MD02G1170800.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012565
Physical Location & Seq
Forward (+)
14759097 .. 14762114
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1170800.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGGCTCGGAAGGTTGACGGGAAGCCCCAAACGCCGCCGTCCAAACCCAGGCTCCGCCCCAAGCCCGCCGCCAACCCTAAACCTTCTTCTCTCCCCACGGCTAAGGTGAAGAAGCCAGGCAAGCCGAACGAGAAGGAAGTGGAGCATTCCATGCCTATGAAGGAGCTTTCCAATACCCAAATTACAAAGATGTTGAGTCATGCAGGGAAGTCCCCAGCCATTAGCAATACACAGTATGAGTTTCCAGTTGATGGATCACCCTCACGAGGTCAAGCAAAGTCATCTGCTGTCATTCGAGCACAGGAAGTTCGATCAAAACTAGAACGTCAATTCCCTAGCTTCGTGAAATCACTGGTCAGATCACACGTGGCCAGTTGTTTTTGGATGGGGCTTCCTTCGCTGTTCTGCAAATCACACTTACCAGATAAAGATATTACAATGACTTTGGAAGATGAAAGTGGTAGACAGTATCAGTTGAAATACATTGCATGCAAGACCGGACTCAGTGCTGGTTGGAGACAGTTTGCTGCTTCTCACAATTTACTCGAGGGAGATGTTCTGGTCTTCCAATTAGTCGAACTTACAAAATTTAAGGTTTACATAATAAGGTCAAATGGTATGGCTGAAGTTGATGGGGCTCTTGGCCTTCTAAATTTGGATAATAATACAAAACAAAAGGATTCAGATAAAGATAATGCAGAAACGAGCACTACGCCTTGTAATGACTCCATCAGGAAACGCGCACATTCTCCTCAGTTAGTAGTTCCAAAGAAGAAAAAGATTGGTTTGCAGCGAGCCGAACAATCTGAAAACGATAGTGAGGACGCTGGTTCAGAAGTTTCAGAAGATCTCAAGTCACCTGCCGCAGCTCTTCAATTTGAGGACATCCGAAGTTATGAGAATTTTCACATTCTAGTAGATGGGTTGTTTCTAGATTCTGAGTTCCCCGAAGATGTTCGAAAGAAGTACTACAAGCTCTGCCGCAGCCAGAAAGCGTTTCTTCATGAAAATGTTATCAAGGGCATGAATCCTAATTTGATTATAGGAGCTATATCTGAAACTGTAAATATTGCCGATTCCATTAAAGCTTGCAAGGTCACTACACATCGAGATGATTTTGTGTCTTGGGACAAGTATTTAACGGCCTTTGAGTTGTTGGGCATGAATGTTGGATTTTTACGTGATCGGATATGTCGGCTTGCAAACCTAGCCGACGACTCAGGAGCTGCATTGGAGGCAAAAATGTACAAAGAAGCTAGAATTGAGCGGTCGCAGGCAGGAATAGGAATAAGAAATATCGAAGTGAAGCTGGAGGAAATGAGGGAGGCGTGCGATGAATTCGGCGCTGCCATTGAGAGCCTGAGATCAAAAGCTGAAATCAACACGCTCAAGTTCCAGGAAGAGGTTACTGCTCCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

474

Amino Acids

52.82

Weight (kDa)

8.68

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 114 - 204 8.2e-18 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 868
Acc36I ACCTGC 1 cut(s) 868
AccB7I CCANNNNNTGG 1 cut(s) 251
AccBSI CCGCTC 1 cut(s) 1267
AccI GTMKAC 1 cut(s) 463
AccII CGCG 1 cut(s) 741
AciI CCGC 7 cut(s) 35, 55, 66, 69, 864, 982, 1267
AclWI GGATC 1 cut(s) 262
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 4 cut(s) 587, 652, 901, 1337
AcuI CTGAAG 1 cut(s) 645
AcvI CACGTG 1 cut(s) 367
AfaI GTAC 2 cut(s) 968, 1247
AfiI CCNNNNNNNGG 4 cut(s) 48, 251, 266, 1402
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 2 cut(s) 478, 875
AjnI CCWGG 3 cut(s) 47, 115, 1395
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
Alw21I GWGCWC 2 cut(s) 301, 710
Alw26I GTCTC 1 cut(s) 511
AlwI GGATC 1 cut(s) 262
AlwNI CAGNNNCTG 1 cut(s) 1226
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 3 cut(s) 369, 643, 1143
ApeKI GCWGC 6 cut(s) 527, 790, 866, 984, 1226, 1346
ApoI RAATTY 4 cut(s) 587, 652, 901, 1337
Asp700I GAANNNNTTC 1 cut(s) 954
AspLEI GCGC 2 cut(s) 743, 1346
AsuHPI GGTGA 3 cut(s) 118, 249, 849
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 1 cut(s) 545
BalI TGGCCA 1 cut(s) 371
BanII GRGCYC 1 cut(s) 640
BauI CACGAG 1 cut(s) 264
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 2 cut(s) 301, 710
BbvI GCAGC 6 cut(s) 514, 802, 878, 996, 1213, 1333
BccI CCATC 5 cut(s) 245, 379, 626, 737, 914
BceAI ACGGC 3 cut(s) 22, 114, 1158
BciT130I CCWGG 3 cut(s) 49, 117, 1397
BcoDI GTCTC 1 cut(s) 511
BfaI CTAG 6 cut(s) 320, 336, 914, 932, 1208, 1257
BfoI RGCGCY 1 cut(s) 1347
BfuAI ACCTGC 1 cut(s) 868
BglII AGATCT 1 cut(s) 847
BmcAI AGTACT 1 cut(s) 968
Bme1390I CCNGG 3 cut(s) 49, 117, 1397
BmeT110I CYCGRG 1 cut(s) 545
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 3 cut(s) 49, 117, 1397
BpiI GAAGAC 1 cut(s) 556
BpmI CTGGAG 1 cut(s) 1331
Bpu10I CCTNAGC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 2 cut(s) 836, 1373
BsaAI YACGTR 2 cut(s) 367, 1181
BsaJI CCNNGG 3 cut(s) 47, 96, 1415
BsaWI WCCGGW 1 cut(s) 497
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 251, 266, 1402
Bse1I ACTGG 3 cut(s) 245, 357, 372
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 3 cut(s) 49, 117, 1397
BseDI CCNNGG 3 cut(s) 47, 96, 1415
BseGI GGATG 2 cut(s) 390, 885
BseLI CCNNNNNNNGG 4 cut(s) 48, 251, 266, 1402
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 5 cut(s) 517, 767, 930, 1233, 1352
BseNI ACTGG 3 cut(s) 245, 357, 372
BseRI GAGGAG 1 cut(s) 741
BseXI GCAGC 6 cut(s) 514, 802, 878, 996, 1213, 1333
BseYI CCCAGC 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 741
Bsh1285I CGRYCG 1 cut(s) 1271
BshFI GGCC 3 cut(s) 371, 645, 1145
BsiEI CGRYCG 1 cut(s) 1271
BsiHKAI GWGCWC 2 cut(s) 301, 710
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 1 cut(s) 498
BslFI GGGAC 2 cut(s) 196, 1142
BslI CCNNNNNNNGG 4 cut(s) 48, 251, 266, 1402
BsmAI GTCTC 1 cut(s) 511
BsmFI GGGAC 2 cut(s) 196, 1142
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 3 cut(s) 371, 645, 1145
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 3 cut(s) 301, 640, 710
Bsp1407I TGTACA 1 cut(s) 1245
Bsp143I GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
BspACI CCGC 7 cut(s) 35, 55, 66, 69, 864, 982, 1267
BspANI GGCC 3 cut(s) 371, 645, 1145
BspCNI CTCAG 5 cut(s) 516, 766, 931, 1232, 1353
BspFNI CGCG 1 cut(s) 741
BspHI TCATGA 1 cut(s) 1003
BspLI GGNNCC 1 cut(s) 53
BspMI ACCTGC 1 cut(s) 868
BspPI GGATC 1 cut(s) 262
BspQI GCTCTTC 1 cut(s) 876
BspT104I TTCGAA 1 cut(s) 958
BsrBI CCGCTC 1 cut(s) 1267
BsrDI GCAATG 1 cut(s) 483
BsrGI TGTACA 1 cut(s) 1245
BsrI ACTGG 3 cut(s) 245, 357, 372
BssECI CCNNGG 3 cut(s) 47, 96, 1415
BssMI GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 1415
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 49, 117, 1397
Bst4CI ACNGT 4 cut(s) 234, 468, 522, 1063
Bst6I CTCTTC 2 cut(s) 876, 1395
BstAPI GCANNNNNTGC 1 cut(s) 151
BstAUI TGTACA 1 cut(s) 1245
BstBAI YACGTR 2 cut(s) 367, 1181
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 8 cut(s) 66, 122, 490, 795, 1090, 1200, 1275, 1330
BstDEI CTNAG 6 cut(s) 102, 503, 753, 939, 1219, 1361
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 390, 885
BstFNI CGCG 1 cut(s) 741
BstH2I RGCGCY 1 cut(s) 1347
BstHHI GCGC 2 cut(s) 743, 1346
BstKTI GATC 6 cut(s) 257, 314, 362, 850, 1186, 1367
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
BstMCI CGRYCG 1 cut(s) 1271
BstMWI GCNNNNNNNGC 6 cut(s) 31, 121, 151, 397, 1208, 1235
BstNI CCWGG 3 cut(s) 49, 117, 1397
BstNSI RCATGY 1 cut(s) 492
BstSCI CCNGG 3 cut(s) 47, 115, 1395
BstUI CGCG 1 cut(s) 741
BstV1I GCAGC 6 cut(s) 514, 802, 878, 996, 1213, 1333
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 847
BstYI RGATCY 1 cut(s) 847
BsuRI GGCC 3 cut(s) 371, 645, 1145
BtgI CCRYGG 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 1347
BtsCI GGATG 2 cut(s) 390, 885
BtsIMutI CAGTG 2 cut(s) 350, 511
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 8 cut(s) 66, 122, 490, 795, 1090, 1200, 1275, 1330
CaiI CAGNNNCTG 1 cut(s) 1226
CciI TCATGA 1 cut(s) 1003
CfoI GCGC 2 cut(s) 743, 1346
CseI GACGC 1 cut(s) 833
Csp6I GTAC 2 cut(s) 967, 1246
CviAII CATG 6 cut(s) 151, 200, 489, 1004, 1024, 1162
CviQI GTAC 2 cut(s) 967, 1246
DdeI CTNAG 6 cut(s) 102, 503, 753, 939, 1219, 1361
DpnI GATC 6 cut(s) 256, 313, 361, 849, 1185, 1366
DpnII GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 2 cut(s) 876, 1395
EarI CTCTTC 2 cut(s) 876, 1395
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 1415
Eco24I GRGCYC 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 645
Eco72I CACGTG 1 cut(s) 367
Eco88I CYCGRG 1 cut(s) 545
EcoRI GAATTC 1 cut(s) 1337
EcoRII CCWGG 3 cut(s) 47, 115, 1395
EcoT14I CCWWGG 1 cut(s) 1415
EcoT38I GRGCYC 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 1415
FaeI CATG 6 cut(s) 154, 203, 492, 1007, 1027, 1165
FalI AAGNNNNNCTT 2 cut(s) 984, 1016
FaqI GGGAC 2 cut(s) 196, 1142
FatI CATG 6 cut(s) 150, 199, 488, 1003, 1023, 1161
FauI CCCGC 1 cut(s) 73
FblI GTMKAC 1 cut(s) 463
FokI GGATG 2 cut(s) 397, 872
FriOI GRGCYC 1 cut(s) 640
FspBI CTAG 6 cut(s) 320, 336, 914, 932, 1208, 1257
GlaI GCGC 2 cut(s) 742, 1345
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 1 cut(s) 1331
HaeII RGCGCY 1 cut(s) 1347
HaeIII GGCC 3 cut(s) 371, 645, 1145
HapII CCGG 1 cut(s) 498
HgaI GACGC 1 cut(s) 833
HhaI GCGC 2 cut(s) 743, 1346
Hin1II CATG 6 cut(s) 154, 203, 492, 1007, 1027, 1165
Hin6I GCGC 2 cut(s) 741, 1344
HinP1I GCGC 2 cut(s) 741, 1344
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 1086
HinfI GANTC 8 cut(s) 196, 501, 680, 725, 935, 1027, 1076, 1217
HpaII CCGG 1 cut(s) 498
HphI GGTGA 3 cut(s) 118, 249, 849
Hpy166II GTNNAC 3 cut(s) 16, 464, 598
Hpy188III TCNNGA 7 cut(s) 264, 343, 733, 932, 1004, 1109, 1221
Hpy8I GTNNAC 3 cut(s) 16, 464, 598
Hpy99I CGWCG 1 cut(s) 1217
HpyAV CCTTC 6 cut(s) 4, 93, 127, 154, 405, 656
HpyCH4III ACNGT 4 cut(s) 234, 468, 522, 1063
HpyCH4IV ACGT 3 cut(s) 325, 366, 1180
HpyCH4V TGCA 9 cut(s) 203, 408, 488, 492, 698, 790, 1092, 1202, 1229
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 121, 151, 397, 1208, 1235
HpyF3I CTNAG 6 cut(s) 102, 503, 753, 939, 1219, 1361
HpySE526I ACGT 3 cut(s) 325, 366, 1180
Hsp92II CATG 6 cut(s) 154, 203, 492, 1007, 1027, 1165
HspAI GCGC 2 cut(s) 741, 1344
Kzo9I GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 6 cut(s) 57, 142, 163, 1046, 1223, 1417
Lsp1109I GCAGC 6 cut(s) 514, 802, 878, 996, 1213, 1333
MaeI CTAG 6 cut(s) 320, 336, 914, 932, 1208, 1257
MaeII ACGT 3 cut(s) 325, 366, 1180
MaeIII GTNAC 3 cut(s) 855, 1096, 1405
MalI GATC 6 cut(s) 256, 313, 361, 849, 1185, 1366
MbiI CCGCTC 1 cut(s) 1267
MboI GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
MflI RGATCY 1 cut(s) 847
MhlI GDGCHC 3 cut(s) 301, 640, 710
MlsI TGGCCA 1 cut(s) 371
MluNI TGGCCA 1 cut(s) 371
MlyI GAGTC 4 cut(s) 205, 495, 719, 1211
MmeI TCCRAC 2 cut(s) 494, 1150
Mox20I TGGCCA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 954
MscI TGGCCA 1 cut(s) 371
MseI TTAA 3 cut(s) 591, 1083, 1139
MslI CAYNNNNRTG 3 cut(s) 155, 1008, 1110
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 1 cut(s) 498
MspR9I CCNGG 3 cut(s) 49, 117, 1397
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 3 cut(s) 49, 117, 1397
MvnI CGCG 1 cut(s) 741
MwoI GCNNNNNNNGC 6 cut(s) 31, 121, 151, 397, 1208, 1235
NdeII GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
NlaIII CATG 6 cut(s) 154, 203, 492, 1007, 1027, 1165
NlaIV GGNNCC 1 cut(s) 53
NmuCI GTSAC 2 cut(s) 855, 1096
NspI RCATGY 1 cut(s) 492
NspV TTCGAA 1 cut(s) 958
PaeI GCATGC 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 545
PagI TCATGA 1 cut(s) 1003
PaqCI CACCTGC 1 cut(s) 868
PciSI GCTCTTC 1 cut(s) 876
PctI GAATGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 954
PfeI GAWTC 4 cut(s) 680, 935, 1027, 1076
PflMI CCANNNNNTGG 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 1395
PleI GAGTC 4 cut(s) 204, 495, 719, 1211
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 4 cut(s) 204, 495, 719, 1211
Ppu21I YACGTR 2 cut(s) 367, 1181
Psp6I CCWGG 3 cut(s) 47, 115, 1395
PspCI CACGTG 1 cut(s) 367
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 3 cut(s) 47, 115, 1395
PspN4I GGNNCC 1 cut(s) 53
PspXI VCTCGAGB 1 cut(s) 545
PstNI CAGNNNCTG 1 cut(s) 1226
PsuI RGATCY 1 cut(s) 847
RsaI GTAC 2 cut(s) 968, 1247
RsaNI GTAC 2 cut(s) 967, 1246
RseI CAYNNNNRTG 3 cut(s) 155, 1008, 1110
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 3 cut(s) 591, 1083, 1139
Sau3AI GATC 6 cut(s) 254, 311, 359, 847, 1183, 1364
ScaI AGTACT 1 cut(s) 968
SchI GAGTC 4 cut(s) 205, 495, 719, 1211
ScrFI CCNGG 3 cut(s) 49, 117, 1397
SduI GDGCHC 3 cut(s) 301, 640, 710
Sfr274I CTCGAG 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 958
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 3 cut(s) 155, 1008, 1110
SmlI CTYRAG 3 cut(s) 545, 851, 1388
SmoI CTYRAG 3 cut(s) 545, 851, 1388
SphI GCATGC 1 cut(s) 492
SsiI CCGC 7 cut(s) 35, 55, 66, 69, 864, 982, 1267
SspI AATATT 1 cut(s) 1069
SspMI CTAG 6 cut(s) 320, 336, 914, 932, 1208, 1257
StyD4I CCNGG 3 cut(s) 47, 115, 1395
StyI CCWWGG 1 cut(s) 1415
TaaI ACNGT 4 cut(s) 234, 468, 522, 1063
TaiI ACGT 3 cut(s) 328, 369, 1183
TaqI TCGA 7 cut(s) 295, 310, 546, 576, 958, 1108, 1299
TatI WGTACW 2 cut(s) 966, 1245
TauI GCSGC 4 cut(s) 37, 71, 866, 984
TfiI GAWTC 4 cut(s) 680, 935, 1027, 1076
Tru1I TTAA 3 cut(s) 591, 1083, 1139
Tru9I TTAA 3 cut(s) 591, 1083, 1139
TscAI CASTG 2 cut(s) 357, 511
TseFI GTSAC 2 cut(s) 855, 1096
TseI GCWGC 6 cut(s) 527, 790, 866, 984, 1226, 1346
Tsp45I GTSAC 2 cut(s) 855, 1096
TspDTI ATGAA 7 cut(s) 173, 468, 992, 1020, 1040, 1178, 1350
TspRI CASTG 2 cut(s) 357, 511
Van91I CCANNNNNTGG 1 cut(s) 251
XapI RAATTY 4 cut(s) 587, 652, 901, 1337
XbaI TCTAGA 1 cut(s) 931
XceI RCATGY 1 cut(s) 492
XhoI CTCGAG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 463
XmnI GAANNNNTTC 1 cut(s) 954
XspI CTAG 6 cut(s) 320, 336, 914, 932, 1208, 1257
ZrmI AGTACT 1 cut(s) 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.