Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008664

Physical Location & Seq

Genomic Coordinates Reverse (-)

22775236 .. 22778781

Gene Structure 3546 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1212500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGCTGAGAATATTCAATGTTATAAACCTAAATTTAAAAGGCACGAGAGCTGCCTATGGAAATGCTTCAATCAGATTAGAAAATGTAACCAGGAGCTACCGCGTAGATGCCAGGCACTATGAAGAGTTGTCTCTTCTGCTTCAAAGTTCCCAAAGTTCATTAGCATTCAAACCCAATTACCAAGTATTATGTGCCATTCTAAAATCCTGTGCTGCCCTCATAGCCATCAACTTTGGGAAGTCTCTTCATGGTTATGTTGTCAAACAGGGCCATCTTTCCTGCCAGTCTATCTCTAAAGCTCTGCTTAACATGTATGCCAAATGTGGTGTATTGGGTGACTGCAAAATGCTTTTTGGTCAAATAGGCTCCTCGGATCCTGTCATATGGAACATTATCTTATCTGGGTTTTCAGCATCCCGAAATTATGATGCTGAGGTAATAAGACTCTTTCATGAAATGTGTTTAGGTGGGAAGCCTAAGCCCACATCTGTTACCATTGCTACCATTCTTCCTGTGTGTGCTCGCATAGCAGATTTAGATGTGGGGAAGAGCGTGCACTCTTATGTGATAAAGTCTGGATTGATGACAGATGTCTTTGTTGGAAATGCTCTTATATCAATGTATTCAAAATGTGGGCTAGTTTCTGCTGATGCATACGCTGTGTTTAATAGCATTATTGACAAAGATGTTATTTCATGGAATGCAATGATTGCAGGGTTGGCTGAAAATGGATTCATACATGATGCATACAAGTTATTCAGTTGGATGCTCAAAGGACCAGTTGAACCAAATTATGCAACTATTGCAAATGTTCTGCCAATATGTTCTTCTTTAGATAAAAATACAGCGTACTGCGCTGGGAGAGAGATCCATTGTTATGTTCTGCGTCGGAATGAATTGGCAGCAGATGTTACTGTGTTCAATGCTTTGGTAAGTTTTCATTTACAACTTGGACGAATGGAACAAGCGGAATCCTTGTTCCACCGAATGAAATCAAGAGATTTGGTTTCATGGAATGCAATTATTGCAGGGTATGCTTCAAATGGTGAGTGGTCAAAAGCATTGGATCTGTTTCACAAATTACTCTCACTGCAGACAATTGGGCCAGATTCTGTTACCATTGTGAGCATTCTTCCTGCTTGTGCACAGTTACAGAACTTGGAGGTGGGGAAAAAGATCCATGGGTACATTCTTCGACATTGTAGCCTATTTGAAGCTACAACGGTTGGAAATGCCATGGTTAGTTTTTATGCAAAATGCGATAAGCTTGAAGCAGCTTTTGAGACTTTTTTGATGATTTCTAGGAGAGATTTGATATCATGGAATACCATACTTGTTGCCTTTGCAGAGATTGGGTATAGTAACAACTTTTTGAACCTATTAGACGGGATGCTAAGGGAAGATAAGAGTTTATTGCAGAAAAGGAATTTGGTTACTTGCAATTCTATGATTTCAGCTTATGTGAGTTGCGGATCACTGGATGAGGCATATATGATATTTAACAGAATGTCTGAAACAGATCTCACCACTTGGAATCTAATGGTTCGAGCTTATGCTGAAAATGGTTTTTCTGCTGAAGCCCTCAGTCTGTTCCTTGAGTTACAAACTCAAGGAATGAAGCCTGATGCGATGACCATTATGAGCCTCCTTCCCGTGTCTTCTCAAATGTACTCAGTCCACCTGCTGAGACAGTGTCATGGATATGTGATGAGAGCTTGTCTTAGTGATCTGTGCTTGAAAGGAGCTCTGTTGGATATGTACGCAAAATGTGGTGCCATAGTATGTGCTTATAAGCTATTTCAGTCAAGCTTGGATAAGGATCTGGTTATGTTTACAGCTATGGTTGGCGGGTTAGCCATGCATGGAATGGGAGAGGAAGCACTGAGGGTCTTCTCTCACATGCTTGACTTGGGTGTAAAGCCAGATAATGTTGTTATTACTGCTGTTTTATCTGCTTGTAGTCATGCTGGCCTGGTGAATGAAGGGTTGAAGATATTTAATTCGATAGAAGAGGTTCATGGGGTCAAACCAACAATGGAACAGTATGCTTGTGTGGTGGATCTCCTTGCGAGAGGTGGACAAATTGAGGATGCATTTTCTTTTGTGTCCAGGATGCCTATTGAAGCTAATGCTAGCATATGGGGAACACTGCTGGGTGCTTGCAGGACTCATCGTAAGGTGGAATTGGGCCGTGTTGCGGCAGATCATCTTTTTAAAAATGAAGCTAATAATACTGGGAACTACGTGGTGATGTCAAATCTATATGCAGCAGAAGCTAGATGGGATGGGGTCATGGAGATAAGAAAGCTGATGAAAACACGAGATCTAACGAAGCCAGCAGGATGCAGCTGGATAGAGGTGGAGAGGAGGAAGAATCTTTTTATAGCTGGAGACTGGTCTCATCCAGAAAGAAGTGTTATTTACAGCACATTAAGTGCATTGGACCAACAAATAAAAGAGCCAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009451 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010035 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0033993 GO:0034641 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:1900864 GO:1901360 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

823

Amino Acids

90.86

Weight (kDa)

7.71

Isoelectric Point (pI)

40.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	228 - 267	1.5e-08	PPR repeat family
PPR_2	PF13041	334 - 382	4.5e-07	PPR repeat family
PPR	PF01535	336 - 363	4e-06	PPR repeat
PPR	PF01535	478 - 505	1.1e-06	PPR repeat
PPR_2	PF13041	607 - 654	2.4e-07	PPR repeat family
E_motif	PF20431	724 - 786	1.4e-16	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008664)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08490 AT5G08490 AT5G08490 AT5G08490 AT5G08490 AT5G08490 AT5G08490 AT5G08490
fragaria_vesca	FvH4_1g29890 FvH4_1g29890 FvH4_1g29890
malus_domestica	MD02G1212500.v1.1
prunus_persica	Prupe.3G127300_v2.0.a1
pyrus_communis	pycom02g17620
rosa_laevigata	RLG00000022567
rosa_multiflora	Rmu_sc0005781.1_g000010
rosa_roxburghii	Rroxscaffold_6G00389610
rosa_rugosa	Rorug03G0279300
rosa_samantha	Rh3AG326200 Rh3BG362500 Rh3CG358600 Rh3DG361900
rosa_wichuraiana	Rw3G028560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	23, 1791
AarI	CACCTGC	1 cut(s)	1689
Acc36I	ACCTGC	1 cut(s)	1689
AccB1I	GGYRCC	1 cut(s)	1772
AccII	CGCG	1 cut(s)	102
AciI	CCGC	5 cut(s)	100, 968, 1470, 1848, 2198
AclWI	GGATC	8 cut(s)	368, 381, 862, 1074, 1171, 1480, 1827, 2067
AcsI	RAATTY	2 cut(s)	31, 1426
AcuI	CTGAAG	1 cut(s)	1596
AfaI	GTAC	4 cut(s)	851, 1187, 1670, 1760
AfiI	CCNNNNNNNGG	1 cut(s)	2197
AflIII	ACRYGT	1 cut(s)	309
AjnI	CCWGG	4 cut(s)	89, 110, 1971, 2108
AjuI	GAANNNNNNNTTGG	4 cut(s)	811, 843, 1412, 1444
AloI	GAACNNNNNNTCC	2 cut(s)	962, 994
Alw21I	GWGCWC	4 cut(s)	523, 558, 1147, 1747
Alw26I	GTCTC	6 cut(s)	135, 246, 1277, 1681, 2385, 2403
Alw44I	GTGCAC	2 cut(s)	554, 1143
AlwI	GGATC	8 cut(s)	368, 381, 862, 1074, 1171, 1480, 1827, 2067
AlwNI	CAGNNNCTG	1 cut(s)	1112
AoxI	GGCC	4 cut(s)	268, 1103, 1969, 2188
ApaLI	GTGCAC	2 cut(s)	554, 1143
ApeKI	GCWGC	6 cut(s)	50, 212, 902, 1274, 2267, 2346
ApoI	RAATTY	2 cut(s)	31, 1426
Asp700I	GAANNNNTTC	2 cut(s)	64, 2013
AspLEI	GCGC	1 cut(s)	857
AspS9I	GGNCC	5 cut(s)	268, 776, 1103, 2188, 2443
AsuHPI	GGTGA	5 cut(s)	347, 1058, 1516, 1987, 2260
AsuNHI	GCTAGC	1 cut(s)	2132
AvaII	GGWCC	2 cut(s)	776, 2443
BaeGI	GKGCMC	2 cut(s)	558, 1147
BamHI	GGATCC	1 cut(s)	373
BanI	GGYRCC	1 cut(s)	1772
BanII	GRGCYC	1 cut(s)	1747
BauI	CACGAG	2 cut(s)	43, 2319
BbsI	GAAGAC	2 cut(s)	1650, 1882
Bbv12I	GWGCWC	4 cut(s)	523, 558, 1147, 1747
BbvCI	CCTCAGC	1 cut(s)	432
BbvI	GCAGC	6 cut(s)	37, 199, 914, 1286, 2279, 2358
BccI	CCATC	4 cut(s)	233, 279, 2274, 2279
BceAI	ACGGC	1 cut(s)	2175
BciT130I	CCWGG	4 cut(s)	91, 112, 1973, 2110
BcoDI	GTCTC	6 cut(s)	135, 246, 1277, 1681, 2385, 2403
BfaI	CTAG	4 cut(s)	638, 1302, 2133, 2277
BfmI	CTRYAG	1 cut(s)	1091
BfuAI	ACCTGC	1 cut(s)	1689
BglII	AGATCT	2 cut(s)	1519, 2323
BisI	GCNGC	7 cut(s)	51, 213, 903, 1275, 2199, 2268, 2347
BlsI	GCNGC	7 cut(s)	52, 214, 904, 1276, 2200, 2269, 2348
Bme1390I	CCNGG	4 cut(s)	91, 112, 1973, 2110
Bme18I	GGWCC	2 cut(s)	776, 2443
BmgT120I	GGNCC	5 cut(s)	268, 776, 1103, 2188, 2443
BmiI	GGNNCC	3 cut(s)	367, 375, 1774
BmrFI	CCNGG	4 cut(s)	91, 112, 1973, 2110
BmrI	ACTGGG	1 cut(s)	2244
BmtI	GCTAGC	1 cut(s)	2136
BmuI	ACTGGG	1 cut(s)	2244
BoxI	GACNNNNGTC	1 cut(s)	590
BpiI	GAAGAC	2 cut(s)	1650, 1882
BpmI	CTGGAG	1 cut(s)	2409
Bpu10I	CCTNAGC	3 cut(s)	432, 477, 1394
BpuEI	CTTGAG	2 cut(s)	1593, 1616
BsaAI	YACGTR	1 cut(s)	2245
BsaBI	GATNNNNATC	1 cut(s)	1818
BsaI	GGTCTC	1 cut(s)	2403
BsaJI	CCNNGG	3 cut(s)	369, 1180, 1236
Bsc4I	CCNNNNNNNGG	1 cut(s)	2197
Bse1I	ACTGG	6 cut(s)	283, 779, 1482, 2239, 2399, 2462
Bse3DI	GCAATG	2 cut(s)	495, 711
Bse8I	GATNNNNATC	1 cut(s)	1818
BseBI	CCWGG	4 cut(s)	91, 112, 1973, 2110
BseDI	CCNNGG	3 cut(s)	369, 1180, 1236
BseGI	GGATG	9 cut(s)	413, 771, 1395, 1486, 2095, 2118, 2290, 2348, 2401
BseJI	GATNNNNATC	1 cut(s)	1818
BseLI	CCNNNNNNNGG	1 cut(s)	2197
BseMI	GCAATG	2 cut(s)	495, 711
BseMII	CTCAG	5 cut(s)	423, 1597, 1676, 1686, 1874
BseNI	ACTGG	6 cut(s)	283, 779, 1482, 2239, 2399, 2462
BseRI	GAGGAG	2 cut(s)	358, 2380
BseSI	GKGCMC	2 cut(s)	558, 1147
BseXI	GCAGC	6 cut(s)	37, 199, 914, 1286, 2279, 2358
BseYI	CCCAGC	2 cut(s)	857, 2152
Bsh1236I	CGCG	1 cut(s)	102
BshFI	GGCC	4 cut(s)	270, 1105, 1971, 2190
BshNI	GGYRCC	1 cut(s)	1772
BsiHKAI	GWGCWC	4 cut(s)	523, 558, 1147, 1747
BslI	CCNNNNNNNGG	1 cut(s)	2197
BsmAI	GTCTC	6 cut(s)	135, 246, 1277, 1681, 2385, 2403
BsmI	GAATGC	4 cut(s)	164, 706, 1021, 1128
BsnI	GGCC	4 cut(s)	270, 1105, 1971, 2190
Bso31I	GGTCTC	1 cut(s)	2403
Bsp1286I	GDGCHC	4 cut(s)	523, 558, 1147, 1747
Bsp19I	CCATGG	2 cut(s)	1180, 1236
BspACI	CCGC	5 cut(s)	100, 968, 1470, 1848, 2198
BspANI	GGCC	4 cut(s)	270, 1105, 1971, 2190
BspCNI	CTCAG	5 cut(s)	424, 1596, 1677, 1685, 1875
BspFNI	CGCG	1 cut(s)	102
BspHI	TCATGA	1 cut(s)	451
BspLI	GGNNCC	3 cut(s)	367, 375, 1774
BspMAI	CTGCAG	1 cut(s)	1095
BspMI	ACCTGC	1 cut(s)	1689
BspOI	GCTAGC	1 cut(s)	2136
BspPI	GGATC	8 cut(s)	368, 381, 862, 1074, 1171, 1480, 1827, 2067
BspQI	GCTCTTC	1 cut(s)	542
BspT107I	GGYRCC	1 cut(s)	1772
BspTNI	GGTCTC	1 cut(s)	2403
BsrDI	GCAATG	2 cut(s)	495, 711
BsrI	ACTGG	6 cut(s)	283, 779, 1482, 2239, 2399, 2462
BssECI	CCNNGG	3 cut(s)	369, 1180, 1236
BssSI	CACGAG	2 cut(s)	43, 2319
BssT1I	CCWWGG	2 cut(s)	1180, 1236
Bst2BI	CACGAG	2 cut(s)	43, 2319
Bst2UI	CCWGG	4 cut(s)	91, 112, 1973, 2110
Bst4CI	ACNGT	5 cut(s)	916, 1149, 1225, 1692, 2043
Bst6I	CTCTTC	5 cut(s)	117, 138, 249, 542, 2004
BstAPI	GCANNNNNTGC	4 cut(s)	710, 803, 1025, 1034
BstBAI	YACGTR	1 cut(s)	2245
BstC8I	GCNNGC	6 cut(s)	523, 554, 1969, 2134, 2161, 2337
BstDEI	CTNAG	9 cut(s)	5, 432, 477, 1394, 1583, 1672, 1685, 1721, 1883
BstDSI	CCRYGG	2 cut(s)	1180, 1236
BstF5I	GGATG	9 cut(s)	413, 771, 1395, 1486, 2095, 2118, 2290, 2348, 2401
BstFNI	CGCG	1 cut(s)	102
BstHHI	GCGC	1 cut(s)	857
BstMAI	GTCTC	6 cut(s)	135, 246, 1277, 1681, 2385, 2403
BstMWI	GCNNNNNNNGC	9 cut(s)	221, 527, 710, 719, 803, 854, 1025, 1034, 2273
BstNI	CCWGG	4 cut(s)	91, 112, 1973, 2110
BstNSI	RCATGY	2 cut(s)	313, 1903
BstPAI	GACNNNNGTC	1 cut(s)	590
BstSCI	CCNGG	4 cut(s)	89, 110, 1971, 2108
BstSFI	CTRYAG	1 cut(s)	1091
BstSLI	GKGCMC	2 cut(s)	558, 1147
BstUI	CGCG	1 cut(s)	102
BstV1I	GCAGC	6 cut(s)	37, 199, 914, 1286, 2279, 2358
BstV2I	GAAGAC	2 cut(s)	1650, 1882
BstX2I	RGATCY	8 cut(s)	373, 867, 1066, 1176, 1519, 1819, 2059, 2323
BstYI	RGATCY	8 cut(s)	373, 867, 1066, 1176, 1519, 1819, 2059, 2323
BsuRI	GGCC	4 cut(s)	270, 1105, 1971, 2190
BtgI	CCRYGG	2 cut(s)	1180, 1236
BtgZI	GCGATG	1 cut(s)	1643
BtsCI	GGATG	9 cut(s)	413, 771, 1395, 1486, 2095, 2118, 2290, 2348, 2401
BtsI	GCAGTG	2 cut(s)	1088, 2147
BtsIMutI	CAGTG	5 cut(s)	1088, 1475, 1697, 1880, 2147
BveI	ACCTGC	1 cut(s)	1689
Cac8I	GCNNGC	6 cut(s)	523, 554, 1969, 2134, 2161, 2337
CaiI	CAGNNNCTG	1 cut(s)	1112
CciI	TCATGA	1 cut(s)	451
CfoI	GCGC	1 cut(s)	857
Cfr13I	GGNCC	5 cut(s)	268, 776, 1103, 2188, 2443
CseI	GACGC	1 cut(s)	875
Csp6I	GTAC	4 cut(s)	850, 1186, 1669, 1759
CviQI	GTAC	4 cut(s)	850, 1186, 1669, 1759
DdeI	CTNAG	9 cut(s)	5, 432, 477, 1394, 1583, 1672, 1685, 1721, 1883
DraI	TTTAAA	2 cut(s)	36, 2215
Eam1104I	CTCTTC	5 cut(s)	117, 138, 249, 542, 2004
EarI	CTCTTC	5 cut(s)	117, 138, 249, 542, 2004
Ecl136II	GAGCTC	1 cut(s)	1745
Eco130I	CCWWGG	2 cut(s)	1180, 1236
Eco24I	GRGCYC	1 cut(s)	1747
Eco31I	GGTCTC	1 cut(s)	2403
Eco32I	GATATC	1 cut(s)	1317
Eco47I	GGWCC	2 cut(s)	776, 2443
Eco53kI	GAGCTC	1 cut(s)	1745
Eco57I	CTGAAG	1 cut(s)	1596
EcoICRI	GAGCTC	1 cut(s)	1745
EcoRII	CCWGG	4 cut(s)	89, 110, 1971, 2108
EcoRV	GATATC	1 cut(s)	1317
EcoT14I	CCWWGG	2 cut(s)	1180, 1236
EcoT22I	ATGCAT	4 cut(s)	655, 748, 1863, 2095
EcoT38I	GRGCYC	1 cut(s)	1747
ErhI	CCWWGG	2 cut(s)	1180, 1236
FalI	AAGNNNNNCTT	2 cut(s)	288, 320
FauI	CCCGC	1 cut(s)	1841
FauNDI	CATATG	2 cut(s)	383, 2138
Fnu4HI	GCNGC	7 cut(s)	51, 213, 903, 1275, 2199, 2268, 2347
FokI	GGATG	9 cut(s)	400, 778, 1402, 1493, 2102, 2125, 2297, 2355, 2388
FriOI	GRGCYC	1 cut(s)	1747
Fsp4HI	GCNGC	7 cut(s)	51, 213, 903, 1275, 2199, 2268, 2347
FspBI	CTAG	4 cut(s)	638, 1302, 2133, 2277
GlaI	GCGC	1 cut(s)	856
GluI	GCNGC	7 cut(s)	51, 213, 903, 1275, 2199, 2268, 2347
GsaI	CCCAGC	2 cut(s)	861, 2156
GsuI	CTGGAG	1 cut(s)	2409
HaeIII	GGCC	4 cut(s)	270, 1105, 1971, 2190
HgaI	GACGC	1 cut(s)	875
HhaI	GCGC	1 cut(s)	857
Hin6I	GCGC	1 cut(s)	855
HinP1I	GCGC	1 cut(s)	855
HindIII	AAGCTT	2 cut(s)	1265, 1807
HinfI	GANTC	7 cut(s)	444, 732, 971, 1109, 1534, 2167, 2374
HphI	GGTGA	5 cut(s)	347, 1058, 1516, 1987, 2260
Hpy166II	GTNNAC	5 cut(s)	556, 1145, 1678, 1833, 2078
Hpy188I	TCNGA	4 cut(s)	74, 373, 891, 1513
Hpy188III	TCNNGA	5 cut(s)	417, 452, 576, 996, 2405
Hpy8I	GTNNAC	5 cut(s)	556, 1145, 1678, 1833, 2078
Hpy99I	CGWCG	1 cut(s)	891
HpyAV	CCTTC	2 cut(s)	1658, 1976
HpyCH4III	ACNGT	5 cut(s)	916, 1149, 1225, 1692, 2043
HpyCH4IV	ACGT	1 cut(s)	2244
HpyF10VI	GCNNNNNNNGC	9 cut(s)	221, 527, 710, 719, 803, 854, 1025, 1034, 2273
HpyF3I	CTNAG	9 cut(s)	5, 432, 477, 1394, 1583, 1672, 1685, 1721, 1883
HpySE526I	ACGT	1 cut(s)	2244
HspAI	GCGC	1 cut(s)	855
LguI	GCTCTTC	1 cut(s)	542
LmnI	GCTCC	3 cut(s)	93, 371, 1742
Lsp1109I	GCAGC	6 cut(s)	37, 199, 914, 1286, 2279, 2358
MaeI	CTAG	4 cut(s)	638, 1302, 2133, 2277
MaeII	ACGT	1 cut(s)	2244
MaeIII	GTNAC	9 cut(s)	85, 335, 490, 910, 1114, 1149, 1361, 1432, 1599
MfeI	CAATTG	1 cut(s)	1098
MflI	RGATCY	8 cut(s)	373, 867, 1066, 1176, 1519, 1819, 2059, 2323
MhlI	GDGCHC	4 cut(s)	523, 558, 1147, 1747
MlyI	GAGTC	2 cut(s)	438, 2161
MmeI	TCCRAC	5 cut(s)	580, 743, 869, 1207, 1731
Mph1103I	ATGCAT	4 cut(s)	655, 748, 1863, 2095
MroXI	GAANNNNTTC	2 cut(s)	64, 2013
MseI	TTAA	8 cut(s)	35, 306, 666, 1500, 1998, 2214, 2432, 2467
MslI	CAYNNNNRTG	4 cut(s)	252, 561, 876, 1701
MspA1I	CMGCKG	1 cut(s)	2349
MspR9I	CCNGG	4 cut(s)	91, 112, 1973, 2110
MunI	CAATTG	1 cut(s)	1098
Mva1269I	GAATGC	4 cut(s)	164, 706, 1021, 1128
MvaI	CCWGG	4 cut(s)	91, 112, 1973, 2110
MvnI	CGCG	1 cut(s)	102
MwoI	GCNNNNNNNGC	9 cut(s)	221, 527, 710, 719, 803, 854, 1025, 1034, 2273
NcoI	CCATGG	2 cut(s)	1180, 1236
NdeI	CATATG	2 cut(s)	383, 2138
NheI	GCTAGC	1 cut(s)	2132
NlaIV	GGNNCC	3 cut(s)	367, 375, 1774
NmuCI	GTSAC	1 cut(s)	335
NsiI	ATGCAT	4 cut(s)	655, 748, 1863, 2095
NspI	RCATGY	2 cut(s)	313, 1903
PagI	TCATGA	1 cut(s)	451
PaqCI	CACCTGC	1 cut(s)	1689
PciI	ACATGT	1 cut(s)	309
PciSI	GCTCTTC	1 cut(s)	542
PctI	GAATGC	4 cut(s)	164, 706, 1021, 1128
PdmI	GAANNNNTTC	2 cut(s)	64, 2013
PfeI	GAWTC	5 cut(s)	732, 971, 1109, 1534, 2374
PflFI	GACNNNGTC	1 cut(s)	1692
PfoI	TCCNGGA	1 cut(s)	2108
PkrI	GCNGC	7 cut(s)	52, 214, 904, 1276, 2200, 2269, 2348
PleI	GAGTC	2 cut(s)	438, 2161
PpsI	GAGTC	2 cut(s)	438, 2161
Ppu21I	YACGTR	1 cut(s)	2245
PscI	ACATGT	1 cut(s)	309
PshAI	GACNNNNGTC	1 cut(s)	590
PsiI	TTATAA	2 cut(s)	23, 1791
Psp124BI	GAGCTC	1 cut(s)	1747
Psp6I	CCWGG	4 cut(s)	89, 110, 1971, 2108
PspFI	CCCAGC	2 cut(s)	857, 2152
PspGI	CCWGG	4 cut(s)	89, 110, 1971, 2108
PspN4I	GGNNCC	3 cut(s)	367, 375, 1774
PspPI	GGNCC	5 cut(s)	268, 776, 1103, 2188, 2443
PstI	CTGCAG	1 cut(s)	1095
PstNI	CAGNNNCTG	1 cut(s)	1112
PsuI	RGATCY	8 cut(s)	373, 867, 1066, 1176, 1519, 1819, 2059, 2323
PsyI	GACNNNGTC	1 cut(s)	1692
PvuII	CAGCTG	1 cut(s)	2349
RsaI	GTAC	4 cut(s)	851, 1187, 1670, 1760
RsaNI	GTAC	4 cut(s)	850, 1186, 1669, 1759
RseI	CAYNNNNRTG	4 cut(s)	252, 561, 876, 1701
SacI	GAGCTC	1 cut(s)	1747
SapI	GCTCTTC	1 cut(s)	542
SaqAI	TTAA	8 cut(s)	35, 306, 666, 1500, 1998, 2214, 2432, 2467
SatI	GCNGC	7 cut(s)	51, 213, 903, 1275, 2199, 2268, 2347
Sau96I	GGNCC	5 cut(s)	268, 776, 1103, 2188, 2443
SchI	GAGTC	2 cut(s)	438, 2161
ScrFI	CCNGG	4 cut(s)	91, 112, 1973, 2110
SduI	GDGCHC	4 cut(s)	523, 558, 1147, 1747
SfcI	CTRYAG	1 cut(s)	1091
SinI	GGWCC	2 cut(s)	776, 2443
SmiMI	CAYNNNNRTG	4 cut(s)	252, 561, 876, 1701
SmlI	CTYRAG	2 cut(s)	1595, 1608
SmoI	CTYRAG	2 cut(s)	1595, 1608
SsiI	CCGC	5 cut(s)	100, 968, 1470, 1848, 2198
SspI	AATATT	1 cut(s)	12
SspMI	CTAG	4 cut(s)	638, 1302, 2133, 2277
SstI	GAGCTC	1 cut(s)	1747
StyD4I	CCNGG	4 cut(s)	89, 110, 1971, 2108
StyI	CCWWGG	2 cut(s)	1180, 1236
TaaI	ACNGT	5 cut(s)	916, 1149, 1225, 1692, 2043
TaiI	ACGT	1 cut(s)	2247
TaqI	TCGA	3 cut(s)	1195, 1546, 2003
TatI	WGTACW	1 cut(s)	1668
TauI	GCSGC	1 cut(s)	2201
TfiI	GAWTC	5 cut(s)	732, 971, 1109, 1534, 2374
Tru1I	TTAA	8 cut(s)	35, 306, 666, 1500, 1998, 2214, 2432, 2467
Tru9I	TTAA	8 cut(s)	35, 306, 666, 1500, 1998, 2214, 2432, 2467
TscAI	CASTG	5 cut(s)	1095, 1482, 1697, 1887, 2154
TseFI	GTSAC	1 cut(s)	335
TseI	GCWGC	6 cut(s)	50, 212, 902, 1274, 2267, 2346
Tsp45I	GTSAC	1 cut(s)	335
TspRI	CASTG	5 cut(s)	1095, 1482, 1697, 1887, 2154
Tth111I	GACNNNGTC	1 cut(s)	1692
VneI	GTGCAC	2 cut(s)	554, 1143
VpaK11BI	GGWCC	2 cut(s)	776, 2443
XapI	RAATTY	2 cut(s)	31, 1426
XceI	RCATGY	2 cut(s)	313, 1903
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1864
XmnI	GAANNNNTTC	2 cut(s)	64, 2013
XspI	CTAG	4 cut(s)	638, 1302, 2133, 2277
Zsp2I	ATGCAT	4 cut(s)	655, 748, 1863, 2095

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.