MD15G1200600.v1.1

Belongs to the adaptor complexes small subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013831
Physical Location & Seq
Forward (+)
15958080 .. 15959657
1578 bp
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UTR
Exon/CDS
Intron
MD15G1200600.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGGGATCAGATTTATACTGATGGTGAACAAACAGGGGCAGACCCGGCTTGCCCAATACTACGAATACCTCACTCTTGAAGAACGGCGAGCTCTTGAACCTGAAATCGTCCGCAAATGCCTCGCCCGCACAGAGCAACAGTGTTCGTTTGTTGAGCATCGGAACTACAAAATTGTGTACAGGCGCTATGCTTCGTTGTTTTTCCTTGTTGGAGTTGACAATGATGAAAATGAGCTTGCAATTTTGGAGTTCATACATCTGTTGGTTGAGACTATGGACCGCCATTTCGGCAATGTGTGTGAACTAGACATCATGTTCCATTTGGAGAAAGCGCATTTCATGCTAGAAGAAATGGTCATGAATGGATGCATTGTTGAGACGAACAAGTCTAGCATTCTGGCACCGATACAGCTGATGGACAAGATGAATTAG

Protein Analysis

144

Amino Acids

16.98

Weight (kDa)

5.54

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 3 - 142 3e-46 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013831)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19790
fragaria_vesca FvH4_1g06580 FvH4_5g22770
malus_domestica MD02G1069000.v1.1 MD15G1200600.v1.1
prunus_persica Prupe.7G216600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0092321
rosa_laevigata RLG00000016297
rosa_multiflora Rmu_sc0003289.1_g000015 Rmu_sc0003289.1_g000018
rosa_roxburghii Rroxscaffold_2G00149260
rosa_rugosa Rorug02G0024700
rosa_samantha Rh2AG070700 Rh2BG070200 Rh2CG071400 Rh2DG069200
rosa_wichuraiana Rw2G006160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 400
AciI CCGC 3 cut(s) 112, 127, 280
AclWI GGATC 1 cut(s) 14
AfaI GTAC 1 cut(s) 179
AgsI TTSAA 2 cut(s) 80, 98
AluBI AGCT 3 cut(s) 92, 235, 412
AluI AGCT 3 cut(s) 92, 235, 412
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 2 cut(s) 263, 371
AlwI GGATC 1 cut(s) 14
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AspLEI GCGC 2 cut(s) 186, 334
AspS9I GGNCC 1 cut(s) 277
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 94
BccI CCATC 2 cut(s) 16, 409
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 263, 371
BfaI CTAG 3 cut(s) 305, 344, 390
BfoI RGCGCY 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 288
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 1 cut(s) 46
BmsI GCATC 2 cut(s) 166, 356
BpuMI CCSGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 298
BseGI GGATG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 46
BsmAI GTCTC 2 cut(s) 263, 371
BsmBI CGTCTC 1 cut(s) 371
BsmI GAATGC 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 3 cut(s) 112, 127, 280
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 402
BspPI GGATC 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 177
BssMI GATC 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 340
BstAUI TGTACA 1 cut(s) 177
BstC8I GCNNGC 4 cut(s) 51, 90, 127, 237
BstF5I GGATG 1 cut(s) 371
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 2 cut(s) 186, 334
BstKTI GATC 1 cut(s) 9
BstMAI GTCTC 2 cut(s) 263, 371
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 4 cut(s) 46, 126, 288, 340
BstSCI CCNGG 1 cut(s) 44
BtsCI GGATG 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 4 cut(s) 51, 90, 127, 237
CciI TCATGA 1 cut(s) 357
CfoI GCGC 2 cut(s) 186, 334
Cfr13I GGNCC 1 cut(s) 277
Csp6I GTAC 1 cut(s) 178
CviAII CATG 3 cut(s) 313, 340, 358
CviJI RGCY 4 cut(s) 49, 92, 235, 412
CviKI_1 RGCY 4 cut(s) 49, 92, 235, 412
CviQI GTAC 1 cut(s) 178
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
Ecl136II GAGCTC 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 92
EcoT22I ATGCAT 1 cut(s) 371
EcoT38I GRGCYC 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 3 cut(s) 316, 343, 361
FaiI YATR 7 cut(s) 17, 189, 254, 275, 314, 341, 359
FatI CATG 3 cut(s) 312, 339, 357
FauI CCCGC 1 cut(s) 134
FokI GGATG 1 cut(s) 378
FriOI GRGCYC 1 cut(s) 94
FspBI CTAG 3 cut(s) 305, 344, 390
GlaI GCGC 2 cut(s) 185, 333
HaeII RGCGCY 1 cut(s) 187
HapII CCGG 1 cut(s) 46
HhaI GCGC 2 cut(s) 186, 334
Hin1II CATG 3 cut(s) 316, 343, 361
Hin6I GCGC 2 cut(s) 184, 332
HinP1I GCGC 2 cut(s) 184, 332
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HpaII CCGG 1 cut(s) 46
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 4 cut(s) 28, 178, 217, 302
Hpy188I TCNGA 2 cut(s) 11, 162
Hpy188III TCNNGA 3 cut(s) 77, 95, 358
Hpy8I GTNNAC 4 cut(s) 28, 178, 217, 302
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4V TGCA 2 cut(s) 239, 369
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 126, 288, 340
Hsp92II CATG 3 cut(s) 316, 343, 361
HspAI GCGC 2 cut(s) 184, 332
Kzo9I GATC 1 cut(s) 6
LpnPI CCDG 5 cut(s) 20, 59, 114, 166, 383
LweI GCATC 2 cut(s) 166, 356
MaeI CTAG 3 cut(s) 305, 344, 390
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 2 cut(s) 92, 359
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 3 cut(s) 171, 240, 427
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 2 cut(s) 80, 131
Mph1103I ATGCAT 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 46
Mva1269I GAATGC 1 cut(s) 393
MwoI GCNNNNNNNGC 4 cut(s) 46, 126, 288, 340
NciI CCSGG 1 cut(s) 46
NdeII GATC 1 cut(s) 6
NlaIII CATG 3 cut(s) 316, 343, 361
NlaIV GGNNCC 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 371
PagI TCATGA 1 cut(s) 357
PctI GAATGC 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 402
PspPI GGNCC 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 412
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SacI GAGCTC 1 cut(s) 94
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 1 cut(s) 94
SetI ASST 5 cut(s) 72, 94, 103, 237, 414
SfaNI GCATC 2 cut(s) 166, 356
SinI GGWCC 1 cut(s) 277
Sse9I AATT 3 cut(s) 171, 240, 427
SsiI CCGC 3 cut(s) 112, 127, 280
SspMI CTAG 3 cut(s) 305, 344, 390
SstI GAGCTC 1 cut(s) 94
StyD4I CCNGG 1 cut(s) 44
TaaI ACNGT 1 cut(s) 141
TasI AATT 3 cut(s) 171, 240, 427
TatI WGTACW 1 cut(s) 177
TscAI CASTG 1 cut(s) 146
TspDTI ATGAA 4 cut(s) 240, 241, 328, 374
TspRI CASTG 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 277
XspI CTAG 3 cut(s) 305, 344, 390
Zsp2I ATGCAT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.