MD02G1172000.v1.1

CLK4-associating serine arginine rich

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004786
Physical Location & Seq
Forward (+)
14954675 .. 14956830
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1172000.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGTGGCACGAAGCTAGGAGATCGGAGCGGAAGGTCCACGACATGATGGACGCCGCTCGAAAGCGAGCTCAACGACGTGCCGTTTTCCTGGCCAAGCGCCGCGGCGACCCCCAGCAGTCGATCCAGGTCGTCGGCTCCCGTTGCCGCTTGTACCGCGATGACGGCCTCTACCAAGCCGCCGAGGACCAGCAAGGCCTGATTCCTTGGAATGGGAAGCAGGATGTTCTGATCGATAGATTTGATGGCCGAGCTCTGCTCGATTTTATTCGAGATACCCGGCACTTGCGTGCTCAAGAAAAGTCCGAAGAAGAGGAGGAATTAGAAGAGTTTGTTAATTTTGAGCGTTATCGGGATTTAATTAAGCATCGTCGTAGAGGATTTACCGATGATGAGGGTTTGCTACATGTGGATCAAGAGATAGAGGCCAAGTTTACTGCTCCTTTTGCTCCTAAGAGGTCTCAGGTGCAACAACCTTCTGCTGCTAAGGGTTCTTATTCGCAGGTGGGGTACTCTTATGAAGGGGATGGAAAAGATGAGGCCCAGTTTTCGGACCCTGATGATAACGATGAAGATGATGACGAAGATGAGGATGATGATGATTTCAACAGTGATGACAGCAGTGATGAAGGAATGGATAAAATAGCAAAAGAGTTTGGCGTAAAAAGATATGGTTGGCTTGTTTACATGGATAAGAAAGCTAAGGAGGAGGAGAAAAGGCAAAAAGAAGTGATCAAAGGCGATCCTGCAATTAGGAAGCTGAGTCGCAAGGAAAGAAGGAAGGCTTCTCAGATAGAGAGGGAGAGAGAAAGAGAAGCTGCACGTGTAACTGGAAGTAGGGTGCTCCACCATGACCCATACCGTGAAACTAGACGAAGTCCAACATATGAAGCTTACCCTCGCTCAAGAAGATCAAGATCGCGATCATATTCACCATCATACTCACGGAGATATCCTCGCGGACGACATTCTGATGATGCTCATCGAAGCAAACCAACAACTGCTAAAATAGAATATATTACTGAATTCGGGGGATCCGGGAACAAGGATGGCTCTAAGCGTGAAGGGTTCTCTCCACCGTCATCTCCTCCCTCACAGACCGATATGTTAAACCGGTCGGAGCACTCAACCTTTTATTCTTGCAAAAGATTCATTGGACACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRY_EERY

Protein Analysis

388

Amino Acids

45.25

Weight (kDa)

7.2

Isoelectric Point (pI)

72.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRY_EERY PF09750 41 - 174 2.3e-39 Alternative splicing regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 490
Acc36I ACCTGC 1 cut(s) 490
AccBSI CCGCTC 2 cut(s) 28, 56
AccII CGCG 4 cut(s) 102, 156, 919, 957
AciI CCGC 8 cut(s) 28, 54, 100, 102, 145, 154, 177, 957
AclWI GGATC 5 cut(s) 115, 417, 734, 1026, 1039
AcoI YGGCCR 2 cut(s) 90, 244
AcsI RAATTY 1 cut(s) 1022
AcvI CACGTG 1 cut(s) 821
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 152, 509
AfiI CCNNNNNNNGG 2 cut(s) 209, 547
AflIII ACRYGT 2 cut(s) 403, 820
AgeI ACCGGT 1 cut(s) 1110
AgsI TTSAA 1 cut(s) 604
AjiI CACGTC 1 cut(s) 77
AjnI CCWGG 2 cut(s) 87, 123
AleI CACNNNNGTG 1 cut(s) 285
AluBI AGCT 7 cut(s) 14, 68, 251, 698, 757, 815, 890
AluI AGCT 7 cut(s) 14, 68, 251, 698, 757, 815, 890
Alw21I GWGCWC 5 cut(s) 70, 253, 292, 843, 1122
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 5 cut(s) 115, 417, 734, 1026, 1039
AoxI GGCC 6 cut(s) 90, 163, 193, 244, 423, 537
ApeKI GCWGC 2 cut(s) 479, 815
ApoI RAATTY 1 cut(s) 1022
AsiGI ACCGGT 1 cut(s) 1110
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 4 cut(s) 34, 184, 538, 550
AsuC2I CCSGG 2 cut(s) 277, 1036
AsuHPI GGTGA 1 cut(s) 921
AvaII GGWCC 3 cut(s) 34, 184, 550
BalI TGGCCA 1 cut(s) 92
BamHI GGATCC 1 cut(s) 1031
BanII GRGCYC 2 cut(s) 70, 253
BbrPI CACGTG 1 cut(s) 821
Bbv12I GWGCWC 5 cut(s) 70, 253, 292, 843, 1122
BbvI GCAGC 2 cut(s) 466, 802
BccI CCATC 5 cut(s) 40, 236, 518, 940, 1040
BceAI ACGGC 2 cut(s) 65, 178
BciT130I CCWGG 2 cut(s) 89, 125
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 2 cut(s) 277, 1036
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 2 cut(s) 15, 867
BfoI RGCGCY 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 7 cut(s) 54, 100, 103, 145, 177, 480, 816
BlsI GCNGC 7 cut(s) 55, 101, 104, 146, 178, 481, 817
Bme1390I CCNGG 4 cut(s) 89, 125, 277, 1036
Bme18I GGWCC 3 cut(s) 34, 184, 550
BmgBI CACGTC 1 cut(s) 77
BmgT120I GGNCC 4 cut(s) 34, 184, 538, 550
BmiI GGNNCC 3 cut(s) 136, 552, 1033
BmrFI CCNGG 4 cut(s) 89, 125, 277, 1036
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 2 cut(s) 373, 964
BmuI ACTGGG 1 cut(s) 535
BplI GAGNNNNNCTC 4 cut(s) 240, 272, 937, 969
Bpu10I CCTNAGC 2 cut(s) 483, 699
BpuEI CTTGAG 2 cut(s) 276, 886
BpuMI CCSGG 2 cut(s) 277, 1036
Bsa29I ATCGAT 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 821
BsaBI GATNNNNATC 3 cut(s) 913, 919, 978
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 100, 180, 203
BsaWI WCCGGW 1 cut(s) 1110
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 547
Bse118I RCCGGY 1 cut(s) 1110
Bse1I ACTGG 2 cut(s) 541, 832
Bse8I GATNNNNATC 3 cut(s) 913, 919, 978
BseBI CCWGG 2 cut(s) 89, 125
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 100, 180, 203
BseGI GGATG 4 cut(s) 226, 529, 595, 1051
BseJI GATNNNNATC 3 cut(s) 913, 919, 978
BseLI CCNNNNNNNGG 2 cut(s) 209, 547
BseMII CTCAG 3 cut(s) 473, 749, 800
BseNI ACTGG 2 cut(s) 541, 832
BseRI GAGGAG 4 cut(s) 326, 719, 722, 1074
BseXI GCAGC 2 cut(s) 466, 802
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 4 cut(s) 102, 156, 919, 957
Bsh1285I CGRYCG 1 cut(s) 1115
BshFI GGCC 6 cut(s) 92, 165, 195, 246, 425, 539
BshTI ACCGGT 1 cut(s) 1110
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 1115
BsiHKAI GWGCWC 5 cut(s) 70, 253, 292, 843, 1122
BsiSI CCGG 3 cut(s) 277, 1035, 1111
BslI CCNNNNNNNGG 2 cut(s) 209, 547
BsmAI GTCTC 1 cut(s) 462
BsnI GGCC 6 cut(s) 92, 165, 195, 246, 425, 539
Bso31I GGTCTC 1 cut(s) 462
Bsp1286I GDGCHC 5 cut(s) 70, 253, 292, 843, 1122
Bsp68I TCGCGA 1 cut(s) 919
BspACI CCGC 8 cut(s) 28, 54, 100, 102, 145, 154, 177, 957
BspANI GGCC 6 cut(s) 92, 165, 195, 246, 425, 539
BspCNI CTCAG 3 cut(s) 472, 750, 799
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 4 cut(s) 102, 156, 919, 957
BspLI GGNNCC 3 cut(s) 136, 552, 1033
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 5 cut(s) 115, 417, 734, 1026, 1039
BspTNI GGTCTC 1 cut(s) 462
BsrBI CCGCTC 2 cut(s) 28, 56
BsrFI RCCGGY 1 cut(s) 1110
BsrI ACTGG 2 cut(s) 541, 832
BssAI RCCGGY 1 cut(s) 1110
BssECI CCNNGG 3 cut(s) 100, 180, 203
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 203
Bst2UI CCWGG 2 cut(s) 89, 125
Bst4CI ACNGT 3 cut(s) 608, 860, 1077
Bst6I CTCTTC 2 cut(s) 303, 318
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 821
BstC8I GCNNGC 2 cut(s) 66, 288
BstDEI CTNAG 7 cut(s) 450, 459, 483, 699, 758, 786, 1053
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 4 cut(s) 226, 529, 595, 1051
BstFNI CGCG 4 cut(s) 102, 156, 919, 957
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstMAI GTCTC 1 cut(s) 462
BstMCI CGRYCG 1 cut(s) 1115
BstMWI GCNNNNNNNGC 4 cut(s) 141, 153, 162, 443
BstNI CCWGG 2 cut(s) 89, 125
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 4 cut(s) 87, 123, 275, 1034
BstUI CGCG 4 cut(s) 102, 156, 919, 957
BstV1I GCAGC 2 cut(s) 466, 802
BstX2I RGATCY 1 cut(s) 1031
BstYI RGATCY 1 cut(s) 1031
Bsu15I ATCGAT 1 cut(s) 231
BsuRI GGCC 6 cut(s) 92, 165, 195, 246, 425, 539
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 171
BtrI CACGTC 1 cut(s) 77
BtsCI GGATG 4 cut(s) 226, 529, 595, 1051
BtsI GCAGTG 1 cut(s) 625
BtsIMutI CAGTG 2 cut(s) 613, 625
BtuMI TCGCGA 1 cut(s) 919
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 66, 288
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 1 cut(s) 1110
Cfr13I GGNCC 4 cut(s) 34, 184, 538, 550
Cfr42I CCGCGG 1 cut(s) 103
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 1 cut(s) 59
Csp6I GTAC 2 cut(s) 151, 508
CspAI ACCGGT 1 cut(s) 1110
CviAII CATG 4 cut(s) 43, 404, 685, 848
CviQI GTAC 2 cut(s) 151, 508
DdeI CTNAG 7 cut(s) 450, 459, 483, 699, 758, 786, 1053
EaeI YGGCCR 2 cut(s) 90, 244
Eam1104I CTCTTC 2 cut(s) 303, 318
EarI CTCTTC 2 cut(s) 303, 318
Ecl136II GAGCTC 2 cut(s) 68, 251
Eco130I CCWWGG 1 cut(s) 203
Eco147I AGGCCT 1 cut(s) 195
Eco24I GRGCYC 2 cut(s) 70, 253
Eco31I GGTCTC 1 cut(s) 462
Eco32I GATATC 1 cut(s) 950
Eco47I GGWCC 3 cut(s) 34, 184, 550
Eco53kI GAGCTC 2 cut(s) 68, 251
Eco72I CACGTG 1 cut(s) 821
EcoICRI GAGCTC 2 cut(s) 68, 251
EcoRI GAATTC 1 cut(s) 1022
EcoRII CCWGG 2 cut(s) 87, 123
EcoRV GATATC 1 cut(s) 950
EcoT14I CCWWGG 1 cut(s) 203
EcoT38I GRGCYC 2 cut(s) 70, 253
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 4 cut(s) 46, 407, 688, 851
FalI AAGNNNNNCTT 2 cut(s) 766, 798
FatI CATG 4 cut(s) 42, 403, 684, 847
FauNDI CATATG 1 cut(s) 883
FbaI TGATCA 1 cut(s) 729
Fnu4HI GCNGC 7 cut(s) 54, 100, 103, 145, 177, 480, 816
FokI GGATG 4 cut(s) 233, 536, 602, 1058
FriOI GRGCYC 2 cut(s) 70, 253
Fsp4HI GCNGC 7 cut(s) 54, 100, 103, 145, 177, 480, 816
FspBI CTAG 2 cut(s) 15, 867
GlaI GCGC 1 cut(s) 98
GluI GCNGC 7 cut(s) 54, 100, 103, 145, 177, 480, 816
GsaI CCCAGC 1 cut(s) 115
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 6 cut(s) 92, 165, 195, 246, 425, 539
HapII CCGG 3 cut(s) 277, 1035, 1111
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 4 cut(s) 46, 407, 688, 851
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 3 cut(s) 199, 760, 1146
HpaII CCGG 3 cut(s) 277, 1035, 1111
HphI GGTGA 1 cut(s) 921
Hpy166II GTNNAC 3 cut(s) 37, 432, 682
Hpy188I TCNGA 7 cut(s) 25, 228, 304, 550, 789, 970, 1117
Hpy188III TCNNGA 8 cut(s) 269, 293, 350, 413, 903, 912, 918, 1161
Hpy8I GTNNAC 3 cut(s) 37, 432, 682
Hpy99I CGWCG 3 cut(s) 78, 134, 372
HpyAV CCTTC 7 cut(s) 25, 483, 512, 620, 768, 772, 1055
HpyCH4III ACNGT 3 cut(s) 608, 860, 1077
HpyCH4IV ACGT 2 cut(s) 76, 820
HpyCH4V TGCA 4 cut(s) 466, 746, 818, 1140
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 153, 162, 443
HpyF3I CTNAG 7 cut(s) 450, 459, 483, 699, 758, 786, 1053
HpySE526I ACGT 2 cut(s) 76, 820
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 4 cut(s) 46, 407, 688, 851
HspAI GCGC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 729
KspI CCGCGG 1 cut(s) 103
LmnI GCTCC 6 cut(s) 25, 140, 442, 451, 846, 1117
Lsp1109I GCAGC 2 cut(s) 466, 802
LweI GCATC 2 cut(s) 373, 964
MaeI CTAG 2 cut(s) 15, 867
MaeII ACGT 2 cut(s) 76, 820
MaeIII GTNAC 1 cut(s) 823
MbiI CCGCTC 2 cut(s) 28, 56
MboII GAAGA 6 cut(s) 317, 320, 335, 581, 593, 918
MflI RGATCY 1 cut(s) 1031
MhlI GDGCHC 5 cut(s) 70, 253, 292, 843, 1122
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 5 cut(s) 317, 334, 357, 747, 1022
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 1 cut(s) 769
MmeI TCCRAC 2 cut(s) 902, 1095
Mox20I TGGCCA 1 cut(s) 92
MscI TGGCCA 1 cut(s) 92
MseI TTAA 4 cut(s) 333, 356, 360, 1106
MslI CAYNNNNRTG 2 cut(s) 285, 969
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 3 cut(s) 277, 1035, 1111
MspR9I CCNGG 4 cut(s) 89, 125, 277, 1036
MvaI CCWGG 2 cut(s) 89, 125
MvnI CGCG 4 cut(s) 102, 156, 919, 957
MwoI GCNNNNNNNGC 4 cut(s) 141, 153, 162, 443
NciI CCSGG 2 cut(s) 277, 1036
NdeI CATATG 1 cut(s) 883
NlaIII CATG 4 cut(s) 46, 407, 688, 851
NlaIV GGNNCC 3 cut(s) 136, 552, 1033
NmeAIII GCCGAG 2 cut(s) 205, 272
NruI TCGCGA 1 cut(s) 919
NspI RCATGY 1 cut(s) 407
OliI CACNNNNGTG 1 cut(s) 285
PacI TTAATTAA 1 cut(s) 360
PaqCI CACCTGC 1 cut(s) 490
PceI AGGCCT 1 cut(s) 195
PciI ACATGT 1 cut(s) 403
PfeI GAWTC 2 cut(s) 199, 1146
PflFI GACNNNGTC 1 cut(s) 873
PfoI TCCNGGA 1 cut(s) 1034
PinAI ACCGGT 1 cut(s) 1110
PkrI GCNGC 7 cut(s) 55, 101, 104, 146, 178, 481, 817
PleI GAGTC 1 cut(s) 768
PmaCI CACGTG 1 cut(s) 821
PmlI CACGTG 1 cut(s) 821
PpsI GAGTC 1 cut(s) 768
Ppu21I YACGTR 1 cut(s) 821
PscI ACATGT 1 cut(s) 403
Psp124BI GAGCTC 2 cut(s) 70, 253
Psp6I CCWGG 2 cut(s) 87, 123
PspCI CACGTG 1 cut(s) 821
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 2 cut(s) 87, 123
PspN4I GGNNCC 3 cut(s) 136, 552, 1033
PspPI GGNCC 4 cut(s) 34, 184, 538, 550
PsuI RGATCY 1 cut(s) 1031
PsyI GACNNNGTC 1 cut(s) 873
RruI TCGCGA 1 cut(s) 919
RsaI GTAC 2 cut(s) 152, 509
RsaNI GTAC 2 cut(s) 151, 508
RseI CAYNNNNRTG 2 cut(s) 285, 969
SacI GAGCTC 2 cut(s) 70, 253
SacII CCGCGG 1 cut(s) 103
SaqAI TTAA 4 cut(s) 333, 356, 360, 1106
SatI GCNGC 7 cut(s) 54, 100, 103, 145, 177, 480, 816
Sau96I GGNCC 4 cut(s) 34, 184, 538, 550
SchI GAGTC 1 cut(s) 769
ScrFI CCNGG 4 cut(s) 89, 125, 277, 1036
SduI GDGCHC 5 cut(s) 70, 253, 292, 843, 1122
SfaNI GCATC 2 cut(s) 373, 964
Sfr303I CCGCGG 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 103
SinI GGWCC 3 cut(s) 34, 184, 550
SmiMI CAYNNNNRTG 2 cut(s) 285, 969
SmlI CTYRAG 2 cut(s) 291, 901
SmoI CTYRAG 2 cut(s) 291, 901
Sse9I AATT 5 cut(s) 317, 334, 357, 747, 1022
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 8 cut(s) 28, 54, 100, 102, 145, 154, 177, 957
SspMI CTAG 2 cut(s) 15, 867
SstI GAGCTC 2 cut(s) 70, 253
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 4 cut(s) 87, 123, 275, 1034
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 3 cut(s) 608, 860, 1077
TaiI ACGT 2 cut(s) 79, 823
TaqI TCGA 6 cut(s) 58, 119, 231, 258, 268, 982
TaqII GACCGA 1 cut(s) 1112
TasI AATT 5 cut(s) 317, 334, 357, 747, 1022
TauI GCSGC 5 cut(s) 56, 102, 105, 147, 179
TfiI GAWTC 2 cut(s) 199, 1146
Tru1I TTAA 4 cut(s) 333, 356, 360, 1106
Tru9I TTAA 4 cut(s) 333, 356, 360, 1106
TscAI CASTG 2 cut(s) 613, 625
TseI GCWGC 2 cut(s) 479, 815
TspDTI ATGAA 5 cut(s) 531, 582, 639, 900, 1138
TspGWI ACGGA 1 cut(s) 958
TspRI CASTG 2 cut(s) 613, 625
Tth111I GACNNNGTC 1 cut(s) 873
VpaK11BI GGWCC 3 cut(s) 34, 184, 550
XapI RAATTY 1 cut(s) 1022
XceI RCATGY 1 cut(s) 407
XspI CTAG 2 cut(s) 15, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.