Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010512

Physical Location & Seq

Genomic Coordinates Reverse (-)

850298 .. 854762

Gene Structure 4465 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1013400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGAAAATTGAATTTGAAATAAAAAAGGAAAAATGGAAAGCTCTCTCTCTCTCAGCCGGTTTGCTTCAATTATCATCATTCATCACCATCTCTGAAGCGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTTCCTCTCTCTCTCTCTCAAATGGGTTCGAGCAGCAGTCGACTGGGCTCGCGCCCATCTCGGCCCCGAGTCAATCGCACCGCCAGTCGTTCCATACTCTCCTCTTTCGTCTGTGGTGGCTCCTCTGCACGTGCTACTCACGAGATGGAAGATCACGCACCTGAGTGTCGTGTAAAATATGCAGAGCAATCTGATCTTGATATCAATCAAGTCCGGAACTCTGGAGAAGGATCCTCGTTGGTCTCCAGTAGTGGACCACTGTTCACCTGCCCCATTATTGAAATTGGATCATCATCTGAGAGCACTGTTGAAGAAGGATTAAGCAGTGTTGACACAATTAACCGGGGGAAGTGCTTGTCCAAAAGTAAAGAACTAATACCTCATCAGGTAAGTGCCGATTATAGTCATGATCAATCTTGTAGGGATAATAGTACTACCGCTAGTACTTCGTTTAGACAAGAACAATCTTCAGAAACAGTCTCTTTGAATGGTTCTGCTAATGTGGATTCAGTCAATGGCATTGACATTTCAGCAGACAAGAATGTATCTGAGACCTGTCCCGAGGTTATGCTTCCAAGTAGCTCATCTCCACAAGGGATTCGGAATTCCCGTTCTCAGGAAGTGTCTGTTGAGAATCATGTGGCTGACGTAAGGGGTGTACAGGGTTTTAATGCTGATACTGATTCTGCTAATGTTTCTGAAACTCCGTCGACTTTCGACTCACTTGGAAATGATTCCCTTCGGGAGGCAATACCTTCTGGTTTAGGATTCCTTGTGTCCAATAGGGAACAAGGCCAGGAAGATGGAAGTGTACTTCATGTTGATGTTATGAGCATTTCTTCCAACTTTTTGAGGAGTAGCAATGCTGATGCAAGTAATCGTGAGGCTAGAAGGAATGGCAGGCGACTGTTCTGGGATGCATTTTCAAGGCGCAGCTCCAGGAGGTTTATTGATTCACCAACCATTCTTTTCTCTACTGATGATACCGAGGATATGGGATTTCATGACAGATGGCTGCTTGGTTTGAGTGGTGATTTCTTTGATGATGAAATTGGAGTTGATTCTGGTCACCGGGCTAATAGAATTCCCAACTCGAGTGAACGACGGCGACATTCTAGATCTGAGGTCTGGGAAAGACTTCGTGTTGGCCTTGATGATAATGGCCAGCGTAATACCTCTTGTCCCACAGGAATCCACCCTCGTGGCATGTGCTCATGTGGCTCATTCTTGATGGCTGAAGAGTCTAGTACTCGGGCAAGTATATCAAGAATTGTCATGCTCGCCGAAGCTCTATTTGAGGTTTTGGATGAGATTCACCGCCAACCTGTGTCACTTTCACTGTCGATGGTCTCAGCTCCTGCCCCAGCATCTCTTGTTGATGGTTTTCCTCTTAAAAGTCACACAAAGGTTGACAAACCAAAGTCCGGGGATGATGTTGAACAGTGTTACATATGCCTCGCTGAGTATGAGGAAGGAGACAAAATTCGAGTTCTTCCCTGCCATCACGAGTATCACATGTCATGCGTTGATAAGTGGCTCAAAGAGATACACGGAGTTTGCCCGCTTTGCCGAGGAGATGTTCGTGAGGGTTTCACCGACTGTTCTGTCCCTAACTCGGAGATATCTTCTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000578 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0007389 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009798 GO:0009880 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048856 GO:0051603 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_11 zf-RING_2

Protein Analysis

589

Amino Acids

64.22

Weight (kDa)

5.28

Isoelectric Point (pI)

59.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	524 - 568	4.9e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	525 - 568	1.8e-12	Ring finger domain
zf-RING_11	PF17123	525 - 553	1.7e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	525 - 567	8.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	526 - 567	2.2e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010512)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g01770
malus_domestica	MD02G1013400.v1.1 MD15G1160200.v1.1
prunus_persica	Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1
pyrus_communis	pycom02g01250 pycom15g14410
rosa_chinensis	RchiOBHm_Chr2g0086731
rosa_laevigata	RLG00000015786
rosa_multiflora	Rmu_sc0000335.1_g000009 Rmu_sc0004626.1_g000001 Rmu_sc0030160.1_g000001 Rmu_sc0035211.1_g000001
rosa_roxburghii	Rroxscaffold_2G00154240
rosa_rugosa	Rorug01G0469200
rosa_samantha	Rh2AG021300 Rh2BG021200 Rh2CG022100 Rh2DG021900
rosa_wichuraiana	Rw2G001730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	407
AasI	GACNNNNNNGTC	1 cut(s)	1736
Acc36I	ACCTGC	1 cut(s)	407
AccI	GTMKAC	2 cut(s)	172, 842
AccII	CGCG	1 cut(s)	184
AccIII	TCCGGA	1 cut(s)	345
AciI	CCGC	4 cut(s)	213, 570, 1450, 1694
AclWI	GGATC	3 cut(s)	357, 370, 427
AcoI	YGGCCR	1 cut(s)	1294
AcsI	RAATTY	4 cut(s)	11, 736, 1215, 1614
AcuI	CTGAAG	3 cut(s)	114, 585, 1389
AcvI	CACGTG	1 cut(s)	263
AfaI	GTAC	5 cut(s)	565, 577, 792, 945, 1381
AfeI	AGCGCT	1 cut(s)	99
AfiI	CCNNNNNNNGG	4 cut(s)	748, 877, 1556, 1747
AflIII	ACRYGT	1 cut(s)	1647
AgsI	TTSAA	8 cut(s)	11, 17, 68, 413, 443, 619, 1059, 1571
AjnI	CCWGG	2 cut(s)	927, 1070
AleI	CACNNNNGTG	2 cut(s)	295, 1332
AloI	GAACNNNNNNTCC	2 cut(s)	727, 759
AluBI	AGCT	5 cut(s)	41, 714, 1068, 1421, 1487
AluI	AGCT	5 cut(s)	41, 714, 1068, 1421, 1487
Alw21I	GWGCWC	2 cut(s)	437, 1346
Alw26I	GTCTC	5 cut(s)	379, 616, 677, 1486, 1602
AlwI	GGATC	3 cut(s)	357, 370, 427
AlwNI	CAGNNNCTG	1 cut(s)	1490
Ama87I	CYCGRG	4 cut(s)	198, 692, 1225, 1383
Aor13HI	TCCGGA	1 cut(s)	345
Aor51HI	AGCGCT	1 cut(s)	99
AoxI	GGCC	4 cut(s)	194, 925, 1279, 1294
ApeKI	GCWGC	3 cut(s)	165, 1065, 1147
ApoI	RAATTY	4 cut(s)	11, 736, 1215, 1614
Asp700I	GAANNNNTTC	2 cut(s)	865, 1269
AspLEI	GCGC	3 cut(s)	100, 186, 1065
AspS9I	GGNCC	2 cut(s)	195, 386
AsuC2I	CCSGG	3 cut(s)	476, 1205, 1558
AsuHPI	GGTGA	7 cut(s)	76, 388, 1080, 1175, 1193, 1439, 1717
AvaI	CYCGRG	4 cut(s)	198, 692, 1225, 1383
AvaII	GGWCC	1 cut(s)	386
BalI	TGGCCA	1 cut(s)	1296
BamHI	GGATCC	1 cut(s)	362
BanII	GRGCYC	1 cut(s)	182
BauI	CACGAG	3 cut(s)	272, 1332, 1637
BbrPI	CACGTG	1 cut(s)	263
Bbv12I	GWGCWC	2 cut(s)	437, 1346
BbvI	GCAGC	3 cut(s)	177, 1077, 1134
BccI	CCATC	9 cut(s)	95, 196, 271, 929, 1137, 1357, 1471, 1505, 1641
BceAI	ACGGC	1 cut(s)	1253
BciT130I	CCWGG	2 cut(s)	929, 1072
BclI	TGATCA	1 cut(s)	541
BcnI	CCSGG	3 cut(s)	476, 1205, 1558
BcoDI	GTCTC	5 cut(s)	379, 616, 677, 1486, 1602
BfaI	CTAG	4 cut(s)	573, 1020, 1248, 1377
BfoI	RGCGCY	1 cut(s)	101
BfuAI	ACCTGC	1 cut(s)	407
BglII	AGATCT	1 cut(s)	1250
BisI	GCNGC	3 cut(s)	166, 1066, 1148
BlsI	GCNGC	3 cut(s)	167, 1067, 1149
BmcAI	AGTACT	3 cut(s)	565, 577, 1381
Bme1390I	CCNGG	5 cut(s)	476, 929, 1072, 1205, 1558
Bme18I	GGWCC	1 cut(s)	386
BmeT110I	CYCGRG	4 cut(s)	198, 692, 1225, 1383
BmgT120I	GGNCC	2 cut(s)	195, 386
BmiI	GGNNCC	3 cut(s)	197, 253, 364
BmrFI	CCNGG	5 cut(s)	476, 929, 1072, 1205, 1558
BmrI	ACTGGG	1 cut(s)	185
BmsI	GCATC	3 cut(s)	991, 1039, 1508
BmuI	ACTGGG	1 cut(s)	185
BpmI	CTGGAG	3 cut(s)	361, 375, 1054
BpuMI	CCSGG	3 cut(s)	476, 1205, 1558
BsaAI	YACGTR	1 cut(s)	263
BsaBI	GATNNNNATC	2 cut(s)	336, 424
BsaI	GGTCTC	3 cut(s)	379, 677, 1486
BsaJI	CCNNGG	5 cut(s)	475, 693, 1119, 1557, 1702
BsaWI	WCCGGW	1 cut(s)	345
Bsc4I	CCNNNNNNNGG	4 cut(s)	748, 877, 1556, 1747
Bse118I	RCCGGY	1 cut(s)	56
Bse1I	ACTGG	3 cut(s)	180, 216, 378
Bse3DI	GCAATG	1 cut(s)	1000
Bse8I	GATNNNNATC	2 cut(s)	336, 424
BseAI	TCCGGA	1 cut(s)	345
BseBI	CCWGG	2 cut(s)	929, 1072
BseDI	CCNNGG	5 cut(s)	475, 693, 1119, 1557, 1702
BseGI	GGATG	3 cut(s)	1054, 1444, 1567
BseJI	GATNNNNATC	2 cut(s)	336, 424
BseLI	CCNNNNNNNGG	4 cut(s)	748, 877, 1556, 1747
BseMI	GCAATG	1 cut(s)	1000
BseMII	CTCAG	8 cut(s)	66, 285, 420, 672, 761, 1245, 1497, 1584
BseNI	ACTGG	3 cut(s)	180, 216, 378
BseRI	GAGGAG	4 cut(s)	223, 244, 1000, 1719
BseXI	GCAGC	3 cut(s)	177, 1077, 1134
BseYI	CCCAGC	1 cut(s)	1495
BsgI	GTGCAG	1 cut(s)	243
Bsh1236I	CGCG	1 cut(s)	184
BshFI	GGCC	4 cut(s)	196, 927, 1281, 1296
BsiHKAI	GWGCWC	2 cut(s)	437, 1346
BsiHKCI	CYCGRG	4 cut(s)	198, 692, 1225, 1383
BsiSI	CCGG	5 cut(s)	57, 346, 475, 1204, 1557
BslFI	GGGAC	3 cut(s)	675, 1299, 1724
BslI	CCNNNNNNNGG	4 cut(s)	748, 877, 1556, 1747
BsmAI	GTCTC	5 cut(s)	379, 616, 677, 1486, 1602
BsmFI	GGGAC	3 cut(s)	675, 1299, 1724
BsnI	GGCC	4 cut(s)	196, 927, 1281, 1296
Bso31I	GGTCTC	3 cut(s)	379, 677, 1486
BsoBI	CYCGRG	4 cut(s)	198, 692, 1225, 1383
Bsp1286I	GDGCHC	3 cut(s)	182, 437, 1346
Bsp13I	TCCGGA	1 cut(s)	345
Bsp1407I	TGTACA	1 cut(s)	790
Bsp143I	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
BspACI	CCGC	4 cut(s)	213, 570, 1450, 1694
BspANI	GGCC	4 cut(s)	196, 927, 1281, 1296
BspCNI	CTCAG	8 cut(s)	65, 286, 421, 673, 760, 1246, 1496, 1585
BspEI	TCCGGA	1 cut(s)	345
BspFNI	CGCG	1 cut(s)	184
BspHI	TCATGA	2 cut(s)	538, 1135
BspLI	GGNNCC	3 cut(s)	197, 253, 364
BspMI	ACCTGC	1 cut(s)	407
BspPI	GGATC	3 cut(s)	357, 370, 427
BspTNI	GGTCTC	3 cut(s)	379, 677, 1486
BsrDI	GCAATG	1 cut(s)	1000
BsrFI	RCCGGY	1 cut(s)	56
BsrGI	TGTACA	1 cut(s)	790
BsrI	ACTGG	3 cut(s)	180, 216, 378
BssAI	RCCGGY	1 cut(s)	56
BssECI	CCNNGG	5 cut(s)	475, 693, 1119, 1557, 1702
BssMI	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
BssSI	CACGAG	3 cut(s)	272, 1332, 1637
Bst2BI	CACGAG	3 cut(s)	272, 1332, 1637
Bst2UI	CCWGG	2 cut(s)	929, 1072
Bst4CI	ACNGT	7 cut(s)	393, 439, 610, 1041, 1473, 1575, 1733
Bst6I	CTCTTC	1 cut(s)	1365
BstAUI	TGTACA	1 cut(s)	790
BstBAI	YACGTR	1 cut(s)	263
BstC8I	GCNNGC	5 cut(s)	182, 1034, 1298, 1413, 1694
BstDEI	CTNAG	8 cut(s)	52, 294, 429, 681, 747, 1254, 1483, 1593
BstEII	GGTNACC	1 cut(s)	1199
BstENI	CCTNNNNNAGG	1 cut(s)	875
BstF5I	GGATG	3 cut(s)	1054, 1444, 1567
BstFNI	CGCG	1 cut(s)	184
BstH2I	RGCGCY	1 cut(s)	101
BstHHI	GCGC	3 cut(s)	100, 186, 1065
BstKTI	GATC	6 cut(s)	286, 328, 365, 422, 544, 1253
BstMAI	GTCTC	5 cut(s)	379, 616, 677, 1486, 1602
BstMBI	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
BstMWI	GCNNNNNNNGC	2 cut(s)	1350, 1698
BstNI	CCWGG	2 cut(s)	929, 1072
BstNSI	RCATGY	2 cut(s)	1342, 1651
BstPI	GGTNACC	1 cut(s)	1199
BstSCI	CCNGG	5 cut(s)	474, 927, 1070, 1203, 1556
BstUI	CGCG	1 cut(s)	184
BstV1I	GCAGC	3 cut(s)	177, 1077, 1134
BstX2I	RGATCY	2 cut(s)	362, 1250
BstXI	CCANNNNNNTGG	2 cut(s)	935, 1334
BstYI	RGATCY	2 cut(s)	362, 1250
BsuRI	GGCC	4 cut(s)	196, 927, 1281, 1296
BtsCI	GGATG	3 cut(s)	1054, 1444, 1567
BtsI	GCAGTG	1 cut(s)	463
BtsIMutI	CAGTG	5 cut(s)	389, 435, 463, 1469, 1580
BveI	ACCTGC	1 cut(s)	407
Cac8I	GCNNGC	5 cut(s)	182, 1034, 1298, 1413, 1694
CaiI	CAGNNNCTG	1 cut(s)	1490
CciI	TCATGA	2 cut(s)	538, 1135
CfoI	GCGC	3 cut(s)	100, 186, 1065
Cfr10I	RCCGGY	1 cut(s)	56
Cfr13I	GGNCC	2 cut(s)	195, 386
Csp6I	GTAC	5 cut(s)	564, 576, 791, 944, 1380
CviAII	CATG	9 cut(s)	539, 770, 950, 1136, 1339, 1347, 1408, 1648, 1653
CviQI	GTAC	5 cut(s)	564, 576, 791, 944, 1380
DdeI	CTNAG	8 cut(s)	52, 294, 429, 681, 747, 1254, 1483, 1593
DpnI	GATC	6 cut(s)	285, 327, 364, 421, 543, 1252
DpnII	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
DrdI	GACNNNNNNGTC	1 cut(s)	1736
DseDI	GACNNNNNNGTC	1 cut(s)	1736
EaeI	YGGCCR	1 cut(s)	1294
Eam1104I	CTCTTC	1 cut(s)	1365
EarI	CTCTTC	1 cut(s)	1365
Eco24I	GRGCYC	1 cut(s)	182
Eco31I	GGTCTC	3 cut(s)	379, 677, 1486
Eco32I	GATATC	2 cut(s)	334, 1755
Eco47I	GGWCC	1 cut(s)	386
Eco47III	AGCGCT	1 cut(s)	99
Eco57I	CTGAAG	3 cut(s)	114, 585, 1389
Eco72I	CACGTG	1 cut(s)	263
Eco88I	CYCGRG	4 cut(s)	198, 692, 1225, 1383
Eco91I	GGTNACC	1 cut(s)	1199
EcoNI	CCTNNNNNAGG	1 cut(s)	875
EcoO65I	GGTNACC	1 cut(s)	1199
EcoRI	GAATTC	2 cut(s)	736, 1215
EcoRII	CCWGG	2 cut(s)	927, 1070
EcoRV	GATATC	2 cut(s)	334, 1755
EcoT22I	ATGCAT	1 cut(s)	1054
EcoT38I	GRGCYC	1 cut(s)	182
FaeI	CATG	9 cut(s)	542, 773, 953, 1139, 1342, 1350, 1411, 1651, 1656
FaqI	GGGAC	3 cut(s)	675, 1299, 1724
FatI	CATG	9 cut(s)	538, 769, 949, 1135, 1338, 1346, 1407, 1647, 1652
FauI	CCCGC	1 cut(s)	1701
FauNDI	CATATG	1 cut(s)	1583
FbaI	TGATCA	1 cut(s)	541
FblI	GTMKAC	2 cut(s)	172, 842
Fnu4HI	GCNGC	3 cut(s)	166, 1066, 1148
FokI	GGATG	3 cut(s)	1061, 1451, 1574
FriOI	GRGCYC	1 cut(s)	182
Fsp4HI	GCNGC	3 cut(s)	166, 1066, 1148
FspBI	CTAG	4 cut(s)	573, 1020, 1248, 1377
GlaI	GCGC	3 cut(s)	99, 185, 1064
GluI	GCNGC	3 cut(s)	166, 1066, 1148
GsaI	CCCAGC	1 cut(s)	1499
GsuI	CTGGAG	3 cut(s)	361, 375, 1054
HaeII	RGCGCY	1 cut(s)	101
HaeIII	GGCC	4 cut(s)	196, 927, 1281, 1296
HapII	CCGG	5 cut(s)	57, 346, 475, 1204, 1557
HhaI	GCGC	3 cut(s)	100, 186, 1065
Hin1II	CATG	9 cut(s)	542, 773, 953, 1139, 1342, 1350, 1411, 1651, 1656
Hin6I	GCGC	3 cut(s)	98, 184, 1063
HinP1I	GCGC	3 cut(s)	98, 184, 1063
HincII	GTYRAC	4 cut(s)	173, 463, 843, 1543
HindII	GTYRAC	4 cut(s)	173, 463, 843, 1543
HpaII	CCGG	5 cut(s)	57, 346, 475, 1204, 1557
HphI	GGTGA	7 cut(s)	76, 388, 1080, 1175, 1193, 1439, 1717
Hpy166II	GTNNAC	9 cut(s)	173, 386, 396, 463, 791, 843, 944, 1232, 1543
Hpy188I	TCNGA	9 cut(s)	94, 325, 430, 604, 682, 735, 832, 1255, 1750
Hpy8I	GTNNAC	9 cut(s)	173, 386, 396, 463, 791, 843, 944, 1232, 1543
Hpy99I	CGWCG	2 cut(s)	844, 1239
HpyAV	CCTTC	7 cut(s)	143, 353, 440, 881, 897, 1017, 1598
HpyCH4III	ACNGT	7 cut(s)	393, 439, 610, 1041, 1473, 1575, 1733
HpyCH4IV	ACGT	2 cut(s)	262, 780
HpyCH4V	TGCA	4 cut(s)	260, 314, 1004, 1052
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1350, 1698
HpyF3I	CTNAG	8 cut(s)	52, 294, 429, 681, 747, 1254, 1483, 1593
HpySE526I	ACGT	2 cut(s)	262, 780
Hsp92II	CATG	9 cut(s)	542, 773, 953, 1139, 1342, 1350, 1411, 1651, 1656
HspAI	GCGC	3 cut(s)	98, 184, 1063
Kpn2I	TCCGGA	1 cut(s)	345
Ksp22I	TGATCA	1 cut(s)	541
Kzo9I	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
LmnI	GCTCC	3 cut(s)	257, 1073, 1492
Lsp1109I	GCAGC	3 cut(s)	177, 1077, 1134
LweI	GCATC	3 cut(s)	991, 1039, 1508
MaeI	CTAG	4 cut(s)	573, 1020, 1248, 1377
MaeII	ACGT	2 cut(s)	262, 780
MaeIII	GTNAC	4 cut(s)	1199, 1461, 1529, 1577
MalI	GATC	6 cut(s)	285, 327, 364, 421, 543, 1252
MboI	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
MboII	GAAGA	8 cut(s)	293, 455, 591, 944, 963, 1382, 1616, 1749
MflI	RGATCY	2 cut(s)	362, 1250
MhlI	GDGCHC	3 cut(s)	182, 437, 1346
MlsI	TGGCCA	1 cut(s)	1296
MluNI	TGGCCA	1 cut(s)	1296
MlyI	GAGTC	3 cut(s)	210, 845, 1382
MmeI	TCCRAC	1 cut(s)	999
Mox20I	TGGCCA	1 cut(s)	1296
Mph1103I	ATGCAT	1 cut(s)	1054
MroI	TCCGGA	1 cut(s)	345
MroXI	GAANNNNTTC	2 cut(s)	865, 1269
MscI	TGGCCA	1 cut(s)	1296
MseI	TTAA	4 cut(s)	452, 471, 801, 1524
MslI	CAYNNNNRTG	3 cut(s)	295, 954, 1332
Msp20I	TGGCCA	1 cut(s)	1296
MspI	CCGG	5 cut(s)	57, 346, 475, 1204, 1557
MspR9I	CCNGG	5 cut(s)	476, 929, 1072, 1205, 1558
MvaI	CCWGG	2 cut(s)	929, 1072
MvnI	CGCG	1 cut(s)	184
MwoI	GCNNNNNNNGC	2 cut(s)	1350, 1698
NciI	CCSGG	3 cut(s)	476, 1205, 1558
NdeI	CATATG	1 cut(s)	1583
NdeII	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
NlaIII	CATG	9 cut(s)	542, 773, 953, 1139, 1342, 1350, 1411, 1651, 1656
NlaIV	GGNNCC	3 cut(s)	197, 253, 364
NmeAIII	GCCGAG	2 cut(s)	172, 1727
NmuCI	GTSAC	3 cut(s)	1199, 1461, 1529
NsiI	ATGCAT	1 cut(s)	1054
NspI	RCATGY	2 cut(s)	1342, 1651
OliI	CACNNNNGTG	2 cut(s)	295, 1332
PaeR7I	CTCGAG	1 cut(s)	1225
PagI	TCATGA	2 cut(s)	538, 1135
PaqCI	CACCTGC	1 cut(s)	407
PciI	ACATGT	1 cut(s)	1647
PcsI	WCGNNNNNNNCGW	1 cut(s)	739
PdmI	GAANNNNTTC	2 cut(s)	865, 1269
PfoI	TCCNGGA	1 cut(s)	1070
PkrI	GCNGC	3 cut(s)	167, 1067, 1149
PleI	GAGTC	3 cut(s)	209, 845, 1381
PmaCI	CACGTG	1 cut(s)	263
PmlI	CACGTG	1 cut(s)	263
PpsI	GAGTC	3 cut(s)	209, 845, 1381
Ppu21I	YACGTR	1 cut(s)	263
PscI	ACATGT	1 cut(s)	1647
Psp6I	CCWGG	2 cut(s)	927, 1070
PspCI	CACGTG	1 cut(s)	263
PspEI	GGTNACC	1 cut(s)	1199
PspFI	CCCAGC	1 cut(s)	1495
PspGI	CCWGG	2 cut(s)	927, 1070
PspN4I	GGNNCC	3 cut(s)	197, 253, 364
PspPI	GGNCC	2 cut(s)	195, 386
PspXI	VCTCGAGB	1 cut(s)	1225
PstNI	CAGNNNCTG	1 cut(s)	1490
PsuI	RGATCY	2 cut(s)	362, 1250
RsaI	GTAC	5 cut(s)	565, 577, 792, 945, 1381
RsaNI	GTAC	5 cut(s)	564, 576, 791, 944, 1380
RseI	CAYNNNNRTG	3 cut(s)	295, 954, 1332
SalI	GTCGAC	2 cut(s)	171, 841
SaqAI	TTAA	4 cut(s)	452, 471, 801, 1524
SatI	GCNGC	3 cut(s)	166, 1066, 1148
Sau3AI	GATC	6 cut(s)	283, 325, 362, 419, 541, 1250
Sau96I	GGNCC	2 cut(s)	195, 386
ScaI	AGTACT	3 cut(s)	565, 577, 1381
SchI	GAGTC	3 cut(s)	210, 845, 1382
ScrFI	CCNGG	5 cut(s)	476, 929, 1072, 1205, 1558
SduI	GDGCHC	3 cut(s)	182, 437, 1346
SfaNI	GCATC	3 cut(s)	991, 1039, 1508
Sfr274I	CTCGAG	1 cut(s)	1225
SinI	GGWCC	1 cut(s)	386
SlaI	CTCGAG	1 cut(s)	1225
SmiMI	CAYNNNNRTG	3 cut(s)	295, 954, 1332
SmlI	CTYRAG	1 cut(s)	1225
SmoI	CTYRAG	1 cut(s)	1225
SsiI	CCGC	4 cut(s)	213, 570, 1450, 1694
SspMI	CTAG	4 cut(s)	573, 1020, 1248, 1377
StyD4I	CCNGG	5 cut(s)	474, 927, 1070, 1203, 1556
TaaI	ACNGT	7 cut(s)	393, 439, 610, 1041, 1473, 1575, 1733
TaiI	ACGT	2 cut(s)	265, 783
TaqI	TCGA	7 cut(s)	161, 172, 842, 849, 1226, 1475, 1618
TatI	WGTACW	5 cut(s)	563, 575, 790, 943, 1379
Tru1I	TTAA	4 cut(s)	452, 471, 801, 1524
Tru9I	TTAA	4 cut(s)	452, 471, 801, 1524
TscAI	CASTG	5 cut(s)	396, 442, 463, 1476, 1580
TseFI	GTSAC	3 cut(s)	1199, 1461, 1529
TseI	GCWGC	3 cut(s)	165, 1065, 1147
Tsp45I	GTSAC	3 cut(s)	1199, 1461, 1529
TspDTI	ATGAA	5 cut(s)	17, 70, 938, 1124, 1194
TspGWI	ACGGA	2 cut(s)	828, 1698
TspRI	CASTG	5 cut(s)	396, 442, 463, 1476, 1580
VpaK11BI	GGWCC	1 cut(s)	386
XagI	CCTNNNNNAGG	1 cut(s)	875
XapI	RAATTY	4 cut(s)	11, 736, 1215, 1614
XbaI	TCTAGA	1 cut(s)	1247
XceI	RCATGY	2 cut(s)	1342, 1651
XhoI	CTCGAG	1 cut(s)	1225
XmiI	GTMKAC	2 cut(s)	172, 842
XmnI	GAANNNNTTC	2 cut(s)	865, 1269
XspI	CTAG	4 cut(s)	573, 1020, 1248, 1377
ZrmI	AGTACT	3 cut(s)	565, 577, 1381
Zsp2I	ATGCAT	1 cut(s)	1054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.