Rw2G010160

negative regulation of mRNA metabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011510
Physical Location & Seq
Forward (+)
10278916 .. 10283197
4282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010160.1

Sequence Viewer

Length: 2973 bp
ATGAAAACTCGCAATGCCGGCGTTCCTAAACCGTCGACGACCGCAAAGAAAACGCCGCCGGCGAGAAAATCCGCCTCCAAAGCACAGCCGTCTCCGTCAGTAGAGACTCCTAAAACCGTCGAGACAAAGCGGACTTCTGCCAGAGCAGCCGAGCAGGTCAAGAAGACTGAAACCGCTCCCGTTACTGCTTCAAAGCGCCGTCAATCCTCAGCCGAGGACTCGGCTGGTGGTGCTAAGGGGACGCTAGGCACTAAAGTTGGTGTGGGAACTTCACCAATAGGGAGAAGGACTTCCGGGAGAGCAAAGACACCGGCATCGGTGTATGTTTCAGCACAGGCACAGTCTGCCAAGGCAAAAGGAAATGCATCGAATAAGAAGAAAAAGGTAGATATCAAGGATGTGGAGTTCACAGAGGAGGAGGTCATGGCTGAATTTGATGGAAAACCTGCAGGGTTGGAAGAAACAGCTGTGGGGGCAGAAGTACCAGCCGTTGAGAATGTTGGAGATCCTATTGAGATGGAAGAATCTGTAGTTAAGGAGGTTGCAAAGTCTTCAGAGAATGTGCCGCGCTGTGATGAAGGGGATCCTGTTGAAGTAGAAGAATCTGTGATTGAGGAGGTTGCAAAGTCTTCAGAGTGTGTGCCGCTGTGTGATGAGGAGGATCCTGTTGAGAAAGAAGAATCTGTAGTTGGGGAGGCTGCAAGGTCTTCAGAGAATGAACCACCCTGTGACAAAGAAAGTCCTGTTGCGATAGAAGAATCTGTAATCGAGGAGGTTGCAAAGTCTTCAGAGAATGAACTGCCCTGTGACGAAGATCCTGTTGCGATAGAAGAATCTGTAATAGAGGAGATTGCAAGGTCTTCAGGAAATGAGCCGGCCTGTGATATAAGACAATCTGTAGTTGAGGAGGTCGCAAGGAATTCAGAGAATGAGCCGCCCTGCGCTGAAGAGGATCCTACTGAGATAGAAGAGTCTGTAATTGAGGTTGTTACAAAGTCTTCAGAAAATAGATTACCCTGCTATGTAAGAGAAAGAATGGAAGTGAAAGCAGAACAGAAGGAAGAGTCTAAGGATAATTATAGTAATGAGGAGACCTCTTTTGAGCCTGCTAACAAGGTACAAAATGACAAAGCAAGGTTACAAGGAGAAGATACTGATCTTCCAAAAGAGATGTATGATGTTGATGAGGAAATGGAATATGGTGAGAAAATTGATCTTGGAGAACATGGAGAGGAAGAACTTCCGGGAGATGATGCAGAGGATCATGAAGAAGAAACTGAGACATTGGAGGATGAACGCAAGCAGTTGACAACCATCGCAAAGGAGCGCAAGATAAAGAAAGAGCGGGAGGTATTTCTTGATGGTCTTGATCCGGATGTTGAGGAGGAAGACGTGAGAAGGGTCTTTGAGAGGATTGGAGGGATAGTTGAGGTTAGGTTACAGAAGAATCCTTCAACTAATAAGAATAAGGGTTATGCATATGTGGAGTTCAAAGATAAGGAGCATGTGAGGCGTGCTTTGTCTGAAATGAAAAACCCTGCTATTCGAGGGAAACGATGTGGGACAGCACCCAGTGAGGACAATACCACGTTGTTCATAGGTAACATCTGCAATACGTGGACAAAGGAAGCGATTAAACAAAAACTAAAGGATTATGAGGTTGAAGGTGTTGAGAACATAAACCTTGTCCCGGATATTCAACGTGAAGGGTTGAGCCGAGGTTTTGCATTTGTTGAGTTCTCTTGTCATGGTGATGCTATGCTTGCATACAAGAGGCTTCAGCAGTCTGATGTTATATTTGGCCACCCTGAAAGGACTGCCAAAGTGGCCTTTGCTGAACCTATATGTGAGCCTGACCCTGAGGTCATGGCCCAAGTGAAGTCAATATTTGTGGATGGGCTTCCACCTCATTGGGATGAGAAACAGGTTAGAGAGCAATTTAAATGCTATGGAGAAATATTACGTGTTGTTCTGGCCCGAAATATGTCTACAGCCAAGCGGAAAGATTTTGGATTTGTTGATTTCTCTAGCCACGAGTCTGCTGTGACATGTGTTAATGTTATAAACAATACACCACTGAGTGATGGGCATTTAAAGATAAAAGTGAAAGCAAGACTTTCAAATCCTCTGCCTAAAACTCAGGCTGTGAAGGGCGGAATACGTGGTGGATTTCGAATTGGCCGTGGTGGCAGCGGTGTTTCAAGATTTGGTAGGGATTTTGGTCGCAGTGGACACACTTCCAATCATGCAAATTTCCAACATGACAGACATTTTTATGACGGTGGACGTGGTCAAACTGCGAGGATGGGTTTCAATCAGCATAACTATGATTATCCATATGCTGAGTTTCGTGGACGAGGGGGCAGAAGGGGCTTTTTTAGAGGTGGTCAATTTTCATCTGGTGGAGGTTCTGGAGTTGCTTCCCCATCACCAAGACCTTATGTTGATAGATCTTCGCATGGTGCCCCTGATAGAGGGCGAGGTATGCCTGCTCCTTTTAGGAGGCATCCTTATTCTCCAGGACACTTTGATGGGCCACACATGAGGGGTCACTTTGATGGGCCACACATGAGGGGTCACTTTGATGGGCCACACACGGGGGGTCACTTTGATGGGCCACACATGGGTGGTCACTTTGATGGGCCACACATTGGTGGTCACTTTGATGGGCCACACATAGGTGGTCACTTTGATGAACCTTCTTTTTATGATGAACGTGATAGAACACAAGGCATCAAGCGCCCATTTCATATGAGAGACTATGATCCTGATTACTTGGAGCCTAATAGACATCGGCCACGATTGAATTATAATGATCCAACAGCTTCATTTCGCGGAAACCATTACAGTGATACTTATGGAGCTGGTAGCAGTCTCTACCCTCATGAATATTATGGTCCTGAATCTCATTATGGTCCACATTCATCTTATTATGGGCGCAATCAATCATATGGTGGGGGTCGCTACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005737 GO:0006417 GO:0006950 GO:0006952 GO:0006955 GO:0007623 GO:0008143 GO:0008150 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016020 GO:0017148 GO:0019219 GO:0019222 GO:0030425 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032270 GO:0032991 GO:0034097 GO:0034248 GO:0034249 GO:0034250 GO:0034341 GO:0036477 GO:0042221 GO:0042995 GO:0043005 GO:0043025 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0043489 GO:0044297 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0045087 GO:0045727 GO:0045934 GO:0048027 GO:0048255 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0070717 GO:0070887 GO:0070934 GO:0070937 GO:0071013 GO:0071204 GO:0071310 GO:0071345 GO:0071346 GO:0080090 GO:0090365 GO:0090367 GO:0097159 GO:0097447 GO:0097452 GO:0097458 GO:0120025 GO:0120038 GO:1901363 GO:1902369 GO:1902373 GO:1902494 GO:1903311 GO:1903312 GO:1990635 GO:1990904 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

990

Amino Acids

109.8

Weight (kDa)

5.4

Isoelectric Point (pI)

58.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 451 - 519 1.5e-15 RNA recognition motif
RRM_1 PF00076 531 - 595 1.3e-07 RNA recognition motif
RRM_1 PF00076 629 - 693 1.1e-11 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2063, 2811
AasI GACNNNNNNGTC 1 cut(s) 1862
Acc36I ACCTGC 2 cut(s) 145, 454
AccB1I GGYRCC 1 cut(s) 2462
AccB7I CCANNNNNTGG 1 cut(s) 2651
AccBSI CCGCTC 2 cut(s) 176, 1345
AccI GTMKAC 2 cut(s) 35, 1988
AccII CGCG 2 cut(s) 568, 2835
AccIII TCCGGA 1 cut(s) 1372
AcoI YGGCCR 3 cut(s) 1801, 2179, 2795
AcsI RAATTY 3 cut(s) 431, 919, 2251
AcuI CTGAAG 8 cut(s) 537, 615, 693, 771, 846, 966, 984, 1763
AdeI CACNNNGTG 3 cut(s) 728, 1574, 2081
AfaI GTAC 2 cut(s) 483, 1119
AfiI CCNNNNNNNGG 6 cut(s) 1690, 2474, 2597, 2624, 2651, 2678
AflIII ACRYGT 2 cut(s) 1963, 2048
AjiI CACGTC 2 cut(s) 1393, 2288
AjnI CCWGG 1 cut(s) 2518
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
AleI CACNNNNGTG 3 cut(s) 2625, 2652, 2679
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
AluBI AGCT 3 cut(s) 467, 2825, 2864
AluI AGCT 3 cut(s) 467, 2825, 2864
Alw26I GTCTC 7 cut(s) 96, 98, 116, 1085, 1274, 2751, 2879
Aor13HI TCCGGA 1 cut(s) 1372
ApeKI GCWGC 3 cut(s) 146, 698, 2190
ApoI RAATTY 3 cut(s) 431, 919, 2251
Asp700I GAANNNNTTC 2 cut(s) 289, 1239
AspLEI GCGC 6 cut(s) 198, 570, 944, 1329, 2742, 2940
AsuC2I CCSGG 3 cut(s) 295, 1245, 1691
AsuHPI GGTGA 4 cut(s) 264, 1214, 1763, 2421
AsuII TTCGAA 1 cut(s) 2173
AvaII GGWCC 2 cut(s) 2897, 2915
AxyI CCTNAGG 1 cut(s) 1860
BaeGI GKGCMC 1 cut(s) 2467
BalI TGGCCA 1 cut(s) 1803
BamHI GGATCC 3 cut(s) 583, 661, 952
BanI GGYRCC 1 cut(s) 2462
BauI CACGAG 1 cut(s) 2033
BbsI GAAGAC 8 cut(s) 170, 543, 621, 699, 777, 852, 990, 1395
BbvCI CCTCAGC 1 cut(s) 208
BbvI GCAGC 3 cut(s) 158, 685, 2202
BceAI ACGGC 4 cut(s) 73, 183, 473, 2166
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciT130I CCWGG 1 cut(s) 2520
BcnI CCSGG 3 cut(s) 295, 1245, 1691
BcoDI GTCTC 7 cut(s) 96, 98, 116, 1085, 1274, 2751, 2879
BfaI CTAG 2 cut(s) 245, 2028
BfmI CTRYAG 5 cut(s) 447, 528, 684, 897, 1989
BfoI RGCGCY 2 cut(s) 199, 2743
BfuAI ACCTGC 2 cut(s) 145, 454
BglI GCCNNNNNGGC 2 cut(s) 1826, 2187
BglII AGATCT 1 cut(s) 2450
BisI GCNGC 7 cut(s) 56, 147, 566, 644, 699, 935, 2191
BlsI GCNGC 7 cut(s) 57, 148, 567, 645, 700, 936, 2192
Bme1390I CCNGG 4 cut(s) 295, 1245, 1691, 2520
Bme18I GGWCC 2 cut(s) 2897, 2915
BmgBI CACGTC 2 cut(s) 1393, 2288
BmiI GGNNCC 5 cut(s) 585, 663, 954, 2464, 2781
BmrFI CCNGG 4 cut(s) 295, 1245, 1691, 2520
BmrI ACTGGG 1 cut(s) 1566
BmsI GCATC 6 cut(s) 323, 374, 1243, 1744, 2515, 2742
BmuI ACTGGG 1 cut(s) 1566
BpiI GAAGAC 8 cut(s) 170, 543, 621, 699, 777, 852, 990, 1395
BplI GAGNNNNNCTC 2 cut(s) 1079, 1111
BpmI CTGGAG 2 cut(s) 2433, 2502
Bpu10I CCTNAGC 2 cut(s) 208, 234
Bpu14I TTCGAA 1 cut(s) 2173
BpuMI CCSGG 3 cut(s) 295, 1245, 1691
BsaAI YACGTR 3 cut(s) 1617, 1964, 2162
BsaBI GATNNNNATC 1 cut(s) 1155
BsaI GGTCTC 1 cut(s) 1085
BsaJI CCNNGG 4 cut(s) 213, 348, 1717, 2182
BsaWI WCCGGW 1 cut(s) 1372
BsaXI ACNNNNNCTCC 2 cut(s) 1410, 1440
Bsc4I CCNNNNNNNGG 6 cut(s) 1690, 2474, 2597, 2624, 2651, 2678
Bse118I RCCGGY 4 cut(s) 17, 58, 310, 874
Bse1I ACTGG 1 cut(s) 1572
Bse21I CCTNAGG 1 cut(s) 1860
Bse3DI GCAATG 1 cut(s) 19
Bse8I GATNNNNATC 1 cut(s) 1155
BseAI TCCGGA 1 cut(s) 1372
BseBI CCWGG 1 cut(s) 2520
BseDI CCNNGG 4 cut(s) 213, 348, 1717, 2182
BseGI GGATG 7 cut(s) 403, 1297, 1381, 1900, 1921, 2310, 2506
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 6 cut(s) 1690, 2474, 2597, 2624, 2651, 2678
BseMI GCAATG 1 cut(s) 19
BseMII CTCAG 7 cut(s) 222, 951, 1269, 1851, 2069, 2153, 2334
BseNI ACTGG 1 cut(s) 1572
BseRI GAGGAG 9 cut(s) 428, 431, 629, 671, 785, 860, 920, 1103, 1397
BseSI GKGCMC 1 cut(s) 2467
BseXI GCAGC 3 cut(s) 158, 685, 2202
Bsh1236I CGCG 2 cut(s) 568, 2835
Bsh1285I CGRYCG 1 cut(s) 42
BshNI GGYRCC 1 cut(s) 2462
BsiEI CGRYCG 1 cut(s) 42
BsiSI CCGG 8 cut(s) 18, 59, 294, 311, 875, 1244, 1373, 1691
BslFI GGGAC 3 cut(s) 253, 1576, 1673
BslI CCNNNNNNNGG 6 cut(s) 1690, 2474, 2597, 2624, 2651, 2678
BsmAI GTCTC 7 cut(s) 96, 98, 116, 1085, 1274, 2751, 2879
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 3 cut(s) 253, 1576, 1673
Bso31I GGTCTC 1 cut(s) 1085
Bsp119I TTCGAA 1 cut(s) 2173
Bsp1286I GDGCHC 1 cut(s) 2467
Bsp13I TCCGGA 1 cut(s) 1372
BspCNI CTCAG 7 cut(s) 221, 952, 1270, 1852, 2070, 2152, 2335
BspEI TCCGGA 1 cut(s) 1372
BspFNI CGCG 2 cut(s) 568, 2835
BspHI TCATGA 2 cut(s) 1264, 2884
BspLI GGNNCC 5 cut(s) 585, 663, 954, 2464, 2781
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 2 cut(s) 145, 454
BspT104I TTCGAA 1 cut(s) 2173
BspT107I GGYRCC 1 cut(s) 2462
BspTNI GGTCTC 1 cut(s) 1085
BsrBI CCGCTC 2 cut(s) 176, 1345
BsrDI GCAATG 1 cut(s) 19
BsrFI RCCGGY 4 cut(s) 17, 58, 310, 874
BsrI ACTGG 1 cut(s) 1572
BssAI RCCGGY 4 cut(s) 17, 58, 310, 874
BssECI CCNNGG 4 cut(s) 213, 348, 1717, 2182
BssSI CACGAG 1 cut(s) 2033
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 2033
Bst2UI CCWGG 1 cut(s) 2520
Bst4CI ACNGT 5 cut(s) 33, 118, 342, 2282, 2849
Bst6I CTCTTC 3 cut(s) 942, 963, 1056
BstAPI GCANNNNNTGC 1 cut(s) 344
BstBAI YACGTR 3 cut(s) 1617, 1964, 2162
BstBI TTCGAA 1 cut(s) 2173
BstC8I GCNNGC 8 cut(s) 19, 60, 876, 1107, 1301, 1515, 1764, 2490
BstDEI CTNAG 9 cut(s) 208, 234, 960, 1068, 1278, 1860, 2078, 2139, 2343
BstDSI CCRYGG 1 cut(s) 2182
BstENI CCTNNNNNAGG 1 cut(s) 2472
BstF5I GGATG 7 cut(s) 403, 1297, 1381, 1900, 1921, 2310, 2506
BstFNI CGCG 2 cut(s) 568, 2835
BstH2I RGCGCY 2 cut(s) 199, 2743
BstHHI GCGC 6 cut(s) 198, 570, 944, 1329, 2742, 2940
BstMAI GTCTC 7 cut(s) 96, 98, 116, 1085, 1274, 2751, 2879
BstMCI CGRYCG 1 cut(s) 42
BstNI CCWGG 1 cut(s) 2520
BstNSI RCATGY 2 cut(s) 1508, 2052
BstSCI CCNGG 4 cut(s) 293, 1243, 1689, 2518
BstSFI CTRYAG 5 cut(s) 447, 528, 684, 897, 1989
BstSLI GKGCMC 1 cut(s) 2467
BstUI CGCG 2 cut(s) 568, 2835
BstV1I GCAGC 3 cut(s) 158, 685, 2202
BstV2I GAAGAC 8 cut(s) 170, 543, 621, 699, 777, 852, 990, 1395
BstX2I RGATCY 6 cut(s) 505, 583, 661, 814, 952, 2450
BstXI CCANNNNNNTGG 1 cut(s) 1911
BstYI RGATCY 6 cut(s) 505, 583, 661, 814, 952, 2450
Bsu36I CCTNAGG 1 cut(s) 1860
BtgI CCRYGG 1 cut(s) 2182
BtgZI GCGATG 1 cut(s) 1300
BtrI CACGTC 2 cut(s) 1393, 2288
BtsCI GGATG 7 cut(s) 403, 1297, 1381, 1900, 1921, 2310, 2506
BtsI GCAGTG 1 cut(s) 2233
BtsIMutI CAGTG 4 cut(s) 1579, 2075, 2233, 2854
BveI ACCTGC 2 cut(s) 145, 454
Cac8I GCNNGC 8 cut(s) 19, 60, 876, 1107, 1301, 1515, 1764, 2490
CciI TCATGA 2 cut(s) 1264, 2884
CfoI GCGC 6 cut(s) 198, 570, 944, 1329, 2742, 2940
Cfr10I RCCGGY 4 cut(s) 17, 58, 310, 874
CseI GACGC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 482, 1118
CspCI CAANNNNNGTGG 2 cut(s) 1872, 1907
CviQI GTAC 2 cut(s) 482, 1118
DdeI CTNAG 9 cut(s) 208, 234, 960, 1068, 1278, 1860, 2078, 2139, 2343
DraI TTTAAA 2 cut(s) 1942, 2094
DraIII CACNNNGTG 3 cut(s) 728, 1574, 2081
DrdI GACNNNNNNGTC 1 cut(s) 1862
DseDI GACNNNNNNGTC 1 cut(s) 1862
EaeI YGGCCR 3 cut(s) 1801, 2179, 2795
Eam1104I CTCTTC 3 cut(s) 942, 963, 1056
EarI CTCTTC 3 cut(s) 942, 963, 1056
EciI GGCGGA 2 cut(s) 61, 2169
Eco130I CCWWGG 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 1085
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 2897, 2915
Eco57I CTGAAG 8 cut(s) 537, 615, 693, 771, 846, 966, 984, 1763
Eco81I CCTNAGG 1 cut(s) 1860
EcoNI CCTNNNNNAGG 1 cut(s) 2472
EcoRI GAATTC 1 cut(s) 919
EcoRII CCWGG 1 cut(s) 2518
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 2 cut(s) 367, 1480
ErhI CCWWGG 1 cut(s) 348
Esp3I CGTCTC 1 cut(s) 96
FalI AAGNNNNNCTT 2 cut(s) 2100, 2132
FaqI GGGAC 3 cut(s) 253, 1576, 1673
FauI CCCGC 1 cut(s) 1338
FauNDI CATATG 4 cut(s) 1480, 2338, 2751, 2950
FblI GTMKAC 2 cut(s) 35, 1988
Fnu4HI GCNGC 7 cut(s) 56, 147, 566, 644, 699, 935, 2191
FokI GGATG 7 cut(s) 410, 1304, 1388, 1907, 1928, 2317, 2493
Fsp4HI GCNGC 7 cut(s) 56, 147, 566, 644, 699, 935, 2191
FspBI CTAG 2 cut(s) 245, 2028
GlaI GCGC 6 cut(s) 197, 569, 943, 1328, 2741, 2939
GluI GCNGC 7 cut(s) 56, 147, 566, 644, 699, 935, 2191
GsuI CTGGAG 2 cut(s) 2433, 2502
HaeII RGCGCY 2 cut(s) 199, 2743
HapII CCGG 8 cut(s) 18, 59, 294, 311, 875, 1244, 1373, 1691
HgaI GACGC 1 cut(s) 250
HhaI GCGC 6 cut(s) 198, 570, 944, 1329, 2742, 2940
Hin6I GCGC 6 cut(s) 196, 568, 942, 1327, 2740, 2938
HinP1I GCGC 6 cut(s) 196, 568, 942, 1327, 2740, 2938
HincII GTYRAC 2 cut(s) 36, 1308
HindII GTYRAC 2 cut(s) 36, 1308
HpaII CCGG 8 cut(s) 18, 59, 294, 311, 875, 1244, 1373, 1691
HphI GGTGA 4 cut(s) 264, 1214, 1763, 2421
Hpy166II GTNNAC 9 cut(s) 36, 408, 1308, 1620, 1989, 2231, 2285, 2354, 2918
Hpy188I TCNGA 8 cut(s) 556, 634, 712, 790, 925, 1003, 1525, 1789
Hpy8I GTNNAC 9 cut(s) 36, 408, 1308, 1620, 1989, 2231, 2285, 2354, 2918
Hpy99I CGWCG 3 cut(s) 37, 40, 122
HpyCH4III ACNGT 5 cut(s) 33, 118, 342, 2282, 2849
HpyCH4IV ACGT 8 cut(s) 1392, 1589, 1616, 1702, 1963, 2161, 2287, 2716
HpyF3I CTNAG 9 cut(s) 208, 234, 960, 1068, 1278, 1860, 2078, 2139, 2343
HpySE526I ACGT 8 cut(s) 1392, 1589, 1616, 1702, 1963, 2161, 2287, 2716
HspAI GCGC 6 cut(s) 196, 568, 942, 1327, 2740, 2938
Kpn2I TCCGGA 1 cut(s) 1372
KroI GCCGGC 3 cut(s) 17, 58, 874
KroNI GCCGGC 3 cut(s) 19, 60, 876
LmnI GCTCC 6 cut(s) 181, 1324, 1501, 2497, 2779, 2861
Lsp1109I GCAGC 3 cut(s) 158, 685, 2202
LweI GCATC 6 cut(s) 323, 374, 1243, 1744, 2515, 2742
MaeI CTAG 2 cut(s) 245, 2028
MaeII ACGT 8 cut(s) 1392, 1589, 1616, 1702, 1963, 2161, 2287, 2716
MbiI CCGCTC 2 cut(s) 176, 1345
MflI RGATCY 6 cut(s) 505, 583, 661, 814, 952, 2450
MhlI GDGCHC 1 cut(s) 2467
MlsI TGGCCA 1 cut(s) 1803
MluNI TGGCCA 1 cut(s) 1803
MlyI GAGTC 5 cut(s) 100, 212, 980, 1073, 2045
MmeI TCCRAC 4 cut(s) 435, 481, 2281, 2843
Mox20I TGGCCA 1 cut(s) 1803
Mph1103I ATGCAT 2 cut(s) 367, 1480
MreI CGCCGGCG 1 cut(s) 58
MroI TCCGGA 1 cut(s) 1372
MroNI GCCGGC 3 cut(s) 17, 58, 874
MroXI GAANNNNTTC 2 cut(s) 289, 1239
MscI TGGCCA 1 cut(s) 1803
MseI TTAA 5 cut(s) 534, 1635, 1941, 2055, 2093
Msp20I TGGCCA 1 cut(s) 1803
MspA1I CMGCKG 3 cut(s) 467, 646, 2193
MspI CCGG 8 cut(s) 18, 59, 294, 311, 875, 1244, 1373, 1691
MspR9I CCNGG 4 cut(s) 295, 1245, 1691, 2520
MvaI CCWGG 1 cut(s) 2520
MvnI CGCG 2 cut(s) 568, 2835
NaeI GCCGGC 3 cut(s) 19, 60, 876
NciI CCSGG 3 cut(s) 295, 1245, 1691
NdeI CATATG 4 cut(s) 1480, 2338, 2751, 2950
NgoMIV GCCGGC 3 cut(s) 17, 58, 874
NlaIV GGNNCC 5 cut(s) 585, 663, 954, 2464, 2781
NmeAIII GCCGAG 4 cut(s) 175, 200, 238, 1742
NmuCI GTSAC 9 cut(s) 728, 806, 2044, 2549, 2576, 2603, 2630, 2657, 2684
NsiI ATGCAT 2 cut(s) 367, 1480
NspI RCATGY 2 cut(s) 1508, 2052
NspV TTCGAA 1 cut(s) 2173
OliI CACNNNNGTG 3 cut(s) 2625, 2652, 2679
PagI TCATGA 2 cut(s) 1264, 2884
PciI ACATGT 1 cut(s) 2048
PcsI WCGNNNNNNNCGW 3 cut(s) 59, 1552, 2179
PdiI GCCGGC 3 cut(s) 19, 60, 876
PdmI GAANNNNTTC 2 cut(s) 289, 1239
PfeI GAWTC 7 cut(s) 524, 602, 680, 758, 833, 1447, 2903
PflFI GACNNNGTC 1 cut(s) 2289
PflMI CCANNNNNTGG 1 cut(s) 2651
PfoI TCCNGGA 4 cut(s) 293, 1243, 1689, 2518
PkrI GCNGC 7 cut(s) 57, 148, 567, 645, 700, 936, 2192
PleI GAGTC 5 cut(s) 100, 212, 979, 1072, 2044
PpsI GAGTC 5 cut(s) 100, 212, 979, 1072, 2044
Ppu21I YACGTR 3 cut(s) 1617, 1964, 2162
PscI ACATGT 1 cut(s) 2048
PsiI TTATAA 2 cut(s) 2063, 2811
Psp6I CCWGG 1 cut(s) 2518
PspGI CCWGG 1 cut(s) 2518
PspN4I GGNNCC 5 cut(s) 585, 663, 954, 2464, 2781
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 6 cut(s) 505, 583, 661, 814, 952, 2450
PsyI GACNNNGTC 1 cut(s) 2289
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 2 cut(s) 483, 1119
RsaNI GTAC 2 cut(s) 482, 1118
SalI GTCGAC 1 cut(s) 34
SaqAI TTAA 5 cut(s) 534, 1635, 1941, 2055, 2093
SatI GCNGC 7 cut(s) 56, 147, 566, 644, 699, 935, 2191
SbfI CCTGCAGG 1 cut(s) 451
SchI GAGTC 5 cut(s) 100, 212, 980, 1073, 2045
ScrFI CCNGG 4 cut(s) 295, 1245, 1691, 2520
SdaI CCTGCAGG 1 cut(s) 451
SduI GDGCHC 1 cut(s) 2467
SfaNI GCATC 6 cut(s) 323, 374, 1243, 1744, 2515, 2742
SfcI CTRYAG 5 cut(s) 447, 528, 684, 897, 1989
SfuI TTCGAA 1 cut(s) 2173
SgrAI CRCCGGYG 1 cut(s) 58
SgrDI CGTCGACG 1 cut(s) 34
SinI GGWCC 2 cut(s) 2897, 2915
SmiI ATTTAAAT 1 cut(s) 1942
Sse8387I CCTGCAGG 1 cut(s) 451
SspI AATATT 3 cut(s) 1887, 1959, 2891
SspMI CTAG 2 cut(s) 245, 2028
StyD4I CCNGG 4 cut(s) 293, 1243, 1689, 2518
StyI CCWWGG 1 cut(s) 348
SwaI ATTTAAAT 1 cut(s) 1942
TaaI ACNGT 5 cut(s) 33, 118, 342, 2282, 2849
TaiI ACGT 8 cut(s) 1395, 1592, 1619, 1705, 1966, 2164, 2290, 2719
TaqI TCGA 6 cut(s) 35, 120, 368, 768, 1546, 2173
TauI GCSGC 4 cut(s) 58, 568, 646, 937
TfiI GAWTC 7 cut(s) 524, 602, 680, 758, 833, 1447, 2903
Tru1I TTAA 5 cut(s) 534, 1635, 1941, 2055, 2093
Tru9I TTAA 5 cut(s) 534, 1635, 1941, 2055, 2093
TscAI CASTG 4 cut(s) 1579, 2082, 2233, 2854
TseFI GTSAC 9 cut(s) 728, 806, 2044, 2549, 2576, 2603, 2630, 2657, 2684
TseI GCWGC 3 cut(s) 146, 698, 2190
Tsp45I GTSAC 9 cut(s) 728, 806, 2044, 2549, 2576, 2603, 2630, 2657, 2684
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 4 cut(s) 1579, 2082, 2233, 2854
Tth111I GACNNNGTC 1 cut(s) 2289
Van91I CCANNNNNTGG 1 cut(s) 2651
VpaK11BI GGWCC 2 cut(s) 2897, 2915
XagI CCTNNNNNAGG 1 cut(s) 2472
XapI RAATTY 3 cut(s) 431, 919, 2251
XceI RCATGY 2 cut(s) 1508, 2052
XmiI GTMKAC 2 cut(s) 35, 1988
XmnI GAANNNNTTC 2 cut(s) 289, 1239
XspI CTAG 2 cut(s) 245, 2028
Zsp2I ATGCAT 2 cut(s) 367, 1480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.