RLG00000016868

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009234
Physical Location & Seq
Forward (+)
10789252 .. 10791184
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016868

Sequence Viewer

Length: 660 bp
ATGGAAACCGGAGGCCCAACCACGGCCAAACCCTCGTCTCTCCGATGGAAAATTCTCCGTCAAGCCCTTCTTCGCAAACCAACCGCAGATGAGCAATCTGCAATTGGGGTCAAGCGAATCACGAGGAAGACGACTCAAGGCTTCAACTTGATACCCTCTAATCTCGTTGAAGACCACCCAACTAACTCAACCGACGCAACTGTCTGCTACACCATCCCCATTGACTCTGCTCCCAAGCTTTACCTAACTCAACGAGTGATTGACCGTGCTGAGCTCAGTGACTTTGAGATTTGCAATCAGTACAATATTGACAATACTGGGCTTGTTTGTCAGTGGCCATCAGAAGAGGCTCTTGCATATTTTTGCTTGCGGAATGCAGACATGTTCAGGTCTAAGAGTGTGATTGAGCTTGGGGCAGGATATGGATTAGCTGGTCTAGTTATTGCAGCAGTTACAGAGGCATCGGAAGTTGTGATTTCTGACGGAAATCCCCAAGTAGTTGATTATATTCAGCGCAATATTGATGCCAACTCAAAAGCATTTGGTGCTACAAGAGTGAAGTCTATGATGTTGCATTGGAATCAGAATGAAAAATCAAATATCTCTAGCACTTTTGATCTCATTGTTGCGAGTGACTGGTATGTTCTTCCATATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

220

Amino Acids

24.33

Weight (kDa)

5.46

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 112 - 212 2.9e-16 Lysine methyltransferase
MTS PF05175 128 - 189 5.2e-06 Methyltransferase small domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
AciI CCGC 2 cut(s) 84, 370
AcoI YGGCCR 2 cut(s) 24, 335
AcsI RAATTY 1 cut(s) 51
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 1 cut(s) 22
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 2 cut(s) 145, 170
AluBI AGCT 4 cut(s) 238, 274, 409, 431
AluI AGCT 4 cut(s) 238, 274, 409, 431
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 1 cut(s) 42
AoxI GGCC 3 cut(s) 13, 24, 335
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 51
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 14
BalI TGGCCA 1 cut(s) 337
BanII GRGCYC 1 cut(s) 276
BauI CACGAG 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 134, 177
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 458
BccI CCATC 3 cut(s) 39, 221, 346
BceAI ACGGC 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 42
BfaI CTAG 2 cut(s) 437, 606
BisI GCNGC 1 cut(s) 447
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 14
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 2 cut(s) 470, 514
BmuI ACTGGG 1 cut(s) 327
BpiI GAAGAC 2 cut(s) 134, 177
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 2 cut(s) 322, 641
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMII CTCAG 2 cut(s) 261, 289
BseNI ACTGG 2 cut(s) 322, 641
BseXI GCAGC 1 cut(s) 458
BshFI GGCC 3 cut(s) 15, 26, 337
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 42
BsmBI CGTCTC 1 cut(s) 42
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 3 cut(s) 15, 26, 337
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 1 cut(s) 616
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 2 cut(s) 84, 370
BspANI GGCC 3 cut(s) 15, 26, 337
BspCNI CTCAG 2 cut(s) 262, 288
BsrI ACTGG 2 cut(s) 322, 641
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 1 cut(s) 616
BssSI CACGAG 1 cut(s) 121
Bst2BI CACGAG 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 202, 266
Bst6I CTCTTC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 3 cut(s) 270, 275, 393
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 516
BstKTI GATC 1 cut(s) 619
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 1 cut(s) 616
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNSI RCATGY 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 458
BstV2I GAAGAC 2 cut(s) 134, 177
BsuRI GGCC 3 cut(s) 15, 26, 337
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 283, 338
Cac8I GCNNGC 1 cut(s) 368
CfoI GCGC 1 cut(s) 516
Cfr13I GGNCC 1 cut(s) 14
CseI GACGC 1 cut(s) 203
Csp6I GTAC 1 cut(s) 301
CviAII CATG 1 cut(s) 382
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 3 cut(s) 270, 275, 393
DpnI GATC 1 cut(s) 618
DpnII GATC 1 cut(s) 616
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
EaeI YGGCCR 2 cut(s) 24, 335
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 274
EcoICRI GAGCTC 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 1 cut(s) 385
FaiI YATR 7 cut(s) 358, 383, 423, 507, 566, 642, 652
FalI AAGNNNNNCTT 4 cut(s) 54, 86, 336, 368
FatI CATG 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 2 cut(s) 437, 606
GlaI GCGC 1 cut(s) 515
GluI GCNGC 1 cut(s) 447
HaeIII GGCC 3 cut(s) 15, 26, 337
HapII CCGG 1 cut(s) 9
HgaI GACGC 1 cut(s) 203
HhaI GCGC 1 cut(s) 516
Hin1II CATG 1 cut(s) 385
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 4 cut(s) 117, 133, 224, 580
HpaII CCGG 1 cut(s) 9
Hpy188I TCNGA 6 cut(s) 44, 343, 466, 481, 585, 659
Hpy188III TCNNGA 1 cut(s) 121
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 2 cut(s) 202, 266
HpyCH4V TGCA 6 cut(s) 101, 294, 356, 377, 446, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 3 cut(s) 270, 275, 393
Hsp92II CATG 1 cut(s) 385
HspAI GCGC 1 cut(s) 514
Kzo9I GATC 1 cut(s) 616
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 6 cut(s) 22, 303, 373, 402, 417, 622
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 2 cut(s) 470, 514
MaeI CTAG 2 cut(s) 437, 606
MaeIII GTNAC 3 cut(s) 278, 451, 632
MalI GATC 1 cut(s) 618
MboI GATC 1 cut(s) 616
MboII GAAGA 5 cut(s) 62, 139, 182, 356, 638
MfeI CAATTG 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 276
MlsI TGGCCA 1 cut(s) 337
MluCI AATT 2 cut(s) 51, 102
MluNI TGGCCA 1 cut(s) 337
MlyI GAGTC 2 cut(s) 127, 218
MnlI CCTC 6 cut(s) 5, 43, 117, 166, 340, 451
Mox20I TGGCCA 1 cut(s) 337
MscI TGGCCA 1 cut(s) 337
Msp20I TGGCCA 1 cut(s) 337
MspI CCGG 1 cut(s) 9
MunI CAATTG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeII GATC 1 cut(s) 616
NlaIII CATG 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 278, 632
NspI RCATGY 1 cut(s) 385
PciI ACATGT 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 117, 580
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 2 cut(s) 127, 218
PpsI GAGTC 2 cut(s) 127, 218
PscI ACATGT 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 276
PspPI GGNCC 1 cut(s) 14
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SacI GAGCTC 1 cut(s) 276
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 1 cut(s) 616
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 2 cut(s) 127, 218
SduI GDGCHC 1 cut(s) 276
SetI ASST 6 cut(s) 240, 246, 276, 392, 411, 433
SfaNI GCATC 2 cut(s) 470, 514
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
Sse9I AATT 2 cut(s) 51, 102
SsiI CCGC 2 cut(s) 84, 370
SspI AATATT 2 cut(s) 307, 520
SspMI CTAG 2 cut(s) 437, 606
SstI GAGCTC 1 cut(s) 276
TaaI ACNGT 2 cut(s) 202, 266
TasI AATT 2 cut(s) 51, 102
TatI WGTACW 1 cut(s) 300
TfiI GAWTC 2 cut(s) 117, 580
TscAI CASTG 2 cut(s) 283, 338
TseFI GTSAC 2 cut(s) 278, 632
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 2 cut(s) 278, 632
TspDTI ATGAA 1 cut(s) 603
TspGWI ACGGA 2 cut(s) 47, 498
TspRI CASTG 2 cut(s) 283, 338
XapI RAATTY 1 cut(s) 51
XceI RCATGY 1 cut(s) 385
XspI CTAG 2 cut(s) 437, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.