MD02G1204100.v1.1

Protein of unknown function (DUF1499)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010909
Physical Location & Seq
Reverse (-)
20384393 .. 20387462
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1204100.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGCGTACCCAGCAAGCTCCTCCTCCTCCTACAACCACTACAAGAATTACAGCAAACTGAAACAGCGTACCAGTTTCTCCATTCCCCATCGTATCATAGTTTCTCATAAGCAGCAGCAGCAGCAGCAAAACGATCATAAACCCACCGACCCAATTGGTCGAAGGGAAATAATACTGAGGAGCAGCGAATTGGCAGTGGTAGGAGCCATCTTCAACTTGAGTGGTAAAAAGCCCGAGTATCTTGGGGTGCAGAAAAATCAGCAAGCTTTGGCTCTCTGCCCAGCTACTAAGAATTGCATATCAACCTCTGAAAATGTCAGTGACCTTACCCATTATGCCCCTCCCTGGAACTACAATGGAAGTAGGAAAAAGCCGGTCAGCAGAGAAGTTGCGATAGAAGAACTTCTCCAAGTGATTAAATCGACAAAGCCAGACAAGTTCACACCACAGATCATGGAGAAGAGAGACGACTATGTTCGTGTGGAGTACCAAAGCGCTATTTTAGGGCTTGTGGATGATGTAGAGTTTTGGTTTCCACCAGGCACCGACTCAATAGTCGAGTATCGATCGGCATCCCGGTTGGGGATATCCGATTTCAACATCAACAGAAAAAGAATCAAGGCATTGCGGCAGGAGTTGGAGAAGAAAGGTTGGGCGTCGAAAGACAGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1499

Protein Analysis

225

Amino Acids

25.62

Weight (kDa)

9.52

Isoelectric Point (pI)

58.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1499 PF07386 90 - 213 3e-33 Protein of unknown function (DUF1499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010909)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60810 AT3G60810
fragaria_vesca FvH4_7g11910 FvH4_7g11910 FvH4_7g11910
malus_domestica MD02G1204100.v1.1
prunus_persica Prupe.2G145700_v2.0.a1 Prupe.2G145700_v2.0.a1
pyrus_communis pycom02g16640
rosa_chinensis RchiOBHm_Chr1g0350591
rosa_laevigata RLG00000028470
rosa_multiflora Rmu_sc0009041.1_g000002 Rmu_sc0014368.1_g000005
rosa_roxburghii Rroxscaffold_4G00304690
rosa_rugosa Rorug01G0212400
rosa_samantha Rh1AG226900 Rh1BG195600 Rh1CG211700 Rh1DG223700
rosa_wichuraiana Rw1G019610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AccB1I GGYRCC 1 cut(s) 542
AciI CCGC 1 cut(s) 628
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 3 cut(s) 8, 70, 488
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 2 cut(s) 345, 582
AgsI TTSAA 2 cut(s) 214, 598
AjnI CCWGG 2 cut(s) 344, 538
AluBI AGCT 4 cut(s) 18, 266, 284, 669
AluI AGCT 4 cut(s) 18, 266, 284, 669
Alw26I GTCTC 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 233
Aor51HI AGCGCT 1 cut(s) 496
ApeKI GCWGC 6 cut(s) 112, 115, 118, 121, 124, 183
Asp700I GAANNNNTTC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 497
AsuC2I CCSGG 1 cut(s) 577
AvaI CYCGRG 1 cut(s) 233
BanI GGYRCC 1 cut(s) 542
BbvI GCAGC 6 cut(s) 124, 127, 130, 133, 136, 195
BccI CCATC 2 cut(s) 96, 215
BciT130I CCWGG 2 cut(s) 346, 540
BcnI CCSGG 1 cut(s) 577
BcoDI GTCTC 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 498
BisI GCNGC 7 cut(s) 113, 116, 119, 122, 125, 184, 629
BlsI GCNGC 7 cut(s) 114, 117, 120, 123, 126, 185, 630
Bme1390I CCNGG 3 cut(s) 346, 540, 577
BmeT110I CYCGRG 1 cut(s) 233
BmiI GGNNCC 2 cut(s) 205, 544
BmrFI CCNGG 3 cut(s) 346, 540, 577
BmsI GCATC 1 cut(s) 581
BpuEI CTTGAG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 577
Bsa29I ATCGAT 1 cut(s) 565
BsaBI GATNNNNATC 1 cut(s) 571
BsaHI GRCGYC 1 cut(s) 656
BsaJI CCNNGG 1 cut(s) 344
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 582
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 623
Bse8I GATNNNNATC 1 cut(s) 571
BseBI CCWGG 2 cut(s) 346, 540
BseCI ATCGAT 1 cut(s) 565
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 520, 572
BseJI GATNNNNATC 1 cut(s) 571
BseLI CCNNNNNNNGG 2 cut(s) 345, 582
BseMI GCAATG 1 cut(s) 623
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 4 cut(s) 10, 13, 16, 193
BseXI GCAGC 6 cut(s) 124, 127, 130, 133, 136, 195
BseYI CCCAGC 2 cut(s) 10, 280
BsgI GTGCAG 1 cut(s) 269
Bsh1285I CGRYCG 1 cut(s) 569
BshNI GGYRCC 1 cut(s) 542
BshVI ATCGAT 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 2 cut(s) 374, 577
BslI CCNNNNNNNGG 2 cut(s) 345, 582
BsmAI GTCTC 1 cut(s) 459
BsmBI CGTCTC 1 cut(s) 459
BsoBI CYCGRG 1 cut(s) 233
Bsp143I GATC 3 cut(s) 133, 450, 566
BspACI CCGC 1 cut(s) 628
BspCNI CTCAG 1 cut(s) 168
BspDI ATCGAT 1 cut(s) 565
BspLI GGNNCC 2 cut(s) 205, 544
BspT107I GGYRCC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 623
BsrFI RCCGGY 1 cut(s) 373
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 3 cut(s) 133, 450, 566
BssNI GRCGYC 1 cut(s) 656
Bst2UI CCWGG 2 cut(s) 346, 540
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 656
BstC8I GCNNGC 2 cut(s) 16, 264
BstDEI CTNAG 2 cut(s) 176, 288
BstF5I GGATG 2 cut(s) 520, 572
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 3 cut(s) 136, 453, 569
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 3 cut(s) 133, 450, 566
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 4 cut(s) 11, 118, 121, 124
BstNI CCWGG 2 cut(s) 346, 540
BstSCI CCNGG 3 cut(s) 344, 538, 575
BstV1I GCAGC 6 cut(s) 124, 127, 130, 133, 136, 195
Bsu15I ATCGAT 1 cut(s) 565
BsuTUI ATCGAT 1 cut(s) 565
BtsCI GGATG 2 cut(s) 520, 572
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 201, 325
Cac8I GCNNGC 2 cut(s) 16, 264
CfoI GCGC 1 cut(s) 497
Cfr10I RCCGGY 1 cut(s) 373
ClaI ATCGAT 1 cut(s) 565
CseI GACGC 1 cut(s) 645
Csp6I GTAC 3 cut(s) 7, 69, 487
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviAII CATG 1 cut(s) 454
CviQI GTAC 3 cut(s) 7, 69, 487
DdeI CTNAG 2 cut(s) 176, 288
DpnI GATC 3 cut(s) 135, 452, 568
DpnII GATC 3 cut(s) 133, 450, 566
DrdI GACNNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 554
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco32I GATATC 1 cut(s) 588
Eco47III AGCGCT 1 cut(s) 496
Eco88I CYCGRG 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 344, 538
EcoRV GATATC 1 cut(s) 588
Esp3I CGTCTC 1 cut(s) 459
FaeI CATG 1 cut(s) 457
FaiI YATR 7 cut(s) 98, 108, 138, 299, 336, 455, 474
FatI CATG 1 cut(s) 453
Fnu4HI GCNGC 7 cut(s) 113, 116, 119, 122, 125, 184, 629
FokI GGATG 2 cut(s) 527, 559
Fsp4HI GCNGC 7 cut(s) 113, 116, 119, 122, 125, 184, 629
GlaI GCGC 1 cut(s) 496
GluI GCNGC 7 cut(s) 113, 116, 119, 122, 125, 184, 629
GsaI CCCAGC 2 cut(s) 14, 284
HaeII RGCGCY 1 cut(s) 498
HapII CCGG 2 cut(s) 374, 577
HgaI GACGC 1 cut(s) 645
HhaI GCGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 1 cut(s) 457
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 2 cut(s) 548, 615
HpaII CCGG 2 cut(s) 374, 577
Hpy166II GTNNAC 1 cut(s) 441
Hpy188I TCNGA 2 cut(s) 310, 592
Hpy8I GTNNAC 1 cut(s) 441
Hpy99I CGWCG 1 cut(s) 661
HpyAV CCTTC 1 cut(s) 156
HpyCH4V TGCA 2 cut(s) 250, 297
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 118, 121, 124
HpyF3I CTNAG 2 cut(s) 176, 288
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 1 cut(s) 457
HspAI GCGC 1 cut(s) 495
Kzo9I GATC 3 cut(s) 133, 450, 566
LmnI GCTCC 3 cut(s) 23, 180, 203
Lsp1109I GCAGC 6 cut(s) 124, 127, 130, 133, 136, 195
LweI GCATC 1 cut(s) 581
MaeIII GTNAC 1 cut(s) 320
MalI GATC 3 cut(s) 135, 452, 568
MboI GATC 3 cut(s) 133, 450, 566
MboII GAAGA 4 cut(s) 202, 410, 472, 655
MfeI CAATTG 1 cut(s) 153
MluCI AATT 4 cut(s) 46, 153, 188, 292
MlyI GAGTC 1 cut(s) 542
MmeI TCCRAC 1 cut(s) 618
MnlI CCTC 6 cut(s) 31, 34, 37, 171, 316, 351
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 1 cut(s) 417
MspI CCGG 2 cut(s) 374, 577
MspR9I CCNGG 3 cut(s) 346, 540, 577
MunI CAATTG 1 cut(s) 153
MvaI CCWGG 2 cut(s) 346, 540
MwoI GCNNNNNNNGC 4 cut(s) 11, 118, 121, 124
NciI CCSGG 1 cut(s) 577
NdeII GATC 3 cut(s) 133, 450, 566
NlaIII CATG 1 cut(s) 457
NlaIV GGNNCC 2 cut(s) 205, 544
NmuCI GTSAC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 1 cut(s) 615
PkrI GCNGC 7 cut(s) 114, 117, 120, 123, 126, 185, 630
Ple19I CGATCG 1 cut(s) 569
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
Psp6I CCWGG 2 cut(s) 344, 538
PspFI CCCAGC 2 cut(s) 10, 280
PspGI CCWGG 2 cut(s) 344, 538
PspN4I GGNNCC 2 cut(s) 205, 544
PvuI CGATCG 1 cut(s) 569
RsaI GTAC 3 cut(s) 8, 70, 488
RsaNI GTAC 3 cut(s) 7, 69, 487
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 7 cut(s) 113, 116, 119, 122, 125, 184, 629
Sau3AI GATC 3 cut(s) 133, 450, 566
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 3 cut(s) 346, 540, 577
SetI ASST 7 cut(s) 20, 268, 286, 308, 327, 652, 671
SfaNI GCATC 1 cut(s) 581
SmlI CTYRAG 1 cut(s) 217
SmoI CTYRAG 1 cut(s) 217
Sse9I AATT 4 cut(s) 46, 153, 188, 292
SsiI CCGC 1 cut(s) 628
StyD4I CCNGG 3 cut(s) 344, 538, 575
TaqI TCGA 5 cut(s) 160, 422, 558, 565, 659
TasI AATT 4 cut(s) 46, 153, 188, 292
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 2 cut(s) 201, 325
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 6 cut(s) 112, 115, 118, 121, 124, 183
Tsp45I GTSAC 1 cut(s) 320
TspRI CASTG 2 cut(s) 201, 325
XmnI GAANNNNTTC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.