Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010512

Physical Location & Seq

Genomic Coordinates Reverse (-)

11989187 .. 11993349

Gene Structure 4163 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1160200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGGTTCGAGCAGCAGTCGACTGGGCTCGCGCCCATCTCGGCCCCGAGTCAATCGCACCGCTAGTCGTTCCATACTCTCCTCTTTCGTCTGCGGTGGCTCCTCCGCAGGTGCTACTCAAGAGATGGAAGATCACGCAGCTGTGTGTCTTGTGAAATGTGCAGAGCAATGTGATCGTGATATCAATCAAGTCCAGAACTCCGGAGAGGGAACCTTGTTGGTCTCCAGTACTGGATCACCGTTCACCCGCCCCGTTATTGAAATTGGATCATCATCTGAGCACACTGTTGAAGAAGGTTTACGCGGTGTTGACACAAGTAACCGGGGGAAGTGCTTGTCCAAAAGTAAAGAACTAATACCTCATCAGGTAAGTGCTGATTATAGTCACGACCAATCTTGTATGGATAATAGTACTACAGCTAGTGCTTCGTTTAGACAACAACAATCTTCAGAAACAGTCTCTTTGAATCGTTCGGTTAATGTGGATGCAGTCAATGGCATTGACAATCCAGCAGACAAGAATGTATCTCAGACCTGTCCCGAGGTTATGTGTCCAAGTAGTTCATCTCTACAAGGGATGCAGAATTCCCGTTCTCAGGAAGTGTCTGTTGAGAGTCATGTGGCTGACGTAAGGGGTGTGCAAAGTTCTAATGCTGATACTGATTCTGCTAATGTTTCTGAAATTCCATCATCTTTTGACTCACTTGGAAATGATTCTCTAAGGGAGGCAATACCTTCTGGTTTAGGTTTCCTTGTGTCCAATAGGGAACAAGGCCAGGAAGATGGAAGTGTACTTCATGTTGATGTTATGAGCATTTCTTCCAACTTTTTGAGGAGTAGCAATGGTGATGCAAGTAATCGTGAGGCTAGAAGGAATGGCAGACGACTGTTCTGGGATGCATTTTCAAGGCGCAGCTCCAGGAGATTCATTGATTCACCAACCATTCTTTTCTCTACTGATGATACTGAGGATATGGGATTTCATGACAGATGGCTACTTGGTTTGAGTGGTGATTTATTTGATGATGGGATTGGAGCTGATTCTGGTCACCGGGCCAGTAGAATTCCCTACTCGAATGAACGACGACGACATTCTAGATCTGAGGTCTGGGAAAGACTTCGTGTTGGCCTTGATGATAACGGCCAGCGTAATACCTCTTGTCCCACAGGAATCCACCCTCGTGGCATGTGCTCATGTGGCTCATTCTCGACGACCGAAGAGTCTAGTACTCGGGGAAGTATATCAAGAATTGTCATGCTCGCTGAAGCTCTATTTGAGGTTTTGGATGAAATTCACCGCCAACCTGTGTCACTTTCACTATCGATGGTCTCGGCTCCTGCCCCAGCATCTGTTGTTGATGGTTTTCCTCTTAAAAGTCACACAAAGGTTGACAAATCAAAGTCCGGGGATGATGTTGAGCAGTGTTACATATGCCTCGCTGAGTATGAGGAAGGAGACAACATTCGAGTTCTTCCCTGCCATCACGAGTATCACATGTCCTGCGTTGACAAGTGGCTCAAAGAGATACACGGGGTTTGCCCACTTTGCCGAGGAGATGTTCGTGAGGGTTTCACCGACTGTTCTGTCTCTAACTCAGAGATATCTTCTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000578 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0007389 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009798 GO:0009880 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048856 GO:0051603 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_11 zf-RING_2

Protein Analysis

538

Amino Acids

58.36

Weight (kDa)

5.28

Isoelectric Point (pI)

61.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	473 - 517	4.1e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	474 - 517	2e-12	Ring finger domain
zf-RING_11	PF17123	474 - 502	1.7e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	474 - 516	5.5e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	475 - 516	1.9e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010512)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g01770
malus_domestica	MD02G1013400.v1.1 MD15G1160200.v1.1
prunus_persica	Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1
pyrus_communis	pycom02g01250 pycom15g14410
rosa_chinensis	RchiOBHm_Chr2g0086731
rosa_laevigata	RLG00000015786
rosa_multiflora	Rmu_sc0000335.1_g000009 Rmu_sc0004626.1_g000001 Rmu_sc0030160.1_g000001 Rmu_sc0035211.1_g000001
rosa_roxburghii	Rroxscaffold_2G00154240
rosa_rugosa	Rorug01G0469200
rosa_samantha	Rh2AG021300 Rh2BG021200 Rh2CG022100 Rh2DG021900
rosa_wichuraiana	Rw2G001730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	98
AasI	GACNNNNNNGTC	2 cut(s)	1219, 1583
Acc36I	ACCTGC	1 cut(s)	98
AccI	GTMKAC	1 cut(s)	19
AccII	CGCG	2 cut(s)	31, 303
AccIII	TCCGGA	1 cut(s)	200
AciI	CCGC	6 cut(s)	60, 93, 105, 247, 303, 1297
AclWI	GGATC	2 cut(s)	241, 274
AcoI	YGGCCR	1 cut(s)	1141
AcsI	RAATTY	4 cut(s)	583, 681, 1062, 1290
AcuI	CTGAAG	2 cut(s)	432, 1284
AfaI	GTAC	4 cut(s)	229, 412, 792, 1228
AfiI	CCNNNNNNNGG	1 cut(s)	595
AflIII	ACRYGT	1 cut(s)	1494
AgsI	TTSAA	4 cut(s)	260, 290, 466, 906
AjnI	CCWGG	2 cut(s)	774, 917
AleI	CACNNNNGTG	1 cut(s)	1179
AluBI	AGCT	5 cut(s)	140, 419, 915, 1037, 1268
AluI	AGCT	5 cut(s)	140, 419, 915, 1037, 1268
Alw21I	GWGCWC	2 cut(s)	282, 1193
Alw26I	GTCTC	5 cut(s)	226, 463, 1333, 1449, 1591
AlwI	GGATC	2 cut(s)	241, 274
AlwNI	CAGNNNCTG	1 cut(s)	1349
Ama87I	CYCGRG	3 cut(s)	45, 539, 1230
Aor13HI	TCCGGA	1 cut(s)	200
AoxI	GGCC	5 cut(s)	41, 772, 1053, 1126, 1141
ApeKI	GCWGC	3 cut(s)	12, 137, 912
ApoI	RAATTY	4 cut(s)	583, 681, 1062, 1290
Asp700I	GAANNNNTTC	2 cut(s)	712, 1116
AspLEI	GCGC	2 cut(s)	33, 912
AspS9I	GGNCC	2 cut(s)	42, 1053
AsuC2I	CCSGG	3 cut(s)	323, 1052, 1405
AsuHPI	GGTGA	8 cut(s)	228, 235, 857, 927, 1022, 1040, 1286, 1564
AvaI	CYCGRG	3 cut(s)	45, 539, 1230
BanII	GRGCYC	1 cut(s)	29
BarI	GAAGNNNNNNTAC	2 cut(s)	282, 314
BauI	CACGAG	2 cut(s)	1179, 1484
Bbv12I	GWGCWC	2 cut(s)	282, 1193
BbvI	GCAGC	3 cut(s)	24, 149, 924
BccI	CCATC	9 cut(s)	43, 118, 694, 776, 984, 1019, 1318, 1352, 1488
BceAI	ACGGC	1 cut(s)	1156
BcgI	CGANNNNNNTGC	2 cut(s)	155, 189
BciT130I	CCWGG	2 cut(s)	776, 919
BcnI	CCSGG	3 cut(s)	323, 1052, 1405
BcoDI	GTCTC	5 cut(s)	226, 463, 1333, 1449, 1591
BfaI	CTAG	5 cut(s)	63, 420, 867, 1095, 1224
BfmI	CTRYAG	1 cut(s)	414
BfuAI	ACCTGC	1 cut(s)	98
BglII	AGATCT	1 cut(s)	1097
BisI	GCNGC	3 cut(s)	13, 138, 913
BlsI	GCNGC	3 cut(s)	14, 139, 914
BmcAI	AGTACT	3 cut(s)	229, 412, 1228
Bme1390I	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
BmeT110I	CYCGRG	3 cut(s)	45, 539, 1230
BmgT120I	GGNCC	2 cut(s)	42, 1053
BmiI	GGNNCC	4 cut(s)	44, 100, 211, 1335
BmrFI	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
BmrI	ACTGGG	1 cut(s)	32
BmsI	GCATC	5 cut(s)	475, 567, 838, 886, 1355
BmuI	ACTGGG	1 cut(s)	32
BpmI	CTGGAG	2 cut(s)	208, 901
BpuEI	CTTGAG	1 cut(s)	102
BpuMI	CCSGG	3 cut(s)	323, 1052, 1405
Bsa29I	ATCGAT	1 cut(s)	1322
BsaBI	GATNNNNATC	2 cut(s)	183, 271
BsaI	GGTCTC	2 cut(s)	226, 1333
BsaJI	CCNNGG	4 cut(s)	322, 540, 1404, 1549
BsaWI	WCCGGW	1 cut(s)	200
Bsc4I	CCNNNNNNNGG	1 cut(s)	595
Bse1I	ACTGG	4 cut(s)	27, 225, 235, 1056
Bse3DI	GCAATG	2 cut(s)	173, 847
Bse8I	GATNNNNATC	2 cut(s)	183, 271
BseAI	TCCGGA	1 cut(s)	200
BseBI	CCWGG	2 cut(s)	776, 919
BseCI	ATCGAT	1 cut(s)	1322
BseDI	CCNNGG	4 cut(s)	322, 540, 1404, 1549
BseGI	GGATG	5 cut(s)	490, 582, 901, 1291, 1414
BseJI	GATNNNNATC	2 cut(s)	183, 271
BseLI	CCNNNNNNNGG	1 cut(s)	595
BseMI	GCAATG	2 cut(s)	173, 847
BseMII	CTCAG	7 cut(s)	267, 542, 608, 957, 1092, 1431, 1608
BseNI	ACTGG	4 cut(s)	27, 225, 235, 1056
BseRI	GAGGAG	4 cut(s)	70, 91, 847, 1566
BseXI	GCAGC	3 cut(s)	24, 149, 924
BseYI	CCCAGC	1 cut(s)	1342
BsgI	GTGCAG	1 cut(s)	180
Bsh1236I	CGCG	2 cut(s)	31, 303
Bsh1285I	CGRYCG	1 cut(s)	1215
BshFI	GGCC	5 cut(s)	43, 774, 1055, 1128, 1143
BshVI	ATCGAT	1 cut(s)	1322
BsiEI	CGRYCG	1 cut(s)	1215
BsiHKAI	GWGCWC	2 cut(s)	282, 1193
BsiHKCI	CYCGRG	3 cut(s)	45, 539, 1230
BsiSI	CCGG	4 cut(s)	201, 322, 1051, 1404
BslFI	GGGAC	2 cut(s)	522, 1146
BslI	CCNNNNNNNGG	1 cut(s)	595
BsmAI	GTCTC	5 cut(s)	226, 463, 1333, 1449, 1591
BsmFI	GGGAC	2 cut(s)	522, 1146
BsnI	GGCC	5 cut(s)	43, 774, 1055, 1128, 1143
Bso31I	GGTCTC	2 cut(s)	226, 1333
BsoBI	CYCGRG	3 cut(s)	45, 539, 1230
Bsp1286I	GDGCHC	3 cut(s)	29, 282, 1193
Bsp13I	TCCGGA	1 cut(s)	200
Bsp143I	GATC	5 cut(s)	130, 172, 233, 266, 1097
BspACI	CCGC	6 cut(s)	60, 93, 105, 247, 303, 1297
BspANI	GGCC	5 cut(s)	43, 774, 1055, 1128, 1143
BspCNI	CTCAG	7 cut(s)	268, 541, 607, 958, 1093, 1432, 1607
BspDI	ATCGAT	1 cut(s)	1322
BspEI	TCCGGA	1 cut(s)	200
BspFNI	CGCG	2 cut(s)	31, 303
BspHI	TCATGA	1 cut(s)	982
BspLI	GGNNCC	4 cut(s)	44, 100, 211, 1335
BspMI	ACCTGC	1 cut(s)	98
BspPI	GGATC	2 cut(s)	241, 274
BspTNI	GGTCTC	2 cut(s)	226, 1333
BsrDI	GCAATG	2 cut(s)	173, 847
BsrI	ACTGG	4 cut(s)	27, 225, 235, 1056
BssECI	CCNNGG	4 cut(s)	322, 540, 1404, 1549
BssMI	GATC	5 cut(s)	130, 172, 233, 266, 1097
BssSI	CACGAG	2 cut(s)	1179, 1484
Bst2BI	CACGAG	2 cut(s)	1179, 1484
Bst2UI	CCWGG	2 cut(s)	776, 919
Bst4CI	ACNGT	5 cut(s)	240, 286, 457, 888, 1580
Bst6I	CTCTTC	1 cut(s)	1212
BstC8I	GCNNGC	3 cut(s)	29, 1145, 1260
BstDEI	CTNAG	8 cut(s)	276, 528, 594, 719, 966, 1101, 1440, 1594
BstEII	GGTNACC	1 cut(s)	1046
BstF5I	GGATG	5 cut(s)	490, 582, 901, 1291, 1414
BstFNI	CGCG	2 cut(s)	31, 303
BstHHI	GCGC	2 cut(s)	33, 912
BstKTI	GATC	5 cut(s)	133, 175, 236, 269, 1100
BstMAI	GTCTC	5 cut(s)	226, 463, 1333, 1449, 1591
BstMBI	GATC	5 cut(s)	130, 172, 233, 266, 1097
BstMCI	CGRYCG	1 cut(s)	1215
BstMWI	GCNNNNNNNGC	2 cut(s)	1197, 1545
BstNI	CCWGG	2 cut(s)	776, 919
BstNSI	RCATGY	2 cut(s)	1189, 1498
BstPI	GGTNACC	1 cut(s)	1046
BstSCI	CCNGG	5 cut(s)	321, 774, 917, 1050, 1403
BstSFI	CTRYAG	1 cut(s)	414
BstUI	CGCG	2 cut(s)	31, 303
BstV1I	GCAGC	3 cut(s)	24, 149, 924
BstX2I	RGATCY	1 cut(s)	1097
BstXI	CCANNNNNNTGG	2 cut(s)	782, 1181
BstYI	RGATCY	1 cut(s)	1097
Bsu15I	ATCGAT	1 cut(s)	1322
BsuRI	GGCC	5 cut(s)	43, 774, 1055, 1128, 1143
BsuTUI	ATCGAT	1 cut(s)	1322
BtsCI	GGATG	5 cut(s)	490, 582, 901, 1291, 1414
BtsI	GCAGTG	1 cut(s)	1427
BtsIMutI	CAGTG	2 cut(s)	282, 1427
BveI	ACCTGC	1 cut(s)	98
Cac8I	GCNNGC	3 cut(s)	29, 1145, 1260
CaiI	CAGNNNCTG	1 cut(s)	1349
CciI	TCATGA	1 cut(s)	982
CfoI	GCGC	2 cut(s)	33, 912
Cfr13I	GGNCC	2 cut(s)	42, 1053
ClaI	ATCGAT	1 cut(s)	1322
Csp6I	GTAC	4 cut(s)	228, 411, 791, 1227
CviAII	CATG	7 cut(s)	617, 797, 983, 1186, 1194, 1255, 1495
CviQI	GTAC	4 cut(s)	228, 411, 791, 1227
DdeI	CTNAG	8 cut(s)	276, 528, 594, 719, 966, 1101, 1440, 1594
DpnI	GATC	5 cut(s)	132, 174, 235, 268, 1099
DpnII	GATC	5 cut(s)	130, 172, 233, 266, 1097
DrdI	GACNNNNNNGTC	2 cut(s)	1219, 1583
DseDI	GACNNNNNNGTC	2 cut(s)	1219, 1583
EaeI	YGGCCR	1 cut(s)	1141
Eam1104I	CTCTTC	1 cut(s)	1212
EarI	CTCTTC	1 cut(s)	1212
Eco24I	GRGCYC	1 cut(s)	29
Eco31I	GGTCTC	2 cut(s)	226, 1333
Eco32I	GATATC	2 cut(s)	181, 1602
Eco57I	CTGAAG	2 cut(s)	432, 1284
Eco88I	CYCGRG	3 cut(s)	45, 539, 1230
Eco91I	GGTNACC	1 cut(s)	1046
EcoO65I	GGTNACC	1 cut(s)	1046
EcoRI	GAATTC	2 cut(s)	583, 1062
EcoRII	CCWGG	2 cut(s)	774, 917
EcoRV	GATATC	2 cut(s)	181, 1602
EcoT22I	ATGCAT	1 cut(s)	901
EcoT38I	GRGCYC	1 cut(s)	29
FaeI	CATG	7 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498
FaqI	GGGAC	2 cut(s)	522, 1146
FatI	CATG	7 cut(s)	616, 796, 982, 1185, 1193, 1254, 1494
FauI	CCCGC	1 cut(s)	254
FauNDI	CATATG	1 cut(s)	1430
FblI	GTMKAC	1 cut(s)	19
Fnu4HI	GCNGC	3 cut(s)	13, 138, 913
FokI	GGATG	5 cut(s)	497, 589, 908, 1298, 1421
FriOI	GRGCYC	1 cut(s)	29
Fsp4HI	GCNGC	3 cut(s)	13, 138, 913
FspBI	CTAG	5 cut(s)	63, 420, 867, 1095, 1224
GlaI	GCGC	2 cut(s)	32, 911
GluI	GCNGC	3 cut(s)	13, 138, 913
GsaI	CCCAGC	1 cut(s)	1346
GsuI	CTGGAG	2 cut(s)	208, 901
HaeIII	GGCC	5 cut(s)	43, 774, 1055, 1128, 1143
HapII	CCGG	4 cut(s)	201, 322, 1051, 1404
HhaI	GCGC	2 cut(s)	33, 912
Hin1II	CATG	7 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498
Hin6I	GCGC	2 cut(s)	31, 910
HinP1I	GCGC	2 cut(s)	31, 910
HincII	GTYRAC	4 cut(s)	20, 310, 1390, 1507
HindII	GTYRAC	4 cut(s)	20, 310, 1390, 1507
HpaII	CCGG	4 cut(s)	201, 322, 1051, 1404
HphI	GGTGA	8 cut(s)	228, 235, 857, 927, 1022, 1040, 1286, 1564
Hpy166II	GTNNAC	7 cut(s)	20, 243, 299, 310, 791, 1390, 1507
Hpy188I	TCNGA	6 cut(s)	277, 451, 531, 679, 1102, 1597
Hpy8I	GTNNAC	7 cut(s)	20, 243, 299, 310, 791, 1390, 1507
Hpy99I	CGWCG	3 cut(s)	1086, 1089, 1213
HpyAV	CCTTC	4 cut(s)	287, 744, 864, 1445
HpyCH4III	ACNGT	5 cut(s)	240, 286, 457, 888, 1580
HpyCH4IV	ACGT	1 cut(s)	627
HpyCH4V	TGCA	6 cut(s)	161, 488, 580, 640, 851, 899
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1197, 1545
HpyF3I	CTNAG	8 cut(s)	276, 528, 594, 719, 966, 1101, 1440, 1594
HpySE526I	ACGT	1 cut(s)	627
Hsp92II	CATG	7 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498
HspAI	GCGC	2 cut(s)	31, 910
Kpn2I	TCCGGA	1 cut(s)	200
Kzo9I	GATC	5 cut(s)	130, 172, 233, 266, 1097
LmnI	GCTCC	4 cut(s)	104, 920, 1034, 1339
Lsp1109I	GCAGC	3 cut(s)	24, 149, 924
LweI	GCATC	5 cut(s)	475, 567, 838, 886, 1355
MaeI	CTAG	5 cut(s)	63, 420, 867, 1095, 1224
MaeII	ACGT	1 cut(s)	627
MaeIII	GTNAC	6 cut(s)	317, 383, 1046, 1308, 1376, 1424
MalI	GATC	5 cut(s)	132, 174, 235, 268, 1099
MboI	GATC	5 cut(s)	130, 172, 233, 266, 1097
MboII	GAAGA	8 cut(s)	140, 302, 438, 791, 810, 1229, 1463, 1596
MflI	RGATCY	1 cut(s)	1097
MhlI	GDGCHC	3 cut(s)	29, 282, 1193
MluCI	AATT	6 cut(s)	261, 583, 681, 1062, 1248, 1290
MlyI	GAGTC	4 cut(s)	57, 622, 692, 1229
MmeI	TCCRAC	1 cut(s)	846
Mph1103I	ATGCAT	1 cut(s)	901
MroI	TCCGGA	1 cut(s)	200
MroXI	GAANNNNTTC	2 cut(s)	712, 1116
MseI	TTAA	2 cut(s)	477, 1371
MslI	CAYNNNNRTG	2 cut(s)	801, 1179
MspA1I	CMGCKG	1 cut(s)	140
MspI	CCGG	4 cut(s)	201, 322, 1051, 1404
MspR9I	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
MvaI	CCWGG	2 cut(s)	776, 919
MvnI	CGCG	2 cut(s)	31, 303
MwoI	GCNNNNNNNGC	2 cut(s)	1197, 1545
NciI	CCSGG	3 cut(s)	323, 1052, 1405
NdeI	CATATG	1 cut(s)	1430
NdeII	GATC	5 cut(s)	130, 172, 233, 266, 1097
NlaIII	CATG	7 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498
NlaIV	GGNNCC	4 cut(s)	44, 100, 211, 1335
NmeAIII	GCCGAG	3 cut(s)	19, 1310, 1574
NmuCI	GTSAC	4 cut(s)	383, 1046, 1308, 1376
NsiI	ATGCAT	1 cut(s)	901
NspI	RCATGY	2 cut(s)	1189, 1498
OliI	CACNNNNGTG	1 cut(s)	1179
PagI	TCATGA	1 cut(s)	982
PaqCI	CACCTGC	1 cut(s)	98
PciI	ACATGT	1 cut(s)	1494
PdmI	GAANNNNTTC	2 cut(s)	712, 1116
PfeI	GAWTC	7 cut(s)	466, 662, 713, 924, 932, 1040, 1170
PfoI	TCCNGGA	1 cut(s)	917
PkrI	GCNGC	3 cut(s)	14, 139, 914
PleI	GAGTC	4 cut(s)	56, 621, 692, 1228
PpsI	GAGTC	4 cut(s)	56, 621, 692, 1228
PscI	ACATGT	1 cut(s)	1494
Psp6I	CCWGG	2 cut(s)	774, 917
PspEI	GGTNACC	1 cut(s)	1046
PspFI	CCCAGC	1 cut(s)	1342
PspGI	CCWGG	2 cut(s)	774, 917
PspN4I	GGNNCC	4 cut(s)	44, 100, 211, 1335
PspPI	GGNCC	2 cut(s)	42, 1053
PstNI	CAGNNNCTG	1 cut(s)	1349
PsuI	RGATCY	1 cut(s)	1097
PvuII	CAGCTG	1 cut(s)	140
RsaI	GTAC	4 cut(s)	229, 412, 792, 1228
RsaNI	GTAC	4 cut(s)	228, 411, 791, 1227
RseI	CAYNNNNRTG	2 cut(s)	801, 1179
SalI	GTCGAC	1 cut(s)	18
SaqAI	TTAA	2 cut(s)	477, 1371
SatI	GCNGC	3 cut(s)	13, 138, 913
Sau3AI	GATC	5 cut(s)	130, 172, 233, 266, 1097
Sau96I	GGNCC	2 cut(s)	42, 1053
ScaI	AGTACT	3 cut(s)	229, 412, 1228
SchI	GAGTC	4 cut(s)	57, 622, 692, 1229
ScrFI	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
SduI	GDGCHC	3 cut(s)	29, 282, 1193
SfaNI	GCATC	5 cut(s)	475, 567, 838, 886, 1355
SfcI	CTRYAG	1 cut(s)	414
SmiMI	CAYNNNNRTG	2 cut(s)	801, 1179
SmlI	CTYRAG	1 cut(s)	117
SmoI	CTYRAG	1 cut(s)	117
Sse9I	AATT	6 cut(s)	261, 583, 681, 1062, 1248, 1290
SsiI	CCGC	6 cut(s)	60, 93, 105, 247, 303, 1297
SspMI	CTAG	5 cut(s)	63, 420, 867, 1095, 1224
StyD4I	CCNGG	5 cut(s)	321, 774, 917, 1050, 1403
TaaI	ACNGT	5 cut(s)	240, 286, 457, 888, 1580
TaiI	ACGT	1 cut(s)	630
TaqI	TCGA	6 cut(s)	8, 19, 1073, 1208, 1322, 1465
TaqII	GACCGA	1 cut(s)	1229
TasI	AATT	6 cut(s)	261, 583, 681, 1062, 1248, 1290
TatI	WGTACW	4 cut(s)	227, 410, 790, 1226
TfiI	GAWTC	7 cut(s)	466, 662, 713, 924, 932, 1040, 1170
Tru1I	TTAA	2 cut(s)	477, 1371
Tru9I	TTAA	2 cut(s)	477, 1371
TscAI	CASTG	2 cut(s)	289, 1427
TseFI	GTSAC	4 cut(s)	383, 1046, 1308, 1376
TseI	GCWGC	3 cut(s)	12, 137, 912
Tsp45I	GTSAC	4 cut(s)	383, 1046, 1308, 1376
TspDTI	ATGAA	6 cut(s)	552, 785, 916, 971, 1092, 1302
TspRI	CASTG	2 cut(s)	289, 1427
XapI	RAATTY	4 cut(s)	583, 681, 1062, 1290
XbaI	TCTAGA	1 cut(s)	1094
XceI	RCATGY	2 cut(s)	1189, 1498
XmiI	GTMKAC	1 cut(s)	19
XmnI	GAANNNNTTC	2 cut(s)	712, 1116
XspI	CTAG	5 cut(s)	63, 420, 867, 1095, 1224
ZrmI	AGTACT	3 cut(s)	229, 412, 1228
Zsp2I	ATGCAT	1 cut(s)	901

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.