Rh2BG189900

CLK4-associating serine arginine rich

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004786
Physical Location & Seq
Forward (+)
17061123 .. 17065231
4109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG189900.1

Sequence Viewer

Length: 1182 bp
ATGTGGCACGAAGCGAGGAGGTCGGAGAGGAAAGTCCACGACATGATGGACGCCGCTCGCAAACGAGCTCAACGACGTGCCGTTTTCCTCGCCAAACGCCGCGGCGATCCCCAGCAGTCCATCCAGGTCGTCGGCTCCCGCTGCCGCCTCTACCGCGACGACGGCCTTTATCAAGCCACCGAGGATCAGCAAGGCCTGATTCCTTGGAATGGAAAACAGGATGTGTTAATTGACAGATTTGATGGGCGTGCGCTTCTCGATTTTATTCGAGAGTCTAAGCATTTTCGTGTTCAAGAGAAGTCGGAAGAGGAGGAAGAGTTAGAAGAGTTTGTTAATTTTGAGCGTTATCGGGATTTAATTAAGCATCGTCGTAGAGGATTTACTGATGATGAGGGTTTGCTACACGTGAATCAAGAGATGGAGGCCAAGGTTACTGCTCCGTTTGCACCTGAGAGGTCTCAGCCGCAACAAGCTTCTGCCACCAAGGGTTCTTATTCCCAAGTTGGGTTCTCTTATGGTGGGGATGGAAAAGAGGACGCCCAGTATTCAGATGCTGATGAAGACGATGACGATGATGACGATGACGAAGATGAGGATGATTTCAACTCTGATGATAGCAGTGATGAAGGAATGGATAAAATAGCAAAAGAATTTGGAATCAAGAGGTATGGTTGGCTTGTCTACATGGATAAGAAAGCCAAGGAGGAGGAGAAAAGGCAAAAGGAAGTGATCAAAGGCGATCCTGCAATTAGGAAGATGAGTCGCAAGGAAAGAAGGAAGGTTTCTCAGATAGAAAGGGAGAGAGAAAGAGAAACTGCCCGGGTAACCGGAACTCGTGTTCTCCAACATGATCCATACCGTGAAAGCAGGCGAAGTCCAACATATGAAGCTTATCCTCGTTCAAGAAGGTACAGTGTCTATATCATACAATGTATATCCTTAACTTCCCATCAAAGGCATTTAGTAGATGAATGTTATCTTGTGTGTGCAGATCAAGATCTCGATCACCATCATACTCAAGGCGTCATCCTCGCGGACGACATTCTGATGCTCATAGAAGCAAACCAACGACTTCTAAAATTGAATACATTACAGAATTTGGGGGCTCAGGCGATAAGGAAGAATCAAAGCGTGAAGGGTTTTCTCCACCACAATCTCCTCGCTCACAGACTGATGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRY_EERY

Protein Analysis

393

Amino Acids

46.11

Weight (kDa)

5.93

Isoelectric Point (pI)

55.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRY_EERY PF09750 41 - 174 6.7e-41 Alternative splicing regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 56
AccI GTMKAC 1 cut(s) 681
AccII CGCG 3 cut(s) 102, 156, 1034
AciI CCGC 8 cut(s) 54, 100, 102, 139, 145, 154, 464, 1034
AclWI GGATC 4 cut(s) 101, 192, 734, 845
AcsI RAATTY 2 cut(s) 650, 1096
AcvI CACGTG 1 cut(s) 406
AcyI GRCGYC 3 cut(s) 51, 537, 1023
AfaI GTAC 1 cut(s) 911
AfiI CCNNNNNNNGG 3 cut(s) 209, 504, 954
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 4 cut(s) 293, 604, 903, 1084
AjiI CACGTC 1 cut(s) 77
AjnI CCWGG 1 cut(s) 123
AloI GAACNNNNNNTCC 2 cut(s) 822, 854
AluBI AGCT 3 cut(s) 68, 473, 890
AluI AGCT 3 cut(s) 68, 473, 890
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 4 cut(s) 101, 192, 734, 845
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 819
AoxI GGCC 3 cut(s) 163, 193, 423
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 2 cut(s) 650, 1096
AspLEI GCGC 1 cut(s) 253
AsuC2I CCSGG 2 cut(s) 820, 821
AsuHPI GGTGA 1 cut(s) 998
AvaI CYCGRG 1 cut(s) 819
BanII GRGCYC 2 cut(s) 70, 1108
BauI CACGAG 1 cut(s) 834
BbrPI CACGTG 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 128
BccI CCATC 7 cut(s) 40, 128, 236, 412, 518, 957, 1017
BceAI ACGGC 2 cut(s) 65, 178
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 2 cut(s) 820, 821
BcoDI GTCTC 1 cut(s) 462
BglII AGATCT 1 cut(s) 997
BisI GCNGC 6 cut(s) 54, 100, 103, 142, 145, 464
BlsI GCNGC 6 cut(s) 55, 101, 104, 143, 146, 465
Bme1390I CCNGG 3 cut(s) 125, 820, 821
BmeT110I CYCGRG 1 cut(s) 819
BmgBI CACGTC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 3 cut(s) 125, 820, 821
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 373, 541, 1038
BmuI ACTGGG 1 cut(s) 535
BpiI GAAGAC 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 1107
BpuEI CTTGAG 1 cut(s) 1002
BpuMI CCSGG 2 cut(s) 820, 821
BsaAI YACGTR 1 cut(s) 406
BsaBI GATNNNNATC 3 cut(s) 996, 1002, 1008
BsaHI GRCGYC 3 cut(s) 51, 537, 1023
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 7 cut(s) 100, 180, 203, 426, 483, 699, 819
BsaWI WCCGGW 1 cut(s) 827
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 504, 954
Bse1I ACTGG 1 cut(s) 541
Bse8I GATNNNNATC 3 cut(s) 996, 1002, 1008
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 7 cut(s) 100, 180, 203, 426, 483, 699, 819
BseGI GGATG 5 cut(s) 120, 226, 529, 601, 1026
BseJI GATNNNNATC 3 cut(s) 996, 1002, 1008
BseLI CCNNNNNNNGG 3 cut(s) 209, 504, 954
BseMII CTCAG 4 cut(s) 441, 473, 800, 1121
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 5 cut(s) 31, 323, 719, 722, 1148
BseXI GCAGC 1 cut(s) 128
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 1 cut(s) 1008
Bsh1236I CGCG 3 cut(s) 102, 156, 1034
BshFI GGCC 3 cut(s) 165, 195, 425
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 819
BsiSI CCGG 2 cut(s) 820, 828
BslI CCNNNNNNNGG 3 cut(s) 209, 504, 954
BsmAI GTCTC 1 cut(s) 462
BsnI GGCC 3 cut(s) 165, 195, 425
Bso31I GGTCTC 1 cut(s) 462
BsoBI CYCGRG 1 cut(s) 819
Bsp1286I GDGCHC 2 cut(s) 70, 1108
Bsp143I GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
BspACI CCGC 8 cut(s) 54, 100, 102, 139, 145, 154, 464, 1034
BspANI GGCC 3 cut(s) 165, 195, 425
BspCNI CTCAG 4 cut(s) 442, 472, 799, 1120
BspFNI CGCG 3 cut(s) 102, 156, 1034
BspLI GGNNCC 1 cut(s) 136
BspPI GGATC 4 cut(s) 101, 192, 734, 845
BspTNI GGTCTC 1 cut(s) 462
BsrBI CCGCTC 1 cut(s) 56
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 7 cut(s) 100, 180, 203, 426, 483, 699, 819
BssMI GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
BssNI GRCGYC 3 cut(s) 51, 537, 1023
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 4 cut(s) 203, 426, 483, 699
Bst2BI CACGAG 1 cut(s) 834
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 860, 914
Bst6I CTCTTC 3 cut(s) 300, 309, 318
BstACI GRCGYC 3 cut(s) 51, 537, 1023
BstBAI YACGTR 1 cut(s) 406
BstC8I GCNNGC 3 cut(s) 58, 249, 869
BstDEI CTNAG 5 cut(s) 276, 450, 459, 786, 1107
BstDSI CCRYGG 1 cut(s) 100
BstEII GGTNACC 1 cut(s) 823
BstF5I GGATG 5 cut(s) 120, 226, 529, 601, 1026
BstFNI CGCG 3 cut(s) 102, 156, 1034
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 8 cut(s) 109, 187, 732, 742, 853, 994, 1000, 1006
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 141, 153, 162, 443
BstNI CCWGG 1 cut(s) 125
BstPI GGTNACC 1 cut(s) 823
BstSCI CCNGG 3 cut(s) 123, 818, 819
BstUI CGCG 3 cut(s) 102, 156, 1034
BstV1I GCAGC 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 567
BstX2I RGATCY 1 cut(s) 997
BstYI RGATCY 1 cut(s) 997
BsuRI GGCC 3 cut(s) 165, 195, 425
BtgI CCRYGG 1 cut(s) 100
BtrI CACGTC 1 cut(s) 77
BtsCI GGATG 5 cut(s) 120, 226, 529, 601, 1026
BtsI GCAGTG 1 cut(s) 625
BtsIMutI CAGTG 2 cut(s) 625, 919
Cac8I GCNNGC 3 cut(s) 58, 249, 869
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 1 cut(s) 253
Cfr42I CCGCGG 1 cut(s) 103
Cfr9I CCCGGG 1 cut(s) 819
CseI GACGC 3 cut(s) 59, 545, 1012
Csp6I GTAC 1 cut(s) 910
CviAII CATG 3 cut(s) 43, 685, 848
CviQI GTAC 1 cut(s) 910
DdeI CTNAG 5 cut(s) 276, 450, 459, 786, 1107
DpnI GATC 8 cut(s) 108, 186, 731, 741, 852, 993, 999, 1005
DpnII GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
Eam1104I CTCTTC 3 cut(s) 300, 309, 318
EarI CTCTTC 3 cut(s) 300, 309, 318
Ecl136II GAGCTC 1 cut(s) 68
Eco130I CCWWGG 4 cut(s) 203, 426, 483, 699
Eco147I AGGCCT 1 cut(s) 195
Eco24I GRGCYC 2 cut(s) 70, 1108
Eco31I GGTCTC 1 cut(s) 462
Eco53kI GAGCTC 1 cut(s) 68
Eco72I CACGTG 1 cut(s) 406
Eco88I CYCGRG 1 cut(s) 819
Eco91I GGTNACC 1 cut(s) 823
EcoICRI GAGCTC 1 cut(s) 68
EcoO65I GGTNACC 1 cut(s) 823
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 4 cut(s) 203, 426, 483, 699
EcoT38I GRGCYC 2 cut(s) 70, 1108
ErhI CCWWGG 4 cut(s) 203, 426, 483, 699
FaeI CATG 3 cut(s) 46, 688, 851
FatI CATG 3 cut(s) 42, 684, 847
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 883
FbaI TGATCA 1 cut(s) 729
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 6 cut(s) 54, 100, 103, 142, 145, 464
FokI GGATG 5 cut(s) 107, 233, 536, 608, 1013
FriOI GRGCYC 2 cut(s) 70, 1108
Fsp4HI GCNGC 6 cut(s) 54, 100, 103, 142, 145, 464
GlaI GCGC 1 cut(s) 252
GluI GCNGC 6 cut(s) 54, 100, 103, 142, 145, 464
GsaI CCCAGC 1 cut(s) 115
HaeIII GGCC 3 cut(s) 165, 195, 425
HapII CCGG 2 cut(s) 820, 828
HgaI GACGC 3 cut(s) 59, 545, 1012
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 3 cut(s) 51, 537, 1023
Hin1II CATG 3 cut(s) 46, 688, 851
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HindIII AAGCTT 2 cut(s) 471, 888
HinfI GANTC 6 cut(s) 199, 272, 409, 657, 760, 1123
HpaII CCGG 2 cut(s) 820, 828
HphI GGTGA 1 cut(s) 998
Hpy166II GTNNAC 2 cut(s) 37, 682
Hpy188I TCNGA 6 cut(s) 25, 304, 550, 610, 789, 1047
Hpy188III TCNNGA 9 cut(s) 257, 269, 293, 350, 413, 661, 903, 995, 1001
Hpy8I GTNNAC 2 cut(s) 37, 682
Hpy99I CGWCG 5 cut(s) 78, 134, 161, 164, 372
HpyAV CCTTC 5 cut(s) 620, 768, 772, 900, 1129
HpyCH4III ACNGT 2 cut(s) 860, 914
HpyCH4IV ACGT 2 cut(s) 76, 405
HpyCH4V TGCA 3 cut(s) 446, 746, 989
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 153, 162, 443
HpyF3I CTNAG 5 cut(s) 276, 450, 459, 786, 1107
HpySE526I ACGT 2 cut(s) 76, 405
Hsp92I GRCGYC 3 cut(s) 51, 537, 1023
Hsp92II CATG 3 cut(s) 46, 688, 851
HspAI GCGC 1 cut(s) 251
Ksp22I TGATCA 1 cut(s) 729
KspI CCGCGG 1 cut(s) 103
Kzo9I GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
LmnI GCTCC 2 cut(s) 140, 442
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 3 cut(s) 373, 541, 1038
MaeII ACGT 2 cut(s) 76, 405
MaeIII GTNAC 2 cut(s) 430, 823
MalI GATC 8 cut(s) 108, 186, 731, 741, 852, 993, 999, 1005
MbiI CCGCTC 1 cut(s) 56
MboI GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
MboII GAAGA 7 cut(s) 317, 326, 335, 572, 599, 766, 1132
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 2 cut(s) 70, 1108
MluCI AATT 7 cut(s) 228, 334, 357, 650, 747, 1079, 1096
MlyI GAGTC 2 cut(s) 281, 769
MmeI TCCRAC 3 cut(s) 282, 868, 902
MseI TTAA 6 cut(s) 227, 333, 356, 360, 941, 1180
MslI CAYNNNNRTG 2 cut(s) 285, 1046
MspA1I CMGCKG 2 cut(s) 102, 141
MspI CCGG 2 cut(s) 820, 828
MspR9I CCNGG 3 cut(s) 125, 820, 821
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 3 cut(s) 102, 156, 1034
MwoI GCNNNNNNNGC 4 cut(s) 141, 153, 162, 443
NciI CCSGG 2 cut(s) 820, 821
NdeI CATATG 1 cut(s) 883
NdeII GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
NlaIII CATG 3 cut(s) 46, 688, 851
NlaIV GGNNCC 1 cut(s) 136
PacI TTAATTAA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 2 cut(s) 70, 576
PfeI GAWTC 4 cut(s) 199, 409, 657, 1123
PkrI GCNGC 6 cut(s) 55, 101, 104, 143, 146, 465
PleI GAGTC 2 cut(s) 280, 768
PmaCI CACGTG 1 cut(s) 406
PmlI CACGTG 1 cut(s) 406
PpsI GAGTC 2 cut(s) 280, 768
Ppu21I YACGTR 1 cut(s) 406
Psp124BI GAGCTC 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 123
PspCI CACGTG 1 cut(s) 406
PspEI GGTNACC 1 cut(s) 823
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 997
RsaI GTAC 1 cut(s) 911
RsaNI GTAC 1 cut(s) 910
RseI CAYNNNNRTG 2 cut(s) 285, 1046
SacI GAGCTC 1 cut(s) 70
SacII CCGCGG 1 cut(s) 103
SaqAI TTAA 6 cut(s) 227, 333, 356, 360, 941, 1180
SatI GCNGC 6 cut(s) 54, 100, 103, 142, 145, 464
Sau3AI GATC 8 cut(s) 106, 184, 729, 739, 850, 991, 997, 1003
SchI GAGTC 2 cut(s) 281, 769
ScrFI CCNGG 3 cut(s) 125, 820, 821
SduI GDGCHC 2 cut(s) 70, 1108
SfaNI GCATC 3 cut(s) 373, 541, 1038
Sfr303I CCGCGG 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 103
SmaI CCCGGG 1 cut(s) 821
SmiMI CAYNNNNRTG 2 cut(s) 285, 1046
SmlI CTYRAG 1 cut(s) 1017
SmoI CTYRAG 1 cut(s) 1017
Sse9I AATT 7 cut(s) 228, 334, 357, 650, 747, 1079, 1096
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 8 cut(s) 54, 100, 102, 139, 145, 154, 464, 1034
SstI GAGCTC 1 cut(s) 70
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 3 cut(s) 123, 818, 819
StyI CCWWGG 4 cut(s) 203, 426, 483, 699
TaaI ACNGT 2 cut(s) 860, 914
TaiI ACGT 2 cut(s) 79, 408
TaqI TCGA 3 cut(s) 258, 268, 1002
TasI AATT 7 cut(s) 228, 334, 357, 650, 747, 1079, 1096
TauI GCSGC 5 cut(s) 56, 102, 105, 147, 466
TfiI GAWTC 4 cut(s) 199, 409, 657, 1123
Tru1I TTAA 6 cut(s) 227, 333, 356, 360, 941, 1180
Tru9I TTAA 6 cut(s) 227, 333, 356, 360, 941, 1180
TscAI CASTG 2 cut(s) 625, 919
TseI GCWGC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 573, 639, 900, 984
TspGWI ACGGA 1 cut(s) 429
TspMI CCCGGG 1 cut(s) 819
TspRI CASTG 2 cut(s) 625, 919
XapI RAATTY 2 cut(s) 650, 1096
XmaI CCCGGG 1 cut(s) 819
XmiI GTMKAC 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.