MD02G1190600.v1.1

GTP-BINDING protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005373
Physical Location & Seq
Forward (+)
17602558 .. 17606714
4157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1190600.v1.1.491

Sequence Viewer

Length: 1785 bp
ATGCTGAGAACCATCTCTCTCTCGCGGAATCCTCTGAGGTCCTTCTCCCAAATCCTAACCCCTCTCACTTCAACTTCCACGCCAAACCCAACCCCATCACCGCCTTCGATTCCGACTTCCCACTCCTTCGGCTCCTCCTCCTCCTCCTCGTCGAAGCAAGACAAGGAGGAAAAAACCGAGGCCGTTTCGGTTTTCAACAGAGATCCAACAGTCCCTCCCAAGCTCTTCGTCGTCCAGCCCCGCCTCCGCCCCGATACCTTCTTGCAGGCCAAGCTCAACGAAGCTCTCTGCCTCGCCAATTCGCTTGAAGAACAGCGAGATGGGTATTTCGATATCGATTTTTTCGATAAGGAAATTCCTCCCCATGTTGTTGTTCAAAACCCCATTGTTCGACGCCAAACCCGCGCAGATACATATTTTGGAAAAGGGACTTTGGATGTTGTGAAATGCCATCTGAACGCTGTGGAGCAAAAGGGTGAAGTAGATGCTGTATTTATAAATACAATTCTGTCTGGGATTCAGCAGAGGAATTTGGAGAAAATTTTGGGAAAGGCGGTGTTGGACCGCGTGGGTCTTATAATAGAGATATTCAATGCTCATGCCTGCACAAAGGAGGGAAAGCTACAGGCTGAATTAGCGGCTCTAATGTATAAGAAAACAAGACTTGTACGACTGCGTGGCAAAGCTGGACGCTCTACTTTTGGAATGGATGGAGAAGCTGAAGTTGTTAGTGCCCGAGGGAGAGGAAGTGGGGGACGTGGTTTCATTAGTGGTGCAGGAGAAACTGAACTTCAGCTTCAACGACGAAGAATTTCAGAAAGGAGAAATAGTCTGTTATCGCAAATTCAAGATGTTCGTCGTACTCGAGCTGTGCAACGCGCTGGTCGCAAGAATCATGGAGGATCTTATGGTCAAGGTTTACCTACTGTTGCTGTTGTTGGGTATACAAATGCTGGGAAATCTACATTAGTTAGTGCCATCTCAAACAGTGATCTATACAGTGATTCTCGATTGTTTGCTACAGTTGATCCAAAATTGAGAAGTGTTGTTCTTCCTTCAGGGAAGAAAGTGCTCGTGAGTGACACGGTCGGATTTATATCAGATTTGCCAGTGCAGCTAGTGGAAGCATTTCATGCAACTTTAGAAGAAGTTGTGGAAGCTGATCTGCTTGTGCATGTGTTGGATTGTACGGCACCTAATCTTGATGAGCATCGATCAACTGTATTACAAGTTCTTGAACAAATTGGCGTATCCAAGGAAAAGCTTGAAAACATGATAGAAGTTTGGAATAAGATTGACCATGAGGAGGACGGTGCAGATTTTAATGAGTGTCTTGTTGACGGTGAAGATGGTGAGACTAGCAAATTCTCAGGAGAAGAAGATATGGAATGTGAGTTCTCAGGATCAGAAAATGATCTGTCATCAGAGAATCTTGAAGCTGTGGATGACCAAGAAGGTGATTACTCTGATGGTTGGTTGGCATCAGAAGATGTCGAAGATCCTTGGGGGCGAGGTGGGTCATGTTCAGCCTTGAAAACTACGGATGACCAACAAAGTGAATATCCTAAGGACTGGGGAACAGAAAAGCAAGTGCAATCACCAGTTCAGTCCGGTCCACATGTAAGAACATCTGCCTTAATGGGAGTTGGCTTGCAAGAGTTGATGGAGCTCATTGATGAGAAGCTGAAGAAGCTCCCAAAGGAAAATGTTGTGGAAAGGGGTACATTTGATCGGAAATGGAGGCCCCCTCGTACAGAGGAGGCCGCCATAGCAGTTGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043021 GO:0043022 GO:0044424 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

595

Amino Acids

65.47

Weight (kDa)

5.16

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_N PF13167 128 - 197 2.5e-12 GTP-binding GTPase N-terminal
GTP-bdg_M PF16360 200 - 296 1.3e-13 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 309 - 431 2.5e-16 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 497, 578
AccB1I GGYRCC 1 cut(s) 1192
AccI GTMKAC 1 cut(s) 944
AccII CGCG 4 cut(s) 25, 405, 567, 879
AciI CCGC 9 cut(s) 25, 101, 241, 247, 403, 554, 565, 638, 1764
AclWI GGATC 5 cut(s) 197, 910, 1022, 1411, 1493
AcsI RAATTY 6 cut(s) 354, 529, 540, 810, 843, 1364
AcuI CTGAAG 4 cut(s) 741, 776, 1041, 1706
AcyI GRCGYC 1 cut(s) 394
AfaI GTAC 5 cut(s) 669, 862, 1189, 1723, 1753
AfiI CCNNNNNNNGG 1 cut(s) 1306
AflIII ACRYGT 1 cut(s) 1618
AjiI CACGTC 1 cut(s) 758
AloI GAACNNNNNNTCC 2 cut(s) 1379, 1411
Alw21I GWGCWC 2 cut(s) 1074, 1671
Alw26I GTCTC 1 cut(s) 1349
AlwI GGATC 5 cut(s) 197, 910, 1022, 1411, 1493
Ama87I CYCGRG 2 cut(s) 735, 864
AoxI GGCC 4 cut(s) 180, 267, 1742, 1761
ApeKI GCWGC 1 cut(s) 1114
ApoI RAATTY 6 cut(s) 354, 529, 540, 810, 843, 1364
Asp700I GAANNNNTTC 2 cut(s) 811, 1128
AspLEI GCGC 2 cut(s) 407, 881
AspS9I GGNCC 4 cut(s) 39, 562, 1613, 1743
AsuHPI GGTGA 6 cut(s) 90, 488, 1355, 1364, 1469, 1590
AvaI CYCGRG 2 cut(s) 735, 864
AvaII GGWCC 3 cut(s) 39, 562, 1613
AxyI CCTNAGG 1 cut(s) 1566
BaeGI GKGCMC 1 cut(s) 736
BanI GGYRCC 1 cut(s) 1192
BanII GRGCYC 1 cut(s) 1671
BauI CACGAG 1 cut(s) 1073
Bbv12I GWGCWC 2 cut(s) 1074, 1671
BbvI GCAGC 1 cut(s) 1126
BccI CCATC 9 cut(s) 20, 103, 314, 459, 704, 986, 1343, 1463, 1657
BceAI ACGGC 2 cut(s) 167, 1206
BciVI GTATCC 1 cut(s) 1261
BcoDI GTCTC 1 cut(s) 1349
BfaI CTAG 2 cut(s) 1118, 1359
BfmI CTRYAG 2 cut(s) 623, 1020
BfuI GTATCC 1 cut(s) 1261
BisI GCNGC 3 cut(s) 639, 1115, 1764
BlsI GCNGC 3 cut(s) 640, 1116, 1765
Bme18I GGWCC 3 cut(s) 39, 562, 1613
BmeT110I CYCGRG 2 cut(s) 735, 864
BmgBI CACGTC 1 cut(s) 758
BmgT120I GGNCC 4 cut(s) 39, 562, 1613, 1743
BmiI GGNNCC 3 cut(s) 133, 1194, 1745
BmrI ACTGGG 1 cut(s) 1582
BmsI GCATC 3 cut(s) 475, 1219, 1490
BmuI ACTGGG 1 cut(s) 1582
BplI GAGNNNNNCTC 2 cut(s) 1732, 1764
Bsa29I ATCGAT 2 cut(s) 336, 1213
BsaBI GATNNNNATC 2 cut(s) 1096, 1209
BsaHI GRCGYC 1 cut(s) 394
BsaJI CCNNGG 4 cut(s) 177, 736, 1254, 1502
BsaWI WCCGGW 1 cut(s) 1610
BsaXI ACNNNNNCTCC 6 cut(s) 199, 229, 733, 763, 814, 844
Bsc4I CCNNNNNNNGG 1 cut(s) 1306
Bse1I ACTGG 3 cut(s) 1109, 1577, 1601
Bse21I CCTNAGG 1 cut(s) 1566
Bse8I GATNNNNATC 2 cut(s) 1096, 1209
BseCI ATCGAT 2 cut(s) 336, 1213
BseDI CCNNGG 4 cut(s) 177, 736, 1254, 1502
BseGI GGATG 4 cut(s) 442, 715, 1450, 1549
BseJI GATNNNNATC 2 cut(s) 1096, 1209
BseLI CCNNNNNNNGG 1 cut(s) 1306
BseMII CTCAG 3 cut(s) 26, 1383, 1413
BseNI ACTGG 3 cut(s) 1109, 1577, 1601
BseRI GAGGAG 7 cut(s) 124, 127, 130, 133, 136, 1319, 1772
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 1 cut(s) 1126
BseYI CCCAGC 1 cut(s) 953
BsgI GTGCAG 4 cut(s) 589, 795, 1133, 1335
Bsh1236I CGCG 4 cut(s) 25, 405, 567, 879
Bsh1285I CGRYCG 1 cut(s) 1089
BshFI GGCC 4 cut(s) 182, 269, 1744, 1763
BshNI GGYRCC 1 cut(s) 1192
BshVI ATCGAT 2 cut(s) 336, 1213
BsiEI CGRYCG 1 cut(s) 1089
BsiHKAI GWGCWC 2 cut(s) 1074, 1671
BsiHKCI CYCGRG 2 cut(s) 735, 864
BsiSI CCGG 1 cut(s) 1611
BslFI GGGAC 3 cut(s) 197, 442, 768
BslI CCNNNNNNNGG 1 cut(s) 1306
BsmAI GTCTC 1 cut(s) 1349
BsmFI GGGAC 3 cut(s) 197, 442, 768
BsnI GGCC 4 cut(s) 182, 269, 1744, 1763
BsoBI CYCGRG 2 cut(s) 735, 864
Bsp1286I GDGCHC 3 cut(s) 736, 1074, 1671
BspACI CCGC 9 cut(s) 25, 101, 241, 247, 403, 554, 565, 638, 1764
BspANI GGCC 4 cut(s) 182, 269, 1744, 1763
BspCNI CTCAG 3 cut(s) 27, 1382, 1412
BspDI ATCGAT 2 cut(s) 336, 1213
BspFNI CGCG 4 cut(s) 25, 405, 567, 879
BspLI GGNNCC 3 cut(s) 133, 1194, 1745
BspPI GGATC 5 cut(s) 197, 910, 1022, 1411, 1493
BspQI GCTCTTC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 1192
BsrI ACTGG 3 cut(s) 1109, 1577, 1601
BssECI CCNNGG 4 cut(s) 177, 736, 1254, 1502
BssNAI GTATAC 1 cut(s) 945
BssNI GRCGYC 1 cut(s) 394
BssSI CACGAG 1 cut(s) 1073
BssT1I CCWWGG 2 cut(s) 1254, 1502
Bst1107I GTATAC 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 1073
Bst4CI ACNGT 9 cut(s) 211, 928, 989, 1001, 1024, 1087, 1222, 1313, 1343
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 394
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstC8I GCNNGC 3 cut(s) 267, 604, 1652
BstDEI CTNAG 5 cut(s) 5, 35, 1369, 1399, 1566
BstF5I GGATG 4 cut(s) 442, 715, 1450, 1549
BstFNI CGCG 4 cut(s) 25, 405, 567, 879
BstHHI GCGC 2 cut(s) 407, 881
BstMAI GTCTC 1 cut(s) 1349
BstMCI CGRYCG 1 cut(s) 1089
BstMWI GCNNNNNNNGC 8 cut(s) 271, 402, 635, 885, 1114, 1133, 1690, 1769
BstNSI RCATGY 2 cut(s) 1178, 1622
BstSFI CTRYAG 2 cut(s) 623, 1020
BstSLI GKGCMC 1 cut(s) 736
BstUI CGCG 4 cut(s) 25, 405, 567, 879
BstV1I GCAGC 1 cut(s) 1126
BstX2I RGATCY 3 cut(s) 202, 902, 1498
BstYI RGATCY 3 cut(s) 202, 902, 1498
BstZ17I GTATAC 1 cut(s) 945
Bsu15I ATCGAT 2 cut(s) 336, 1213
Bsu36I CCTNAGG 1 cut(s) 1566
BsuI GTATCC 1 cut(s) 1261
BsuRI GGCC 4 cut(s) 182, 269, 1744, 1763
BsuTUI ATCGAT 2 cut(s) 336, 1213
BtrI CACGTC 1 cut(s) 758
BtsCI GGATG 4 cut(s) 442, 715, 1450, 1549
BtsIMutI CAGTG 3 cut(s) 994, 1006, 1116
Cac8I GCNNGC 3 cut(s) 267, 604, 1652
CfoI GCGC 2 cut(s) 407, 881
Cfr13I GGNCC 4 cut(s) 39, 562, 1613, 1743
ClaI ATCGAT 2 cut(s) 336, 1213
CseI GACGC 2 cut(s) 402, 699
Csp6I GTAC 5 cut(s) 668, 861, 1188, 1722, 1752
CviAII CATG 9 cut(s) 365, 599, 896, 1133, 1175, 1273, 1301, 1521, 1619
CviQI GTAC 5 cut(s) 668, 861, 1188, 1722, 1752
DdeI CTNAG 5 cut(s) 5, 35, 1369, 1399, 1566
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 1669
Eco130I CCWWGG 2 cut(s) 1254, 1502
Eco24I GRGCYC 1 cut(s) 1671
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 3 cut(s) 39, 562, 1613
Eco53kI GAGCTC 1 cut(s) 1669
Eco57I CTGAAG 4 cut(s) 741, 776, 1041, 1706
Eco81I CCTNAGG 1 cut(s) 1566
Eco88I CYCGRG 2 cut(s) 735, 864
EcoICRI GAGCTC 1 cut(s) 1669
EcoO109I RGGNCCY 2 cut(s) 39, 1743
EcoRV GATATC 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 1254, 1502
EcoT38I GRGCYC 1 cut(s) 1671
ErhI CCWWGG 2 cut(s) 1254, 1502
FaeI CATG 9 cut(s) 368, 602, 899, 1136, 1178, 1276, 1304, 1524, 1622
FaqI GGGAC 3 cut(s) 197, 442, 768
FatI CATG 9 cut(s) 364, 598, 895, 1132, 1174, 1272, 1300, 1520, 1618
FauI CCCGC 2 cut(s) 248, 410
FblI GTMKAC 1 cut(s) 944
Fnu4HI GCNGC 3 cut(s) 639, 1115, 1764
FokI GGATG 4 cut(s) 449, 722, 1457, 1556
FriOI GRGCYC 1 cut(s) 1671
Fsp4HI GCNGC 3 cut(s) 639, 1115, 1764
FspBI CTAG 2 cut(s) 1118, 1359
GlaI GCGC 2 cut(s) 406, 880
GluI GCNGC 3 cut(s) 639, 1115, 1764
GsaI CCCAGC 1 cut(s) 957
HaeIII GGCC 4 cut(s) 182, 269, 1744, 1763
HapII CCGG 1 cut(s) 1611
HgaI GACGC 2 cut(s) 402, 699
HhaI GCGC 2 cut(s) 407, 881
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 9 cut(s) 368, 602, 899, 1136, 1178, 1276, 1304, 1524, 1622
Hin6I GCGC 2 cut(s) 405, 879
HinP1I GCGC 2 cut(s) 405, 879
HincII GTYRAC 1 cut(s) 1339
HindII GTYRAC 1 cut(s) 1339
HindIII AAGCTT 1 cut(s) 1262
HinfI GANTC 6 cut(s) 28, 109, 517, 892, 1004, 1429
HpaII CCGG 1 cut(s) 1611
HphI GGTGA 6 cut(s) 90, 488, 1355, 1364, 1469, 1590
Hpy166II GTNNAC 4 cut(s) 920, 945, 1339, 1616
Hpy188III TCNNGA 8 cut(s) 848, 1008, 1075, 1202, 1235, 1371, 1401, 1433
Hpy8I GTNNAC 4 cut(s) 920, 945, 1339, 1616
Hpy99I CGWCG 5 cut(s) 154, 233, 396, 807, 861
HpyAV CCTTC 6 cut(s) 52, 114, 136, 268, 1065, 1448
HpyCH4III ACNGT 9 cut(s) 211, 928, 989, 1001, 1024, 1087, 1222, 1313, 1343
HpyCH4IV ACGT 1 cut(s) 757
HpyF10VI GCNNNNNNNGC 8 cut(s) 271, 402, 635, 885, 1114, 1133, 1690, 1769
HpyF3I CTNAG 5 cut(s) 5, 35, 1369, 1399, 1566
HpySE526I ACGT 1 cut(s) 757
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 9 cut(s) 368, 602, 899, 1136, 1178, 1276, 1304, 1524, 1622
HspAI GCGC 2 cut(s) 405, 879
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 4 cut(s) 137, 466, 1666, 1698
Lsp1109I GCAGC 1 cut(s) 1126
LweI GCATC 3 cut(s) 475, 1219, 1490
MaeI CTAG 2 cut(s) 1118, 1359
MaeII ACGT 1 cut(s) 757
MaeIII GTNAC 1 cut(s) 1079
MflI RGATCY 3 cut(s) 202, 902, 1498
MhlI GDGCHC 3 cut(s) 736, 1074, 1671
MmeI TCCRAC 5 cut(s) 137, 230, 540, 1069, 1161
MroXI GAANNNNTTC 2 cut(s) 811, 1128
MseI TTAA 2 cut(s) 1323, 1637
MspI CCGG 1 cut(s) 1611
MvnI CGCG 4 cut(s) 25, 405, 567, 879
MwoI GCNNNNNNNGC 8 cut(s) 271, 402, 635, 885, 1114, 1133, 1690, 1769
NlaIII CATG 9 cut(s) 368, 602, 899, 1136, 1178, 1276, 1304, 1524, 1622
NlaIV GGNNCC 3 cut(s) 133, 1194, 1745
NmuCI GTSAC 1 cut(s) 1079
NspI RCATGY 2 cut(s) 1178, 1622
PaeR7I CTCGAG 1 cut(s) 864
PciI ACATGT 1 cut(s) 1618
PciSI GCTCTTC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 862
PdmI GAANNNNTTC 2 cut(s) 811, 1128
PfeI GAWTC 6 cut(s) 28, 109, 517, 892, 1004, 1429
PflFI GACNNNGTC 1 cut(s) 1085
PkrI GCNGC 3 cut(s) 640, 1116, 1765
PpuMI RGGWCCY 1 cut(s) 39
PscI ACATGT 1 cut(s) 1618
PsiI TTATAA 2 cut(s) 497, 578
Psp124BI GAGCTC 1 cut(s) 1671
Psp5II RGGWCCY 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 953
PspN4I GGNNCC 3 cut(s) 133, 1194, 1745
PspPI GGNCC 4 cut(s) 39, 562, 1613, 1743
PspPPI RGGWCCY 1 cut(s) 39
PspXI VCTCGAGB 1 cut(s) 864
PsrI GAACNNNNNNTAC 2 cut(s) 1215, 1247
PsuI RGATCY 3 cut(s) 202, 902, 1498
PsyI GACNNNGTC 1 cut(s) 1085
RsaI GTAC 5 cut(s) 669, 862, 1189, 1723, 1753
RsaNI GTAC 5 cut(s) 668, 861, 1188, 1722, 1752
SacI GAGCTC 1 cut(s) 1671
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 2 cut(s) 1323, 1637
SatI GCNGC 3 cut(s) 639, 1115, 1764
Sau96I GGNCC 4 cut(s) 39, 562, 1613, 1743
SduI GDGCHC 3 cut(s) 736, 1074, 1671
SfaNI GCATC 3 cut(s) 475, 1219, 1490
SfcI CTRYAG 2 cut(s) 623, 1020
Sfr274I CTCGAG 1 cut(s) 864
SinI GGWCC 3 cut(s) 39, 562, 1613
SlaI CTCGAG 1 cut(s) 864
SmlI CTYRAG 1 cut(s) 864
SmoI CTYRAG 1 cut(s) 864
SsiI CCGC 9 cut(s) 25, 101, 241, 247, 403, 554, 565, 638, 1764
SspMI CTAG 2 cut(s) 1118, 1359
SstI GAGCTC 1 cut(s) 1671
StyI CCWWGG 2 cut(s) 1254, 1502
TaaI ACNGT 9 cut(s) 211, 928, 989, 1001, 1024, 1087, 1222, 1313, 1343
TaiI ACGT 1 cut(s) 760
TauI GCSGC 2 cut(s) 641, 1766
TfiI GAWTC 6 cut(s) 28, 109, 517, 892, 1004, 1429
Tru1I TTAA 2 cut(s) 1323, 1637
Tru9I TTAA 2 cut(s) 1323, 1637
TscAI CASTG 3 cut(s) 994, 1006, 1116
TseFI GTSAC 1 cut(s) 1079
TseI GCWGC 1 cut(s) 1114
Tsp45I GTSAC 1 cut(s) 1079
TspDTI ATGAA 2 cut(s) 754, 1121
TspGWI ACGGA 1 cut(s) 1556
TspRI CASTG 3 cut(s) 994, 1006, 1116
Tth111I GACNNNGTC 1 cut(s) 1085
VpaK11BI GGWCC 3 cut(s) 39, 562, 1613
XapI RAATTY 6 cut(s) 354, 529, 540, 810, 843, 1364
XceI RCATGY 2 cut(s) 1178, 1622
XhoI CTCGAG 1 cut(s) 864
XmiI GTMKAC 1 cut(s) 944
XmnI GAANNNNTTC 2 cut(s) 811, 1128
XspI CTAG 2 cut(s) 1118, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.