MD02G1086200.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011508
Physical Location & Seq
Reverse (-)
6760401 .. 6763923
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1086200.v1.1.491

Sequence Viewer

Length: 927 bp
ATGCCCCTTTATACAAATTCCATGTCGTGGAATCCAAGTGAACAACAAGTTCCACAACCTTTATCCAAAGATGTAAGCTTCAAAGTGAAATTTCCGCCACAGGTTCATCATGGCTCAAAGCATTTAGGACTCCAACTACAAACAGACCAGGGATCATCATCCATAACCCAATCAATCGGTCACTCTCAAGATCAATGCAACTCATCAGATTCTGGCGAAGTTCAAACATGTGGTAAGGATGCTGAAGGCAACATGAAGCCAGTTTTCTTGCAGAGTAATCAAGATTTCATGCTGAATCCTTCTCAAGTTAGTCACGGAAATTTAATGGGTTGCGTGCCGTATCCTTATGTCGACCCTTACTTCAGCGGGTTCTTGACTGCATATGGACCACAGGCGATGTCCCATATGCTTGGGATGGCACCTACACGAGTTCCATTGCCTCTCGATCTGGCACAAGACGGACCCATCTATGTCAATGCAAAACAATACCACGGAATCCTCAGAAGGAGACAGTCCCGTGCAAAGTTGGAGGCTCGTAACAAACTTCTCAAAGCTCGAAAGCCTTATCTCCATGAGTCTCGCCATCTTCATGCCGTGAATAGGGTAAGGGGCACTGGTGGACGTTTTCTCAGCACAAAACGGCAGTCAGACCAGAACCCCTCCAGTATCACCCACCATGTTTCGGACTCCATCAGCTCACATCAGAAAGATACAGGGGGCACAGAAAGTAATCACTCGGGAAGCAGTGATTTCCTCCCTAGTGTTGCACTCCACCCAGACATGACTAGCGTTTCCAACACCAATGACAACTTTCAGCAACCAGATCGCAGATTCTCAGGCATCCCTCCCCACATGAGTGGAGCAATGCAGTTCCCTGGCGGGTTTATGCGTGCTGGAAACCAGCACTGTGCTTCTGTTGTCCGGTGA

Protein Analysis

309

Amino Acids

33.93

Weight (kDa)

9.28

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 155 - 209 2.7e-27 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 27
AccI GTMKAC 1 cut(s) 351
AciI CCGC 3 cut(s) 95, 366, 879
AclWI GGATC 1 cut(s) 160
AcsI RAATTY 3 cut(s) 16, 89, 319
AcuI CTGAAG 2 cut(s) 264, 346
AfiI CCNNNNNNNGG 3 cut(s) 27, 600, 682
AflIII ACRYGT 1 cut(s) 227
AgsI TTSAA 2 cut(s) 82, 224
AjnI CCWGG 2 cut(s) 147, 874
AleI CACNNNNGTG 1 cut(s) 855
AluBI AGCT 3 cut(s) 78, 554, 696
AluI AGCT 3 cut(s) 78, 554, 696
Alw26I GTCTC 2 cut(s) 502, 582
AlwI GGATC 1 cut(s) 160
AlwNI CAGNNNCTG 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 736
ApoI RAATTY 3 cut(s) 16, 89, 319
ArsI GACNNNNNNTTYG 4 cut(s) 8, 40, 629, 661
AspS9I GGNCC 2 cut(s) 386, 461
AsuHPI GGTGA 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 736
AvaII GGWCC 2 cut(s) 386, 461
BaeGI GKGCMC 2 cut(s) 614, 722
BanI GGYRCC 1 cut(s) 418
BauI CACGAG 1 cut(s) 426
BccI CCATC 4 cut(s) 409, 473, 591, 698
BceAI ACGGC 3 cut(s) 322, 578, 656
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BciT130I CCWGG 2 cut(s) 149, 876
BciVI GTATCC 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 502, 582
BfaI CTAG 2 cut(s) 759, 786
BfuI GTATCC 1 cut(s) 351
Bme1390I CCNGG 2 cut(s) 149, 876
Bme18I GGWCC 2 cut(s) 386, 461
BmeT110I CYCGRG 1 cut(s) 736
BmgT120I GGNCC 2 cut(s) 386, 461
BmiI GGNNCC 2 cut(s) 420, 463
BmrFI CCNGG 2 cut(s) 149, 876
BmsI GCATC 2 cut(s) 229, 849
BpmI CTGGAG 1 cut(s) 646
BpuEI CTTGAG 2 cut(s) 171, 288
BsaBI GATNNNNATC 1 cut(s) 157
BsaJI CCNNGG 3 cut(s) 148, 490, 874
BsaWI WCCGGW 1 cut(s) 921
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 600, 682
Bse1I ACTGG 3 cut(s) 260, 619, 663
Bse3DI GCAATG 2 cut(s) 434, 870
Bse8I GATNNNNATC 1 cut(s) 157
BseBI CCWGG 2 cut(s) 149, 876
BseDI CCNNGG 3 cut(s) 148, 490, 874
BseGI GGATG 4 cut(s) 158, 244, 420, 840
BseJI GATNNNNATC 1 cut(s) 157
BseLI CCNNNNNNNGG 3 cut(s) 27, 600, 682
BseMI GCAATG 2 cut(s) 434, 870
BseMII CTCAG 3 cut(s) 514, 643, 849
BseNI ACTGG 3 cut(s) 260, 619, 663
BseSI GKGCMC 2 cut(s) 614, 722
BshNI GGYRCC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 736
BsiSI CCGG 1 cut(s) 922
BslFI GGGAC 2 cut(s) 385, 499
BslI CCNNNNNNNGG 3 cut(s) 27, 600, 682
BsmAI GTCTC 2 cut(s) 502, 582
BsmFI GGGAC 2 cut(s) 385, 499
BsoBI CYCGRG 1 cut(s) 736
Bsp1286I GDGCHC 2 cut(s) 614, 722
Bsp143I GATC 4 cut(s) 152, 190, 445, 823
BspACI CCGC 3 cut(s) 95, 366, 879
BspCNI CTCAG 3 cut(s) 513, 642, 848
BspLI GGNNCC 2 cut(s) 420, 463
BspPI GGATC 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 418
BsrDI GCAATG 2 cut(s) 434, 870
BsrI ACTGG 3 cut(s) 260, 619, 663
BssECI CCNNGG 3 cut(s) 148, 490, 874
BssMI GATC 4 cut(s) 152, 190, 445, 823
BssSI CACGAG 1 cut(s) 426
Bst2BI CACGAG 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 149, 876
Bst4CI ACNGT 2 cut(s) 513, 908
BstC8I GCNNGC 2 cut(s) 335, 891
BstDEI CTNAG 3 cut(s) 500, 629, 835
BstDSI CCRYGG 1 cut(s) 490
BstF5I GGATG 4 cut(s) 158, 244, 420, 840
BstKTI GATC 4 cut(s) 155, 193, 448, 826
BstMAI GTCTC 2 cut(s) 502, 582
BstMBI GATC 4 cut(s) 152, 190, 445, 823
BstNI CCWGG 2 cut(s) 149, 876
BstNSI RCATGY 1 cut(s) 231
BstSCI CCNGG 2 cut(s) 147, 874
BstSLI GKGCMC 2 cut(s) 614, 722
BstXI CCANNNNNNTGG 2 cut(s) 410, 857
BsuI GTATCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 490
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 4 cut(s) 158, 244, 420, 840
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 3 cut(s) 612, 751, 904
Cac8I GCNNGC 2 cut(s) 335, 891
CaiI CAGNNNCTG 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 386, 461
CviJI RGCY 7 cut(s) 78, 114, 259, 533, 554, 562, 696
CviKI_1 RGCY 7 cut(s) 78, 114, 259, 533, 554, 562, 696
DdeI CTNAG 3 cut(s) 500, 629, 835
DpnI GATC 4 cut(s) 154, 192, 447, 825
DpnII GATC 4 cut(s) 152, 190, 445, 823
EciI GGCGGA 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 386, 461
Eco57I CTGAAG 2 cut(s) 264, 346
Eco88I CYCGRG 1 cut(s) 736
EcoRII CCWGG 2 cut(s) 147, 874
FaqI GGGAC 2 cut(s) 385, 499
FauI CCCGC 2 cut(s) 359, 872
FauNDI CATATG 2 cut(s) 382, 405
FblI GTMKAC 1 cut(s) 351
FokI GGATG 4 cut(s) 145, 251, 427, 827
FspBI CTAG 2 cut(s) 759, 786
GsuI CTGGAG 1 cut(s) 646
HapII CCGG 1 cut(s) 922
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 8 cut(s) 31, 129, 209, 295, 495, 575, 686, 831
HpaII CCGG 1 cut(s) 922
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 3 cut(s) 41, 352, 620
Hpy188I TCNGA 5 cut(s) 208, 503, 649, 685, 705
Hpy188III TCNNGA 5 cut(s) 188, 281, 373, 443, 738
Hpy8I GTNNAC 3 cut(s) 41, 352, 620
HpyAV CCTTC 3 cut(s) 239, 309, 498
HpyCH4III ACNGT 2 cut(s) 513, 908
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 7 cut(s) 198, 271, 380, 479, 521, 767, 868
HpyF3I CTNAG 3 cut(s) 500, 629, 835
HpySE526I ACGT 1 cut(s) 622
Kzo9I GATC 4 cut(s) 152, 190, 445, 823
LmnI GCTCC 1 cut(s) 860
LweI GCATC 2 cut(s) 229, 849
MaeI CTAG 2 cut(s) 759, 786
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 3 cut(s) 179, 311, 536
MalI GATC 4 cut(s) 154, 192, 447, 825
MboI GATC 4 cut(s) 152, 190, 445, 823
MboII GAAGA 1 cut(s) 578
MhlI GDGCHC 2 cut(s) 614, 722
MluCI AATT 3 cut(s) 16, 89, 319
MlyI GAGTC 3 cut(s) 123, 584, 680
MmeI TCCRAC 3 cut(s) 157, 507, 819
MnlI CCTC 6 cut(s) 450, 509, 523, 670, 764, 855
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 2 cut(s) 588, 855
MspA1I CMGCKG 1 cut(s) 366
MspI CCGG 1 cut(s) 922
MspR9I CCNGG 2 cut(s) 149, 876
MvaI CCWGG 2 cut(s) 149, 876
NdeI CATATG 2 cut(s) 382, 405
NdeII GATC 4 cut(s) 152, 190, 445, 823
NlaIV GGNNCC 2 cut(s) 420, 463
NmuCI GTSAC 2 cut(s) 179, 311
NspI RCATGY 1 cut(s) 231
OliI CACNNNNGTG 1 cut(s) 855
PciI ACATGT 1 cut(s) 227
PfeI GAWTC 5 cut(s) 31, 209, 295, 495, 831
PflMI CCANNNNNTGG 1 cut(s) 27
PleI GAGTC 3 cut(s) 123, 583, 680
PpsI GAGTC 3 cut(s) 123, 583, 680
PscI ACATGT 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 147, 874
PspGI CCWGG 2 cut(s) 147, 874
PspN4I GGNNCC 2 cut(s) 420, 463
PspPI GGNCC 2 cut(s) 386, 461
PstNI CAGNNNCTG 1 cut(s) 212
RseI CAYNNNNRTG 2 cut(s) 588, 855
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 323
Sau3AI GATC 4 cut(s) 152, 190, 445, 823
Sau96I GGNCC 2 cut(s) 386, 461
SchI GAGTC 3 cut(s) 123, 584, 680
ScrFI CCNGG 2 cut(s) 149, 876
SduI GDGCHC 2 cut(s) 614, 722
SetI ASST 7 cut(s) 61, 80, 105, 424, 556, 625, 698
SfaNI GCATC 2 cut(s) 229, 849
SinI GGWCC 2 cut(s) 386, 461
SmiMI CAYNNNNRTG 2 cut(s) 588, 855
SmlI CTYRAG 2 cut(s) 186, 303
SmoI CTYRAG 2 cut(s) 186, 303
Sse9I AATT 3 cut(s) 16, 89, 319
SsiI CCGC 3 cut(s) 95, 366, 879
SspMI CTAG 2 cut(s) 759, 786
StyD4I CCNGG 2 cut(s) 147, 874
TaaI ACNGT 2 cut(s) 513, 908
TaiI ACGT 1 cut(s) 625
TaqI TCGA 3 cut(s) 351, 444, 556
TaqII GACCGA 1 cut(s) 167
TasI AATT 3 cut(s) 16, 89, 319
TfiI GAWTC 5 cut(s) 31, 209, 295, 495, 831
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 3 cut(s) 619, 751, 911
TseFI GTSAC 2 cut(s) 179, 311
Tsp45I GTSAC 2 cut(s) 179, 311
TspDTI ATGAA 4 cut(s) 95, 269, 277, 578
TspGWI ACGGA 3 cut(s) 330, 474, 507
TspRI CASTG 3 cut(s) 619, 751, 911
Van91I CCANNNNNTGG 1 cut(s) 27
VpaK11BI GGWCC 2 cut(s) 386, 461
XapI RAATTY 3 cut(s) 16, 89, 319
XceI RCATGY 1 cut(s) 231
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 2 cut(s) 759, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.