MD07G1123700.v1.1

Acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007995
Physical Location & Seq
Reverse (-)
17189884 .. 17196390
6507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1123700.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGCCTTCAATAGCGTTGTACGAACCCATTGTAAATATTTGTTTCTCTCTCTCTTACTTACAGAAGCTAGCGTGTAGCTTCATTAGCCTTACTGCCACTCTGCTCCCCCAAGAAAAAACACCCACTTTAATGGCAGACCAAGATCTCACCTCCCCCTTCCTCTCTCCTCCACCGTCCGATCACCCCCACTTGATCCTCACCATCCAAGACGACACCGATACCGAATCCCACCACAACCACAACGGCATCCACACCCATCAGAGCACCAGCGCCGACCACCACCACCACTTTCGCAACCCATTTGCATTTCTCGGGTCCGATGGGTTGACCGTGCCCGTTTCCATCACCGCCGACCCTTTTCGGAACCACACCCTCGAGATTACGGGTATTTACGAGTGGTTAAAGATCGGAATTTGCCTGCCCATCGCGCTTGTTCGGCTGGTGCTGTTTGGGGTGTCTTTGCTGATTGGGTTTTTGGCGACGAAATTGGCTCTTCAGGGGTGGAAGGATAAGAAGAGCCCTATGCCGAGGTGGAGGTGCAGGATTATGTGGATCACTAGGGTCTGTACTCGATGCATTCTCTTCGCTTTTGGCTACCATTGGATAAGACATAAAGGAAAACCTGCTCCCCGAGACATTGCTCCAATAGTTGTTTCTAACCATGTGTCTTTTATTGAACCTATCTTCTTTTTCTATGAATTATTCCCTACAATTGTGGCATCCGAATCCCATGATTCCCTACCTTTTGTTGGGACGATCATCAGAGCAATGCAGGTGATATATGTTAACAGGTTTTCAGCATCATCAAGGAAGCATGCTGTCAGTGAAATCAAGAGAAAAGCTTCATGTGGTAGATTTCCTCGAGTTCTTTTATTTCCCGAGGGAACCACAACCAATGGGAGATATCTCATTTCATTTGAACTTGGTGCATTCATCCCTGGTTTCACCATCCAACCAGTAGTTGTACGATATCCCCACGTACACTTTGATCAATCCTGGGGGCACATTTCTTTGGCAAAGCTCATGTTTAGAATGTTCACACAGTTTCACAATTTCATGGAGGTAGAGTACCTTTCTGTTGTTTCACTCTTTGATAACAAGAAAGAAAGTGCTACCCGTTTTTCGGAGAGGACTAGTCATGCTATTGCAACTGCACTCAACGTTGTACAGACATCTCATTCATATGGAGATTTAATGCTTCTCATGAAAGCATCTCAGTCAAAATTGAAACTGGAGCAACCTTCAGTGTATATGGTTGAAATGGCAAGTGTAAAGTCATTACTCCATATAAGCAGCATAGAGGCTGTGGACTTTCTAGATAAGTTTCTTTCTATGAATCCAGACCCAAGAGGTCATGTTCACTACCATGATTTCCTAAGGGTGCTAAGACTCAAGGATTGTAGCTATTCCGAAGAGATATTTGCATTCATGGATGTTGAGAGAAATGGAGTAATAACGTTTAAGCAGTTCTTATTTGGATCAGCGCATGTCGTTAAGCGGCCATTGTTCCGCAGAGCCTGTGAATTAGCCTTTTCTGAATGTGTTTCTGGGGAGAATGACTACGTTTCAGAACAAAAATTTGGAGAGTCCATCGGACATGCAATCCCGGATTTGAATGAGGATGAGGTCCGTGGACTGTTCAATCTATTTGATTCTGATAATGATGGAAGAATCAGCAGGGAAGATTTTTTGACCTGCCTAAGAAAAAACCCACTACTGATTGCAGTTTTCTCGCCTTGTTTGCTTAACAAAGACATTTCAGAAGATGGAAATAGGTTGGTGGAGGAGATTGTGTAG

Protein Analysis

599

Amino Acids

68.03

Weight (kDa)

6.86

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 208 - 322 2.4e-15 Acyltransferase
EF-hand_7 PF13499 509 - 565 8.7e-07 EF-hand domain pair
EF-hand_8 PF13833 537 - 569 1.3e-07 EF-hand domain pair
EF-hand_1 PF00036 542 - 569 5.3e-06 EF hand domain
EF-hand_6 PF13405 543 - 569 4.2e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 763
Acc36I ACCTGC 3 cut(s) 631, 763, 1703
AccII CGCG 1 cut(s) 428
AciI CCGC 3 cut(s) 348, 1498, 1510
AclI AACGTT 2 cut(s) 1161, 1457
AclWI GGATC 3 cut(s) 187, 560, 1486
AcoI YGGCCR 1 cut(s) 1499
AcsI RAATTY 2 cut(s) 411, 1577
AcuI CTGAAG 2 cut(s) 479, 1227
AfaI GTAC 6 cut(s) 20, 568, 966, 981, 1070, 1167
AfiI CCNNNNNNNGG 2 cut(s) 749, 1123
AgsI TTSAA 7 cut(s) 9, 677, 920, 1228, 1259, 1615, 1642
AhlI ACTAGT 1 cut(s) 1133
AjnI CCWGG 2 cut(s) 937, 995
AjuI GAANNNNNNNTTGG 4 cut(s) 637, 669, 1563, 1595
AluBI AGCT 5 cut(s) 67, 78, 842, 1021, 1404
AluI AGCT 5 cut(s) 67, 78, 842, 1021, 1404
Alw21I GWGCWC 1 cut(s) 266
Alw26I GTCTC 1 cut(s) 627
AlwI GGATC 3 cut(s) 187, 560, 1486
AlwNI CAGNNNCTG 1 cut(s) 1518
Ama87I CYCGRG 5 cut(s) 311, 374, 630, 861, 878
AoxI GGCC 1 cut(s) 1499
ApeKI GCWGC 1 cut(s) 1293
ApoI RAATTY 2 cut(s) 411, 1577
Asp700I GAANNNNTTC 1 cut(s) 841
AspLEI GCGC 3 cut(s) 272, 430, 1486
AspS9I GGNCC 2 cut(s) 315, 1627
AsuC2I CCSGG 1 cut(s) 1607
AsuHPI GGTGA 6 cut(s) 139, 173, 190, 337, 787, 937
AsuNHI GCTAGC 1 cut(s) 67
AvaI CYCGRG 5 cut(s) 311, 374, 630, 861, 878
AvaII GGWCC 2 cut(s) 315, 1627
AxyI CCTNAGG 1 cut(s) 1376
BaeGI GKGCMC 2 cut(s) 336, 1005
BanII GRGCYC 1 cut(s) 521
Bbv12I GWGCWC 1 cut(s) 266
BbvI GCAGC 1 cut(s) 1305
BccI CCATC 9 cut(s) 209, 264, 314, 350, 431, 956, 1598, 1658, 1760
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 4 cut(s) 565, 599, 1713, 1747
BciT130I CCWGG 2 cut(s) 939, 997
BclI TGATCA 1 cut(s) 988
BcnI CCSGG 1 cut(s) 1607
BcoDI GTCTC 1 cut(s) 627
BcuI ACTAGT 1 cut(s) 1133
BfaI CTAG 4 cut(s) 68, 558, 1134, 1316
BfoI RGCGCY 1 cut(s) 273
BfuAI ACCTGC 3 cut(s) 631, 763, 1703
BglII AGATCT 1 cut(s) 142
BisI GCNGC 2 cut(s) 1294, 1499
BlsI GCNGC 2 cut(s) 1295, 1500
Bme1390I CCNGG 3 cut(s) 939, 997, 1607
Bme18I GGWCC 2 cut(s) 315, 1627
BmeT110I CYCGRG 5 cut(s) 311, 374, 630, 861, 878
BmgT120I GGNCC 2 cut(s) 315, 1627
BmiI GGNNCC 3 cut(s) 316, 365, 886
BmrFI CCNGG 3 cut(s) 939, 997, 1607
BmsI GCATC 5 cut(s) 255, 563, 728, 809, 1220
BmtI GCTAGC 1 cut(s) 71
BpmI CTGGAG 1 cut(s) 1253
BpuEI CTTGAG 1 cut(s) 1376
BpuMI CCSGG 1 cut(s) 1607
BsaAI YACGTR 1 cut(s) 979
BsaJI CCNNGG 5 cut(s) 527, 879, 937, 996, 1630
BsaXI ACNNNNNCTCC 3 cut(s) 1052, 1082, 1778
Bsc4I CCNNNNNNNGG 2 cut(s) 749, 1123
Bse1I ACTGG 2 cut(s) 956, 1236
Bse21I CCTNAGG 1 cut(s) 1376
Bse3DI GCAATG 2 cut(s) 636, 774
BseBI CCWGG 2 cut(s) 939, 997
BseDI CCNNGG 5 cut(s) 527, 879, 937, 996, 1630
BseGI GGATG 7 cut(s) 201, 246, 719, 933, 948, 1438, 1627
BseLI CCNNNNNNNGG 2 cut(s) 749, 1123
BseMI GCAATG 2 cut(s) 636, 774
BseMII CTCAG 1 cut(s) 1229
BseNI ACTGG 2 cut(s) 956, 1236
BseRI GAGGAG 1 cut(s) 156
BseSI GKGCMC 2 cut(s) 336, 1005
BseXI GCAGC 1 cut(s) 1305
BsgI GTGCAG 2 cut(s) 559, 1137
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 1 cut(s) 1501
BsiHKAI GWGCWC 1 cut(s) 266
BsiHKCI CYCGRG 5 cut(s) 311, 374, 630, 861, 878
BsiSI CCGG 1 cut(s) 1607
BslFI GGGAC 1 cut(s) 766
BslI CCNNNNNNNGG 2 cut(s) 749, 1123
BsmAI GTCTC 1 cut(s) 627
BsmFI GGGAC 1 cut(s) 766
BsmI GAATGC 3 cut(s) 576, 929, 1424
BsnI GGCC 1 cut(s) 1501
BsoBI CYCGRG 5 cut(s) 311, 374, 630, 861, 878
Bsp1286I GDGCHC 4 cut(s) 266, 336, 521, 1005
Bsp1407I TGTACA 1 cut(s) 1165
Bsp143I GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
BspACI CCGC 3 cut(s) 348, 1498, 1510
BspANI GGCC 1 cut(s) 1501
BspCNI CTCAG 1 cut(s) 1228
BspFNI CGCG 1 cut(s) 428
BspHI TCATGA 1 cut(s) 1203
BspLI GGNNCC 3 cut(s) 316, 365, 886
BspMI ACCTGC 3 cut(s) 631, 763, 1703
BspOI GCTAGC 1 cut(s) 71
BspPI GGATC 3 cut(s) 187, 560, 1486
BspQI GCTCTTC 2 cut(s) 498, 509
BsrDI GCAATG 2 cut(s) 636, 774
BsrGI TGTACA 1 cut(s) 1165
BsrI ACTGG 2 cut(s) 956, 1236
BssECI CCNNGG 5 cut(s) 527, 879, 937, 996, 1630
BssMI GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
Bst2UI CCWGG 2 cut(s) 939, 997
Bst4CI ACNGT 4 cut(s) 174, 331, 1044, 1638
Bst6I CTCTTC 4 cut(s) 498, 509, 587, 1407
BstAUI TGTACA 1 cut(s) 1165
BstBAI YACGTR 1 cut(s) 979
BstC8I GCNNGC 3 cut(s) 69, 419, 816
BstDEI CTNAG 4 cut(s) 1215, 1376, 1385, 1700
BstDSI CCRYGG 1 cut(s) 1630
BstF5I GGATG 7 cut(s) 201, 246, 719, 933, 948, 1438, 1627
BstFNI CGCG 1 cut(s) 428
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 3 cut(s) 272, 430, 1486
BstKTI GATC 8 cut(s) 145, 181, 195, 408, 555, 759, 991, 1481
BstMAI GTCTC 1 cut(s) 627
BstMBI GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
BstMWI GCNNNNNNNGC 4 cut(s) 84, 427, 436, 1741
BstNI CCWGG 2 cut(s) 939, 997
BstNSI RCATGY 3 cut(s) 818, 1490, 1601
BstSCI CCNGG 3 cut(s) 937, 995, 1605
BstSLI GKGCMC 2 cut(s) 336, 1005
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 1 cut(s) 1305
BstX2I RGATCY 1 cut(s) 142
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 1 cut(s) 142
Bsu36I CCTNAGG 1 cut(s) 1376
BsuRI GGCC 1 cut(s) 1501
BtgI CCRYGG 1 cut(s) 1630
BtgZI GCGATG 1 cut(s) 409
BtsCI GGATG 7 cut(s) 201, 246, 719, 933, 948, 1438, 1627
BtsIMutI CAGTG 2 cut(s) 829, 1251
BveI ACCTGC 3 cut(s) 631, 763, 1703
Cac8I GCNNGC 3 cut(s) 69, 419, 816
CaiI CAGNNNCTG 1 cut(s) 1518
CciI TCATGA 1 cut(s) 1203
CfoI GCGC 3 cut(s) 272, 430, 1486
Cfr13I GGNCC 2 cut(s) 315, 1627
Csp6I GTAC 6 cut(s) 19, 567, 965, 980, 1069, 1166
CviQI GTAC 6 cut(s) 19, 567, 965, 980, 1069, 1166
DdeI CTNAG 4 cut(s) 1215, 1376, 1385, 1700
DpnI GATC 8 cut(s) 144, 180, 194, 407, 554, 758, 990, 1480
DpnII GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
EaeI YGGCCR 1 cut(s) 1499
Eam1104I CTCTTC 4 cut(s) 498, 509, 587, 1407
EarI CTCTTC 4 cut(s) 498, 509, 587, 1407
Eco24I GRGCYC 1 cut(s) 521
Eco32I GATATC 2 cut(s) 905, 971
Eco47I GGWCC 2 cut(s) 315, 1627
Eco57I CTGAAG 2 cut(s) 479, 1227
Eco81I CCTNAGG 1 cut(s) 1376
Eco88I CYCGRG 5 cut(s) 311, 374, 630, 861, 878
EcoRII CCWGG 2 cut(s) 937, 995
EcoRV GATATC 2 cut(s) 905, 971
EcoT22I ATGCAT 1 cut(s) 578
EcoT38I GRGCYC 1 cut(s) 521
FalI AAGNNNNNCTT 2 cut(s) 1454, 1486
FaqI GGGAC 1 cut(s) 766
FauNDI CATATG 1 cut(s) 1183
FbaI TGATCA 1 cut(s) 988
Fnu4HI GCNGC 2 cut(s) 1294, 1499
FokI GGATG 7 cut(s) 188, 233, 706, 920, 935, 1445, 1634
FriOI GRGCYC 1 cut(s) 521
Fsp4HI GCNGC 2 cut(s) 1294, 1499
FspBI CTAG 4 cut(s) 68, 558, 1134, 1316
GlaI GCGC 3 cut(s) 271, 429, 1485
GluI GCNGC 2 cut(s) 1294, 1499
GsuI CTGGAG 1 cut(s) 1253
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 1 cut(s) 1501
HapII CCGG 1 cut(s) 1607
HhaI GCGC 3 cut(s) 272, 430, 1486
Hin6I GCGC 3 cut(s) 270, 428, 1484
HinP1I GCGC 3 cut(s) 270, 428, 1484
HincII GTYRAC 2 cut(s) 327, 787
HindII GTYRAC 2 cut(s) 327, 787
HindIII AAGCTT 1 cut(s) 840
HinfI GANTC 8 cut(s) 224, 725, 734, 1336, 1389, 1586, 1652, 1671
HpaI GTTAAC 1 cut(s) 787
HpaII CCGG 1 cut(s) 1607
HphI GGTGA 6 cut(s) 139, 173, 190, 337, 787, 937
Hpy166II GTNNAC 7 cut(s) 327, 787, 982, 1038, 1309, 1360, 1634
Hpy188III TCNNGA 6 cut(s) 376, 832, 878, 1204, 1316, 1340
Hpy8I GTNNAC 7 cut(s) 327, 787, 982, 1038, 1309, 1360, 1634
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 4 cut(s) 15, 166, 499, 1251
HpyCH4III ACNGT 4 cut(s) 174, 331, 1044, 1638
HpyCH4IV ACGT 4 cut(s) 978, 1161, 1457, 1563
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 427, 436, 1741
HpyF3I CTNAG 4 cut(s) 1215, 1376, 1385, 1700
HpySE526I ACGT 4 cut(s) 978, 1161, 1457, 1563
HspAI GCGC 3 cut(s) 270, 428, 1484
Ksp22I TGATCA 1 cut(s) 988
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
LguI GCTCTTC 2 cut(s) 498, 509
LmnI GCTCC 4 cut(s) 108, 631, 646, 1234
Lsp1109I GCAGC 1 cut(s) 1305
LweI GCATC 5 cut(s) 255, 563, 728, 809, 1220
MaeI CTAG 4 cut(s) 68, 558, 1134, 1316
MaeII ACGT 4 cut(s) 978, 1161, 1457, 1563
MalI GATC 8 cut(s) 144, 180, 194, 407, 554, 758, 990, 1480
MboI GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
MboII GAAGA 8 cut(s) 485, 526, 574, 676, 1424, 1680, 1694, 1775
MfeI CAATTG 1 cut(s) 711
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 4 cut(s) 266, 336, 521, 1005
MluCI AATT 8 cut(s) 411, 485, 698, 711, 1051, 1223, 1523, 1577
MlyI GAGTC 2 cut(s) 1383, 1595
MmeI TCCRAC 1 cut(s) 976
Mph1103I ATGCAT 1 cut(s) 578
MroXI GAANNNNTTC 1 cut(s) 841
MseI TTAA 7 cut(s) 128, 401, 786, 1193, 1461, 1494, 1746
MslI CAYNNNNRTG 3 cut(s) 128, 1182, 1365
MspI CCGG 1 cut(s) 1607
MspR9I CCNGG 3 cut(s) 939, 997, 1607
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 3 cut(s) 576, 929, 1424
MvaI CCWGG 2 cut(s) 939, 997
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 4 cut(s) 84, 427, 436, 1741
NciI CCSGG 1 cut(s) 1607
NdeI CATATG 1 cut(s) 1183
NdeII GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
NheI GCTAGC 1 cut(s) 67
NlaIV GGNNCC 3 cut(s) 316, 365, 886
NmeAIII GCCGAG 1 cut(s) 552
NsiI ATGCAT 1 cut(s) 578
NspI RCATGY 3 cut(s) 818, 1490, 1601
PaeI GCATGC 1 cut(s) 818
PaeR7I CTCGAG 2 cut(s) 374, 861
PagI TCATGA 1 cut(s) 1203
PaqCI CACCTGC 1 cut(s) 763
PciSI GCTCTTC 2 cut(s) 498, 509
PctI GAATGC 3 cut(s) 576, 929, 1424
PdmI GAANNNNTTC 1 cut(s) 841
PfeI GAWTC 6 cut(s) 224, 725, 734, 1336, 1652, 1671
PfoI TCCNGGA 1 cut(s) 1605
PkrI GCNGC 2 cut(s) 1295, 1500
PleI GAGTC 2 cut(s) 1383, 1594
PpsI GAGTC 2 cut(s) 1383, 1594
Ppu21I YACGTR 1 cut(s) 979
Psp1406I AACGTT 2 cut(s) 1161, 1457
Psp6I CCWGG 2 cut(s) 937, 995
PspGI CCWGG 2 cut(s) 937, 995
PspN4I GGNNCC 3 cut(s) 316, 365, 886
PspPI GGNCC 2 cut(s) 315, 1627
PspXI VCTCGAGB 1 cut(s) 861
PstNI CAGNNNCTG 1 cut(s) 1518
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 6 cut(s) 20, 568, 966, 981, 1070, 1167
RsaNI GTAC 6 cut(s) 19, 567, 965, 980, 1069, 1166
RseI CAYNNNNRTG 3 cut(s) 128, 1182, 1365
SapI GCTCTTC 2 cut(s) 498, 509
SaqAI TTAA 7 cut(s) 128, 401, 786, 1193, 1461, 1494, 1746
SatI GCNGC 2 cut(s) 1294, 1499
Sau3AI GATC 8 cut(s) 142, 178, 192, 405, 552, 756, 988, 1478
Sau96I GGNCC 2 cut(s) 315, 1627
SchI GAGTC 2 cut(s) 1383, 1595
ScrFI CCNGG 3 cut(s) 939, 997, 1607
SduI GDGCHC 4 cut(s) 266, 336, 521, 1005
SfaNI GCATC 5 cut(s) 255, 563, 728, 809, 1220
Sfr274I CTCGAG 2 cut(s) 374, 861
SinI GGWCC 2 cut(s) 315, 1627
SlaI CTCGAG 2 cut(s) 374, 861
SmiMI CAYNNNNRTG 3 cut(s) 128, 1182, 1365
SmlI CTYRAG 3 cut(s) 374, 861, 1391
SmoI CTYRAG 3 cut(s) 374, 861, 1391
SpeI ACTAGT 1 cut(s) 1133
SphI GCATGC 1 cut(s) 818
Sse9I AATT 8 cut(s) 411, 485, 698, 711, 1051, 1223, 1523, 1577
SsiI CCGC 3 cut(s) 348, 1498, 1510
SspI AATATT 1 cut(s) 37
SspMI CTAG 4 cut(s) 68, 558, 1134, 1316
StyD4I CCNGG 3 cut(s) 937, 995, 1605
TaaI ACNGT 4 cut(s) 174, 331, 1044, 1638
TaiI ACGT 4 cut(s) 981, 1164, 1460, 1566
TaqI TCGA 3 cut(s) 375, 571, 862
TasI AATT 8 cut(s) 411, 485, 698, 711, 1051, 1223, 1523, 1577
TatI WGTACW 2 cut(s) 566, 1165
TauI GCSGC 1 cut(s) 1501
TfiI GAWTC 6 cut(s) 224, 725, 734, 1336, 1652, 1671
Tru1I TTAA 7 cut(s) 128, 401, 786, 1193, 1461, 1494, 1746
Tru9I TTAA 7 cut(s) 128, 401, 786, 1193, 1461, 1494, 1746
TscAI CASTG 2 cut(s) 829, 1251
TseI GCWGC 1 cut(s) 1293
TspGWI ACGGA 1 cut(s) 1619
TspRI CASTG 2 cut(s) 829, 1251
VpaK11BI GGWCC 2 cut(s) 315, 1627
XapI RAATTY 2 cut(s) 411, 1577
XbaI TCTAGA 1 cut(s) 1315
XceI RCATGY 3 cut(s) 818, 1490, 1601
XhoI CTCGAG 2 cut(s) 374, 861
XmnI GAANNNNTTC 1 cut(s) 841
XspI CTAG 4 cut(s) 68, 558, 1134, 1316
Zsp2I ATGCAT 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.