MD15G1247300.v1.1

negative regulation of mRNA metabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011510
Physical Location & Seq
Forward (+)
20521194 .. 20525426
4233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1247300.v1.1.491

Sequence Viewer

Length: 2619 bp
ATGAGACCTCGCAAGGCGGACGCTTCCAAACCGCCGACCGCTAAGAAAACGCCGCCGGCGAGGAAAACCGCCGCTAAAGCACAACCGGCGGCTTCCCCGCCGGACTTAGAAGTGGCCATTCTGAAAACCGTCGAAACAAAGCGCGGTTGTGCCGGCCGACTGAAGCAGGCCAAGAAGAACGAAACGACACCGCCTGCTGATTCGAAGGCCCCCAAATCATCAGCTGAGGGGACTGAGGGATCAGCTGGTACTGCTAAAGTGGCACCGGCGGCTAAAGTTGCTGTGGTGAAAAAAGTACCTGCGAAAAGGTTGTCGGCGAAAGTTAAGACACTGGCATCTGCTAAATCTGTTTCACCGCAGACACCGAAATTGGGGGCATCAGTGAAGGCGAAAGCAGATGCATTGAACAAGGGGAAATCCGCAGATGTTAGTGACGTGGAAGTTACAGAAAAGAAAGCGGTTGCTGAGAATGTTGGAGAGTCTGCTAAGAAGGATGGAAGCATTGTGGAATTTGAAGTAAGCACTGTTGAGAATGTTGGAGAGCCTGCAACAGTCAAACAAACAACCGTGGAAGTAAAAGTAAACCCGAATGAAGATGAAGAGGATCCTGAAGAGGAAGAGGATCCTGTTGAGGAGGAGGATCCTATAGAGGAAGAGGATCCCTTTGAGGCAAAAGAATCTGTAGCTGGGGAGGTTTCAGAGTTTTCTGAGAATGAACTAGCTTGTGATGTAAAGGAGGGAGTGGAAGTGACAGAGGAAGAGGATCCCATTGAGGCAGAAAAATCTTTAGTTGGGGAGGTTTCAAAGTCTTCTGAGAATGAACCATCTCGTGATGTAAAAGAGGGAGTGGAAGTGCAAGAAGGTCAGAAGGAGAAGTCGGAGAGAGTAGAAGTGAAAGGAGATCAGAAGAAGGAGTTGGAGGATAATGGAGATGAAGTCGAGACCTGTAGTGAGGCTGCTAACAAGGTAGAAAATTCCAAAGAGGGGTTACAAGGAGAAGATGTTGAGCATGGTAAAGGGGTGTATGATGGTGATGAGCAGATGGAAGAGTATGGTGAGAAATTGGATCTTGGAGAACATGGAGAGGAGGAACTTCCAGAGGATGATGCAGAGGATCCCGCAGAAGAAACTGAGAGATTGGCTGATGAACATAAGGAATTTACAGCCCATGAGCACAAGATGAGGAAAGAACGTGAGATATTTCTTGGTGGGCTTGATCAGGATGCTGTGGAGGAAGATGTGAGAAGGGTCTTTGAGAGGATTGGAGGGATAGTTGAAGTTAGGTTACACAAGAATCCTTCAACTAATAAGCACAAGGGTTATGCATTTGTGGAGTTTGAAAATAAGGAGTATGCCAGGCATGCTTTGTCTGAAATGAAAAACCCTGTTATAAGAGGGAAGCGATGTGGGACAGCACCTAGTGAGGACAATGACATGTTGTTCGTGGGTAATATATGCAATACCTGGACAAAGGAAGCTATTAAACAGAAACTGAAGGATTATGGTGTGGAAGGTGTTGAGAATATAAATCTTGTTCCAGATGTTCAAAGTGATGGATTGAGCCGCGGTTTTGCATTTATTGAATTCTCTTGTCGTGGTGATGCTATGCTTGCATACAAGAGGCTTCAACAGCCCGATGCTATATTTGGCCACTCTGAAAGAACTGCCAAAGTGGCTTTTGCTGAACCTATACGTGAGCCTGACCCAGAGATCATGTCCCAAGTGAAGTCTGTATTTGTCGATGGGCTTCCACCTCATTGGGATGAGGACAGAGTTAGAGAGCAATTTAGATGCTATGGAGAAATCTTACGCGTTGTTGTGGCTCGGAATATGTCTACAGCCAAGCGGAAGGATTTTGGATTTGTTGATTTCTCTACCCATGATTCTGCAGTGGCCTTTGTTGATAGTATAAATAACATAGAACTGATGGATGGGAATTCAAAGGTAAAAGTGAAAGCAAGACTTTCAAATCCTTTGCCTAAAACTCAGGCTGTGAAGGGTGGAATGTGCGGTGGATTTCGAATTGGTCATGGTGGTGGTAGTGGTATCTTTTCAAGATCTGGACGGGGTTTTGGTCGCGTTGGACATCATTTCAACCGTGCAACGTTCCAACCTGATAGACATTTCTATCGTGGTGGACGTGGTCAAACTGGCAGGATGGGTTTCCCGAACGAATATGACTTCGATTACCCATACGAGTTTCATGGACAAGGGGGAAGAAGGGGCTCTTTCAGGGACGGTCGTTATGGATTTGGTGGAGGTGCTCCAGTTGCTGCCCCACCATCAAGACCCTACACAGATCGGACTTGGCATGATGCCCCTGATAGAGGCTATGGTATGCATATTCCTCCTAGGAGGCATCCATATTCTCCAGGACAGTTTGATGGACCATTCATGGGGGGTCATTTTGATGAACCTTATTTTTACAATGATAACGCACATGGAATAAAACGTCCATTTCACATGAGAGACTATGATCATGACTACTCGGAGCCTAGTAGTTGTCGCCCACGGTTGGATTACACTGATCCAGCAGCTTCGTTTCATGGAAACCATTATAGGGGTCTCTTCTCCTCGTCTTGCCCGTATAGTTACTTTTATGTCAGTTTACTATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0006417 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008143 GO:0008150 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016020 GO:0017148 GO:0019219 GO:0019222 GO:0030425 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032270 GO:0032991 GO:0034097 GO:0034248 GO:0034249 GO:0034250 GO:0034341 GO:0036477 GO:0042221 GO:0042995 GO:0043005 GO:0043025 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0043489 GO:0044297 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045087 GO:0045727 GO:0045934 GO:0048027 GO:0048255 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0070717 GO:0070887 GO:0070934 GO:0070937 GO:0071013 GO:0071204 GO:0071310 GO:0071345 GO:0071346 GO:0080090 GO:0090365 GO:0090367 GO:0097159 GO:0097447 GO:0097452 GO:0097458 GO:0120025 GO:0120038 GO:1901363 GO:1902369 GO:1902373 GO:1902494 GO:1903311 GO:1903312 GO:1990635 GO:1990904 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

873

Amino Acids

96.39

Weight (kDa)

5.26

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 400 - 468 1.2e-17 RNA recognition motif
RRM_1 PF00076 480 - 544 1.3e-07 RNA recognition motif
RRM_1 PF00076 578 - 644 2.9e-12 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1391
Acc36I ACCTGC 1 cut(s) 307
AccB1I GGYRCC 1 cut(s) 262
AccI GTMKAC 1 cut(s) 1835
AccII CGCG 4 cut(s) 144, 1566, 1812, 2079
AclI AACGTT 1 cut(s) 2105
AcoI YGGCCR 3 cut(s) 114, 154, 1648
AcsI RAATTY 5 cut(s) 509, 973, 1157, 1583, 1936
AcuI CTGAAG 3 cut(s) 182, 630, 1514
AdeI CACNNNGTG 1 cut(s) 1421
AfaI GTAC 2 cut(s) 250, 297
AfiI CCNNNNNNNGG 6 cut(s) 371, 984, 2327, 2396, 2515, 2560
AflIII ACRYGT 2 cut(s) 1434, 1810
AjiI CACGTC 2 cut(s) 436, 2141
AjnI CCWGG 3 cut(s) 1355, 1463, 2371
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AluBI AGCT 6 cut(s) 224, 245, 686, 722, 1478, 2537
AluI AGCT 6 cut(s) 224, 245, 686, 722, 1478, 2537
Alw21I GWGCWC 2 cut(s) 1176, 2266
Alw26I GTCTC 3 cut(s) 935, 2463, 2570
AlwNI CAGNNNCTG 2 cut(s) 1492, 2273
AoxI GGCC 6 cut(s) 114, 154, 168, 207, 1648, 1893
ApeKI GCWGC 3 cut(s) 956, 2273, 2534
ApoI RAATTY 5 cut(s) 509, 973, 1157, 1583, 1936
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 1424, 1456
AspA2I CCTAGG 1 cut(s) 2351
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 2 cut(s) 208, 2387
AsuHPI GGTGA 5 cut(s) 298, 345, 1043, 1067, 1610
AsuII TTCGAA 2 cut(s) 203, 2020
AvaII GGWCC 1 cut(s) 2387
AvrII CCTAGG 1 cut(s) 2351
BalI TGGCCA 2 cut(s) 116, 1650
BamHI GGATCC 6 cut(s) 604, 622, 640, 658, 763, 1114
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 2228
BarI GAAGNNNNNNTAC 2 cut(s) 1269, 1301
BauI CACGAG 1 cut(s) 828
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 2 cut(s) 1176, 2266
BbvCI CCTCAGC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 943, 2260, 2546
BciT130I CCWGG 3 cut(s) 1357, 1465, 2373
BclI TGATCA 2 cut(s) 1216, 2476
BcoDI GTCTC 3 cut(s) 935, 2463, 2570
BfaI CTAG 4 cut(s) 719, 1419, 2352, 2496
BfmI CTRYAG 5 cut(s) 645, 681, 946, 1836, 1887
BfuAI ACCTGC 1 cut(s) 307
BglI GCCNNNNNGGC 1 cut(s) 1673
BglII AGATCT 1 cut(s) 2057
BisI GCNGC 8 cut(s) 53, 72, 90, 270, 957, 1564, 2274, 2535
BlnI CCTAGG 1 cut(s) 2351
BlsI GCNGC 8 cut(s) 54, 73, 91, 271, 958, 1565, 2275, 2536
Bme1390I CCNGG 3 cut(s) 1357, 1465, 2373
Bme18I GGWCC 1 cut(s) 2387
BmgBI CACGTC 2 cut(s) 436, 2141
BmgT120I GGNCC 2 cut(s) 208, 2387
BmiI GGNNCC 9 cut(s) 210, 264, 606, 624, 642, 660, 765, 1116, 2493
BmrFI CCNGG 3 cut(s) 1357, 1465, 2373
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 2 cut(s) 2250, 2355
Bpu10I CCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 2 cut(s) 203, 2020
BsaAI YACGTR 1 cut(s) 1694
BsaI GGTCTC 2 cut(s) 935, 2570
BsaJI CCNNGG 4 cut(s) 567, 1564, 2351, 2510
Bsc4I CCNNNNNNNGG 6 cut(s) 371, 984, 2327, 2396, 2515, 2560
Bse118I RCCGGY 4 cut(s) 55, 85, 152, 265
Bse1I ACTGG 3 cut(s) 336, 2155, 2267
BseBI CCWGG 3 cut(s) 1357, 1465, 2373
BseDI CCNNGG 4 cut(s) 567, 1564, 2351, 2510
BseGI GGATG 7 cut(s) 499, 1108, 1228, 1768, 1936, 2163, 2359
BseLI CCNNNNNNNGG 6 cut(s) 371, 984, 2327, 2396, 2515, 2560
BseMII CTCAG 7 cut(s) 216, 225, 456, 699, 804, 1122, 2000
BseNI ACTGG 3 cut(s) 336, 2155, 2267
BseRI GAGGAG 4 cut(s) 647, 650, 1100, 2563
BseX3I CGGCCG 1 cut(s) 154
BseXI GCAGC 3 cut(s) 943, 2260, 2546
BseYI CCCAGC 1 cut(s) 686
Bsh1236I CGCG 4 cut(s) 144, 1566, 1812, 2079
Bsh1285I CGRYCG 3 cut(s) 39, 157, 2242
BshFI GGCC 6 cut(s) 116, 156, 170, 209, 1650, 1895
BshNI GGYRCC 1 cut(s) 262
BsiEI CGRYCG 3 cut(s) 39, 157, 2242
BsiHKAI GWGCWC 2 cut(s) 1176, 2266
BsiSI CCGG 5 cut(s) 56, 86, 101, 153, 266
BslFI GGGAC 4 cut(s) 244, 1423, 1702, 2249
BslI CCNNNNNNNGG 6 cut(s) 371, 984, 2327, 2396, 2515, 2560
BsmAI GTCTC 3 cut(s) 935, 2463, 2570
BsmFI GGGAC 4 cut(s) 244, 1423, 1702, 2249
BsnI GGCC 6 cut(s) 116, 156, 170, 209, 1650, 1895
Bso31I GGTCTC 2 cut(s) 935, 2570
Bsp119I TTCGAA 2 cut(s) 203, 2020
Bsp1286I GDGCHC 3 cut(s) 1176, 2228, 2266
BspANI GGCC 6 cut(s) 116, 156, 170, 209, 1650, 1895
BspCNI CTCAG 7 cut(s) 217, 226, 457, 700, 805, 1123, 1999
BspFNI CGCG 4 cut(s) 144, 1566, 1812, 2079
BspHI TCATGA 1 cut(s) 2479
BspLI GGNNCC 9 cut(s) 210, 264, 606, 624, 642, 660, 765, 1116, 2493
BspMAI CTGCAG 1 cut(s) 1891
BspMI ACCTGC 1 cut(s) 307
BspT104I TTCGAA 2 cut(s) 203, 2020
BspT107I GGYRCC 1 cut(s) 262
BspTNI GGTCTC 2 cut(s) 935, 2570
BsrFI RCCGGY 4 cut(s) 55, 85, 152, 265
BsrI ACTGG 3 cut(s) 336, 2155, 2267
BssAI RCCGGY 4 cut(s) 55, 85, 152, 265
BssECI CCNNGG 4 cut(s) 567, 1564, 2351, 2510
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 1 cut(s) 2351
Bst2BI CACGAG 1 cut(s) 828
Bst2UI CCWGG 3 cut(s) 1357, 1465, 2373
Bst4CI ACNGT 8 cut(s) 130, 526, 553, 568, 2099, 2240, 2379, 2514
Bst6I CTCTTC 7 cut(s) 594, 606, 612, 648, 753, 1041, 2573
BstBAI YACGTR 1 cut(s) 1694
BstBI TTCGAA 2 cut(s) 203, 2020
BstC8I GCNNGC 7 cut(s) 57, 154, 168, 195, 546, 1362, 1611
BstDSI CCRYGG 3 cut(s) 567, 1564, 2510
BstENI CCTNNNNNAGG 1 cut(s) 2325
BstF5I GGATG 7 cut(s) 499, 1108, 1228, 1768, 1936, 2163, 2359
BstFNI CGCG 4 cut(s) 144, 1566, 1812, 2079
BstHHI GCGC 1 cut(s) 144
BstMAI GTCTC 3 cut(s) 935, 2463, 2570
BstMCI CGRYCG 3 cut(s) 39, 157, 2242
BstNI CCWGG 3 cut(s) 1357, 1465, 2373
BstNSI RCATGY 2 cut(s) 1364, 1438
BstSCI CCNGG 3 cut(s) 1355, 1463, 2371
BstSFI CTRYAG 5 cut(s) 645, 681, 946, 1836, 1887
BstUI CGCG 4 cut(s) 144, 1566, 1812, 2079
BstV1I GCAGC 3 cut(s) 943, 2260, 2546
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 8 cut(s) 604, 622, 640, 658, 763, 1066, 1114, 2057
BstXI CCANNNNNNTGG 1 cut(s) 1758
BstYI RGATCY 8 cut(s) 604, 622, 640, 658, 763, 1066, 1114, 2057
BstZI CGGCCG 1 cut(s) 154
BsuRI GGCC 6 cut(s) 116, 156, 170, 209, 1650, 1895
BtgI CCRYGG 3 cut(s) 567, 1564, 2510
BtgZI GCGATG 1 cut(s) 1417
BtrI CACGTC 2 cut(s) 436, 2141
BtsCI GGATG 7 cut(s) 499, 1108, 1228, 1768, 1936, 2163, 2359
BtsI GCAGTG 1 cut(s) 1896
BtsIMutI CAGTG 5 cut(s) 329, 387, 522, 1896, 2523
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 7 cut(s) 57, 154, 168, 195, 546, 1362, 1611
CaiI CAGNNNCTG 2 cut(s) 1492, 2273
CciI TCATGA 1 cut(s) 2479
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 4 cut(s) 55, 85, 152, 265
Cfr13I GGNCC 2 cut(s) 208, 2387
Cfr42I CCGCGG 1 cut(s) 1567
CseI GACGC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 249, 296
CviQI GTAC 2 cut(s) 249, 296
DraIII CACNNNGTG 1 cut(s) 1421
EaeI YGGCCR 3 cut(s) 114, 154, 1648
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 7 cut(s) 594, 606, 612, 648, 753, 1041, 2573
EarI CTCTTC 7 cut(s) 594, 606, 612, 648, 753, 1041, 2573
EciI GGCGGA 1 cut(s) 32
EclXI CGGCCG 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 2351
Eco24I GRGCYC 1 cut(s) 2228
Eco31I GGTCTC 2 cut(s) 935, 2570
Eco47I GGWCC 1 cut(s) 2387
Eco52I CGGCCG 1 cut(s) 154
Eco57I CTGAAG 3 cut(s) 182, 630, 1514
EcoNI CCTNNNNNAGG 1 cut(s) 2325
EcoO109I RGGNCCY 1 cut(s) 208
EcoRI GAATTC 2 cut(s) 1583, 1936
EcoRII CCWGG 3 cut(s) 1355, 1463, 2371
EcoT14I CCWWGG 1 cut(s) 2351
EcoT22I ATGCAT 3 cut(s) 403, 1327, 2343
EcoT38I GRGCYC 1 cut(s) 2228
ErhI CCWWGG 1 cut(s) 2351
FalI AAGNNNNNCTT 4 cut(s) 1947, 1979, 2212, 2244
FaqI GGGAC 4 cut(s) 244, 1423, 1702, 2249
FauI CCCGC 2 cut(s) 105, 1126
FbaI TGATCA 2 cut(s) 1216, 2476
FblI GTMKAC 1 cut(s) 1835
Fnu4HI GCNGC 8 cut(s) 53, 72, 90, 270, 957, 1564, 2274, 2535
FokI GGATG 7 cut(s) 506, 1115, 1235, 1775, 1943, 2170, 2346
FriOI GRGCYC 1 cut(s) 2228
Fsp4HI GCNGC 8 cut(s) 53, 72, 90, 270, 957, 1564, 2274, 2535
FspBI CTAG 4 cut(s) 719, 1419, 2352, 2496
GlaI GCGC 1 cut(s) 143
GluI GCNGC 8 cut(s) 53, 72, 90, 270, 957, 1564, 2274, 2535
GsaI CCCAGC 1 cut(s) 690
GsuI CTGGAG 2 cut(s) 2250, 2355
HaeIII GGCC 6 cut(s) 116, 156, 170, 209, 1650, 1895
HapII CCGG 5 cut(s) 56, 86, 101, 153, 266
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 144
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HinfI GANTC 5 cut(s) 200, 479, 677, 1294, 1883
HpaII CCGG 5 cut(s) 56, 86, 101, 153, 266
HphI GGTGA 5 cut(s) 298, 345, 1043, 1067, 1610
Hpy166II GTNNAC 4 cut(s) 583, 1836, 2138, 2609
Hpy8I GTNNAC 4 cut(s) 583, 1836, 2138, 2609
Hpy99I CGWCG 1 cut(s) 134
HpyCH4III ACNGT 8 cut(s) 130, 526, 553, 568, 2099, 2240, 2379, 2514
HpyCH4IV ACGT 6 cut(s) 435, 1192, 1693, 2105, 2140, 2452
HpySE526I ACGT 6 cut(s) 435, 1192, 1693, 2105, 2140, 2452
HspAI GCGC 1 cut(s) 142
KroI GCCGGC 2 cut(s) 55, 152
KroNI GCCGGC 2 cut(s) 57, 154
Ksp22I TGATCA 2 cut(s) 1216, 2476
KspI CCGCGG 1 cut(s) 1567
LmnI GCTCC 2 cut(s) 2269, 2491
Lsp1109I GCAGC 3 cut(s) 943, 2260, 2546
MaeI CTAG 4 cut(s) 719, 1419, 2352, 2496
MaeII ACGT 6 cut(s) 435, 1192, 1693, 2105, 2140, 2452
MaeIII GTNAC 6 cut(s) 431, 442, 748, 987, 1284, 2591
MflI RGATCY 8 cut(s) 604, 622, 640, 658, 763, 1066, 1114, 2057
MhlI GDGCHC 3 cut(s) 1176, 2228, 2266
MlsI TGGCCA 2 cut(s) 116, 1650
MluCI AATT 9 cut(s) 368, 509, 973, 1061, 1157, 1583, 1784, 1936, 2022
MluI ACGCGT 1 cut(s) 1810
MluNI TGGCCA 2 cut(s) 116, 1650
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 7 cut(s) 454, 517, 858, 897, 2062, 2134, 2496
Mox20I TGGCCA 2 cut(s) 116, 1650
Mph1103I ATGCAT 3 cut(s) 403, 1327, 2343
MreI CGCCGGCG 1 cut(s) 55
MroNI GCCGGC 2 cut(s) 55, 152
MscI TGGCCA 2 cut(s) 116, 1650
MseI TTAA 3 cut(s) 324, 1482, 2617
MslI CAYNNNNRTG 3 cut(s) 1761, 2034, 2409
Msp20I TGGCCA 2 cut(s) 116, 1650
MspA1I CMGCKG 3 cut(s) 224, 245, 1566
MspI CCGG 5 cut(s) 56, 86, 101, 153, 266
MspR9I CCNGG 3 cut(s) 1357, 1465, 2373
MvaI CCWGG 3 cut(s) 1357, 1465, 2373
MvnI CGCG 4 cut(s) 144, 1566, 1812, 2079
NaeI GCCGGC 2 cut(s) 57, 154
NgoMIV GCCGGC 2 cut(s) 55, 152
NlaIV GGNNCC 9 cut(s) 210, 264, 606, 624, 642, 660, 765, 1116, 2493
NmuCI GTSAC 2 cut(s) 431, 748
NsiI ATGCAT 3 cut(s) 403, 1327, 2343
NspI RCATGY 2 cut(s) 1364, 1438
NspV TTCGAA 2 cut(s) 203, 2020
PaeI GCATGC 1 cut(s) 1364
PagI TCATGA 1 cut(s) 2479
PciI ACATGT 1 cut(s) 1434
PcsI WCGNNNNNNNCGW 3 cut(s) 56, 2137, 2582
PdiI GCCGGC 2 cut(s) 57, 154
PfeI GAWTC 4 cut(s) 200, 677, 1294, 1883
PflFI GACNNNGTC 1 cut(s) 2142
PfoI TCCNGGA 1 cut(s) 2371
PkrI GCNGC 8 cut(s) 54, 73, 91, 271, 958, 1565, 2275, 2536
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 1694
PscI ACATGT 1 cut(s) 1434
PsiI TTATAA 1 cut(s) 1391
Psp1406I AACGTT 1 cut(s) 2105
Psp6I CCWGG 3 cut(s) 1355, 1463, 2371
PspFI CCCAGC 1 cut(s) 686
PspGI CCWGG 3 cut(s) 1355, 1463, 2371
PspN4I GGNNCC 9 cut(s) 210, 264, 606, 624, 642, 660, 765, 1116, 2493
PspPI GGNCC 2 cut(s) 208, 2387
PstI CTGCAG 1 cut(s) 1891
PstNI CAGNNNCTG 2 cut(s) 1492, 2273
PsuI RGATCY 8 cut(s) 604, 622, 640, 658, 763, 1066, 1114, 2057
PsyI GACNNNGTC 1 cut(s) 2142
PvuII CAGCTG 2 cut(s) 224, 245
RsaI GTAC 2 cut(s) 250, 297
RsaNI GTAC 2 cut(s) 249, 296
RseI CAYNNNNRTG 3 cut(s) 1761, 2034, 2409
SacII CCGCGG 1 cut(s) 1567
SaqAI TTAA 3 cut(s) 324, 1482, 2617
SatI GCNGC 8 cut(s) 53, 72, 90, 270, 957, 1564, 2274, 2535
Sau96I GGNCC 2 cut(s) 208, 2387
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 3 cut(s) 1357, 1465, 2373
SduI GDGCHC 3 cut(s) 1176, 2228, 2266
SfcI CTRYAG 5 cut(s) 645, 681, 946, 1836, 1887
Sfr303I CCGCGG 1 cut(s) 1567
SfuI TTCGAA 2 cut(s) 203, 2020
SgrAI CRCCGGYG 2 cut(s) 55, 265
SgrBI CCGCGG 1 cut(s) 1567
SinI GGWCC 1 cut(s) 2387
SmiMI CAYNNNNRTG 3 cut(s) 1761, 2034, 2409
SphI GCATGC 1 cut(s) 1364
Sse9I AATT 9 cut(s) 368, 509, 973, 1061, 1157, 1583, 1784, 1936, 2022
SspMI CTAG 4 cut(s) 719, 1419, 2352, 2496
StyD4I CCNGG 3 cut(s) 1355, 1463, 2371
StyI CCWWGG 1 cut(s) 2351
TaaI ACNGT 8 cut(s) 130, 526, 553, 568, 2099, 2240, 2379, 2514
TaiI ACGT 6 cut(s) 438, 1195, 1696, 2108, 2143, 2455
TaqI TCGA 6 cut(s) 132, 203, 939, 1740, 2020, 2184
TasI AATT 9 cut(s) 368, 509, 973, 1061, 1157, 1583, 1784, 1936, 2022
TauI GCSGC 5 cut(s) 55, 74, 92, 272, 1566
TfiI GAWTC 4 cut(s) 200, 677, 1294, 1883
Tru1I TTAA 3 cut(s) 324, 1482, 2617
Tru9I TTAA 3 cut(s) 324, 1482, 2617
TscAI CASTG 5 cut(s) 336, 387, 529, 1896, 2530
TseFI GTSAC 2 cut(s) 431, 748
TseI GCWGC 3 cut(s) 956, 2273, 2534
Tsp45I GTSAC 2 cut(s) 431, 748
TspRI CASTG 5 cut(s) 336, 387, 529, 1896, 2530
Tth111I GACNNNGTC 1 cut(s) 2142
VpaK11BI GGWCC 1 cut(s) 2387
XagI CCTNNNNNAGG 1 cut(s) 2325
XapI RAATTY 5 cut(s) 509, 973, 1157, 1583, 1936
XceI RCATGY 2 cut(s) 1364, 1438
XmaJI CCTAGG 1 cut(s) 2351
XmiI GTMKAC 1 cut(s) 1835
XspI CTAG 4 cut(s) 719, 1419, 2352, 2496
Zsp2I ATGCAT 3 cut(s) 403, 1327, 2343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.