MD02G1048400.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013103
Physical Location & Seq
Forward (+)
3815527 .. 3817488
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1048400.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGCCTCCTCCAAGAGCGAGTACGCATTCTACTCGCCGATCCCGTCCGGCCTCAACAACCCACAGCCTCAGCAAAACGTCGTCGTTCTAACTCATTACCGTCCCACTCCCCACTACTGCTGCGGACGCTCTCTCCGCCTCTGCATCTCAATCACCGCCACCATCCTTCTCCTCTCTGCCGCCGTCTTCTTCCTCTACCCCTCCGACCCCACCCTCCACCTCGCCCGAATCAAACTCAACCACGTCCGAGTCAACGCGTCCCCCAAACCCACTCTCGACCTATCCTTCTCTCTCACCATCAGAGTTCGAAACCGCGACTTCTTCTCCCTGAATTACGACTCGCTCAAAGTCACGGTGGGGTACCGCGCGAGCGAGCTAGGGTTTGTGATCTCCGACGGAGGGCACGTCAGGGCCAGAGCGTCGTCGTACGTGAACGCCACGCTCGTGGTCGACGGGCTTGAGGTCCTCCACGACGTGTTTTACTTGCTTGAGGATTTGGCGAAAGGGGTAATTCCCTTTGATACCGATACGGCGGTGGATGGAACTTTGGGGGTTTTGTTCTTCAAGATTCCGATCAAGGCAAGAGCATCGTGTGAAGTATATGTAGATACAAACAATCAGACGGTTGTTCGTGAAGACTGCTACTCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

218

Amino Acids

24.08

Weight (kDa)

7.0

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 194 9.8e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13270
fragaria_vesca FvH4_1g04970
malus_domestica MD02G1048400.v1.1 MD15G1186800.v1.1
prunus_persica Prupe.7G229800_v2.0.a1
pyrus_communis pycom02g04060 pycom15g16730
rosa_chinensis RchiOBHm_Chr2g0090601
rosa_laevigata RLG00000016123
rosa_multiflora Rmu_sc0020367.1_g000001 Rmu_sc0040185.1_g000001
rosa_roxburghii Rroxscaffold_2G00151000
rosa_rugosa Rorug02G0009300
rosa_samantha Rh2AG054600 Rh2BG053800 Rh2CG056000 Rh2DG054900
rosa_wichuraiana Rw2G004730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 360
AccB1I GGYRCC 1 cut(s) 360
AccI GTMKAC 1 cut(s) 450
AccII CGCG 4 cut(s) 257, 315, 366, 368
AciI CCGC 7 cut(s) 123, 136, 156, 180, 313, 364, 533
AclWI GGATC 1 cut(s) 34
AdeI CACNNNGTG 1 cut(s) 475
AfaI GTAC 3 cut(s) 23, 362, 428
AfiI CCNNNNNNNGG 1 cut(s) 399
AflIII ACRYGT 2 cut(s) 255, 474
AgsI TTSAA 1 cut(s) 565
AjiI CACGTC 3 cut(s) 244, 406, 475
AleI CACNNNNGTG 1 cut(s) 443
AluBI AGCT 1 cut(s) 376
AluI AGCT 1 cut(s) 376
AlwI GGATC 1 cut(s) 34
AoxI GGCC 3 cut(s) 3, 49, 411
ApeKI GCWGC 1 cut(s) 120
Asp718I GGTACC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 411, 463
AsuHPI GGTGA 2 cut(s) 145, 286
AsuII TTCGAA 1 cut(s) 307
AvaII GGWCC 1 cut(s) 463
BaeGI GKGCMC 1 cut(s) 405
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BanI GGYRCC 1 cut(s) 360
BauI CACGAG 1 cut(s) 443
BbsI GAAGAC 2 cut(s) 178, 642
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 107
BccI CCATC 3 cut(s) 170, 305, 533
BceAI ACGGC 2 cut(s) 167, 546
BcgI CGANNNNNNTGC 4 cut(s) 15, 49, 570, 604
BfaI CTAG 1 cut(s) 377
BisI GCNGC 2 cut(s) 121, 180
BlsI GCNGC 2 cut(s) 122, 181
Bme18I GGWCC 1 cut(s) 463
BmgBI CACGTC 3 cut(s) 244, 406, 475
BmgT120I GGNCC 2 cut(s) 411, 463
BmiI GGNNCC 1 cut(s) 362
BmsI GCATC 2 cut(s) 153, 596
BpiI GAAGAC 2 cut(s) 178, 642
Bpu10I CCTNAGC 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 307
BpuEI CTTGAG 2 cut(s) 479, 509
BsaAI YACGTR 1 cut(s) 430
BsaBI GATNNNNATC 1 cut(s) 572
BsaXI ACNNNNNCTCC 6 cut(s) 117, 147, 308, 338, 390, 420
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse8I GATNNNNATC 1 cut(s) 572
BseGI GGATG 2 cut(s) 162, 544
BseJI GATNNNNATC 1 cut(s) 572
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 83, 639
BseRI GAGGAG 1 cut(s) 161
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 4 cut(s) 257, 315, 366, 368
BshFI GGCC 3 cut(s) 5, 51, 413
BshNI GGYRCC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 48
BsiWI CGTACG 1 cut(s) 426
BslFI GGGAC 2 cut(s) 87, 244
BslI CCNNNNNNNGG 1 cut(s) 399
BsmFI GGGAC 2 cut(s) 87, 244
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 3 cut(s) 5, 51, 413
Bsp119I TTCGAA 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 3 cut(s) 39, 387, 573
BspACI CCGC 7 cut(s) 123, 136, 156, 180, 313, 364, 533
BspANI GGCC 3 cut(s) 5, 51, 413
BspCNI CTCAG 2 cut(s) 82, 640
BspFNI CGCG 4 cut(s) 257, 315, 366, 368
BspLI GGNNCC 1 cut(s) 362
BspPI GGATC 1 cut(s) 34
BspT104I TTCGAA 1 cut(s) 307
BspT107I GGYRCC 1 cut(s) 360
BssMI GATC 3 cut(s) 39, 387, 573
BssSI CACGAG 1 cut(s) 443
Bst2BI CACGAG 1 cut(s) 443
Bst4CI ACNGT 3 cut(s) 101, 355, 625
BstBAI YACGTR 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 307
BstC8I GCNNGC 2 cut(s) 370, 374
BstDEI CTNAG 2 cut(s) 69, 648
BstF5I GGATG 2 cut(s) 162, 544
BstFNI CGCG 4 cut(s) 257, 315, 366, 368
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 3 cut(s) 42, 390, 576
BstMBI GATC 3 cut(s) 39, 387, 573
BstMWI GCNNNNNNNGC 2 cut(s) 126, 135
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 4 cut(s) 257, 315, 366, 368
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 2 cut(s) 178, 642
BstXI CCANNNNNNTGG 1 cut(s) 445
BsuRI GGCC 3 cut(s) 5, 51, 413
BtrI CACGTC 3 cut(s) 244, 406, 475
BtsCI GGATG 2 cut(s) 162, 544
Cac8I GCNNGC 2 cut(s) 370, 374
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 2 cut(s) 411, 463
CseI GACGC 3 cut(s) 135, 246, 408
Csp6I GTAC 3 cut(s) 22, 361, 427
CviJI RGCY 6 cut(s) 5, 51, 67, 376, 413, 457
CviKI_1 RGCY 6 cut(s) 5, 51, 67, 376, 413, 457
CviQI GTAC 3 cut(s) 22, 361, 427
DdeI CTNAG 2 cut(s) 69, 648
DpnI GATC 3 cut(s) 41, 389, 575
DpnII GATC 3 cut(s) 39, 387, 573
DraIII CACNNNGTG 1 cut(s) 475
EciI GGCGGA 1 cut(s) 125
Eco47I GGWCC 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 463
FaiI YATR 2 cut(s) 601, 603
FaqI GGGAC 2 cut(s) 87, 244
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 2 cut(s) 121, 180
FokI GGATG 2 cut(s) 149, 551
Fsp4HI GCNGC 2 cut(s) 121, 180
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 367
GluI GCNGC 2 cut(s) 121, 180
HaeIII GGCC 3 cut(s) 5, 51, 413
HapII CCGG 1 cut(s) 48
HgaI GACGC 3 cut(s) 135, 246, 408
HhaI GCGC 1 cut(s) 368
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HincII GTYRAC 2 cut(s) 253, 451
HindII GTYRAC 2 cut(s) 253, 451
HinfI GANTC 4 cut(s) 228, 249, 338, 568
HpaII CCGG 1 cut(s) 48
HphI GGTGA 2 cut(s) 145, 286
Hpy166II GTNNAC 3 cut(s) 253, 433, 451
Hpy188I TCNGA 7 cut(s) 205, 248, 302, 394, 573, 621, 649
Hpy188III TCNNGA 3 cut(s) 275, 565, 632
Hpy8I GTNNAC 3 cut(s) 253, 433, 451
Hpy99I CGWCG 7 cut(s) 83, 86, 398, 424, 427, 455, 476
HpyAV CCTTC 2 cut(s) 176, 295
HpyCH4III ACNGT 3 cut(s) 101, 355, 625
HpyCH4IV ACGT 5 cut(s) 78, 243, 405, 429, 474
HpyCH4V TGCA 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 135
HpyF3I CTNAG 2 cut(s) 69, 648
HpySE526I ACGT 5 cut(s) 78, 243, 405, 429, 474
HspAI GCGC 1 cut(s) 366
KpnI GGTACC 1 cut(s) 364
Kzo9I GATC 3 cut(s) 39, 387, 573
LpnPI CCDG 4 cut(s) 61, 341, 394, 427
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 2 cut(s) 153, 596
MaeI CTAG 1 cut(s) 377
MaeII ACGT 5 cut(s) 78, 243, 405, 429, 474
MaeIII GTNAC 1 cut(s) 349
MalI GATC 3 cut(s) 41, 389, 575
MboI GATC 3 cut(s) 39, 387, 573
MboII GAAGA 5 cut(s) 178, 181, 313, 553, 647
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 2 cut(s) 331, 510
MluI ACGCGT 1 cut(s) 255
MlyI GAGTC 2 cut(s) 258, 332
MmeI TCCRAC 2 cut(s) 228, 417
MslI CAYNNNNRTG 1 cut(s) 443
MspI CCGG 1 cut(s) 48
Mva1269I GAATGC 1 cut(s) 26
MvnI CGCG 4 cut(s) 257, 315, 366, 368
MwoI GCNNNNNNNGC 2 cut(s) 126, 135
NdeII GATC 3 cut(s) 39, 387, 573
NlaIV GGNNCC 1 cut(s) 362
NmuCI GTSAC 1 cut(s) 349
NspV TTCGAA 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 443
PcsI WCGNNNNNNNCGW 3 cut(s) 41, 402, 441
PctI GAATGC 1 cut(s) 26
PfeI GAWTC 2 cut(s) 228, 568
Pfl23II CGTACG 1 cut(s) 426
PkrI GCNGC 2 cut(s) 122, 181
PleI GAGTC 2 cut(s) 257, 332
PpsI GAGTC 2 cut(s) 257, 332
Ppu21I YACGTR 1 cut(s) 430
PpuMI RGGWCCY 1 cut(s) 463
Psp5II RGGWCCY 1 cut(s) 463
PspLI CGTACG 1 cut(s) 426
PspN4I GGNNCC 1 cut(s) 362
PspPI GGNCC 2 cut(s) 411, 463
PspPPI RGGWCCY 1 cut(s) 463
RsaI GTAC 3 cut(s) 23, 362, 428
RsaNI GTAC 3 cut(s) 22, 361, 427
RseI CAYNNNNRTG 1 cut(s) 443
SalI GTCGAC 1 cut(s) 449
SatI GCNGC 2 cut(s) 121, 180
Sau3AI GATC 3 cut(s) 39, 387, 573
Sau96I GGNCC 2 cut(s) 411, 463
SchI GAGTC 2 cut(s) 258, 332
SduI GDGCHC 1 cut(s) 405
SetI ASST 9 cut(s) 81, 222, 246, 282, 378, 408, 432, 465, 477
SfaNI GCATC 2 cut(s) 153, 596
SfuI TTCGAA 1 cut(s) 307
SinI GGWCC 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 443
SmlI CTYRAG 2 cut(s) 458, 488
SmoI CTYRAG 2 cut(s) 458, 488
Sse9I AATT 2 cut(s) 331, 510
SsiI CCGC 7 cut(s) 123, 136, 156, 180, 313, 364, 533
SspMI CTAG 1 cut(s) 377
TaaI ACNGT 3 cut(s) 101, 355, 625
TaiI ACGT 5 cut(s) 81, 246, 408, 432, 477
TaqI TCGA 3 cut(s) 276, 307, 450
TasI AATT 2 cut(s) 331, 510
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 2 cut(s) 228, 568
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 349
TspGWI ACGGA 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.