pycom02g13730

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012565
Physical Location & Seq
Forward (+)
10323403 .. 10324152
750 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13730.1

Sequence Viewer

Length: 750 bp
ATGTTACACTCAGATAAAGATAATGCAGAAACGAGCACTACGCCTTGTAATGACTCCATCAGGAAACGCGCACATTCTCCTCAGTTAGTAGTTCCAAAGAAGAAAAAGATTGGTTTGCAGAGAGCCGAACAATCTGAAAATGATAGTGAGGAGGCTGGTTCGGAAGTTTCAGAAGATCTCAAGTCACCTGCCGCAGCTCTTCAATTTGAGGACATCCGAAGTTACGAGAATTTTCACATTCTAGTAGATGGGTTGTTTCTAGATTCTGAGGTCCCCGAAGATGTTCGAAAGAAGTACTACAAGCTCTGCCGCAGCCAGAAAGCGTTTCTTCATGAAAATGTTATCAAGGGCATGAATCCTAATTTGATTATAGGAGCTATATCTGAAACTGTAAATATTGCCGATTCCATTAAAGCTTGCAAGGTCACTACACCTCGAGATGATTTTGTGTCTTGGGACAAGTATTTAACGGCCTTTGAGTTGTTGGGCATGAATGTTGGATTTTTACGTGATCGGATATGTCGGCTTGCAAACCTAGCCGACGACTCAGGAGCTGCATTGGAGGCAAAAATGTACAAAGAAGCTAGAATTGAGCGGTCGCAGGCAGGAATAGGAATAAGAAATATCGAAGTGAAGCTGGAGGAAATGAGGGAGGCGTGCGATGAATTCGGCGCTGCCATTGAGAGCCTGAGATCAAAAGCTGAAATCAACACGCTTAAGTTCCAGGAAGAGGTTACTGCTCCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

250

Amino Acids

28.0

Weight (kDa)

5.24

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 196
Acc36I ACCTGC 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 595
AccII CGCG 1 cut(s) 69
AciI CCGC 3 cut(s) 192, 310, 595
AcsI RAATTY 2 cut(s) 229, 665
AfaI GTAC 2 cut(s) 296, 575
AfiI CCNNNNNNNGG 1 cut(s) 730
AflII CTTAAG 1 cut(s) 716
AgsI TTSAA 1 cut(s) 203
AjnI CCWGG 1 cut(s) 723
AluBI AGCT 8 cut(s) 197, 304, 377, 416, 554, 584, 637, 701
AluI AGCT 8 cut(s) 197, 304, 377, 416, 554, 584, 637, 701
Alw21I GWGCWC 1 cut(s) 38
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 435
AoxI GGCC 1 cut(s) 471
ApeKI GCWGC 4 cut(s) 194, 312, 554, 674
ApoI RAATTY 2 cut(s) 229, 665
Asp700I GAANNNNTTC 1 cut(s) 282
AspLEI GCGC 2 cut(s) 71, 674
AspS9I GGNCC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 177
AsuII TTCGAA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 435
AvaII GGWCC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 4 cut(s) 206, 324, 541, 661
BccI CCATC 2 cut(s) 65, 242
BceAI ACGGC 1 cut(s) 486
BciT130I CCWGG 1 cut(s) 725
BfaI CTAG 4 cut(s) 242, 260, 536, 585
BfoI RGCGCY 1 cut(s) 675
BfrI CTTAAG 1 cut(s) 716
BfuAI ACCTGC 1 cut(s) 196
BglII AGATCT 1 cut(s) 175
BisI GCNGC 6 cut(s) 192, 195, 310, 313, 555, 675
BlsI GCNGC 6 cut(s) 193, 196, 311, 314, 556, 676
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 1 cut(s) 725
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 659
Bpu14I TTCGAA 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 743
Bsc4I CCNNNNNNNGG 1 cut(s) 730
BseBI CCWGG 1 cut(s) 725
BseDI CCNNGG 1 cut(s) 743
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 730
BseMII CTCAG 5 cut(s) 24, 95, 258, 561, 680
BseRI GAGGAG 2 cut(s) 69, 164
BseXI GCAGC 4 cut(s) 206, 324, 541, 661
Bsh1236I CGCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 599
BshFI GGCC 1 cut(s) 473
BsiEI CGRYCG 1 cut(s) 599
BsiHKAI GWGCWC 1 cut(s) 38
BsiHKCI CYCGRG 1 cut(s) 435
BslFI GGGAC 2 cut(s) 257, 470
BslI CCNNNNNNNGG 1 cut(s) 730
BsmFI GGGAC 2 cut(s) 257, 470
BsnI GGCC 1 cut(s) 473
BsoBI CYCGRG 1 cut(s) 435
Bsp119I TTCGAA 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 3 cut(s) 175, 511, 692
BspACI CCGC 3 cut(s) 192, 310, 595
BspANI GGCC 1 cut(s) 473
BspCNI CTCAG 5 cut(s) 23, 94, 259, 560, 681
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 273
BspMI ACCTGC 1 cut(s) 196
BspQI GCTCTTC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 286
BspTI CTTAAG 1 cut(s) 716
BsrBI CCGCTC 1 cut(s) 595
BsrGI TGTACA 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 743
BssMI GATC 3 cut(s) 175, 511, 692
BssT1I CCWWGG 1 cut(s) 743
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 2 cut(s) 204, 723
BstAFI CTTAAG 1 cut(s) 716
BstAUI TGTACA 1 cut(s) 573
BstBAI YACGTR 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 4 cut(s) 418, 528, 603, 658
BstDEI CTNAG 5 cut(s) 10, 81, 267, 547, 689
BstF5I GGATG 1 cut(s) 213
BstFNI CGCG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 675
BstHHI GCGC 2 cut(s) 71, 674
BstKTI GATC 3 cut(s) 178, 514, 695
BstMBI GATC 3 cut(s) 175, 511, 692
BstMCI CGRYCG 1 cut(s) 599
BstMWI GCNNNNNNNGC 2 cut(s) 536, 563
BstNI CCWGG 1 cut(s) 725
BstSCI CCNGG 1 cut(s) 723
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 4 cut(s) 206, 324, 541, 661
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 1 cut(s) 473
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 1 cut(s) 213
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 4 cut(s) 418, 528, 603, 658
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 331
CfoI GCGC 2 cut(s) 71, 674
Cfr13I GGNCC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 295, 574
CviAII CATG 3 cut(s) 332, 352, 490
CviQI GTAC 2 cut(s) 295, 574
DdeI CTNAG 5 cut(s) 10, 81, 267, 547, 689
DpnI GATC 3 cut(s) 177, 513, 694
DpnII GATC 3 cut(s) 175, 511, 692
Eam1104I CTCTTC 2 cut(s) 204, 723
EarI CTCTTC 2 cut(s) 204, 723
Eco130I CCWWGG 1 cut(s) 743
Eco47I GGWCC 1 cut(s) 271
Eco88I CYCGRG 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 665
EcoRII CCWGG 1 cut(s) 723
EcoT14I CCWWGG 1 cut(s) 743
ErhI CCWWGG 1 cut(s) 743
FaeI CATG 3 cut(s) 335, 355, 493
FaiI YATR 6 cut(s) 333, 353, 371, 380, 491, 520
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 2 cut(s) 257, 470
FatI CATG 3 cut(s) 331, 351, 489
Fnu4HI GCNGC 6 cut(s) 192, 195, 310, 313, 555, 675
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 6 cut(s) 192, 195, 310, 313, 555, 675
FspBI CTAG 4 cut(s) 242, 260, 536, 585
GlaI GCGC 2 cut(s) 70, 673
GluI GCNGC 6 cut(s) 192, 195, 310, 313, 555, 675
GsuI CTGGAG 1 cut(s) 659
HaeII RGCGCY 1 cut(s) 675
HaeIII GGCC 1 cut(s) 473
HhaI GCGC 2 cut(s) 71, 674
Hin1II CATG 3 cut(s) 335, 355, 493
Hin6I GCGC 2 cut(s) 69, 672
HinP1I GCGC 2 cut(s) 69, 672
HindIII AAGCTT 1 cut(s) 414
HinfI GANTC 5 cut(s) 53, 263, 355, 404, 545
HphI GGTGA 1 cut(s) 177
Hpy188I TCNGA 8 cut(s) 13, 136, 163, 172, 218, 268, 385, 516
Hpy188III TCNNGA 5 cut(s) 61, 260, 332, 437, 549
Hpy99I CGWCG 1 cut(s) 545
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 508
HpyCH4V TGCA 5 cut(s) 26, 118, 420, 530, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 563
HpyF3I CTNAG 5 cut(s) 10, 81, 267, 547, 689
HpySE526I ACGT 1 cut(s) 508
Hsp92II CATG 3 cut(s) 335, 355, 493
HspAI GCGC 2 cut(s) 69, 672
Kzo9I GATC 3 cut(s) 175, 511, 692
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 3 cut(s) 374, 551, 745
Lsp1109I GCAGC 4 cut(s) 206, 324, 541, 661
MaeI CTAG 4 cut(s) 242, 260, 536, 585
MaeII ACGT 1 cut(s) 508
MaeIII GTNAC 5 cut(s) 3, 183, 221, 424, 733
MalI GATC 3 cut(s) 177, 513, 694
MbiI CCGCTC 1 cut(s) 595
MboI GATC 3 cut(s) 175, 511, 692
MboII GAAGA 6 cut(s) 112, 185, 191, 290, 320, 740
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 5 cut(s) 203, 229, 361, 588, 665
MlyI GAGTC 2 cut(s) 47, 539
MmeI TCCRAC 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 3 cut(s) 411, 467, 717
MslI CAYNNNNRTG 1 cut(s) 336
MspCI CTTAAG 1 cut(s) 716
MspR9I CCNGG 1 cut(s) 725
MvaI CCWGG 1 cut(s) 725
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 536, 563
NdeII GATC 3 cut(s) 175, 511, 692
NlaIII CATG 3 cut(s) 335, 355, 493
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 2 cut(s) 183, 424
NspV TTCGAA 1 cut(s) 286
PaeR7I CTCGAG 1 cut(s) 435
PagI TCATGA 1 cut(s) 331
PaqCI CACCTGC 1 cut(s) 196
PciSI GCTCTTC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 3 cut(s) 263, 355, 404
PfoI TCCNGGA 1 cut(s) 723
PkrI GCNGC 6 cut(s) 193, 196, 311, 314, 556, 676
PleI GAGTC 2 cut(s) 47, 539
PpsI GAGTC 2 cut(s) 47, 539
Ppu21I YACGTR 1 cut(s) 509
PpuMI RGGWCCY 1 cut(s) 271
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 723
PspGI CCWGG 1 cut(s) 723
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 1 cut(s) 271
PspPPI RGGWCCY 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 2 cut(s) 296, 575
RsaNI GTAC 2 cut(s) 295, 574
RseI CAYNNNNRTG 1 cut(s) 336
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 3 cut(s) 411, 467, 717
SatI GCNGC 6 cut(s) 192, 195, 310, 313, 555, 675
Sau3AI GATC 3 cut(s) 175, 511, 692
Sau96I GGNCC 1 cut(s) 271
ScaI AGTACT 1 cut(s) 296
SchI GAGTC 2 cut(s) 47, 539
ScrFI CCNGG 1 cut(s) 725
SduI GDGCHC 1 cut(s) 38
Sfr274I CTCGAG 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 286
SinI GGWCC 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 3 cut(s) 179, 435, 716
SmoI CTYRAG 3 cut(s) 179, 435, 716
Sse9I AATT 5 cut(s) 203, 229, 361, 588, 665
SsiI CCGC 3 cut(s) 192, 310, 595
SspI AATATT 1 cut(s) 397
SspMI CTAG 4 cut(s) 242, 260, 536, 585
StyD4I CCNGG 1 cut(s) 723
StyI CCWWGG 1 cut(s) 743
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 511
TaqI TCGA 3 cut(s) 286, 436, 627
TasI AATT 5 cut(s) 203, 229, 361, 588, 665
TatI WGTACW 2 cut(s) 294, 573
TauI GCSGC 2 cut(s) 194, 312
TfiI GAWTC 3 cut(s) 263, 355, 404
Tru1I TTAA 3 cut(s) 411, 467, 717
Tru9I TTAA 3 cut(s) 411, 467, 717
TseFI GTSAC 2 cut(s) 183, 424
TseI GCWGC 4 cut(s) 194, 312, 554, 674
Tsp45I GTSAC 2 cut(s) 183, 424
TspDTI ATGAA 5 cut(s) 320, 348, 368, 506, 678
Vha464I CTTAAG 1 cut(s) 716
VpaK11BI GGWCC 1 cut(s) 271
XapI RAATTY 2 cut(s) 229, 665
XbaI TCTAGA 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 4 cut(s) 242, 260, 536, 585
ZrmI AGTACT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.