTTAAGCCAGTTGCTAACATGTTTCCAAGTTCCAATGGTGGAGCCAGTCTTCTGAGTTTCAAGAGAAAGGATCTAGTCTGGGTTTGCCCACAGGCCGCAGATGTTGTCGAACTTTTTATCTATTTAGGTGAACCTTGCCATGTTTGTCAGCTTCTTCTCACAATATCCCATGGTGTAGATGATTCAACTTATCCATCAACAGTTGACGTAAGGACAGGACGCTCATTAGATGGGCTAAAACTTGTCTTGGAGGGTGCTTCGATACCTCATTGTGTAAATGGAACAAACCTCTTGATACCCTTAACAGGGGCAATTAGTCCAGAGGATATGGCTGTAACAGGAGCTGGTTCACGTCTTCATGCTCAAGATACATCTAGCCTTCCATTATTGTATGATTTTGAAGAACTGGAAGGAGAACTTGACTTCCTTACTCGTGTAGTTGCCCTTACATTTTATCCTGCCGTCTCTGGAAAGAGCCCTATCACTTTTGGTGAGATAGAAGTCCTTGGAGTTTCTCTTCCTTGGAAGGGTGTATTTACTAACGAAGGCCCTGGTGCAAGTTTACCTGAACAAGCTAAAAACCTTCAGAATGAAAACAATCTTTTTTCCACTGGGTCAAAAACCAATCCATTTTCCGGTGCTTCATCTAATGAAAATGTGACGGTACCAGTGCAACCAAGTGCATCTGCCAACAATTTGGTTGATCTATTGACTGGAGAGGTTGTGCTTTCAGAACACTTTGCTGCACCAGTGATAGGAAATGCTGAGGACAAGGGAGGTGACTTGCTTGATTTCTTGGACCAAGCTATTGTTGAATATCATGGTGCTGAAACTGATCATAAATTACACGATGGAAAACCTTTAGATAGCAGTTCTCAGCAGTACATTGATTGTTTGAAATCCATTGCTGGGCCACGTATGGAAAAAAAACTAAACTTCATGGAAGCCATGAAACTTGAAATTGAACGCCTTCGGCTGGACATTTCTGCTGCTGAAAGGGATAGAGCTTTGTTATCTATAGGAACTGATCCTGCTACTATAAACCCTAATGTTTTACTTGATGAACGATACATGGGAAGATTGTGTAGAGTTGCAAACTCCGTTGCACTGCTTGGACAAGCATCCTTGGAAGACAAAATTACATCTGCTGTTGGTCTTGAGACTACTGATGATACTGCAATAGATTTTTGGAATATAACTAGCTTTGGTGAGCGCTGTTATGGCGGCACGTGTGAGGTGCGTGCTGAAACTAATGCACCTACACGTGCTTCCTTTTCAGAATCATCAGGGGTAGTTTCCCCATCTCTTTTCTTATGTTCCCAATGTGAAAGGAAAGTTTGTAAAGTTTGTTGTGCTGGGAGAGGGGCCCTTCTGGTTGCAGGCTATGGCTCAAGGGAGGCCATGAGTTACAATGGTGTGGTAAATCAGGGGGGGTCAGGCCATGGTTTCCAGGTTGATGTCTCAACAAATAATACAGTAGTGCTGGATAGTGTTATTTGTAAAAGATGCTGCGATGATACTGTGCTTGATGCATTGATCTTGGACTACGTCAGGGTCTTGGTAAGCAAGAGGAGAAGTGCCCGTGCAGATTCTGCTGCCCATGAGGCCTTGAACCAAGTGATTGGATATTCGGTAAGGAATTGTCTTTCTGAAAGGAATCAGTCTCCGGATAGACCGAGAACTATTAAAGTTCTGCAGAAATTACTTGGCAGTGTGGAATCGTTAGCTGAGTTTCCATTTGCCAGCTTTTTACACTCGGTTGAAACGGGAGCAGATTCAGCACCATTCTTGTCATTGCTTGCTCCTCTTGAATCTGGATCACGACATTCATACTGGAAAGCTCCTCCTAGTACCACTAGTGTTGAGTTTGTTATTGTTCTTGGCACCCTTTCTGATGTTAGTGGGGTTGTTTTGCTTATTAGTCCATGTGGTTATTCTGAGGCTGATGCTCCCACCGTGCAAATCTGGGCCAGCAATAAAATACACAAAGAAGAAAGGTCATGCATGGGAAAATGGGATTTGCAGTCCCAGATCACGTCTTCCTCAGAATATTATGGACCAGAAAAGTCGGTTAGAGAAAATGAAGTACCTAGGCATGTGAAGTTTGAATTTGGGAATCCAGTTCGATGCCGCATTATTTGGATAACATTACGCCTTCAACGACCTGGTTCAAAATCTCTTAATTTGGACAACTTGAATCTCTTGTCTCTTGATGAAAATCCATTTGCAGAAGTAACTCGGCGTGCCTCTTTTGGAGGAAAAGTTGAAAGAGAACCCTGTATTCATGCCAAAAGGATACTGGTAGTCGGAAGCTCGGTGAAAAAAGAGATGGTAGATACATCACAAGCCTCCGATCCGATGAATCTGAAAGGCTGGCTGGAGAGAAGTCCACCACTGAATAGATTTAGGGTTCCCATTGAGGCTGAGAGGCTGTTGGACCATGATATTATTTTGGAGCAATATCTTTCTCCTGCATCACCTTTGCTTGCCGGATTTCGTCTTGATGCTTTTGGTGCAATAAGGCCTCTGGTTACCCATTCACCCTTTTCAAATGCACACATCTGGGATACGTCAGCAACACTTGTAGACGAGAGACACATCTCTCCAGCCGTTCTGTATATACAAGTATCTGCTGTCCAGGAACCGCACAGCATCGTACCGATTGCCGAATATCGCTTGCCGGAGGCTAAGTCTGGAACAGCGATGTACTTTGATTTCCCTCGAGAGATACAAACCCGCAGAATTATGTTTAAACTGCTTGGAGATATTACAGCATTTGCCGATGACCCAACGGAGCAGGATGATGCCGGTTCCAGAGGTGTACCGGTGGCGGCAGGCTTGTCGTTGGCCAATAAAATAAAGTTGTATTACTACGCTGATCCGTACGAACTTGGAAAATGGGCAAGCCTCTCTGCCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0031348 GO:0033612 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0060548 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Syja_N WW

Protein Analysis

1637

Amino Acids

180.62

Weight (kDa)

6.06

Isoelectric Point (pI)

41.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syja_N	PF02383	83 - 390	9.3e-41	SacI homology domain
WW	PF00397	509 - 539	2.1e-11	WW domain
SAC9_GBDL_1st	PF24790	653 - 838	1.1e-97	SAC9 first GBDL domain
SAC9_C8D	PF24791	1063 - 1174	6.4e-19	SAC9 C8D domain
SAC9_GBDL_2nd	PF24789	1236 - 1434	3.2e-101	SAC9 second GBDL domain
SAC9_C	PF24765	1462 - 1636	9.4e-90	SAC9 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59770 AT3G59770 AT3G59770 AT3G59770
fragaria_vesca	FvH4_1g00530 FvH4_1g00530
malus_domestica	MD02G1004400.v1.1 MD15G1148100.v1.1
prunus_persica	Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1 Prupe.7G266700_v2.0.a1
pyrus_communis	pycom02g00290 pycom15g13240
rosa_chinensis	RchiOBHm_Chr2g0085161
rosa_laevigata	RLG00000015661
rosa_multiflora	Rmu_sc0008647.1_g000009
rosa_roxburghii	Rroxscaffold_2G00155600
rosa_rugosa	Rorug01G0458000
rosa_samantha	Rh2AG006300 Rh2BG007100 Rh2CG007900 Rh2DG008100
rosa_wichuraiana	Rw2G000600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	915
AasI	GACNNNNNNGTC	1 cut(s)	107
Acc65I	GGTACC	1 cut(s)	2653
AccB1I	GGYRCC	3 cut(s)	1325, 2653, 3872
AccB7I	CCANNNNNTGG	1 cut(s)	1605
AccI	GTMKAC	2 cut(s)	1875, 4575
AccII	CGCG	2 cut(s)	831, 1270
AccIII	TCCGGA	1 cut(s)	3655
AclWI	GGATC	9 cut(s)	116, 220, 380, 902, 2067, 3011, 3814, 4337, 4862
AcoI	YGGCCR	2 cut(s)	297, 4836
AcsI	RAATTY	7 cut(s)	325, 536, 727, 967, 1134, 1805, 4097
AcuI	CTGAAG	2 cut(s)	371, 2558
AcvI	CACGTG	2 cut(s)	3219, 3254
AcyI	GRCGYC	1 cut(s)	1326
AfeI	AGCGCT	1 cut(s)	3203
AflII	CTTAAG	2 cut(s)	1336, 1991
AflIII	ACRYGT	5 cut(s)	115, 819, 2007, 3218, 3251
AgeI	ACCGGT	2 cut(s)	1687, 4813
AhlI	ACTAGT	1 cut(s)	3845
AjiI	CACGTC	3 cut(s)	1346, 2342, 4026
AleI	CACNNNNGTG	1 cut(s)	818
AloI	GAACNNNNNNTCC	2 cut(s)	2397, 2429
Alw26I	GTCTC	9 cut(s)	23, 1912, 1972, 2458, 3143, 3454, 3657, 4200, 4576
AlwI	GGATC	9 cut(s)	116, 220, 380, 902, 2067, 3011, 3814, 4337, 4862
AlwNI	CAGNNNCTG	2 cut(s)	2333, 3581
Ama87I	CYCGRG	1 cut(s)	4710
Aor13HI	TCCGGA	1 cut(s)	3655
Aor51HI	AGCGCT	1 cut(s)	3203
ApaI	GGGCCC	1 cut(s)	3358
ApoI	RAATTY	7 cut(s)	325, 536, 727, 967, 1134, 1805, 4097
AseI	ATTAAT	1 cut(s)	453
AsiGI	ACCGGT	2 cut(s)	1687, 4813
Asp700I	GAANNNNTTC	1 cut(s)	2958
Asp718I	GGTACC	1 cut(s)	2653
AspA2I	CCTAGG	1 cut(s)	4079
AspLEI	GCGC	2 cut(s)	1328, 3204
AsuII	TTCGAA	1 cut(s)	1754
AvaI	CYCGRG	1 cut(s)	4710
AvaII	GGWCC	5 cut(s)	778, 1596, 2788, 4046, 4426
AvrII	CCTAGG	1 cut(s)	4079
AxyI	CCTNAGG	1 cut(s)	643
BaeGI	GKGCMC	2 cut(s)	3358, 3571
BalI	TGGCCA	2 cut(s)	299, 4838
BanI	GGYRCC	3 cut(s)	1325, 2653, 3872
BanII	GRGCYC	2 cut(s)	2468, 3358
BauI	CACGAG	2 cut(s)	293, 2421
BbrPI	CACGTG	2 cut(s)	3219, 3254
BbsI	GAAGAC	5 cut(s)	81, 2030, 2336, 3126, 4020
BbvCI	CCTCAGC	1 cut(s)	2754
BccI	CCATC	8 cut(s)	1202, 1945, 1984, 2191, 2213, 2834, 3298, 4312
BceAI	ACGGC	4 cut(s)	492, 2435, 4583, 4889
BcgI	CGANNNNNNTGC	4 cut(s)	1744, 1778, 4812, 4846
BciVI	GTATCC	4 cut(s)	49, 1702, 4278, 4549
BclI	TGATCA	2 cut(s)	1582, 2824
BcoDI	GTCTC	9 cut(s)	23, 1912, 1972, 2458, 3143, 3454, 3657, 4200, 4576
BcuI	ACTAGT	1 cut(s)	3845
BfmI	CTRYAG	6 cut(s)	608, 876, 1288, 1821, 3006, 3683
BfoI	RGCGCY	2 cut(s)	1329, 3205
BfrI	CTTAAG	2 cut(s)	1336, 1991
BfuI	GTATCC	4 cut(s)	49, 1702, 4278, 4549
BglI	GCCNNNNNGGC	1 cut(s)	3593
BglII	AGATCT	1 cut(s)	1547
BlnI	CCTAGG	1 cut(s)	4079
BmcAI	AGTACT	1 cut(s)	932
Bme18I	GGWCC	5 cut(s)	778, 1596, 2788, 4046, 4426
BmeT110I	CYCGRG	1 cut(s)	4710
BmgBI	CACGTC	3 cut(s)	1346, 2342, 4026
BmrI	ACTGGG	1 cut(s)	2610
BmuI	ACTGGG	1 cut(s)	2610
BpiI	GAAGAC	5 cut(s)	81, 2030, 2336, 3126, 4020
BpmI	CTGGAG	5 cut(s)	1406, 1919, 2724, 4388, 4578
Bpu10I	CCTNAGC	3 cut(s)	1037, 1778, 2754
Bpu14I	TTCGAA	1 cut(s)	1754
BpuEI	CTTGAG	3 cut(s)	2337, 3167, 3364
BsaAI	YACGTR	4 cut(s)	820, 2906, 3219, 3254
BsaBI	GATNNNNATC	2 cut(s)	906, 4206
BsaHI	GRCGYC	1 cut(s)	1326
BsaJI	CCNNGG	8 cut(s)	1631, 2158, 2494, 2510, 2539, 3114, 3430, 4079
BsaWI	WCCGGW	5 cut(s)	131, 1687, 2624, 3655, 4813
BsaXI	ACNNNNNCTCC	6 cut(s)	2697, 2727, 4236, 4266, 4664, 4694
Bse118I	RCCGGY	3 cut(s)	1687, 4796, 4813
Bse21I	CCTNAGG	1 cut(s)	643
Bse3DI	GCAATG	2 cut(s)	2892, 3782
Bse8I	GATNNNNATC	2 cut(s)	906, 4206
BseAI	TCCGGA	1 cut(s)	3655
BseDI	CCNNGG	8 cut(s)	1631, 2158, 2494, 2510, 2539, 3114, 3430, 4079
BseGI	GGATG	5 cut(s)	694, 1612, 1614, 3110, 4795
BseJI	GATNNNNATC	2 cut(s)	906, 4206
BseMI	GCAATG	2 cut(s)	2892, 3782
BseRI	GAGGAG	5 cut(s)	1308, 1673, 3574, 3783, 3822
BseSI	GKGCMC	2 cut(s)	3358, 3571
BseYI	CCCAGC	4 cut(s)	1536, 1695, 2897, 3344
BsgI	GTGCAG	3 cut(s)	876, 2718, 3594
Bsh1236I	CGCG	2 cut(s)	831, 1270
BshNI	GGYRCC	3 cut(s)	1325, 2653, 3872
BshTI	ACCGGT	2 cut(s)	1687, 4813
BsiHKCI	CYCGRG	1 cut(s)	4710
BsiSI	CCGG	8 cut(s)	132, 1688, 2625, 3656, 4479, 4670, 4797, 4814
BsiWI	CGTACG	1 cut(s)	4872
BslFI	GGGAC	3 cut(s)	764, 1605, 4000
BsmAI	GTCTC	9 cut(s)	23, 1912, 1972, 2458, 3143, 3454, 3657, 4200, 4576
BsmBI	CGTCTC	2 cut(s)	1972, 2458
BsmFI	GGGAC	3 cut(s)	764, 1605, 4000
BsoBI	CYCGRG	1 cut(s)	4710
Bsp119I	TTCGAA	1 cut(s)	1754
Bsp120I	GGGCCC	1 cut(s)	3354
Bsp1286I	GDGCHC	3 cut(s)	2468, 3358, 3571
Bsp13I	TCCGGA	1 cut(s)	3655
Bsp19I	CCATGG	2 cut(s)	2158, 3430
BspEI	TCCGGA	1 cut(s)	3655
BspFNI	CGCG	2 cut(s)	831, 1270
BspMAI	CTGCAG	5 cut(s)	612, 880, 1292, 1825, 3687
BspPI	GGATC	9 cut(s)	116, 220, 380, 902, 2067, 3011, 3814, 4337, 4862
BspT104I	TTCGAA	1 cut(s)	1754
BspT107I	GGYRCC	3 cut(s)	1325, 2653, 3872
BspTI	CTTAAG	2 cut(s)	1336, 1991
BsrDI	GCAATG	2 cut(s)	2892, 3782
BsrFI	RCCGGY	3 cut(s)	1687, 4796, 4813
BssAI	RCCGGY	3 cut(s)	1687, 4796, 4813
BssECI	CCNNGG	8 cut(s)	1631, 2158, 2494, 2510, 2539, 3114, 3430, 4079
BssNI	GRCGYC	1 cut(s)	1326
BssSI	CACGAG	2 cut(s)	293, 2421
BssT1I	CCWWGG	6 cut(s)	2158, 2494, 2510, 3114, 3430, 4079
Bst2BI	CACGAG	2 cut(s)	293, 2421
Bst6I	CTCTTC	4 cut(s)	609, 1538, 1631, 2511
BstACI	GRCGYC	1 cut(s)	1326
BstAFI	CTTAAG	2 cut(s)	1336, 1991
BstAPI	GCANNNNNTGC	1 cut(s)	3581
BstBAI	YACGTR	4 cut(s)	820, 2906, 3219, 3254
BstBI	TTCGAA	1 cut(s)	1754
BstDSI	CCRYGG	3 cut(s)	1631, 2158, 3430
BstEII	GGTNACC	1 cut(s)	4519
BstENI	CCTNNNNNAGG	2 cut(s)	336, 915
BstF5I	GGATG	5 cut(s)	694, 1612, 1614, 3110, 4795
BstFNI	CGCG	2 cut(s)	831, 1270
BstH2I	RGCGCY	2 cut(s)	1329, 3205
BstHHI	GCGC	2 cut(s)	1328, 3204
BstMAI	GTCTC	9 cut(s)	23, 1912, 1972, 2458, 3143, 3454, 3657, 4200, 4576
BstNSI	RCATGY	5 cut(s)	119, 251, 1163, 2011, 4088
BstPI	GGTNACC	1 cut(s)	4519
BstSFI	CTRYAG	6 cut(s)	608, 876, 1288, 1821, 3006, 3683
BstSLI	GKGCMC	2 cut(s)	3358, 3571
BstUI	CGCG	2 cut(s)	831, 1270
BstV2I	GAAGAC	5 cut(s)	81, 2030, 2336, 3126, 4020
BstX2I	RGATCY	2 cut(s)	1547, 2059
BstXI	CCANNNNNNTGG	4 cut(s)	849, 1536, 2686, 3611
BstYI	RGATCY	2 cut(s)	1547, 2059
Bsu36I	CCTNAGG	1 cut(s)	643
BsuI	GTATCC	4 cut(s)	49, 1702, 4278, 4549
BtgI	CCRYGG	3 cut(s)	1631, 2158, 3430
BtgZI	GCGATG	2 cut(s)	3516, 4706
BtrI	CACGTC	3 cut(s)	1346, 2342, 4026
BtsCI	GGATG	5 cut(s)	694, 1612, 1614, 3110, 4795
BtsI	GCAGTG	3 cut(s)	1445, 3095, 3706
BtsIMutI	CAGTG	9 cut(s)	217, 1227, 1445, 2598, 2664, 2745, 3095, 3706, 4382
CaiI	CAGNNNCTG	2 cut(s)	2333, 3581
CfoI	GCGC	2 cut(s)	1328, 3204
Cfr10I	RCCGGY	3 cut(s)	1687, 4796, 4813
CseI	GACGC	2 cut(s)	837, 2217
CsiI	ACCWGGT	1 cut(s)	4153
CspAI	ACCGGT	2 cut(s)	1687, 4813
CspCI	CAANNNNNGTGG	2 cut(s)	3832, 3867
DinI	GGCGCC	1 cut(s)	1327
DraI	TTTAAA	2 cut(s)	1095, 4741
DrdI	GACNNNNNNGTC	1 cut(s)	107
DseDI	GACNNNNNNGTC	1 cut(s)	107
EaeI	YGGCCR	2 cut(s)	297, 4836
Eam1104I	CTCTTC	4 cut(s)	609, 1538, 1631, 2511
EarI	CTCTTC	4 cut(s)	609, 1538, 1631, 2511
Eco130I	CCWWGG	6 cut(s)	2158, 2494, 2510, 3114, 3430, 4079
Eco147I	AGGCCT	2 cut(s)	3596, 4513
Eco24I	GRGCYC	2 cut(s)	2468, 3358
Eco32I	GATATC	1 cut(s)	487
Eco47I	GGWCC	5 cut(s)	778, 1596, 2788, 4046, 4426
Eco47III	AGCGCT	1 cut(s)	3203
Eco57I	CTGAAG	2 cut(s)	371, 2558
Eco72I	CACGTG	2 cut(s)	3219, 3254
Eco81I	CCTNAGG	1 cut(s)	643
Eco88I	CYCGRG	1 cut(s)	4710
Eco91I	GGTNACC	1 cut(s)	4519
EcoNI	CCTNNNNNAGG	2 cut(s)	336, 915
EcoO109I	RGGNCCY	4 cut(s)	778, 2537, 3354, 3355
EcoO65I	GGTNACC	1 cut(s)	4519
EcoRI	GAATTC	2 cut(s)	727, 1134
EcoRV	GATATC	1 cut(s)	487
EcoT14I	CCWWGG	6 cut(s)	2158, 2494, 2510, 3114, 3430, 4079
EcoT22I	ATGCAT	4 cut(s)	1614, 1767, 3523, 3995
EcoT38I	GRGCYC	2 cut(s)	2468, 3358
EgeI	GGCGCC	1 cut(s)	1327
EheI	GGCGCC	1 cut(s)	1327
ErhI	CCWWGG	6 cut(s)	2158, 2494, 2510, 3114, 3430, 4079
Esp3I	CGTCTC	2 cut(s)	1972, 2458
FalI	AAGNNNNNCTT	4 cut(s)	452, 484, 2391, 2423
FaqI	GGGAC	3 cut(s)	764, 1605, 4000
FauI	CCCGC	3 cut(s)	1263, 1714, 4733
FbaI	TGATCA	2 cut(s)	1582, 2824
FblI	GTMKAC	2 cut(s)	1875, 4575
FokI	GGATG	5 cut(s)	681, 1599, 1621, 3097, 4802
FriOI	GRGCYC	2 cut(s)	2468, 3358
GlaI	GCGC	2 cut(s)	1327, 3203
GsaI	CCCAGC	4 cut(s)	1540, 1699, 2901, 3348
GsuI	CTGGAG	5 cut(s)	1406, 1919, 2724, 4388, 4578
HaeII	RGCGCY	2 cut(s)	1329, 3205
HapII	CCGG	8 cut(s)	132, 1688, 2625, 3656, 4479, 4670, 4797, 4814
HgaI	GACGC	2 cut(s)	837, 2217
HhaI	GCGC	2 cut(s)	1328, 3204
Hin1I	GRCGYC	1 cut(s)	1326
Hin6I	GCGC	2 cut(s)	1326, 3202
HinP1I	GCGC	2 cut(s)	1326, 3202
HincII	GTYRAC	4 cut(s)	309, 1110, 1674, 2194
HindII	GTYRAC	4 cut(s)	309, 1110, 1674, 2194
HpaII	CCGG	8 cut(s)	132, 1688, 2625, 3656, 4479, 4670, 4797, 4814
Hpy99I	CGWCG	2 cut(s)	684, 831
Hsp92I	GRCGYC	1 cut(s)	1326
HspAI	GCGC	2 cut(s)	1326, 3202
KasI	GGCGCC	1 cut(s)	1325
KflI	GGGWCCC	1 cut(s)	778
Kpn2I	TCCGGA	1 cut(s)	3655
KpnI	GGTACC	1 cut(s)	2657
Ksp22I	TGATCA	2 cut(s)	1582, 2824
MabI	ACCWGGT	1 cut(s)	4153
MfeI	CAATTG	1 cut(s)	1017
MflI	RGATCY	2 cut(s)	1547, 2059
MhlI	GDGCHC	3 cut(s)	2468, 3358, 3571
MlsI	TGGCCA	2 cut(s)	299, 4838
MluNI	TGGCCA	2 cut(s)	299, 4838
Mly113I	GGCGCC	1 cut(s)	1326
MlyI	GAGTC	1 cut(s)	1000
MmeI	TCCRAC	3 cut(s)	974, 4276, 4404
Mox20I	TGGCCA	2 cut(s)	299, 4838
Mph1103I	ATGCAT	4 cut(s)	1614, 1767, 3523, 3995
MroI	TCCGGA	1 cut(s)	3655
MroXI	GAANNNNTTC	1 cut(s)	2958
MscI	TGGCCA	2 cut(s)	299, 4838
MslI	CAYNNNNRTG	2 cut(s)	818, 820
Msp20I	TGGCCA	2 cut(s)	299, 4838
MspA1I	CMGCKG	2 cut(s)	11, 1852
MspCI	CTTAAG	2 cut(s)	1336, 1991
MspI	CCGG	8 cut(s)	132, 1688, 2625, 3656, 4479, 4670, 4797, 4814
MssI	GTTTAAAC	1 cut(s)	4741
MunI	CAATTG	1 cut(s)	1017
MvnI	CGCG	2 cut(s)	831, 1270
NarI	GGCGCC	1 cut(s)	1326
NcoI	CCATGG	2 cut(s)	2158, 3430
NmeAIII	GCCGAG	1 cut(s)	4207
NmuCI	GTSAC	5 cut(s)	46, 355, 598, 2647, 2768
NsiI	ATGCAT	4 cut(s)	1614, 1767, 3523, 3995
NspI	RCATGY	5 cut(s)	119, 251, 1163, 2011, 4088
NspV	TTCGAA	1 cut(s)	1754
OliI	CACNNNNGTG	1 cut(s)	818
PaeI	GCATGC	1 cut(s)	1163
PaeR7I	CTCGAG	1 cut(s)	4710
PceI	AGGCCT	2 cut(s)	3596, 4513
PciI	ACATGT	2 cut(s)	115, 2007
PcsI	WCGNNNNNNNCGW	1 cut(s)	4147
PdmI	GAANNNNTTC	1 cut(s)	2958
Pfl23II	CGTACG	1 cut(s)	4872
PflFI	GACNNNGTC	1 cut(s)	3536
PflMI	CCANNNNNTGG	1 cut(s)	1605
PfoI	TCCNGGA	1 cut(s)	4626
PinAI	ACCGGT	2 cut(s)	1687, 4813
PleI	GAGTC	1 cut(s)	999
PluTI	GGCGCC	1 cut(s)	1329
PmaCI	CACGTG	2 cut(s)	3219, 3254
PmeI	GTTTAAAC	1 cut(s)	4741
PmlI	CACGTG	2 cut(s)	3219, 3254
PpsI	GAGTC	1 cut(s)	999
Ppu21I	YACGTR	4 cut(s)	820, 2906, 3219, 3254
PpuMI	RGGWCCY	1 cut(s)	778
PscI	ACATGT	2 cut(s)	115, 2007
PshBI	ATTAAT	1 cut(s)	453
PsiI	TTATAA	1 cut(s)	915
Psp5II	RGGWCCY	1 cut(s)	778
PspCI	CACGTG	2 cut(s)	3219, 3254
PspEI	GGTNACC	1 cut(s)	4519
PspFI	CCCAGC	4 cut(s)	1536, 1695, 2897, 3344
PspLI	CGTACG	1 cut(s)	4872
PspOMI	GGGCCC	1 cut(s)	3354
PspPPI	RGGWCCY	1 cut(s)	778
PstI	CTGCAG	5 cut(s)	612, 880, 1292, 1825, 3687
PstNI	CAGNNNCTG	2 cut(s)	2333, 3581
PsuI	RGATCY	2 cut(s)	1547, 2059
PsyI	GACNNNGTC	1 cut(s)	3536
PvuII	CAGCTG	1 cut(s)	11
RseI	CAYNNNNRTG	2 cut(s)	818, 820
ScaI	AGTACT	1 cut(s)	932
SchI	GAGTC	1 cut(s)	1000
SduI	GDGCHC	3 cut(s)	2468, 3358, 3571
SexAI	ACCWGGT	1 cut(s)	4153
SfcI	CTRYAG	6 cut(s)	608, 876, 1288, 1821, 3006, 3683
SfoI	GGCGCC	1 cut(s)	1327
Sfr274I	CTCGAG	1 cut(s)	4710
SfuI	TTCGAA	1 cut(s)	1754
SinI	GGWCC	5 cut(s)	778, 1596, 2788, 4046, 4426
SlaI	CTCGAG	1 cut(s)	4710
SmiMI	CAYNNNNRTG	2 cut(s)	818, 820
SmlI	CTYRAG	6 cut(s)	1336, 1991, 2352, 3146, 3379, 4710
SmoI	CTYRAG	6 cut(s)	1336, 1991, 2352, 3146, 3379, 4710
SpeI	ACTAGT	1 cut(s)	3845
SphI	GCATGC	1 cut(s)	1163
SseBI	AGGCCT	2 cut(s)	3596, 4513
SspDI	GGCGCC	1 cut(s)	1325
SspI	AATATT	2 cut(s)	255, 4040
StuI	AGGCCT	2 cut(s)	3596, 4513
StyI	CCWWGG	6 cut(s)	2158, 2494, 2510, 3114, 3430, 4079
TaqII	GACCGA	1 cut(s)	3679
TatI	WGTACW	4 cut(s)	233, 930, 2871, 4695
TauI	GCSGC	6 cut(s)	834, 1450, 2087, 3216, 4122, 4823
TscAI	CASTG	9 cut(s)	224, 1234, 1445, 2605, 2664, 2745, 3102, 3706, 4389
TseFI	GTSAC	5 cut(s)	46, 355, 598, 2647, 2768
Tsp45I	GTSAC	5 cut(s)	46, 355, 598, 2647, 2768
TspGWI	ACGGA	6 cut(s)	225, 672, 1605, 3079, 4796, 4860
TspRI	CASTG	9 cut(s)	224, 1234, 1445, 2605, 2664, 2745, 3102, 3706, 4389
Tth111I	GACNNNGTC	1 cut(s)	3536
Van91I	CCANNNNNTGG	1 cut(s)	1605
Vha464I	CTTAAG	2 cut(s)	1336, 1991
VpaK11BI	GGWCC	5 cut(s)	778, 1596, 2788, 4046, 4426
VspI	ATTAAT	1 cut(s)	453
XagI	CCTNNNNNAGG	2 cut(s)	336, 915
XapI	RAATTY	7 cut(s)	325, 536, 727, 967, 1134, 1805, 4097
XceI	RCATGY	5 cut(s)	119, 251, 1163, 2011, 4088
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4285
XhoI	CTCGAG	1 cut(s)	4710
XmaJI	CCTAGG	1 cut(s)	4079
XmiI	GTMKAC	2 cut(s)	1875, 4575
XmnI	GAANNNNTTC	1 cut(s)	2958
ZrmI	AGTACT	1 cut(s)	932
Zsp2I	ATGCAT	4 cut(s)	1614, 1767, 3523, 3995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.