Rroxscaffold_2G00148470

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013965
Physical Location & Seq
Forward (+)
86160970 .. 86164868
3899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148470.1

Sequence Viewer

Length: 600 bp
ATGACGACCTACGGAACTATCCCGACAGAGCGTTTGCCATCCTCGAACCTAGATATCGTTTCACAAGCAAAAGGACAGATTGAATTATGCCTTGGCATACAAAGACCATGGCTGGAGATGATTCAGCTCCAAGATCTCAAACCTCCCTCCTCCTTCAACCAATCAATCCAAAGAATCAGGACAAACGTAGCCTTCTTCCGCATGAACTACGCCATTATCATATTGACCATTCTCTTCATAAGCCTTCTATGGTACCCCATCTCATTGATGATCTTCATCGTCACGATGGCTGCATGGTTGTTTCTATATTATTTACATGACAAACCCTTGGTGGTTTTAGGCTATGAAATTGATCAACGATTCCTGATGGCAGCTTTGTTCTTGGTCACAGCCCTGATACTTTCCCTTACCAGTGTGAAATATAACATTATAATGGCACTCTCTATTGGCCTTGCTTTTGTTTTGGTTCATGGAGCCTTAAGGGAGCCAGAGGATGTCTTGACTGTGGATGATGAAGAAGGGCTTGCATCAGGTGGTAGCCCTGTTCTAAAAGTCCCTCTGAAAAATGCTGCCTCTTCTTCTTATTCTCTGTCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

199

Amino Acids

22.27

Weight (kDa)

5.64

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 34 - 166 2.2e-40 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013965)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55190 AT3G13710 AT3G13720
malus_domestica MD02G1076900.v1.1 MD15G1205600.v1.1
prunus_persica Prupe.7G211500_v2.0.a1
pyrus_communis pycom02g06150 pycom15g18240
rosa_chinensis RchiOBHm_Chr2g0093141
rosa_laevigata RLG00000016372
rosa_roxburghii Rroxscaffold_2G00148470
rosa_rugosa Rorug02G0033200
rosa_samantha Rh2AG078800 Rh2BG080100 Rh2CG082300 Rh2DG077800
rosa_wichuraiana Rw2G006770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 252
AciI CCGC 1 cut(s) 199
AfaI GTAC 1 cut(s) 254
AflII CTTAAG 1 cut(s) 478
AgsI TTSAA 2 cut(s) 83, 157
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AluBI AGCT 2 cut(s) 127, 374
AluI AGCT 2 cut(s) 127, 374
AoxI GGCC 1 cut(s) 448
ApeKI GCWGC 3 cut(s) 290, 371, 569
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
Asp718I GGTACC 1 cut(s) 252
BanI GGYRCC 1 cut(s) 252
BbvI GCAGC 3 cut(s) 277, 383, 556
BccI CCATC 4 cut(s) 46, 266, 280, 361
BclI TGATCA 1 cut(s) 352
BfaI CTAG 1 cut(s) 50
BfrI CTTAAG 1 cut(s) 478
BglII AGATCT 1 cut(s) 133
BisI GCNGC 3 cut(s) 291, 372, 570
BlsI GCNGC 3 cut(s) 292, 373, 571
BmiI GGNNCC 3 cut(s) 254, 475, 486
BmsI GCATC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 134
BsaBI GATNNNNATC 1 cut(s) 275
BsaJI CCNNGG 3 cut(s) 91, 107, 327
Bse1I ACTGG 1 cut(s) 411
Bse8I GATNNNNATC 1 cut(s) 275
BseDI CCNNGG 3 cut(s) 91, 107, 327
BseGI GGATG 3 cut(s) 38, 499, 514
BseJI GATNNNNATC 1 cut(s) 275
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 277, 383, 556
BshFI GGCC 1 cut(s) 450
BshNI GGYRCC 1 cut(s) 252
BslFI GGGAC 2 cut(s) 539, 577
BsmFI GGGAC 2 cut(s) 539, 577
BsnI GGCC 1 cut(s) 450
Bsp143I GATC 3 cut(s) 133, 270, 352
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 1 cut(s) 199
BspANI GGCC 1 cut(s) 450
BspLI GGNNCC 3 cut(s) 254, 475, 486
BspT107I GGYRCC 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 478
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 3 cut(s) 91, 107, 327
BssMI GATC 3 cut(s) 133, 270, 352
BssT1I CCWWGG 3 cut(s) 91, 107, 327
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 2 cut(s) 239, 580
BstAFI CTTAAG 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 525
BstDSI CCRYGG 1 cut(s) 107
BstF5I GGATG 3 cut(s) 38, 499, 514
BstKTI GATC 3 cut(s) 136, 273, 355
BstMBI GATC 3 cut(s) 133, 270, 352
BstV1I GCAGC 3 cut(s) 277, 383, 556
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 107
BtsCI GGATG 3 cut(s) 38, 499, 514
BtsIMutI CAGTG 1 cut(s) 418
Cac8I GCNNGC 1 cut(s) 525
Csp6I GTAC 1 cut(s) 253
CviAII CATG 5 cut(s) 108, 202, 294, 317, 470
CviQI GTAC 1 cut(s) 253
DpnI GATC 3 cut(s) 135, 272, 354
DpnII GATC 3 cut(s) 133, 270, 352
Eam1104I CTCTTC 2 cut(s) 239, 580
EarI CTCTTC 2 cut(s) 239, 580
Eco130I CCWWGG 3 cut(s) 91, 107, 327
Eco32I GATATC 1 cut(s) 55
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 3 cut(s) 91, 107, 327
ErhI CCWWGG 3 cut(s) 91, 107, 327
FaeI CATG 5 cut(s) 111, 205, 297, 320, 473
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 2 cut(s) 539, 577
FatI CATG 5 cut(s) 107, 201, 293, 316, 469
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 291, 372, 570
FokI GGATG 3 cut(s) 25, 506, 521
Fsp4HI GCNGC 3 cut(s) 291, 372, 570
FspBI CTAG 1 cut(s) 50
GluI GCNGC 3 cut(s) 291, 372, 570
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 1 cut(s) 450
Hin1II CATG 5 cut(s) 111, 205, 297, 320, 473
HinfI GANTC 3 cut(s) 121, 174, 360
Hpy188I TCNGA 1 cut(s) 561
Hpy188III TCNNGA 5 cut(s) 22, 178, 283, 364, 499
HpyAV CCTTC 4 cut(s) 163, 202, 254, 512
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 293, 527
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 5 cut(s) 111, 205, 297, 320, 473
KpnI GGTACC 1 cut(s) 256
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 3 cut(s) 133, 270, 352
LmnI GCTCC 3 cut(s) 132, 473, 484
LpnPI CCDG 8 cut(s) 98, 163, 377, 407, 424, 501, 516, 555
Lsp1109I GCAGC 3 cut(s) 277, 383, 556
LweI GCATC 1 cut(s) 536
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 280, 385
MalI GATC 3 cut(s) 135, 272, 354
MboI GATC 3 cut(s) 133, 270, 352
MboII GAAGA 6 cut(s) 187, 226, 265, 527, 567, 570
MflI RGATCY 1 cut(s) 133
MluCI AATT 2 cut(s) 83, 348
MnlI CCTC 7 cut(s) 52, 153, 157, 160, 484, 567, 583
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 478
NcoI CCATGG 1 cut(s) 107
NdeII GATC 3 cut(s) 133, 270, 352
NlaIII CATG 5 cut(s) 111, 205, 297, 320, 473
NlaIV GGNNCC 3 cut(s) 254, 475, 486
NmuCI GTSAC 2 cut(s) 280, 385
PfeI GAWTC 3 cut(s) 121, 174, 360
PkrI GCNGC 3 cut(s) 292, 373, 571
PsiI TTATAA 1 cut(s) 431
PspN4I GGNNCC 3 cut(s) 254, 475, 486
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 3 cut(s) 291, 372, 570
Sau3AI GATC 3 cut(s) 133, 270, 352
SetI ASST 7 cut(s) 11, 51, 129, 145, 189, 376, 535
SfaNI GCATC 1 cut(s) 536
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse9I AATT 2 cut(s) 83, 348
SsiI CCGC 1 cut(s) 199
SspMI CTAG 1 cut(s) 50
StyI CCWWGG 3 cut(s) 91, 107, 327
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 44
TasI AATT 2 cut(s) 83, 348
TfiI GAWTC 3 cut(s) 121, 174, 360
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 2 cut(s) 280, 385
TseI GCWGC 3 cut(s) 290, 371, 569
Tsp45I GTSAC 2 cut(s) 280, 385
TspDTI ATGAA 6 cut(s) 218, 226, 265, 360, 458, 528
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 418
Vha464I CTTAAG 1 cut(s) 478
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.