RchiOBHm_Chr1g0321831

GTP-BINDING protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005373
Physical Location & Seq
Forward (+)
9354340 .. 9357642
3303 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55186

Sequence Viewer

Length: 1146 bp
ATGTATAAGAAAACAAGACTTATACGAACGCGTGGTCCAAGTGGACGCTTAGGTTTTGGAATGGGTGGAGAAGCCGAAGTTGTTAGCGCCCGAGGGAGAGGAAATGCTGGACCTGGATTCATTAGTGGGGCAGGAGAAACTGAACTTCAACTTCAACGACGAAGAATTTTAGAAAGGAGGAGTAGTCTGCTACAACAAATTAAGGATGTTCGTCGTACTCGAGCTGTGCAACGTGCTTCTCGCAAGAGGCATGGAAGCTCTGGTCAAGGCTTAGCTACTGTTGCTGTTGTGGGGTATACAAATGCTGGGAAATCTACATTGGTGAGTGCCCTCTCACATAGTGATCTCTACAGTGATTCTCGATTGTTTGCAACAGTTGACCCTAGATTGAGAAGTGTACTTCTTCCTTCAGGGAGGAAAGTGCTTGTTAGCGATACGGTAGGATTTATTTCAGATTTGCCAGTGCAGTTAGTGGAAGCTTTTCATGCGACTTTAGAAGAAGTTGTGGAGGCTGATCTGCTTGTGCATGTGTTGGATTGCACTGCTCCTAATCTTGAGGAGCACCGATCAACGGTGTTACATGTTCTTGAGCAAATCGGCGTATCCAAGGAGAAGCTTGAGAATATGATTGAAGTTTGGAATAAGATTGACAGTGAGGGAGAGGATATGGGAATGGATGTTAATGAATCTCTTGATGATGGTGAAGATGGTGAGGTGAGCAGCCTCTCAGGAGAAGATGATGATGTGGAGTCTGAGCTGTCAACAGGAGCAGCAAACTGTAGGGCTGATGATCCTTTGTCTGAGCTGTCAGCAGAGAATCCCGAAGCCATGGATGACCAAGAACTCGATTCAGAAGATAACCAAGATCATTGGGGTGAAGAAGGTGGTTTCAGCATGGGCCCTAAGACTCTGAATGACCGACAAAGTGAGTACCCTAAGGACTGGGCAGTGGAAAAGCAATTGCAATCACGAGCTCCATCTGGTCCACATGTTAGAACTTCTGCCATAACAGGAGTCGGCTTGCAAGAGTTGTTAGAGCTCATTGATGAGAAACTGAAAGTCCCAAATGTTCTGGAAAAGGGTGTATTTGATCGAAAATGGAGGCCCTCTCGTACAGAGGAGGCGGGTCTAGCAGTTGAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043021 GO:0043022 GO:0044424 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

381

Amino Acids

41.71

Weight (kDa)

4.87

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_M PF16360 33 - 81 3.4e-06 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 93 - 215 5e-16 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 296
AccII CGCG 1 cut(s) 31
AciI CCGC 1 cut(s) 1124
AclWI GGATC 1 cut(s) 785
AcsI RAATTY 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 4 cut(s) 217, 399, 932, 1114
AfiI CCNNNNNNNGG 1 cut(s) 571
AflIII ACRYGT 3 cut(s) 29, 580, 988
AgsI TTSAA 4 cut(s) 149, 155, 632, 1139
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AloI GAACNNNNNNTCC 2 cut(s) 19, 51
AluBI AGCT 9 cut(s) 224, 258, 275, 479, 616, 757, 805, 974, 1039
AluI AGCT 9 cut(s) 224, 258, 275, 479, 616, 757, 805, 974, 1039
Alw21I GWGCWC 3 cut(s) 564, 976, 1041
AlwI GGATC 1 cut(s) 785
Ama87I CYCGRG 2 cut(s) 90, 219
AoxI GGCC 2 cut(s) 898, 1103
ApaI GGGCCC 1 cut(s) 902
ApeKI GCWGC 2 cut(s) 720, 770
ApoI RAATTY 1 cut(s) 165
ArsI GACNNNNNNTTYG 2 cut(s) 19, 51
Asp700I GAANNNNTTC 1 cut(s) 480
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 6 cut(s) 35, 110, 898, 899, 983, 1104
AsuHPI GGTGA 5 cut(s) 334, 713, 722, 727, 887
AvaI CYCGRG 2 cut(s) 90, 219
AvaII GGWCC 3 cut(s) 35, 110, 983
AxyI CCTNAGG 1 cut(s) 936
BaeGI GKGCMC 2 cut(s) 331, 902
BanII GRGCYC 3 cut(s) 902, 976, 1041
BauI CACGAG 1 cut(s) 969
Bbv12I GWGCWC 3 cut(s) 564, 976, 1041
BbvI GCAGC 2 cut(s) 732, 782
BccI CCATC 3 cut(s) 692, 701, 985
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 1 cut(s) 613
BfaI CTAG 2 cut(s) 384, 1130
BfmI CTRYAG 2 cut(s) 349, 778
BfoI RGCGCY 1 cut(s) 90
BfuI GTATCC 1 cut(s) 613
BisI GCNGC 2 cut(s) 721, 771
BlpI GCTNAGC 1 cut(s) 271
BlsI GCNGC 2 cut(s) 722, 772
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 3 cut(s) 35, 110, 983
BmeT110I CYCGRG 2 cut(s) 90, 219
BmgT120I GGNCC 6 cut(s) 35, 110, 898, 899, 983, 1104
BmiI GGNNCC 1 cut(s) 900
BmrFI CCNGG 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 952
BmuI ACTGGG 1 cut(s) 952
BplI GAGNNNNNCTC 2 cut(s) 1093, 1125
Bpu10I CCTNAGC 1 cut(s) 49
Bpu1102I GCTNAGC 1 cut(s) 271
BpuEI CTTGAG 3 cut(s) 575, 608, 638
BsaJI CCNNGG 3 cut(s) 91, 606, 828
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 571
Bse1I ACTGG 2 cut(s) 461, 947
Bse21I CCTNAGG 1 cut(s) 936
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 91, 606, 828
BseGI GGATG 3 cut(s) 211, 682, 838
BseLI CCNNNNNNNGG 1 cut(s) 571
BseMII CTCAG 3 cut(s) 741, 744, 792
BseNI ACTGG 2 cut(s) 461, 947
BseRI GAGGAG 3 cut(s) 193, 572, 1133
BseSI GKGCMC 2 cut(s) 331, 902
BseXI GCAGC 2 cut(s) 732, 782
BseYI CCCAGC 1 cut(s) 305
BsgI GTGCAG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 31
BshFI GGCC 2 cut(s) 900, 1105
BsiHKAI GWGCWC 3 cut(s) 564, 976, 1041
BsiHKCI CYCGRG 2 cut(s) 90, 219
BslFI GGGAC 1 cut(s) 1046
BslI CCNNNNNNNGG 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 1046
BsnI GGCC 2 cut(s) 900, 1105
BsoBI CYCGRG 2 cut(s) 90, 219
Bsp120I GGGCCC 1 cut(s) 898
Bsp1286I GDGCHC 5 cut(s) 331, 564, 902, 976, 1041
Bsp143I GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
Bsp1720I GCTNAGC 1 cut(s) 271
Bsp19I CCATGG 1 cut(s) 828
BspACI CCGC 1 cut(s) 1124
BspANI GGCC 2 cut(s) 900, 1105
BspCNI CTCAG 3 cut(s) 740, 745, 793
BspFNI CGCG 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 900
BspPI GGATC 1 cut(s) 785
BsrI ACTGG 2 cut(s) 461, 947
BssECI CCNNGG 3 cut(s) 91, 606, 828
BssMI GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
BssNAI GTATAC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 969
BssT1I CCWWGG 2 cut(s) 606, 828
Bst1107I GTATAC 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 969
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 7 cut(s) 280, 353, 376, 439, 574, 653, 779
BstC8I GCNNGC 1 cut(s) 1022
BstDEI CTNAG 7 cut(s) 49, 271, 727, 753, 801, 903, 936
BstDSI CCRYGG 1 cut(s) 828
BstF5I GGATG 3 cut(s) 211, 682, 838
BstFNI CGCG 1 cut(s) 31
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 6 cut(s) 346, 517, 569, 793, 868, 1093
BstMBI GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
BstMWI GCNNNNNNNGC 3 cut(s) 281, 485, 1130
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 3 cut(s) 530, 584, 992
BstSCI CCNGG 1 cut(s) 112
BstSFI CTRYAG 2 cut(s) 349, 778
BstSLI GKGCMC 2 cut(s) 331, 902
BstUI CGCG 1 cut(s) 31
BstV1I GCAGC 2 cut(s) 732, 782
BstZ17I GTATAC 1 cut(s) 297
Bsu36I CCTNAGG 1 cut(s) 936
BsuI GTATCC 1 cut(s) 613
BsuRI GGCC 2 cut(s) 900, 1105
BtgI CCRYGG 1 cut(s) 828
BtsCI GGATG 3 cut(s) 211, 682, 838
BtsI GCAGTG 2 cut(s) 540, 954
BtsIMutI CAGTG 5 cut(s) 358, 468, 540, 658, 954
Cac8I GCNNGC 1 cut(s) 1022
CfoI GCGC 1 cut(s) 89
Cfr13I GGNCC 6 cut(s) 35, 110, 898, 899, 983, 1104
CseI GACGC 1 cut(s) 54
Csp6I GTAC 4 cut(s) 216, 398, 931, 1113
CviAII CATG 7 cut(s) 251, 485, 527, 581, 829, 895, 989
CviQI GTAC 4 cut(s) 216, 398, 931, 1113
DdeI CTNAG 7 cut(s) 49, 271, 727, 753, 801, 903, 936
DpnI GATC 6 cut(s) 345, 516, 568, 792, 867, 1092
DpnII GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
DraIII CACNNNGTG 1 cut(s) 341
Ecl136II GAGCTC 2 cut(s) 974, 1039
Eco130I CCWWGG 2 cut(s) 606, 828
Eco24I GRGCYC 3 cut(s) 902, 976, 1041
Eco47I GGWCC 3 cut(s) 35, 110, 983
Eco53kI GAGCTC 2 cut(s) 974, 1039
Eco57I CTGAAG 1 cut(s) 393
Eco81I CCTNAGG 1 cut(s) 936
Eco88I CYCGRG 2 cut(s) 90, 219
EcoICRI GAGCTC 2 cut(s) 974, 1039
EcoO109I RGGNCCY 2 cut(s) 899, 1104
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 606, 828
EcoT38I GRGCYC 3 cut(s) 902, 976, 1041
ErhI CCWWGG 2 cut(s) 606, 828
FaeI CATG 7 cut(s) 254, 488, 530, 584, 832, 898, 992
FaqI GGGAC 1 cut(s) 1046
FatI CATG 7 cut(s) 250, 484, 526, 580, 828, 894, 988
FauI CCCGC 1 cut(s) 1117
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 2 cut(s) 721, 771
FokI GGATG 3 cut(s) 218, 689, 845
FriOI GRGCYC 3 cut(s) 902, 976, 1041
Fsp4HI GCNGC 2 cut(s) 721, 771
FspBI CTAG 2 cut(s) 384, 1130
GlaI GCGC 1 cut(s) 88
GluI GCNGC 2 cut(s) 721, 771
GsaI CCCAGC 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 2 cut(s) 900, 1105
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 89
Hin1II CATG 7 cut(s) 254, 488, 530, 584, 832, 898, 992
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HincII GTYRAC 2 cut(s) 379, 762
HindII GTYRAC 2 cut(s) 379, 762
HindIII AAGCTT 2 cut(s) 477, 614
HinfI GANTC 8 cut(s) 117, 356, 686, 749, 817, 848, 907, 1014
HphI GGTGA 5 cut(s) 334, 713, 722, 727, 887
Hpy166II GTNNAC 6 cut(s) 44, 297, 379, 398, 762, 986
Hpy188I TCNGA 5 cut(s) 454, 754, 802, 853, 912
Hpy188III TCNNGA 8 cut(s) 360, 554, 587, 692, 729, 821, 969, 1073
Hpy8I GTNNAC 6 cut(s) 44, 297, 379, 398, 762, 986
Hpy99I CGWCG 2 cut(s) 162, 216
HpyAV CCTTC 2 cut(s) 417, 875
HpyCH4III ACNGT 7 cut(s) 280, 353, 376, 439, 574, 653, 779
HpyCH4IV ACGT 1 cut(s) 232
HpyCH4V TGCA 7 cut(s) 229, 371, 466, 526, 540, 964, 1024
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 485, 1130
HpyF3I CTNAG 7 cut(s) 49, 271, 727, 753, 801, 903, 936
HpySE526I ACGT 1 cut(s) 232
Hsp92II CATG 7 cut(s) 254, 488, 530, 584, 832, 898, 992
HspAI GCGC 1 cut(s) 87
Kzo9I GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
LmnI GCTCC 4 cut(s) 550, 559, 767, 979
Lsp1109I GCAGC 2 cut(s) 732, 782
MaeI CTAG 2 cut(s) 384, 1130
MaeII ACGT 1 cut(s) 232
MaeIII GTNAC 1 cut(s) 576
MalI GATC 6 cut(s) 345, 516, 568, 792, 867, 1092
MboI GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
MboII GAAGA 7 cut(s) 174, 395, 509, 716, 746, 866, 890
MfeI CAATTG 1 cut(s) 959
MhlI GDGCHC 5 cut(s) 331, 564, 902, 976, 1041
MluCI AATT 3 cut(s) 165, 198, 959
MluI ACGCGT 1 cut(s) 29
MlyI GAGTC 3 cut(s) 758, 901, 1023
MmeI TCCRAC 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 2 cut(s) 201, 681
MslI CAYNNNNRTG 1 cut(s) 873
MspR9I CCNGG 1 cut(s) 114
MunI CAATTG 1 cut(s) 959
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 31
MwoI GCNNNNNNNGC 3 cut(s) 281, 485, 1130
NcoI CCATGG 1 cut(s) 828
NdeII GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
NlaIII CATG 7 cut(s) 254, 488, 530, 584, 832, 898, 992
NlaIV GGNNCC 1 cut(s) 900
NspI RCATGY 3 cut(s) 530, 584, 992
PaeR7I CTCGAG 1 cut(s) 219
PciI ACATGT 2 cut(s) 580, 988
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 5 cut(s) 117, 356, 686, 817, 848
PkrI GCNGC 2 cut(s) 722, 772
PleI GAGTC 3 cut(s) 757, 901, 1022
PpsI GAGTC 3 cut(s) 757, 901, 1022
PscI ACATGT 2 cut(s) 580, 988
Psp124BI GAGCTC 2 cut(s) 976, 1041
Psp6I CCWGG 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 900
PspOMI GGGCCC 1 cut(s) 898
PspPI GGNCC 6 cut(s) 35, 110, 898, 899, 983, 1104
PspXI VCTCGAGB 1 cut(s) 219
RsaI GTAC 4 cut(s) 217, 399, 932, 1114
RsaNI GTAC 4 cut(s) 216, 398, 931, 1113
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 2 cut(s) 976, 1041
SaqAI TTAA 2 cut(s) 201, 681
SatI GCNGC 2 cut(s) 721, 771
Sau3AI GATC 6 cut(s) 343, 514, 566, 790, 865, 1090
Sau96I GGNCC 6 cut(s) 35, 110, 898, 899, 983, 1104
SchI GAGTC 3 cut(s) 758, 901, 1023
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 5 cut(s) 331, 564, 902, 976, 1041
SfcI CTRYAG 2 cut(s) 349, 778
Sfr274I CTCGAG 1 cut(s) 219
SinI GGWCC 3 cut(s) 35, 110, 983
SlaI CTCGAG 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 4 cut(s) 219, 554, 587, 617
SmoI CTYRAG 4 cut(s) 219, 554, 587, 617
Sse9I AATT 3 cut(s) 165, 198, 959
SsiI CCGC 1 cut(s) 1124
SspMI CTAG 2 cut(s) 384, 1130
SstI GAGCTC 2 cut(s) 976, 1041
StyD4I CCNGG 1 cut(s) 112
StyI CCWWGG 2 cut(s) 606, 828
TaaI ACNGT 7 cut(s) 280, 353, 376, 439, 574, 653, 779
TaiI ACGT 1 cut(s) 235
TaqI TCGA 4 cut(s) 220, 361, 846, 1093
TaqII GACCGA 1 cut(s) 933
TasI AATT 3 cut(s) 165, 198, 959
TatI WGTACW 1 cut(s) 397
TfiI GAWTC 5 cut(s) 117, 356, 686, 817, 848
Tru1I TTAA 2 cut(s) 201, 681
Tru9I TTAA 2 cut(s) 201, 681
TscAI CASTG 5 cut(s) 358, 468, 547, 658, 954
TseI GCWGC 2 cut(s) 720, 770
TspDTI ATGAA 3 cut(s) 109, 473, 699
TspRI CASTG 5 cut(s) 358, 468, 547, 658, 954
VpaK11BI GGWCC 3 cut(s) 35, 110, 983
XapI RAATTY 1 cut(s) 165
XceI RCATGY 3 cut(s) 530, 584, 992
XhoI CTCGAG 1 cut(s) 219
XmiI GTMKAC 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 2 cut(s) 384, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.