MD02G1197300.v1.1

Acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007995
Physical Location & Seq
Forward (+)
19058804 .. 19067263
8460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1197300.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGCAGACCACGGTATCACCTCCCCCCTCCTCTCTCCTCCACCTTCCGATCACTCCCACTTGATCCTCACCGTCCAAGACGACACCGATACCGATAGCCACCACAACGGCACCCACACTCATCAGAGCACCAGCGCTCACCACCACCACTCTCGTAACCCTTTTGCGTTTCTCGGGTCCGATGGGTTCACCGTGCCCGTTTCCACCACCGCGGACCCGTTTCGGAACCACACCCTGGAGATTACGGGTGTTTATGAGTGGTTAAAGATCGGAATTTGTCTGCCCGTCGCCCTGGCTCGGCTGGTGCTGTTTGGGGCCTCGTTGGTGATTGGGTTTTTGGCGACGAAATTGGCTCTTCAGGGGTGGAAGGATAAGAAGAACCCTATGCCTAGGTGGAGGTGCAGGATTATGTGGATCACTAGGGTCTGTACTCGATGCATTCTCTTCGCTTTTGGCTACCACTGGATAAGACGGAAAGGAAAACCAGCTCCTAGAGACATTGCTCCAATAGTCGTTTCAAACCATGTATCTTTTATTGAACCTATCTTCTATTTCTATGAATTATTCCCCACAATTGTGGCATCGGAATCCCATGATTCCCTACCTTTTGTAGGGACGATCATCAGAGCAATGCAGGTGATATATGTTAATAGGTTTTCAGCATCATCAAGGAAGCAAGCCGTTAGTGAAATCAAGAGAAAAGCTTCGTGTGGTAGATTTCCTCAAGTTCTTTTATTTCCCGAGGGAACCACAACCAACGGGAGATATCTCATTTCATTTGAACTTGGTGCATTCATCCCTGGTTTTGCCATCCAACCAGTAGTTGTACGATATCCCCATGTACACTTTGACCAATCCTGGGGGCACATTTCATTGGCAAGGCTCATGTTTAGAATGTTCACACAGTTTCACAATTTCATGGAGGTAGAGTACCTTCCTGTTGTTTCACCCCTTGAGAACAAGAAAGAAAGTGCTATCTGTTTTTCGGAGAGGACTAGTCATGCTATTGCAACTGCACTCAACGTTGTACAGACATCTCATTCATATGGAGACTTAATGCTTCTCACGAAAGCATCTCAGTCAAAATTGAAACCGGAGCGACCTTCGGTGTATATGGTTGAAATGGCAAGTGTAAAATCATTACTCCATATAAGTGGCATGGAAGCTGTAGACTTTCTGGATAAGTTTCTTTCTATGAATCCAGACCCAAGAGGTCATGTTCACTACCATGATTTCTTAAGGGTGCTAAGACTCAAGACTTGTAGCTTTTCCGAAGAGATATTTGCATTTATGGATGTGGAGAAAAGTGGAGTAATAACGTTTAAGCAGTTCTTATTTGGATCAGCGCATGTCGTGAAGCAGCCGTTGTTCCGTAGAGCCTGTGAATTCGCCTTTTCAGAATGCGTTTCTGGGGAGAGTGACCACGTTTCAGAACAAAAATTTGGAGAGTCCATCAGGCCCGCAATCCCGGATTTGAATGAGGATGAGGTCCGTGAACTGTTCAATCTATTTGATTCTGATAATGACGGAAGAATCAGCAAGGAAGATTTTTTGACGTGCCTAAGAAAAAACCCACTACTGATTGCGCTTTTCTCGCCTTGTTTGCTTAACAAAGACATTTCAGAAGATAGTAATAGGTTGGTGGAGGAGATTGTGTAG

Protein Analysis

553

Amino Acids

62.59

Weight (kDa)

7.15

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 164 - 276 1.6e-14 Acyltransferase
EF-hand_7 PF13499 461 - 519 2e-07 EF-hand domain pair
EF-hand_8 PF13833 490 - 523 7.4e-09 EF-hand domain pair
EF-hand_1 PF00036 496 - 523 3.4e-07 EF hand domain
EF-hand_6 PF13405 496 - 523 5e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 625
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 235
AccI GTMKAC 1 cut(s) 1170
AccII CGCG 1 cut(s) 212
AciI CCGC 3 cut(s) 210, 212, 1461
AclI AACGTT 2 cut(s) 1023, 1319
AclWI GGATC 3 cut(s) 58, 422, 1348
AcsI RAATTY 3 cut(s) 273, 1385, 1439
AcuI CTGAAG 1 cut(s) 341
AfaI GTAC 5 cut(s) 430, 828, 843, 932, 1029
AfeI AGCGCT 1 cut(s) 136
AfiI CCNNNNNNNGG 4 cut(s) 235, 297, 611, 859
AflII CTTAAG 1 cut(s) 1237
AgsI TTSAA 7 cut(s) 519, 539, 782, 1090, 1121, 1477, 1504
AhlI ACTAGT 1 cut(s) 995
AjiI CACGTC 1 cut(s) 1557
AjnI CCWGG 4 cut(s) 234, 291, 799, 857
AjuI GAANNNNNNNTTGG 4 cut(s) 499, 531, 1425, 1457
AleI CACNNNNGTG 1 cut(s) 575
AluBI AGCT 4 cut(s) 488, 704, 1166, 1266
AluI AGCT 4 cut(s) 488, 704, 1166, 1266
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 2 cut(s) 489, 1044
AlwI GGATC 3 cut(s) 58, 422, 1348
Ama87I CYCGRG 2 cut(s) 173, 740
Aor51HI AGCGCT 1 cut(s) 136
AoxI GGCC 2 cut(s) 315, 1457
ApeKI GCWGC 1 cut(s) 1360
ApoI RAATTY 3 cut(s) 273, 1385, 1439
Asp700I GAANNNNTTC 1 cut(s) 703
AspA2I CCTAGG 1 cut(s) 389
AspLEI GCGC 3 cut(s) 137, 1348, 1588
AspS9I GGNCC 5 cut(s) 177, 214, 315, 1458, 1489
AsuC2I CCSGG 1 cut(s) 1469
AsuHPI GGTGA 7 cut(s) 10, 61, 131, 181, 337, 649, 939
AvaI CYCGRG 2 cut(s) 173, 740
AvaII GGWCC 3 cut(s) 177, 214, 1489
AvrII CCTAGG 1 cut(s) 389
BaeGI GKGCMC 2 cut(s) 198, 867
BaeI ACNNNNGTAYC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 918, 950
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 1372
BccI CCATC 3 cut(s) 176, 818, 1460
BceAI ACGGC 3 cut(s) 124, 665, 1348
BcgI CGANNNNNNTGC 2 cut(s) 427, 461
BciT130I CCWGG 4 cut(s) 236, 293, 801, 859
BcnI CCSGG 1 cut(s) 1469
BcoDI GTCTC 2 cut(s) 489, 1044
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 4 cut(s) 390, 420, 492, 996
BfmI CTRYAG 1 cut(s) 1167
BfoI RGCGCY 1 cut(s) 138
BfrI CTTAAG 1 cut(s) 1237
BfuAI ACCTGC 1 cut(s) 625
BisI GCNGC 1 cut(s) 1361
BlnI CCTAGG 1 cut(s) 389
BlsI GCNGC 1 cut(s) 1362
Bme1390I CCNGG 5 cut(s) 236, 293, 801, 859, 1469
Bme18I GGWCC 3 cut(s) 177, 214, 1489
BmeT110I CYCGRG 2 cut(s) 173, 740
BmgBI CACGTC 1 cut(s) 1557
BmgT120I GGNCC 5 cut(s) 177, 214, 315, 1458, 1489
BmiI GGNNCC 6 cut(s) 112, 178, 216, 227, 316, 748
BmrFI CCNGG 5 cut(s) 236, 293, 801, 859, 1469
BmsI GCATC 4 cut(s) 425, 590, 671, 1082
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 3 cut(s) 708, 974, 1238
BpuMI CCSGG 1 cut(s) 1469
BsaJI CCNNGG 8 cut(s) 10, 210, 234, 291, 389, 741, 799, 858
BsaWI WCCGGW 1 cut(s) 1093
BsaXI ACNNNNNCTCC 3 cut(s) 914, 944, 1640
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 297, 611, 859
Bse1I ACTGG 2 cut(s) 467, 818
Bse3DI GCAATG 2 cut(s) 498, 636
BseBI CCWGG 4 cut(s) 236, 293, 801, 859
BseDI CCNNGG 8 cut(s) 10, 210, 234, 291, 389, 741, 799, 858
BseGI GGATG 4 cut(s) 795, 810, 1300, 1489
BseLI CCNNNNNNNGG 4 cut(s) 235, 297, 611, 859
BseMI GCAATG 2 cut(s) 498, 636
BseMII CTCAG 1 cut(s) 1091
BseNI ACTGG 2 cut(s) 467, 818
BseRI GAGGAG 2 cut(s) 20, 27
BseSI GKGCMC 2 cut(s) 198, 867
BseXI GCAGC 1 cut(s) 1372
BsgI GTGCAG 2 cut(s) 421, 999
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 2 cut(s) 317, 1459
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 131
BsiHKCI CYCGRG 2 cut(s) 173, 740
BsiSI CCGG 2 cut(s) 1094, 1469
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 4 cut(s) 235, 297, 611, 859
BsmAI GTCTC 2 cut(s) 489, 1044
BsmFI GGGAC 1 cut(s) 628
BsmI GAATGC 3 cut(s) 438, 791, 1406
BsnI GGCC 2 cut(s) 317, 1459
BsoBI CYCGRG 2 cut(s) 173, 740
Bsp1286I GDGCHC 3 cut(s) 131, 198, 867
Bsp1407I TGTACA 2 cut(s) 841, 1027
Bsp143I GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
BspACI CCGC 3 cut(s) 210, 212, 1461
BspANI GGCC 2 cut(s) 317, 1459
BspCNI CTCAG 1 cut(s) 1090
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 6 cut(s) 112, 178, 216, 227, 316, 748
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 3 cut(s) 58, 422, 1348
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 1237
BsrDI GCAATG 2 cut(s) 498, 636
BsrGI TGTACA 2 cut(s) 841, 1027
BsrI ACTGG 2 cut(s) 467, 818
BssECI CCNNGG 8 cut(s) 10, 210, 234, 291, 389, 741, 799, 858
BssMI GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
BssT1I CCWWGG 1 cut(s) 389
Bst2UI CCWGG 4 cut(s) 236, 293, 801, 859
Bst4CI ACNGT 5 cut(s) 14, 73, 193, 906, 1500
Bst6I CTCTTC 3 cut(s) 360, 449, 1269
BstAFI CTTAAG 1 cut(s) 1237
BstAUI TGTACA 2 cut(s) 841, 1027
BstC8I GCNNGC 2 cut(s) 678, 1461
BstDEI CTNAG 3 cut(s) 1077, 1247, 1562
BstDSI CCRYGG 2 cut(s) 10, 210
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 4 cut(s) 795, 810, 1300, 1489
BstFNI CGCG 1 cut(s) 212
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 3 cut(s) 137, 1348, 1588
BstKTI GATC 6 cut(s) 52, 66, 270, 417, 621, 1343
BstMAI GTCTC 2 cut(s) 489, 1044
BstMBI GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
BstMWI GCNNNNNNNGC 2 cut(s) 1594, 1603
BstNI CCWGG 4 cut(s) 236, 293, 801, 859
BstNSI RCATGY 1 cut(s) 1352
BstSCI CCNGG 5 cut(s) 234, 291, 799, 857, 1467
BstSFI CTRYAG 1 cut(s) 1167
BstSLI GKGCMC 2 cut(s) 198, 867
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 1372
BstXI CCANNNNNNTGG 2 cut(s) 577, 1154
BsuRI GGCC 2 cut(s) 317, 1459
BtgI CCRYGG 2 cut(s) 10, 210
BtrI CACGTC 1 cut(s) 1557
BtsCI GGATG 4 cut(s) 795, 810, 1300, 1489
BtsIMutI CAGTG 1 cut(s) 460
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 2 cut(s) 678, 1461
CfoI GCGC 3 cut(s) 137, 1348, 1588
Cfr13I GGNCC 5 cut(s) 177, 214, 315, 1458, 1489
Cfr42I CCGCGG 1 cut(s) 213
Csp6I GTAC 5 cut(s) 429, 827, 842, 931, 1028
CviQI GTAC 5 cut(s) 429, 827, 842, 931, 1028
DdeI CTNAG 3 cut(s) 1077, 1247, 1562
DpnI GATC 6 cut(s) 51, 65, 269, 416, 620, 1342
DpnII GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
Eam1104I CTCTTC 3 cut(s) 360, 449, 1269
EarI CTCTTC 3 cut(s) 360, 449, 1269
Eco130I CCWWGG 1 cut(s) 389
Eco32I GATATC 2 cut(s) 767, 833
Eco47I GGWCC 3 cut(s) 177, 214, 1489
Eco47III AGCGCT 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 341
Eco88I CYCGRG 2 cut(s) 173, 740
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 315
EcoRI GAATTC 1 cut(s) 1385
EcoRII CCWGG 4 cut(s) 234, 291, 799, 857
EcoRV GATATC 2 cut(s) 767, 833
EcoT14I CCWWGG 1 cut(s) 389
EcoT22I ATGCAT 1 cut(s) 440
ErhI CCWWGG 1 cut(s) 389
FalI AAGNNNNNCTT 2 cut(s) 1316, 1348
FaqI GGGAC 1 cut(s) 628
FauI CCCGC 1 cut(s) 1468
FauNDI CATATG 1 cut(s) 1045
FblI GTMKAC 1 cut(s) 1170
Fnu4HI GCNGC 1 cut(s) 1361
FokI GGATG 4 cut(s) 782, 797, 1307, 1496
Fsp4HI GCNGC 1 cut(s) 1361
FspBI CTAG 4 cut(s) 390, 420, 492, 996
GlaI GCGC 3 cut(s) 136, 1347, 1587
GluI GCNGC 1 cut(s) 1361
GsuI CTGGAG 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 2 cut(s) 317, 1459
HapII CCGG 2 cut(s) 1094, 1469
HhaI GCGC 3 cut(s) 137, 1348, 1588
Hin6I GCGC 3 cut(s) 135, 1346, 1586
HinP1I GCGC 3 cut(s) 135, 1346, 1586
HindIII AAGCTT 1 cut(s) 702
HinfI GANTC 7 cut(s) 587, 596, 1198, 1251, 1448, 1514, 1533
HpaII CCGG 2 cut(s) 1094, 1469
HphI GGTGA 7 cut(s) 10, 61, 131, 181, 337, 649, 939
Hpy166II GTNNAC 6 cut(s) 189, 844, 900, 1171, 1222, 1496
Hpy188III TCNNGA 7 cut(s) 694, 740, 1066, 1178, 1202, 1255, 1354
Hpy8I GTNNAC 6 cut(s) 189, 844, 900, 1171, 1222, 1496
Hpy99I CGWCG 2 cut(s) 290, 346
HpyAV CCTTC 4 cut(s) 54, 361, 944, 1113
HpyCH4III ACNGT 5 cut(s) 14, 73, 193, 906, 1500
HpyCH4IV ACGT 4 cut(s) 1023, 1319, 1425, 1556
HpyCH4V TGCA 7 cut(s) 402, 438, 634, 791, 1010, 1016, 1286
HpyF10VI GCNNNNNNNGC 2 cut(s) 1594, 1603
HpyF3I CTNAG 3 cut(s) 1077, 1247, 1562
HpySE526I ACGT 4 cut(s) 1023, 1319, 1425, 1556
HspAI GCGC 3 cut(s) 135, 1346, 1586
KspI CCGCGG 1 cut(s) 213
Kzo9I GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 3 cut(s) 493, 508, 1096
Lsp1109I GCAGC 1 cut(s) 1372
LweI GCATC 4 cut(s) 425, 590, 671, 1082
MaeI CTAG 4 cut(s) 390, 420, 492, 996
MaeII ACGT 4 cut(s) 1023, 1319, 1425, 1556
MaeIII GTNAC 2 cut(s) 155, 1418
MalI GATC 6 cut(s) 51, 65, 269, 416, 620, 1342
MboI GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
MboII GAAGA 8 cut(s) 347, 388, 436, 538, 1286, 1542, 1556, 1637
MfeI CAATTG 1 cut(s) 573
MhlI GDGCHC 3 cut(s) 131, 198, 867
MluCI AATT 8 cut(s) 273, 347, 560, 573, 913, 1085, 1385, 1439
MlyI GAGTC 2 cut(s) 1245, 1457
MmeI TCCRAC 1 cut(s) 838
Mph1103I ATGCAT 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 703
MseI TTAA 6 cut(s) 263, 648, 1055, 1238, 1323, 1608
MslI CAYNNNNRTG 4 cut(s) 575, 1044, 1152, 1227
MspA1I CMGCKG 1 cut(s) 212
MspCI CTTAAG 1 cut(s) 1237
MspI CCGG 2 cut(s) 1094, 1469
MspR9I CCNGG 5 cut(s) 236, 293, 801, 859, 1469
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 3 cut(s) 438, 791, 1406
MvaI CCWGG 4 cut(s) 236, 293, 801, 859
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 2 cut(s) 1594, 1603
NciI CCSGG 1 cut(s) 1469
NdeI CATATG 1 cut(s) 1045
NdeII GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
NlaIV GGNNCC 6 cut(s) 112, 178, 216, 227, 316, 748
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 1418
NsiI ATGCAT 1 cut(s) 440
NspI RCATGY 1 cut(s) 1352
OliI CACNNNNGTG 1 cut(s) 575
PaqCI CACCTGC 1 cut(s) 625
PciSI GCTCTTC 1 cut(s) 360
PctI GAATGC 3 cut(s) 438, 791, 1406
PdmI GAANNNNTTC 1 cut(s) 703
PfeI GAWTC 5 cut(s) 587, 596, 1198, 1514, 1533
PflMI CCANNNNNTGG 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 1467
PkrI GCNGC 1 cut(s) 1362
PleI GAGTC 2 cut(s) 1245, 1456
PpsI GAGTC 2 cut(s) 1245, 1456
Psp1406I AACGTT 2 cut(s) 1023, 1319
Psp6I CCWGG 4 cut(s) 234, 291, 799, 857
PspGI CCWGG 4 cut(s) 234, 291, 799, 857
PspN4I GGNNCC 6 cut(s) 112, 178, 216, 227, 316, 748
PspPI GGNCC 5 cut(s) 177, 214, 315, 1458, 1489
RsaI GTAC 5 cut(s) 430, 828, 843, 932, 1029
RsaNI GTAC 5 cut(s) 429, 827, 842, 931, 1028
RseI CAYNNNNRTG 4 cut(s) 575, 1044, 1152, 1227
SacII CCGCGG 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 6 cut(s) 263, 648, 1055, 1238, 1323, 1608
SatI GCNGC 1 cut(s) 1361
Sau3AI GATC 6 cut(s) 49, 63, 267, 414, 618, 1340
Sau96I GGNCC 5 cut(s) 177, 214, 315, 1458, 1489
SchI GAGTC 2 cut(s) 1245, 1457
ScrFI CCNGG 5 cut(s) 236, 293, 801, 859, 1469
SduI GDGCHC 3 cut(s) 131, 198, 867
SfaNI GCATC 4 cut(s) 425, 590, 671, 1082
SfcI CTRYAG 1 cut(s) 1167
Sfr303I CCGCGG 1 cut(s) 213
SgrBI CCGCGG 1 cut(s) 213
SinI GGWCC 3 cut(s) 177, 214, 1489
SmiMI CAYNNNNRTG 4 cut(s) 575, 1044, 1152, 1227
SmlI CTYRAG 4 cut(s) 723, 953, 1237, 1253
SmoI CTYRAG 4 cut(s) 723, 953, 1237, 1253
SpeI ACTAGT 1 cut(s) 995
Sse9I AATT 8 cut(s) 273, 347, 560, 573, 913, 1085, 1385, 1439
SsiI CCGC 3 cut(s) 210, 212, 1461
SspMI CTAG 4 cut(s) 390, 420, 492, 996
StyD4I CCNGG 5 cut(s) 234, 291, 799, 857, 1467
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 5 cut(s) 14, 73, 193, 906, 1500
TaiI ACGT 4 cut(s) 1026, 1322, 1428, 1559
TaqI TCGA 1 cut(s) 433
TasI AATT 8 cut(s) 273, 347, 560, 573, 913, 1085, 1385, 1439
TatI WGTACW 3 cut(s) 428, 841, 1027
TfiI GAWTC 5 cut(s) 587, 596, 1198, 1514, 1533
Tru1I TTAA 6 cut(s) 263, 648, 1055, 1238, 1323, 1608
Tru9I TTAA 6 cut(s) 263, 648, 1055, 1238, 1323, 1608
TscAI CASTG 1 cut(s) 467
TseFI GTSAC 1 cut(s) 1418
TseI GCWGC 1 cut(s) 1360
Tsp45I GTSAC 1 cut(s) 1418
TspDTI ATGAA 7 cut(s) 573, 765, 784, 861, 907, 1032, 1211
TspGWI ACGGA 4 cut(s) 487, 1361, 1481, 1542
TspRI CASTG 1 cut(s) 467
Van91I CCANNNNNTGG 1 cut(s) 235
Vha464I CTTAAG 1 cut(s) 1237
VpaK11BI GGWCC 3 cut(s) 177, 214, 1489
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 3 cut(s) 273, 1385, 1439
XceI RCATGY 1 cut(s) 1352
XmaJI CCTAGG 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 1170
XmnI GAANNNNTTC 1 cut(s) 703
XspI CTAG 4 cut(s) 390, 420, 492, 996
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.