pycom02g13710

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012565
Physical Location & Seq
Forward (+)
10319685 .. 10321642
1958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13710.1

Sequence Viewer

Length: 717 bp
ATGGCTCGGAAGGTTGACGGGAAGCCCCAAACGCCGCCGTCCAAACCCAGGCTCCGCCCCAAGCCCGCCGCCAACCCTAAACCTTCTTCTCTCCCCACGGGTAAGGTGAAGAAGCCAGGCAAGCCGAACGAGAAGGAAGTGGAGCATTCCATGCCTATGAAGGAGCTTTCCAATACCCAAATTACAAAGATGTTGAGTCACGCAGGGAAGTCCCCGGCCATTAGCAATACACAGTATGAGTTTCCAGTTGATGGATCACCCTCACGAGGTCAAGCAAAGTCATCTGCTGTCATTCGAGCACAGGAAGTTCGATCAAAACTAGAACGTCAATTCCCTAGCTTCGTGAAATCACTGGTCAGATCACACGTGGCCAGTTGTTTTTGGATGGGGCTTCCTTCGCTGTTCTGCAAATCACACTTACCAGATAAAGATATTACAATGACTTTGGAAGATGAAAGTGGTAGACAGTATCAGTTGAAATACATTGCATGCAAGACCGGACTGAGTGCTGGTTGGAGACAGTTTGCTGCTTCTCACAATTTACTCGAGGGAGATGTTCTGGTTTTCCAATTAGTCGAACCTACAAAATTTAAGGTTTACATAATAAGGTCAAATGGTATTGCTGAAGTTGATGGGGCTCTTTGCCTTCTAAATTTGGATAATAATACAAAACAAAAGGATTCAGGCAAGTTCATTAATCTCTCTGTTTGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

239

Amino Acids

26.34

Weight (kDa)

9.71

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 114 - 204 7.2e-19 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 251
AccI GTMKAC 1 cut(s) 463
AciI CCGC 4 cut(s) 35, 55, 66, 69
AclWI GGATC 1 cut(s) 262
AcoI YGGCCR 2 cut(s) 216, 369
AcsI RAATTY 2 cut(s) 587, 652
AcuI CTGAAG 1 cut(s) 645
AcvI CACGTG 1 cut(s) 367
AfiI CCNNNNNNNGG 3 cut(s) 48, 251, 266
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 1 cut(s) 478
AjnI CCWGG 2 cut(s) 47, 115
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 2 cut(s) 166, 339
AluI AGCT 2 cut(s) 166, 339
Alw21I GWGCWC 1 cut(s) 301
Alw26I GTCTC 1 cut(s) 511
AlwI GGATC 1 cut(s) 262
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 2 cut(s) 216, 369
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 2 cut(s) 587, 652
AseI ATTAAT 1 cut(s) 696
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 118, 249
AvaI CYCGRG 1 cut(s) 545
BalI TGGCCA 1 cut(s) 371
BanII GRGCYC 1 cut(s) 640
BauI CACGAG 1 cut(s) 264
BbrPI CACGTG 1 cut(s) 367
Bbv12I GWGCWC 1 cut(s) 301
BbvI GCAGC 1 cut(s) 514
BccI CCATC 3 cut(s) 245, 379, 626
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 2 cut(s) 49, 117
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 511
BfaI CTAG 2 cut(s) 320, 336
BisI GCNGC 3 cut(s) 35, 69, 528
BlsI GCNGC 3 cut(s) 36, 70, 529
Bme1390I CCNGG 3 cut(s) 49, 117, 215
BmeT110I CYCGRG 1 cut(s) 545
BmiI GGNNCC 1 cut(s) 53
BmrFI CCNGG 3 cut(s) 49, 117, 215
BpuMI CCSGG 1 cut(s) 215
BsaAI YACGTR 1 cut(s) 367
BsaJI CCNNGG 3 cut(s) 47, 96, 213
BsaWI WCCGGW 1 cut(s) 497
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 251, 266
Bse1I ACTGG 3 cut(s) 245, 357, 372
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 2 cut(s) 49, 117
BseDI CCNNGG 3 cut(s) 47, 96, 213
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 3 cut(s) 48, 251, 266
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 3 cut(s) 245, 357, 372
BseXI GCAGC 1 cut(s) 514
BshFI GGCC 2 cut(s) 218, 371
BsiHKAI GWGCWC 1 cut(s) 301
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 2 cut(s) 215, 498
BslFI GGGAC 1 cut(s) 196
BslI CCNNNNNNNGG 3 cut(s) 48, 251, 266
BsmAI GTCTC 1 cut(s) 511
BsmFI GGGAC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 2 cut(s) 218, 371
BsoBI CYCGRG 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 301, 640
Bsp143I GATC 3 cut(s) 254, 311, 359
BspACI CCGC 4 cut(s) 35, 55, 66, 69
BspANI GGCC 2 cut(s) 218, 371
BspCNI CTCAG 1 cut(s) 495
BspLI GGNNCC 1 cut(s) 53
BspPI GGATC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 483
BsrI ACTGG 3 cut(s) 245, 357, 372
BssECI CCNNGG 3 cut(s) 47, 96, 213
BssMI GATC 3 cut(s) 254, 311, 359
BssSI CACGAG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 2 cut(s) 49, 117
Bst4CI ACNGT 3 cut(s) 234, 468, 522
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 1 cut(s) 367
BstC8I GCNNGC 3 cut(s) 66, 122, 490
BstDEI CTNAG 1 cut(s) 503
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 390
BstKTI GATC 3 cut(s) 257, 314, 362
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 3 cut(s) 254, 311, 359
BstMWI GCNNNNNNNGC 4 cut(s) 31, 121, 151, 397
BstNI CCWGG 2 cut(s) 49, 117
BstNSI RCATGY 1 cut(s) 492
BstSCI CCNGG 3 cut(s) 47, 115, 213
BstV1I GCAGC 1 cut(s) 514
BsuRI GGCC 2 cut(s) 218, 371
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 390
BtsIMutI CAGTG 1 cut(s) 350
Cac8I GCNNGC 3 cut(s) 66, 122, 490
CviAII CATG 2 cut(s) 151, 489
DdeI CTNAG 1 cut(s) 503
DpnI GATC 3 cut(s) 256, 313, 361
DpnII GATC 3 cut(s) 254, 311, 359
EaeI YGGCCR 2 cut(s) 216, 369
EciI GGCGGA 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 645
Eco72I CACGTG 1 cut(s) 367
Eco88I CYCGRG 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 47, 115
EcoT38I GRGCYC 1 cut(s) 640
FaeI CATG 2 cut(s) 154, 492
FaiI YATR 5 cut(s) 152, 158, 237, 490, 602
FaqI GGGAC 1 cut(s) 196
FatI CATG 2 cut(s) 150, 488
FauI CCCGC 1 cut(s) 73
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 3 cut(s) 35, 69, 528
FokI GGATG 1 cut(s) 397
FriOI GRGCYC 1 cut(s) 640
Fsp4HI GCNGC 3 cut(s) 35, 69, 528
FspBI CTAG 2 cut(s) 320, 336
GluI GCNGC 3 cut(s) 35, 69, 528
HaeIII GGCC 2 cut(s) 218, 371
HapII CCGG 2 cut(s) 215, 498
Hin1II CATG 2 cut(s) 154, 492
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 2 cut(s) 196, 680
HpaII CCGG 2 cut(s) 215, 498
HphI GGTGA 2 cut(s) 118, 249
Hpy166II GTNNAC 3 cut(s) 16, 464, 598
Hpy188I TCNGA 3 cut(s) 9, 359, 716
Hpy188III TCNNGA 2 cut(s) 264, 343
Hpy8I GTNNAC 3 cut(s) 16, 464, 598
HpyAV CCTTC 6 cut(s) 4, 93, 127, 154, 405, 656
HpyCH4III ACNGT 3 cut(s) 234, 468, 522
HpyCH4IV ACGT 2 cut(s) 325, 366
HpyCH4V TGCA 3 cut(s) 408, 488, 492
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 121, 151, 397
HpyF3I CTNAG 1 cut(s) 503
HpySE526I ACGT 2 cut(s) 325, 366
Hsp92II CATG 2 cut(s) 154, 492
Kzo9I GATC 3 cut(s) 254, 311, 359
LmnI GCTCC 3 cut(s) 57, 142, 163
Lsp1109I GCAGC 1 cut(s) 514
MaeI CTAG 2 cut(s) 320, 336
MaeII ACGT 2 cut(s) 325, 366
MaeIII GTNAC 1 cut(s) 197
MalI GATC 3 cut(s) 256, 313, 361
MboI GATC 3 cut(s) 254, 311, 359
MboII GAAGA 3 cut(s) 78, 121, 461
MhlI GDGCHC 2 cut(s) 301, 640
MlsI TGGCCA 1 cut(s) 371
MluCI AATT 6 cut(s) 180, 329, 538, 569, 587, 652
MluNI TGGCCA 1 cut(s) 371
MlyI GAGTC 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 3 cut(s) 260, 271, 541
Mox20I TGGCCA 1 cut(s) 371
MscI TGGCCA 1 cut(s) 371
MseI TTAA 2 cut(s) 591, 696
MslI CAYNNNNRTG 1 cut(s) 155
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 2 cut(s) 215, 498
MspR9I CCNGG 3 cut(s) 49, 117, 215
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 2 cut(s) 49, 117
MwoI GCNNNNNNNGC 4 cut(s) 31, 121, 151, 397
NciI CCSGG 1 cut(s) 215
NdeII GATC 3 cut(s) 254, 311, 359
NlaIII CATG 2 cut(s) 154, 492
NlaIV GGNNCC 1 cut(s) 53
NmuCI GTSAC 1 cut(s) 197
NspI RCATGY 1 cut(s) 492
PaeI GCATGC 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 545
PctI GAATGC 1 cut(s) 145
PfeI GAWTC 1 cut(s) 680
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 3 cut(s) 36, 70, 529
PleI GAGTC 1 cut(s) 204
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 1 cut(s) 204
Ppu21I YACGTR 1 cut(s) 367
PshBI ATTAAT 1 cut(s) 696
Psp6I CCWGG 2 cut(s) 47, 115
PspCI CACGTG 1 cut(s) 367
PspGI CCWGG 2 cut(s) 47, 115
PspN4I GGNNCC 1 cut(s) 53
PspXI VCTCGAGB 1 cut(s) 545
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 2 cut(s) 591, 696
SatI GCNGC 3 cut(s) 35, 69, 528
Sau3AI GATC 3 cut(s) 254, 311, 359
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 3 cut(s) 49, 117, 215
SduI GDGCHC 2 cut(s) 301, 640
Sfr274I CTCGAG 1 cut(s) 545
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
SphI GCATGC 1 cut(s) 492
Sse9I AATT 6 cut(s) 180, 329, 538, 569, 587, 652
SsiI CCGC 4 cut(s) 35, 55, 66, 69
SspMI CTAG 2 cut(s) 320, 336
StyD4I CCNGG 3 cut(s) 47, 115, 213
TaaI ACNGT 3 cut(s) 234, 468, 522
TaiI ACGT 2 cut(s) 328, 369
TaqI TCGA 4 cut(s) 295, 310, 546, 576
TasI AATT 6 cut(s) 180, 329, 538, 569, 587, 652
TauI GCSGC 2 cut(s) 37, 71
TfiI GAWTC 1 cut(s) 680
Tru1I TTAA 2 cut(s) 591, 696
Tru9I TTAA 2 cut(s) 591, 696
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 3 cut(s) 173, 468, 682
TspRI CASTG 1 cut(s) 357
Van91I CCANNNNNTGG 1 cut(s) 251
VspI ATTAAT 1 cut(s) 696
XapI RAATTY 2 cut(s) 587, 652
XceI RCATGY 1 cut(s) 492
XhoI CTCGAG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 463
XspI CTAG 2 cut(s) 320, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.