MD15G1182100.v1.1

protein ABIL5 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013584
Physical Location & Seq
Forward (+)
14444160 .. 14446439
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1182100.v1.1.491

Sequence Viewer

Length: 834 bp
ATGGATGAGAAGATGCAGAACAACTCTAAACCCTCCTCGGGGCAAGATGCAGAAGCAGAGTCTAAGGACGTTCCAAACATGGAGGAGAAGATGCAGAACATCTCTAAACCCTCCTCAGGGCAAGATGTAGAAGCAAAGTCTAAGGACATTGGAAGCTTTCATAACTCTCTTCAGGAACTGAGAGACCTGCGCTCTCAACTTCACTATGCTGCTGACTACTGTGAATCAACCTTCTTGAACACCAAAGAAAAGAAAGTAGTGATGGAAAACACCAAAGAGTATATCTGCAGAGCTGTGGTGACTGTTGTTGATCATCTCGGATTCGTTTCCGCCAACATCAACGGCATCGTTTCCGAAGCTAACGCATTTTCAGAGGCTGAGATTCGAATTGATTGCCTCAAACAAGTTAGGATGTTCTTATGTGAACAATACAGCCACAAGTTTGCCCTGCCTAGAGTCCGATGGAGGGAGATTATCCCGCGGCATAATGCACGTTTTTTATCTGCACCTACCAGAGAAGCTGAGAAACCCAATGAAGATTCGAGGGATATTCCTAGAAACGCAGCCTCTCATAAAACCATTGACAAGCAAGAATCTGACCGAGATGTAGCAATGCCGCTTTTCTTGTACACACCGTCTCACAAACCGTCTTTGTCCAAGGGCGAAAACAATTCAGCTTTAGCTCCGGTTCGTGATGGCCTCTCAATCCTGTCTAGAGGCCCAAATCTTACCTTTCATTTCCAGGAAGCTCGGAAGAATAATGGACGCCTCAAGAAATCTGGGCACGGTAATGACATCTTGTCGCTCATCAGACGAGCGAAAAGAACAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

278

Amino Acids

31.35

Weight (kDa)

8.94

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 195
AccII CGCG 1 cut(s) 481
AciI CCGC 4 cut(s) 330, 479, 481, 617
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 766
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 5 cut(s) 38, 39, 116, 117, 466
AgsI TTSAA 1 cut(s) 238
AhdI GACNNNNNGTC 1 cut(s) 799
AjnI CCWGG 1 cut(s) 741
AluBI AGCT 7 cut(s) 156, 293, 359, 521, 677, 683, 749
AluI AGCT 7 cut(s) 156, 293, 359, 521, 677, 683, 749
Alw26I GTCTC 2 cut(s) 177, 642
AlwNI CAGNNNCTG 2 cut(s) 178, 377
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 2 cut(s) 697, 718
ApeKI GCWGC 2 cut(s) 209, 563
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 719
AsuHPI GGTGA 1 cut(s) 310
AsuII TTCGAA 1 cut(s) 385
AvaI CYCGRG 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 786
BbvI GCAGC 2 cut(s) 196, 575
BccI CCATC 3 cut(s) 256, 456, 689
BceAI ACGGC 1 cut(s) 358
BcgI CGANNNNNNTGC 2 cut(s) 375, 409
BciT130I CCWGG 1 cut(s) 743
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 2 cut(s) 177, 642
BfaI CTAG 3 cut(s) 453, 555, 714
BfmI CTRYAG 1 cut(s) 286
BfuAI ACCTGC 1 cut(s) 195
BisI GCNGC 4 cut(s) 210, 482, 564, 617
BlsI GCNGC 4 cut(s) 211, 483, 565, 618
Bme1390I CCNGG 1 cut(s) 743
BmeRI GACNNNNNGTC 1 cut(s) 799
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 719
BmrFI CCNGG 1 cut(s) 743
BmsI GCATC 4 cut(s) 3, 37, 81, 354
Bpu14I TTCGAA 1 cut(s) 385
BpuEI CTTGAG 1 cut(s) 755
BsaHI GRCGYC 1 cut(s) 766
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 36, 479, 657
BsaWI WCCGGW 1 cut(s) 685
Bsc4I CCNNNNNNNGG 5 cut(s) 38, 39, 116, 117, 466
Bse21I CCTNAGG 1 cut(s) 115
Bse3DI GCAATG 1 cut(s) 618
BseBI CCWGG 1 cut(s) 743
BseDI CCNNGG 3 cut(s) 36, 479, 657
BseGI GGATG 2 cut(s) 10, 417
BseLI CCNNNNNNNGG 5 cut(s) 38, 39, 116, 117, 466
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 4 cut(s) 129, 170, 369, 513
BseRI GAGGAG 3 cut(s) 25, 98, 103
BseSI GKGCMC 1 cut(s) 786
BseXI GCAGC 2 cut(s) 196, 575
BsgI GTGCAG 1 cut(s) 489
Bsh1236I CGCG 1 cut(s) 481
BshFI GGCC 2 cut(s) 699, 720
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 686
BslI CCNNNNNNNGG 5 cut(s) 38, 39, 116, 117, 466
BsmAI GTCTC 2 cut(s) 177, 642
BsmBI CGTCTC 1 cut(s) 642
BsnI GGCC 2 cut(s) 699, 720
Bso31I GGTCTC 1 cut(s) 177
BsoBI CYCGRG 1 cut(s) 37
Bsp119I TTCGAA 1 cut(s) 385
Bsp1286I GDGCHC 1 cut(s) 786
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 1 cut(s) 310
BspACI CCGC 4 cut(s) 330, 479, 481, 617
BspANI GGCC 2 cut(s) 699, 720
BspCNI CTCAG 4 cut(s) 128, 171, 370, 514
BspFNI CGCG 1 cut(s) 481
BspMAI CTGCAG 1 cut(s) 290
BspMI ACCTGC 1 cut(s) 195
BspT104I TTCGAA 1 cut(s) 385
BspTNI GGTCTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 618
BsrGI TGTACA 1 cut(s) 627
BssECI CCNNGG 3 cut(s) 36, 479, 657
BssMI GATC 1 cut(s) 310
BssNI GRCGYC 1 cut(s) 766
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 743
Bst4CI ACNGT 6 cut(s) 221, 304, 636, 648, 788, 829
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 766
BstAUI TGTACA 1 cut(s) 627
BstBI TTCGAA 1 cut(s) 385
BstDEI CTNAG 6 cut(s) 63, 115, 141, 179, 378, 522
BstDSI CCRYGG 1 cut(s) 479
BstF5I GGATG 2 cut(s) 10, 417
BstFNI CGCG 1 cut(s) 481
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 2 cut(s) 177, 642
BstMBI GATC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 743
BstSCI CCNGG 1 cut(s) 741
BstSFI CTRYAG 1 cut(s) 286
BstSLI GKGCMC 1 cut(s) 786
BstUI CGCG 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 196, 575
Bsu36I CCTNAGG 1 cut(s) 115
BsuRI GGCC 2 cut(s) 699, 720
BtgI CCRYGG 1 cut(s) 479
BtsCI GGATG 2 cut(s) 10, 417
BveI ACCTGC 1 cut(s) 195
CaiI CAGNNNCTG 2 cut(s) 178, 377
CfoI GCGC 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 719
Cfr42I CCGCGG 1 cut(s) 482
CseI GACGC 1 cut(s) 774
Csp6I GTAC 1 cut(s) 628
CviAII CATG 1 cut(s) 79
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 6 cut(s) 63, 115, 141, 179, 378, 522
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
DriI GACNNNNNGTC 1 cut(s) 799
Eam1104I CTCTTC 1 cut(s) 174
Eam1105I GACNNNNNGTC 1 cut(s) 799
EarI CTCTTC 1 cut(s) 174
EciI GGCGGA 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 155
Eco81I CCTNAGG 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 741
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 1 cut(s) 82
FaiI YATR 8 cut(s) 80, 162, 207, 282, 421, 486, 573, 832
FatI CATG 1 cut(s) 78
FauI CCCGC 1 cut(s) 486
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 4 cut(s) 210, 482, 564, 617
FokI GGATG 2 cut(s) 17, 424
Fsp4HI GCNGC 4 cut(s) 210, 482, 564, 617
FspBI CTAG 3 cut(s) 453, 555, 714
GlaI GCGC 1 cut(s) 191
GluI GCNGC 4 cut(s) 210, 482, 564, 617
HaeIII GGCC 2 cut(s) 699, 720
HapII CCGG 1 cut(s) 686
HgaI GACGC 1 cut(s) 774
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 766
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 7 cut(s) 59, 224, 321, 382, 456, 539, 593
HpaII CCGG 1 cut(s) 686
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 2 cut(s) 425, 630
Hpy188I TCNGA 7 cut(s) 320, 355, 373, 461, 598, 753, 812
Hpy188III TCNNGA 5 cut(s) 173, 235, 692, 714, 772
Hpy8I GTNNAC 2 cut(s) 425, 630
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 6 cut(s) 221, 304, 636, 648, 788, 829
HpyCH4IV ACGT 2 cut(s) 69, 493
HpyCH4V TGCA 6 cut(s) 16, 50, 94, 288, 491, 506
HpyF3I CTNAG 6 cut(s) 63, 115, 141, 179, 378, 522
HpySE526I ACGT 2 cut(s) 69, 493
Hsp92I GRCGYC 1 cut(s) 766
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 310
KspI CCGCGG 1 cut(s) 482
Kzo9I GATC 1 cut(s) 310
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 2 cut(s) 196, 575
LweI GCATC 4 cut(s) 3, 37, 81, 354
MaeI CTAG 3 cut(s) 453, 555, 714
MaeII ACGT 2 cut(s) 69, 493
MaeIII GTNAC 1 cut(s) 298
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 5 cut(s) 22, 100, 161, 548, 766
MhlI GDGCHC 1 cut(s) 786
MluCI AATT 2 cut(s) 387, 670
MlyI GAGTC 2 cut(s) 68, 465
MslI CAYNNNNRTG 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 481
MspI CCGG 1 cut(s) 686
MspR9I CCNGG 1 cut(s) 743
MvaI CCWGG 1 cut(s) 743
MvnI CGCG 1 cut(s) 481
NdeII GATC 1 cut(s) 310
NlaIII CATG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 385
PfeI GAWTC 5 cut(s) 224, 321, 382, 539, 593
PfoI TCCNGGA 1 cut(s) 741
PkrI GCNGC 4 cut(s) 211, 483, 565, 618
PleI GAGTC 2 cut(s) 67, 464
PpsI GAGTC 2 cut(s) 67, 464
Psp6I CCWGG 1 cut(s) 741
PspGI CCWGG 1 cut(s) 741
PspPI GGNCC 1 cut(s) 719
PstI CTGCAG 1 cut(s) 290
PstNI CAGNNNCTG 2 cut(s) 178, 377
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
RseI CAYNNNNRTG 1 cut(s) 789
SacII CCGCGG 1 cut(s) 482
SatI GCNGC 4 cut(s) 210, 482, 564, 617
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 1 cut(s) 719
SchI GAGTC 2 cut(s) 68, 465
ScrFI CCNGG 1 cut(s) 743
SduI GDGCHC 1 cut(s) 786
SfaNI GCATC 4 cut(s) 3, 37, 81, 354
SfcI CTRYAG 1 cut(s) 286
Sfr303I CCGCGG 1 cut(s) 482
SfuI TTCGAA 1 cut(s) 385
SgrBI CCGCGG 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 2 cut(s) 387, 670
SsiI CCGC 4 cut(s) 330, 479, 481, 617
SspMI CTAG 3 cut(s) 453, 555, 714
StyD4I CCNGG 1 cut(s) 741
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 6 cut(s) 221, 304, 636, 648, 788, 829
TaiI ACGT 2 cut(s) 72, 496
TaqI TCGA 2 cut(s) 385, 542
TaqII GACCGA 1 cut(s) 615
TasI AATT 2 cut(s) 387, 670
TatI WGTACW 1 cut(s) 627
TauI GCSGC 2 cut(s) 484, 619
TfiI GAWTC 5 cut(s) 224, 321, 382, 539, 593
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 2 cut(s) 209, 563
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 149, 549, 725
XbaI TCTAGA 1 cut(s) 713
XspI CTAG 3 cut(s) 453, 555, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.