MD02G1134300.v1.1

negative regulation of mRNA metabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011510
Physical Location & Seq
Forward (+)
10880436 .. 10885375
4940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1134300.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGAGACCTCGCAAGGCAGACGCCTCCAAACCGCCGACCGCTAAGAAAACGCCGCCGGCGAGGAAAACCGCCACCAGGCCACAGCCGGCGGCTTACCCGCCGGACTTAGAAGTGGCCACTCCGAAAACCGTCGAAACAAAGCGCGCTTGTGCCGGCCGGGTGAAGCAGGCCAAGAAGAACGAAACGACACCGCCTGCTGATTCGAAGTCCCCCAAACCATCAGCTGAGGGGACTGAGGGATCAGCTGGTACTGCTAAGGTGGCACCGGCGGCGAAAGTTGGTGTGGCGAGAAAAGTACCTGCGAAAAGGTCGCCGGTGAAACTTAAGACATCAGCTAAATCTGTTTCACCTCTGACACCGAAATTGGGGGGATCGGGGAAGGGGGAACTAGATGCAGTGAACAAGGGGCAGGCGGCAGATGGTAGTGATGTGGAAGTCACAGAGAAGAAACCGGTTGCTGAGAATGTTGGGGAGTCTGCAAAGAAGGGCGAAAGCGTTGTGGAAGTTGAAGTAAGCACTGTTGAGAATGTTGGAGAGCCTGCAACAATCAAACAAACAATCGTTGAAGTAAAAGTAAACCCAAATGAAGTTGAAGAGGATCCCGAAGAGGAAGAGGATCCTGTTGAGGAGGAGGATCCTATCGAGGAAGAGGATTCCGTTGAGGCAGTAGAATCAGTAGCTGGGGAGATTTCAGAGTCTTCTGAGATAGCTTGTGATATAAAAGAGGGAGTGGAAGTGATTGAGGAAGAGGGTCCTATCAAGGAAGAGGATCCCATTGAGGCAGAAAAATCTTTAGTTGCGGAGGTTTCAAAGTATTCTGAGAACGAACCAGCTCGTGATGTAAAAGAGGGAGTGGAAGTGAAAGAAGACCATAAGGAGAAGTCGGATAGAGTAGATGTGAAAGGAGATCAGGAGGAGTCAGTGGATGATGGAGATGAAGTCGAGACCTGTGTTGAGGCTACTAACAAGGTAGAAAATGCCAAAGAGGGGTTACAAGGAGAAGATATTGAGCGTGCTAAAGAGGTGTTTAATGGTGATGAGCAGATGGAAGATTATGGTGAAAAATTGGATCTGGGAGAACATGGAGAGGAGGAACTTCCTGAGGATGATGCAGAGGATCCTGCAGAAGAAACTGAGAGATTGGCTGATGAACATAAGGAATTTACAGCCATTGCAAATGAGCGCAAGATGAGGAAAGAACGTGAGATATTTCTTGGTGGGCTTGATCGGGATGCTGTGGAGGAAGATGTGAGAAGGGTCTTCGACAGGATTGGAGGGATAGTTGAAGTTAGGTTACACAAGAATCCTTCATCTAATAAGAACAAGGGTTATGCATTTGTGGAGTTTGAAAATAAGGAGTATGCAAGGCGGGCTTTGTCTGAAATGAAAAACCCTGTTATACGGGGGAGGCGATGTGGGACAGCACCTAGTGAGGACAATGACACGTTGTTTGTGGGTAATATATGCAATACCTGGACAAAGGAAGCTATTAAACAGAAACTGAAGGATTATGGTGTGGAAGGTGTTGAGAATATAAATCTTGTCCCAGATGTTCAAAGTGATGGGTTGAGCCGTGGTTTTGCATTTATTGAATTCTCTTGTCATGGTGAAGCTATGCTTGCATACAAGAGGCTTCAGCAGCCCGATGCTATATTTGGCCACACTGAAAGAACTGCCAAAGTGGCTTTTGCTGAACCTATACGTGAGCCTGACCCAGAGATCATGTCCCAAGTGAAGTCCGTATTTGTGGATGGGCTTCCACCTCATTGGGATGAGGACAAAGTTAGAGAGCAATTTAAATGCTATGGAGAAATCTTACGCGTTGTTCTGGCTCGGAATATGTCTACAGCCAAGCGGAGGGATTTTGGATTTGTTGATTTCTCTACCCATGAATCTGCTGTGGCCTTTGTTGATAGTATAAATAACATAGGACTGGAGGATGGGAATTCAAAGGTAAAAGTGAAAGCAAGACTTTCAAATCCTTTGCCTAAAACTCAGGCTGTGAAGGGTGGAATGTGCGGTGGATTTCGAATTGGTCGTGGTGGTGGTAGTGGTATCTTTCCAAGATCTGGACGGGGTTTTGGTCGTGGTGGAGATCATTTCAACCGTGCAAATTTCCAACCCAACAGACATTTCTATCGTGGTGGACGTGGTCAAACTGGCAGGATGGGTTTCCAGAATGAATATGACTTCGATTACCCATACGAGTTTCATGGACGAGGGGGAAGAAGGGGCTCTTTCAGGGGTGGCCGTTATGGATTTGATGGAGGTGCTCCAGTTGCTGCCCCACCATCAAGACCCTACACAAATCGACCTTGGCATGATGCCCCTGATAGAGGCTATGGTATGCATATTCCTCCTAGGATGCATCCATATTCTCTAGGTCCAGGACACCTTGATGGACCATTCATGGGGGTTCACTTTGATGATCCTTATTTTTATGATGATAACACACATGGAATAAAACGCCCATTTCATATGAGAGACTATGATCATGACTACTCGGAGCCTAGTAGTCGTCGCCCACGGTTGGATTACACTGATCCAGCAGCTTCGTTTCGAGGAAACCATTATAGGGATACGTATGGAGCTGGCAGCAGTGCCTACCCTCATGATTATTATGGTCCCGAATATAATTCTGGTCCATATTCATCTTATTACGGGAGCAATTACTCATATGGAGGTGGCCGCTACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0006417 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008143 GO:0008150 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016020 GO:0017148 GO:0019219 GO:0019222 GO:0030425 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032270 GO:0032991 GO:0034097 GO:0034248 GO:0034249 GO:0034250 GO:0034341 GO:0036477 GO:0042221 GO:0042995 GO:0043005 GO:0043025 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0043489 GO:0044297 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045087 GO:0045727 GO:0045934 GO:0048027 GO:0048255 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0070717 GO:0070887 GO:0070934 GO:0070937 GO:0071013 GO:0071204 GO:0071310 GO:0071345 GO:0071346 GO:0080090 GO:0090365 GO:0090367 GO:0097159 GO:0097447 GO:0097452 GO:0097458 GO:0120025 GO:0120038 GO:1901363 GO:1902369 GO:1902373 GO:1902494 GO:1903311 GO:1903312 GO:1990635 GO:1990904 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

900

Amino Acids

99.37

Weight (kDa)

5.15

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 403 - 471 3.7e-16 RNA recognition motif
RRM_1 PF00076 483 - 547 1.3e-07 RNA recognition motif
RRM_1 PF00076 581 - 645 5.2e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 307
AccB1I GGYRCC 1 cut(s) 262
AccB7I CCANNNNNTGG 1 cut(s) 2069
AccI GTMKAC 1 cut(s) 1844
AccII CGCG 2 cut(s) 144, 1821
AcoI YGGCCR 5 cut(s) 114, 154, 1657, 2248, 2686
AcsI RAATTY 4 cut(s) 1160, 1592, 1945, 2113
AcuI CTGAAG 2 cut(s) 1523, 1619
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 2 cut(s) 250, 297
AfiI CCNNNNNNNGG 9 cut(s) 75, 365, 987, 1857, 2069, 2336, 2411, 2530, 2575
AflII CTTAAG 1 cut(s) 323
AflIII ACRYGT 2 cut(s) 1443, 1819
AgeI ACCGGT 1 cut(s) 451
AjiI CACGTC 1 cut(s) 2150
AjnI CCWGG 3 cut(s) 74, 1472, 2386
AjuI GAANNNNNNNTTGG 2 cut(s) 2117, 2149
Alw21I GWGCWC 1 cut(s) 2275
Alw26I GTCTC 2 cut(s) 938, 2478
AlwNI CAGNNNCTG 3 cut(s) 680, 1501, 2282
AoxI GGCC 8 cut(s) 77, 114, 154, 168, 1657, 1902, 2248, 2686
ApeKI GCWGC 4 cut(s) 1639, 2282, 2549, 2595
ApoI RAATTY 4 cut(s) 1160, 1592, 1945, 2113
ArsI GACNNNNNNTTYG 2 cut(s) 1433, 1465
AsiGI ACCGGT 1 cut(s) 451
AspA2I CCTAGG 1 cut(s) 2360
AspLEI GCGC 3 cut(s) 144, 146, 1185
AspS9I GGNCC 5 cut(s) 752, 2384, 2402, 2624, 2642
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 6 cut(s) 172, 328, 339, 1046, 1070, 1619
AsuII TTCGAA 2 cut(s) 203, 2029
AvaII GGWCC 5 cut(s) 752, 2384, 2402, 2624, 2642
AvrII CCTAGG 1 cut(s) 2360
AxyI CCTNAGG 1 cut(s) 1101
BalI TGGCCA 2 cut(s) 116, 1659
BamHI GGATCC 5 cut(s) 598, 616, 634, 769, 1117
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 2237
BarI GAAGNNNNNNTAC 2 cut(s) 1278, 1310
BauI CACGAG 1 cut(s) 834
BbsI GAAGAC 3 cut(s) 690, 873, 1252
Bbv12I GWGCWC 1 cut(s) 2275
BbvCI CCTCAGC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 1651, 2269, 2561, 2607
BceAI ACGGC 2 cut(s) 1557, 2235
BciT130I CCWGG 3 cut(s) 76, 1474, 2388
BciVI GTATCC 1 cut(s) 2572
BclI TGATCA 1 cut(s) 2491
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 2 cut(s) 938, 2478
BfaI CTAG 5 cut(s) 389, 1428, 2361, 2381, 2511
BfmI CTRYAG 2 cut(s) 1122, 1845
BfrI CTTAAG 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 307
BfuI GTATCC 1 cut(s) 2572
BglI GCCNNNNNGGC 1 cut(s) 1682
BglII AGATCT 1 cut(s) 2066
BisI GCNGC 9 cut(s) 53, 90, 270, 414, 1640, 2283, 2550, 2596, 2689
BlnI CCTAGG 1 cut(s) 2360
BlsI GCNGC 9 cut(s) 54, 91, 271, 415, 1641, 2284, 2551, 2597, 2690
Bme1390I CCNGG 4 cut(s) 76, 158, 1474, 2388
Bme18I GGWCC 5 cut(s) 752, 2384, 2402, 2624, 2642
BmgBI CACGTC 1 cut(s) 2150
BmgT120I GGNCC 5 cut(s) 752, 2384, 2402, 2624, 2642
BmiI GGNNCC 9 cut(s) 264, 600, 618, 636, 753, 771, 1119, 2508, 2626
BmrFI CCNGG 4 cut(s) 76, 158, 1474, 2388
BmsI GCATC 7 cut(s) 382, 1099, 1222, 1636, 2314, 2355, 2377
BpiI GAAGAC 3 cut(s) 690, 873, 1252
BpmI CTGGAG 2 cut(s) 1955, 2259
Bpu10I CCTNAGC 2 cut(s) 225, 255
Bpu14I TTCGAA 2 cut(s) 203, 2029
BpuMI CCSGG 1 cut(s) 158
BsaAI YACGTR 2 cut(s) 1703, 2583
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 4 cut(s) 1573, 2315, 2360, 2525
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 9 cut(s) 75, 365, 987, 1857, 2069, 2336, 2411, 2530, 2575
Bse118I RCCGGY 6 cut(s) 55, 85, 152, 265, 313, 451
Bse1I ACTGG 3 cut(s) 1938, 2164, 2276
Bse21I CCTNAGG 1 cut(s) 1101
Bse3DI GCAATG 1 cut(s) 1170
BseBI CCWGG 3 cut(s) 76, 1474, 2388
BseDI CCNNGG 4 cut(s) 1573, 2315, 2360, 2525
BseGI GGATG 9 cut(s) 931, 1111, 1237, 1756, 1777, 1945, 2172, 2368, 2370
BseLI CCNNNNNNNGG 9 cut(s) 75, 365, 987, 1857, 2069, 2336, 2411, 2530, 2575
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 8 cut(s) 216, 225, 450, 693, 810, 1092, 1125, 2009
BseNI ACTGG 3 cut(s) 1938, 2164, 2276
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 4 cut(s) 641, 644, 929, 1103
BseX3I CGGCCG 1 cut(s) 154
BseXI GCAGC 4 cut(s) 1651, 2269, 2561, 2607
BseYI CCCAGC 1 cut(s) 680
Bsh1236I CGCG 2 cut(s) 144, 1821
Bsh1285I CGRYCG 2 cut(s) 39, 157
BshFI GGCC 8 cut(s) 79, 116, 156, 170, 1659, 1904, 2250, 2688
BshNI GGYRCC 1 cut(s) 262
BshTI ACCGGT 1 cut(s) 451
BsiEI CGRYCG 2 cut(s) 39, 157
BsiHKAI GWGCWC 1 cut(s) 2275
BsiSI CCGG 8 cut(s) 56, 86, 101, 153, 157, 266, 314, 452
BslFI GGGAC 6 cut(s) 193, 244, 1432, 1529, 1711, 2610
BslI CCNNNNNNNGG 9 cut(s) 75, 365, 987, 1857, 2069, 2336, 2411, 2530, 2575
BsmAI GTCTC 2 cut(s) 938, 2478
BsmFI GGGAC 6 cut(s) 193, 244, 1432, 1529, 1711, 2610
BsnI GGCC 8 cut(s) 79, 116, 156, 170, 1659, 1904, 2250, 2688
Bso31I GGTCTC 1 cut(s) 938
Bsp119I TTCGAA 2 cut(s) 203, 2029
Bsp1286I GDGCHC 2 cut(s) 2237, 2275
BspANI GGCC 8 cut(s) 79, 116, 156, 170, 1659, 1904, 2250, 2688
BspCNI CTCAG 8 cut(s) 217, 226, 451, 694, 811, 1093, 1126, 2008
BspFNI CGCG 2 cut(s) 144, 1821
BspHI TCATGA 2 cut(s) 2494, 2611
BspLI GGNNCC 9 cut(s) 264, 600, 618, 636, 753, 771, 1119, 2508, 2626
BspMAI CTGCAG 1 cut(s) 1126
BspMI ACCTGC 1 cut(s) 307
BspT104I TTCGAA 2 cut(s) 203, 2029
BspT107I GGYRCC 1 cut(s) 262
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 938
BsrDI GCAATG 1 cut(s) 1170
BsrFI RCCGGY 6 cut(s) 55, 85, 152, 265, 313, 451
BsrI ACTGG 3 cut(s) 1938, 2164, 2276
BssAI RCCGGY 6 cut(s) 55, 85, 152, 265, 313, 451
BssECI CCNNGG 4 cut(s) 1573, 2315, 2360, 2525
BssHII GCGCGC 1 cut(s) 142
BssNI GRCGYC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 2 cut(s) 2315, 2360
Bst2BI CACGAG 1 cut(s) 834
Bst2UI CCWGG 3 cut(s) 76, 1474, 2388
Bst4CI ACNGT 5 cut(s) 130, 520, 2108, 2529, 2696
Bst6I CTCTTC 6 cut(s) 588, 600, 606, 642, 741, 759
BstACI GRCGYC 1 cut(s) 21
BstAFI CTTAAG 1 cut(s) 323
BstBAI YACGTR 2 cut(s) 1703, 2583
BstBI TTCGAA 2 cut(s) 203, 2029
BstDSI CCRYGG 2 cut(s) 1573, 2525
BstENI CCTNNNNNAGG 1 cut(s) 2334
BstF5I GGATG 9 cut(s) 931, 1111, 1237, 1756, 1777, 1945, 2172, 2368, 2370
BstFNI CGCG 2 cut(s) 144, 1821
BstHHI GCGC 3 cut(s) 144, 146, 1185
BstMAI GTCTC 2 cut(s) 938, 2478
BstMCI CGRYCG 2 cut(s) 39, 157
BstMWI GCNNNNNNNGC 8 cut(s) 251, 260, 269, 1370, 1619, 1639, 1682, 2279
BstNI CCWGG 3 cut(s) 76, 1474, 2388
BstSCI CCNGG 4 cut(s) 74, 156, 1472, 2386
BstSFI CTRYAG 2 cut(s) 1122, 1845
BstSNI TACGTA 1 cut(s) 2583
BstUI CGCG 2 cut(s) 144, 1821
BstV1I GCAGC 4 cut(s) 1651, 2269, 2561, 2607
BstV2I GAAGAC 3 cut(s) 690, 873, 1252
BstX2I RGATCY 7 cut(s) 598, 616, 634, 769, 1069, 1117, 2066
BstXI CCANNNNNNTGG 1 cut(s) 1767
BstYI RGATCY 7 cut(s) 598, 616, 634, 769, 1069, 1117, 2066
BstZI CGGCCG 1 cut(s) 154
Bsu36I CCTNAGG 1 cut(s) 1101
BsuI GTATCC 1 cut(s) 2572
BsuRI GGCC 8 cut(s) 79, 116, 156, 170, 1659, 1904, 2250, 2688
BtgI CCRYGG 2 cut(s) 1573, 2525
BtgZI GCGATG 1 cut(s) 1426
BtrI CACGTC 1 cut(s) 2150
BtsCI GGATG 9 cut(s) 931, 1111, 1237, 1756, 1777, 1945, 2172, 2368, 2370
BtsI GCAGTG 2 cut(s) 402, 2605
BtsIMutI CAGTG 6 cut(s) 402, 516, 927, 1662, 2538, 2605
BveI ACCTGC 1 cut(s) 307
CaiI CAGNNNCTG 3 cut(s) 680, 1501, 2282
CciI TCATGA 2 cut(s) 2494, 2611
CfoI GCGC 3 cut(s) 144, 146, 1185
Cfr10I RCCGGY 6 cut(s) 55, 85, 152, 265, 313, 451
Cfr13I GGNCC 5 cut(s) 752, 2384, 2402, 2624, 2642
CseI GACGC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 249, 296
CspAI ACCGGT 1 cut(s) 451
CviQI GTAC 2 cut(s) 249, 296
DraI TTTAAA 1 cut(s) 1798
DraIII CACNNNGTG 1 cut(s) 1430
EaeI YGGCCR 5 cut(s) 114, 154, 1657, 2248, 2686
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 6 cut(s) 588, 600, 606, 642, 741, 759
EarI CTCTTC 6 cut(s) 588, 600, 606, 642, 741, 759
EclXI CGGCCG 1 cut(s) 154
Eco105I TACGTA 1 cut(s) 2583
Eco130I CCWWGG 2 cut(s) 2315, 2360
Eco24I GRGCYC 1 cut(s) 2237
Eco31I GGTCTC 1 cut(s) 938
Eco47I GGWCC 5 cut(s) 752, 2384, 2402, 2624, 2642
Eco52I CGGCCG 1 cut(s) 154
Eco57I CTGAAG 2 cut(s) 1523, 1619
Eco81I CCTNAGG 1 cut(s) 1101
EcoNI CCTNNNNNAGG 1 cut(s) 2334
EcoO109I RGGNCCY 1 cut(s) 752
EcoRI GAATTC 2 cut(s) 1592, 1945
EcoRII CCWGG 3 cut(s) 74, 1472, 2386
EcoT14I CCWWGG 2 cut(s) 2315, 2360
EcoT22I ATGCAT 3 cut(s) 1336, 2352, 2370
EcoT38I GRGCYC 1 cut(s) 2237
ErhI CCWWGG 2 cut(s) 2315, 2360
FalI AAGNNNNNCTT 8 cut(s) 1357, 1389, 1602, 1634, 1956, 1988, 2221, 2253
FaqI GGGAC 6 cut(s) 193, 244, 1432, 1529, 1711, 2610
FauI CCCGC 2 cut(s) 105, 1362
FauNDI CATATG 2 cut(s) 2478, 2677
FbaI TGATCA 1 cut(s) 2491
FblI GTMKAC 1 cut(s) 1844
Fnu4HI GCNGC 9 cut(s) 53, 90, 270, 414, 1640, 2283, 2550, 2596, 2689
FokI GGATG 9 cut(s) 938, 1118, 1244, 1763, 1784, 1952, 2179, 2355, 2377
FriOI GRGCYC 1 cut(s) 2237
Fsp4HI GCNGC 9 cut(s) 53, 90, 270, 414, 1640, 2283, 2550, 2596, 2689
FspBI CTAG 5 cut(s) 389, 1428, 2361, 2381, 2511
GlaI GCGC 3 cut(s) 143, 145, 1184
GluI GCNGC 9 cut(s) 53, 90, 270, 414, 1640, 2283, 2550, 2596, 2689
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 2 cut(s) 1955, 2259
HaeIII GGCC 8 cut(s) 79, 116, 156, 170, 1659, 1904, 2250, 2688
HapII CCGG 8 cut(s) 56, 86, 101, 153, 157, 266, 314, 452
HgaI GACGC 1 cut(s) 29
HhaI GCGC 3 cut(s) 144, 146, 1185
Hin1I GRCGYC 1 cut(s) 21
Hin6I GCGC 3 cut(s) 142, 144, 1183
HinP1I GCGC 3 cut(s) 142, 144, 1183
HinfI GANTC 8 cut(s) 200, 473, 653, 671, 695, 917, 1303, 1892
HpaII CCGG 8 cut(s) 56, 86, 101, 153, 157, 266, 314, 452
HphI GGTGA 6 cut(s) 172, 328, 339, 1046, 1070, 1619
Hpy166II GTNNAC 5 cut(s) 400, 577, 1845, 2147, 2419
Hpy188I TCNGA 9 cut(s) 123, 354, 694, 703, 820, 886, 1381, 1836, 2506
Hpy8I GTNNAC 5 cut(s) 400, 577, 1845, 2147, 2419
Hpy99I CGWCG 2 cut(s) 134, 2523
HpyAV CCTTC 8 cut(s) 373, 478, 1248, 1317, 1498, 1514, 1999, 2223
HpyCH4III ACNGT 5 cut(s) 130, 520, 2108, 2529, 2696
HpyCH4IV ACGT 5 cut(s) 1201, 1445, 1702, 2149, 2582
HpyF10VI GCNNNNNNNGC 8 cut(s) 251, 260, 269, 1370, 1619, 1639, 1682, 2279
HpySE526I ACGT 5 cut(s) 1201, 1445, 1702, 2149, 2582
Hsp92I GRCGYC 1 cut(s) 21
HspAI GCGC 3 cut(s) 142, 144, 1183
KroI GCCGGC 3 cut(s) 55, 85, 152
KroNI GCCGGC 3 cut(s) 57, 87, 154
Ksp22I TGATCA 1 cut(s) 2491
LmnI GCTCC 4 cut(s) 2278, 2506, 2588, 2664
Lsp1109I GCAGC 4 cut(s) 1651, 2269, 2561, 2607
LweI GCATC 7 cut(s) 382, 1099, 1222, 1636, 2314, 2355, 2377
MaeI CTAG 5 cut(s) 389, 1428, 2361, 2381, 2511
MaeII ACGT 5 cut(s) 1201, 1445, 1702, 2149, 2582
MaeIII GTNAC 3 cut(s) 436, 990, 1293
MflI RGATCY 7 cut(s) 598, 616, 634, 769, 1069, 1117, 2066
MhlI GDGCHC 2 cut(s) 2237, 2275
MlsI TGGCCA 2 cut(s) 116, 1659
MluI ACGCGT 1 cut(s) 1819
MluNI TGGCCA 2 cut(s) 116, 1659
MlyI GAGTC 3 cut(s) 482, 704, 926
MmeI TCCRAC 4 cut(s) 511, 864, 2143, 2511
Mox20I TGGCCA 2 cut(s) 116, 1659
Mph1103I ATGCAT 3 cut(s) 1336, 2352, 2370
MreI CGCCGGCG 1 cut(s) 55
MroNI GCCGGC 3 cut(s) 55, 85, 152
MscI TGGCCA 2 cut(s) 116, 1659
MseI TTAA 4 cut(s) 324, 1029, 1491, 1797
MslI CAYNNNNRTG 3 cut(s) 1770, 2397, 2424
Msp20I TGGCCA 2 cut(s) 116, 1659
MspA1I CMGCKG 2 cut(s) 224, 245
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 8 cut(s) 56, 86, 101, 153, 157, 266, 314, 452
MspR9I CCNGG 4 cut(s) 76, 158, 1474, 2388
MvaI CCWGG 3 cut(s) 76, 1474, 2388
MvnI CGCG 2 cut(s) 144, 1821
MwoI GCNNNNNNNGC 8 cut(s) 251, 260, 269, 1370, 1619, 1639, 1682, 2279
NaeI GCCGGC 3 cut(s) 57, 87, 154
NciI CCSGG 1 cut(s) 158
NdeI CATATG 2 cut(s) 2478, 2677
NgoMIV GCCGGC 3 cut(s) 55, 85, 152
NlaIV GGNNCC 9 cut(s) 264, 600, 618, 636, 753, 771, 1119, 2508, 2626
NmuCI GTSAC 1 cut(s) 436
NsiI ATGCAT 3 cut(s) 1336, 2352, 2370
NspV TTCGAA 2 cut(s) 203, 2029
PagI TCATGA 2 cut(s) 2494, 2611
PauI GCGCGC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 4 cut(s) 56, 1408, 2035, 2146
PdiI GCCGGC 3 cut(s) 57, 87, 154
PfeI GAWTC 5 cut(s) 200, 653, 671, 1303, 1892
PflFI GACNNNGTC 1 cut(s) 2151
PflMI CCANNNNNTGG 1 cut(s) 2069
PfoI TCCNGGA 1 cut(s) 2386
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 9 cut(s) 54, 91, 271, 415, 1641, 2284, 2551, 2597, 2690
PleI GAGTC 3 cut(s) 481, 703, 925
PpsI GAGTC 3 cut(s) 481, 703, 925
Ppu21I YACGTR 2 cut(s) 1703, 2583
PpuMI RGGWCCY 1 cut(s) 752
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 3 cut(s) 74, 1472, 2386
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 3 cut(s) 74, 1472, 2386
PspN4I GGNNCC 9 cut(s) 264, 600, 618, 636, 753, 771, 1119, 2508, 2626
PspPI GGNCC 5 cut(s) 752, 2384, 2402, 2624, 2642
PspPPI RGGWCCY 1 cut(s) 752
PstI CTGCAG 1 cut(s) 1126
PstNI CAGNNNCTG 3 cut(s) 680, 1501, 2282
PsuI RGATCY 7 cut(s) 598, 616, 634, 769, 1069, 1117, 2066
PsyI GACNNNGTC 1 cut(s) 2151
PteI GCGCGC 1 cut(s) 142
PvuII CAGCTG 2 cut(s) 224, 245
RsaI GTAC 2 cut(s) 250, 297
RsaNI GTAC 2 cut(s) 249, 296
RseI CAYNNNNRTG 3 cut(s) 1770, 2397, 2424
SaqAI TTAA 4 cut(s) 324, 1029, 1491, 1797
SatI GCNGC 9 cut(s) 53, 90, 270, 414, 1640, 2283, 2550, 2596, 2689
Sau96I GGNCC 5 cut(s) 752, 2384, 2402, 2624, 2642
SchI GAGTC 3 cut(s) 482, 704, 926
ScrFI CCNGG 4 cut(s) 76, 158, 1474, 2388
SduI GDGCHC 2 cut(s) 2237, 2275
SfaNI GCATC 7 cut(s) 382, 1099, 1222, 1636, 2314, 2355, 2377
SfcI CTRYAG 2 cut(s) 1122, 1845
SfuI TTCGAA 2 cut(s) 203, 2029
SgrAI CRCCGGYG 3 cut(s) 55, 265, 313
SinI GGWCC 5 cut(s) 752, 2384, 2402, 2624, 2642
SmiI ATTTAAAT 1 cut(s) 1798
SmiMI CAYNNNNRTG 3 cut(s) 1770, 2397, 2424
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SnaBI TACGTA 1 cut(s) 2583
SspMI CTAG 5 cut(s) 389, 1428, 2361, 2381, 2511
StyD4I CCNGG 4 cut(s) 74, 156, 1472, 2386
StyI CCWWGG 2 cut(s) 2315, 2360
SwaI ATTTAAAT 1 cut(s) 1798
TaaI ACNGT 5 cut(s) 130, 520, 2108, 2529, 2696
TaiI ACGT 5 cut(s) 1204, 1448, 1705, 2152, 2585
TaqI TCGA 9 cut(s) 132, 203, 642, 942, 1263, 2029, 2193, 2311, 2560
TauI GCSGC 5 cut(s) 55, 92, 272, 416, 2691
TfiI GAWTC 5 cut(s) 200, 653, 671, 1303, 1892
Tru1I TTAA 4 cut(s) 324, 1029, 1491, 1797
Tru9I TTAA 4 cut(s) 324, 1029, 1491, 1797
TscAI CASTG 6 cut(s) 402, 523, 927, 1669, 2545, 2605
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 4 cut(s) 1639, 2282, 2549, 2595
Tsp45I GTSAC 1 cut(s) 436
TspGWI ACGGA 2 cut(s) 646, 1729
TspRI CASTG 6 cut(s) 402, 523, 927, 1669, 2545, 2605
Tth111I GACNNNGTC 1 cut(s) 2151
Van91I CCANNNNNTGG 1 cut(s) 2069
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 5 cut(s) 752, 2384, 2402, 2624, 2642
XagI CCTNNNNNAGG 1 cut(s) 2334
XapI RAATTY 4 cut(s) 1160, 1592, 1945, 2113
XmaJI CCTAGG 1 cut(s) 2360
XmiI GTMKAC 1 cut(s) 1844
XspI CTAG 5 cut(s) 389, 1428, 2361, 2381, 2511
Zsp2I ATGCAT 3 cut(s) 1336, 2352, 2370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.