Rmu_sc0001407.1_g000012

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001407.1
OG0012565
Physical Location & Seq
Forward (+)
74558 .. 79253
4696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001407.1_g000012.1.cds

Sequence Viewer

Length: 1260 bp
atgccaaaagcgacgtcgcagcagttaggcagtccagcgcggggctcagagtcggagacatctccgtcggtttcttcagaagaatctaatataatgggtcggaatactagaaaaggaaccccccaacgagcggagaagaaggagaataaacctaagacgccgccgtccaaggaggaggtattgtcccgcctcaccaagcccaaacccaaaccaaagtctaccaggcctgttgccacgccgccgaagaagacaaacaagtcgaacgagaaggaagtagagcattcgttgcccatgaaggagctttccaatacccaagttaacaaaatgcttgagcaagctgcattgggtcattcagtgaagtccactcccttaagtaatacgcaatatgattttccgattgatggctcaccctcacgtggtcaagcaaagtcatctgcaatcattcgagcacaggaagtccgatcaaaactagaacctctattccctagcttcgtgaaatcactcctccgatctcatgtagctagttataaagataatgcagaaacaggtgcagtggcttgtaatgagtctaacaggaaacgcacaaagtctcctcctgaagttgttcaaaagaagaaaaagattgacttgccagttgaacactctgaaatcgatagtgaggaagttgattcagaagttgtggaaagctccaggttacctgggacaactcttcaatttgaggacatcaaaagttttgagaacttcaacatttttgtaaatggattgcttgtagattctgagttccccgaagatatacgaaggaagtactacaagctctgccgcagtcagaatgcatttcttcatgaaaatgttatgaagggaacatattataaattgattgtaggagctatatttgaaactgtacttattgtcgacgccattaaagcttgccagattaccacgactcaagatgattttgtgtgttgggacaaatacttgagggcctttgagttgttgggtatgaatgttggatttatacgtgcgcggttatgccggcttgcaaaccttgcctttgactcagaagatgctatggatgcgaagacatataaagaagctagaaaagaacgagctcgagcaggactaggactaagaagtatcgagacaaagattcaggagttgagggaggcatgccatgaatttagtagtgctatcgagagtctgagatccaaagctgaagtctacaatcaaaggttccaggaagaggttactgctccttggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

419

Amino Acids

47.17

Weight (kDa)

9.04

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 528, 870
AasI GACNNNNNNGTC 3 cut(s) 64, 163, 256
AatII GACGTC 1 cut(s) 17
AccBSI CCGCTC 1 cut(s) 131
AccI GTMKAC 3 cut(s) 218, 912, 1218
AccII CGCG 2 cut(s) 40, 1024
AciI CCGC 7 cut(s) 40, 131, 161, 187, 239, 820, 1024
AclWI GGATC 1 cut(s) 1197
AcsI RAATTY 1 cut(s) 1175
AcuI CTGAAG 3 cut(s) 60, 618, 1233
AcvI CACGTG 1 cut(s) 416
AcyI GRCGYC 3 cut(s) 14, 158, 915
AfaI GTAC 2 cut(s) 806, 903
AfiI CCNNNNNNNGG 5 cut(s) 41, 130, 401, 416, 1240
AflII CTTAAG 1 cut(s) 370
AgsI TTSAA 5 cut(s) 608, 638, 713, 745, 896
AjnI CCWGG 4 cut(s) 221, 689, 697, 1233
AloI GAACNNNNNNTCC 2 cut(s) 465, 497
Alw21I GWGCWC 2 cut(s) 451, 1111
Alw26I GTCTC 3 cut(s) 50, 594, 1133
AlwI GGATC 1 cut(s) 1197
Ama87I CYCGRG 1 cut(s) 1110
AoxI GGCC 2 cut(s) 224, 981
ApeKI GCWGC 2 cut(s) 19, 338
ApoI RAATTY 1 cut(s) 1175
ArsI GACNNNNNNTTYG 3 cut(s) 31, 1200, 1232
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 2 cut(s) 40, 1024
AspS9I GGNCC 1 cut(s) 981
AsuHPI GGTGA 2 cut(s) 184, 399
AvaI CYCGRG 1 cut(s) 1110
BanII GRGCYC 2 cut(s) 47, 1111
BbrPI CACGTG 1 cut(s) 416
BbsI GAAGAC 2 cut(s) 254, 1085
Bbv12I GWGCWC 2 cut(s) 451, 1111
BbvI GCAGC 2 cut(s) 31, 325
BccI CCATC 1 cut(s) 395
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 4 cut(s) 223, 691, 699, 1235
BcoDI GTCTC 3 cut(s) 50, 594, 1133
BfaI CTAG 6 cut(s) 108, 470, 486, 522, 1095, 1121
BfrI CTTAAG 1 cut(s) 370
BisI GCNGC 5 cut(s) 20, 161, 239, 339, 820
BlsI GCNGC 5 cut(s) 21, 162, 240, 340, 821
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 4 cut(s) 223, 691, 699, 1235
BmeT110I CYCGRG 1 cut(s) 1110
BmgT120I GGNCC 1 cut(s) 981
BmiI GGNNCC 2 cut(s) 118, 1232
BmrFI CCNGG 4 cut(s) 223, 691, 699, 1235
BmsI GCATC 2 cut(s) 1054, 1063
BpiI GAAGAC 2 cut(s) 254, 1085
BpmI CTGGAG 1 cut(s) 673
BpuEI CTTGAG 3 cut(s) 350, 930, 997
Bsa29I ATCGAT 1 cut(s) 651
BsaAI YACGTR 2 cut(s) 416, 1019
BsaHI GRCGYC 3 cut(s) 14, 158, 915
BsaJI CCNNGG 3 cut(s) 168, 698, 1253
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 574, 604
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 130, 401, 416, 1240
Bse118I RCCGGY 1 cut(s) 1032
Bse1I ACTGG 1 cut(s) 632
BseBI CCWGG 4 cut(s) 223, 691, 699, 1235
BseCI ATCGAT 1 cut(s) 651
BseDI CCNNGG 3 cut(s) 168, 698, 1253
BseGI GGATG 1 cut(s) 1078
BseLI CCNNNNNNNGG 5 cut(s) 41, 130, 401, 416, 1240
BseMII CTCAG 4 cut(s) 60, 768, 1071, 1190
BseNI ACTGG 1 cut(s) 632
BseRI GAGGAG 3 cut(s) 188, 494, 582
BseXI GCAGC 2 cut(s) 31, 325
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 2 cut(s) 40, 1024
BshFI GGCC 2 cut(s) 226, 983
BshVI ATCGAT 1 cut(s) 651
BsiHKAI GWGCWC 2 cut(s) 451, 1111
BsiHKCI CYCGRG 1 cut(s) 1110
BsiSI CCGG 1 cut(s) 1033
BslFI GGGAC 3 cut(s) 169, 715, 980
BslI CCNNNNNNNGG 5 cut(s) 41, 130, 401, 416, 1240
BsmAI GTCTC 3 cut(s) 50, 594, 1133
BsmFI GGGAC 3 cut(s) 169, 715, 980
BsmI GAATGC 2 cut(s) 280, 835
BsnI GGCC 2 cut(s) 226, 983
BsoBI CYCGRG 1 cut(s) 1110
Bsp1286I GDGCHC 3 cut(s) 47, 451, 1111
Bsp143I GATC 3 cut(s) 461, 509, 1202
BspACI CCGC 7 cut(s) 40, 131, 161, 187, 239, 820, 1024
BspANI GGCC 2 cut(s) 226, 983
BspCNI CTCAG 4 cut(s) 59, 769, 1070, 1191
BspDI ATCGAT 1 cut(s) 651
BspFNI CGCG 2 cut(s) 40, 1024
BspHI TCATGA 1 cut(s) 841
BspLI GGNNCC 2 cut(s) 118, 1232
BspPI GGATC 1 cut(s) 1197
BspTI CTTAAG 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 131
BsrFI RCCGGY 1 cut(s) 1032
BsrI ACTGG 1 cut(s) 632
BssAI RCCGGY 1 cut(s) 1032
BssECI CCNNGG 3 cut(s) 168, 698, 1253
BssMI GATC 3 cut(s) 461, 509, 1202
BssNI GRCGYC 3 cut(s) 14, 158, 915
BssT1I CCWWGG 2 cut(s) 168, 1253
Bst2UI CCWGG 4 cut(s) 223, 691, 699, 1235
Bst4CI ACNGT 1 cut(s) 901
Bst6I CTCTTC 2 cut(s) 714, 1233
BstACI GRCGYC 3 cut(s) 14, 158, 915
BstAFI CTTAAG 1 cut(s) 370
BstAPI GCANNNNNTGC 2 cut(s) 286, 1046
BstBAI YACGTR 2 cut(s) 416, 1019
BstC8I GCNNGC 5 cut(s) 336, 928, 1034, 1038, 1168
BstDEI CTNAG 6 cut(s) 46, 153, 777, 1057, 1127, 1199
BstEII GGTNACC 1 cut(s) 693
BstF5I GGATG 1 cut(s) 1078
BstFNI CGCG 2 cut(s) 40, 1024
BstHHI GCGC 2 cut(s) 40, 1024
BstKTI GATC 3 cut(s) 464, 512, 1205
BstMAI GTCTC 3 cut(s) 50, 594, 1133
BstMBI GATC 3 cut(s) 461, 509, 1202
BstMWI GCNNNNNNNGC 4 cut(s) 286, 923, 1046, 1073
BstNI CCWGG 4 cut(s) 223, 691, 699, 1235
BstNSI RCATGY 1 cut(s) 1170
BstPI GGTNACC 1 cut(s) 693
BstSCI CCNGG 4 cut(s) 221, 689, 697, 1233
BstUI CGCG 2 cut(s) 40, 1024
BstV1I GCAGC 2 cut(s) 31, 325
BstV2I GAAGAC 2 cut(s) 254, 1085
BstX2I RGATCY 1 cut(s) 1202
BstYI RGATCY 1 cut(s) 1202
Bsu15I ATCGAT 1 cut(s) 651
BsuRI GGCC 2 cut(s) 226, 983
BsuTUI ATCGAT 1 cut(s) 651
BtsCI GGATG 1 cut(s) 1078
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 2 cut(s) 360, 558
Cac8I GCNNGC 5 cut(s) 336, 928, 1034, 1038, 1168
CciI TCATGA 1 cut(s) 841
CfoI GCGC 2 cut(s) 40, 1024
Cfr10I RCCGGY 1 cut(s) 1032
Cfr13I GGNCC 1 cut(s) 981
ClaI ATCGAT 1 cut(s) 651
CseI GACGC 2 cut(s) 166, 923
Csp6I GTAC 2 cut(s) 805, 902
CviAII CATG 5 cut(s) 292, 515, 842, 1167, 1172
CviQI GTAC 2 cut(s) 805, 902
DdeI CTNAG 6 cut(s) 46, 153, 777, 1057, 1127, 1199
DpnI GATC 3 cut(s) 463, 511, 1204
DpnII GATC 3 cut(s) 461, 509, 1202
DrdI GACNNNNNNGTC 3 cut(s) 64, 163, 256
DseDI GACNNNNNNGTC 3 cut(s) 64, 163, 256
Eam1104I CTCTTC 2 cut(s) 714, 1233
EarI CTCTTC 2 cut(s) 714, 1233
Ecl136II GAGCTC 1 cut(s) 1109
Eco130I CCWWGG 2 cut(s) 168, 1253
Eco147I AGGCCT 1 cut(s) 226
Eco24I GRGCYC 2 cut(s) 47, 1111
Eco53kI GAGCTC 1 cut(s) 1109
Eco57I CTGAAG 3 cut(s) 60, 618, 1233
Eco72I CACGTG 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 1110
Eco91I GGTNACC 1 cut(s) 693
EcoICRI GAGCTC 1 cut(s) 1109
EcoO109I RGGNCCY 1 cut(s) 981
EcoO65I GGTNACC 1 cut(s) 693
EcoRII CCWGG 4 cut(s) 221, 689, 697, 1233
EcoT14I CCWWGG 2 cut(s) 168, 1253
EcoT22I ATGCAT 1 cut(s) 835
EcoT38I GRGCYC 2 cut(s) 47, 1111
ErhI CCWWGG 2 cut(s) 168, 1253
FaeI CATG 5 cut(s) 295, 518, 845, 1170, 1175
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 3 cut(s) 169, 715, 980
FatI CATG 5 cut(s) 291, 514, 841, 1166, 1171
FauI CCCGC 2 cut(s) 33, 194
FblI GTMKAC 3 cut(s) 218, 912, 1218
Fnu4HI GCNGC 5 cut(s) 20, 161, 239, 339, 820
FokI GGATG 1 cut(s) 1085
FriOI GRGCYC 2 cut(s) 47, 1111
Fsp4HI GCNGC 5 cut(s) 20, 161, 239, 339, 820
FspBI CTAG 6 cut(s) 108, 470, 486, 522, 1095, 1121
GlaI GCGC 2 cut(s) 39, 1023
GluI GCNGC 5 cut(s) 20, 161, 239, 339, 820
GsuI CTGGAG 1 cut(s) 673
HaeIII GGCC 2 cut(s) 226, 983
HapII CCGG 1 cut(s) 1033
HgaI GACGC 2 cut(s) 166, 923
HhaI GCGC 2 cut(s) 40, 1024
Hin1I GRCGYC 3 cut(s) 14, 158, 915
Hin1II CATG 5 cut(s) 295, 518, 845, 1170, 1175
Hin6I GCGC 2 cut(s) 38, 1022
HinP1I GCGC 2 cut(s) 38, 1022
HincII GTYRAC 2 cut(s) 319, 913
HindII GTYRAC 2 cut(s) 319, 913
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 9 cut(s) 50, 83, 566, 668, 773, 943, 1055, 1147, 1195
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 1 cut(s) 1033
HphI GGTGA 2 cut(s) 184, 399
Hpy166II GTNNAC 5 cut(s) 219, 319, 363, 913, 1219
Hpy188III TCNNGA 7 cut(s) 493, 596, 842, 947, 1138, 1151, 1192
Hpy8I GTNNAC 5 cut(s) 219, 319, 363, 913, 1219
Hpy99I CGWCG 4 cut(s) 16, 19, 70, 917
HpyAV CCTTC 5 cut(s) 133, 262, 289, 792, 850
HpyCH4III ACNGT 1 cut(s) 901
HpyCH4IV ACGT 3 cut(s) 14, 415, 1018
HpyCH4V TGCA 6 cut(s) 341, 437, 539, 551, 833, 1040
HpyF10VI GCNNNNNNNGC 4 cut(s) 286, 923, 1046, 1073
HpyF3I CTNAG 6 cut(s) 46, 153, 777, 1057, 1127, 1199
HpySE526I ACGT 3 cut(s) 14, 415, 1018
Hsp92I GRCGYC 3 cut(s) 14, 158, 915
Hsp92II CATG 5 cut(s) 295, 518, 845, 1170, 1175
HspAI GCGC 2 cut(s) 38, 1022
KroI GCCGGC 1 cut(s) 1032
KroNI GCCGGC 1 cut(s) 1034
KspAI GTTAAC 1 cut(s) 319
Kzo9I GATC 3 cut(s) 461, 509, 1202
LmnI GCTCC 4 cut(s) 298, 692, 884, 1255
Lsp1109I GCAGC 2 cut(s) 31, 325
LweI GCATC 2 cut(s) 1054, 1063
MaeI CTAG 6 cut(s) 108, 470, 486, 522, 1095, 1121
MaeII ACGT 3 cut(s) 14, 415, 1018
MaeIII GTNAC 2 cut(s) 693, 1243
MalI GATC 3 cut(s) 463, 511, 1204
MbiI CCGCTC 1 cut(s) 131
MboI GATC 3 cut(s) 461, 509, 1202
MflI RGATCY 1 cut(s) 1202
MhlI GDGCHC 3 cut(s) 47, 451, 1111
MluCI AATT 3 cut(s) 713, 872, 1175
MlyI GAGTC 5 cut(s) 59, 575, 937, 1049, 1204
MmeI TCCRAC 3 cut(s) 33, 80, 988
Mph1103I ATGCAT 1 cut(s) 835
MroNI GCCGGC 1 cut(s) 1032
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 3 cut(s) 318, 371, 921
MslI CAYNNNNRTG 1 cut(s) 846
MspCI CTTAAG 1 cut(s) 370
MspI CCGG 1 cut(s) 1033
MspR9I CCNGG 4 cut(s) 223, 691, 699, 1235
Mva1269I GAATGC 2 cut(s) 280, 835
MvaI CCWGG 4 cut(s) 223, 691, 699, 1235
MvnI CGCG 2 cut(s) 40, 1024
MwoI GCNNNNNNNGC 4 cut(s) 286, 923, 1046, 1073
NaeI GCCGGC 1 cut(s) 1034
NdeII GATC 3 cut(s) 461, 509, 1202
NgoMIV GCCGGC 1 cut(s) 1032
NlaIII CATG 5 cut(s) 295, 518, 845, 1170, 1175
NlaIV GGNNCC 2 cut(s) 118, 1232
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 1 cut(s) 1170
PaeI GCATGC 1 cut(s) 1170
PaeR7I CTCGAG 1 cut(s) 1110
PagI TCATGA 1 cut(s) 841
PceI AGGCCT 1 cut(s) 226
PctI GAATGC 2 cut(s) 280, 835
PdiI GCCGGC 1 cut(s) 1034
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 4 cut(s) 83, 668, 773, 1147
PfoI TCCNGGA 1 cut(s) 1233
PkrI GCNGC 5 cut(s) 21, 162, 240, 340, 821
PleI GAGTC 5 cut(s) 58, 574, 937, 1049, 1203
PmaCI CACGTG 1 cut(s) 416
PmlI CACGTG 1 cut(s) 416
PpsI GAGTC 5 cut(s) 58, 574, 937, 1049, 1203
Ppu21I YACGTR 2 cut(s) 416, 1019
PsiI TTATAA 2 cut(s) 528, 870
Psp124BI GAGCTC 1 cut(s) 1111
Psp6I CCWGG 4 cut(s) 221, 689, 697, 1233
PspCI CACGTG 1 cut(s) 416
PspEI GGTNACC 1 cut(s) 693
PspGI CCWGG 4 cut(s) 221, 689, 697, 1233
PspN4I GGNNCC 2 cut(s) 118, 1232
PspPI GGNCC 1 cut(s) 981
PspXI VCTCGAGB 1 cut(s) 1110
PsuI RGATCY 1 cut(s) 1202
RsaI GTAC 2 cut(s) 806, 903
RsaNI GTAC 2 cut(s) 805, 902
RseI CAYNNNNRTG 1 cut(s) 846
SacI GAGCTC 1 cut(s) 1111
SalI GTCGAC 1 cut(s) 911
SaqAI TTAA 3 cut(s) 318, 371, 921
SatI GCNGC 5 cut(s) 20, 161, 239, 339, 820
Sau3AI GATC 3 cut(s) 461, 509, 1202
Sau96I GGNCC 1 cut(s) 981
ScaI AGTACT 1 cut(s) 806
SchI GAGTC 5 cut(s) 59, 575, 937, 1049, 1204
ScrFI CCNGG 4 cut(s) 223, 691, 699, 1235
SduI GDGCHC 3 cut(s) 47, 451, 1111
SfaNI GCATC 2 cut(s) 1054, 1063
Sfr274I CTCGAG 1 cut(s) 1110
SlaI CTCGAG 1 cut(s) 1110
SmiMI CAYNNNNRTG 1 cut(s) 846
SmlI CTYRAG 5 cut(s) 329, 370, 945, 976, 1110
SmoI CTYRAG 5 cut(s) 329, 370, 945, 976, 1110
SphI GCATGC 1 cut(s) 1170
Sse9I AATT 3 cut(s) 713, 872, 1175
SseBI AGGCCT 1 cut(s) 226
SsiI CCGC 7 cut(s) 40, 131, 161, 187, 239, 820, 1024
SspMI CTAG 6 cut(s) 108, 470, 486, 522, 1095, 1121
SstI GAGCTC 1 cut(s) 1111
StuI AGGCCT 1 cut(s) 226
StyD4I CCNGG 4 cut(s) 221, 689, 697, 1233
StyI CCWWGG 2 cut(s) 168, 1253
TaaI ACNGT 1 cut(s) 901
TaiI ACGT 3 cut(s) 17, 418, 1021
TaqI TCGA 7 cut(s) 260, 445, 651, 912, 1111, 1137, 1191
TasI AATT 3 cut(s) 713, 872, 1175
TatI WGTACW 2 cut(s) 804, 901
TauI GCSGC 3 cut(s) 163, 241, 822
TfiI GAWTC 4 cut(s) 83, 668, 773, 1147
Tru1I TTAA 3 cut(s) 318, 371, 921
Tru9I TTAA 3 cut(s) 318, 371, 921
TscAI CASTG 2 cut(s) 360, 558
TseI GCWGC 2 cut(s) 19, 338
TspDTI ATGAA 6 cut(s) 308, 830, 858, 869, 1016, 1188
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 2 cut(s) 360, 558
Vha464I CTTAAG 1 cut(s) 370
XapI RAATTY 1 cut(s) 1175
XceI RCATGY 1 cut(s) 1170
XhoI CTCGAG 1 cut(s) 1110
XmiI GTMKAC 3 cut(s) 218, 912, 1218
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 6 cut(s) 108, 470, 486, 522, 1095, 1121
ZraI GACGTC 1 cut(s) 15
ZrmI AGTACT 1 cut(s) 806
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.