MD02G1029900.v1.1

Equilibrative nucleotide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013473
Physical Location & Seq
Forward (+)
2314928 .. 2316448
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1029900.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGAAGGTGGAGTAAAGGGTGTTGAATATCAAGCCCAGCCAAGAGATGCTTACAAGATTGCTTACATTATTCATTTCTTGCTGGGAGCAGGCAATTTGCTTCCTTGGAATGCATTCATCACCGCCGTTGATTATTTCGGGCATCTCTATCCCACCAAGCATGTCGAGAAGGTCTTCTCTGTCGCTTACATGAGTTCTTCGGTACTCGTATTGCTTGTGATGATGACATGGAGTGGCTGGTGGGAGAAGATGAGTCCTAGGCTGAGACTCAGCTTGGGATTTTCTCTGTTTATTCTCTCGTTAATGGTGGCTCCAATTACGGACTGGCTTTCGGGCAGTTCTGCGTTGAGCAGTGGGAGAGCTAATGCAGGTTATGGCGTGACAGTTGCATCAGTTGTAGTGTGTGGCTTAGCTGATGGCTTGGTAGGAGGAAGCTTGATGGGATCCGCCGGAAAGCTTCCGAAAAAATACATGCAAGCTGTTTTTGCAGGAACCGCTTCTTCAGGTGTGATAATTTCTCTGCTGAGGATTTCAACCAAGGCGATGCTTCCACAAACCCCGAAAGGACTACAAACAAGCGCGCACTTGTACTTCATCGTCAGCACCATATTCCTCCTGTGCTGCTTGCTCGCCTGCAACTTGTTATACAGGTTGACAGTCATGCAGGAACACTGCAGGCTTGCTCAAGACGAACCTTATTGCGCAAGACCAAAATTTTGGGACGTGGCGCGAAAAGTCCCCTTGCCAGCTTTTGGGATTTTCGTAACCTATACAGTGACTCTGTCGATTTTTCCAGGATTTATTGCCGAAGATCTCGAATCCAAGCTTTTTCGAGATTGGTATCCTATTTTGCTGATCACGGTGTACAATGTTGCAGATTTAGTGGGAAAATCATTGACTGCAGTATATCTTCTAAAGAGCATTAAAAGGGCAACTTTGGCTTGCATTGCAAGGCTCCTATTTTATCCAATCTTCACTGCTTGCCTCCATGGACCGCCATGGCTGAAAACTGAAATTCCAATGGGGGTTCTCACATTTATGCTTGGATTGACCAACGGGTATCTGACAAGTGTTCTCATGATGACTGTTCCGAAAACTGTGCTGGCTTCTGAAGCGGAGCTATCTGCCATTGTCATGGTGGTGTTCCTTGGAGTTGGCTTGGTTAGCGGCTCGGTTCTCGGGTGGTTCTGGATTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nucleoside_tran

Protein Analysis

400

Amino Acids

43.67

Weight (kDa)

9.15

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 130 - 227 2.5e-20 Nucleoside transporter
Nucleoside_tran PF01733 238 - 397 3e-37 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013473)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02630 AT1G02630
fragaria_vesca FvH4_1g03060
malus_domestica MD02G1029900.v1.1 MD15G1171300.v1.1
prunus_persica Prupe.7G245000_v2.0.a1
pyrus_communis pycom02g02490 pycom15g15290
rosa_chinensis RchiOBHm_Chr2g0088191
rosa_laevigata RLG00000015930
rosa_multiflora Rmu_sc0003275.1_g000069
rosa_roxburghii Rroxscaffold_2G00152840
rosa_rugosa Rorug01G0481700
rosa_samantha Rh2AG036700 Rh2BG035900 Rh2CG037000 Rh2DG036400
rosa_wichuraiana Rw2G002890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 703
Acc36I ACCTGC 1 cut(s) 359
AccB7I CCANNNNNTGG 1 cut(s) 752
AccII CGCG 2 cut(s) 581, 730
AciI CCGC 6 cut(s) 123, 447, 495, 995, 1115, 1167
AclWI GGATC 2 cut(s) 438, 451
AcsI RAATTY 2 cut(s) 713, 1014
AcuI CTGAAG 2 cut(s) 486, 1131
AfaI GTAC 3 cut(s) 204, 590, 866
AfiI CCNNNNNNNGG 1 cut(s) 752
AgsI TTSAA 2 cut(s) 26, 534
AjiI CACGTC 1 cut(s) 724
AjnI CCWGG 1 cut(s) 793
AluBI AGCT 9 cut(s) 273, 362, 413, 435, 457, 479, 749, 826, 1120
AluI AGCT 9 cut(s) 273, 362, 413, 435, 457, 479, 749, 826, 1120
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 2 cut(s) 438, 451
Ama87I CYCGRG 1 cut(s) 1178
ApeKI GCWGC 1 cut(s) 621
ApoI RAATTY 2 cut(s) 713, 1014
ArsI GACNNNNNNTTYG 2 cut(s) 699, 731
Asp700I GAANNNNTTC 3 cut(s) 113, 173, 496
AspA2I CCTAGG 1 cut(s) 257
AspLEI GCGC 4 cut(s) 581, 583, 704, 730
AspS9I GGNCC 1 cut(s) 992
AsuHPI GGTGA 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 1178
AvaII GGWCC 1 cut(s) 992
AvrII CCTAGG 1 cut(s) 257
BamHI GGATCC 1 cut(s) 443
BbsI GAAGAC 1 cut(s) 166
BbvCI CCTCAGC 1 cut(s) 524
BbvI GCAGC 1 cut(s) 608
BccI CCATC 2 cut(s) 410, 433
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 1081, 1115
BciT130I CCWGG 1 cut(s) 795
BciVI GTATCC 1 cut(s) 852
BclI TGATCA 1 cut(s) 855
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 2 cut(s) 673, 900
BfuAI ACCTGC 1 cut(s) 359
BfuI GTATCC 1 cut(s) 852
BglII AGATCT 1 cut(s) 811
BisI GCNGC 2 cut(s) 622, 1168
BlnI CCTAGG 1 cut(s) 257
BlpI GCTNAGC 1 cut(s) 409
BlsI GCNGC 2 cut(s) 623, 1169
Bme1390I CCNGG 1 cut(s) 795
Bme18I GGWCC 1 cut(s) 992
BmeT110I CYCGRG 1 cut(s) 1178
BmgBI CACGTC 1 cut(s) 724
BmgT120I GGNCC 1 cut(s) 992
BmiI GGNNCC 4 cut(s) 312, 445, 493, 956
BmrFI CCNGG 1 cut(s) 795
BmsI GCATC 4 cut(s) 37, 151, 398, 534
BpiI GAAGAC 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 524
Bpu1102I GCTNAGC 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 669
BsaBI GATNNNNATC 1 cut(s) 840
BsaJI CCNNGG 6 cut(s) 104, 257, 537, 988, 998, 1147
Bsc4I CCNNNNNNNGG 1 cut(s) 752
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 1 cut(s) 945
Bse8I GATNNNNATC 1 cut(s) 840
BseBI CCWGG 1 cut(s) 795
BseDI CCNNGG 6 cut(s) 104, 257, 537, 988, 998, 1147
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 1 cut(s) 752
BseMI GCAATG 1 cut(s) 945
BseMII CTCAG 3 cut(s) 254, 283, 515
BseNI ACTGG 1 cut(s) 329
BsePI GCGCGC 1 cut(s) 579
BseXI GCAGC 1 cut(s) 608
BseYI CCCAGC 2 cut(s) 36, 82
Bsh1236I CGCG 2 cut(s) 581, 730
BsiHKCI CYCGRG 1 cut(s) 1178
BsiSI CCGG 1 cut(s) 450
BslFI GGGAC 2 cut(s) 722, 734
BslI CCNNNNNNNGG 1 cut(s) 752
BsmAI GTCTC 1 cut(s) 259
BsmFI GGGAC 2 cut(s) 722, 734
BsmI GAATGC 2 cut(s) 113, 115
BsoBI CYCGRG 1 cut(s) 1178
Bsp1407I TGTACA 1 cut(s) 864
Bsp143I GATC 3 cut(s) 443, 811, 855
Bsp1720I GCTNAGC 1 cut(s) 409
Bsp19I CCATGG 2 cut(s) 988, 998
BspACI CCGC 6 cut(s) 123, 447, 495, 995, 1115, 1167
BspCNI CTCAG 3 cut(s) 255, 282, 516
BspFNI CGCG 2 cut(s) 581, 730
BspHI TCATGA 1 cut(s) 1077
BspLI GGNNCC 4 cut(s) 312, 445, 493, 956
BspMAI CTGCAG 2 cut(s) 677, 904
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 2 cut(s) 438, 451
BsrDI GCAATG 1 cut(s) 945
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 6 cut(s) 104, 257, 537, 988, 998, 1147
BssHII GCGCGC 1 cut(s) 579
BssMI GATC 3 cut(s) 443, 811, 855
BssT1I CCWWGG 6 cut(s) 104, 257, 537, 988, 998, 1147
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 6 cut(s) 385, 658, 775, 862, 1087, 1099
BstAUI TGTACA 1 cut(s) 864
BstDEI CTNAG 4 cut(s) 263, 269, 409, 524
BstDSI CCRYGG 2 cut(s) 988, 998
BstFNI CGCG 2 cut(s) 581, 730
BstHHI GCGC 4 cut(s) 581, 583, 704, 730
BstKTI GATC 3 cut(s) 446, 814, 858
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 3 cut(s) 443, 811, 855
BstMWI GCNNNNNNNGC 6 cut(s) 485, 494, 938, 947, 1112, 1164
BstNI CCWGG 1 cut(s) 795
BstNSI RCATGY 2 cut(s) 164, 475
BstSCI CCNGG 1 cut(s) 793
BstSFI CTRYAG 2 cut(s) 673, 900
BstUI CGCG 2 cut(s) 581, 730
BstV1I GCAGC 1 cut(s) 608
BstV2I GAAGAC 1 cut(s) 166
BstX2I RGATCY 2 cut(s) 443, 811
BstXI CCANNNNNNTGG 2 cut(s) 717, 1135
BstYI RGATCY 2 cut(s) 443, 811
BsuI GTATCC 1 cut(s) 852
BtgI CCRYGG 2 cut(s) 988, 998
BtgZI GCGATG 1 cut(s) 557
BtrI CACGTC 1 cut(s) 724
BtsI GCAGTG 3 cut(s) 358, 670, 975
BtsIMutI CAGTG 4 cut(s) 358, 670, 780, 975
BveI ACCTGC 1 cut(s) 359
CciI TCATGA 1 cut(s) 1077
CfoI GCGC 4 cut(s) 581, 583, 704, 730
Cfr13I GGNCC 1 cut(s) 992
Csp6I GTAC 3 cut(s) 203, 589, 865
CviAII CATG 9 cut(s) 161, 190, 228, 472, 661, 989, 999, 1078, 1135
CviQI GTAC 3 cut(s) 203, 589, 865
DdeI CTNAG 4 cut(s) 263, 269, 409, 524
DpnI GATC 3 cut(s) 445, 813, 857
DpnII GATC 3 cut(s) 443, 811, 855
EciI GGCGGA 1 cut(s) 436
Eco130I CCWWGG 6 cut(s) 104, 257, 537, 988, 998, 1147
Eco47I GGWCC 1 cut(s) 992
Eco57I CTGAAG 2 cut(s) 486, 1131
Eco88I CYCGRG 1 cut(s) 1178
EcoRII CCWGG 1 cut(s) 793
EcoT14I CCWWGG 6 cut(s) 104, 257, 537, 988, 998, 1147
EcoT22I ATGCAT 1 cut(s) 115
ErhI CCWWGG 6 cut(s) 104, 257, 537, 988, 998, 1147
FaeI CATG 9 cut(s) 164, 193, 231, 475, 664, 992, 1002, 1081, 1138
FalI AAGNNNNNCTT 4 cut(s) 34, 66, 919, 951
FaqI GGGAC 2 cut(s) 722, 734
FatI CATG 9 cut(s) 160, 189, 227, 471, 660, 988, 998, 1077, 1134
FbaI TGATCA 1 cut(s) 855
Fnu4HI GCNGC 2 cut(s) 622, 1168
Fsp4HI GCNGC 2 cut(s) 622, 1168
FspBI CTAG 1 cut(s) 258
FspI TGCGCA 1 cut(s) 703
GlaI GCGC 4 cut(s) 580, 582, 703, 729
GluI GCNGC 2 cut(s) 622, 1168
GsaI CCCAGC 2 cut(s) 40, 86
HapII CCGG 1 cut(s) 450
HhaI GCGC 4 cut(s) 581, 583, 704, 730
Hin1II CATG 9 cut(s) 164, 193, 231, 475, 664, 992, 1002, 1081, 1138
Hin6I GCGC 4 cut(s) 579, 581, 702, 728
HinP1I GCGC 4 cut(s) 579, 581, 702, 728
HincII GTYRAC 1 cut(s) 654
HindII GTYRAC 1 cut(s) 654
HindIII AAGCTT 3 cut(s) 433, 455, 824
HinfI GANTC 4 cut(s) 253, 267, 778, 818
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 654, 865
Hpy188I TCNGA 4 cut(s) 462, 1065, 1092, 1111
Hpy188III TCNNGA 6 cut(s) 166, 686, 815, 833, 1078, 1189
Hpy8I GTNNAC 2 cut(s) 654, 865
HpyAV CCTTC 1 cut(s) 163
HpyCH4III ACNGT 6 cut(s) 385, 658, 775, 862, 1087, 1099
HpyCH4IV ACGT 1 cut(s) 723
HpyF10VI GCNNNNNNNGC 6 cut(s) 485, 494, 938, 947, 1112, 1164
HpyF3I CTNAG 4 cut(s) 263, 269, 409, 524
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 9 cut(s) 164, 193, 231, 475, 664, 992, 1002, 1081, 1138
HspAI GCGC 4 cut(s) 579, 581, 702, 728
Ksp22I TGATCA 1 cut(s) 855
Kzo9I GATC 3 cut(s) 443, 811, 855
LmnI GCTCC 4 cut(s) 86, 316, 960, 1117
Lsp1109I GCAGC 1 cut(s) 608
LweI GCATC 4 cut(s) 37, 151, 398, 534
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 379, 763, 775
MalI GATC 3 cut(s) 445, 813, 857
MboI GATC 3 cut(s) 443, 811, 855
MboII GAAGA 7 cut(s) 166, 189, 259, 492, 821, 902, 964
MflI RGATCY 2 cut(s) 443, 811
MluCI AATT 5 cut(s) 94, 315, 513, 713, 1014
MlyI GAGTC 3 cut(s) 261, 262, 772
MnlI CCTC 4 cut(s) 422, 519, 623, 995
Mph1103I ATGCAT 1 cut(s) 115
MroXI GAANNNNTTC 3 cut(s) 113, 173, 496
MseI TTAA 2 cut(s) 302, 924
MslI CAYNNNNRTG 3 cut(s) 1037, 1133, 1139
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 795
Mva1269I GAATGC 2 cut(s) 113, 115
MvaI CCWGG 1 cut(s) 795
MvnI CGCG 2 cut(s) 581, 730
MwoI GCNNNNNNNGC 6 cut(s) 485, 494, 938, 947, 1112, 1164
NcoI CCATGG 2 cut(s) 988, 998
NdeII GATC 3 cut(s) 443, 811, 855
NlaIII CATG 9 cut(s) 164, 193, 231, 475, 664, 992, 1002, 1081, 1138
NlaIV GGNNCC 4 cut(s) 312, 445, 493, 956
NmuCI GTSAC 2 cut(s) 379, 775
NsbI TGCGCA 1 cut(s) 703
NsiI ATGCAT 1 cut(s) 115
NspI RCATGY 2 cut(s) 164, 475
PagI TCATGA 1 cut(s) 1077
PauI GCGCGC 1 cut(s) 579
PctI GAATGC 2 cut(s) 113, 115
PdmI GAANNNNTTC 3 cut(s) 113, 173, 496
PfeI GAWTC 1 cut(s) 818
PflFI GACNNNGTC 1 cut(s) 781
PflMI CCANNNNNTGG 1 cut(s) 752
PfoI TCCNGGA 1 cut(s) 793
PkrI GCNGC 2 cut(s) 623, 1169
PleI GAGTC 3 cut(s) 261, 261, 772
PpsI GAGTC 3 cut(s) 261, 261, 772
Psp6I CCWGG 1 cut(s) 793
PspFI CCCAGC 2 cut(s) 36, 82
PspGI CCWGG 1 cut(s) 793
PspN4I GGNNCC 4 cut(s) 312, 445, 493, 956
PspPI GGNCC 1 cut(s) 992
PstI CTGCAG 2 cut(s) 677, 904
PsuI RGATCY 2 cut(s) 443, 811
PsyI GACNNNGTC 1 cut(s) 781
PteI GCGCGC 1 cut(s) 579
RsaI GTAC 3 cut(s) 204, 590, 866
RsaNI GTAC 3 cut(s) 203, 589, 865
RseI CAYNNNNRTG 3 cut(s) 1037, 1133, 1139
SaqAI TTAA 2 cut(s) 302, 924
SatI GCNGC 2 cut(s) 622, 1168
Sau3AI GATC 3 cut(s) 443, 811, 855
Sau96I GGNCC 1 cut(s) 992
SchI GAGTC 3 cut(s) 261, 262, 772
ScrFI CCNGG 1 cut(s) 795
SfaNI GCATC 4 cut(s) 37, 151, 398, 534
SfcI CTRYAG 2 cut(s) 673, 900
SinI GGWCC 1 cut(s) 992
SmiMI CAYNNNNRTG 3 cut(s) 1037, 1133, 1139
SmlI CTYRAG 1 cut(s) 684
SmoI CTYRAG 1 cut(s) 684
Sse9I AATT 5 cut(s) 94, 315, 513, 713, 1014
SsiI CCGC 6 cut(s) 123, 447, 495, 995, 1115, 1167
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 793
StyI CCWWGG 6 cut(s) 104, 257, 537, 988, 998, 1147
TaaI ACNGT 6 cut(s) 385, 658, 775, 862, 1087, 1099
TaiI ACGT 1 cut(s) 726
TaqI TCGA 4 cut(s) 165, 785, 816, 832
TasI AATT 5 cut(s) 94, 315, 513, 713, 1014
TatI WGTACW 2 cut(s) 588, 864
TauI GCSGC 1 cut(s) 1170
TfiI GAWTC 1 cut(s) 818
Tru1I TTAA 2 cut(s) 302, 924
Tru9I TTAA 2 cut(s) 302, 924
TscAI CASTG 4 cut(s) 358, 677, 780, 982
TseFI GTSAC 2 cut(s) 379, 775
TseI GCWGC 1 cut(s) 621
Tsp45I GTSAC 2 cut(s) 379, 775
TspDTI ATGAA 3 cut(s) 62, 106, 583
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 4 cut(s) 358, 677, 780, 982
Tth111I GACNNNGTC 1 cut(s) 781
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 1 cut(s) 992
XapI RAATTY 2 cut(s) 713, 1014
XceI RCATGY 2 cut(s) 164, 475
XcmI CCANNNNNNNNNTGG 2 cut(s) 321, 1135
XmaJI CCTAGG 1 cut(s) 257
XmnI GAANNNNTTC 3 cut(s) 113, 173, 496
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.