MD02G1043600.v1.1

protein ABIL5 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013584
Physical Location & Seq
Forward (+)
3561844 .. 3564411
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1043600.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGAGGAGGAGATGCAGAGCAACTCCAAATCCCCCTCAGGGAGAGGGCAAGAATCAGAGGCAGAGTCTAAGGACATTGTGAGCTTTGATAAGTCTCTTCAGGAACTAAGAGACCTGCGCTCACAACTTCACTATGCTGCTGACTACTGTGAATCAACCTTCCTAAAGTCCAAAGAAAAGAAAGTTGTGATGGAAAACACCAAAGAGTACATATGCAGAGCTGTGGTGACTGTTGTCGATCATCTTGGATGCGTTTCTGCCAACCTCAACGGCCTCATTTCTGAAACTAACACATTTTCGGAGGCTGAGATTCGAATTGACTGCCTCAAACAAAGGATGTTCCTATGTGAACAATACACCCACAAATTTTCCCTGCCTAGAGTCCGATGGAAGGACATTATCCCGCAGCATAAAGCAAGGTTTTTATCTGCAGCTACCAAAGAAGCTGAGAAACCCAATGACGATTTGAGGGCTATTCCAGGAAATCCAGCCTCTCGTAAAACCATTGACAAGCAAGAACTTGGCAAAGATGTAGCAATGCCACTTTCCTTGTACACCTCGTCTCACAAACCGTCATCGTCCAAGGGCGAAAACAATTCAGTTTTAGTGCCAGTCCGTGATGGCCTCTCAATCCTGTCTAGAGGTCCAAACCCTACATTTCATTTCCAGGAATCTCGGAAGATTACTGGCCGCCTCAAGAAATCTGGGCACAGTAATGACATCTTGGCACTCGTTAGACGAGCAAAAAGAATGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

28.44

Weight (kDa)

9.21

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 123
AciI CCGC 2 cut(s) 404, 691
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 2 cut(s) 209, 554
AfiI CCNNNNNNNGG 2 cut(s) 39, 391
AjnI CCWGG 2 cut(s) 478, 666
AluBI AGCT 4 cut(s) 84, 221, 434, 446
AluI AGCT 4 cut(s) 84, 221, 434, 446
Alw26I GTCTC 3 cut(s) 99, 105, 567
AoxI GGCC 3 cut(s) 271, 622, 688
ApeKI GCWGC 3 cut(s) 137, 406, 431
ApoI RAATTY 1 cut(s) 365
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 644
AsuHPI GGTGA 1 cut(s) 238
AsuII TTCGAA 1 cut(s) 313
AvaII GGWCC 1 cut(s) 644
AxyI CCTNAGG 1 cut(s) 37
BaeGI GKGCMC 1 cut(s) 711
BbvI GCAGC 3 cut(s) 124, 418, 443
BccI CCATC 3 cut(s) 184, 381, 614
BceAI ACGGC 1 cut(s) 286
BcgI CGANNNNNNTGC 2 cut(s) 303, 337
BciT130I CCWGG 2 cut(s) 480, 668
BcoDI GTCTC 3 cut(s) 99, 105, 567
BfaI CTAG 2 cut(s) 378, 639
BfmI CTRYAG 1 cut(s) 429
BfuAI ACCTGC 1 cut(s) 123
BisI GCNGC 4 cut(s) 138, 407, 432, 691
BlsI GCNGC 4 cut(s) 139, 408, 433, 692
Bme1390I CCNGG 2 cut(s) 480, 668
Bme18I GGWCC 1 cut(s) 644
BmgT120I GGNCC 1 cut(s) 644
BmrFI CCNGG 2 cut(s) 480, 668
BmsI GCATC 2 cut(s) 3, 239
BoxI GACNNNNGTC 1 cut(s) 233
Bpu14I TTCGAA 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 680
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 582
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 391
Bse1I ACTGG 2 cut(s) 611, 691
Bse21I CCTNAGG 1 cut(s) 37
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 2 cut(s) 480, 668
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 2 cut(s) 254, 342
BseLI CCNNNNNNNGG 2 cut(s) 39, 391
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 3 cut(s) 51, 297, 438
BseNI ACTGG 2 cut(s) 611, 691
BseRI GAGGAG 2 cut(s) 20, 23
BseSI GKGCMC 1 cut(s) 711
BseXI GCAGC 3 cut(s) 124, 418, 443
BshFI GGCC 3 cut(s) 273, 624, 690
BslI CCNNNNNNNGG 2 cut(s) 39, 391
BsmAI GTCTC 3 cut(s) 99, 105, 567
BsmBI CGTCTC 1 cut(s) 567
BsnI GGCC 3 cut(s) 273, 624, 690
Bso31I GGTCTC 1 cut(s) 105
Bsp119I TTCGAA 1 cut(s) 313
Bsp1286I GDGCHC 1 cut(s) 711
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 2 cut(s) 404, 691
BspANI GGCC 3 cut(s) 273, 624, 690
BspCNI CTCAG 3 cut(s) 50, 298, 439
BspMAI CTGCAG 1 cut(s) 433
BspMI ACCTGC 1 cut(s) 123
BspT104I TTCGAA 1 cut(s) 313
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 543
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 2 cut(s) 611, 691
BssECI CCNNGG 1 cut(s) 582
BssMI GATC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 2 cut(s) 480, 668
Bst4CI ACNGT 4 cut(s) 149, 232, 573, 713
Bst6I CTCTTC 1 cut(s) 102
BstAUI TGTACA 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 313
BstDEI CTNAG 5 cut(s) 37, 69, 107, 306, 447
BstF5I GGATG 2 cut(s) 254, 342
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 3 cut(s) 99, 105, 567
BstMBI GATC 1 cut(s) 238
BstNI CCWGG 2 cut(s) 480, 668
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 2 cut(s) 478, 666
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 711
BstV1I GCAGC 3 cut(s) 124, 418, 443
Bsu36I CCTNAGG 1 cut(s) 37
BsuRI GGCC 3 cut(s) 273, 624, 690
BtsCI GGATG 2 cut(s) 254, 342
BveI ACCTGC 1 cut(s) 123
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 644
Csp6I GTAC 2 cut(s) 208, 553
CspCI CAANNNNNGTGG 2 cut(s) 531, 566
CviQI GTAC 2 cut(s) 208, 553
DdeI CTNAG 5 cut(s) 37, 69, 107, 306, 447
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 688
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 582
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 1 cut(s) 83
Eco81I CCTNAGG 1 cut(s) 37
EcoRII CCWGG 2 cut(s) 478, 666
EcoT14I CCWWGG 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 582
Esp3I CGTCTC 1 cut(s) 567
FaiI YATR 6 cut(s) 135, 212, 214, 346, 411, 757
FauI CCCGC 1 cut(s) 411
FauNDI CATATG 1 cut(s) 212
Fnu4HI GCNGC 4 cut(s) 138, 407, 432, 691
FokI GGATG 2 cut(s) 261, 349
Fsp4HI GCNGC 4 cut(s) 138, 407, 432, 691
FspBI CTAG 2 cut(s) 378, 639
GlaI GCGC 1 cut(s) 119
GluI GCNGC 4 cut(s) 138, 407, 432, 691
HaeIII GGCC 3 cut(s) 273, 624, 690
HhaI GCGC 1 cut(s) 120
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 6 cut(s) 53, 65, 152, 310, 381, 671
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 350, 555
Hpy188I TCNGA 5 cut(s) 58, 283, 301, 386, 678
Hpy188III TCNNGA 3 cut(s) 101, 639, 697
Hpy8I GTNNAC 2 cut(s) 350, 555
HpyAV CCTTC 2 cut(s) 169, 385
HpyCH4III ACNGT 4 cut(s) 149, 232, 573, 713
HpyCH4V TGCA 3 cut(s) 16, 216, 431
HpyF3I CTNAG 5 cut(s) 37, 69, 107, 306, 447
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 1 cut(s) 238
Lsp1109I GCAGC 3 cut(s) 124, 418, 443
LweI GCATC 2 cut(s) 3, 239
MaeI CTAG 2 cut(s) 378, 639
MaeIII GTNAC 1 cut(s) 226
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 2 cut(s) 89, 691
MhlI GDGCHC 1 cut(s) 711
MluCI AATT 3 cut(s) 315, 365, 595
MlyI GAGTC 2 cut(s) 74, 390
MslI CAYNNNNRTG 1 cut(s) 714
MspR9I CCNGG 2 cut(s) 480, 668
MvaI CCWGG 2 cut(s) 480, 668
NdeI CATATG 1 cut(s) 212
NdeII GATC 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 226
NspV TTCGAA 1 cut(s) 313
PfeI GAWTC 4 cut(s) 53, 152, 310, 671
PfoI TCCNGGA 2 cut(s) 478, 666
PkrI GCNGC 4 cut(s) 139, 408, 433, 692
PleI GAGTC 2 cut(s) 73, 389
PpsI GAGTC 2 cut(s) 73, 389
PshAI GACNNNNGTC 1 cut(s) 233
Psp6I CCWGG 2 cut(s) 478, 666
PspGI CCWGG 2 cut(s) 478, 666
PspPI GGNCC 1 cut(s) 644
PstI CTGCAG 1 cut(s) 433
RsaI GTAC 2 cut(s) 209, 554
RsaNI GTAC 2 cut(s) 208, 553
RseI CAYNNNNRTG 1 cut(s) 714
SatI GCNGC 4 cut(s) 138, 407, 432, 691
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 2 cut(s) 74, 390
ScrFI CCNGG 2 cut(s) 480, 668
SduI GDGCHC 1 cut(s) 711
SfaNI GCATC 2 cut(s) 3, 239
SfcI CTRYAG 1 cut(s) 429
SfuI TTCGAA 1 cut(s) 313
SinI GGWCC 1 cut(s) 644
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
Sse9I AATT 3 cut(s) 315, 365, 595
SsiI CCGC 2 cut(s) 404, 691
SspMI CTAG 2 cut(s) 378, 639
StyD4I CCNGG 2 cut(s) 478, 666
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 4 cut(s) 149, 232, 573, 713
TaqI TCGA 2 cut(s) 237, 313
TasI AATT 3 cut(s) 315, 365, 595
TatI WGTACW 2 cut(s) 207, 552
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 4 cut(s) 53, 152, 310, 671
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 3 cut(s) 137, 406, 431
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 650
TspGWI ACGGA 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 1 cut(s) 365
XbaI TCTAGA 1 cut(s) 638
XspI CTAG 2 cut(s) 378, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.