MD15G1203900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014440
Physical Location & Seq
Reverse (-)
16213694 .. 16216336
2643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1203900.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGCGAAGCTGAAGCAAATTCTAGTAGCTATCTCAGTGTTGTTGGCGGTGTTAGCGTCCGTGCCATTCTCCGAGTGTGTTAAGAAGCCGGCGGCCGGCGCCAGGAAGGAAGACATTCCTTTCATCAAATGCCAAGTCTGCGAGAAGCTCGCATCTCAGCTGCACCAGCAAGTCGAGAAGAAGCGAGCTGAGATCGCCCCAAAGAAGATCTCGGAGTATCAGATCATTGAGATTTCGGAGAATGTCTGTAATTTGAAGAAGCAGGAAGCTGATTGGATTTTGCAGATTGATATAGTTGAGAAAGGAGATAAGCTGGAGTTGGTGGATCAAGGTTCTGAAGGACAATGTAATTCCGAATGCAAGACAATCGAGCGAGCTTGTCAGGAGGTTTTGGGGTATTCTGATACAGATGTTGCAGAATATCTATATACATCCAAGCCTGACCTTGGTTCATTGGTGAATTATCTATGCAAAGACCTCACTAAAGCATGCAGTACCAAGCCTCCCCCAGTTCCTAAGAATAGGATTCCTGGGGAAGCTTTCGTGGCCAAGTCGGAGAAAGAAGCTGAAATGGAAAGGATAATGAAATCTATGGAGGGTATGCCAGGAGCCCCAGGCATGAAGATGTACTCAAAAGATGATTTGATGAACATGAATAATTTTGGCGGCAAAGATGCTGATGATGAAGATGATGATGACGATGATGAGACTCTGTTACCCTCGAATTTGGGAAAAATCATGAGAGAAAAAGAGAGTGCAAAGAATGACTTGAAACAGAAGATCACCACCGGAATTGTGAAGACCAGAGAGACACTAAAGAAGCATGCAAACAAAGTCTCTAACTGGTTCCGGCAAACGTGGCGGCGAGTGAAAAAGACGGCTTCAGAGAAGGGTACGAAGGGCAACAAGGGAGAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.24

Weight (kDa)

7.44

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29520
fragaria_vesca FvH4_1g07000 FvH4_1g07000
malus_domestica MD02G1074400.v1.1 MD15G1203900.v1.1
prunus_persica Prupe.7G213500_v2.0.a1
pyrus_communis pycom02g05880
rosa_chinensis RchiOBHm_Chr2g0092951
rosa_laevigata RLG00000016352
rosa_multiflora Rmu_sc0000965.1_g000016
rosa_roxburghii Rroxscaffold_2G00148680
rosa_rugosa Rorug02G0030600
rosa_samantha Rh2AG076600 Rh2BG077900 Rh2CG080100 Rh2DG075200
rosa_wichuraiana Rw2G006960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AciI CCGC 4 cut(s) 47, 92, 666, 862
AclWI GGATC 1 cut(s) 333
AcoI YGGCCR 2 cut(s) 93, 546
AcsI RAATTY 2 cut(s) 18, 724
AcuI CTGAAG 3 cut(s) 32, 357, 867
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 3 cut(s) 496, 629, 895
AfiI CCNNNNNNNGG 3 cut(s) 95, 102, 446
AgsI TTSAA 2 cut(s) 256, 772
AjnI CCWGG 4 cut(s) 101, 529, 604, 613
Alw26I GTCTC 3 cut(s) 701, 803, 841
AlwI GGATC 1 cut(s) 333
AoxI GGCC 2 cut(s) 93, 546
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 2 cut(s) 18, 724
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
Asp700I GAANNNNTTC 1 cut(s) 114
AspLEI GCGC 1 cut(s) 101
AsuHPI GGTGA 2 cut(s) 469, 775
BaeI ACNNNNGTAYC 2 cut(s) 396, 429
BalI TGGCCA 1 cut(s) 548
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 613
BbsI GAAGAC 2 cut(s) 117, 806
BbvI GCAGC 1 cut(s) 147
BceAI ACGGC 1 cut(s) 894
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciT130I CCWGG 4 cut(s) 103, 531, 606, 615
BcoDI GTCTC 3 cut(s) 701, 803, 841
BfaI CTAG 1 cut(s) 23
BfoI RGCGCY 1 cut(s) 102
BglII AGATCT 1 cut(s) 207
BisI GCNGC 4 cut(s) 93, 161, 667, 863
BlsI GCNGC 4 cut(s) 94, 162, 668, 864
Bme1390I CCNGG 4 cut(s) 103, 531, 606, 615
BmiI GGNNCC 3 cut(s) 100, 610, 848
BmrFI CCNGG 4 cut(s) 103, 531, 606, 615
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 2 cut(s) 161, 664
BmuI ACTGGG 1 cut(s) 503
BpiI GAAGAC 2 cut(s) 117, 806
BpmI CTGGAG 1 cut(s) 335
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 445, 530, 613
BsaWI WCCGGW 1 cut(s) 788
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 102, 446
Bse118I RCCGGY 2 cut(s) 88, 95
Bse1I ACTGG 2 cut(s) 509, 848
BseBI CCWGG 4 cut(s) 103, 531, 606, 615
BseDI CCNNGG 3 cut(s) 445, 530, 613
BseGI GGATG 1 cut(s) 431
BseLI CCNNNNNNNGG 3 cut(s) 95, 102, 446
BseMII CTCAG 3 cut(s) 48, 170, 180
BseNI ACTGG 2 cut(s) 509, 848
BseX3I CGGCCG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 95, 548
BshNI GGYRCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 96
BsiSI CCGG 4 cut(s) 89, 96, 789, 850
BslI CCNNNNNNNGG 3 cut(s) 95, 102, 446
BsmAI GTCTC 3 cut(s) 701, 803, 841
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 2 cut(s) 95, 548
Bsp1286I GDGCHC 1 cut(s) 613
Bsp143I GATC 5 cut(s) 192, 207, 222, 325, 780
BspACI CCGC 4 cut(s) 47, 92, 666, 862
BspANI GGCC 2 cut(s) 95, 548
BspCNI CTCAG 3 cut(s) 47, 169, 181
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 3 cut(s) 100, 610, 848
BspPI GGATC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 98
BsrFI RCCGGY 2 cut(s) 88, 95
BsrI ACTGG 2 cut(s) 509, 848
BssAI RCCGGY 2 cut(s) 88, 95
BssECI CCNNGG 3 cut(s) 445, 530, 613
BssMI GATC 5 cut(s) 192, 207, 222, 325, 780
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 4 cut(s) 103, 531, 606, 615
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 7 cut(s) 90, 97, 150, 186, 375, 490, 825
BstDEI CTNAG 4 cut(s) 34, 156, 189, 516
BstF5I GGATG 1 cut(s) 431
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 5 cut(s) 195, 210, 225, 328, 783
BstMAI GTCTC 3 cut(s) 701, 803, 841
BstMBI GATC 5 cut(s) 192, 207, 222, 325, 780
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 7 cut(s) 53, 98, 138, 166, 194, 545, 859
BstNI CCWGG 4 cut(s) 103, 531, 606, 615
BstNSI RCATGY 2 cut(s) 492, 827
BstSCI CCNGG 4 cut(s) 101, 529, 604, 613
BstV1I GCAGC 1 cut(s) 147
BstV2I GAAGAC 2 cut(s) 117, 806
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BstZI CGGCCG 1 cut(s) 93
BsuRI GGCC 2 cut(s) 95, 548
BtsCI GGATG 1 cut(s) 431
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 7 cut(s) 90, 97, 150, 186, 375, 490, 825
CciI TCATGA 1 cut(s) 738
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 2 cut(s) 88, 95
CseI GACGC 1 cut(s) 45
Csp6I GTAC 3 cut(s) 495, 628, 894
CspCI CAANNNNNGTGG 2 cut(s) 775, 810
CviAII CATG 5 cut(s) 489, 619, 652, 739, 824
CviQI GTAC 3 cut(s) 495, 628, 894
DdeI CTNAG 4 cut(s) 34, 156, 189, 516
DinI GGCGCC 1 cut(s) 100
DpnI GATC 5 cut(s) 194, 209, 224, 327, 782
DpnII GATC 5 cut(s) 192, 207, 222, 325, 780
EaeI YGGCCR 2 cut(s) 93, 546
EagI CGGCCG 1 cut(s) 93
EclXI CGGCCG 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 613
Eco52I CGGCCG 1 cut(s) 93
Eco57I CTGAAG 3 cut(s) 32, 357, 867
EcoRII CCWGG 4 cut(s) 101, 529, 604, 613
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 613
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 5 cut(s) 492, 622, 655, 742, 827
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FatI CATG 5 cut(s) 488, 618, 651, 738, 823
Fnu4HI GCNGC 4 cut(s) 93, 161, 667, 863
FokI GGATG 1 cut(s) 418
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 4 cut(s) 93, 161, 667, 863
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 100
GluI GCNGC 4 cut(s) 93, 161, 667, 863
GsuI CTGGAG 1 cut(s) 335
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 2 cut(s) 95, 548
HapII CCGG 4 cut(s) 89, 96, 789, 850
HgaI GACGC 1 cut(s) 45
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 5 cut(s) 492, 622, 655, 742, 827
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 2 cut(s) 526, 709
HpaII CCGG 4 cut(s) 89, 96, 789, 850
HphI GGTGA 2 cut(s) 469, 775
Hpy188I TCNGA 9 cut(s) 73, 214, 222, 238, 337, 355, 403, 556, 886
Hpy188III TCNNGA 3 cut(s) 175, 383, 739
HpyAV CCTTC 4 cut(s) 100, 332, 883, 892
HpyCH4IV ACGT 1 cut(s) 857
HpyCH4V TGCA 8 cut(s) 163, 283, 360, 416, 471, 492, 758, 827
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 98, 138, 166, 194, 545, 859
HpyF3I CTNAG 4 cut(s) 34, 156, 189, 516
HpySE526I ACGT 1 cut(s) 857
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 5 cut(s) 492, 622, 655, 742, 827
HspAI GCGC 1 cut(s) 99
KasI GGCGCC 1 cut(s) 98
KroI GCCGGC 2 cut(s) 88, 95
KroNI GCCGGC 2 cut(s) 90, 97
Kzo9I GATC 5 cut(s) 192, 207, 222, 325, 780
LmnI GCTCC 1 cut(s) 608
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 2 cut(s) 161, 664
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 857
MaeIII GTNAC 1 cut(s) 714
MalI GATC 5 cut(s) 194, 209, 224, 327, 782
MboI GATC 5 cut(s) 192, 207, 222, 325, 780
MboII GAAGA 8 cut(s) 122, 190, 217, 268, 634, 698, 790, 811
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 613
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 7 cut(s) 18, 250, 349, 460, 658, 724, 792
MluNI TGGCCA 1 cut(s) 548
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 1 cut(s) 703
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 5 cut(s) 379, 488, 513, 589, 730
Mox20I TGGCCA 1 cut(s) 548
MroNI GCCGGC 2 cut(s) 88, 95
MroXI GAANNNNTTC 1 cut(s) 114
MscI TGGCCA 1 cut(s) 548
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 4 cut(s) 89, 96, 789, 850
MspR9I CCNGG 4 cut(s) 103, 531, 606, 615
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 4 cut(s) 103, 531, 606, 615
MwoI GCNNNNNNNGC 7 cut(s) 53, 98, 138, 166, 194, 545, 859
NaeI GCCGGC 2 cut(s) 90, 97
NarI GGCGCC 1 cut(s) 99
NdeII GATC 5 cut(s) 192, 207, 222, 325, 780
NgoMIV GCCGGC 2 cut(s) 88, 95
NlaIII CATG 5 cut(s) 492, 622, 655, 742, 827
NlaIV GGNNCC 3 cut(s) 100, 610, 848
NspI RCATGY 2 cut(s) 492, 827
PaeI GCATGC 2 cut(s) 492, 827
PagI TCATGA 1 cut(s) 738
PctI GAATGC 1 cut(s) 362
PdiI GCCGGC 2 cut(s) 90, 97
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 526
PkrI GCNGC 4 cut(s) 94, 162, 668, 864
PleI GAGTC 1 cut(s) 703
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 703
Psp6I CCWGG 4 cut(s) 101, 529, 604, 613
PspGI CCWGG 4 cut(s) 101, 529, 604, 613
PspN4I GGNNCC 3 cut(s) 100, 610, 848
PsuI RGATCY 1 cut(s) 207
PvuII CAGCTG 1 cut(s) 160
RsaI GTAC 3 cut(s) 496, 629, 895
RsaNI GTAC 3 cut(s) 495, 628, 894
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 4 cut(s) 93, 161, 667, 863
Sau3AI GATC 5 cut(s) 192, 207, 222, 325, 780
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 4 cut(s) 103, 531, 606, 615
SduI GDGCHC 1 cut(s) 613
SfaNI GCATC 2 cut(s) 161, 664
SfoI GGCGCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 623
SphI GCATGC 2 cut(s) 492, 827
Sse9I AATT 7 cut(s) 18, 250, 349, 460, 658, 724, 792
SsiI CCGC 4 cut(s) 47, 92, 666, 862
SspDI GGCGCC 1 cut(s) 98
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 4 cut(s) 101, 529, 604, 613
StyI CCWWGG 1 cut(s) 445
TaiI ACGT 1 cut(s) 860
TaqI TCGA 3 cut(s) 174, 369, 722
TasI AATT 7 cut(s) 18, 250, 349, 460, 658, 724, 792
TatI WGTACW 1 cut(s) 627
TauI GCSGC 3 cut(s) 95, 669, 865
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 1 cut(s) 42
TseI GCWGC 1 cut(s) 160
TspDTI ATGAA 7 cut(s) 112, 441, 599, 635, 662, 668, 699
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 1 cut(s) 42
XapI RAATTY 2 cut(s) 18, 724
XceI RCATGY 2 cut(s) 492, 827
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.