Rorug01G0212400

Protein of unknown function (DUF1499)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010909
Physical Location & Seq
Reverse (-)
30244908 .. 30248269
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0212400.1

Sequence Viewer

Length: 1248 bp
ATGCACCAGTTCATGTCCCCAGTATCTATCTCTCTTTGTTCGTCTCAAAAATCGTTACATTCAAACATTCACTCCCATTCAACTCCTGCATGCACTTCTCCTCCAGATTCCATGATGATGAGCAATTATGGAGGCCAACGACTACTAGTGGTCGACTGTTGCTATTGCCAGACCCAACTCCAGCTGCCTTCTGTGAGTCCGATATACATCGTCGGCACATCCGTCCGCTGCGGCCGCTGCTATGCCAGTACCCGCGTCGCCTCTCCGGGCTTCCCCCGCTCCCCTTATAGTGCTGTACCCTACTATGTCCCTATAAACCCACCACCACACGCCGGCTTCCCCCACAGATCACCAGGACCTCCGCCAAACGTGCACAGGCAGAAGAAGGCGGTGATCTGCGGGATATCGTATAGGTACTCGAGGCATGAGCTCAAAGGTAGCATCAATGACGCCAAAAACATGCGGTATCTCCTCATGAGCAAGTTCAAGTTTCCAGAAGATTCCATTTGCATGCTCACTGAAGAAGAAACTCACCCCTATAAGATTCCATATAAAAACAACATTAGAATGGCATTAGATTGGCTTGTACAAGGATGTCAACCTGGTGACTCCCTTGTGTTTCACTACTCTGGTCATGGTTCACGGCAGTTGGATTATGATGGTGATGAAGTTGATGGATATGATGAAACCCTTTGTCCCCTTGACTTCGAAACCCAGGGTATGATTGATGATGATGAGATAAATGCAGCAATTGTTAGGCCCATTCCACATGGGGTCAAGCTTCATGCAATTATAGATTCTTGTCATAGTGGCACCATACTGGATTTGCCATTCCTTTGCAGAATGAACATGGGTGGAATATATGTATGGGAGGATCATCGCCCTCCATCAGGCGTATGGAAAGGAACAAGTGGTGGAGAAGTTATATCTATCAGTGGTTGTGATGACTATCAAACGTCTGCGGAAACATCAGCTCTATCCAAGATCACATCAACAGGTGCCATGACTTTCTGCTTCATCCAAGCAATCGAGCATGGACAGGCAGCCACATATGGGAGCTTACTCAATTCTATGCGTAGTACCATTCGAAGTATGGGTTCTGCCGGTGGTGGTAGTGGTGGTGCTGTTACATCTCTTGCCGGCATGCTTCTAATAGGCGGAAATGTTGGTGGTGGTAGGCTAAGACAGGAGCCTCAATTAACTGCCTGCGAGCCATTCGATGTGTACACGAAACCTTTGGCCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1499

Protein Analysis

415

Amino Acids

45.16

Weight (kDa)

6.45

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 128 - 402 6.4e-32 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010909)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60810 AT3G60810
fragaria_vesca FvH4_7g11910 FvH4_7g11910 FvH4_7g11910
malus_domestica MD02G1204100.v1.1
prunus_persica Prupe.2G145700_v2.0.a1 Prupe.2G145700_v2.0.a1
pyrus_communis pycom02g16640
rosa_chinensis RchiOBHm_Chr1g0350591
rosa_laevigata RLG00000028470
rosa_multiflora Rmu_sc0009041.1_g000002 Rmu_sc0014368.1_g000005
rosa_roxburghii Rroxscaffold_4G00304690
rosa_rugosa Rorug01G0212400
rosa_samantha Rh1AG226900 Rh1BG195600 Rh1CG211700 Rh1DG223700
rosa_wichuraiana Rw1G019610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 812, 998
AccBSI CCGCTC 1 cut(s) 279
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 255
AclWI GGATC 1 cut(s) 882
AcoI YGGCCR 1 cut(s) 232
AcuI CTGAAG 1 cut(s) 540
AcyI GRCGYC 1 cut(s) 450
AfaI GTAC 6 cut(s) 250, 297, 416, 588, 1081, 1226
AfiI CCNNNNNNNGG 3 cut(s) 332, 890, 1053
AgsI TTSAA 3 cut(s) 63, 81, 487
AhlI ACTAGT 1 cut(s) 145
AjnI CCWGG 3 cut(s) 352, 601, 714
AluBI AGCT 5 cut(s) 184, 430, 781, 974, 1059
AluI AGCT 5 cut(s) 184, 430, 781, 974, 1059
Alw21I GWGCWC 2 cut(s) 375, 432
Alw26I GTCTC 1 cut(s) 48
Alw44I GTGCAC 1 cut(s) 371
AlwI GGATC 1 cut(s) 882
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 4 cut(s) 133, 232, 758, 1239
ApaLI GTGCAC 1 cut(s) 371
ApeKI GCWGC 5 cut(s) 184, 228, 237, 746, 1043
AspS9I GGNCC 3 cut(s) 356, 759, 1240
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 5 cut(s) 342, 403, 524, 617, 674
AsuII TTCGAA 2 cut(s) 708, 1087
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 375
BanI GGYRCC 2 cut(s) 812, 998
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 375, 432
BbvI GCAGC 5 cut(s) 171, 215, 224, 758, 1055
BccI CCATC 3 cut(s) 653, 668, 895
BceAI ACGGC 1 cut(s) 659
BciT130I CCWGG 3 cut(s) 354, 603, 716
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 48
BcuI ACTAGT 1 cut(s) 145
BfaI CTAG 1 cut(s) 146
BisI GCNGC 7 cut(s) 185, 229, 232, 235, 238, 747, 1044
BlsI GCNGC 7 cut(s) 186, 230, 233, 236, 239, 748, 1045
Bme1390I CCNGG 4 cut(s) 267, 354, 603, 716
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 3 cut(s) 356, 759, 1240
BmiI GGNNCC 3 cut(s) 814, 1000, 1191
BmrFI CCNGG 4 cut(s) 267, 354, 603, 716
BmrI ACTGGG 1 cut(s) 14
BmsI GCATC 1 cut(s) 450
BmuI ACTGGG 1 cut(s) 14
BpmI CTGGAG 2 cut(s) 87, 164
Bpu14I TTCGAA 2 cut(s) 708, 1087
BpuMI CCSGG 1 cut(s) 267
BsaBI GATNNNNATC 2 cut(s) 206, 948
BsaHI GRCGYC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 714, 715
BsaXI ACNNNNNCTCC 4 cut(s) 56, 85, 86, 115
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 890, 1053
Bse118I RCCGGY 3 cut(s) 332, 1103, 1139
Bse1I ACTGG 4 cut(s) 7, 20, 246, 825
Bse8I GATNNNNATC 2 cut(s) 206, 948
BseBI CCWGG 3 cut(s) 354, 603, 716
BseDI CCNNGG 2 cut(s) 714, 715
BseGI GGATG 3 cut(s) 218, 599, 1017
BseJI GATNNNNATC 2 cut(s) 206, 948
BseLI CCNNNNNNNGG 3 cut(s) 332, 890, 1053
BseNI ACTGG 4 cut(s) 7, 20, 246, 825
BseRI GAGGAG 2 cut(s) 90, 461
BseSI GKGCMC 1 cut(s) 375
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 5 cut(s) 171, 215, 224, 758, 1055
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 4 cut(s) 135, 234, 760, 1241
BshNI GGYRCC 2 cut(s) 812, 998
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 375, 432
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 4 cut(s) 266, 333, 1104, 1140
BslFI GGGAC 2 cut(s) 293, 681
BslI CCNNNNNNNGG 3 cut(s) 332, 890, 1053
BsmAI GTCTC 1 cut(s) 48
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 2 cut(s) 293, 681
BsnI GGCC 4 cut(s) 135, 234, 760, 1241
BsoBI CYCGRG 1 cut(s) 418
Bsp119I TTCGAA 2 cut(s) 708, 1087
Bsp1286I GDGCHC 2 cut(s) 375, 432
Bsp1407I TGTACA 2 cut(s) 586, 1224
Bsp143I GATC 4 cut(s) 347, 393, 874, 984
BspANI GGCC 4 cut(s) 135, 234, 760, 1241
BspFNI CGCG 1 cut(s) 255
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 814, 1000, 1191
BspPI GGATC 1 cut(s) 882
BspT104I TTCGAA 2 cut(s) 708, 1087
BspT107I GGYRCC 2 cut(s) 812, 998
BsrBI CCGCTC 1 cut(s) 279
BsrFI RCCGGY 3 cut(s) 332, 1103, 1139
BsrGI TGTACA 2 cut(s) 586, 1224
BsrI ACTGG 4 cut(s) 7, 20, 246, 825
BssAI RCCGGY 3 cut(s) 332, 1103, 1139
BssECI CCNNGG 2 cut(s) 714, 715
BssMI GATC 4 cut(s) 347, 393, 874, 984
BssNI GRCGYC 1 cut(s) 450
Bst2UI CCWGG 3 cut(s) 354, 603, 716
Bst4CI ACNGT 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 450
BstAUI TGTACA 2 cut(s) 586, 1224
BstBI TTCGAA 2 cut(s) 708, 1087
BstC8I GCNNGC 7 cut(s) 91, 334, 512, 1141, 1145, 1207, 1211
BstDEI CTNAG 1 cut(s) 1181
BstENI CCTNNNNNAGG 1 cut(s) 888
BstF5I GGATG 3 cut(s) 218, 599, 1017
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 4 cut(s) 350, 396, 877, 987
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 4 cut(s) 347, 393, 874, 984
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 4 cut(s) 234, 237, 276, 370
BstNI CCWGG 3 cut(s) 354, 603, 716
BstNSI RCATGY 4 cut(s) 93, 463, 514, 1147
BstSCI CCNGG 4 cut(s) 265, 352, 601, 714
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 5 cut(s) 171, 215, 224, 758, 1055
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 4 cut(s) 135, 234, 760, 1241
BtgZI GCGATG 1 cut(s) 863
BtsCI GGATG 3 cut(s) 218, 599, 1017
BtsIMutI CAGTG 2 cut(s) 516, 940
Cac8I GCNNGC 7 cut(s) 91, 334, 512, 1141, 1145, 1207, 1211
CciI TCATGA 1 cut(s) 474
CciNI GCGGCCGC 1 cut(s) 232
Cfr10I RCCGGY 3 cut(s) 332, 1103, 1139
Cfr13I GGNCC 3 cut(s) 356, 759, 1240
CseI GACGC 2 cut(s) 244, 458
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 6 cut(s) 249, 296, 415, 587, 1080, 1225
CviQI GTAC 6 cut(s) 249, 296, 415, 587, 1080, 1225
DdeI CTNAG 1 cut(s) 1181
DpnI GATC 4 cut(s) 349, 395, 876, 986
DpnII GATC 4 cut(s) 347, 393, 874, 984
EaeI YGGCCR 1 cut(s) 232
EagI CGGCCG 1 cut(s) 232
EciI GGCGGA 2 cut(s) 351, 1173
Ecl136II GAGCTC 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 356
Eco52I CGGCCG 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 418
EcoICRI GAGCTC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 888
EcoO109I RGGNCCY 1 cut(s) 356
EcoRII CCWGG 3 cut(s) 352, 601, 714
EcoRV GATATC 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 432
Esp3I CGTCTC 1 cut(s) 48
FaqI GGGAC 2 cut(s) 293, 681
FauI CCCGC 3 cut(s) 260, 284, 392
FauNDI CATATG 1 cut(s) 1051
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 7 cut(s) 185, 229, 232, 235, 238, 747, 1044
FokI GGATG 3 cut(s) 205, 606, 1004
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 7 cut(s) 185, 229, 232, 235, 238, 747, 1044
FspBI CTAG 1 cut(s) 146
GluI GCNGC 7 cut(s) 185, 229, 232, 235, 238, 747, 1044
GsuI CTGGAG 2 cut(s) 87, 164
HaeIII GGCC 4 cut(s) 135, 234, 760, 1241
HapII CCGG 4 cut(s) 266, 333, 1104, 1140
HgaI GACGC 2 cut(s) 244, 458
Hin1I GRCGYC 1 cut(s) 450
HincII GTYRAC 2 cut(s) 154, 599
HindII GTYRAC 2 cut(s) 154, 599
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 6 cut(s) 107, 196, 500, 544, 608, 797
HpaII CCGG 4 cut(s) 266, 333, 1104, 1140
HphI GGTGA 5 cut(s) 342, 403, 524, 617, 674
Hpy166II GTNNAC 6 cut(s) 154, 373, 599, 641, 1225, 1227
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 3 cut(s) 104, 475, 494
Hpy8I GTNNAC 6 cut(s) 154, 373, 599, 641, 1225, 1227
Hpy99I CGWCG 2 cut(s) 215, 260
HpyAV CCTTC 2 cut(s) 198, 379
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 2 cut(s) 369, 956
HpyCH4V TGCA 8 cut(s) 4, 89, 93, 373, 510, 746, 788, 840
HpyF10VI GCNNNNNNNGC 4 cut(s) 234, 237, 276, 370
HpyF3I CTNAG 1 cut(s) 1181
HpySE526I ACGT 2 cut(s) 369, 956
Hsp92I GRCGYC 1 cut(s) 450
KroI GCCGGC 2 cut(s) 332, 1139
KroNI GCCGGC 2 cut(s) 334, 1141
Kzo9I GATC 4 cut(s) 347, 393, 874, 984
LmnI GCTCC 3 cut(s) 284, 1056, 1189
Lsp1109I GCAGC 5 cut(s) 171, 215, 224, 758, 1055
LweI GCATC 1 cut(s) 450
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 1 cut(s) 146
MaeII ACGT 2 cut(s) 369, 956
MaeIII GTNAC 3 cut(s) 54, 605, 1126
MalI GATC 4 cut(s) 349, 395, 876, 986
MbiI CCGCTC 1 cut(s) 279
MboI GATC 4 cut(s) 347, 393, 874, 984
MboII GAAGA 4 cut(s) 394, 509, 533, 536
MfeI CAATTG 1 cut(s) 750
MhlI GDGCHC 2 cut(s) 375, 432
MluCI AATT 5 cut(s) 124, 750, 789, 1066, 1196
MlyI GAGTC 2 cut(s) 205, 602
MmeI TCCRAC 1 cut(s) 630
MnlI CCTC 9 cut(s) 111, 125, 271, 369, 414, 482, 865, 894, 1203
MroNI GCCGGC 2 cut(s) 332, 1139
MseI TTAA 1 cut(s) 1199
MslI CAYNNNNRTG 3 cut(s) 116, 509, 566
MspA1I CMGCKG 3 cut(s) 184, 228, 237
MspI CCGG 4 cut(s) 266, 333, 1104, 1140
MspR9I CCNGG 4 cut(s) 267, 354, 603, 716
MunI CAATTG 1 cut(s) 750
MvaI CCWGG 3 cut(s) 354, 603, 716
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 4 cut(s) 234, 237, 276, 370
NaeI GCCGGC 2 cut(s) 334, 1141
NciI CCSGG 1 cut(s) 267
NdeI CATATG 1 cut(s) 1051
NdeII GATC 4 cut(s) 347, 393, 874, 984
NgoMIV GCCGGC 2 cut(s) 332, 1139
NlaIV GGNNCC 3 cut(s) 814, 1000, 1191
NmuCI GTSAC 1 cut(s) 605
NotI GCGGCCGC 1 cut(s) 232
NspI RCATGY 4 cut(s) 93, 463, 514, 1147
NspV TTCGAA 2 cut(s) 708, 1087
PaeI GCATGC 3 cut(s) 93, 514, 1147
PaeR7I CTCGAG 1 cut(s) 418
PagI TCATGA 1 cut(s) 474
PasI CCCWGGG 1 cut(s) 715
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 2 cut(s) 334, 1141
PfeI GAWTC 4 cut(s) 107, 500, 544, 797
PkrI GCNGC 7 cut(s) 186, 230, 233, 236, 239, 748, 1045
PleI GAGTC 2 cut(s) 204, 602
PpsI GAGTC 2 cut(s) 204, 602
PpuMI RGGWCCY 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 3 cut(s) 352, 601, 714
PspGI CCWGG 3 cut(s) 352, 601, 714
PspN4I GGNNCC 3 cut(s) 814, 1000, 1191
PspPI GGNCC 3 cut(s) 356, 759, 1240
PspPPI RGGWCCY 1 cut(s) 356
PspXI VCTCGAGB 1 cut(s) 418
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 6 cut(s) 250, 297, 416, 588, 1081, 1226
RsaNI GTAC 6 cut(s) 249, 296, 415, 587, 1080, 1225
RseI CAYNNNNRTG 3 cut(s) 116, 509, 566
SacI GAGCTC 1 cut(s) 432
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 1199
SatI GCNGC 7 cut(s) 185, 229, 232, 235, 238, 747, 1044
Sau3AI GATC 4 cut(s) 347, 393, 874, 984
Sau96I GGNCC 3 cut(s) 356, 759, 1240
SchI GAGTC 2 cut(s) 205, 602
ScrFI CCNGG 4 cut(s) 267, 354, 603, 716
SduI GDGCHC 2 cut(s) 375, 432
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 1 cut(s) 450
Sfr274I CTCGAG 1 cut(s) 418
SfuI TTCGAA 2 cut(s) 708, 1087
SinI GGWCC 1 cut(s) 356
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 3 cut(s) 116, 509, 566
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
SpeI ACTAGT 1 cut(s) 145
SphI GCATGC 3 cut(s) 93, 514, 1147
Sse9I AATT 5 cut(s) 124, 750, 789, 1066, 1196
SspMI CTAG 1 cut(s) 146
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 4 cut(s) 265, 352, 601, 714
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 2 cut(s) 372, 959
TaqI TCGA 6 cut(s) 153, 419, 708, 1029, 1087, 1218
TasI AATT 5 cut(s) 124, 750, 789, 1066, 1196
TatI WGTACW 2 cut(s) 586, 1224
TauI GCSGC 2 cut(s) 234, 237
TfiI GAWTC 4 cut(s) 107, 500, 544, 797
Tru1I TTAA 1 cut(s) 1199
Tru9I TTAA 1 cut(s) 1199
TscAI CASTG 2 cut(s) 523, 940
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 5 cut(s) 184, 228, 237, 746, 1043
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 5 cut(s) 681, 699, 773, 860, 1006
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 2 cut(s) 523, 940
VneI GTGCAC 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 356
XagI CCTNNNNNAGG 1 cut(s) 888
XceI RCATGY 4 cut(s) 93, 463, 514, 1147
XcmI CCANNNNNNNNNTGG 2 cut(s) 894, 1090
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.