MD15G1284200.v1.1

CLK4-associating serine arginine rich

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004786
Physical Location & Seq
Forward (+)
25742496 .. 25743311
816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1284200.v1.1.491

Sequence Viewer

Length: 432 bp
ATGTGGCACGAAGCCAGGAGGTCGGAGCGGAAGGTCCACGACATGATGGACGCCGCTCGTAAACGAGCTCAAAGACGTGCCGTTTTCCTGGCCAAGCGCCGCGGCGACCCCCAGCAGTCGATCCAGGTCGTCGGCTCTCGCTGCCGCATGTGCCGCGATGACGGCCTCTACCAAGCCGCCGAGGACCAGCAAGGCCTGATTCCTTGGAACGGGAAGCAGGATGTTTTGATTGATAGATTTGATTGGCGAGCTCTGCTCGATTTTATTCGAGATACGCCGCACTTGCGTGCTCAAGAACAGTCCGAAGAAGAGGAGGAATTAGAAGAGTGTGTTAATTTTGAGTGTTATCGGGATTTAATTAAGCATCGTCGTAGAGGATGTCGGTACTTTGTTTTGATTTCTAATCATATGTATTCATTTAAATGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRY_EERY

Protein Analysis

144

Amino Acids

17.25

Weight (kDa)

8.58

Isoelectric Point (pI)

70.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRY_EERY PF09750 41 - 127 1.4e-27 Alternative splicing regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 28, 56
AccII CGCG 2 cut(s) 102, 156
AciI CCGC 8 cut(s) 28, 54, 100, 102, 145, 154, 177, 278
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 1 cut(s) 209
AjiI CACGTC 1 cut(s) 77
AjnI CCWGG 3 cut(s) 14, 87, 123
AleI CACNNNNGTG 1 cut(s) 285
AluBI AGCT 2 cut(s) 68, 251
AluI AGCT 2 cut(s) 68, 251
Alw21I GWGCWC 3 cut(s) 70, 253, 292
AlwI GGATC 1 cut(s) 115
AoxI GGCC 3 cut(s) 90, 163, 193
ApeKI GCWGC 1 cut(s) 141
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 34, 184
AvaII GGWCC 2 cut(s) 34, 184
BalI TGGCCA 1 cut(s) 92
BanII GRGCYC 2 cut(s) 70, 253
Bbv12I GWGCWC 3 cut(s) 70, 253, 292
BbvI GCAGC 1 cut(s) 128
BccI CCATC 1 cut(s) 40
BceAI ACGGC 2 cut(s) 65, 178
BciT130I CCWGG 3 cut(s) 16, 89, 125
BfoI RGCGCY 1 cut(s) 100
BisI GCNGC 8 cut(s) 54, 100, 103, 142, 145, 154, 177, 278
BlsI GCNGC 8 cut(s) 55, 101, 104, 143, 146, 155, 178, 279
Bme1390I CCNGG 3 cut(s) 16, 89, 125
Bme18I GGWCC 2 cut(s) 34, 184
BmgBI CACGTC 1 cut(s) 77
BmgT120I GGNCC 2 cut(s) 34, 184
BmrFI CCNGG 3 cut(s) 16, 89, 125
BmsI GCATC 1 cut(s) 373
BplI GAGNNNNNCTC 2 cut(s) 240, 272
BpuEI CTTGAG 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 100, 180, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 209
BseBI CCWGG 3 cut(s) 16, 89, 125
BseDI CCNNGG 3 cut(s) 100, 180, 203
BseGI GGATG 2 cut(s) 226, 383
BseLI CCNNNNNNNGG 1 cut(s) 209
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 1 cut(s) 128
BseYI CCCAGC 1 cut(s) 111
Bsh1236I CGCG 2 cut(s) 102, 156
BshFI GGCC 3 cut(s) 92, 165, 195
BsiHKAI GWGCWC 3 cut(s) 70, 253, 292
BslI CCNNNNNNNGG 1 cut(s) 209
BsnI GGCC 3 cut(s) 92, 165, 195
Bsp1286I GDGCHC 3 cut(s) 70, 253, 292
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 8 cut(s) 28, 54, 100, 102, 145, 154, 177, 278
BspANI GGCC 3 cut(s) 92, 165, 195
BspFNI CGCG 2 cut(s) 102, 156
BspPI GGATC 1 cut(s) 115
BsrBI CCGCTC 2 cut(s) 28, 56
BssECI CCNNGG 3 cut(s) 100, 180, 203
BssMI GATC 1 cut(s) 120
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 203
Bst2UI CCWGG 3 cut(s) 16, 89, 125
Bst4CI ACNGT 1 cut(s) 300
Bst6I CTCTTC 2 cut(s) 303, 318
BstACI GRCGYC 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 249, 288
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 226, 383
BstFNI CGCG 2 cut(s) 102, 156
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 1 cut(s) 123
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 6 cut(s) 141, 150, 153, 162, 253, 283
BstNI CCWGG 3 cut(s) 16, 89, 125
BstNSI RCATGY 1 cut(s) 151
BstSCI CCNGG 3 cut(s) 14, 87, 123
BstUI CGCG 2 cut(s) 102, 156
BstV1I GCAGC 1 cut(s) 128
BsuRI GGCC 3 cut(s) 92, 165, 195
BtgI CCRYGG 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 171
BtrI CACGTC 1 cut(s) 77
BtsCI GGATG 2 cut(s) 226, 383
Cac8I GCNNGC 2 cut(s) 249, 288
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 2 cut(s) 34, 184
Cfr42I CCGCGG 1 cut(s) 103
CseI GACGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 385
CviAII CATG 2 cut(s) 43, 148
CviJI RGCY 8 cut(s) 14, 68, 92, 135, 165, 176, 195, 251
CviKI_1 RGCY 8 cut(s) 14, 68, 92, 135, 165, 176, 195, 251
CviQI GTAC 1 cut(s) 385
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
DraI TTTAAA 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 303, 318
EarI CTCTTC 2 cut(s) 303, 318
Ecl136II GAGCTC 2 cut(s) 68, 251
Eco130I CCWWGG 1 cut(s) 203
Eco147I AGGCCT 1 cut(s) 195
Eco24I GRGCYC 2 cut(s) 70, 253
Eco47I GGWCC 2 cut(s) 34, 184
Eco53kI GAGCTC 2 cut(s) 68, 251
EcoICRI GAGCTC 2 cut(s) 68, 251
EcoRII CCWGG 3 cut(s) 14, 87, 123
EcoT14I CCWWGG 1 cut(s) 203
EcoT38I GRGCYC 2 cut(s) 70, 253
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 2 cut(s) 46, 151
FaiI YATR 5 cut(s) 44, 149, 408, 410, 430
FatI CATG 2 cut(s) 42, 147
FauNDI CATATG 1 cut(s) 408
Fnu4HI GCNGC 8 cut(s) 54, 100, 103, 142, 145, 154, 177, 278
FokI GGATG 2 cut(s) 233, 390
FriOI GRGCYC 2 cut(s) 70, 253
Fsp4HI GCNGC 8 cut(s) 54, 100, 103, 142, 145, 154, 177, 278
GlaI GCGC 1 cut(s) 98
GluI GCNGC 8 cut(s) 54, 100, 103, 142, 145, 154, 177, 278
GsaI CCCAGC 1 cut(s) 115
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 3 cut(s) 92, 165, 195
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 99
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 2 cut(s) 46, 151
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 37, 62
Hpy188I TCNGA 2 cut(s) 25, 304
Hpy188III TCNNGA 3 cut(s) 269, 293, 350
Hpy8I GTNNAC 2 cut(s) 37, 62
Hpy99I CGWCG 2 cut(s) 134, 372
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4IV ACGT 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 150, 153, 162, 253, 283
HpySE526I ACGT 1 cut(s) 76
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 2 cut(s) 46, 151
HspAI GCGC 1 cut(s) 97
KspI CCGCGG 1 cut(s) 103
Kzo9I GATC 1 cut(s) 120
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 9 cut(s) 28, 74, 101, 110, 125, 137, 200, 203, 209
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 373
MaeII ACGT 1 cut(s) 76
MalI GATC 1 cut(s) 122
MbiI CCGCTC 2 cut(s) 28, 56
MboI GATC 1 cut(s) 120
MboII GAAGA 3 cut(s) 317, 320, 335
MhlI GDGCHC 3 cut(s) 70, 253, 292
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 3 cut(s) 317, 334, 357
MluNI TGGCCA 1 cut(s) 92
MnlI CCTC 6 cut(s) 12, 175, 176, 304, 307, 368
Mox20I TGGCCA 1 cut(s) 92
MscI TGGCCA 1 cut(s) 92
MseI TTAA 4 cut(s) 333, 356, 360, 420
MslI CAYNNNNRTG 2 cut(s) 285, 421
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 102
MspR9I CCNGG 3 cut(s) 16, 89, 125
MvaI CCWGG 3 cut(s) 16, 89, 125
MvnI CGCG 2 cut(s) 102, 156
MwoI GCNNNNNNNGC 6 cut(s) 141, 150, 153, 162, 253, 283
NdeI CATATG 1 cut(s) 408
NdeII GATC 1 cut(s) 120
NlaIII CATG 2 cut(s) 46, 151
NmeAIII GCCGAG 1 cut(s) 205
NspI RCATGY 1 cut(s) 151
OliI CACNNNNGTG 1 cut(s) 285
PacI TTAATTAA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 195
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 8 cut(s) 55, 101, 104, 143, 146, 155, 178, 279
Psp124BI GAGCTC 2 cut(s) 70, 253
Psp6I CCWGG 3 cut(s) 14, 87, 123
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 3 cut(s) 14, 87, 123
PspPI GGNCC 2 cut(s) 34, 184
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 2 cut(s) 285, 421
SacI GAGCTC 2 cut(s) 70, 253
SacII CCGCGG 1 cut(s) 103
SaqAI TTAA 4 cut(s) 333, 356, 360, 420
SatI GCNGC 8 cut(s) 54, 100, 103, 142, 145, 154, 177, 278
Sau3AI GATC 1 cut(s) 120
Sau96I GGNCC 2 cut(s) 34, 184
ScrFI CCNGG 3 cut(s) 16, 89, 125
SduI GDGCHC 3 cut(s) 70, 253, 292
SetI ASST 6 cut(s) 23, 36, 70, 79, 129, 253
SfaNI GCATC 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 103
SinI GGWCC 2 cut(s) 34, 184
SmiI ATTTAAAT 1 cut(s) 421
SmiMI CAYNNNNRTG 2 cut(s) 285, 421
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
Sse9I AATT 3 cut(s) 317, 334, 357
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 8 cut(s) 28, 54, 100, 102, 145, 154, 177, 278
SstI GAGCTC 2 cut(s) 70, 253
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 3 cut(s) 14, 87, 123
StyI CCWWGG 1 cut(s) 203
SwaI ATTTAAAT 1 cut(s) 421
TaaI ACNGT 1 cut(s) 300
TaiI ACGT 1 cut(s) 79
TaqI TCGA 3 cut(s) 119, 258, 268
TasI AATT 3 cut(s) 317, 334, 357
TauI GCSGC 7 cut(s) 56, 102, 105, 147, 156, 179, 280
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 4 cut(s) 333, 356, 360, 420
Tru9I TTAA 4 cut(s) 333, 356, 360, 420
TseI GCWGC 1 cut(s) 141
TspDTI ATGAA 1 cut(s) 405
VpaK11BI GGWCC 2 cut(s) 34, 184
XceI RCATGY 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.