MD15G1213400.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011508
Physical Location & Seq
Reverse (-)
17122823 .. 17125801
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1213400.v1.1.491

Sequence Viewer

Length: 903 bp
ATGCCCCTTTATACTAATTCCATGTCGTGGATTCCAAGTGAACGACAAATTTCAGAGTCTTTATCCAAGGATGTGAGCTTCAAAGTGGTATTTCCGCCACAAGGTTATCATGACGCAAAGCATTTAGGACTTAAACTACAAACAGACCAGGGATCATCATCCACAACCCACTCAATTGTTCACTCTCAAGGCGAAGATGAAACATGTGGTAAGGGTGCTGATGGGAAACTGAAGCCAGTTTTCTTGCCGAGTAATCAAGATTTAATGTCGAACCCTTCTCAAGTTGGTTACGGCAATTTAGTGGGCTGCATACCATATCCTTATGTTGACCCTTACTTCAGTGGGTTCATGACTGCATATGGATCACAGGCTATGTCCCATATGCTTGGGATCGTACCTACACGAGTTCTGTTGCCTCCTGATCTGGCACAAGATGGACCCATCTATGTCAATGCAAAACAGTACCGTGGAATCCTCAGAAGGAGACAGTCCCGTGCAAAGTTGGAGGCTCAAAACAAACTTCTCAAAGCTCGAAAGCCTTATCTCCACGAGTCTCGCCATCTTCATGCCCTGAATAGGGTAAGGGGCTCTGGTGGACGTTTTCTCAGCACAAAGCGGCAATCAGACCAGAACGCCTCCAGTAGTACCCACCACGTTTCTGACTCCATCAGCGTACTTCAGAAAGATACAGGGGACACAGAAAGTCATCACTCGGAAAGCAGTGAATTCCTCCCTAGTGTTGCAATTCACTCAGACATGACAAGTGTTTCCAACACAAATGACAACTTTCGGCAGCCAGATCGCAGGTTTGTAGGCATCCCTCCCCACATGAGTGGAGCAATGGAGTTCCATGGCGGGTTTATGCGTGCTGGAAACCAGCACTTTGCTTCTGTTGTCCGGTGA

Protein Analysis

301

Amino Acids

33.21

Weight (kDa)

9.26

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 147 - 201 3.7e-27 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 795
AccB7I CCANNNNNTGG 1 cut(s) 27
AciI CCGC 3 cut(s) 95, 616, 855
AclWI GGATC 3 cut(s) 160, 370, 398
AcsI RAATTY 2 cut(s) 48, 725
AcuI CTGAAG 3 cut(s) 251, 322, 662
AfaI GTAC 4 cut(s) 396, 464, 646, 675
AfiI CCNNNNNNNGG 4 cut(s) 27, 101, 576, 577
AflIII ACRYGT 1 cut(s) 203
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 1 cut(s) 147
AleI CACNNNNGTG 1 cut(s) 831
AluBI AGCT 2 cut(s) 78, 530
AluI AGCT 2 cut(s) 78, 530
Alw26I GTCTC 2 cut(s) 478, 558
AlwI GGATC 3 cut(s) 160, 370, 398
ApeKI GCWGC 2 cut(s) 306, 793
ApoI RAATTY 2 cut(s) 48, 725
AspS9I GGNCC 1 cut(s) 437
AvaII GGWCC 1 cut(s) 437
BanII GRGCYC 1 cut(s) 590
BauI CACGAG 2 cut(s) 402, 548
BbvI GCAGC 2 cut(s) 293, 805
BccI CCATC 5 cut(s) 215, 428, 449, 567, 674
BceAI ACGGC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BciT130I CCWGG 1 cut(s) 149
BcoDI GTCTC 2 cut(s) 478, 558
BfaI CTAG 1 cut(s) 735
BfuAI ACCTGC 1 cut(s) 795
BisI GCNGC 3 cut(s) 307, 617, 794
BlsI GCNGC 3 cut(s) 308, 618, 795
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 825
BpmI CTGGAG 1 cut(s) 622
BpuEI CTTGAG 2 cut(s) 171, 264
BsaBI GATNNNNATC 1 cut(s) 157
BsaJI CCNNGG 4 cut(s) 66, 148, 466, 850
BsaWI WCCGGW 1 cut(s) 897
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 101, 576, 577
Bse1I ACTGG 2 cut(s) 236, 639
Bse3DI GCAATG 1 cut(s) 846
Bse8I GATNNNNATC 1 cut(s) 157
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 4 cut(s) 66, 148, 466, 850
BseGI GGATG 3 cut(s) 76, 158, 816
BseJI GATNNNNATC 1 cut(s) 157
BseLI CCNNNNNNNGG 4 cut(s) 27, 101, 576, 577
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 3 cut(s) 490, 619, 765
BseNI ACTGG 2 cut(s) 236, 639
BseXI GCAGC 2 cut(s) 293, 805
BsiSI CCGG 1 cut(s) 898
BslFI GGGAC 3 cut(s) 361, 475, 707
BslI CCNNNNNNNGG 4 cut(s) 27, 101, 576, 577
BsmAI GTCTC 2 cut(s) 478, 558
BsmFI GGGAC 3 cut(s) 361, 475, 707
Bsp1286I GDGCHC 1 cut(s) 590
Bsp143I GATC 5 cut(s) 152, 362, 390, 421, 799
Bsp19I CCATGG 1 cut(s) 850
BspACI CCGC 3 cut(s) 95, 616, 855
BspCNI CTCAG 3 cut(s) 489, 618, 764
BspHI TCATGA 2 cut(s) 109, 348
BspLI GGNNCC 1 cut(s) 439
BspMI ACCTGC 1 cut(s) 795
BspPI GGATC 3 cut(s) 160, 370, 398
BsrDI GCAATG 1 cut(s) 846
BsrI ACTGG 2 cut(s) 236, 639
BssECI CCNNGG 4 cut(s) 66, 148, 466, 850
BssMI GATC 5 cut(s) 152, 362, 390, 421, 799
BssSI CACGAG 2 cut(s) 402, 548
BssT1I CCWWGG 2 cut(s) 66, 850
Bst2BI CACGAG 2 cut(s) 402, 548
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 3 cut(s) 462, 467, 489
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 3 cut(s) 476, 605, 751
BstDSI CCRYGG 2 cut(s) 466, 850
BstF5I GGATG 3 cut(s) 76, 158, 816
BstKTI GATC 5 cut(s) 155, 365, 393, 424, 802
BstMAI GTCTC 2 cut(s) 478, 558
BstMBI GATC 5 cut(s) 152, 362, 390, 421, 799
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 293, 805
BstXI CCANNNNNNTGG 2 cut(s) 386, 833
BtgI CCRYGG 2 cut(s) 466, 850
BtsCI GGATG 3 cut(s) 76, 158, 816
BtsI GCAGTG 1 cut(s) 727
BtsIMutI CAGTG 2 cut(s) 346, 727
BveI ACCTGC 1 cut(s) 795
Cac8I GCNNGC 1 cut(s) 867
CciI TCATGA 2 cut(s) 109, 348
Cfr13I GGNCC 1 cut(s) 437
CseI GACGC 1 cut(s) 122
Csp6I GTAC 4 cut(s) 395, 463, 645, 674
CviAII CATG 8 cut(s) 22, 110, 204, 349, 566, 757, 829, 851
CviJI RGCY 9 cut(s) 78, 235, 306, 371, 509, 530, 538, 588, 796
CviKI_1 RGCY 9 cut(s) 78, 235, 306, 371, 509, 530, 538, 588, 796
CviQI GTAC 4 cut(s) 395, 463, 645, 674
DdeI CTNAG 3 cut(s) 476, 605, 751
DpnI GATC 5 cut(s) 154, 364, 392, 423, 801
DpnII GATC 5 cut(s) 152, 362, 390, 421, 799
EciI GGCGGA 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 66, 850
Eco24I GRGCYC 1 cut(s) 590
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 3 cut(s) 251, 322, 662
EcoRI GAATTC 1 cut(s) 725
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 2 cut(s) 66, 850
EcoT38I GRGCYC 1 cut(s) 590
ErhI CCWWGG 2 cut(s) 66, 850
FaeI CATG 8 cut(s) 25, 113, 207, 352, 569, 760, 832, 854
FaqI GGGAC 3 cut(s) 361, 475, 707
FatI CATG 8 cut(s) 21, 109, 203, 348, 565, 756, 828, 850
FauI CCCGC 1 cut(s) 848
FauNDI CATATG 2 cut(s) 358, 381
Fnu4HI GCNGC 3 cut(s) 307, 617, 794
FokI GGATG 3 cut(s) 83, 145, 803
FriOI GRGCYC 1 cut(s) 590
Fsp4HI GCNGC 3 cut(s) 307, 617, 794
FspBI CTAG 1 cut(s) 735
GluI GCNGC 3 cut(s) 307, 617, 794
GsuI CTGGAG 1 cut(s) 622
HapII CCGG 1 cut(s) 898
HgaI GACGC 1 cut(s) 122
Hin1II CATG 8 cut(s) 25, 113, 207, 352, 569, 760, 832, 854
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 5 cut(s) 31, 56, 471, 551, 662
HpaII CCGG 1 cut(s) 898
Hpy166II GTNNAC 4 cut(s) 41, 181, 328, 596
Hpy188I TCNGA 7 cut(s) 55, 479, 625, 661, 681, 715, 754
Hpy188III TCNNGA 4 cut(s) 110, 257, 349, 419
Hpy8I GTNNAC 4 cut(s) 41, 181, 328, 596
HpyAV CCTTC 2 cut(s) 285, 474
HpyCH4III ACNGT 3 cut(s) 462, 467, 489
HpyCH4IV ACGT 2 cut(s) 598, 654
HpyCH4V TGCA 5 cut(s) 309, 356, 455, 497, 743
HpyF3I CTNAG 3 cut(s) 476, 605, 751
HpySE526I ACGT 2 cut(s) 598, 654
Hsp92II CATG 8 cut(s) 25, 113, 207, 352, 569, 760, 832, 854
Kzo9I GATC 5 cut(s) 152, 362, 390, 421, 799
LmnI GCTCC 1 cut(s) 836
Lsp1109I GCAGC 2 cut(s) 293, 805
LweI GCATC 1 cut(s) 825
MaeI CTAG 1 cut(s) 735
MaeII ACGT 2 cut(s) 598, 654
MaeIII GTNAC 1 cut(s) 287
MalI GATC 5 cut(s) 154, 364, 392, 423, 801
MboI GATC 5 cut(s) 152, 362, 390, 421, 799
MboII GAAGA 2 cut(s) 206, 554
MfeI CAATTG 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 590
MluCI AATT 6 cut(s) 16, 48, 174, 295, 725, 744
MlyI GAGTC 3 cut(s) 65, 560, 656
MmeI TCCRAC 2 cut(s) 483, 795
MnlI CCTC 6 cut(s) 426, 485, 499, 646, 740, 831
MseI TTAA 2 cut(s) 132, 263
MslI CAYNNNNRTG 2 cut(s) 564, 831
MspI CCGG 1 cut(s) 898
MspR9I CCNGG 1 cut(s) 149
MunI CAATTG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 149
NcoI CCATGG 1 cut(s) 850
NdeI CATATG 2 cut(s) 358, 381
NdeII GATC 5 cut(s) 152, 362, 390, 421, 799
NlaIII CATG 8 cut(s) 25, 113, 207, 352, 569, 760, 832, 854
NlaIV GGNNCC 1 cut(s) 439
NmeAIII GCCGAG 1 cut(s) 273
NspI RCATGY 1 cut(s) 207
OliI CACNNNNGTG 1 cut(s) 831
PagI TCATGA 2 cut(s) 109, 348
PciI ACATGT 1 cut(s) 203
PfeI GAWTC 2 cut(s) 31, 471
PflMI CCANNNNNTGG 1 cut(s) 27
PkrI GCNGC 3 cut(s) 308, 618, 795
PleI GAGTC 3 cut(s) 64, 559, 656
PpsI GAGTC 3 cut(s) 64, 559, 656
PscI ACATGT 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 437
RsaI GTAC 4 cut(s) 396, 464, 646, 675
RsaNI GTAC 4 cut(s) 395, 463, 645, 674
RseI CAYNNNNRTG 2 cut(s) 564, 831
SaqAI TTAA 2 cut(s) 132, 263
SatI GCNGC 3 cut(s) 307, 617, 794
Sau3AI GATC 5 cut(s) 152, 362, 390, 421, 799
Sau96I GGNCC 1 cut(s) 437
SchI GAGTC 3 cut(s) 65, 560, 656
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 1 cut(s) 590
SetI ASST 7 cut(s) 80, 106, 400, 532, 601, 657, 809
SfaNI GCATC 1 cut(s) 825
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 564, 831
SmlI CTYRAG 2 cut(s) 186, 279
SmoI CTYRAG 2 cut(s) 186, 279
Sse9I AATT 6 cut(s) 16, 48, 174, 295, 725, 744
SsiI CCGC 3 cut(s) 95, 616, 855
SspMI CTAG 1 cut(s) 735
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 2 cut(s) 66, 850
TaaI ACNGT 3 cut(s) 462, 467, 489
TaiI ACGT 2 cut(s) 601, 657
TaqI TCGA 2 cut(s) 269, 532
TasI AATT 6 cut(s) 16, 48, 174, 295, 725, 744
TauI GCSGC 1 cut(s) 619
TfiI GAWTC 2 cut(s) 31, 471
Tru1I TTAA 2 cut(s) 132, 263
Tru9I TTAA 2 cut(s) 132, 263
TscAI CASTG 2 cut(s) 346, 727
TseI GCWGC 2 cut(s) 306, 793
TspDTI ATGAA 3 cut(s) 213, 337, 554
TspRI CASTG 2 cut(s) 346, 727
Van91I CCANNNNNTGG 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 2 cut(s) 48, 725
XceI RCATGY 1 cut(s) 207
XspI CTAG 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.