MD02G1074400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014440
Physical Location & Seq
Reverse (-)
5966859 .. 5969541
2683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1074400.v1.1.491

Sequence Viewer

Length: 918 bp
ATGCCGAAGGTGAAGCAAATTCTAGTAGCAATCTCAGTATTGTTGGCGGTGTTAGCGTGCGTGCCAATCTCTGAGTGTGCTAAGAAGCCGGCGGCCGGCGCCAGGAAGGAAGACATTCCTTTCATCAAATGCCAAGTCTGCGAGAAGCTCGCAGTTCAGCTGCACCAGCAAGTCGAGAAGAAGCGAGCTGAGATCGCCCCAAAGAAGATCTCGGAGTATCAGATTATTGAAATTTCGGAGAATGTGTGTAATTTGAAGAAGCAGGAAGCGGATTGGATTTTGCAGATTGATATAGTTGAGAAAGGAGACAAGTTGGAGTTGGTGGATCAAGGTTCAGAAGGACAGTGTAACTCCGAATGCAAGACAATCGAGCGAGCTTGTCAGGAGGTTTTGGGGTATTCTGATACTGATGTTGCCGAATATCTATATACATCCAAGCCTGACCTTGATTCATTGGTTAATTATCTATGCAAAGACCTAACTAAAGCGTGCAGTACCAAGCCTCCCCCAGTTCCTAAGAATAGGATTCCGGGGGAAGCTTTCGTGGCCAAGTCAGAGAAAGAAGCTGAAATGGAAAAGATGATGAAATCTATGGAGGGCATGCCAGGAGCCCCGGGCATGAAAATGTACTCGAGAGATGATTTGATGAACATGAATAATTTTGGTGGCGAGGATGCTGATGATGAAGATGATGACGATGATGAGACTCGGTTACCCTCGAATTTGGGAAAAATAATGAGAGAAAAAGAGAGCGCAAAGAAAGACTTGAAACAGAAGATCACCACCGGAATCGCGAAGACTAGAGAGACACTAAAGAAGCATGCAAACAAAGTCTCTAACTGGGTACGGCAAACGTGGCGGGGACCGAAAAAGACGGCTTCGAAGAAGAGTACAAAGGGAAACAAGGGAGAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

34.08

Weight (kDa)

7.91

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29520
fragaria_vesca FvH4_1g07000 FvH4_1g07000
malus_domestica MD02G1074400.v1.1 MD15G1203900.v1.1
prunus_persica Prupe.7G213500_v2.0.a1
pyrus_communis pycom02g05880
rosa_chinensis RchiOBHm_Chr2g0092951
rosa_laevigata RLG00000016352
rosa_multiflora Rmu_sc0000965.1_g000016
rosa_roxburghii Rroxscaffold_2G00148680
rosa_rugosa Rorug02G0030600
rosa_samantha Rh2AG076600 Rh2BG077900 Rh2CG080100 Rh2DG075200
rosa_wichuraiana Rw2G006960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccII CGCG 1 cut(s) 794
AciI CCGC 4 cut(s) 47, 92, 269, 859
AclWI GGATC 1 cut(s) 333
AcoI YGGCCR 2 cut(s) 93, 546
AcsI RAATTY 3 cut(s) 18, 231, 721
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 4 cut(s) 496, 629, 846, 892
AfiI CCNNNNNNNGG 2 cut(s) 95, 102
AgsI TTSAA 3 cut(s) 230, 256, 769
AjnI CCWGG 2 cut(s) 101, 604
AluBI AGCT 7 cut(s) 148, 160, 188, 377, 539, 566, 913
AluI AGCT 7 cut(s) 148, 160, 188, 377, 539, 566, 913
Alw26I GTCTC 4 cut(s) 300, 698, 800, 838
AlwI GGATC 1 cut(s) 333
Ama87I CYCGRG 2 cut(s) 613, 631
AoxI GGCC 2 cut(s) 93, 546
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 3 cut(s) 18, 231, 721
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
Asp700I GAANNNNTTC 1 cut(s) 114
AspLEI GCGC 2 cut(s) 101, 755
AspS9I GGNCC 1 cut(s) 863
AsuC2I CCSGG 3 cut(s) 531, 614, 615
AsuHPI GGTGA 2 cut(s) 22, 772
AsuII TTCGAA 1 cut(s) 881
AvaI CYCGRG 2 cut(s) 613, 631
AvaII GGWCC 1 cut(s) 863
BaeI ACNNNNGTAYC 2 cut(s) 396, 429
BalI TGGCCA 1 cut(s) 548
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 613
BbsI GAAGAC 2 cut(s) 117, 803
BbvI GCAGC 1 cut(s) 147
BceAI ACGGC 2 cut(s) 863, 891
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciT130I CCWGG 2 cut(s) 103, 606
BcnI CCSGG 3 cut(s) 531, 614, 615
BcoDI GTCTC 4 cut(s) 300, 698, 800, 838
BfaI CTAG 2 cut(s) 23, 801
BfoI RGCGCY 1 cut(s) 102
BglII AGATCT 1 cut(s) 207
BisI GCNGC 2 cut(s) 93, 161
BlsI GCNGC 2 cut(s) 94, 162
Bme1390I CCNGG 5 cut(s) 103, 531, 606, 614, 615
Bme18I GGWCC 1 cut(s) 863
BmeT110I CYCGRG 2 cut(s) 613, 631
BmgT120I GGNCC 1 cut(s) 863
BmiI GGNNCC 3 cut(s) 100, 610, 864
BmrFI CCNGG 5 cut(s) 103, 531, 606, 614, 615
BmrI ACTGGG 2 cut(s) 503, 850
BmsI GCATC 1 cut(s) 664
BmuI ACTGGG 2 cut(s) 503, 850
BpiI GAAGAC 2 cut(s) 117, 803
Bpu14I TTCGAA 1 cut(s) 881
BpuMI CCSGG 3 cut(s) 531, 614, 615
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 530, 612, 613
BsaWI WCCGGW 1 cut(s) 785
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 487, 517
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 102
Bse118I RCCGGY 2 cut(s) 88, 95
Bse1I ACTGG 2 cut(s) 509, 845
BseBI CCWGG 2 cut(s) 103, 606
BseDI CCNNGG 3 cut(s) 530, 612, 613
BseGI GGATG 2 cut(s) 431, 679
BseLI CCNNNNNNNGG 2 cut(s) 95, 102
BseMII CTCAG 3 cut(s) 48, 63, 180
BseNI ACTGG 2 cut(s) 509, 845
BseX3I CGGCCG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 147
BsgI GTGCAG 2 cut(s) 146, 511
Bsh1236I CGCG 1 cut(s) 794
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 95, 548
BshNI GGYRCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 96
BsiHKCI CYCGRG 2 cut(s) 613, 631
BsiSI CCGG 5 cut(s) 89, 96, 530, 614, 786
BslFI GGGAC 1 cut(s) 876
BslI CCNNNNNNNGG 2 cut(s) 95, 102
BsmAI GTCTC 4 cut(s) 300, 698, 800, 838
BsmFI GGGAC 1 cut(s) 876
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 2 cut(s) 95, 548
BsoBI CYCGRG 2 cut(s) 613, 631
Bsp119I TTCGAA 1 cut(s) 881
Bsp1286I GDGCHC 1 cut(s) 613
Bsp143I GATC 4 cut(s) 192, 207, 325, 777
Bsp68I TCGCGA 1 cut(s) 794
BspACI CCGC 4 cut(s) 47, 92, 269, 859
BspANI GGCC 2 cut(s) 95, 548
BspCNI CTCAG 3 cut(s) 47, 64, 181
BspFNI CGCG 1 cut(s) 794
BspLI GGNNCC 3 cut(s) 100, 610, 864
BspPI GGATC 1 cut(s) 333
BspT104I TTCGAA 1 cut(s) 881
BspT107I GGYRCC 1 cut(s) 98
BsrFI RCCGGY 2 cut(s) 88, 95
BsrI ACTGG 2 cut(s) 509, 845
BssAI RCCGGY 2 cut(s) 88, 95
BssECI CCNNGG 3 cut(s) 530, 612, 613
BssMI GATC 4 cut(s) 192, 207, 325, 777
BssNI GRCGYC 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 103, 606
Bst4CI ACNGT 1 cut(s) 345
Bst6I CTCTTC 1 cut(s) 881
BstACI GRCGYC 1 cut(s) 99
BstBI TTCGAA 1 cut(s) 881
BstDEI CTNAG 5 cut(s) 34, 72, 81, 189, 516
BstEII GGTNACC 1 cut(s) 711
BstF5I GGATG 2 cut(s) 431, 679
BstFNI CGCG 1 cut(s) 794
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 101, 755
BstKTI GATC 4 cut(s) 195, 210, 328, 780
BstMAI GTCTC 4 cut(s) 300, 698, 800, 838
BstMBI GATC 4 cut(s) 192, 207, 325, 777
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 7 cut(s) 53, 98, 138, 166, 194, 545, 856
BstNI CCWGG 2 cut(s) 103, 606
BstNSI RCATGY 2 cut(s) 604, 824
BstPI GGTNACC 1 cut(s) 711
BstSCI CCNGG 5 cut(s) 101, 529, 604, 612, 613
BstUI CGCG 1 cut(s) 794
BstV1I GCAGC 1 cut(s) 147
BstV2I GAAGAC 2 cut(s) 117, 803
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BstZI CGGCCG 1 cut(s) 93
BsuRI GGCC 2 cut(s) 95, 548
BtsCI GGATG 2 cut(s) 431, 679
BtsIMutI CAGTG 1 cut(s) 350
BtuMI TCGCGA 1 cut(s) 794
CfoI GCGC 2 cut(s) 101, 755
Cfr10I RCCGGY 2 cut(s) 88, 95
Cfr13I GGNCC 1 cut(s) 863
Cfr9I CCCGGG 1 cut(s) 613
Csp6I GTAC 4 cut(s) 495, 628, 845, 891
CviAII CATG 4 cut(s) 601, 619, 652, 821
CviQI GTAC 4 cut(s) 495, 628, 845, 891
DdeI CTNAG 5 cut(s) 34, 72, 81, 189, 516
DinI GGCGCC 1 cut(s) 100
DpnI GATC 4 cut(s) 194, 209, 327, 779
DpnII GATC 4 cut(s) 192, 207, 325, 777
EaeI YGGCCR 2 cut(s) 93, 546
EagI CGGCCG 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 881
EarI CTCTTC 1 cut(s) 881
EclXI CGGCCG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 863
Eco52I CGGCCG 1 cut(s) 93
Eco88I CYCGRG 2 cut(s) 613, 631
Eco91I GGTNACC 1 cut(s) 711
EcoO65I GGTNACC 1 cut(s) 711
EcoRII CCWGG 2 cut(s) 101, 604
EcoT38I GRGCYC 1 cut(s) 613
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
FaeI CATG 4 cut(s) 604, 622, 655, 824
FaiI YATR 9 cut(s) 293, 427, 429, 469, 593, 602, 620, 653, 822
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 1 cut(s) 876
FatI CATG 4 cut(s) 600, 618, 651, 820
FauI CCCGC 1 cut(s) 852
Fnu4HI GCNGC 2 cut(s) 93, 161
FokI GGATG 2 cut(s) 418, 686
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 2 cut(s) 93, 161
FspBI CTAG 2 cut(s) 23, 801
GlaI GCGC 2 cut(s) 100, 754
GluI GCNGC 2 cut(s) 93, 161
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 2 cut(s) 95, 548
HapII CCGG 5 cut(s) 89, 96, 530, 614, 786
HhaI GCGC 2 cut(s) 101, 755
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 4 cut(s) 604, 622, 655, 824
Hin6I GCGC 2 cut(s) 99, 753
HinP1I GCGC 2 cut(s) 99, 753
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 4 cut(s) 449, 526, 706, 789
HpaII CCGG 5 cut(s) 89, 96, 530, 614, 786
HphI GGTGA 2 cut(s) 22, 772
Hpy188I TCNGA 8 cut(s) 73, 214, 222, 238, 337, 355, 403, 556
Hpy188III TCNNGA 4 cut(s) 175, 383, 633, 793
HpyAV CCTTC 2 cut(s) 100, 332
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 1 cut(s) 854
HpyCH4V TGCA 6 cut(s) 163, 283, 360, 471, 492, 824
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 98, 138, 166, 194, 545, 856
HpyF3I CTNAG 5 cut(s) 34, 72, 81, 189, 516
HpySE526I ACGT 1 cut(s) 854
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 4 cut(s) 604, 622, 655, 824
HspAI GCGC 2 cut(s) 99, 753
KasI GGCGCC 1 cut(s) 98
KroI GCCGGC 2 cut(s) 88, 95
KroNI GCCGGC 2 cut(s) 90, 97
Kzo9I GATC 4 cut(s) 192, 207, 325, 777
LmnI GCTCC 1 cut(s) 608
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 1 cut(s) 664
MaeI CTAG 2 cut(s) 23, 801
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 2 cut(s) 347, 711
MalI GATC 4 cut(s) 194, 209, 327, 779
MboI GATC 4 cut(s) 192, 207, 325, 777
MboII GAAGA 9 cut(s) 122, 190, 217, 268, 698, 787, 808, 895, 898
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 613
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 6 cut(s) 18, 231, 250, 460, 658, 721
MluNI TGGCCA 1 cut(s) 548
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 5 cut(s) 379, 513, 589, 664, 727
Mox20I TGGCCA 1 cut(s) 548
MroNI GCCGGC 2 cut(s) 88, 95
MroXI GAANNNNTTC 1 cut(s) 114
MscI TGGCCA 1 cut(s) 548
MseI TTAA 1 cut(s) 459
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 5 cut(s) 89, 96, 530, 614, 786
MspR9I CCNGG 5 cut(s) 103, 531, 606, 614, 615
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 2 cut(s) 103, 606
MvnI CGCG 1 cut(s) 794
MwoI GCNNNNNNNGC 7 cut(s) 53, 98, 138, 166, 194, 545, 856
NaeI GCCGGC 2 cut(s) 90, 97
NarI GGCGCC 1 cut(s) 99
NciI CCSGG 3 cut(s) 531, 614, 615
NdeII GATC 4 cut(s) 192, 207, 325, 777
NgoMIV GCCGGC 2 cut(s) 88, 95
NlaIII CATG 4 cut(s) 604, 622, 655, 824
NlaIV GGNNCC 3 cut(s) 100, 610, 864
NruI TCGCGA 1 cut(s) 794
NspI RCATGY 2 cut(s) 604, 824
NspV TTCGAA 1 cut(s) 881
PaeI GCATGC 2 cut(s) 604, 824
PaeR7I CTCGAG 1 cut(s) 631
PctI GAATGC 1 cut(s) 362
PdiI GCCGGC 2 cut(s) 90, 97
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 3 cut(s) 449, 526, 789
PkrI GCNGC 2 cut(s) 94, 162
PleI GAGTC 1 cut(s) 700
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 700
Psp6I CCWGG 2 cut(s) 101, 604
PspEI GGTNACC 1 cut(s) 711
PspGI CCWGG 2 cut(s) 101, 604
PspN4I GGNNCC 3 cut(s) 100, 610, 864
PspPI GGNCC 1 cut(s) 863
PsuI RGATCY 1 cut(s) 207
PvuII CAGCTG 1 cut(s) 160
RruI TCGCGA 1 cut(s) 794
RsaI GTAC 4 cut(s) 496, 629, 846, 892
RsaNI GTAC 4 cut(s) 495, 628, 845, 891
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 2 cut(s) 93, 161
Sau3AI GATC 4 cut(s) 192, 207, 325, 777
Sau96I GGNCC 1 cut(s) 863
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 5 cut(s) 103, 531, 606, 614, 615
SduI GDGCHC 1 cut(s) 613
SfaNI GCATC 1 cut(s) 664
SfoI GGCGCC 1 cut(s) 100
Sfr274I CTCGAG 1 cut(s) 631
SfuI TTCGAA 1 cut(s) 881
SinI GGWCC 1 cut(s) 863
SlaI CTCGAG 1 cut(s) 631
SmaI CCCGGG 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
SphI GCATGC 2 cut(s) 604, 824
Sse9I AATT 6 cut(s) 18, 231, 250, 460, 658, 721
SsiI CCGC 4 cut(s) 47, 92, 269, 859
SspDI GGCGCC 1 cut(s) 98
SspMI CTAG 2 cut(s) 23, 801
StyD4I CCNGG 5 cut(s) 101, 529, 604, 612, 613
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 1 cut(s) 857
TaqI TCGA 5 cut(s) 174, 369, 632, 719, 881
TaqII GACCGA 1 cut(s) 880
TasI AATT 6 cut(s) 18, 231, 250, 460, 658, 721
TatI WGTACW 2 cut(s) 627, 890
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 3 cut(s) 449, 526, 789
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 1 cut(s) 350
TseI GCWGC 1 cut(s) 160
TspDTI ATGAA 7 cut(s) 112, 441, 599, 635, 662, 668, 699
TspMI CCCGGG 1 cut(s) 613
TspRI CASTG 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 863
XapI RAATTY 3 cut(s) 18, 231, 721
XceI RCATGY 2 cut(s) 604, 824
XhoI CTCGAG 1 cut(s) 631
XmaI CCCGGG 1 cut(s) 613
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 2 cut(s) 23, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.