MD15G1282300.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012565
Physical Location & Seq
Forward (+)
25492021 .. 25495177
3157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1282300.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGGCTCGGAATGTTAAGAACGCCAAGCCCCAAACGCCGCTGTCCAAGGAGGACGGCCACCTGACTCAGCCTCCATTGCCCAAACCCAGGCTCCGCCCCAAGCCCGCCACCAACCCTAAACCTTCTTCTCTCCCCACGGGGAAGTTGAAGAAGCCAGGGAAGCCAAAAGAGCAAGAAGTGGAGTATTCCATGCCTATGAAGGAGCTTTCTAATACCCAAATTACAAAGATGTTGAGTCATGCAGGGCAGTCCCCAGCCATTAGCAATGCGCAGTATGATTTTCCAGTTGATGGATCACCCTCACGAGGTCAAGCAAAGTCATCTGCTGTCATTCGAGCGCAGGAAGTTCGATCAAAACTAGAACCTCAATTCCCCAGCTTTGTGAAATCACTGGTCAGATCACACGTGGCTAGTTGTTTTTGGATGGGGCTTCCTTCGCTGTTCTGCAAATCACACTTACCAGATAAAGATATTACAATGACTTTGGAAGATGAAAGTGGTAGACAGTATCAGTCGAAATACATTGCATGCAAGACCGGACTAAGTTCTGGTTGGAGGCAGTTTGCTGCTTCGCACAATTTGCTCGAGGGAGATGTTCTGGCCTTCCAATTAGTTGAACCTACAAAATTTAAGGTTTACATAATAAGGTCAAATGGTTTGGCTGAAGTTGATGGGGATCTTGGCCTTCCAAATTTGGATAATAATACAAAACAAAAGGATGCAGATAAAGATAATGCAGAAACGAGCACTACGCCTTGTAATGACTCTACCAAGAAACGTGCAGAAACAAGCACTACGCCTTGGAATGACTCTAATAGGAAACGCGCGCATTCTCCTCAATTAGTAGCTCCAAAGAAGAAAAAGATTGGTTTGCAGCGAGCCAAACAATCTGAAACCGATAGTGAGGAGGTTGGTTCTGAAGTTTCAGAAGATCTCAAGTCACCTGTGGCAACTCTTCAATTTGAGGACATCCGAAGTTATGAGAATTTCAACATTCTAGTAGATGGGTTGTTTCTAGATCCTGAGTTCCCCGAAGATGTACGAAGAAAGTACTACAAGCTCTGTCGCAGCCAGAAAGCATTTCTTCATGAAAATGTTATCAAGGGCATGAATCATAATATGATTATAGGAGCTATATCTGAAACCGTAAATATTGCTGATTCCATTGAAGCTTGCAAGGTCACTGCGCTTCGAGATGATTTTGTGTCTTGGGACAAGTATTTAACGGCCTTTGAGTTGTTGGGCATGAATGTTGGATTTTTACGTGAACGGGTATGTCGGCTTGCAAACCTTGCCGACAACTCGGAAGCTGCCGTGGAGGCAAAGATGTTTAAAGAAGCTACAATTGAGCGATCTCAGGCAGGAATAGGATTAAGAAATATCGAGATGAAGCTGGAGGAACTGATGGAGGCGTGCGATGAATTTGGTGCTGCCATTGAGAGCCTGAGATCGAAAGCTGAAATCAACAAGCTCAGGTTCCAGGAAGAGGTTACTGCTCCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

502

Amino Acids

56.0

Weight (kDa)

8.24

Isoelectric Point (pI)

58.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 127 - 217 9.8e-18 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 270
AccB7I CCANNNNNTGG 1 cut(s) 290
AccI GTMKAC 1 cut(s) 502
AccII CGCG 2 cut(s) 825, 827
AciI CCGC 3 cut(s) 38, 94, 105
AclWI GGATC 3 cut(s) 301, 684, 1013
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 4 cut(s) 626, 691, 985, 1421
AcuI CTGAAG 2 cut(s) 684, 939
AcvI CACGTG 1 cut(s) 406
AfaI GTAC 2 cut(s) 1041, 1052
AfiI CCNNNNNNNGG 4 cut(s) 87, 290, 305, 1486
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 5 cut(s) 148, 617, 959, 991, 1169
AjnI CCWGG 3 cut(s) 86, 154, 1479
AloI GAACNNNNNNTCC 2 cut(s) 354, 386
Alw21I GWGCWC 1 cut(s) 749
AlwI GGATC 3 cut(s) 301, 684, 1013
Ama87I CYCGRG 1 cut(s) 584
AoxI GGCC 4 cut(s) 55, 600, 682, 1227
ApeKI GCWGC 5 cut(s) 566, 874, 1068, 1310, 1430
ApoI RAATTY 4 cut(s) 626, 691, 985, 1421
AspLEI GCGC 5 cut(s) 271, 340, 827, 829, 1189
AsuHPI GGTGA 2 cut(s) 288, 933
AvaI CYCGRG 1 cut(s) 584
BauI CACGAG 1 cut(s) 303
BbrPI CACGTG 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 749
BbvI GCAGC 5 cut(s) 553, 886, 1080, 1297, 1417
BccI CCATC 5 cut(s) 284, 418, 665, 998, 1399
BceAI ACGGC 3 cut(s) 70, 1242, 1298
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 3 cut(s) 88, 156, 1481
BfaI CTAG 4 cut(s) 359, 411, 998, 1016
BglI GCCNNNNNGGC 1 cut(s) 1319
BglII AGATCT 1 cut(s) 931
BisI GCNGC 6 cut(s) 38, 567, 875, 1069, 1311, 1431
BlsI GCNGC 6 cut(s) 39, 568, 876, 1070, 1312, 1432
BmcAI AGTACT 1 cut(s) 1052
Bme1390I CCNGG 3 cut(s) 88, 156, 1481
BmeT110I CYCGRG 1 cut(s) 584
BmiI GGNNCC 2 cut(s) 92, 1478
BmrFI CCNGG 3 cut(s) 88, 156, 1481
BmsI GCATC 1 cut(s) 709
BpmI CTGGAG 1 cut(s) 1415
Bpu10I CCTNAGC 1 cut(s) 1472
BpuEI CTTGAG 1 cut(s) 920
BsaAI YACGTR 2 cut(s) 406, 1265
BsaBI GATNNNNATC 1 cut(s) 675
BsaJI CCNNGG 7 cut(s) 45, 86, 135, 155, 800, 1314, 1499
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 4 cut(s) 55, 75, 85, 105
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 290, 305, 1486
Bse1I ACTGG 2 cut(s) 284, 396
Bse3DI GCAATG 3 cut(s) 74, 271, 522
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 3 cut(s) 88, 156, 1481
BseDI CCNNGG 7 cut(s) 45, 86, 135, 155, 800, 1314, 1499
BseGI GGATG 3 cut(s) 429, 724, 969
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 4 cut(s) 87, 290, 305, 1486
BseMI GCAATG 3 cut(s) 74, 271, 522
BseMII CTCAG 5 cut(s) 80, 1014, 1370, 1436, 1486
BseNI ACTGG 2 cut(s) 284, 396
BsePI GCGCGC 1 cut(s) 825
BseRI GAGGAG 2 cut(s) 825, 920
BseXI GCAGC 5 cut(s) 553, 886, 1080, 1297, 1417
BseYI CCCAGC 2 cut(s) 253, 374
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 2 cut(s) 825, 827
BshFI GGCC 4 cut(s) 57, 602, 684, 1229
BsiHKAI GWGCWC 1 cut(s) 749
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 1 cut(s) 537
BslFI GGGAC 2 cut(s) 235, 1226
BslI CCNNNNNNNGG 4 cut(s) 87, 290, 305, 1486
BsmFI GGGAC 2 cut(s) 235, 1226
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 4 cut(s) 57, 602, 684, 1229
BsoBI CYCGRG 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 749
Bsp143I GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
BspACI CCGC 3 cut(s) 38, 94, 105
BspANI GGCC 4 cut(s) 57, 602, 684, 1229
BspCNI CTCAG 5 cut(s) 79, 1015, 1369, 1437, 1485
BspFNI CGCG 2 cut(s) 825, 827
BspHI TCATGA 1 cut(s) 1087
BspLI GGNNCC 2 cut(s) 92, 1478
BspPI GGATC 3 cut(s) 301, 684, 1013
BsrDI GCAATG 3 cut(s) 74, 271, 522
BsrI ACTGG 2 cut(s) 284, 396
BssECI CCNNGG 7 cut(s) 45, 86, 135, 155, 800, 1314, 1499
BssHII GCGCGC 1 cut(s) 825
BssMI GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
BssSI CACGAG 1 cut(s) 303
BssT1I CCWWGG 3 cut(s) 45, 800, 1499
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 3 cut(s) 88, 156, 1481
Bst4CI ACNGT 2 cut(s) 507, 1147
Bst6I CTCTTC 2 cut(s) 960, 1479
BstAPI GCANNNNNTGC 2 cut(s) 580, 1292
BstBAI YACGTR 2 cut(s) 406, 1265
BstC8I GCNNGC 7 cut(s) 105, 529, 827, 879, 1174, 1284, 1414
BstDEI CTNAG 6 cut(s) 66, 542, 1023, 1356, 1445, 1472
BstDSI CCRYGG 2 cut(s) 135, 1314
BstF5I GGATG 3 cut(s) 429, 724, 969
BstFNI CGCG 2 cut(s) 825, 827
BstHHI GCGC 5 cut(s) 271, 340, 827, 829, 1189
BstKTI GATC 8 cut(s) 296, 353, 401, 679, 934, 1021, 1355, 1451
BstMBI GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
BstMWI GCNNNNNNNGC 8 cut(s) 34, 76, 160, 169, 436, 580, 1292, 1319
BstNI CCWGG 3 cut(s) 88, 156, 1481
BstNSI RCATGY 1 cut(s) 531
BstSCI CCNGG 3 cut(s) 86, 154, 1479
BstUI CGCG 2 cut(s) 825, 827
BstV1I GCAGC 5 cut(s) 553, 886, 1080, 1297, 1417
BstX2I RGATCY 3 cut(s) 676, 931, 1018
BstYI RGATCY 3 cut(s) 676, 931, 1018
BsuRI GGCC 4 cut(s) 57, 602, 684, 1229
BtgI CCRYGG 2 cut(s) 135, 1314
BtgZI GCGATG 1 cut(s) 1431
BtsCI GGATG 3 cut(s) 429, 724, 969
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 2 cut(s) 389, 1182
Cac8I GCNNGC 7 cut(s) 105, 529, 827, 879, 1174, 1284, 1414
CciI TCATGA 1 cut(s) 1087
CfoI GCGC 5 cut(s) 271, 340, 827, 829, 1189
Csp6I GTAC 2 cut(s) 1040, 1051
CviAII CATG 6 cut(s) 190, 239, 528, 1088, 1108, 1246
CviQI GTAC 2 cut(s) 1040, 1051
DdeI CTNAG 6 cut(s) 66, 542, 1023, 1356, 1445, 1472
DpnI GATC 8 cut(s) 295, 352, 400, 678, 933, 1020, 1354, 1450
DpnII GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
DraI TTTAAA 1 cut(s) 1333
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 960, 1479
EarI CTCTTC 2 cut(s) 960, 1479
EciI GGCGGA 1 cut(s) 83
Eco130I CCWWGG 3 cut(s) 45, 800, 1499
Eco57I CTGAAG 2 cut(s) 684, 939
Eco72I CACGTG 1 cut(s) 406
Eco88I CYCGRG 1 cut(s) 584
EcoRII CCWGG 3 cut(s) 86, 154, 1479
EcoT14I CCWWGG 3 cut(s) 45, 800, 1499
ErhI CCWWGG 3 cut(s) 45, 800, 1499
FaeI CATG 6 cut(s) 193, 242, 531, 1091, 1111, 1249
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 2 cut(s) 235, 1226
FatI CATG 6 cut(s) 189, 238, 527, 1087, 1107, 1245
FauI CCCGC 1 cut(s) 112
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 6 cut(s) 38, 567, 875, 1069, 1311, 1431
FokI GGATG 3 cut(s) 436, 731, 956
Fsp4HI GCNGC 6 cut(s) 38, 567, 875, 1069, 1311, 1431
FspBI CTAG 4 cut(s) 359, 411, 998, 1016
FspI TGCGCA 1 cut(s) 270
GlaI GCGC 5 cut(s) 270, 339, 826, 828, 1188
GluI GCNGC 6 cut(s) 38, 567, 875, 1069, 1311, 1431
GsaI CCCAGC 2 cut(s) 257, 378
GsuI CTGGAG 1 cut(s) 1415
HaeIII GGCC 4 cut(s) 57, 602, 684, 1229
HapII CCGG 1 cut(s) 537
HhaI GCGC 5 cut(s) 271, 340, 827, 829, 1189
Hin1II CATG 6 cut(s) 193, 242, 531, 1091, 1111, 1249
Hin6I GCGC 5 cut(s) 269, 338, 825, 827, 1187
HinP1I GCGC 5 cut(s) 269, 338, 825, 827, 1187
HindIII AAGCTT 1 cut(s) 1170
HinfI GANTC 6 cut(s) 64, 235, 764, 809, 1111, 1160
HpaII CCGG 1 cut(s) 537
HphI GGTGA 2 cut(s) 288, 933
Hpy166II GTNNAC 3 cut(s) 503, 637, 1268
Hpy188I TCNGA 8 cut(s) 9, 398, 892, 919, 928, 974, 1141, 1306
Hpy188III TCNNGA 6 cut(s) 303, 1016, 1022, 1088, 1193, 1384
Hpy8I GTNNAC 3 cut(s) 503, 637, 1268
HpyAV CCTTC 5 cut(s) 132, 193, 444, 613, 695
HpyCH4III ACNGT 2 cut(s) 507, 1147
HpyCH4IV ACGT 3 cut(s) 405, 778, 1264
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 76, 160, 169, 436, 580, 1292, 1319
HpyF3I CTNAG 6 cut(s) 66, 542, 1023, 1356, 1445, 1472
HpySE526I ACGT 3 cut(s) 405, 778, 1264
Hsp92II CATG 6 cut(s) 193, 242, 531, 1091, 1111, 1249
HspAI GCGC 5 cut(s) 269, 338, 825, 827, 1187
Kzo9I GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
LmnI GCTCC 5 cut(s) 96, 202, 853, 1130, 1501
Lsp1109I GCAGC 5 cut(s) 553, 886, 1080, 1297, 1417
LweI GCATC 1 cut(s) 709
MaeI CTAG 4 cut(s) 359, 411, 998, 1016
MaeII ACGT 3 cut(s) 405, 778, 1264
MaeIII GTNAC 3 cut(s) 939, 1180, 1489
MalI GATC 8 cut(s) 295, 352, 400, 678, 933, 1020, 1354, 1450
MboI GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
MfeI CAATTG 1 cut(s) 1344
MflI RGATCY 3 cut(s) 676, 931, 1018
MhlI GDGCHC 1 cut(s) 749
MlyI GAGTC 4 cut(s) 58, 244, 758, 803
MmeI TCCRAC 2 cut(s) 533, 1234
MseI TTAA 5 cut(s) 15, 630, 1223, 1332, 1373
MslI CAYNNNNRTG 2 cut(s) 194, 1092
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 1 cut(s) 537
MspR9I CCNGG 3 cut(s) 88, 156, 1481
MunI CAATTG 1 cut(s) 1344
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 3 cut(s) 88, 156, 1481
MvnI CGCG 2 cut(s) 825, 827
MwoI GCNNNNNNNGC 8 cut(s) 34, 76, 160, 169, 436, 580, 1292, 1319
NdeII GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
NlaIII CATG 6 cut(s) 193, 242, 531, 1091, 1111, 1249
NlaIV GGNNCC 2 cut(s) 92, 1478
NmuCI GTSAC 2 cut(s) 939, 1180
NsbI TGCGCA 1 cut(s) 270
NspI RCATGY 1 cut(s) 531
PaeI GCATGC 1 cut(s) 531
PaeR7I CTCGAG 1 cut(s) 584
PagI TCATGA 1 cut(s) 1087
PauI GCGCGC 1 cut(s) 825
PctI GAATGC 1 cut(s) 829
PfeI GAWTC 2 cut(s) 1111, 1160
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 1479
PkrI GCNGC 6 cut(s) 39, 568, 876, 1070, 1312, 1432
PleI GAGTC 4 cut(s) 58, 243, 758, 803
PmaCI CACGTG 1 cut(s) 406
PmlI CACGTG 1 cut(s) 406
PpsI GAGTC 4 cut(s) 58, 243, 758, 803
Ppu21I YACGTR 2 cut(s) 406, 1265
Psp6I CCWGG 3 cut(s) 86, 154, 1479
PspCI CACGTG 1 cut(s) 406
PspFI CCCAGC 2 cut(s) 253, 374
PspGI CCWGG 3 cut(s) 86, 154, 1479
PspN4I GGNNCC 2 cut(s) 92, 1478
PspXI VCTCGAGB 1 cut(s) 584
PsuI RGATCY 3 cut(s) 676, 931, 1018
PteI GCGCGC 1 cut(s) 825
RsaI GTAC 2 cut(s) 1041, 1052
RsaNI GTAC 2 cut(s) 1040, 1051
RseI CAYNNNNRTG 2 cut(s) 194, 1092
SaqAI TTAA 5 cut(s) 15, 630, 1223, 1332, 1373
SatI GCNGC 6 cut(s) 38, 567, 875, 1069, 1311, 1431
Sau3AI GATC 8 cut(s) 293, 350, 398, 676, 931, 1018, 1352, 1448
ScaI AGTACT 1 cut(s) 1052
SchI GAGTC 4 cut(s) 58, 244, 758, 803
ScrFI CCNGG 3 cut(s) 88, 156, 1481
SduI GDGCHC 1 cut(s) 749
SfaNI GCATC 1 cut(s) 709
Sfr274I CTCGAG 1 cut(s) 584
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 2 cut(s) 194, 1092
SmlI CTYRAG 2 cut(s) 584, 935
SmoI CTYRAG 2 cut(s) 584, 935
SphI GCATGC 1 cut(s) 531
SsiI CCGC 3 cut(s) 38, 94, 105
SspI AATATT 1 cut(s) 1153
SspMI CTAG 4 cut(s) 359, 411, 998, 1016
StyD4I CCNGG 3 cut(s) 86, 154, 1479
StyI CCWWGG 3 cut(s) 45, 800, 1499
TaaI ACNGT 2 cut(s) 507, 1147
TaiI ACGT 3 cut(s) 408, 781, 1267
TaqI TCGA 7 cut(s) 334, 349, 515, 585, 1192, 1383, 1451
TatI WGTACW 1 cut(s) 1050
TauI GCSGC 1 cut(s) 40
TfiI GAWTC 2 cut(s) 1111, 1160
Tru1I TTAA 5 cut(s) 15, 630, 1223, 1332, 1373
Tru9I TTAA 5 cut(s) 15, 630, 1223, 1332, 1373
TscAI CASTG 2 cut(s) 396, 1189
TseFI GTSAC 2 cut(s) 939, 1180
TseI GCWGC 5 cut(s) 566, 874, 1068, 1310, 1430
Tsp45I GTSAC 2 cut(s) 939, 1180
TspDTI ATGAA 8 cut(s) 212, 507, 1076, 1104, 1124, 1262, 1403, 1434
TspRI CASTG 2 cut(s) 396, 1189
Van91I CCANNNNNTGG 1 cut(s) 290
XapI RAATTY 4 cut(s) 626, 691, 985, 1421
XbaI TCTAGA 1 cut(s) 1015
XceI RCATGY 1 cut(s) 531
XhoI CTCGAG 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 4 cut(s) 359, 411, 998, 1016
ZrmI AGTACT 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.