Rh2CG014300

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009367
Physical Location & Seq
Forward (+)
1175128 .. 1176111
984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG014300.1

Sequence Viewer

Length: 477 bp
ATGGTGATTAGGCCCACTGGGCCAACACGGCCCATTCACTTCACCCAAACTCCCTTTCATTGGTGCACCTACAGTCGCGCTAATCGTACTCTTGCAGAAGCAAAACGTTCTCTCTTTAGCGAAGCCATGGCCAATGAAGCTTTAACAGCACGAAACCCAGATGGGGTTCACACCGATGTGCTCTCTAGTGCAAGACAAGCATGCTATAAGGCACGAGACGCATTCTATGCTTGTTTGGAGAAAGAATCAGATAAGAAACAGACTGAAATTGCGTCCAAGGGTCTGTTATACCCAGCACAGTGCAAACCCGCCAGGGATCAGTTCGTCAATAACTGCCGTGCTTCTTGGGTGAAGCATTTTGATGCGATGCACTGCAAGAACAGAAGTTTGAAGAGGCTTTTGGATGATAAAGAATCGAGGAGAGGTCCGTTGTCACTTCCACAACCTTACACTTTCAAGCCCCCCAGCACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
COX6B

Protein Analysis

158

Amino Acids

17.98

Weight (kDa)

9.63

Isoelectric Point (pI)

57.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX6B PF02297 62 - 128 1.3e-14 Cytochrome oxidase c subunit VIb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 78
AciI CCGC 1 cut(s) 309
AclI AACGTT 1 cut(s) 106
AclWI GGATC 1 cut(s) 324
AcoI YGGCCR 1 cut(s) 129
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 2 cut(s) 60, 163
AgsI TTSAA 2 cut(s) 391, 457
AhlI ACTAGT 1 cut(s) 470
AjnI CCWGG 1 cut(s) 311
AjuI GAANNNNNNNTTGG 4 cut(s) 39, 71, 383, 415
AleI CACNNNNGTG 1 cut(s) 176
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
Alw21I GWGCWC 2 cut(s) 68, 183
Alw26I GTCTC 1 cut(s) 210
Alw44I GTGCAC 1 cut(s) 64
AlwI GGATC 1 cut(s) 324
AoxI GGCC 4 cut(s) 11, 20, 29, 129
ApaLI GTGCAC 1 cut(s) 64
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 4 cut(s) 12, 20, 30, 425
AsuHPI GGTGA 3 cut(s) 16, 34, 361
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 68
BalI TGGCCA 1 cut(s) 131
BauI CACGAG 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 68, 183
BccI CCATC 1 cut(s) 155
BceAI ACGGC 2 cut(s) 44, 321
BciT130I CCWGG 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 210
BcuI ACTAGT 1 cut(s) 470
BfaI CTAG 2 cut(s) 186, 471
BfmI CTRYAG 1 cut(s) 70
BglI GCCNNNNNGGC 2 cut(s) 19, 28
Bme1390I CCNGG 1 cut(s) 313
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 4 cut(s) 12, 20, 30, 425
BmrFI CCNGG 1 cut(s) 313
BmrI ACTGGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 352, 357
BmuI ACTGGG 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 126, 276, 312
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 163
Bse1I ACTGG 1 cut(s) 22
BseBI CCWGG 1 cut(s) 313
BseDI CCNNGG 3 cut(s) 126, 276, 312
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 2 cut(s) 60, 163
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 1 cut(s) 433
BseSI GKGCMC 1 cut(s) 68
BseYI CCCAGC 2 cut(s) 292, 464
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 4 cut(s) 13, 22, 31, 131
BsiHKAI GWGCWC 2 cut(s) 68, 183
BslI CCNNNNNNNGG 2 cut(s) 60, 163
BsmAI GTCTC 1 cut(s) 210
BsmBI CGTCTC 1 cut(s) 210
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 4 cut(s) 13, 22, 31, 131
Bsp1286I GDGCHC 2 cut(s) 68, 183
Bsp143I GATC 1 cut(s) 316
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 1 cut(s) 309
BspANI GGCC 4 cut(s) 13, 22, 31, 131
BspFNI CGCG 1 cut(s) 78
BspPI GGATC 1 cut(s) 324
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 3 cut(s) 126, 276, 312
BssMI GATC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 126, 276
Bst2BI CACGAG 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 313
Bst4CI ACNGT 2 cut(s) 74, 300
Bst6I CTCTTC 1 cut(s) 386
BstAPI GCANNNNNTGC 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 202
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 409
BstFNI CGCG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 7 cut(s) 19, 28, 137, 146, 197, 218, 227
BstNI CCWGG 1 cut(s) 313
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 311
BstSFI CTRYAG 1 cut(s) 70
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 1 cut(s) 78
BsuRI GGCC 4 cut(s) 13, 22, 31, 131
BtgI CCRYGG 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 380
BtsCI GGATG 1 cut(s) 409
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 3 cut(s) 15, 305, 370
Cac8I GCNNGC 1 cut(s) 202
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 4 cut(s) 12, 20, 30, 425
CseI GACGC 2 cut(s) 227, 261
Csp6I GTAC 1 cut(s) 87
CviAII CATG 2 cut(s) 127, 201
CviJI RGCY 8 cut(s) 13, 22, 31, 125, 131, 140, 397, 460
CviKI_1 RGCY 8 cut(s) 13, 22, 31, 125, 131, 140, 397, 460
CviQI GTAC 1 cut(s) 87
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Eco130I CCWWGG 2 cut(s) 126, 276
Eco47I GGWCC 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 311
EcoT14I CCWWGG 2 cut(s) 126, 276
ErhI CCWWGG 2 cut(s) 126, 276
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 2 cut(s) 130, 204
FaiI YATR 5 cut(s) 128, 202, 207, 228, 289
FatI CATG 2 cut(s) 126, 200
FauI CCCGC 1 cut(s) 316
FokI GGATG 1 cut(s) 416
FspBI CTAG 2 cut(s) 186, 471
GlaI GCGC 1 cut(s) 79
GsaI CCCAGC 2 cut(s) 296, 468
HaeIII GGCC 4 cut(s) 13, 22, 31, 131
HgaI GACGC 2 cut(s) 227, 261
HhaI GCGC 1 cut(s) 80
Hin1II CATG 2 cut(s) 130, 204
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 2 cut(s) 245, 413
HphI GGTGA 3 cut(s) 16, 34, 361
Hpy166II GTNNAC 2 cut(s) 66, 169
Hpy188I TCNGA 1 cut(s) 250
Hpy8I GTNNAC 2 cut(s) 66, 169
HpyCH4III ACNGT 2 cut(s) 74, 300
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 6 cut(s) 66, 95, 191, 303, 370, 375
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 28, 137, 146, 197, 218, 227
HpySE526I ACGT 1 cut(s) 106
Hsp92II CATG 2 cut(s) 130, 204
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 1 cut(s) 316
LpnPI CCDG 5 cut(s) 3, 171, 298, 306, 325
LweI GCATC 2 cut(s) 352, 357
MaeI CTAG 2 cut(s) 186, 471
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 1 cut(s) 432
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 1 cut(s) 403
MhlI GDGCHC 2 cut(s) 68, 183
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 131
MnlI CCTC 3 cut(s) 387, 411, 416
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 2 cut(s) 143, 475
MslI CAYNNNNRTG 3 cut(s) 174, 176, 360
Msp20I TGGCCA 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 313
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 1 cut(s) 313
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 7 cut(s) 19, 28, 137, 146, 197, 218, 227
NcoI CCATGG 1 cut(s) 126
NdeII GATC 1 cut(s) 316
NlaIII CATG 2 cut(s) 130, 204
NmuCI GTSAC 1 cut(s) 432
NspI RCATGY 1 cut(s) 204
OliI CACNNNNGTG 1 cut(s) 176
PaeI GCATGC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 245, 413
Psp1406I AACGTT 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 311
PspFI CCCAGC 2 cut(s) 292, 464
PspGI CCWGG 1 cut(s) 311
PspPI GGNCC 4 cut(s) 12, 20, 30, 425
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
RseI CAYNNNNRTG 3 cut(s) 174, 176, 360
SaqAI TTAA 2 cut(s) 143, 475
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 4 cut(s) 12, 20, 30, 425
ScrFI CCNGG 1 cut(s) 313
SduI GDGCHC 2 cut(s) 68, 183
SetI ASST 5 cut(s) 71, 109, 142, 427, 448
SfaNI GCATC 2 cut(s) 352, 357
SfcI CTRYAG 1 cut(s) 70
SfiI GGCCNNNNNGGCC 2 cut(s) 19, 28
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 3 cut(s) 174, 176, 360
SpeI ACTAGT 1 cut(s) 470
SphI GCATGC 1 cut(s) 204
Sse9I AATT 1 cut(s) 267
SsiI CCGC 1 cut(s) 309
SspMI CTAG 2 cut(s) 186, 471
StyD4I CCNGG 1 cut(s) 311
StyI CCWWGG 2 cut(s) 126, 276
TaaI ACNGT 2 cut(s) 74, 300
TaiI ACGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 416
TasI AATT 1 cut(s) 267
TfiI GAWTC 2 cut(s) 245, 413
Tru1I TTAA 2 cut(s) 143, 475
Tru9I TTAA 2 cut(s) 143, 475
TscAI CASTG 3 cut(s) 22, 305, 377
TseFI GTSAC 1 cut(s) 432
Tsp45I GTSAC 1 cut(s) 432
TspDTI ATGAA 2 cut(s) 47, 150
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 3 cut(s) 22, 305, 377
VneI GTGCAC 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 425
XceI RCATGY 1 cut(s) 204
XspI CTAG 2 cut(s) 186, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.