Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0016026

Physical Location & Seq

Genomic Coordinates Forward (+)

30078988 .. 30082280

Gene Structure 3293 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1172000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGTTGAAGCTCTCGACATACAATGGAGTGGACCAAATTCACGGCGTGGCGGCCCAATGCCGCTTCCTTGGCCCACCCAAGCCCAACCCAATTTTCCCCCGCCGGAAAGACGGCGCCTTTCTTGCCCGCTGCGCTGCCTCACCTTCCAAAACGCCGGTTCTCGCCGTCGTATCGGACCGGGGGCCCGGGAGCTTGGCGGGTCGGGTCGAAACGAATACCGGGCCGGTCACCCTCGCGGACCGGCTGAGGCAGGGGAGCATGGCGGAGGACGGGAAGTCCTATAAGGAGTGCTTTATAGTGAGGTGCTATGAGGTCGGGATTAACAAGACTGCCACCGTCGAGACGATTGCCAATCTCTTGCAGGAGGTAGGATGCAACCATGCTCAAGCTGTTGGCTTTTCTACTGACGGATTTGCGACCACAACCACCATGAGAAAAATGCATCTCATTTGGGTTACTGCTCGCATGCATATTGAAATCTACAAATATCCAGCTTGGAGTGATGTCGTTGAAATAGAAACATGGTGCCAAGGGGAAGGGAGAATTGGCACTAGGCGTGATTGGATACTCAAGGACTATGCCACCGGTGAAGTCATTGGAAGAGCGACGAGCAAGTGGGTGATGATGAACCAAGACACTAGGCGACTTGTGAAAGTCACCGATGATGTTAGGGAAGAGCATTTAGTTTTTGCTCCACGAGAACTGAGATTAGCATTTCCAGAGCCAAACAATAGTAGCTTAAAGAAAATCTCCAAACTTGAAGATCCTGCTCAGTATTCTACACTGGGACTTGTGCCAAGGAGAGCAGATCTCGACATGAACCAGCATGTTAATAATGTCGCCTATATCGGATGGGTTCTAGAGAGCATGCCTCAAGAACTCATCGATAGCCATGAACTGCAAACAATCACCTTAGATTACAGGCGGGAGTGTCAACAGGATGACATAGTCAATTCCCTTACTAGTGTTGAACCTTTAGACGATGCTCCAGCTCTTTCAGGTAACGGAACAAATGGATCCCCTACTGCAACAGAAGATGATAAGGACTACCGTCAGTATTTGCATCTACTAAGATTAGCAGGCGATGGTTCGGAAATAAACCGGGGACGAACTATCTGGAGAGAAAAACCTGCAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004312 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016297 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 map00061

Pfam Domains

Protein Families

Acyl-ACP_TE

Protein Analysis

380

Amino Acids

42.4

Weight (kDa)

6.47

Isoelectric Point (pI)

40.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-ACP_TE	PF01643	91 - 226	3.7e-44	Acyl-ACP thioesterase N-terminal domain
Acyl-ACP_TE_C	PF20791	255 - 375	5.6e-26	Acyl-ACP thioesterase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016026)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25110
fragaria_vesca	FvH4_2g18600
malus_domestica	MD02G1127500.v1.1 MD05G1172000.v1.1
prunus_persica	Prupe.8G199400_v2.0.a1
pyrus_communis	pycom02g09960 pycom05g15870
rosa_chinensis	RchiOBHm_Chr6g0284171
rosa_laevigata	RLG00000012762
rosa_multiflora	Rmu_sc0003778.1_g000013
rosa_roxburghii	Rroxscaffold_7G00184830
rosa_rugosa	Rorug06G0161500
rosa_samantha	Rh6BG274800 Rh6DG269200
rosa_wichuraiana	Rw6G023590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	113, 525
AccII	CGCG	1 cut(s)	236
AciI	CCGC	8 cut(s)	50, 61, 100, 127, 197, 236, 263, 925
AclWI	GGATC	3 cut(s)	758, 1011, 1024
AcsI	RAATTY	1 cut(s)	36
AcyI	GRCGYC	1 cut(s)	114
AdeI	CACNNNGTG	1 cut(s)	46
AgeI	ACCGGT	1 cut(s)	584
AgsI	TTSAA	5 cut(s)	7, 476, 512, 761, 971
AhdI	GACNNNNNGTC	1 cut(s)	274
AhlI	ACTAGT	1 cut(s)	962
AjuI	GAANNNNNNNTTGG	4 cut(s)	77, 109, 528, 560
AluBI	AGCT	6 cut(s)	10, 192, 389, 494, 738, 992
AluI	AGCT	6 cut(s)	10, 192, 389, 494, 738, 992
Alw26I	GTCTC	1 cut(s)	335
AlwI	GGATC	3 cut(s)	758, 1011, 1024
Ama87I	CYCGRG	1 cut(s)	185
AoxI	GGCC	4 cut(s)	51, 70, 182, 221
ApaI	GGGCCC	1 cut(s)	186
ApeKI	GCWGC	2 cut(s)	129, 134
ApoI	RAATTY	1 cut(s)	36
AsiGI	ACCGGT	1 cut(s)	584
AspLEI	GCGC	2 cut(s)	116, 134
AspS9I	GGNCC	8 cut(s)	31, 52, 71, 175, 182, 183, 221, 238
AsuC2I	CCSGG	5 cut(s)	179, 186, 187, 220, 1103
AsuHPI	GGTGA	6 cut(s)	132, 220, 599, 631, 649, 901
AvaI	CYCGRG	1 cut(s)	185
AvaII	GGWCC	3 cut(s)	31, 175, 238
BaeGI	GKGCMC	1 cut(s)	186
BamHI	GGATCC	1 cut(s)	1016
BanI	GGYRCC	2 cut(s)	113, 525
BanII	GRGCYC	1 cut(s)	186
BauI	CACGAG	1 cut(s)	696
BbvCI	CCTCAGC	1 cut(s)	245
BbvI	GCAGC	2 cut(s)	116, 121
BccI	CCATC	2 cut(s)	846, 1079
BceAI	ACGGC	3 cut(s)	58, 127, 149
BcgI	CGANNNNNNTGC	2 cut(s)	329, 363
BciVI	GTATCC	1 cut(s)	558
BcnI	CCSGG	5 cut(s)	179, 186, 187, 220, 1103
BcoDI	GTCTC	1 cut(s)	335
BcuI	ACTAGT	1 cut(s)	962
BfaI	CTAG	4 cut(s)	552, 639, 860, 963
BfoI	RGCGCY	1 cut(s)	117
BfuI	GTATCC	1 cut(s)	558
BglII	AGATCT	1 cut(s)	808
BisI	GCNGC	4 cut(s)	51, 61, 130, 135
BlsI	GCNGC	4 cut(s)	52, 62, 131, 136
Bme1390I	CCNGG	5 cut(s)	179, 186, 187, 220, 1103
Bme18I	GGWCC	3 cut(s)	31, 175, 238
BmeRI	GACNNNNNGTC	1 cut(s)	274
BmeT110I	CYCGRG	1 cut(s)	185
BmgT120I	GGNCC	8 cut(s)	31, 52, 71, 175, 182, 183, 221, 238
BmiI	GGNNCC	5 cut(s)	115, 183, 184, 527, 1018
BmrFI	CCNGG	5 cut(s)	179, 186, 187, 220, 1103
BmrI	ACTGGG	1 cut(s)	794
BmsI	GCATC	4 cut(s)	362, 451, 973, 1072
BmuI	ACTGGG	1 cut(s)	794
BoxI	GACNNNNGTC	1 cut(s)	1050
BplI	GAGNNNNNCTC	4 cut(s)	795, 827, 856, 888
BpmI	CTGGAG	2 cut(s)	972, 1138
Bpu10I	CCTNAGC	1 cut(s)	245
BpuEI	CTTGAG	3 cut(s)	369, 554, 858
BpuMI	CCSGG	5 cut(s)	179, 186, 187, 220, 1103
Bsa29I	ATCGAT	1 cut(s)	885
BsaHI	GRCGYC	1 cut(s)	114
BsaJI	CCNNGG	6 cut(s)	67, 178, 185, 529, 797, 1102
BsaWI	WCCGGW	1 cut(s)	584
Bse118I	RCCGGY	4 cut(s)	154, 223, 240, 584
Bse1I	ACTGG	1 cut(s)	789
BseCI	ATCGAT	1 cut(s)	885
BseDI	CCNNGG	6 cut(s)	67, 178, 185, 529, 797, 1102
BseGI	GGATG	3 cut(s)	377, 857, 946
BseMII	CTCAG	3 cut(s)	236, 695, 785
BseNI	ACTGG	1 cut(s)	789
BseSI	GKGCMC	1 cut(s)	186
BseXI	GCAGC	2 cut(s)	116, 121
Bsh1236I	CGCG	1 cut(s)	236
BshFI	GGCC	4 cut(s)	53, 72, 184, 223
BshNI	GGYRCC	2 cut(s)	113, 525
BshTI	ACCGGT	1 cut(s)	584
BshVI	ATCGAT	1 cut(s)	885
BsiHKCI	CYCGRG	1 cut(s)	185
BsiSI	CCGG	9 cut(s)	103, 155, 178, 186, 219, 224, 241, 585, 1102
BslFI	GGGAC	2 cut(s)	801, 1119
BsmAI	GTCTC	1 cut(s)	335
BsmBI	CGTCTC	1 cut(s)	335
BsmFI	GGGAC	2 cut(s)	801, 1119
BsnI	GGCC	4 cut(s)	53, 72, 184, 223
BsoBI	CYCGRG	1 cut(s)	185
Bsp120I	GGGCCC	1 cut(s)	182
Bsp1286I	GDGCHC	1 cut(s)	186
Bsp143I	GATC	3 cut(s)	763, 808, 1016
BspACI	CCGC	8 cut(s)	50, 61, 100, 127, 197, 236, 263, 925
BspANI	GGCC	4 cut(s)	53, 72, 184, 223
BspCNI	CTCAG	3 cut(s)	237, 696, 784
BspDI	ATCGAT	1 cut(s)	885
BspFNI	CGCG	1 cut(s)	236
BspLI	GGNNCC	5 cut(s)	115, 183, 184, 527, 1018
BspPI	GGATC	3 cut(s)	758, 1011, 1024
BspQI	GCTCTTC	2 cut(s)	595, 669
BspT107I	GGYRCC	2 cut(s)	113, 525
BsrFI	RCCGGY	4 cut(s)	154, 223, 240, 584
BsrI	ACTGG	1 cut(s)	789
BssAI	RCCGGY	4 cut(s)	154, 223, 240, 584
BssECI	CCNNGG	6 cut(s)	67, 178, 185, 529, 797, 1102
BssMI	GATC	3 cut(s)	763, 808, 1016
BssNI	GRCGYC	1 cut(s)	114
BssSI	CACGAG	1 cut(s)	696
BssT1I	CCWWGG	3 cut(s)	67, 529, 797
Bst2BI	CACGAG	1 cut(s)	696
Bst4CI	ACNGT	2 cut(s)	337, 1052
Bst6I	CTCTTC	2 cut(s)	595, 669
BstACI	GRCGYC	1 cut(s)	114
BstC8I	GCNNGC	5 cut(s)	127, 463, 467, 869, 1081
BstDEI	CTNAG	5 cut(s)	245, 704, 771, 913, 1070
BstEII	GGTNACC	1 cut(s)	226
BstF5I	GGATG	3 cut(s)	377, 857, 946
BstFNI	CGCG	1 cut(s)	236
BstH2I	RGCGCY	1 cut(s)	117
BstHHI	GCGC	2 cut(s)	116, 134
BstKTI	GATC	3 cut(s)	766, 811, 1019
BstMAI	GTCTC	1 cut(s)	335
BstMBI	GATC	3 cut(s)	763, 808, 1016
BstMWI	GCNNNNNNNGC	3 cut(s)	69, 122, 131
BstNSI	RCATGY	3 cut(s)	469, 830, 871
BstPAI	GACNNNNGTC	1 cut(s)	1050
BstPI	GGTNACC	1 cut(s)	226
BstSCI	CCNGG	5 cut(s)	177, 184, 185, 218, 1101
BstSLI	GKGCMC	1 cut(s)	186
BstUI	CGCG	1 cut(s)	236
BstV1I	GCAGC	2 cut(s)	116, 121
BstX2I	RGATCY	3 cut(s)	763, 808, 1016
BstYI	RGATCY	3 cut(s)	763, 808, 1016
Bsu15I	ATCGAT	1 cut(s)	885
BsuI	GTATCC	1 cut(s)	558
BsuRI	GGCC	4 cut(s)	53, 72, 184, 223
BsuTUI	ATCGAT	1 cut(s)	885
BtgZI	GCGATG	1 cut(s)	1098
BtsCI	GGATG	3 cut(s)	377, 857, 946
BtsIMutI	CAGTG	1 cut(s)	782
Cac8I	GCNNGC	5 cut(s)	127, 463, 467, 869, 1081
CfoI	GCGC	2 cut(s)	116, 134
Cfr10I	RCCGGY	4 cut(s)	154, 223, 240, 584
Cfr13I	GGNCC	8 cut(s)	31, 52, 71, 175, 182, 183, 221, 238
Cfr9I	CCCGGG	1 cut(s)	185
ClaI	ATCGAT	1 cut(s)	885
CpoI	CGGWCCG	2 cut(s)	175, 238
CspAI	ACCGGT	1 cut(s)	584
CspI	CGGWCCG	2 cut(s)	175, 238
CviAII	CATG	9 cut(s)	259, 380, 430, 466, 522, 817, 827, 868, 893
DdeI	CTNAG	5 cut(s)	245, 704, 771, 913, 1070
DinI	GGCGCC	1 cut(s)	115
DpnI	GATC	3 cut(s)	765, 810, 1018
DpnII	GATC	3 cut(s)	763, 808, 1016
DraIII	CACNNNGTG	1 cut(s)	46
DriI	GACNNNNNGTC	1 cut(s)	274
Eam1104I	CTCTTC	2 cut(s)	595, 669
Eam1105I	GACNNNNNGTC	1 cut(s)	274
EarI	CTCTTC	2 cut(s)	595, 669
EciI	GGCGGA	1 cut(s)	278
Eco130I	CCWWGG	3 cut(s)	67, 529, 797
Eco24I	GRGCYC	1 cut(s)	186
Eco47I	GGWCC	3 cut(s)	31, 175, 238
Eco88I	CYCGRG	1 cut(s)	185
Eco91I	GGTNACC	1 cut(s)	226
EcoO109I	RGGNCCY	1 cut(s)	182
EcoO65I	GGTNACC	1 cut(s)	226
EcoT14I	CCWWGG	3 cut(s)	67, 529, 797
EcoT22I	ATGCAT	2 cut(s)	444, 471
EcoT38I	GRGCYC	1 cut(s)	186
EgeI	GGCGCC	1 cut(s)	115
EheI	GGCGCC	1 cut(s)	115
ErhI	CCWWGG	3 cut(s)	67, 529, 797
Esp3I	CGTCTC	1 cut(s)	335
FaeI	CATG	9 cut(s)	262, 383, 433, 469, 525, 820, 830, 871, 896
FalI	AAGNNNNNCTT	2 cut(s)	275, 307
FaqI	GGGAC	2 cut(s)	801, 1119
FatI	CATG	9 cut(s)	258, 379, 429, 465, 521, 816, 826, 867, 892
FauI	CCCGC	4 cut(s)	107, 134, 190, 918
Fnu4HI	GCNGC	4 cut(s)	51, 61, 130, 135
FokI	GGATG	3 cut(s)	384, 864, 953
FriOI	GRGCYC	1 cut(s)	186
Fsp4HI	GCNGC	4 cut(s)	51, 61, 130, 135
FspBI	CTAG	4 cut(s)	552, 639, 860, 963
GlaI	GCGC	2 cut(s)	115, 133
GluI	GCNGC	4 cut(s)	51, 61, 130, 135
GsuI	CTGGAG	2 cut(s)	972, 1138
HaeII	RGCGCY	1 cut(s)	117
HaeIII	GGCC	4 cut(s)	53, 72, 184, 223
HapII	CCGG	9 cut(s)	103, 155, 178, 186, 219, 224, 241, 585, 1102
HhaI	GCGC	2 cut(s)	116, 134
Hin1I	GRCGYC	1 cut(s)	114
Hin1II	CATG	9 cut(s)	262, 383, 433, 469, 525, 820, 830, 871, 896
Hin6I	GCGC	2 cut(s)	114, 132
HinP1I	GCGC	2 cut(s)	114, 132
HincII	GTYRAC	1 cut(s)	935
HindII	GTYRAC	1 cut(s)	935
HpaII	CCGG	9 cut(s)	103, 155, 178, 186, 219, 224, 241, 585, 1102
HphI	GGTGA	6 cut(s)	132, 220, 599, 631, 649, 901
Hpy166II	GTNNAC	2 cut(s)	31, 935
Hpy188I	TCNGA	3 cut(s)	175, 851, 1093
Hpy188III	TCNNGA	8 cut(s)	13, 316, 340, 719, 812, 860, 875, 1117
Hpy8I	GTNNAC	2 cut(s)	31, 935
Hpy99I	CGWCG	3 cut(s)	170, 341, 610
HpyAV	CCTTC	2 cut(s)	153, 530
HpyCH4III	ACNGT	2 cut(s)	337, 1052
HpyCH4V	TGCA	8 cut(s)	361, 375, 442, 469, 901, 1028, 1063, 1133
HpyF10VI	GCNNNNNNNGC	3 cut(s)	69, 122, 131
HpyF3I	CTNAG	5 cut(s)	245, 704, 771, 913, 1070
Hsp92I	GRCGYC	1 cut(s)	114
Hsp92II	CATG	9 cut(s)	262, 383, 433, 469, 525, 820, 830, 871, 896
HspAI	GCGC	2 cut(s)	114, 132
KasI	GGCGCC	1 cut(s)	113
Kzo9I	GATC	3 cut(s)	763, 808, 1016
LguI	GCTCTTC	2 cut(s)	595, 669
LmnI	GCTCC	4 cut(s)	189, 255, 697, 991
Lsp1109I	GCAGC	2 cut(s)	116, 121
LweI	GCATC	4 cut(s)	362, 451, 973, 1072
MaeI	CTAG	4 cut(s)	552, 639, 860, 963
MaeIII	GTNAC	4 cut(s)	226, 454, 655, 1001
MalI	GATC	3 cut(s)	765, 810, 1018
MboI	GATC	3 cut(s)	763, 808, 1016
MboII	GAAGA	4 cut(s)	612, 686, 773, 1046
MflI	RGATCY	3 cut(s)	763, 808, 1016
MhlI	GDGCHC	1 cut(s)	186
MluCI	AATT	4 cut(s)	36, 90, 543, 952
Mly113I	GGCGCC	1 cut(s)	114
MnlI	CCTC	8 cut(s)	148, 240, 242, 259, 294, 304, 358, 882
Mph1103I	ATGCAT	2 cut(s)	444, 471
MseI	TTAA	3 cut(s)	321, 740, 831
MspA1I	CMGCKG	1 cut(s)	129
MspI	CCGG	9 cut(s)	103, 155, 178, 186, 219, 224, 241, 585, 1102
MspR9I	CCNGG	5 cut(s)	179, 186, 187, 220, 1103
MvnI	CGCG	1 cut(s)	236
MwoI	GCNNNNNNNGC	3 cut(s)	69, 122, 131
NarI	GGCGCC	1 cut(s)	114
NciI	CCSGG	5 cut(s)	179, 186, 187, 220, 1103
NdeII	GATC	3 cut(s)	763, 808, 1016
NlaIII	CATG	9 cut(s)	262, 383, 433, 469, 525, 820, 830, 871, 896
NlaIV	GGNNCC	5 cut(s)	115, 183, 184, 527, 1018
NmuCI	GTSAC	2 cut(s)	226, 655
NsiI	ATGCAT	2 cut(s)	444, 471
NspI	RCATGY	3 cut(s)	469, 830, 871
PaeI	GCATGC	2 cut(s)	469, 871
PciSI	GCTCTTC	2 cut(s)	595, 669
PflFI	GACNNNGTC	1 cut(s)	947
PinAI	ACCGGT	1 cut(s)	584
PkrI	GCNGC	4 cut(s)	52, 62, 131, 136
PluTI	GGCGCC	1 cut(s)	117
PshAI	GACNNNNGTC	1 cut(s)	1050
PspEI	GGTNACC	1 cut(s)	226
PspN4I	GGNNCC	5 cut(s)	115, 183, 184, 527, 1018
PspOMI	GGGCCC	1 cut(s)	182
PspPI	GGNCC	8 cut(s)	31, 52, 71, 175, 182, 183, 221, 238
PsuI	RGATCY	3 cut(s)	763, 808, 1016
PsyI	GACNNNGTC	1 cut(s)	947
Rsr2I	CGGWCCG	2 cut(s)	175, 238
RsrII	CGGWCCG	2 cut(s)	175, 238
SapI	GCTCTTC	2 cut(s)	595, 669
SaqAI	TTAA	3 cut(s)	321, 740, 831
SatI	GCNGC	4 cut(s)	51, 61, 130, 135
Sau3AI	GATC	3 cut(s)	763, 808, 1016
Sau96I	GGNCC	8 cut(s)	31, 52, 71, 175, 182, 183, 221, 238
ScrFI	CCNGG	5 cut(s)	179, 186, 187, 220, 1103
SduI	GDGCHC	1 cut(s)	186
SfaNI	GCATC	4 cut(s)	362, 451, 973, 1072
SfoI	GGCGCC	1 cut(s)	115
SgrAI	CRCCGGYG	1 cut(s)	584
SinI	GGWCC	3 cut(s)	31, 175, 238
SmaI	CCCGGG	1 cut(s)	187
SmlI	CTYRAG	3 cut(s)	384, 569, 873
SmoI	CTYRAG	3 cut(s)	384, 569, 873
SpeI	ACTAGT	1 cut(s)	962
SphI	GCATGC	2 cut(s)	469, 871
Sse9I	AATT	4 cut(s)	36, 90, 543, 952
SsiI	CCGC	8 cut(s)	50, 61, 100, 127, 197, 236, 263, 925
SspDI	GGCGCC	1 cut(s)	113
SspMI	CTAG	4 cut(s)	552, 639, 860, 963
StyD4I	CCNGG	5 cut(s)	177, 184, 185, 218, 1101
StyI	CCWWGG	3 cut(s)	67, 529, 797
TaaI	ACNGT	2 cut(s)	337, 1052
TaqI	TCGA	5 cut(s)	14, 207, 339, 813, 885
TasI	AATT	4 cut(s)	36, 90, 543, 952
TauI	GCSGC	2 cut(s)	53, 63
Tru1I	TTAA	3 cut(s)	321, 740, 831
Tru9I	TTAA	3 cut(s)	321, 740, 831
TscAI	CASTG	1 cut(s)	789
TseFI	GTSAC	2 cut(s)	226, 655
TseI	GCWGC	2 cut(s)	129, 134
Tsp45I	GTSAC	2 cut(s)	226, 655
TspDTI	ATGAA	3 cut(s)	641, 833, 909
TspGWI	ACGGA	2 cut(s)	423, 1020
TspMI	CCCGGG	1 cut(s)	185
TspRI	CASTG	1 cut(s)	789
Tth111I	GACNNNGTC	1 cut(s)	947
VpaK11BI	GGWCC	3 cut(s)	31, 175, 238
XapI	RAATTY	1 cut(s)	36
XbaI	TCTAGA	1 cut(s)	859
XceI	RCATGY	3 cut(s)	469, 830, 871
XmaI	CCCGGG	1 cut(s)	185
XspI	CTAG	4 cut(s)	552, 639, 860, 963
Zsp2I	ATGCAT	2 cut(s)	444, 471

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.