Rh2CG182500

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0012565
Physical Location & Seq
Forward (+)
16863028 .. 16868055
5028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG182500.1

Sequence Viewer

Length: 1278 bp
ATGAAGGAGCTTTCCAATACCCAAGTAAACAAAATGCTTGAGCAAGCTGCATTGGGTCATTCAGTGAAGTCCACTCCCTTAAGTAATACGCAATATGATTTTCCGATTGATGGCTCACCCTCACGTGGTCAAGCAAAGTCATCTGCAATCATTCGAGCACAGGAAGTCCGATCAAAACTAGAACCTCTATTCCCTAGCTTCGTGAAATCACTCCTCCGATCTCATGTAGCTAGTTGTTTTTGGATGGGACTTCCTTCGCTGTTTTGCAAATCACACTTACCAGAAAAAGATATTACAATGACTCTGGAAGATGAAGGTGGAAGACAGTTTCAATCTAAGTACATTGCATGCAAAACCGGACTGAGTGCTGGTTGGAGACAGTTTGCTGCTTCACATAAATTGTTGGAGGGAGATGTTTTGGTCTTCCAATTAGTTGAACCTGCCAAATTTAAGGTTTACGTAATAAGGTCAAATGATTTTACTGAAGTTGATGGGGCTCTTGGGCTTCTAAGTTTGGATGCTCAAACAAAATATAATGATGCAGATAAAGATAATGCAGAAACAGGTGCAGTGGCTTGTAATGAGTCTAACAGGAAACGCACAAAGTCTCCTCCTGAAGTTGTTCAAAAGAAGAAAAAGATCGACTTGCCAGTTGAACACTCTGAAATCGATAGTGAGGAAGTTGATTCAGAAGTTGTGGAAAGCTCCAGGTTACCTGGGACAACTCTTCAATTTGAGGACATCAAAAGTTTTGAGAACTTCAACATTCTTGTAAATGGATTGCTTGTAGATTCTGAGTTCCCCGAAGATATACGAAGGAAGTACTACAAGCTCTGCCGCAGTCAGAATGCATTTCTTCATGAAAATGTTATGAAGGGAACATATTATAAATTGATTGTAGGAGCTATATTTGAAACTGTACATATTGTCGACGCCATTAAAGCTTGCCAGATTACCACGACTCAAGATGATTTTGTGTGTTGGGACAAATACTTGAGGGCCTTTGAGTTGTTGGGCATGAATGTTGGATTTATACGTGCGCGGTTATGCCGGCTTGCAAACCTTGCCTTTGACTCAGAAGATGCTATGGATGCGAAGACATATAAAGAAGCTAGAAAAGAACGAGCTCGAGCAGGACTAGGACTAAGAAGTATCGAGACAAAGATTCAGGGGTTGAGGGAGGCATGCCATGAATTTAGTAGTGCTATCGAGAGTCTGAGGTCCAAAGCTGAAGTCTACAATCAAAGGTTCCAGGAAGAGGTTACTGCTCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

425

Amino Acids

47.96

Weight (kDa)

6.31

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 67 - 157 1.3e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 888
Acc36I ACCTGC 1 cut(s) 448
AccI GTMKAC 2 cut(s) 930, 1236
AccII CGCG 1 cut(s) 1042
AciI CCGC 2 cut(s) 838, 1042
AcsI RAATTY 2 cut(s) 446, 1193
AcuI CTGAAG 3 cut(s) 504, 636, 1251
AcvI CACGTG 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 933
AfaI GTAC 3 cut(s) 341, 824, 921
AfiI CCNNNNNNNGG 3 cut(s) 110, 125, 1258
AflII CTTAAG 1 cut(s) 79
AgsI TTSAA 7 cut(s) 332, 437, 626, 656, 731, 763, 914
AjnI CCWGG 3 cut(s) 707, 715, 1251
AloI GAACNNNNNNTCC 2 cut(s) 174, 206
Alw21I GWGCWC 2 cut(s) 160, 1129
Alw26I GTCTC 3 cut(s) 370, 612, 1151
Ama87I CYCGRG 1 cut(s) 1128
AoxI GGCC 1 cut(s) 999
ApeKI GCWGC 2 cut(s) 47, 386
ApoI RAATTY 2 cut(s) 446, 1193
ArsI GACNNNNNNTTYG 2 cut(s) 1218, 1250
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 1 cut(s) 1042
AspS9I GGNCC 2 cut(s) 999, 1221
AsuHPI GGTGA 1 cut(s) 108
AvaI CYCGRG 1 cut(s) 1128
AvaII GGWCC 1 cut(s) 1221
BanII GRGCYC 2 cut(s) 499, 1129
BbrPI CACGTG 1 cut(s) 125
BbsI GAAGAC 3 cut(s) 328, 415, 1103
Bbv12I GWGCWC 2 cut(s) 160, 1129
BbvI GCAGC 2 cut(s) 34, 373
BccI CCATC 3 cut(s) 104, 238, 485
BciT130I CCWGG 3 cut(s) 709, 717, 1253
BcoDI GTCTC 3 cut(s) 370, 612, 1151
BfaI CTAG 5 cut(s) 179, 195, 231, 1113, 1139
BfrI CTTAAG 1 cut(s) 79
BfuAI ACCTGC 1 cut(s) 448
BisI GCNGC 3 cut(s) 48, 387, 838
BlsI GCNGC 3 cut(s) 49, 388, 839
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 3 cut(s) 709, 717, 1253
Bme18I GGWCC 1 cut(s) 1221
BmeT110I CYCGRG 1 cut(s) 1128
BmgT120I GGNCC 2 cut(s) 999, 1221
BmiI GGNNCC 1 cut(s) 1250
BmrFI CCNGG 3 cut(s) 709, 717, 1253
BmsI GCATC 4 cut(s) 508, 529, 1072, 1081
BpiI GAAGAC 3 cut(s) 328, 415, 1103
BpmI CTGGAG 1 cut(s) 691
BpuEI CTTGAG 3 cut(s) 59, 948, 1015
Bsa29I ATCGAT 1 cut(s) 669
BsaAI YACGTR 3 cut(s) 125, 460, 1037
BsaHI GRCGYC 1 cut(s) 933
BsaJI CCNNGG 2 cut(s) 716, 1271
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 2 cut(s) 592, 622
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 125, 1258
Bse118I RCCGGY 1 cut(s) 1050
Bse1I ACTGG 1 cut(s) 650
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 3 cut(s) 709, 717, 1253
BseCI ATCGAT 1 cut(s) 669
BseDI CCNNGG 2 cut(s) 716, 1271
BseGI GGATG 3 cut(s) 249, 523, 1096
BseLI CCNNNNNNNGG 3 cut(s) 110, 125, 1258
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 4 cut(s) 353, 786, 1089, 1208
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 2 cut(s) 203, 600
BseXI GCAGC 2 cut(s) 34, 373
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 1042
BshFI GGCC 1 cut(s) 1001
BshVI ATCGAT 1 cut(s) 669
BsiHKAI GWGCWC 2 cut(s) 160, 1129
BsiHKCI CYCGRG 1 cut(s) 1128
BsiSI CCGG 2 cut(s) 357, 1051
BslFI GGGAC 3 cut(s) 261, 733, 998
BslI CCNNNNNNNGG 3 cut(s) 110, 125, 1258
BsmAI GTCTC 3 cut(s) 370, 612, 1151
BsmFI GGGAC 3 cut(s) 261, 733, 998
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 1 cut(s) 1001
BsoBI CYCGRG 1 cut(s) 1128
Bsp1286I GDGCHC 3 cut(s) 160, 499, 1129
Bsp1407I TGTACA 1 cut(s) 919
Bsp143I GATC 3 cut(s) 170, 218, 639
BspACI CCGC 2 cut(s) 838, 1042
BspANI GGCC 1 cut(s) 1001
BspCNI CTCAG 4 cut(s) 354, 787, 1088, 1209
BspDI ATCGAT 1 cut(s) 669
BspFNI CGCG 1 cut(s) 1042
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 1 cut(s) 1250
BspMI ACCTGC 1 cut(s) 448
BspTI CTTAAG 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 1050
BsrGI TGTACA 1 cut(s) 919
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 1050
BssECI CCNNGG 2 cut(s) 716, 1271
BssMI GATC 3 cut(s) 170, 218, 639
BssNI GRCGYC 1 cut(s) 933
BssT1I CCWWGG 1 cut(s) 1271
Bst2UI CCWGG 3 cut(s) 709, 717, 1253
Bst4CI ACNGT 3 cut(s) 327, 381, 919
Bst6I CTCTTC 2 cut(s) 732, 1251
BstACI GRCGYC 1 cut(s) 933
BstAFI CTTAAG 1 cut(s) 79
BstAPI GCANNNNNTGC 1 cut(s) 1064
BstAUI TGTACA 1 cut(s) 919
BstBAI YACGTR 3 cut(s) 125, 460, 1037
BstC8I GCNNGC 6 cut(s) 45, 349, 946, 1052, 1056, 1186
BstDEI CTNAG 7 cut(s) 336, 362, 509, 795, 1075, 1145, 1217
BstEII GGTNACC 1 cut(s) 711
BstF5I GGATG 3 cut(s) 249, 523, 1096
BstFNI CGCG 1 cut(s) 1042
BstHHI GCGC 1 cut(s) 1042
BstKTI GATC 3 cut(s) 173, 221, 642
BstMAI GTCTC 3 cut(s) 370, 612, 1151
BstMBI GATC 3 cut(s) 170, 218, 639
BstMWI GCNNNNNNNGC 3 cut(s) 941, 1064, 1091
BstNI CCWGG 3 cut(s) 709, 717, 1253
BstNSI RCATGY 2 cut(s) 351, 1188
BstPI GGTNACC 1 cut(s) 711
BstSCI CCNGG 3 cut(s) 707, 715, 1251
BstSNI TACGTA 1 cut(s) 460
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 2 cut(s) 34, 373
BstV2I GAAGAC 3 cut(s) 328, 415, 1103
Bsu15I ATCGAT 1 cut(s) 669
BsuRI GGCC 1 cut(s) 1001
BsuTUI ATCGAT 1 cut(s) 669
BtsCI GGATG 3 cut(s) 249, 523, 1096
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 2 cut(s) 69, 576
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 6 cut(s) 45, 349, 946, 1052, 1056, 1186
CciI TCATGA 1 cut(s) 859
CfoI GCGC 1 cut(s) 1042
Cfr10I RCCGGY 1 cut(s) 1050
Cfr13I GGNCC 2 cut(s) 999, 1221
ClaI ATCGAT 1 cut(s) 669
CseI GACGC 1 cut(s) 941
Csp6I GTAC 3 cut(s) 340, 823, 920
CviAII CATG 6 cut(s) 224, 348, 860, 1018, 1185, 1190
CviQI GTAC 3 cut(s) 340, 823, 920
DdeI CTNAG 7 cut(s) 336, 362, 509, 795, 1075, 1145, 1217
DpnI GATC 3 cut(s) 172, 220, 641
DpnII GATC 3 cut(s) 170, 218, 639
Eam1104I CTCTTC 2 cut(s) 732, 1251
EarI CTCTTC 2 cut(s) 732, 1251
Ecl136II GAGCTC 1 cut(s) 1127
Eco105I TACGTA 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 1271
Eco24I GRGCYC 2 cut(s) 499, 1129
Eco47I GGWCC 1 cut(s) 1221
Eco53kI GAGCTC 1 cut(s) 1127
Eco57I CTGAAG 3 cut(s) 504, 636, 1251
Eco72I CACGTG 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 1128
Eco91I GGTNACC 1 cut(s) 711
EcoICRI GAGCTC 1 cut(s) 1127
EcoO109I RGGNCCY 1 cut(s) 999
EcoO65I GGTNACC 1 cut(s) 711
EcoRII CCWGG 3 cut(s) 707, 715, 1251
EcoT14I CCWWGG 1 cut(s) 1271
EcoT22I ATGCAT 1 cut(s) 853
EcoT38I GRGCYC 2 cut(s) 499, 1129
ErhI CCWWGG 1 cut(s) 1271
FaeI CATG 6 cut(s) 227, 351, 863, 1021, 1188, 1193
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 3 cut(s) 261, 733, 998
FatI CATG 6 cut(s) 223, 347, 859, 1017, 1184, 1189
FblI GTMKAC 2 cut(s) 930, 1236
Fnu4HI GCNGC 3 cut(s) 48, 387, 838
FokI GGATG 3 cut(s) 256, 530, 1103
FriOI GRGCYC 2 cut(s) 499, 1129
Fsp4HI GCNGC 3 cut(s) 48, 387, 838
FspBI CTAG 5 cut(s) 179, 195, 231, 1113, 1139
GlaI GCGC 1 cut(s) 1041
GluI GCNGC 3 cut(s) 48, 387, 838
GsuI CTGGAG 1 cut(s) 691
HaeIII GGCC 1 cut(s) 1001
HapII CCGG 2 cut(s) 357, 1051
HgaI GACGC 1 cut(s) 941
HhaI GCGC 1 cut(s) 1042
Hin1I GRCGYC 1 cut(s) 933
Hin1II CATG 6 cut(s) 227, 351, 863, 1021, 1188, 1193
Hin6I GCGC 1 cut(s) 1040
HinP1I GCGC 1 cut(s) 1040
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 8 cut(s) 301, 584, 686, 791, 961, 1073, 1165, 1213
HpaII CCGG 2 cut(s) 357, 1051
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 5 cut(s) 28, 72, 457, 931, 1237
Hpy188I TCNGA 9 cut(s) 105, 170, 218, 664, 691, 796, 846, 1078, 1218
Hpy188III TCNNGA 7 cut(s) 202, 305, 614, 860, 965, 1156, 1210
Hpy8I GTNNAC 5 cut(s) 28, 72, 457, 931, 1237
Hpy99I CGWCG 1 cut(s) 935
HpyAV CCTTC 4 cut(s) 264, 308, 810, 868
HpyCH4III ACNGT 3 cut(s) 327, 381, 919
HpyCH4IV ACGT 3 cut(s) 124, 459, 1036
HpyF10VI GCNNNNNNNGC 3 cut(s) 941, 1064, 1091
HpyF3I CTNAG 7 cut(s) 336, 362, 509, 795, 1075, 1145, 1217
HpySE526I ACGT 3 cut(s) 124, 459, 1036
Hsp92I GRCGYC 1 cut(s) 933
Hsp92II CATG 6 cut(s) 227, 351, 863, 1021, 1188, 1193
HspAI GCGC 1 cut(s) 1040
KroI GCCGGC 1 cut(s) 1050
KroNI GCCGGC 1 cut(s) 1052
Kzo9I GATC 3 cut(s) 170, 218, 639
LmnI GCTCC 4 cut(s) 7, 710, 902, 1273
Lsp1109I GCAGC 2 cut(s) 34, 373
LweI GCATC 4 cut(s) 508, 529, 1072, 1081
MaeI CTAG 5 cut(s) 179, 195, 231, 1113, 1139
MaeII ACGT 3 cut(s) 124, 459, 1036
MaeIII GTNAC 2 cut(s) 711, 1261
MalI GATC 3 cut(s) 172, 220, 641
MboI GATC 3 cut(s) 170, 218, 639
MhlI GDGCHC 3 cut(s) 160, 499, 1129
MluCI AATT 6 cut(s) 398, 428, 446, 731, 890, 1193
MlyI GAGTC 5 cut(s) 295, 593, 955, 1067, 1222
MmeI TCCRAC 3 cut(s) 353, 384, 1006
Mph1103I ATGCAT 1 cut(s) 853
MroNI GCCGGC 1 cut(s) 1050
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 3 cut(s) 80, 450, 939
MslI CAYNNNNRTG 1 cut(s) 864
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 2 cut(s) 357, 1051
MspR9I CCNGG 3 cut(s) 709, 717, 1253
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 3 cut(s) 709, 717, 1253
MvnI CGCG 1 cut(s) 1042
MwoI GCNNNNNNNGC 3 cut(s) 941, 1064, 1091
NaeI GCCGGC 1 cut(s) 1052
NdeII GATC 3 cut(s) 170, 218, 639
NgoMIV GCCGGC 1 cut(s) 1050
NlaIII CATG 6 cut(s) 227, 351, 863, 1021, 1188, 1193
NlaIV GGNNCC 1 cut(s) 1250
NsiI ATGCAT 1 cut(s) 853
NspI RCATGY 2 cut(s) 351, 1188
PaeI GCATGC 2 cut(s) 351, 1188
PaeR7I CTCGAG 1 cut(s) 1128
PagI TCATGA 1 cut(s) 859
PctI GAATGC 1 cut(s) 853
PdiI GCCGGC 1 cut(s) 1052
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 3 cut(s) 686, 791, 1165
PfoI TCCNGGA 1 cut(s) 1251
PkrI GCNGC 3 cut(s) 49, 388, 839
PleI GAGTC 5 cut(s) 295, 592, 955, 1067, 1221
PmaCI CACGTG 1 cut(s) 125
PmlI CACGTG 1 cut(s) 125
PpsI GAGTC 5 cut(s) 295, 592, 955, 1067, 1221
Ppu21I YACGTR 3 cut(s) 125, 460, 1037
PsiI TTATAA 1 cut(s) 888
Psp124BI GAGCTC 1 cut(s) 1129
Psp6I CCWGG 3 cut(s) 707, 715, 1251
PspCI CACGTG 1 cut(s) 125
PspEI GGTNACC 1 cut(s) 711
PspGI CCWGG 3 cut(s) 707, 715, 1251
PspN4I GGNNCC 1 cut(s) 1250
PspPI GGNCC 2 cut(s) 999, 1221
PspXI VCTCGAGB 1 cut(s) 1128
RsaI GTAC 3 cut(s) 341, 824, 921
RsaNI GTAC 3 cut(s) 340, 823, 920
RseI CAYNNNNRTG 1 cut(s) 864
SacI GAGCTC 1 cut(s) 1129
SalI GTCGAC 1 cut(s) 929
SaqAI TTAA 3 cut(s) 80, 450, 939
SatI GCNGC 3 cut(s) 48, 387, 838
Sau3AI GATC 3 cut(s) 170, 218, 639
Sau96I GGNCC 2 cut(s) 999, 1221
ScaI AGTACT 1 cut(s) 824
SchI GAGTC 5 cut(s) 295, 593, 955, 1067, 1222
ScrFI CCNGG 3 cut(s) 709, 717, 1253
SduI GDGCHC 3 cut(s) 160, 499, 1129
SfaNI GCATC 4 cut(s) 508, 529, 1072, 1081
Sfr274I CTCGAG 1 cut(s) 1128
SinI GGWCC 1 cut(s) 1221
SlaI CTCGAG 1 cut(s) 1128
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 5 cut(s) 38, 79, 963, 994, 1128
SmoI CTYRAG 5 cut(s) 38, 79, 963, 994, 1128
SnaBI TACGTA 1 cut(s) 460
SphI GCATGC 2 cut(s) 351, 1188
Sse9I AATT 6 cut(s) 398, 428, 446, 731, 890, 1193
SsiI CCGC 2 cut(s) 838, 1042
SspMI CTAG 5 cut(s) 179, 195, 231, 1113, 1139
SstI GAGCTC 1 cut(s) 1129
StyD4I CCNGG 3 cut(s) 707, 715, 1251
StyI CCWWGG 1 cut(s) 1271
TaaI ACNGT 3 cut(s) 327, 381, 919
TaiI ACGT 3 cut(s) 127, 462, 1039
TaqI TCGA 7 cut(s) 154, 642, 669, 930, 1129, 1155, 1209
TasI AATT 6 cut(s) 398, 428, 446, 731, 890, 1193
TatI WGTACW 3 cut(s) 339, 822, 919
TauI GCSGC 1 cut(s) 840
TfiI GAWTC 3 cut(s) 686, 791, 1165
Tru1I TTAA 3 cut(s) 80, 450, 939
Tru9I TTAA 3 cut(s) 80, 450, 939
TscAI CASTG 2 cut(s) 69, 576
TseI GCWGC 2 cut(s) 47, 386
TspDTI ATGAA 7 cut(s) 17, 327, 848, 876, 887, 1034, 1206
TspRI CASTG 2 cut(s) 69, 576
Vha464I CTTAAG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 1221
XapI RAATTY 2 cut(s) 446, 1193
XceI RCATGY 2 cut(s) 351, 1188
XhoI CTCGAG 1 cut(s) 1128
XmiI GTMKAC 2 cut(s) 930, 1236
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 5 cut(s) 179, 195, 231, 1113, 1139
ZrmI AGTACT 1 cut(s) 824
Zsp2I ATGCAT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.