Prupe.6G215400_v2.0.a1

GTP-BINDING protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0005373
Physical Location & Seq
Reverse (-)
22297212 .. 22301063
3852 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G215400.1

Sequence Viewer

Length: 1704 bp
ATGCTGAGAACCATTTCTCTCTCACGGAGTACTCTGAGGTCCTTCTCCCAAACCCGCACCCATCTGTGTTCAAACCCAAGCCAAACCCCATCTCCACCTTCAACCCCTTCGATTTTGATTTCCTACTCTTTTTACTCGACGTCATCGAAGCAAGAAAAGGAGGACAAAAACGACGTTGCTTCGGTTTTCAACAGGGACCCAACAGTCCCTCCCAAGCTCTTCGTGGTCCAGCCTCGTCTTCGCCCCGATAACTACTTGCAGGCCAAGCTCAACGAGGCTCTCTGCCTCGCCAACTCTCTTGAAGAACAGCGAGATGGGTACTTCGATGTCGATTTTTTCGATAAGGAAATACCTCCCCACATTGTTGTTCAAAACCCAATTGTTAGACACCAAACTCGAGCAGTGGATGCTATATTTGTGAATACAATTCTTTCTGGGATTCAGCAGAGGAATTTGGAGAAAATTTTGGGAAAACCAGTCTTGGACCGTGTGGGTCTTATAATAGAGATATTCAATGCTCATGCCTGCACAAAGGAGGGCAAGCTGCAGGCTGAATTAGCAGCTCTAATGTATAAGAAAACAAGACTTGTACGACTGCGTGGCAAAAGTGGACGCTCAACTTTTGGAACGGATGGAGAAGCTGAAGTTGTTAGTGCCCGAGGGAGAGGAAGTGGGGGACGTGGCTTCATTAGTGGTGCAGGAGAAACTGAACTTCAGCTTCAACGACGAAGAATTTCAGAACGGAGGAGTAGTCTTTTACTGCAAATTCAAGAGGTTCGACGAACTCGAGCTGTGCAACGTGCTGGTCGGAAGAATCGTGGAGGCTCATATGGTCAAGGTTTACCTACTGTTGCTGTTGTTGGGTATACAAATGCTGGGAAATCTACATTAGTTAGTGCCCTCTCAAATAGCGATCTTTACAGTGATTCTCGATTGTTCGCTACGGTTGATCCTAAATTGAGAAGTGTTTTTCTTCCTTCAGGGAAGAAAGTGCTTGTAAGTGACACAGTAGGATTTATATCAGATTTGCCAGTGCAGTTAGTGGAAGCATTTCATGCAACTCTAGAAGAAGTTGTGGAAGCTGATCTCCTTGTGCATGTGTTGGATTGTACTGCTCCCAATCTAAATGAGCACCGTTCAACTGTATTCCAAATTCTTGATCAAATCGGTGTATCAAAGGAAAAACTTGAAAACATGATTGAAGTTTGGAATAAGATTGACTATCAGGAGGAGGGCATGGATTTTGATGAGTGTGTTGATGACATTGAAGATGGTGAGGCTAGCAGTTTCTCAAGAGAAAAAGATGATATGGATTCTGAGCTCTCATCAGGAGCCGAAAACGATGAGGCTTTTGAAGCTATTGATGACCAAGATGGTGATTACTCTGACGGTTGGTTGGCATCAGAAGATGTCGAAGATCCTTGGGGTGAAGGTGGGTCCTGTATGGGGTTGAAGACTACAGACGACCAACAAAGCGAGTGTCCAAAGGACTGGATAACAGAAAAGCAATTGGAATTACAAGTTCAGTCTGGTCCACATGTTAGAACATCTGCTTTAATGGGAGTTGGGTTGCAAGAGTTGTTGGAACTCATTGATGAGAAGCTGAAGGAGGCCCCAAAGGCAAATGTTGTGGAAAGGGATCCATTTGATCGAAAATGGAGGCCTCCTCGGACAGAGGAGGCTGGCGTAGCAGTTGAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043021 GO:0043022 GO:0044424 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

568

Amino Acids

63.04

Weight (kDa)

5.0

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AasI GACNNNNNNGTC 1 cut(s) 203
AatII GACGTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 866
AciI CCGC 1 cut(s) 55
AclWI GGATC 4 cut(s) 944, 1412, 1634, 1647
AcsI RAATTY 5 cut(s) 451, 462, 732, 765, 1152
AcuI CTGAAG 4 cut(s) 663, 698, 963, 1625
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 4 cut(s) 31, 320, 591, 1111
AfiI CCNNNNNNNGG 1 cut(s) 1446
AflIII ACRYGT 1 cut(s) 1537
AjiI CACGTC 1 cut(s) 680
Alw21I GWGCWC 2 cut(s) 1134, 1323
AlwI GGATC 4 cut(s) 944, 1412, 1634, 1647
Ama87I CYCGRG 3 cut(s) 396, 657, 786
AoxI GGCC 3 cut(s) 261, 1611, 1661
ApeKI GCWGC 2 cut(s) 544, 560
ApoI RAATTY 5 cut(s) 451, 462, 732, 765, 1152
ArsI GACNNNNNNTTYG 2 cut(s) 1465, 1497
Asp700I GAANNNNTTC 3 cut(s) 13, 733, 1050
AspS9I GGNCC 7 cut(s) 39, 196, 226, 484, 1437, 1532, 1612
AsuHPI GGTGA 3 cut(s) 1286, 1388, 1439
AsuNHI GCTAGC 1 cut(s) 1280
AvaI CYCGRG 3 cut(s) 396, 657, 786
AvaII GGWCC 6 cut(s) 39, 196, 226, 484, 1437, 1532
BaeGI GKGCMC 2 cut(s) 658, 901
BamHI GGATCC 1 cut(s) 1639
BanII GRGCYC 1 cut(s) 1323
BbsI GAAGAC 2 cut(s) 230, 1460
Bbv12I GWGCWC 2 cut(s) 1134, 1323
BbvI GCAGC 2 cut(s) 531, 572
BccI CCATC 6 cut(s) 69, 97, 308, 626, 1265, 1367
BclI TGATCA 1 cut(s) 1159
BfaI CTAG 2 cut(s) 1064, 1281
BfmI CTRYAG 2 cut(s) 545, 1458
BglI GCCNNNNNGGC 1 cut(s) 1619
BisI GCNGC 2 cut(s) 545, 561
BlsI GCNGC 2 cut(s) 546, 562
BmcAI AGTACT 1 cut(s) 31
Bme18I GGWCC 6 cut(s) 39, 196, 226, 484, 1437, 1532
BmeT110I CYCGRG 3 cut(s) 396, 657, 786
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 7 cut(s) 39, 196, 226, 484, 1437, 1532, 1612
BmiI GGNNCC 6 cut(s) 197, 198, 1333, 1438, 1614, 1641
BmsI GCATC 2 cut(s) 397, 1409
BmtI GCTAGC 1 cut(s) 1284
BpiI GAAGAC 2 cut(s) 230, 1460
BplI GAGNNNNNCTC 2 cut(s) 1651, 1683
BpuEI CTTGAG 1 cut(s) 1276
BsaBI GATNNNNATC 1 cut(s) 1018
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 658, 1421, 1667
BsaXI ACNNNNNCTCC 8 cut(s) 76, 106, 193, 223, 655, 685, 736, 766
Bsc4I CCNNNNNNNGG 1 cut(s) 1446
Bse1I ACTGG 3 cut(s) 476, 1031, 1496
Bse8I GATNNNNATC 1 cut(s) 1018
BseDI CCNNGG 3 cut(s) 658, 1421, 1667
BseGI GGATG 2 cut(s) 412, 637
BseJI GATNNNNATC 1 cut(s) 1018
BseLI CCNNNNNNNGG 1 cut(s) 1446
BseMII CTCAG 2 cut(s) 26, 1308
BseNI ACTGG 3 cut(s) 476, 1031, 1496
BseRI GAGGAG 4 cut(s) 760, 1244, 1656, 1691
BseSI GKGCMC 2 cut(s) 658, 901
BseXI GCAGC 2 cut(s) 531, 572
BseYI CCCAGC 1 cut(s) 875
BsgI GTGCAG 3 cut(s) 511, 717, 1055
BshFI GGCC 3 cut(s) 263, 1613, 1663
BsiHKAI GWGCWC 2 cut(s) 1134, 1323
BsiHKCI CYCGRG 3 cut(s) 396, 657, 786
BslFI GGGAC 3 cut(s) 191, 209, 690
BslI CCNNNNNNNGG 1 cut(s) 1446
BsmFI GGGAC 3 cut(s) 191, 209, 690
BsnI GGCC 3 cut(s) 263, 1613, 1663
BsoBI CYCGRG 3 cut(s) 396, 657, 786
Bsp1286I GDGCHC 4 cut(s) 658, 901, 1134, 1323
Bsp143I GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
BspACI CCGC 1 cut(s) 55
BspANI GGCC 3 cut(s) 263, 1613, 1663
BspCNI CTCAG 2 cut(s) 27, 1309
BspLI GGNNCC 6 cut(s) 197, 198, 1333, 1438, 1614, 1641
BspMAI CTGCAG 1 cut(s) 549
BspOI GCTAGC 1 cut(s) 1284
BspPI GGATC 4 cut(s) 944, 1412, 1634, 1647
BspQI GCTCTTC 1 cut(s) 224
BsrI ACTGG 3 cut(s) 476, 1031, 1496
BssECI CCNNGG 3 cut(s) 658, 1421, 1667
BssMI GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
BssNAI GTATAC 1 cut(s) 867
BssNI GRCGYC 1 cut(s) 140
BssT1I CCWWGG 1 cut(s) 1421
Bst1107I GTATAC 1 cut(s) 867
Bst4CI ACNGT 9 cut(s) 205, 488, 850, 923, 946, 1009, 1136, 1144, 1391
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 1 cut(s) 140
BstAPI GCANNNNNTGC 2 cut(s) 407, 1055
BstC8I GCNNGC 6 cut(s) 261, 526, 542, 549, 1282, 1684
BstDEI CTNAG 3 cut(s) 5, 35, 1317
BstF5I GGATG 2 cut(s) 412, 637
BstKTI GATC 7 cut(s) 916, 952, 1087, 1162, 1420, 1642, 1651
BstMBI GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
BstMWI GCNNNNNNNGC 7 cut(s) 265, 407, 557, 1055, 1355, 1619, 1688
BstNSI RCATGY 2 cut(s) 1100, 1541
BstSFI CTRYAG 2 cut(s) 545, 1458
BstSLI GKGCMC 2 cut(s) 658, 901
BstV1I GCAGC 2 cut(s) 531, 572
BstV2I GAAGAC 2 cut(s) 230, 1460
BstX2I RGATCY 2 cut(s) 1417, 1639
BstXI CCANNNNNNTGG 1 cut(s) 1491
BstYI RGATCY 2 cut(s) 1417, 1639
BstZ17I GTATAC 1 cut(s) 867
BsuRI GGCC 3 cut(s) 263, 1613, 1663
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 2 cut(s) 412, 637
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 3 cut(s) 408, 928, 1038
Cac8I GCNNGC 6 cut(s) 261, 526, 542, 549, 1282, 1684
Cfr13I GGNCC 7 cut(s) 39, 196, 226, 484, 1437, 1532, 1612
CseI GACGC 1 cut(s) 621
Csp6I GTAC 4 cut(s) 30, 319, 590, 1110
CspCI CAANNNNNGTGG 2 cut(s) 1611, 1646
CviAII CATG 6 cut(s) 521, 1055, 1097, 1195, 1237, 1538
CviQI GTAC 4 cut(s) 30, 319, 590, 1110
DdeI CTNAG 3 cut(s) 5, 35, 1317
DpnI GATC 7 cut(s) 915, 951, 1086, 1161, 1419, 1641, 1650
DpnII GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Ecl136II GAGCTC 1 cut(s) 1321
Eco130I CCWWGG 1 cut(s) 1421
Eco147I AGGCCT 1 cut(s) 1663
Eco24I GRGCYC 1 cut(s) 1323
Eco47I GGWCC 6 cut(s) 39, 196, 226, 484, 1437, 1532
Eco53kI GAGCTC 1 cut(s) 1321
Eco57I CTGAAG 4 cut(s) 663, 698, 963, 1625
Eco88I CYCGRG 3 cut(s) 396, 657, 786
EcoICRI GAGCTC 1 cut(s) 1321
EcoO109I RGGNCCY 4 cut(s) 39, 196, 1437, 1612
EcoT14I CCWWGG 1 cut(s) 1421
EcoT38I GRGCYC 1 cut(s) 1323
ErhI CCWWGG 1 cut(s) 1421
FaeI CATG 6 cut(s) 524, 1058, 1100, 1198, 1240, 1541
FaqI GGGAC 3 cut(s) 191, 209, 690
FatI CATG 6 cut(s) 520, 1054, 1096, 1194, 1236, 1537
FauI CCCGC 1 cut(s) 62
FauNDI CATATG 1 cut(s) 829
FbaI TGATCA 1 cut(s) 1159
FblI GTMKAC 1 cut(s) 866
Fnu4HI GCNGC 2 cut(s) 545, 561
FokI GGATG 2 cut(s) 419, 644
FriOI GRGCYC 1 cut(s) 1323
Fsp4HI GCNGC 2 cut(s) 545, 561
FspBI CTAG 2 cut(s) 1064, 1281
GluI GCNGC 2 cut(s) 545, 561
GsaI CCCAGC 1 cut(s) 879
HaeIII GGCC 3 cut(s) 263, 1613, 1663
HgaI GACGC 1 cut(s) 621
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 6 cut(s) 524, 1058, 1100, 1198, 1240, 1541
HinfI GANTC 4 cut(s) 439, 814, 926, 1313
HphI GGTGA 3 cut(s) 1286, 1388, 1439
Hpy166II GTNNAC 4 cut(s) 611, 842, 867, 1535
Hpy188I TCNGA 8 cut(s) 36, 739, 810, 1024, 1318, 1387, 1405, 1671
Hpy188III TCNNGA 8 cut(s) 299, 770, 930, 1064, 1157, 1226, 1293, 1329
Hpy8I GTNNAC 4 cut(s) 611, 842, 867, 1535
Hpy99I CGWCG 4 cut(s) 142, 176, 729, 783
HpyAV CCTTC 6 cut(s) 52, 108, 117, 987, 1424, 1600
HpyCH4III ACNGT 9 cut(s) 205, 488, 850, 923, 946, 1009, 1136, 1144, 1391
HpyCH4IV ACGT 4 cut(s) 140, 174, 679, 799
HpyF10VI GCNNNNNNNGC 7 cut(s) 265, 407, 557, 1055, 1355, 1619, 1688
HpyF3I CTNAG 3 cut(s) 5, 35, 1317
HpySE526I ACGT 4 cut(s) 140, 174, 679, 799
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 6 cut(s) 524, 1058, 1100, 1198, 1240, 1541
KflI GGGWCCC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 1159
Kzo9I GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 2 cut(s) 1120, 1331
Lsp1109I GCAGC 2 cut(s) 531, 572
LweI GCATC 2 cut(s) 397, 1409
MaeI CTAG 2 cut(s) 1064, 1281
MaeII ACGT 4 cut(s) 140, 174, 679, 799
MaeIII GTNAC 1 cut(s) 1001
MalI GATC 7 cut(s) 915, 951, 1086, 1161, 1419, 1641, 1650
MboI GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
MfeI CAATTG 2 cut(s) 378, 1508
MflI RGATCY 2 cut(s) 1417, 1639
MhlI GDGCHC 4 cut(s) 658, 901, 1134, 1323
MmeI TCCRAC 3 cut(s) 788, 1083, 1563
MroXI GAANNNNTTC 3 cut(s) 13, 733, 1050
MseI TTAA 1 cut(s) 1556
MunI CAATTG 2 cut(s) 378, 1508
MwoI GCNNNNNNNGC 7 cut(s) 265, 407, 557, 1055, 1355, 1619, 1688
NdeI CATATG 1 cut(s) 829
NdeII GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
NheI GCTAGC 1 cut(s) 1280
NlaIII CATG 6 cut(s) 524, 1058, 1100, 1198, 1240, 1541
NlaIV GGNNCC 6 cut(s) 197, 198, 1333, 1438, 1614, 1641
NmuCI GTSAC 1 cut(s) 1001
NspI RCATGY 2 cut(s) 1100, 1541
PaeR7I CTCGAG 2 cut(s) 396, 786
PceI AGGCCT 1 cut(s) 1663
PciI ACATGT 1 cut(s) 1537
PciSI GCTCTTC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 4 cut(s) 143, 336, 784, 814
PdmI GAANNNNTTC 3 cut(s) 13, 733, 1050
PfeI GAWTC 4 cut(s) 439, 814, 926, 1313
PkrI GCNGC 2 cut(s) 546, 562
PpuMI RGGWCCY 3 cut(s) 39, 196, 1437
PscI ACATGT 1 cut(s) 1537
PsiI TTATAA 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 1323
Psp5II RGGWCCY 3 cut(s) 39, 196, 1437
PspFI CCCAGC 1 cut(s) 875
PspN4I GGNNCC 6 cut(s) 197, 198, 1333, 1438, 1614, 1641
PspPI GGNCC 7 cut(s) 39, 196, 226, 484, 1437, 1532, 1612
PspPPI RGGWCCY 3 cut(s) 39, 196, 1437
PspXI VCTCGAGB 2 cut(s) 396, 786
PstI CTGCAG 1 cut(s) 549
PsuI RGATCY 2 cut(s) 1417, 1639
RsaI GTAC 4 cut(s) 31, 320, 591, 1111
RsaNI GTAC 4 cut(s) 30, 319, 590, 1110
SacI GAGCTC 1 cut(s) 1323
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 1556
SatI GCNGC 2 cut(s) 545, 561
Sau3AI GATC 7 cut(s) 913, 949, 1084, 1159, 1417, 1639, 1648
Sau96I GGNCC 7 cut(s) 39, 196, 226, 484, 1437, 1532, 1612
ScaI AGTACT 1 cut(s) 31
SduI GDGCHC 4 cut(s) 658, 901, 1134, 1323
SfaNI GCATC 2 cut(s) 397, 1409
SfcI CTRYAG 2 cut(s) 545, 1458
Sfr274I CTCGAG 2 cut(s) 396, 786
SinI GGWCC 6 cut(s) 39, 196, 226, 484, 1437, 1532
SlaI CTCGAG 2 cut(s) 396, 786
SmlI CTYRAG 3 cut(s) 396, 786, 1291
SmoI CTYRAG 3 cut(s) 396, 786, 1291
SseBI AGGCCT 1 cut(s) 1663
SsiI CCGC 1 cut(s) 55
SspMI CTAG 2 cut(s) 1064, 1281
SstI GAGCTC 1 cut(s) 1323
StuI AGGCCT 1 cut(s) 1663
StyI CCWWGG 1 cut(s) 1421
TaaI ACNGT 9 cut(s) 205, 488, 850, 923, 946, 1009, 1136, 1144, 1391
TaiI ACGT 4 cut(s) 143, 177, 682, 802
TatI WGTACW 2 cut(s) 29, 1109
TfiI GAWTC 4 cut(s) 439, 814, 926, 1313
Tru1I TTAA 1 cut(s) 1556
Tru9I TTAA 1 cut(s) 1556
TscAI CASTG 3 cut(s) 408, 928, 1038
TseFI GTSAC 1 cut(s) 1001
TseI GCWGC 2 cut(s) 544, 560
Tsp45I GTSAC 1 cut(s) 1001
TspDTI ATGAA 2 cut(s) 676, 1043
TspGWI ACGGA 3 cut(s) 40, 644, 757
TspRI CASTG 3 cut(s) 408, 928, 1038
VpaK11BI GGWCC 6 cut(s) 39, 196, 226, 484, 1437, 1532
XapI RAATTY 5 cut(s) 451, 462, 732, 765, 1152
XbaI TCTAGA 1 cut(s) 1063
XceI RCATGY 2 cut(s) 1100, 1541
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XhoI CTCGAG 2 cut(s) 396, 786
XmiI GTMKAC 1 cut(s) 866
XmnI GAANNNNTTC 3 cut(s) 13, 733, 1050
XspI CTAG 2 cut(s) 1064, 1281
ZraI GACGTC 1 cut(s) 141
ZrmI AGTACT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.