Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0010512

Physical Location & Seq

Genomic Coordinates Reverse (-)

708486 .. 712483

Gene Structure 3998 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g01250.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGGTTCGAGCAGCAGTCGACTGGGCTCGCGCCCATCTCGGGCCCGAGTCGATCGCACCGCCAGTCGTTCCATACTCTCCTCTTTCGTCTGTGGTGGCTCCTCTGCACGTGCTACTCACGAGATGGAAGATCACGCACCTGAGTGTCTTGTGAAATATGCAGAGCAATCTGATCTTGATATCAATCAAGTCCGGAGCTCTGGAGAAGGAACCTCGTTAGTCTCCAGTAGTGGACCACAGTTCACCTGCCCCATTATTGAAATTGGATCGTCATCTGAGAGCACTGTTGAAGAAGGATTAAACAGTGTTGACACAAGTAACCAGGGGAAGTGCTTGTCCAAAAGTAAAGAACTAATACCTCATCAGGTAAGTGCCGATTATAGTCACGATCAATCTTGTAGGGATAATAGTACTACCGCTAGTACTTCGTTTAGACAAGAACAATCTTCAGAAACAGTCTCTTTGAATGGTTCTGCTAATGTGGATTCAGTCAATGGCATTGACATTTCAGCAGACAAGAATGTATCTGAGAGCTGTCCCGAGGTTATGCGTCCAAGTAGTTCATCTCCACAAGGGATTCGGAATTCCCGTTCTCAGGAAGTGTCTGTTGAGAATCATGTGGCTGACGTAAGGGGTGTACAGGGTTTTAATGCTGATACTGATTCTGCTAATGTTTCTGAAACTCCGTCATCTTTCGACTCACTTGGAAATGATTCCCTTCGGGAGGCAATACCTTCTGGTTTAGGATTCCTTGTGTCCAATAGGGAACAAGGCCAGGAAGATGGAAGTGTACTTCATGTTGATGTTATGAGCATTTCTTCCAACTTATTGAGGAGAAGCAATGCTGATGCAAGTAATCGTGAGGCTAGAAGGAATGGCACGCGACTGTTCTGGGATGCATTTTCAAGGCGCAGCCCCAGGAGGTTTATTGATTCACCAACCATTCTTTTCTCTACTGATGATACTGAGGATATGGGATTTCATGACAGATGGCTGCTTGGTTTGAGTGGTGATTTCTTTGATGATGAAATTGGAGTTGATTCTGGTCACCGGGCCAATAGAATTCCCAACTCGAGTGAACGACGGCGACATTCTAGATCTGAGGTCTGGGAAAGACTTCGTGTTGGCCTTGATGATAATGGCCAGCGTAATACCTCTTGTCCCACAGGAATCCACCCTCGTGGCATGTGCTCATGTGGCTCATTCTTGATGGCTGAAGAGTCTAGTACTCGGGCAAGTATATCAAGAATTGTCATGCTCGCTGAAGCTCTATTTGAGGTTTTGGATGAAATTCACCGCCAACCTGTGTCGCTTTCACTATCGATGGTCTCAGCTCCTGCCCCAGCATCTCTTGTTGATGGTTTTCCTCTTAAAAGTCACACAAAGGTTGACAAATCAAAGTCCGGGGATGATGTTGAACAGTGTTACATATGCCTCGCTGAGTACGAGGAAGGGGACAAAATTCGAGTTCTTCCTTGCCATCACGAGTATCACATGTCATGCGTTGATAAGTGGCTCAAAGAGATACACGGAGTTTGCCCGCTTTGCCGAGGAGATGTTCGTGAGGGTTTCACCGACTGTTCTGTCTCTAACTCGGAGATATCTTCTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000578 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0007389 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009798 GO:0009880 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048856 GO:0051603 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_11 zf-RING_2

Protein Analysis

538

Amino Acids

58.54

Weight (kDa)

5.12

Isoelectric Point (pI)

61.76

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010512)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g01770
malus_domestica	MD02G1013400.v1.1 MD15G1160200.v1.1
prunus_persica	Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1 Prupe.7G255200_v2.0.a1
pyrus_communis	pycom02g01250 pycom15g14410
rosa_chinensis	RchiOBHm_Chr2g0086731
rosa_laevigata	RLG00000015786
rosa_multiflora	Rmu_sc0000335.1_g000009 Rmu_sc0004626.1_g000001 Rmu_sc0030160.1_g000001 Rmu_sc0035211.1_g000001
rosa_roxburghii	Rroxscaffold_2G00154240
rosa_rugosa	Rorug01G0469200
rosa_samantha	Rh2AG021300 Rh2BG021200 Rh2CG022100 Rh2DG021900
rosa_wichuraiana	Rw2G001730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	254
AasI	GACNNNNNNGTC	1 cut(s)	1583
Acc36I	ACCTGC	1 cut(s)	254
AccI	GTMKAC	1 cut(s)	19
AccII	CGCG	2 cut(s)	31, 883
AccIII	TCCGGA	1 cut(s)	192
AciI	CCGC	4 cut(s)	60, 417, 1297, 1541
AclWI	GGATC	1 cut(s)	274
AcoI	YGGCCR	1 cut(s)	1141
AcsI	RAATTY	4 cut(s)	583, 1062, 1290, 1461
AcuI	CTGAAG	3 cut(s)	432, 1236, 1284
AcvI	CACGTG	1 cut(s)	110
AfaI	GTAC	6 cut(s)	412, 424, 639, 792, 1228, 1445
AfiI	CCNNNNNNNGG	3 cut(s)	40, 595, 724
AflIII	ACRYGT	1 cut(s)	1494
AgsI	TTSAA	5 cut(s)	260, 290, 466, 906, 1418
AjnI	CCWGG	3 cut(s)	321, 774, 917
AleI	CACNNNNGTG	2 cut(s)	142, 1179
AloI	GAACNNNNNNTCC	2 cut(s)	574, 606
AluBI	AGCT	4 cut(s)	198, 534, 1268, 1334
AluI	AGCT	4 cut(s)	198, 534, 1268, 1334
Alw21I	GWGCWC	3 cut(s)	200, 284, 1193
Alw26I	GTCTC	4 cut(s)	226, 463, 1333, 1591
AlwI	GGATC	1 cut(s)	274
AlwNI	CAGNNNCTG	1 cut(s)	1337
Ama87I	CYCGRG	5 cut(s)	39, 45, 539, 1072, 1230
Aor13HI	TCCGGA	1 cut(s)	192
AoxI	GGCC	5 cut(s)	42, 772, 1053, 1126, 1141
ApaI	GGGCCC	1 cut(s)	46
ApeKI	GCWGC	3 cut(s)	12, 912, 994
ApoI	RAATTY	4 cut(s)	583, 1062, 1290, 1461
Asp700I	GAANNNNTTC	2 cut(s)	712, 1116
AspLEI	GCGC	2 cut(s)	33, 912
AspS9I	GGNCC	4 cut(s)	42, 43, 233, 1053
AsuC2I	CCSGG	2 cut(s)	1052, 1405
AsuHPI	GGTGA	6 cut(s)	235, 927, 1022, 1040, 1286, 1564
AvaI	CYCGRG	5 cut(s)	39, 45, 539, 1072, 1230
AvaII	GGWCC	1 cut(s)	233
BaeGI	GKGCMC	1 cut(s)	46
BalI	TGGCCA	1 cut(s)	1143
BanII	GRGCYC	3 cut(s)	29, 46, 200
BauI	CACGAG	3 cut(s)	119, 1179, 1484
BbrPI	CACGTG	1 cut(s)	110
Bbv12I	GWGCWC	3 cut(s)	200, 284, 1193
BbvI	GCAGC	3 cut(s)	24, 924, 981
BccI	CCATC	8 cut(s)	43, 118, 776, 984, 1204, 1318, 1352, 1488
BceAI	ACGGC	1 cut(s)	1100
BciT130I	CCWGG	3 cut(s)	323, 776, 919
BcnI	CCSGG	2 cut(s)	1052, 1405
BcoDI	GTCTC	4 cut(s)	226, 463, 1333, 1591
BfaI	CTAG	4 cut(s)	420, 867, 1095, 1224
BfuAI	ACCTGC	1 cut(s)	254
BglII	AGATCT	1 cut(s)	1097
BisI	GCNGC	3 cut(s)	13, 913, 995
BlsI	GCNGC	3 cut(s)	14, 914, 996
BmcAI	AGTACT	3 cut(s)	412, 424, 1228
Bme1390I	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
Bme18I	GGWCC	1 cut(s)	233
BmeT110I	CYCGRG	5 cut(s)	39, 45, 539, 1072, 1230
BmgT120I	GGNCC	4 cut(s)	42, 43, 233, 1053
BmiI	GGNNCC	3 cut(s)	44, 100, 211
BmrFI	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
BmrI	ACTGGG	1 cut(s)	32
BmsI	GCATC	3 cut(s)	838, 886, 1355
BmuI	ACTGGG	1 cut(s)	32
BpmI	CTGGAG	2 cut(s)	208, 222
BpuMI	CCSGG	2 cut(s)	1052, 1405
Bsa29I	ATCGAT	1 cut(s)	1322
BsaAI	YACGTR	1 cut(s)	110
BsaBI	GATNNNNATC	2 cut(s)	183, 271
BsaI	GGTCTC	1 cut(s)	1333
BsaJI	CCNNGG	5 cut(s)	322, 540, 917, 1404, 1549
BsaWI	WCCGGW	1 cut(s)	192
Bsc4I	CCNNNNNNNGG	3 cut(s)	40, 595, 724
Bse1I	ACTGG	3 cut(s)	27, 63, 225
Bse3DI	GCAATG	1 cut(s)	847
Bse8I	GATNNNNATC	2 cut(s)	183, 271
BseAI	TCCGGA	1 cut(s)	192
BseBI	CCWGG	3 cut(s)	323, 776, 919
BseCI	ATCGAT	1 cut(s)	1322
BseDI	CCNNGG	5 cut(s)	322, 540, 917, 1404, 1549
BseGI	GGATG	3 cut(s)	901, 1291, 1414
BseJI	GATNNNNATC	2 cut(s)	183, 271
BseLI	CCNNNNNNNGG	3 cut(s)	40, 595, 724
BseMI	GCAATG	1 cut(s)	847
BseMII	CTCAG	8 cut(s)	132, 267, 519, 608, 957, 1092, 1344, 1431
BseNI	ACTGG	3 cut(s)	27, 63, 225
BseRI	GAGGAG	4 cut(s)	70, 91, 847, 1566
BseSI	GKGCMC	1 cut(s)	46
BseXI	GCAGC	3 cut(s)	24, 924, 981
BseYI	CCCAGC	1 cut(s)	1342
BsgI	GTGCAG	1 cut(s)	90
Bsh1236I	CGCG	2 cut(s)	31, 883
Bsh1285I	CGRYCG	1 cut(s)	55
BshFI	GGCC	5 cut(s)	44, 774, 1055, 1128, 1143
BshVI	ATCGAT	1 cut(s)	1322
BsiEI	CGRYCG	1 cut(s)	55
BsiHKAI	GWGCWC	3 cut(s)	200, 284, 1193
BsiHKCI	CYCGRG	5 cut(s)	39, 45, 539, 1072, 1230
BsiSI	CCGG	3 cut(s)	193, 1051, 1404
BslFI	GGGAC	3 cut(s)	522, 1146, 1469
BslI	CCNNNNNNNGG	3 cut(s)	40, 595, 724
BsmAI	GTCTC	4 cut(s)	226, 463, 1333, 1591
BsmFI	GGGAC	3 cut(s)	522, 1146, 1469
BsnI	GGCC	5 cut(s)	44, 774, 1055, 1128, 1143
Bso31I	GGTCTC	1 cut(s)	1333
BsoBI	CYCGRG	5 cut(s)	39, 45, 539, 1072, 1230
Bsp120I	GGGCCC	1 cut(s)	42
Bsp1286I	GDGCHC	5 cut(s)	29, 46, 200, 284, 1193
Bsp13I	TCCGGA	1 cut(s)	192
Bsp1407I	TGTACA	1 cut(s)	637
Bsp143I	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
BspACI	CCGC	4 cut(s)	60, 417, 1297, 1541
BspANI	GGCC	5 cut(s)	44, 774, 1055, 1128, 1143
BspCNI	CTCAG	8 cut(s)	133, 268, 520, 607, 958, 1093, 1343, 1432
BspDI	ATCGAT	1 cut(s)	1322
BspEI	TCCGGA	1 cut(s)	192
BspFNI	CGCG	2 cut(s)	31, 883
BspHI	TCATGA	1 cut(s)	982
BspLI	GGNNCC	3 cut(s)	44, 100, 211
BspMI	ACCTGC	1 cut(s)	254
BspPI	GGATC	1 cut(s)	274
BspTNI	GGTCTC	1 cut(s)	1333
BsrDI	GCAATG	1 cut(s)	847
BsrGI	TGTACA	1 cut(s)	637
BsrI	ACTGG	3 cut(s)	27, 63, 225
BssECI	CCNNGG	5 cut(s)	322, 540, 917, 1404, 1549
BssMI	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
BssSI	CACGAG	3 cut(s)	119, 1179, 1484
Bst2BI	CACGAG	3 cut(s)	119, 1179, 1484
Bst2UI	CCWGG	3 cut(s)	323, 776, 919
Bst4CI	ACNGT	7 cut(s)	240, 286, 305, 457, 888, 1422, 1580
Bst6I	CTCTTC	1 cut(s)	1212
BstAUI	TGTACA	1 cut(s)	637
BstBAI	YACGTR	1 cut(s)	110
BstC8I	GCNNGC	5 cut(s)	29, 881, 1145, 1260, 1541
BstDEI	CTNAG	8 cut(s)	141, 276, 528, 594, 966, 1101, 1330, 1440
BstEII	GGTNACC	1 cut(s)	1046
BstENI	CCTNNNNNAGG	1 cut(s)	722
BstF5I	GGATG	3 cut(s)	901, 1291, 1414
BstFNI	CGCG	2 cut(s)	31, 883
BstHHI	GCGC	2 cut(s)	33, 912
BstKTI	GATC	6 cut(s)	55, 133, 175, 269, 391, 1100
BstMAI	GTCTC	4 cut(s)	226, 463, 1333, 1591
BstMBI	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
BstMCI	CGRYCG	1 cut(s)	55
BstMWI	GCNNNNNNNGC	2 cut(s)	1197, 1545
BstNI	CCWGG	3 cut(s)	323, 776, 919
BstNSI	RCATGY	2 cut(s)	1189, 1498
BstPI	GGTNACC	1 cut(s)	1046
BstSCI	CCNGG	5 cut(s)	321, 774, 917, 1050, 1403
BstSLI	GKGCMC	1 cut(s)	46
BstUI	CGCG	2 cut(s)	31, 883
BstV1I	GCAGC	3 cut(s)	24, 924, 981
BstX2I	RGATCY	1 cut(s)	1097
BstXI	CCANNNNNNTGG	2 cut(s)	782, 1181
BstYI	RGATCY	1 cut(s)	1097
Bsu15I	ATCGAT	1 cut(s)	1322
BsuRI	GGCC	5 cut(s)	44, 774, 1055, 1128, 1143
BsuTUI	ATCGAT	1 cut(s)	1322
BtsCI	GGATG	3 cut(s)	901, 1291, 1414
BtsIMutI	CAGTG	3 cut(s)	282, 310, 1427
BveI	ACCTGC	1 cut(s)	254
Cac8I	GCNNGC	5 cut(s)	29, 881, 1145, 1260, 1541
CaiI	CAGNNNCTG	1 cut(s)	1337
CciI	TCATGA	1 cut(s)	982
CfoI	GCGC	2 cut(s)	33, 912
Cfr13I	GGNCC	4 cut(s)	42, 43, 233, 1053
ClaI	ATCGAT	1 cut(s)	1322
CseI	GACGC	1 cut(s)	539
Csp6I	GTAC	6 cut(s)	411, 423, 638, 791, 1227, 1444
CviAII	CATG	8 cut(s)	617, 797, 983, 1186, 1194, 1255, 1495, 1500
CviQI	GTAC	6 cut(s)	411, 423, 638, 791, 1227, 1444
DdeI	CTNAG	8 cut(s)	141, 276, 528, 594, 966, 1101, 1330, 1440
DpnI	GATC	6 cut(s)	54, 132, 174, 268, 390, 1099
DpnII	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
DrdI	GACNNNNNNGTC	1 cut(s)	1583
DseDI	GACNNNNNNGTC	1 cut(s)	1583
EaeI	YGGCCR	1 cut(s)	1141
Eam1104I	CTCTTC	1 cut(s)	1212
EarI	CTCTTC	1 cut(s)	1212
Ecl136II	GAGCTC	1 cut(s)	198
Eco24I	GRGCYC	3 cut(s)	29, 46, 200
Eco31I	GGTCTC	1 cut(s)	1333
Eco32I	GATATC	2 cut(s)	181, 1602
Eco47I	GGWCC	1 cut(s)	233
Eco53kI	GAGCTC	1 cut(s)	198
Eco57I	CTGAAG	3 cut(s)	432, 1236, 1284
Eco72I	CACGTG	1 cut(s)	110
Eco88I	CYCGRG	5 cut(s)	39, 45, 539, 1072, 1230
Eco91I	GGTNACC	1 cut(s)	1046
EcoICRI	GAGCTC	1 cut(s)	198
EcoNI	CCTNNNNNAGG	1 cut(s)	722
EcoO65I	GGTNACC	1 cut(s)	1046
EcoRI	GAATTC	2 cut(s)	583, 1062
EcoRII	CCWGG	3 cut(s)	321, 774, 917
EcoRV	GATATC	2 cut(s)	181, 1602
EcoT22I	ATGCAT	1 cut(s)	901
EcoT38I	GRGCYC	3 cut(s)	29, 46, 200
FaeI	CATG	8 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498, 1503
FaqI	GGGAC	3 cut(s)	522, 1146, 1469
FatI	CATG	8 cut(s)	616, 796, 982, 1185, 1193, 1254, 1494, 1499
FauI	CCCGC	1 cut(s)	1548
FauNDI	CATATG	1 cut(s)	1430
FblI	GTMKAC	1 cut(s)	19
Fnu4HI	GCNGC	3 cut(s)	13, 913, 995
FokI	GGATG	3 cut(s)	908, 1298, 1421
FriOI	GRGCYC	3 cut(s)	29, 46, 200
Fsp4HI	GCNGC	3 cut(s)	13, 913, 995
FspBI	CTAG	4 cut(s)	420, 867, 1095, 1224
GlaI	GCGC	2 cut(s)	32, 911
GluI	GCNGC	3 cut(s)	13, 913, 995
GsaI	CCCAGC	1 cut(s)	1346
GsuI	CTGGAG	2 cut(s)	208, 222
HaeIII	GGCC	5 cut(s)	44, 774, 1055, 1128, 1143
HapII	CCGG	3 cut(s)	193, 1051, 1404
HgaI	GACGC	1 cut(s)	539
HhaI	GCGC	2 cut(s)	33, 912
Hin1II	CATG	8 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498, 1503
Hin6I	GCGC	2 cut(s)	31, 910
HinP1I	GCGC	2 cut(s)	31, 910
HincII	GTYRAC	3 cut(s)	20, 310, 1390
HindII	GTYRAC	3 cut(s)	20, 310, 1390
HpaII	CCGG	3 cut(s)	193, 1051, 1404
HphI	GGTGA	6 cut(s)	235, 927, 1022, 1040, 1286, 1564
Hpy166II	GTNNAC	8 cut(s)	20, 233, 243, 310, 638, 791, 1079, 1390
Hpy188I	TCNGA	8 cut(s)	172, 277, 451, 529, 582, 679, 1102, 1597
Hpy8I	GTNNAC	8 cut(s)	20, 233, 243, 310, 638, 791, 1079, 1390
Hpy99I	CGWCG	1 cut(s)	1086
HpyAV	CCTTC	6 cut(s)	200, 287, 728, 744, 864, 1445
HpyCH4III	ACNGT	7 cut(s)	240, 286, 305, 457, 888, 1422, 1580
HpyCH4IV	ACGT	2 cut(s)	109, 627
HpyCH4V	TGCA	4 cut(s)	107, 161, 851, 899
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1197, 1545
HpyF3I	CTNAG	8 cut(s)	141, 276, 528, 594, 966, 1101, 1330, 1440
HpySE526I	ACGT	2 cut(s)	109, 627
Hsp92II	CATG	8 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498, 1503
HspAI	GCGC	2 cut(s)	31, 910
Kpn2I	TCCGGA	1 cut(s)	192
Kzo9I	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
LmnI	GCTCC	3 cut(s)	104, 195, 1339
Lsp1109I	GCAGC	3 cut(s)	24, 924, 981
LweI	GCATC	3 cut(s)	838, 886, 1355
MaeI	CTAG	4 cut(s)	420, 867, 1095, 1224
MaeII	ACGT	2 cut(s)	109, 627
MaeIII	GTNAC	5 cut(s)	317, 383, 1046, 1376, 1424
MalI	GATC	6 cut(s)	54, 132, 174, 268, 390, 1099
MboI	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
MboII	GAAGA	8 cut(s)	140, 302, 438, 791, 810, 1229, 1463, 1596
MflI	RGATCY	1 cut(s)	1097
MhlI	GDGCHC	5 cut(s)	29, 46, 200, 284, 1193
MlsI	TGGCCA	1 cut(s)	1143
MluCI	AATT	7 cut(s)	261, 583, 1029, 1062, 1248, 1290, 1461
MluNI	TGGCCA	1 cut(s)	1143
MlyI	GAGTC	3 cut(s)	57, 692, 1229
MmeI	TCCRAC	1 cut(s)	846
Mox20I	TGGCCA	1 cut(s)	1143
Mph1103I	ATGCAT	1 cut(s)	901
MroI	TCCGGA	1 cut(s)	192
MroXI	GAANNNNTTC	2 cut(s)	712, 1116
MscI	TGGCCA	1 cut(s)	1143
MseI	TTAA	3 cut(s)	299, 648, 1371
MslI	CAYNNNNRTG	3 cut(s)	142, 801, 1179
Msp20I	TGGCCA	1 cut(s)	1143
MspI	CCGG	3 cut(s)	193, 1051, 1404
MspR9I	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
MvaI	CCWGG	3 cut(s)	323, 776, 919
MvnI	CGCG	2 cut(s)	31, 883
MwoI	GCNNNNNNNGC	2 cut(s)	1197, 1545
NciI	CCSGG	2 cut(s)	1052, 1405
NdeI	CATATG	1 cut(s)	1430
NdeII	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
NlaIII	CATG	8 cut(s)	620, 800, 986, 1189, 1197, 1258, 1498, 1503
NlaIV	GGNNCC	3 cut(s)	44, 100, 211
NmeAIII	GCCGAG	1 cut(s)	1574
NmuCI	GTSAC	3 cut(s)	383, 1046, 1376
NsiI	ATGCAT	1 cut(s)	901
NspI	RCATGY	2 cut(s)	1189, 1498
OliI	CACNNNNGTG	2 cut(s)	142, 1179
PaeR7I	CTCGAG	1 cut(s)	1072
PagI	TCATGA	1 cut(s)	982
PaqCI	CACCTGC	1 cut(s)	254
PciI	ACATGT	1 cut(s)	1494
PcsI	WCGNNNNNNNCGW	2 cut(s)	586, 1443
PdmI	GAANNNNTTC	2 cut(s)	712, 1116
PfeI	GAWTC	9 cut(s)	485, 577, 613, 662, 713, 747, 932, 1040, 1170
PkrI	GCNGC	3 cut(s)	14, 914, 996
Ple19I	CGATCG	1 cut(s)	55
PleI	GAGTC	3 cut(s)	56, 692, 1228
PmaCI	CACGTG	1 cut(s)	110
PmlI	CACGTG	1 cut(s)	110
PpsI	GAGTC	3 cut(s)	56, 692, 1228
Ppu21I	YACGTR	1 cut(s)	110
PscI	ACATGT	1 cut(s)	1494
Psp124BI	GAGCTC	1 cut(s)	200
Psp6I	CCWGG	3 cut(s)	321, 774, 917
PspCI	CACGTG	1 cut(s)	110
PspEI	GGTNACC	1 cut(s)	1046
PspFI	CCCAGC	1 cut(s)	1342
PspGI	CCWGG	3 cut(s)	321, 774, 917
PspN4I	GGNNCC	3 cut(s)	44, 100, 211
PspOMI	GGGCCC	1 cut(s)	42
PspPI	GGNCC	4 cut(s)	42, 43, 233, 1053
PspXI	VCTCGAGB	1 cut(s)	1072
PstNI	CAGNNNCTG	1 cut(s)	1337
PsuI	RGATCY	1 cut(s)	1097
PvuI	CGATCG	1 cut(s)	55
RsaI	GTAC	6 cut(s)	412, 424, 639, 792, 1228, 1445
RsaNI	GTAC	6 cut(s)	411, 423, 638, 791, 1227, 1444
RseI	CAYNNNNRTG	3 cut(s)	142, 801, 1179
SacI	GAGCTC	1 cut(s)	200
SalI	GTCGAC	1 cut(s)	18
SaqAI	TTAA	3 cut(s)	299, 648, 1371
SatI	GCNGC	3 cut(s)	13, 913, 995
Sau3AI	GATC	6 cut(s)	52, 130, 172, 266, 388, 1097
Sau96I	GGNCC	4 cut(s)	42, 43, 233, 1053
ScaI	AGTACT	3 cut(s)	412, 424, 1228
SchI	GAGTC	3 cut(s)	57, 692, 1229
ScrFI	CCNGG	5 cut(s)	323, 776, 919, 1052, 1405
SduI	GDGCHC	5 cut(s)	29, 46, 200, 284, 1193
SfaNI	GCATC	3 cut(s)	838, 886, 1355
Sfr274I	CTCGAG	1 cut(s)	1072
SinI	GGWCC	1 cut(s)	233
SlaI	CTCGAG	1 cut(s)	1072
SmiMI	CAYNNNNRTG	3 cut(s)	142, 801, 1179
SmlI	CTYRAG	1 cut(s)	1072
SmoI	CTYRAG	1 cut(s)	1072
Sse9I	AATT	7 cut(s)	261, 583, 1029, 1062, 1248, 1290, 1461
SsiI	CCGC	4 cut(s)	60, 417, 1297, 1541
SspMI	CTAG	4 cut(s)	420, 867, 1095, 1224
SstI	GAGCTC	1 cut(s)	200
StyD4I	CCNGG	5 cut(s)	321, 774, 917, 1050, 1403
TaaI	ACNGT	7 cut(s)	240, 286, 305, 457, 888, 1422, 1580
TaiI	ACGT	2 cut(s)	112, 630
TaqI	TCGA	7 cut(s)	8, 19, 51, 696, 1073, 1322, 1465
TasI	AATT	7 cut(s)	261, 583, 1029, 1062, 1248, 1290, 1461
TatI	WGTACW	5 cut(s)	410, 422, 637, 790, 1226
TfiI	GAWTC	9 cut(s)	485, 577, 613, 662, 713, 747, 932, 1040, 1170
Tru1I	TTAA	3 cut(s)	299, 648, 1371
Tru9I	TTAA	3 cut(s)	299, 648, 1371
TscAI	CASTG	3 cut(s)	289, 310, 1427
TseFI	GTSAC	3 cut(s)	383, 1046, 1376
TseI	GCWGC	3 cut(s)	12, 912, 994
Tsp45I	GTSAC	3 cut(s)	383, 1046, 1376
TspDTI	ATGAA	5 cut(s)	552, 785, 971, 1041, 1302
TspGWI	ACGGA	2 cut(s)	675, 1545
TspRI	CASTG	3 cut(s)	289, 310, 1427
VpaK11BI	GGWCC	1 cut(s)	233
XagI	CCTNNNNNAGG	1 cut(s)	722
XapI	RAATTY	4 cut(s)	583, 1062, 1290, 1461
XbaI	TCTAGA	1 cut(s)	1094
XceI	RCATGY	2 cut(s)	1189, 1498
XhoI	CTCGAG	1 cut(s)	1072
XmiI	GTMKAC	1 cut(s)	19
XmnI	GAANNNNTTC	2 cut(s)	712, 1116
XspI	CTAG	4 cut(s)	420, 867, 1095, 1224
ZrmI	AGTACT	3 cut(s)	412, 424, 1228
Zsp2I	ATGCAT	1 cut(s)	901

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.