MD02G1069000.v1.1

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013831
Physical Location & Seq
Forward (+)
5595715 .. 5597368
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1069000.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGGGATCAGATTCATACTTATGGTGAACAAGCAGGGGCAGACCCGACTTGCCCAATACTACGAATACCTCACTCTCGAAGAACGGCGAGCTCTTGAAGCTGAAATCGTCCGCAAATGCCTCGCCCGCAACGAGCAACAGTGTTCGTTTGTTGAGCACCGGAACTACAAAATTGTGTACAGGCGCTATGCTTCGTTGTTTTTTCTGGTTGGAGTCGACAATGATGAAAATGAGCTTGCAATTTTGGAATTCATACATCTGTTGGTTGAAACCATGGATCGCCATTTCGGCAATGTGTGTGAACTAGACATCATGTTCCATTTGGAGAAAGCGCATTTCATGCTAGAAGAAATGGTCATGAATGGATGCATTGTTGAGACGAGTAAGTCTAACATTCTGGCACCGATACAGCTGATGGACAAAATGACTTAG

Protein Analysis

144

Amino Acids

16.96

Weight (kDa)

5.54

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 3 - 142 9.9e-47 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013831)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19790
fragaria_vesca FvH4_1g06580 FvH4_5g22770
malus_domestica MD02G1069000.v1.1 MD15G1200600.v1.1
prunus_persica Prupe.7G216600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0092321
rosa_laevigata RLG00000016297
rosa_multiflora Rmu_sc0003289.1_g000015 Rmu_sc0003289.1_g000018
rosa_roxburghii Rroxscaffold_2G00149260
rosa_rugosa Rorug02G0024700
rosa_samantha Rh2AG070700 Rh2BG070200 Rh2CG071400 Rh2DG069200
rosa_wichuraiana Rw2G006160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 400
AccI GTMKAC 1 cut(s) 216
AciI CCGC 2 cut(s) 112, 127
AclWI GGATC 2 cut(s) 14, 285
AcsI RAATTY 1 cut(s) 248
AfaI GTAC 1 cut(s) 179
AgsI TTSAA 2 cut(s) 98, 269
AluBI AGCT 4 cut(s) 92, 101, 235, 412
AluI AGCT 4 cut(s) 92, 101, 235, 412
Alw21I GWGCWC 2 cut(s) 94, 159
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 2 cut(s) 14, 285
ApoI RAATTY 1 cut(s) 248
AspLEI GCGC 2 cut(s) 186, 334
AsuHPI GGTGA 1 cut(s) 37
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 94
Bbv12I GWGCWC 2 cut(s) 94, 159
BccI CCATC 1 cut(s) 409
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 305, 344
BfoI RGCGCY 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 288
BmiI GGNNCC 1 cut(s) 402
BmsI GCATC 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 400
BsiHKAI GWGCWC 2 cut(s) 94, 159
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 94, 159
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 2 cut(s) 6, 277
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 2 cut(s) 112, 127
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 402
BspPI GGATC 2 cut(s) 14, 285
BspT107I GGYRCC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 2 cut(s) 6, 277
BssT1I CCWWGG 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 340
BstAUI TGTACA 1 cut(s) 177
BstC8I GCNNGC 3 cut(s) 90, 127, 237
BstDEI CTNAG 1 cut(s) 429
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 371
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 2 cut(s) 186, 334
BstKTI GATC 2 cut(s) 9, 280
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 2 cut(s) 6, 277
BstMWI GCNNNNNNNGC 4 cut(s) 98, 126, 288, 340
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 3 cut(s) 90, 127, 237
CciI TCATGA 1 cut(s) 357
CfoI GCGC 2 cut(s) 186, 334
Csp6I GTAC 1 cut(s) 178
CviAII CATG 4 cut(s) 274, 313, 340, 358
CviJI RGCY 4 cut(s) 92, 101, 235, 412
CviKI_1 RGCY 4 cut(s) 92, 101, 235, 412
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 1 cut(s) 429
DpnI GATC 2 cut(s) 8, 279
DpnII GATC 2 cut(s) 6, 277
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
Ecl136II GAGCTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 371
EcoT38I GRGCYC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 4 cut(s) 277, 316, 343, 361
FaiI YATR 8 cut(s) 17, 23, 189, 254, 275, 314, 341, 359
FatI CATG 4 cut(s) 273, 312, 339, 357
FauI CCCGC 1 cut(s) 134
FblI GTMKAC 1 cut(s) 216
FokI GGATG 1 cut(s) 378
FriOI GRGCYC 1 cut(s) 94
FspBI CTAG 2 cut(s) 305, 344
GlaI GCGC 2 cut(s) 185, 333
HaeII RGCGCY 1 cut(s) 187
HapII CCGG 1 cut(s) 160
HhaI GCGC 2 cut(s) 186, 334
Hin1II CATG 4 cut(s) 277, 316, 343, 361
Hin6I GCGC 2 cut(s) 184, 332
HinP1I GCGC 2 cut(s) 184, 332
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 2 cut(s) 12, 213
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 4 cut(s) 28, 178, 217, 302
Hpy188I TCNGA 1 cut(s) 11
Hpy188III TCNNGA 3 cut(s) 77, 95, 358
Hpy8I GTNNAC 4 cut(s) 28, 178, 217, 302
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4V TGCA 2 cut(s) 239, 369
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 126, 288, 340
HpyF3I CTNAG 1 cut(s) 429
Hsp92II CATG 4 cut(s) 277, 316, 343, 361
HspAI GCGC 2 cut(s) 184, 332
Kzo9I GATC 2 cut(s) 6, 277
LpnPI CCDG 5 cut(s) 20, 166, 173, 191, 383
LweI GCATC 1 cut(s) 356
MaeI CTAG 2 cut(s) 305, 344
MalI GATC 2 cut(s) 8, 279
MboI GATC 2 cut(s) 6, 277
MboII GAAGA 2 cut(s) 92, 359
MhlI GDGCHC 2 cut(s) 94, 159
MluCI AATT 3 cut(s) 171, 240, 248
MlyI GAGTC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 2 cut(s) 80, 131
Mph1103I ATGCAT 1 cut(s) 371
MslI CAYNNNNRTG 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 1 cut(s) 160
MwoI GCNNNNNNNGC 4 cut(s) 98, 126, 288, 340
NcoI CCATGG 1 cut(s) 273
NdeII GATC 2 cut(s) 6, 277
NlaIII CATG 4 cut(s) 277, 316, 343, 361
NlaIV GGNNCC 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 371
PagI TCATGA 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PfeI GAWTC 1 cut(s) 12
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 412
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 20
SacI GAGCTC 1 cut(s) 94
SalI GTCGAC 1 cut(s) 215
Sau3AI GATC 2 cut(s) 6, 277
SchI GAGTC 1 cut(s) 222
SduI GDGCHC 2 cut(s) 94, 159
SetI ASST 5 cut(s) 72, 94, 103, 237, 414
SfaNI GCATC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 20
Sse9I AATT 3 cut(s) 171, 240, 248
SsiI CCGC 2 cut(s) 112, 127
SspMI CTAG 2 cut(s) 305, 344
SstI GAGCTC 1 cut(s) 94
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 78, 216
TasI AATT 3 cut(s) 171, 240, 248
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 1 cut(s) 12
TscAI CASTG 1 cut(s) 146
TspDTI ATGAA 5 cut(s) 4, 240, 241, 328, 374
TspRI CASTG 1 cut(s) 146
XapI RAATTY 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 2 cut(s) 305, 344
Zsp2I ATGCAT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.