MD02G1130100.v1.1

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009234
Physical Location & Seq
Forward (+)
10486376 .. 10489053
2678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1130100.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGAAGCCACCGTCAAAGCTTCCTCTCTCCGGTGGAAAATTCTCCGCCAAGCTCTCCTCCGCAAACCCACCGGAGCAGATTCGCAATCCGAAATCAGCGTCAAGAAAATCACGAGGAAGACGACGCAGGGCTTCAACTTGATCCCATCCCACATCGCCAACGACCCATCGTCTCGCTCCGGCGACGTCACCGTCTGCTACACCTTGCCCGGCGACTCTGCCCCCAAGCTTCACCTCACTCAAAGAGTGGTTGACCGAGCTGAGCTCAGTGACTTTGAGATTTGTAATGAGTACAATATTGACAACACTGGGCTTGTTTGTCAGTGGCCGTCGGAAGAGGTTCTTGCGTATTTTTGCTTGACTCACGCGGACATGTTCAGGTCGAAAAGTGTACTTGAGCTTGGGTCAGGCTATGGCTTAGGCGGCTTGGTTATTGCAGCTGTTACTGAGGCATCAGAAGTTGTAATTTCAGATGGGAATCCGCAAGTAGTACATTATATTCAGCACAATATTGATGCAAACTCTAAGGCATTTGGTGGTACAAGAGTGAAGTCCATGATGTTGCATTGGAGTCAGAACGAAATTTCTACTATCTCTAGCGAATTTGATCTCATTGTTGCTAGTGACTGCACTTTTTTTAAGGAATTCCACAATGGACTTGCTCAAATTCTCAAATTTCTGCTGGGAAAAGTGCAATCCTCCGAAGCTATATTCTTCAGTCCTAAAAGGGGTGACTCCTTAGACAAGTTCTTAGAAACAATCAAAGAATGTGGTTTGCACTTCAGCATAACAGAGAATTATGATGCAGAAATTTGGAAGCATCACGAGGAGTTTTTGGACGGTGATGGTTCCTGGCCCAGCTATCAAAAGGATCACTGCTATCCCTTATTGATCAGGATTACAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

303

Amino Acids

33.74

Weight (kDa)

5.66

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 109 - 256 1e-22 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 189
AccII CGCG 1 cut(s) 368
AciI CCGC 5 cut(s) 46, 61, 368, 423, 482
AclWI GGATC 2 cut(s) 136, 879
AcoI YGGCCR 1 cut(s) 326
AcsI RAATTY 7 cut(s) 39, 582, 602, 644, 666, 674, 810
AcuI CTGAAG 2 cut(s) 700, 766
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 4 cut(s) 293, 393, 492, 541
AfiI CCNNNNNNNGG 2 cut(s) 30, 728
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 1 cut(s) 136
AhdI GACNNNNNGTC 1 cut(s) 169
AjnI CCWGG 1 cut(s) 851
AluBI AGCT 9 cut(s) 20, 53, 229, 260, 265, 400, 440, 707, 861
AluI AGCT 9 cut(s) 20, 53, 229, 260, 265, 400, 440, 707, 861
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 2 cut(s) 136, 879
AoxI GGCC 2 cut(s) 326, 854
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 7 cut(s) 39, 582, 602, 644, 666, 674, 810
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
Asp700I GAANNNNTTC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 855
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 4 cut(s) 181, 224, 743, 854
BaeI ACNNNNGTAYC 2 cut(s) 531, 564
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 2 cut(s) 112, 824
BbsI GAAGAC 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 449
BccI CCATC 4 cut(s) 154, 175, 467, 839
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 853
BclI TGATCA 1 cut(s) 891
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 2 cut(s) 597, 621
BisI GCNGC 2 cut(s) 424, 438
BlpI GCTNAGC 1 cut(s) 261
BlsI GCNGC 2 cut(s) 425, 439
Bme1390I CCNGG 2 cut(s) 210, 853
BmeRI GACNNNNNGTC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 855
BmiI GGNNCC 1 cut(s) 850
BmrFI CCNGG 2 cut(s) 210, 853
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 4 cut(s) 461, 505, 793, 829
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 125
BplI GAGNNNNNCTC 2 cut(s) 249, 281
Bpu10I CCTNAGC 1 cut(s) 418
Bpu1102I GCTNAGC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 186
BsaWI WCCGGW 2 cut(s) 30, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 728
Bse1I ACTGG 1 cut(s) 313
Bse8I GATNNNNATC 1 cut(s) 477
BseBI CCWGG 1 cut(s) 853
BseGI GGATG 1 cut(s) 146
BseJI GATNNNNATC 1 cut(s) 477
BseLI CCNNNNNNNGG 2 cut(s) 30, 728
BseMII CTCAG 3 cut(s) 252, 280, 438
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 2 cut(s) 47, 842
BseXI GCAGC 1 cut(s) 449
BseYI CCCAGC 2 cut(s) 682, 857
BsgI GTGCAG 1 cut(s) 613
Bsh1236I CGCG 1 cut(s) 368
BshFI GGCC 2 cut(s) 328, 856
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 4 cut(s) 31, 72, 180, 210
BslI CCNNNNNNNGG 2 cut(s) 30, 728
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
BsnI GGCC 2 cut(s) 328, 856
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 4 cut(s) 141, 607, 871, 891
Bsp1720I GCTNAGC 1 cut(s) 261
BspACI CCGC 5 cut(s) 46, 61, 368, 423, 482
BspANI GGCC 2 cut(s) 328, 856
BspCNI CTCAG 3 cut(s) 253, 279, 439
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 1 cut(s) 850
BspPI GGATC 2 cut(s) 136, 879
BsrI ACTGG 1 cut(s) 313
BssMI GATC 4 cut(s) 141, 607, 871, 891
BssNI GRCGYC 1 cut(s) 186
BssSI CACGAG 2 cut(s) 112, 824
Bst2BI CACGAG 2 cut(s) 112, 824
Bst2UI CCWGG 1 cut(s) 853
Bst4CI ACNGT 4 cut(s) 13, 193, 842, 904
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 186
BstDEI CTNAG 7 cut(s) 261, 266, 418, 447, 525, 739, 751
BstF5I GGATG 1 cut(s) 146
BstFNI CGCG 1 cut(s) 368
BstKTI GATC 4 cut(s) 144, 610, 874, 894
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 4 cut(s) 141, 607, 871, 891
BstMWI GCNNNNNNNGC 1 cut(s) 423
BstNI CCWGG 1 cut(s) 853
BstNSI RCATGY 1 cut(s) 376
BstSCI CCNGG 2 cut(s) 208, 851
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 1 cut(s) 449
BstV2I GAAGAC 1 cut(s) 125
BsuRI GGCC 2 cut(s) 328, 856
BtgZI GCGATG 1 cut(s) 139
BtsCI GGATG 1 cut(s) 146
BtsI GCAGTG 1 cut(s) 874
BtsIMutI CAGTG 4 cut(s) 274, 306, 329, 874
Cfr13I GGNCC 1 cut(s) 855
CseI GACGC 2 cut(s) 88, 133
Csp6I GTAC 4 cut(s) 292, 392, 491, 540
CspCI CAANNNNNGTGG 2 cut(s) 751, 786
CviAII CATG 2 cut(s) 373, 556
CviQI GTAC 4 cut(s) 292, 392, 491, 540
DdeI CTNAG 7 cut(s) 261, 266, 418, 447, 525, 739, 751
DpnI GATC 4 cut(s) 143, 609, 873, 893
DpnII GATC 4 cut(s) 141, 607, 871, 891
DrdI GACNNNNNNGTC 1 cut(s) 191
DriI GACNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 330
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 35
Ecl136II GAGCTC 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 700, 766
EcoICRI GAGCTC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 851
EcoT38I GRGCYC 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 2 cut(s) 376, 559
FaiI YATR 8 cut(s) 374, 414, 498, 557, 710, 788, 801, 907
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FatI CATG 2 cut(s) 372, 555
FbaI TGATCA 1 cut(s) 891
Fnu4HI GCNGC 2 cut(s) 424, 438
FokI GGATG 1 cut(s) 133
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 2 cut(s) 424, 438
FspBI CTAG 2 cut(s) 597, 621
GluI GCNGC 2 cut(s) 424, 438
GsaI CCCAGC 2 cut(s) 686, 861
HaeIII GGCC 2 cut(s) 328, 856
HapII CCGG 4 cut(s) 31, 72, 180, 210
HgaI GACGC 2 cut(s) 88, 133
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 2 cut(s) 376, 559
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 2 cut(s) 18, 227
HinfI GANTC 6 cut(s) 80, 215, 361, 478, 571, 734
HpaII CCGG 4 cut(s) 31, 72, 180, 210
HphI GGTGA 4 cut(s) 181, 224, 743, 854
Hpy166II GTNNAC 2 cut(s) 253, 392
Hpy188I TCNGA 6 cut(s) 91, 334, 457, 472, 576, 703
Hpy188III TCNNGA 4 cut(s) 103, 112, 824, 895
Hpy8I GTNNAC 2 cut(s) 253, 392
Hpy99I CGWCG 3 cut(s) 127, 188, 334
HpyCH4III ACNGT 4 cut(s) 13, 193, 842, 904
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 7 cut(s) 437, 518, 565, 630, 694, 778, 806
HpyF10VI GCNNNNNNNGC 1 cut(s) 423
HpyF3I CTNAG 7 cut(s) 261, 266, 418, 447, 525, 739, 751
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 2 cut(s) 376, 559
Ksp22I TGATCA 1 cut(s) 891
Kzo9I GATC 4 cut(s) 141, 607, 871, 891
LmnI GCTCC 2 cut(s) 74, 182
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 4 cut(s) 461, 505, 793, 829
MaeI CTAG 2 cut(s) 597, 621
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 5 cut(s) 187, 269, 442, 623, 731
MalI GATC 4 cut(s) 143, 609, 873, 893
MboI GATC 4 cut(s) 141, 607, 871, 891
MboII GAAGA 3 cut(s) 130, 347, 706
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 9 cut(s) 39, 465, 582, 602, 644, 666, 674, 796, 810
MlyI GAGTC 4 cut(s) 209, 355, 580, 728
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 8 cut(s) 34, 68, 108, 245, 331, 442, 709, 820
MroXI GAANNNNTTC 1 cut(s) 339
MseI TTAA 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 4 cut(s) 31, 72, 180, 210
MspR9I CCNGG 2 cut(s) 210, 853
MvaI CCWGG 1 cut(s) 853
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 1 cut(s) 423
NciI CCSGG 1 cut(s) 210
NdeII GATC 4 cut(s) 141, 607, 871, 891
NlaIII CATG 2 cut(s) 376, 559
NlaIV GGNNCC 1 cut(s) 850
NmuCI GTSAC 4 cut(s) 187, 269, 623, 731
NspI RCATGY 1 cut(s) 376
PciI ACATGT 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 80, 478
PkrI GCNGC 2 cut(s) 425, 439
PleI GAGTC 4 cut(s) 209, 355, 579, 728
PpsI GAGTC 4 cut(s) 209, 355, 579, 728
PscI ACATGT 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 851
PspFI CCCAGC 2 cut(s) 682, 857
PspGI CCWGG 1 cut(s) 851
PspN4I GGNNCC 1 cut(s) 850
PspPI GGNCC 1 cut(s) 855
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 4 cut(s) 293, 393, 492, 541
RsaNI GTAC 4 cut(s) 292, 392, 491, 540
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 1 cut(s) 639
SatI GCNGC 2 cut(s) 424, 438
Sau3AI GATC 4 cut(s) 141, 607, 871, 891
Sau96I GGNCC 1 cut(s) 855
SchI GAGTC 4 cut(s) 209, 355, 580, 728
ScrFI CCNGG 2 cut(s) 210, 853
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 4 cut(s) 461, 505, 793, 829
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 9 cut(s) 39, 465, 582, 602, 644, 666, 674, 796, 810
SsiI CCGC 5 cut(s) 46, 61, 368, 423, 482
SspI AATATT 2 cut(s) 298, 511
SspMI CTAG 2 cut(s) 597, 621
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 2 cut(s) 208, 851
TaaI ACNGT 4 cut(s) 13, 193, 842, 904
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 383
TaqII GACCGA 1 cut(s) 270
TasI AATT 9 cut(s) 39, 465, 582, 602, 644, 666, 674, 796, 810
TatI WGTACW 3 cut(s) 291, 391, 490
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 2 cut(s) 80, 478
Tru1I TTAA 1 cut(s) 639
Tru9I TTAA 1 cut(s) 639
TscAI CASTG 4 cut(s) 274, 313, 329, 881
TseFI GTSAC 4 cut(s) 187, 269, 623, 731
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 4 cut(s) 187, 269, 623, 731
TspRI CASTG 4 cut(s) 274, 313, 329, 881
XapI RAATTY 7 cut(s) 39, 582, 602, 644, 666, 674, 810
XceI RCATGY 1 cut(s) 376
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 2 cut(s) 597, 621
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.