Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0016026

Physical Location & Seq

Genomic Coordinates Reverse (-)

10320629 .. 10323181

Gene Structure 2553 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1127500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1146 bp

ATGTTGAAGCTCTCGACGTACAGTGGAGTGGACCAAATTCACTGCGTGCCGGCCCAATGCCGCTTCCTGGGCCCACCCAAGCCCAACCCAATTTTCCCCCGCCGGAAAGACGGCCCATTTCTTGCCCCCTGCGCTGCTTCCCCTTCCAAAACACCGGTTCTCGCCGTCGTATCGGACCGGGGGGCCGGAAGCGTGGCGGGACGGGTCGAGACGAAATCCGGGTCGGTCACCCTGGCAAACCGGTTGAGGAAGGGGAGCATGGCGGATGACGGGAAATCGTACAAGGAGTGCTTTATAGTGAGGTCCTATGAGGTCGGGATTAACAAGACTGCCACTGTCGAGACGATTGCTAATTTCATCCAGGAGGTAGGATGCAACCATGCTCAGTATGTTGGCTTTTCGACCGATGGATTTGCGACCACCACTACCATGAGGGAAATGCATCTCATATGGGTTACTGCTCGCATGCATATTGAAATCTACAAATATCCAGCTTGGTGGGTGAGTGATGTCGTTGAAATAGAAACATGGTGCCAAGGGGAAGGGAGAATCGGCACTAGACGCGATTGGATACTCAAGGACTATGCCACCGGTGAAGTCATTGGAAGAGCGACAAGCAAGTGGGTGATGATGAACCAAGACACTAGGCGACTTCAGAAAGTCAGCGACGATGTTAGGGAAGAGCACTTAGTTTTTGCTCCGCGAGAACTGAGATTAGCGTTTCCAGAACCTAACAATAATAGCTTGAGGAAAATTCCCAAACTGGAAGATCCTGCACAGTATTCCAGACTGGGAGTTGTGCCGAGGAGAGCTGATCTTGACATGAATCAACATGTTAATAATGTTGCTTACATCGGATGGGTTCTTGAGAGCATGCCTCAAGAACTCATTGATAGCCATGAACTGGAAGCAATCACCTTAGATTACAGGCGGGAGTGTCAACGGGATGACATGGTTGATTCCCTTACCAGCGTTGAACCTTTCGATGATGCTCCAGCTCTTTCAGGTAACGGAACAAACGGATCCCTTGCTGCAACAGAAGATGATAAGGACTACCGTCAGTATTTGCATCTACTAAGATCAGCGGGCGATGATTCAGAAATAAACCGAGGGCGAACTATCTGGAGAGAAAAACCTGCAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004312 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016297 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 map00061

Pfam Domains

Protein Families

Acyl-ACP_TE

Protein Analysis

382

Amino Acids

42.94

Weight (kDa)

6.28

Isoelectric Point (pI)

41.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-ACP_TE	PF01643	91 - 228	6.6e-41	Acyl-ACP thioesterase N-terminal domain
Acyl-ACP_TE_C	PF20791	257 - 377	6e-25	Acyl-ACP thioesterase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016026)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25110
fragaria_vesca	FvH4_2g18600
malus_domestica	MD02G1127500.v1.1 MD05G1172000.v1.1
prunus_persica	Prupe.8G199400_v2.0.a1
pyrus_communis	pycom02g09960 pycom05g15870
rosa_chinensis	RchiOBHm_Chr6g0284171
rosa_laevigata	RLG00000012762
rosa_multiflora	Rmu_sc0003778.1_g000013
rosa_roxburghii	Rroxscaffold_7G00184830
rosa_rugosa	Rorug06G0161500
rosa_samantha	Rh6BG274800 Rh6DG269200
rosa_wichuraiana	Rw6G023590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	531
AccB7I	CCANNNNNTGG	1 cut(s)	904
AccII	CGCG	2 cut(s)	564, 703
AciI	CCGC	7 cut(s)	61, 100, 197, 263, 701, 931, 1085
AclWI	GGATC	3 cut(s)	764, 1017, 1030
AcsI	RAATTY	2 cut(s)	36, 753
AcuI	CTGAAG	1 cut(s)	638
AdeI	CACNNNGTG	1 cut(s)	46
AfaI	GTAC	2 cut(s)	20, 281
AfiI	CCNNNNNNNGG	2 cut(s)	67, 904
AflIII	ACRYGT	1 cut(s)	832
AgeI	ACCGGT	3 cut(s)	154, 240, 590
AgsI	TTSAA	4 cut(s)	7, 476, 518, 977
AjnI	CCWGG	3 cut(s)	66, 231, 360
AjuI	GAANNNNNNNTTGG	2 cut(s)	77, 109
AluBI	AGCT	5 cut(s)	10, 494, 744, 812, 998
AluI	AGCT	5 cut(s)	10, 494, 744, 812, 998
Alw21I	GWGCWC	1 cut(s)	687
Alw26I	GTCTC	2 cut(s)	203, 335
AlwI	GGATC	3 cut(s)	764, 1017, 1030
AoxI	GGCC	4 cut(s)	51, 70, 112, 183
ApaI	GGGCCC	1 cut(s)	74
ApeKI	GCWGC	2 cut(s)	134, 1031
ApoI	RAATTY	2 cut(s)	36, 753
ArsI	GACNNNNNNTTYG	2 cut(s)	206, 238
AsiGI	ACCGGT	3 cut(s)	154, 240, 590
AspLEI	GCGC	1 cut(s)	134
AspS9I	GGNCC	8 cut(s)	31, 52, 70, 71, 113, 175, 183, 303
AsuC2I	CCSGG	2 cut(s)	179, 220
AsuHPI	GGTGA	5 cut(s)	220, 514, 605, 637, 907
AvaII	GGWCC	3 cut(s)	31, 175, 303
BaeGI	GKGCMC	1 cut(s)	74
BamHI	GGATCC	1 cut(s)	1022
BanI	GGYRCC	1 cut(s)	531
BanII	GRGCYC	1 cut(s)	74
Bbv12I	GWGCWC	1 cut(s)	687
BbvI	GCAGC	2 cut(s)	121, 1018
BccI	CCATC	2 cut(s)	401, 852
BceAI	ACGGC	2 cut(s)	127, 149
BcgI	CGANNNNNNTGC	4 cut(s)	329, 363, 395, 429
BciT130I	CCWGG	3 cut(s)	68, 233, 362
BciVI	GTATCC	1 cut(s)	564
BcnI	CCSGG	2 cut(s)	179, 220
BcoDI	GTCTC	2 cut(s)	203, 335
BfaI	CTAG	2 cut(s)	558, 645
BfuI	GTATCC	1 cut(s)	564
BisI	GCNGC	3 cut(s)	61, 135, 1032
BlsI	GCNGC	3 cut(s)	62, 136, 1033
Bme1390I	CCNGG	5 cut(s)	68, 179, 220, 233, 362
Bme18I	GGWCC	3 cut(s)	31, 175, 303
BmgT120I	GGNCC	8 cut(s)	31, 52, 70, 71, 113, 175, 183, 303
BmiI	GGNNCC	4 cut(s)	72, 184, 533, 1024
BmrFI	CCNGG	5 cut(s)	68, 179, 220, 233, 362
BmrI	ACTGGG	1 cut(s)	800
BmsI	GCATC	4 cut(s)	362, 451, 979, 1078
BmuI	ACTGGG	1 cut(s)	800
BoxI	GACNNNNGTC	1 cut(s)	1056
BplI	GAGNNNNNCTC	2 cut(s)	862, 894
BpmI	CTGGAG	2 cut(s)	978, 1144
BpuEI	CTTGAG	4 cut(s)	560, 766, 864, 887
BpuMI	CCSGG	2 cut(s)	179, 220
BsaJI	CCNNGG	6 cut(s)	67, 178, 231, 535, 803, 1108
BsaWI	WCCGGW	3 cut(s)	154, 240, 590
Bsc4I	CCNNNNNNNGG	2 cut(s)	67, 904
Bse118I	RCCGGY	4 cut(s)	49, 154, 240, 590
Bse1I	ACTGG	3 cut(s)	768, 795, 909
BseBI	CCWGG	3 cut(s)	68, 233, 362
BseDI	CCNNGG	6 cut(s)	67, 178, 231, 535, 803, 1108
BseGI	GGATG	5 cut(s)	271, 357, 377, 863, 952
BseLI	CCNNNNNNNGG	2 cut(s)	67, 904
BseMII	CTCAG	2 cut(s)	398, 701
BseNI	ACTGG	3 cut(s)	768, 795, 909
BseRI	GAGGAG	1 cut(s)	820
BseSI	GKGCMC	1 cut(s)	74
BseXI	GCAGC	2 cut(s)	121, 1018
BsgI	GTGCAG	1 cut(s)	759
Bsh1236I	CGCG	2 cut(s)	564, 703
Bsh1285I	CGRYCG	1 cut(s)	405
BshFI	GGCC	4 cut(s)	53, 72, 114, 185
BshNI	GGYRCC	1 cut(s)	531
BshTI	ACCGGT	3 cut(s)	154, 240, 590
BsiEI	CGRYCG	1 cut(s)	405
BsiHKAI	GWGCWC	1 cut(s)	687
BsiSI	CCGG	8 cut(s)	50, 103, 155, 178, 186, 219, 241, 591
BslFI	GGGAC	1 cut(s)	213
BslI	CCNNNNNNNGG	2 cut(s)	67, 904
BsmAI	GTCTC	2 cut(s)	203, 335
BsmBI	CGTCTC	2 cut(s)	203, 335
BsmFI	GGGAC	1 cut(s)	213
BsnI	GGCC	4 cut(s)	53, 72, 114, 185
Bsp120I	GGGCCC	1 cut(s)	70
Bsp1286I	GDGCHC	2 cut(s)	74, 687
Bsp143I	GATC	4 cut(s)	769, 814, 1022, 1079
BspACI	CCGC	7 cut(s)	61, 100, 197, 263, 701, 931, 1085
BspANI	GGCC	4 cut(s)	53, 72, 114, 185
BspCNI	CTCAG	2 cut(s)	397, 702
BspFNI	CGCG	2 cut(s)	564, 703
BspLI	GGNNCC	4 cut(s)	72, 184, 533, 1024
BspPI	GGATC	3 cut(s)	764, 1017, 1030
BspQI	GCTCTTC	2 cut(s)	601, 675
BspT107I	GGYRCC	1 cut(s)	531
BsrFI	RCCGGY	4 cut(s)	49, 154, 240, 590
BsrI	ACTGG	3 cut(s)	768, 795, 909
BssAI	RCCGGY	4 cut(s)	49, 154, 240, 590
BssECI	CCNNGG	6 cut(s)	67, 178, 231, 535, 803, 1108
BssMI	GATC	4 cut(s)	769, 814, 1022, 1079
BssT1I	CCWWGG	1 cut(s)	535
Bst2UI	CCWGG	3 cut(s)	68, 233, 362
Bst4CI	ACNGT	4 cut(s)	23, 337, 780, 1058
Bst6I	CTCTTC	2 cut(s)	601, 675
BstC8I	GCNNGC	6 cut(s)	47, 51, 463, 467, 875, 1087
BstDEI	CTNAG	5 cut(s)	384, 688, 710, 919, 1076
BstEII	GGTNACC	1 cut(s)	226
BstF5I	GGATG	5 cut(s)	271, 357, 377, 863, 952
BstFNI	CGCG	2 cut(s)	564, 703
BstHHI	GCGC	1 cut(s)	134
BstKTI	GATC	4 cut(s)	772, 817, 1025, 1082
BstMAI	GTCTC	2 cut(s)	203, 335
BstMBI	GATC	4 cut(s)	769, 814, 1022, 1079
BstMCI	CGRYCG	1 cut(s)	405
BstMWI	GCNNNNNNNGC	3 cut(s)	69, 131, 561
BstNI	CCWGG	3 cut(s)	68, 233, 362
BstNSI	RCATGY	3 cut(s)	469, 836, 877
BstPAI	GACNNNNGTC	1 cut(s)	1056
BstPI	GGTNACC	1 cut(s)	226
BstSCI	CCNGG	5 cut(s)	66, 177, 218, 231, 360
BstSLI	GKGCMC	1 cut(s)	74
BstUI	CGCG	2 cut(s)	564, 703
BstV1I	GCAGC	2 cut(s)	121, 1018
BstX2I	RGATCY	2 cut(s)	769, 1022
BstXI	CCANNNNNNTGG	1 cut(s)	498
BstYI	RGATCY	2 cut(s)	769, 1022
BsuI	GTATCC	1 cut(s)	564
BsuRI	GGCC	4 cut(s)	53, 72, 114, 185
BtgZI	GCGATG	1 cut(s)	1104
BtsCI	GGATG	5 cut(s)	271, 357, 377, 863, 952
BtsI	GCAGTG	1 cut(s)	40
BtsIMutI	CAGTG	3 cut(s)	28, 40, 333
Cac8I	GCNNGC	6 cut(s)	47, 51, 463, 467, 875, 1087
CfoI	GCGC	1 cut(s)	134
Cfr10I	RCCGGY	4 cut(s)	49, 154, 240, 590
Cfr13I	GGNCC	8 cut(s)	31, 52, 70, 71, 113, 175, 183, 303
CpoI	CGGWCCG	1 cut(s)	175
CseI	GACGC	1 cut(s)	570
Csp6I	GTAC	2 cut(s)	19, 280
CspAI	ACCGGT	3 cut(s)	154, 240, 590
CspI	CGGWCCG	1 cut(s)	175
CviQI	GTAC	2 cut(s)	19, 280
DdeI	CTNAG	5 cut(s)	384, 688, 710, 919, 1076
DpnI	GATC	4 cut(s)	771, 816, 1024, 1081
DpnII	GATC	4 cut(s)	769, 814, 1022, 1079
DraIII	CACNNNGTG	1 cut(s)	46
Eam1104I	CTCTTC	2 cut(s)	601, 675
EarI	CTCTTC	2 cut(s)	601, 675
EciI	GGCGGA	1 cut(s)	278
Eco130I	CCWWGG	1 cut(s)	535
Eco24I	GRGCYC	1 cut(s)	74
Eco47I	GGWCC	3 cut(s)	31, 175, 303
Eco57I	CTGAAG	1 cut(s)	638
Eco91I	GGTNACC	1 cut(s)	226
EcoO109I	RGGNCCY	1 cut(s)	303
EcoO65I	GGTNACC	1 cut(s)	226
EcoRII	CCWGG	3 cut(s)	66, 231, 360
EcoT14I	CCWWGG	1 cut(s)	535
EcoT22I	ATGCAT	2 cut(s)	444, 471
EcoT38I	GRGCYC	1 cut(s)	74
ErhI	CCWWGG	1 cut(s)	535
Esp3I	CGTCTC	2 cut(s)	203, 335
FalI	AAGNNNNNCTT	2 cut(s)	275, 307
FaqI	GGGAC	1 cut(s)	213
FauI	CCCGC	4 cut(s)	107, 190, 924, 1078
FauNDI	CATATG	1 cut(s)	449
Fnu4HI	GCNGC	3 cut(s)	61, 135, 1032
FokI	GGATG	5 cut(s)	278, 344, 384, 870, 959
FriOI	GRGCYC	1 cut(s)	74
Fsp4HI	GCNGC	3 cut(s)	61, 135, 1032
FspBI	CTAG	2 cut(s)	558, 645
GlaI	GCGC	1 cut(s)	133
GluI	GCNGC	3 cut(s)	61, 135, 1032
GsuI	CTGGAG	2 cut(s)	978, 1144
HaeIII	GGCC	4 cut(s)	53, 72, 114, 185
HapII	CCGG	8 cut(s)	50, 103, 155, 178, 186, 219, 241, 591
HgaI	GACGC	1 cut(s)	570
HhaI	GCGC	1 cut(s)	134
Hin6I	GCGC	1 cut(s)	132
HinP1I	GCGC	1 cut(s)	132
HincII	GTYRAC	1 cut(s)	941
HindII	GTYRAC	1 cut(s)	941
HinfI	GANTC	4 cut(s)	549, 826, 959, 1094
HpaII	CCGG	8 cut(s)	50, 103, 155, 178, 186, 219, 241, 591
HphI	GGTGA	5 cut(s)	220, 514, 605, 637, 907
Hpy166II	GTNNAC	2 cut(s)	31, 941
Hpy188I	TCNGA	4 cut(s)	175, 657, 857, 1099
Hpy8I	GTNNAC	2 cut(s)	31, 941
Hpy99I	CGWCG	3 cut(s)	19, 170, 671
HpyAV	CCTTC	3 cut(s)	153, 244, 536
HpyCH4III	ACNGT	4 cut(s)	23, 337, 780, 1058
HpyCH4IV	ACGT	1 cut(s)	17
HpyCH4V	TGCA	7 cut(s)	375, 442, 469, 776, 1034, 1069, 1139
HpyF10VI	GCNNNNNNNGC	3 cut(s)	69, 131, 561
HpyF3I	CTNAG	5 cut(s)	384, 688, 710, 919, 1076
HpySE526I	ACGT	1 cut(s)	17
HspAI	GCGC	1 cut(s)	132
KroI	GCCGGC	1 cut(s)	49
KroNI	GCCGGC	1 cut(s)	51
Kzo9I	GATC	4 cut(s)	769, 814, 1022, 1079
LguI	GCTCTTC	2 cut(s)	601, 675
LmnI	GCTCC	3 cut(s)	255, 703, 997
Lsp1109I	GCAGC	2 cut(s)	121, 1018
LweI	GCATC	4 cut(s)	362, 451, 979, 1078
MaeI	CTAG	2 cut(s)	558, 645
MaeII	ACGT	1 cut(s)	17
MaeIII	GTNAC	3 cut(s)	226, 454, 1007
MalI	GATC	4 cut(s)	771, 816, 1024, 1081
MboI	GATC	4 cut(s)	769, 814, 1022, 1079
MboII	GAAGA	4 cut(s)	618, 692, 779, 1052
MflI	RGATCY	2 cut(s)	769, 1022
MhlI	GDGCHC	2 cut(s)	74, 687
MluCI	AATT	4 cut(s)	36, 90, 352, 753
MnlI	CCTC	9 cut(s)	240, 294, 304, 358, 426, 741, 798, 888, 1103
Mph1103I	ATGCAT	2 cut(s)	444, 471
MroNI	GCCGGC	1 cut(s)	49
MseI	TTAA	2 cut(s)	321, 837
MslI	CAYNNNNRTG	1 cut(s)	428
MspA1I	CMGCKG	1 cut(s)	1085
MspI	CCGG	8 cut(s)	50, 103, 155, 178, 186, 219, 241, 591
MspR9I	CCNGG	5 cut(s)	68, 179, 220, 233, 362
MvaI	CCWGG	3 cut(s)	68, 233, 362
MvnI	CGCG	2 cut(s)	564, 703
MwoI	GCNNNNNNNGC	3 cut(s)	69, 131, 561
NaeI	GCCGGC	1 cut(s)	51
NciI	CCSGG	2 cut(s)	179, 220
NdeI	CATATG	1 cut(s)	449
NdeII	GATC	4 cut(s)	769, 814, 1022, 1079
NgoMIV	GCCGGC	1 cut(s)	49
NlaIV	GGNNCC	4 cut(s)	72, 184, 533, 1024
NmeAIII	GCCGAG	1 cut(s)	828
NmuCI	GTSAC	1 cut(s)	226
NsiI	ATGCAT	2 cut(s)	444, 471
NspI	RCATGY	3 cut(s)	469, 836, 877
PaeI	GCATGC	2 cut(s)	469, 877
PciI	ACATGT	1 cut(s)	832
PciSI	GCTCTTC	2 cut(s)	601, 675
PdiI	GCCGGC	1 cut(s)	51
PfeI	GAWTC	4 cut(s)	549, 826, 959, 1094
PflMI	CCANNNNNTGG	1 cut(s)	904
PfoI	TCCNGGA	1 cut(s)	360
PinAI	ACCGGT	3 cut(s)	154, 240, 590
PkrI	GCNGC	3 cut(s)	62, 136, 1033
PpuMI	RGGWCCY	1 cut(s)	303
PscI	ACATGT	1 cut(s)	832
PshAI	GACNNNNGTC	1 cut(s)	1056
Psp5II	RGGWCCY	1 cut(s)	303
Psp6I	CCWGG	3 cut(s)	66, 231, 360
PspEI	GGTNACC	1 cut(s)	226
PspGI	CCWGG	3 cut(s)	66, 231, 360
PspN4I	GGNNCC	4 cut(s)	72, 184, 533, 1024
PspOMI	GGGCCC	1 cut(s)	70
PspPI	GGNCC	8 cut(s)	31, 52, 70, 71, 113, 175, 183, 303
PspPPI	RGGWCCY	1 cut(s)	303
PsuI	RGATCY	2 cut(s)	769, 1022
RsaI	GTAC	2 cut(s)	20, 281
RsaNI	GTAC	2 cut(s)	19, 280
RseI	CAYNNNNRTG	1 cut(s)	428
Rsr2I	CGGWCCG	1 cut(s)	175
RsrII	CGGWCCG	1 cut(s)	175
SapI	GCTCTTC	2 cut(s)	601, 675
SaqAI	TTAA	2 cut(s)	321, 837
SatI	GCNGC	3 cut(s)	61, 135, 1032
Sau3AI	GATC	4 cut(s)	769, 814, 1022, 1079
Sau96I	GGNCC	8 cut(s)	31, 52, 70, 71, 113, 175, 183, 303
ScrFI	CCNGG	5 cut(s)	68, 179, 220, 233, 362
SduI	GDGCHC	2 cut(s)	74, 687
SfaNI	GCATC	4 cut(s)	362, 451, 979, 1078
SgrAI	CRCCGGYG	1 cut(s)	590
SinI	GGWCC	3 cut(s)	31, 175, 303
SmiMI	CAYNNNNRTG	1 cut(s)	428
SmlI	CTYRAG	4 cut(s)	575, 745, 866, 879
SmoI	CTYRAG	4 cut(s)	575, 745, 866, 879
SphI	GCATGC	2 cut(s)	469, 877
Sse9I	AATT	4 cut(s)	36, 90, 352, 753
SsiI	CCGC	7 cut(s)	61, 100, 197, 263, 701, 931, 1085
SspMI	CTAG	2 cut(s)	558, 645
StyD4I	CCNGG	5 cut(s)	66, 177, 218, 231, 360
StyI	CCWWGG	1 cut(s)	535
TaaI	ACNGT	4 cut(s)	23, 337, 780, 1058
TaiI	ACGT	1 cut(s)	20
TaqI	TCGA	5 cut(s)	14, 207, 339, 401, 984
TaqII	GACCGA	2 cut(s)	214, 419
TasI	AATT	4 cut(s)	36, 90, 352, 753
TauI	GCSGC	1 cut(s)	63
TfiI	GAWTC	4 cut(s)	549, 826, 959, 1094
Tru1I	TTAA	2 cut(s)	321, 837
Tru9I	TTAA	2 cut(s)	321, 837
TscAI	CASTG	3 cut(s)	28, 47, 340
TseFI	GTSAC	1 cut(s)	226
TseI	GCWGC	2 cut(s)	134, 1031
Tsp45I	GTSAC	1 cut(s)	226
TspDTI	ATGAA	4 cut(s)	346, 647, 839, 915
TspGWI	ACGGA	2 cut(s)	1026, 1035
TspRI	CASTG	3 cut(s)	28, 47, 340
Van91I	CCANNNNNTGG	1 cut(s)	904
VpaK11BI	GGWCC	3 cut(s)	31, 175, 303
XapI	RAATTY	2 cut(s)	36, 753
XceI	RCATGY	3 cut(s)	469, 836, 877
XspI	CTAG	2 cut(s)	558, 645
Zsp2I	ATGCAT	2 cut(s)	444, 471

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.