MD15G1205600.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013965
Physical Location & Seq
Reverse (-)
16337357 .. 16337962
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1205600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGACAACGTACGGGACTATCACGACCGAACCCCTGCCATCATCGAACCAACATTTCGTCACACGCGCAAAAGAACAGATCCAGACAGGCCTAGAGAACCGAAGACCATGGCTGGAGATGATACAACTGCAAAATCTCACCCTTCCCTCCACCTTCAACCAATCAATCCACAGAATCAAGACGAACGTCGCCTTCTATCGCATGAACTATGCCATCATCACATTGTTCATCCTCTTCCTGAGCCTCCTATGGCACCCTATTTCGTTGATCGTCTTCGTTGTCATGATGGTTGCATGGTTCTTTCTATTTTTCCTGCATGACCAACCCTTGATGATTCTAGGGTACCGAATCAACGAACGAGTGGTGACGATGGCTCTGTTGGTGGTCACAGTCGTCGCACTTTTCCTTACCAATGCGAGATATCATATCGTCGCCGGACTCTCCATTGGCCTCACGGTTGTTCTGGTGCATGGAGCTCTGAGGGTGCCTGAGGATGTCTTCATTGTGGATGATGAAGAAGGGCTTGGATCAGGTGAAGGTGGTGCACATGTTCTCAAACTGCCTCTCAAACATGCTGCCTCTTCTTCTTATTCTTTGGCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

202

Amino Acids

22.67

Weight (kDa)

8.1

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 31 - 168 5.7e-43 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013965)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55190 AT3G13710 AT3G13720
malus_domestica MD02G1076900.v1.1 MD15G1205600.v1.1
prunus_persica Prupe.7G211500_v2.0.a1
pyrus_communis pycom02g06150 pycom15g18240
rosa_chinensis RchiOBHm_Chr2g0093141
rosa_laevigata RLG00000016372
rosa_roxburghii Rroxscaffold_2G00148470
rosa_rugosa Rorug02G0033200
rosa_samantha Rh2AG078800 Rh2BG080100 Rh2CG082300 Rh2DG077800
rosa_wichuraiana Rw2G006770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 342
AccB1I GGYRCC 3 cut(s) 252, 342, 484
AccII CGCG 1 cut(s) 66
AclWI GGATC 2 cut(s) 73, 535
AcoI YGGCCR 1 cut(s) 597
AfaI GTAC 2 cut(s) 11, 344
AflIII ACRYGT 1 cut(s) 547
AgsI TTSAA 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 507, 539
AluBI AGCT 1 cut(s) 476
AluI AGCT 1 cut(s) 476
Alw21I GWGCWC 2 cut(s) 478, 547
Alw44I GTGCAC 1 cut(s) 543
AlwI GGATC 2 cut(s) 73, 535
AoxI GGCC 3 cut(s) 88, 448, 597
ApaLI GTGCAC 1 cut(s) 543
ApeKI GCWGC 1 cut(s) 575
Asp718I GGTACC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 68
AsuHPI GGTGA 3 cut(s) 130, 376, 545
AxyI CCTNAGG 1 cut(s) 489
BaeGI GKGCMC 1 cut(s) 547
BalI TGGCCA 1 cut(s) 599
BanI GGYRCC 3 cut(s) 252, 342, 484
BanII GRGCYC 1 cut(s) 478
BbsI GAAGAC 3 cut(s) 109, 265, 490
Bbv12I GWGCWC 2 cut(s) 478, 547
BbvI GCAGC 1 cut(s) 562
BccI CCATC 4 cut(s) 46, 221, 280, 364
BfaI CTAG 2 cut(s) 92, 338
BisI GCNGC 1 cut(s) 576
BlsI GCNGC 1 cut(s) 577
BmiI GGNNCC 3 cut(s) 254, 344, 486
BoxI GACNNNNGTC 1 cut(s) 185
BpiI GAAGAC 3 cut(s) 109, 265, 490
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 107
Bse21I CCTNAGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 107
BseGI GGATG 3 cut(s) 228, 499, 514
BseMII CTCAG 3 cut(s) 230, 470, 480
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 1 cut(s) 562
Bsh1236I CGCG 1 cut(s) 66
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 3 cut(s) 90, 450, 599
BshNI GGYRCC 3 cut(s) 252, 342, 484
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 478, 547
BsiSI CCGG 1 cut(s) 435
BsiWI CGTACG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 3 cut(s) 90, 450, 599
Bsp1286I GDGCHC 2 cut(s) 478, 547
Bsp143I GATC 3 cut(s) 78, 267, 527
Bsp19I CCATGG 1 cut(s) 107
BspANI GGCC 3 cut(s) 90, 450, 599
BspCNI CTCAG 3 cut(s) 231, 471, 481
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 3 cut(s) 254, 344, 486
BspPI GGATC 2 cut(s) 73, 535
BspT107I GGYRCC 3 cut(s) 252, 342, 484
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 3 cut(s) 78, 267, 527
BssT1I CCWWGG 1 cut(s) 107
Bst4CI ACNGT 2 cut(s) 391, 457
Bst6I CTCTTC 2 cut(s) 239, 586
BstDEI CTNAG 3 cut(s) 239, 479, 489
BstDSI CCRYGG 1 cut(s) 107
BstF5I GGATG 3 cut(s) 228, 499, 514
BstFNI CGCG 1 cut(s) 66
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 3 cut(s) 81, 270, 530
BstMBI GATC 3 cut(s) 78, 267, 527
BstMCI CGRYCG 1 cut(s) 27
BstNSI RCATGY 2 cut(s) 551, 575
BstPAI GACNNNNGTC 1 cut(s) 185
BstSLI GKGCMC 1 cut(s) 547
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 3 cut(s) 109, 265, 490
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu36I CCTNAGG 1 cut(s) 489
BsuRI GGCC 3 cut(s) 90, 450, 599
BtgI CCRYGG 1 cut(s) 107
BtsCI GGATG 3 cut(s) 228, 499, 514
CciI TCATGA 1 cut(s) 282
CfoI GCGC 1 cut(s) 68
Csp6I GTAC 2 cut(s) 10, 343
CviAII CATG 8 cut(s) 108, 202, 283, 294, 317, 470, 548, 572
CviJI RGCY 8 cut(s) 90, 112, 243, 374, 450, 476, 523, 599
CviKI_1 RGCY 8 cut(s) 90, 112, 243, 374, 450, 476, 523, 599
CviQI GTAC 2 cut(s) 10, 343
DdeI CTNAG 3 cut(s) 239, 479, 489
DpnI GATC 3 cut(s) 80, 269, 529
DpnII GATC 3 cut(s) 78, 267, 527
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 2 cut(s) 239, 586
EarI CTCTTC 2 cut(s) 239, 586
Ecl136II GAGCTC 1 cut(s) 476
Eco130I CCWWGG 1 cut(s) 107
Eco147I AGGCCT 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 478
Eco32I GATATC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 476
Eco81I CCTNAGG 1 cut(s) 489
EcoICRI GAGCTC 1 cut(s) 476
EcoRV GATATC 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 551, 575
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 1 cut(s) 28
FatI CATG 8 cut(s) 107, 201, 282, 293, 316, 469, 547, 571
Fnu4HI GCNGC 1 cut(s) 576
FokI GGATG 3 cut(s) 215, 506, 521
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 2 cut(s) 92, 338
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 576
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 3 cut(s) 90, 450, 599
HapII CCGG 1 cut(s) 435
HhaI GCGC 1 cut(s) 68
Hin1II CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 551, 575
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HinfI GANTC 4 cut(s) 174, 334, 348, 438
HpaII CCGG 1 cut(s) 435
HphI GGTGA 3 cut(s) 130, 376, 545
Hpy166II GTNNAC 1 cut(s) 545
Hpy188I TCNGA 1 cut(s) 480
Hpy188III TCNNGA 5 cut(s) 22, 82, 178, 238, 283
Hpy8I GTNNAC 1 cut(s) 545
Hpy99I CGWCG 3 cut(s) 191, 398, 434
HpyAV CCTTC 5 cut(s) 152, 163, 202, 512, 530
HpyCH4III ACNGT 2 cut(s) 391, 457
HpyCH4IV ACGT 2 cut(s) 8, 186
HpyCH4V TGCA 5 cut(s) 130, 293, 316, 469, 545
HpyF3I CTNAG 3 cut(s) 239, 479, 489
HpySE526I ACGT 2 cut(s) 8, 186
Hsp92II CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 551, 575
HspAI GCGC 1 cut(s) 66
KpnI GGTACC 1 cut(s) 346
Kzo9I GATC 3 cut(s) 78, 267, 527
LmnI GCTCC 1 cut(s) 473
Lsp1109I GCAGC 1 cut(s) 562
MaeI CTAG 2 cut(s) 92, 338
MaeII ACGT 2 cut(s) 8, 186
MaeIII GTNAC 3 cut(s) 58, 364, 385
MalI GATC 3 cut(s) 80, 269, 529
MboI GATC 3 cut(s) 78, 267, 527
MboII GAAGA 7 cut(s) 114, 226, 265, 490, 527, 573, 576
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 2 cut(s) 478, 547
MlsI TGGCCA 1 cut(s) 599
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 1 cut(s) 432
MnlI CCTC 8 cut(s) 157, 242, 254, 461, 474, 484, 573, 589
Mox20I TGGCCA 1 cut(s) 599
MscI TGGCCA 1 cut(s) 599
Msp20I TGGCCA 1 cut(s) 599
MspI CCGG 1 cut(s) 435
MvnI CGCG 1 cut(s) 66
NcoI CCATGG 1 cut(s) 107
NdeII GATC 3 cut(s) 78, 267, 527
NlaIII CATG 8 cut(s) 111, 205, 286, 297, 320, 473, 551, 575
NlaIV GGNNCC 3 cut(s) 254, 344, 486
NmuCI GTSAC 3 cut(s) 58, 364, 385
NspI RCATGY 2 cut(s) 551, 575
PagI TCATGA 1 cut(s) 282
PceI AGGCCT 1 cut(s) 90
PciI ACATGT 1 cut(s) 547
PfeI GAWTC 3 cut(s) 174, 334, 348
Pfl23II CGTACG 1 cut(s) 9
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
PscI ACATGT 1 cut(s) 547
PshAI GACNNNNGTC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 478
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 254, 344, 486
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 2 cut(s) 11, 344
RsaNI GTAC 2 cut(s) 10, 343
SacI GAGCTC 1 cut(s) 478
SatI GCNGC 1 cut(s) 576
Sau3AI GATC 3 cut(s) 78, 267, 527
SchI GAGTC 1 cut(s) 432
SduI GDGCHC 2 cut(s) 478, 547
SetI ASST 6 cut(s) 11, 155, 189, 478, 535, 541
SseBI AGGCCT 1 cut(s) 90
SspMI CTAG 2 cut(s) 92, 338
SstI GAGCTC 1 cut(s) 478
StuI AGGCCT 1 cut(s) 90
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 2 cut(s) 391, 457
TaiI ACGT 2 cut(s) 11, 189
TaqI TCGA 1 cut(s) 44
TaqII GACCGA 1 cut(s) 41
TfiI GAWTC 3 cut(s) 174, 334, 348
TseFI GTSAC 3 cut(s) 58, 364, 385
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 3 cut(s) 58, 364, 385
TspDTI ATGAA 4 cut(s) 217, 218, 490, 528
VneI GTGCAC 1 cut(s) 543
XceI RCATGY 2 cut(s) 551, 575
XspI CTAG 2 cut(s) 92, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.