MD02G1009100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009367
Physical Location & Seq
Forward (+)
602981 .. 604595
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1009100.v1.1.491

Sequence Viewer

Length: 306 bp
ATGGCCAAGGAAGCTTACGCCGCACGAAACCCAGATGAGGTTCACACAGACGTGCTCTCCAATGCCAGACAAGCTTGCTATAAGGCACGGGACGCTTTCTATGCGTGTGTGGAGAAAGAATCGGACAAGAAGCAGACCGAAATCGCATCGAAAGGCCAGTTGTACGCAGCGGAATGCAAAGCTTCGAGGGAGGAGTTTGTCAAACACTGCCGTACTTCTTGGGTGAAGCATTTTGATGCGCTCGACTGCAAGAACAAGAGTCTGAAGAGGCTTTTGGATGATAAGGATTCGAGGAGAGGGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
COX6B

Protein Analysis

102

Amino Acids

11.57

Weight (kDa)

8.81

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX6B PF02297 20 - 87 2e-14 Cytochrome oxidase c subunit VIb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 21, 170
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 284
AfaI GTAC 2 cut(s) 164, 214
AfiI CCNNNNNNNGG 1 cut(s) 37
AjiI CACGTC 1 cut(s) 52
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 3 cut(s) 14, 74, 182
AluI AGCT 3 cut(s) 14, 74, 182
Alw21I GWGCWC 1 cut(s) 57
AoxI GGCC 2 cut(s) 3, 154
ApeKI GCWGC 1 cut(s) 167
AspLEI GCGC 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 235
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 195
BisI GCNGC 2 cut(s) 21, 168
BlsI GCNGC 2 cut(s) 22, 169
BmgBI CACGTC 1 cut(s) 52
BmsI GCATC 2 cut(s) 155, 226
BsaJI CCNNGG 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 157
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 1 cut(s) 37
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 179
BshFI GGCC 2 cut(s) 5, 156
BsiHKAI GWGCWC 1 cut(s) 57
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 2 cut(s) 5, 156
Bsp1286I GDGCHC 1 cut(s) 57
BspACI CCGC 2 cut(s) 21, 170
BspANI GGCC 2 cut(s) 5, 156
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 6
Bst6I CTCTTC 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 76
BstF5I GGATG 1 cut(s) 283
BstHHI GCGC 1 cut(s) 241
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 71, 92, 101
BstV1I GCAGC 1 cut(s) 179
BsuRI GGCC 2 cut(s) 5, 156
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 1 cut(s) 283
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 241
CseI GACGC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 163, 213
CviJI RGCY 6 cut(s) 5, 14, 74, 156, 182, 271
CviKI_1 RGCY 6 cut(s) 5, 14, 74, 156, 182, 271
CviQI GTAC 2 cut(s) 163, 213
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaiI YATR 3 cut(s) 81, 102, 304
FaqI GGGAC 1 cut(s) 104
Fnu4HI GCNGC 2 cut(s) 21, 168
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 2 cut(s) 21, 168
GlaI GCGC 1 cut(s) 240
GluI GCNGC 2 cut(s) 21, 168
HaeIII GGCC 2 cut(s) 5, 156
HgaI GACGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 241
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HindIII AAGCTT 3 cut(s) 12, 72, 180
HinfI GANTC 3 cut(s) 119, 259, 287
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 124, 264
Hpy8I GTNNAC 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 177, 249
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 71, 92, 101
HpySE526I ACGT 1 cut(s) 51
HspAI GCGC 1 cut(s) 239
LpnPI CCDG 3 cut(s) 45, 79, 170
Lsp1109I GCAGC 1 cut(s) 179
LweI GCATC 2 cut(s) 155, 226
MaeII ACGT 1 cut(s) 51
MboII GAAGA 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 57
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 268
MnlI CCTC 6 cut(s) 31, 180, 184, 261, 285, 290
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 50, 234
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 179
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 71, 92, 101
OliI CACNNNNGTG 1 cut(s) 50
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 119, 287
PkrI GCNGC 2 cut(s) 22, 169
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
RsaI GTAC 2 cut(s) 164, 214
RsaNI GTAC 2 cut(s) 163, 213
RseI CAYNNNNRTG 2 cut(s) 50, 234
SatI GCNGC 2 cut(s) 21, 168
SchI GAGTC 1 cut(s) 268
SduI GDGCHC 1 cut(s) 57
SetI ASST 5 cut(s) 16, 42, 54, 76, 184
SfaNI GCATC 2 cut(s) 155, 226
SmiMI CAYNNNNRTG 2 cut(s) 50, 234
SsiI CCGC 2 cut(s) 21, 170
StyI CCWWGG 1 cut(s) 6
TaiI ACGT 1 cut(s) 54
TaqI TCGA 4 cut(s) 149, 185, 243, 290
TaqII GACCGA 1 cut(s) 152
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 2 cut(s) 119, 287
TscAI CASTG 1 cut(s) 212
TseI GCWGC 1 cut(s) 167
TspRI CASTG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.