MD02G1076900.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013965
Physical Location & Seq
Reverse (-)
6078891 .. 6079496
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1076900.v1.1.491

Sequence Viewer

Length: 606 bp
ATGACAACGTATGGGACTATCCCGACCGAACCCCCGGCATCCTCGAACCAACATTTCGTCGCACTCGCAAAAGAACAGATCCAATCAGCCCTAGGCAACCGAAGACCATGGCAGGAGATTATACAACTGCAAAATCTCACCCTTCCCTCCACCTTCAACCAATCAGTCCATCGAATCAAGACGAACGTCGTTTTCTTTCGCATGAACTACGCCATCATCACATTGTCCATCCTCTTCCTAAGCCTCCTATGGCACCCCATTTCGTTGATCGTCTTCATCGTCATGATGGTTGCATGGATCTTTCTATTTTTCCTGCATGACCAACCGTTGATGATTCTAGGGTACCGGATCAACGGGCAAGTGGTGATGATGGCCCTGTTGGTGGTCACACTCGTCGCGCTTTACCTTACTAATGCGAGATATCATATCATCGCGGGACTCTGCATTGGCCTTGCGGTTGTTCTGGTGCATGGAGCTCTGAGGGAGCCTGAGGATGTCTTCATTGTGGATGATGAAGGGCCTGGATCAGGTGGAGGTGGTGCGCATGTTCACAAAGTGCCTCTCAAACATGCTGCATCTTCTTCTTATTCTTTGGCCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

202

Amino Acids

22.3

Weight (kDa)

8.79

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 30 - 168 7.9e-43 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013965)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55190 AT3G13710 AT3G13720
malus_domestica MD02G1076900.v1.1 MD15G1205600.v1.1
prunus_persica Prupe.7G211500_v2.0.a1
pyrus_communis pycom02g06150 pycom15g18240
rosa_chinensis RchiOBHm_Chr2g0093141
rosa_laevigata RLG00000016372
rosa_roxburghii Rroxscaffold_2G00148470
rosa_rugosa Rorug02G0033200
rosa_samantha Rh2AG078800 Rh2BG080100 Rh2CG082300 Rh2DG077800
rosa_wichuraiana Rw2G006770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 543
Acc65I GGTACC 1 cut(s) 342
AccB1I GGYRCC 2 cut(s) 252, 342
AccII CGCG 2 cut(s) 398, 434
AciI CCGC 2 cut(s) 434, 455
AclWI GGATC 4 cut(s) 73, 305, 356, 532
AcoI YGGCCR 1 cut(s) 594
AdeI CACNNNGTG 1 cut(s) 556
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 2 cut(s) 382, 527
AgsI TTSAA 1 cut(s) 157
AjnI CCWGG 1 cut(s) 520
AluBI AGCT 1 cut(s) 476
AluI AGCT 1 cut(s) 476
Alw21I GWGCWC 1 cut(s) 478
AlwI GGATC 4 cut(s) 73, 305, 356, 532
AoxI GGCC 4 cut(s) 372, 448, 518, 594
ApeKI GCWGC 1 cut(s) 572
Asp718I GGTACC 1 cut(s) 342
AspA2I CCTAGG 1 cut(s) 91
AspLEI GCGC 2 cut(s) 400, 544
AspS9I GGNCC 2 cut(s) 373, 518
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 130, 376
AvrII CCTAGG 1 cut(s) 91
AxyI CCTNAGG 1 cut(s) 489
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 2 cut(s) 252, 342
BanII GRGCYC 1 cut(s) 478
BbsI GAAGAC 3 cut(s) 109, 265, 490
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 559
BccI CCATC 5 cut(s) 177, 221, 236, 280, 364
BciT130I CCWGG 1 cut(s) 522
BcnI CCSGG 1 cut(s) 35
BfaI CTAG 2 cut(s) 92, 338
BisI GCNGC 1 cut(s) 573
BlnI CCTAGG 1 cut(s) 91
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 2 cut(s) 35, 522
BmgT120I GGNCC 2 cut(s) 373, 518
BmiI GGNNCC 3 cut(s) 254, 344, 486
BmrFI CCNGG 2 cut(s) 35, 522
BmsI GCATC 2 cut(s) 47, 584
BoxI GACNNNNGTC 1 cut(s) 185
BpiI GAAGAC 3 cut(s) 109, 265, 490
Bpu10I CCTNAGC 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 33, 91, 107
BsaWI WCCGGW 1 cut(s) 345
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 527
Bse21I CCTNAGG 1 cut(s) 489
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 3 cut(s) 33, 91, 107
BseGI GGATG 4 cut(s) 38, 228, 499, 514
BseLI CCNNNNNNNGG 2 cut(s) 382, 527
BseMII CTCAG 2 cut(s) 470, 480
BseXI GCAGC 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 398, 434
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 4 cut(s) 374, 450, 520, 596
BshNI GGYRCC 2 cut(s) 252, 342
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 478
BsiSI CCGG 2 cut(s) 35, 346
BslFI GGGAC 2 cut(s) 28, 450
BslI CCNNNNNNNGG 2 cut(s) 382, 527
BsmFI GGGAC 2 cut(s) 28, 450
BsnI GGCC 4 cut(s) 374, 450, 520, 596
Bsp1286I GDGCHC 1 cut(s) 478
Bsp143I GATC 5 cut(s) 78, 267, 297, 348, 524
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 2 cut(s) 434, 455
BspANI GGCC 4 cut(s) 374, 450, 520, 596
BspCNI CTCAG 2 cut(s) 471, 481
BspFNI CGCG 2 cut(s) 398, 434
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 3 cut(s) 254, 344, 486
BspPI GGATC 4 cut(s) 73, 305, 356, 532
BspT107I GGYRCC 2 cut(s) 252, 342
BssECI CCNNGG 3 cut(s) 33, 91, 107
BssMI GATC 5 cut(s) 78, 267, 297, 348, 524
BssT1I CCWWGG 2 cut(s) 91, 107
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 1 cut(s) 327
Bst6I CTCTTC 1 cut(s) 239
BstDEI CTNAG 3 cut(s) 239, 479, 489
BstDSI CCRYGG 1 cut(s) 107
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 4 cut(s) 38, 228, 499, 514
BstFNI CGCG 2 cut(s) 398, 434
BstHHI GCGC 2 cut(s) 400, 544
BstKTI GATC 5 cut(s) 81, 270, 300, 351, 527
BstMBI GATC 5 cut(s) 78, 267, 297, 348, 524
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 2 cut(s) 548, 572
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 33, 520
BstUI CGCG 2 cut(s) 398, 434
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 3 cut(s) 109, 265, 490
BstX2I RGATCY 2 cut(s) 78, 297
BstYI RGATCY 2 cut(s) 78, 297
Bsu36I CCTNAGG 1 cut(s) 489
BsuRI GGCC 4 cut(s) 374, 450, 520, 596
BtgI CCRYGG 1 cut(s) 107
BtgZI GCGATG 1 cut(s) 415
BtsCI GGATG 4 cut(s) 38, 228, 499, 514
CciI TCATGA 1 cut(s) 282
CfoI GCGC 2 cut(s) 400, 544
Cfr13I GGNCC 2 cut(s) 373, 518
Csp6I GTAC 1 cut(s) 343
CviAII CATG 8 cut(s) 108, 202, 283, 294, 317, 470, 545, 569
CviJI RGCY 8 cut(s) 89, 243, 374, 450, 476, 487, 520, 596
CviKI_1 RGCY 8 cut(s) 89, 243, 374, 450, 476, 487, 520, 596
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 3 cut(s) 239, 479, 489
DpnI GATC 5 cut(s) 80, 269, 299, 350, 526
DpnII GATC 5 cut(s) 78, 267, 297, 348, 524
DraIII CACNNNGTG 1 cut(s) 556
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Ecl136II GAGCTC 1 cut(s) 476
Eco130I CCWWGG 2 cut(s) 91, 107
Eco24I GRGCYC 1 cut(s) 478
Eco32I GATATC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 476
Eco81I CCTNAGG 1 cut(s) 489
EcoICRI GAGCTC 1 cut(s) 476
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoO109I RGGNCCY 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 520
EcoRV GATATC 1 cut(s) 422
EcoT14I CCWWGG 2 cut(s) 91, 107
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 91, 107
FaeI CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 548, 572
FaqI GGGAC 2 cut(s) 28, 450
FatI CATG 8 cut(s) 107, 201, 282, 293, 316, 469, 544, 568
FauI CCCGC 1 cut(s) 427
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 4 cut(s) 25, 215, 506, 521
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 1 cut(s) 573
FspAI RTGCGCAY 1 cut(s) 543
FspBI CTAG 2 cut(s) 92, 338
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 2 cut(s) 399, 543
GluI GCNGC 1 cut(s) 573
HaeIII GGCC 4 cut(s) 374, 450, 520, 596
HapII CCGG 2 cut(s) 35, 346
HhaI GCGC 2 cut(s) 400, 544
Hin1II CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 548, 572
Hin6I GCGC 2 cut(s) 398, 542
HinP1I GCGC 2 cut(s) 398, 542
HinfI GANTC 3 cut(s) 174, 334, 438
HpaII CCGG 2 cut(s) 35, 346
HphI GGTGA 2 cut(s) 130, 376
Hpy166II GTNNAC 1 cut(s) 550
Hpy188I TCNGA 1 cut(s) 480
Hpy188III TCNNGA 3 cut(s) 22, 178, 283
Hpy8I GTNNAC 1 cut(s) 550
Hpy99I CGWCG 3 cut(s) 62, 191, 398
HpyAV CCTTC 3 cut(s) 152, 163, 509
HpyCH4III ACNGT 1 cut(s) 327
HpyCH4IV ACGT 2 cut(s) 8, 186
HpyCH4V TGCA 6 cut(s) 130, 293, 316, 444, 469, 575
HpyF3I CTNAG 3 cut(s) 239, 479, 489
HpySE526I ACGT 2 cut(s) 8, 186
Hsp92II CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 548, 572
HspAI GCGC 2 cut(s) 398, 542
KpnI GGTACC 1 cut(s) 346
Kzo9I GATC 5 cut(s) 78, 267, 297, 348, 524
LmnI GCTCC 2 cut(s) 473, 484
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 2 cut(s) 47, 584
MaeI CTAG 2 cut(s) 92, 338
MaeII ACGT 2 cut(s) 8, 186
MaeIII GTNAC 1 cut(s) 385
MalI GATC 5 cut(s) 80, 269, 299, 350, 526
MboI GATC 5 cut(s) 78, 267, 297, 348, 524
MboII GAAGA 6 cut(s) 114, 226, 265, 490, 570, 573
MflI RGATCY 2 cut(s) 78, 297
MhlI GDGCHC 1 cut(s) 478
MlsI TGGCCA 1 cut(s) 596
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 1 cut(s) 432
MnlI CCTC 8 cut(s) 52, 157, 242, 254, 474, 484, 527, 570
Mox20I TGGCCA 1 cut(s) 596
MscI TGGCCA 1 cut(s) 596
MslI CAYNNNNRTG 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 596
MspI CCGG 2 cut(s) 35, 346
MspR9I CCNGG 2 cut(s) 35, 522
MvaI CCWGG 1 cut(s) 522
MvnI CGCG 2 cut(s) 398, 434
NciI CCSGG 1 cut(s) 35
NcoI CCATGG 1 cut(s) 107
NdeII GATC 5 cut(s) 78, 267, 297, 348, 524
NlaIII CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 548, 572
NlaIV GGNNCC 3 cut(s) 254, 344, 486
NmuCI GTSAC 1 cut(s) 385
NsbI TGCGCA 1 cut(s) 543
NspI RCATGY 2 cut(s) 548, 572
PagI TCATGA 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PfeI GAWTC 2 cut(s) 174, 334
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
PshAI GACNNNNGTC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 3 cut(s) 254, 344, 486
PspPI GGNCC 2 cut(s) 373, 518
PsuI RGATCY 2 cut(s) 78, 297
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 281
SacI GAGCTC 1 cut(s) 478
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 5 cut(s) 78, 267, 297, 348, 524
Sau96I GGNCC 2 cut(s) 373, 518
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 2 cut(s) 35, 522
SduI GDGCHC 1 cut(s) 478
SetI ASST 7 cut(s) 11, 155, 189, 408, 478, 532, 538
SfaNI GCATC 2 cut(s) 47, 584
SmiMI CAYNNNNRTG 1 cut(s) 281
SsiI CCGC 2 cut(s) 434, 455
SspMI CTAG 2 cut(s) 92, 338
SstI GAGCTC 1 cut(s) 478
StyD4I CCNGG 2 cut(s) 33, 520
StyI CCWWGG 2 cut(s) 91, 107
TaaI ACNGT 1 cut(s) 327
TaiI ACGT 2 cut(s) 11, 189
TaqI TCGA 2 cut(s) 44, 172
TaqII GACCGA 1 cut(s) 41
TfiI GAWTC 2 cut(s) 174, 334
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 4 cut(s) 218, 265, 490, 528
XagI CCTNNNNNAGG 1 cut(s) 525
XceI RCATGY 2 cut(s) 548, 572
XmaJI CCTAGG 1 cut(s) 91
XspI CTAG 2 cut(s) 92, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.