Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008337

Physical Location & Seq

Genomic Coordinates Forward (+)

29349492 .. 29355130

Gene Structure 5639 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1243100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGGCAGTAGCTGAGCTATCAAAACCTTCATACACCAAGACCCGCCTTTTTCAGTCTTCCCAGCTCTCATCCTTATCGACCCATCTCCACTCTCACAGCCGTCTCACTTTTCGGCCCCTCCATCACACTCGAAATTCCAGAATCTGTTGCTCTGTTGCACCCAATCAAGTTCAAGCTCCAGCTGCTCCAGAAAAAACCCAAGACCCGAAGAGCAAGGCAGAGTGCTTCGGCGTGTTCTGCCTCACCTATGATCTCATAGCTGAAGAAGAGACAAAATCATGGAAGAAATTAATTAATATTGCAGTCTCAGGTGCAGCTGGGATGATATCTAATCATCTACTCTTTAAACTAGCGTCAGGCGAGGTTTTTGGGCCAGACCAACCTATTGCATTGAAACTATTGGGATCTGAAAGGTCCCTCCAAGCTCTTGAAGGAGTTGCTATGGAATTGGAGGATTCCCTATTTCCGTTGCTGCGGGAGGTGAGCATTGGCATTGATCCATATGAGGTTTTCCAAGATGCAGAATGGGCTCTTTTAATAGGAGCGAAGCCTCGAGGTCCTGGAATGGAAAGAGCAGGCTTGTTGGATCTGAATGGGCAGATTTTCGTCGAGCAGGGAAAAGCTCTTAATGCTGTTGCATCACGTAATGTCAAAGTGATAGTAGTTGGAAACCCTTGTAACACAAATGCACTCATCTGTTTGAAAAATGCTCCAAACATACCTGCAAAGAATTTTCATGCTTTGACACGGCTCGATGAAAATAGAGCAAAATGTCAGCTAGCCCTCAAAGCAGGCGTCTTCTATGATAAAGTGTCAAACGTGACCATCTGGGGAAACCACTCAACCACCCAGGTGCCGGACTTCTTAAATGCTACAATTAATGGCTTGCCTGTCAAAGAGGTCATCAAGGATCACAAATGGTTAGAGGAAGAGTTTACCGAAAGTATCCAGAAGAGAGGAGGGGTGCTTATAAAAAAATGGGGAAGATCGTCAGCTGCATCAACTGCCGTGTCAATTGCAGATGCCATGAAGTCTTTGGTTACTCCTACCCCTGAGGGCGATTGGTTTTCATCTGCAGTTTACACAAATGGAAATCCCTACGGTATAGCAGAGGATTTAGTGTTCAGCATGCCATGCAGATCTAAAGGAGACGGCGACTATGAACTTGTGAAGGACATACAATTTGATGACTATCTTCGCAAGCGAATAGCAAAGAGCGAAGCTGAGTTACTAGCTGAGAAAAGATGCGTCGCTCACCTTACCGGGCAGGGTATCGCTGTGTGTGATTTACCCGAGGATACTATGCTTCCTGGAGAGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006107 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008746 GO:0009060 GO:0009117 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0016651 GO:0016652 GO:0016999 GO:0017144 GO:0019362 GO:0019637 GO:0019674 GO:0019752 GO:0030060 GO:0031967 GO:0031975 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046483 GO:0046496 GO:0048046 GO:0050896 GO:0051186 GO:0051775 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072524 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

441

Amino Acids

48.15

Weight (kDa)

6.39

Isoelectric Point (pI)

31.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	99 - 246	2.5e-33	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	249 - 419	1.4e-48	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58330 AT5G58330 AT5G58330
fragaria_vesca	FvH4_7g06530
malus_domestica	MD02G1243100.v1.1 MD07G1073300.v1.1
prunus_persica	Prupe.2G091600_v2.0.a1
pyrus_communis	pycom02g20970 pycom07g05680
rosa_chinensis	RchiOBHm_Chr1g0334961
rosa_laevigata	RLG00000029409
rosa_multiflora	Rmu_co7973608.1_g000002 Rmu_sc0000574.1_g000062 Rmu_sc0000574.1_g000065
rosa_roxburghii	Rroxscaffold_4G00316350
rosa_rugosa	Rorug01G0118000 Rorug01G0118100
rosa_samantha	Rh1AG141400 Rh1BG108900 Rh1CG133300 Rh1DG145900 Rh1DG146000
rosa_wichuraiana	Rw1G011730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	971
AbsI	CCTCGAGG	1 cut(s)	552
Acc36I	ACCTGC	1 cut(s)	730
AccB1I	GGYRCC	1 cut(s)	853
AciI	CCGC	2 cut(s)	43, 475
AclWI	GGATC	4 cut(s)	412, 491, 594, 918
AcsI	RAATTY	2 cut(s)	133, 730
AcuI	CTGAAG	1 cut(s)	282
AcyI	GRCGYC	1 cut(s)	795
AfiI	CCNNNNNNNGG	1 cut(s)	856
AgsI	TTSAA	4 cut(s)	173, 394, 431, 703
AjnI	CCWGG	3 cut(s)	559, 849, 1309
AleI	CACNNNNGTG	1 cut(s)	851
AloI	GAACNNNNNNTCC	2 cut(s)	1106, 1138
Alw26I	GTCTC	4 cut(s)	107, 263, 310, 1143
AlwI	GGATC	4 cut(s)	412, 491, 594, 918
AlwNI	CAGNNNCTG	2 cut(s)	11, 144
Ama87I	CYCGRG	2 cut(s)	552, 1292
AoxI	GGCC	2 cut(s)	113, 371
ApeKI	GCWGC	4 cut(s)	182, 314, 472, 995
ApoI	RAATTY	2 cut(s)	133, 730
ArsI	GACNNNNNNTTYG	4 cut(s)	780, 812, 1166, 1198
AseI	ATTAAT	3 cut(s)	290, 294, 879
AspS9I	GGNCC	4 cut(s)	114, 371, 414, 557
AsuC2I	CCSGG	1 cut(s)	1264
AsuHPI	GGTGA	3 cut(s)	235, 493, 1247
AsuNHI	GCTAGC	1 cut(s)	778
AvaI	CYCGRG	2 cut(s)	552, 1292
AvaII	GGWCC	2 cut(s)	414, 557
AxyI	CCTNAGG	1 cut(s)	1053
BanI	GGYRCC	1 cut(s)	853
BanII	GRGCYC	1 cut(s)	532
BarI	GAAGNNNNNNTAC	2 cut(s)	1212, 1244
BbsI	GAAGAC	2 cut(s)	48, 790
BbvI	GCAGC	4 cut(s)	169, 326, 459, 982
BccI	CCATC	3 cut(s)	90, 129, 833
BceAI	ACGGC	4 cut(s)	84, 764, 992, 1168
BciT130I	CCWGG	3 cut(s)	561, 851, 1311
BciVI	GTATCC	2 cut(s)	956, 1291
BcnI	CCSGG	1 cut(s)	1264
BcoDI	GTCTC	4 cut(s)	107, 263, 310, 1143
BfaI	CTAG	3 cut(s)	350, 779, 1232
BfmI	CTRYAG	1 cut(s)	1074
BfuAI	ACCTGC	1 cut(s)	730
BfuI	GTATCC	2 cut(s)	956, 1291
BglII	AGATCT	1 cut(s)	1139
BisI	GCNGC	4 cut(s)	183, 315, 473, 996
BlpI	GCTNAGC	1 cut(s)	12
BlsI	GCNGC	4 cut(s)	184, 316, 474, 997
Bme1390I	CCNGG	4 cut(s)	561, 851, 1264, 1311
Bme18I	GGWCC	2 cut(s)	414, 557
BmeT110I	CYCGRG	2 cut(s)	552, 1292
BmgT120I	GGNCC	4 cut(s)	114, 371, 414, 557
BmiI	GGNNCC	3 cut(s)	116, 416, 855
BmrFI	CCNGG	4 cut(s)	561, 851, 1264, 1311
BmsI	GCATC	5 cut(s)	508, 647, 1007, 1012, 1235
BmtI	GCTAGC	1 cut(s)	782
BpiI	GAAGAC	2 cut(s)	48, 790
BpmI	CTGGAG	2 cut(s)	162, 171
Bpu1102I	GCTNAGC	1 cut(s)	12
BpuMI	CCSGG	1 cut(s)	1264
BsaAI	YACGTR	1 cut(s)	644
BsaBI	GATNNNNATC	1 cut(s)	1191
BsaHI	GRCGYC	1 cut(s)	795
BsaJI	CCNNGG	2 cut(s)	849, 1293
Bsc4I	CCNNNNNNNGG	1 cut(s)	856
Bse21I	CCTNAGG	1 cut(s)	1053
Bse8I	GATNNNNATC	1 cut(s)	1191
BseBI	CCWGG	3 cut(s)	561, 851, 1311
BseDI	CCNNGG	2 cut(s)	849, 1293
BseGI	GGATG	2 cut(s)	68, 327
BseJI	GATNNNNATC	1 cut(s)	1191
BseLI	CCNNNNNNNGG	1 cut(s)	856
BseMII	CTCAG	4 cut(s)	321, 1044, 1215, 1227
BseRI	GAGGAG	1 cut(s)	972
BseXI	GCAGC	4 cut(s)	169, 326, 459, 982
BseYI	CCCAGC	2 cut(s)	60, 317
BsgI	GTGCAG	1 cut(s)	333
BshFI	GGCC	2 cut(s)	115, 373
BshNI	GGYRCC	1 cut(s)	853
BsiHKCI	CYCGRG	2 cut(s)	552, 1292
BsiSI	CCGG	2 cut(s)	857, 1263
BslFI	GGGAC	1 cut(s)	400
BslI	CCNNNNNNNGG	1 cut(s)	856
BsmAI	GTCTC	4 cut(s)	107, 263, 310, 1143
BsmBI	CGTCTC	2 cut(s)	107, 1143
BsmFI	GGGAC	1 cut(s)	400
BsnI	GGCC	2 cut(s)	115, 373
BsoBI	CYCGRG	2 cut(s)	552, 1292
Bsp1286I	GDGCHC	1 cut(s)	532
Bsp143I	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
Bsp1720I	GCTNAGC	1 cut(s)	12
BspACI	CCGC	2 cut(s)	43, 475
BspANI	GGCC	2 cut(s)	115, 373
BspCNI	CTCAG	5 cut(s)	4, 320, 1045, 1216, 1228
BspLI	GGNNCC	3 cut(s)	116, 416, 855
BspMAI	CTGCAG	1 cut(s)	1078
BspMI	ACCTGC	1 cut(s)	730
BspOI	GCTAGC	1 cut(s)	782
BspPI	GGATC	4 cut(s)	412, 491, 594, 918
BspQI	GCTCTTC	1 cut(s)	203
BspT107I	GGYRCC	1 cut(s)	853
BssECI	CCNNGG	2 cut(s)	849, 1293
BssMI	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
BssNI	GRCGYC	1 cut(s)	795
Bst2UI	CCWGG	3 cut(s)	561, 851, 1311
Bst4CI	ACNGT	1 cut(s)	1103
Bst6I	CTCTTC	4 cut(s)	203, 261, 924, 947
BstACI	GRCGYC	1 cut(s)	795
BstAPI	GCANNNNNTGC	2 cut(s)	1004, 1134
BstBAI	YACGTR	1 cut(s)	644
BstC8I	GCNNGC	6 cut(s)	577, 780, 793, 887, 1130, 1202
BstDEI	CTNAG	5 cut(s)	12, 307, 1053, 1224, 1236
BstF5I	GGATG	2 cut(s)	68, 327
BstKTI	GATC	7 cut(s)	253, 407, 499, 589, 913, 989, 1142
BstMAI	GTCTC	4 cut(s)	107, 263, 310, 1143
BstMBI	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
BstMWI	GCNNNNNNNGC	7 cut(s)	182, 237, 527, 629, 788, 1004, 1134
BstNI	CCWGG	3 cut(s)	561, 851, 1311
BstNSI	RCATGY	1 cut(s)	1132
BstSCI	CCNGG	4 cut(s)	559, 849, 1262, 1309
BstSFI	CTRYAG	1 cut(s)	1074
BstV1I	GCAGC	4 cut(s)	169, 326, 459, 982
BstV2I	GAAGAC	2 cut(s)	48, 790
BstX2I	RGATCY	3 cut(s)	404, 586, 1139
BstYI	RGATCY	3 cut(s)	404, 586, 1139
Bsu36I	CCTNAGG	1 cut(s)	1053
BsuI	GTATCC	2 cut(s)	956, 1291
BsuRI	GGCC	2 cut(s)	115, 373
BtsCI	GGATG	2 cut(s)	68, 327
BveI	ACCTGC	1 cut(s)	730
Cac8I	GCNNGC	6 cut(s)	577, 780, 793, 887, 1130, 1202
CaiI	CAGNNNCTG	2 cut(s)	11, 144
Cfr13I	GGNCC	4 cut(s)	114, 371, 414, 557
CseI	GACGC	3 cut(s)	342, 784, 1237
CviAII	CATG	5 cut(s)	279, 737, 1027, 1129, 1134
DdeI	CTNAG	5 cut(s)	12, 307, 1053, 1224, 1236
DpnI	GATC	7 cut(s)	252, 406, 498, 588, 912, 988, 1141
DpnII	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
DraI	TTTAAA	1 cut(s)	346
Eam1104I	CTCTTC	4 cut(s)	203, 261, 924, 947
EarI	CTCTTC	4 cut(s)	203, 261, 924, 947
Eco24I	GRGCYC	1 cut(s)	532
Eco32I	GATATC	1 cut(s)	327
Eco47I	GGWCC	2 cut(s)	414, 557
Eco57I	CTGAAG	1 cut(s)	282
Eco81I	CCTNAGG	1 cut(s)	1053
Eco88I	CYCGRG	2 cut(s)	552, 1292
EcoO109I	RGGNCCY	2 cut(s)	414, 557
EcoRII	CCWGG	3 cut(s)	559, 849, 1309
EcoRV	GATATC	1 cut(s)	327
EcoT38I	GRGCYC	1 cut(s)	532
Esp3I	CGTCTC	2 cut(s)	107, 1143
FaeI	CATG	5 cut(s)	282, 740, 1030, 1132, 1137
FaqI	GGGAC	1 cut(s)	400
FatI	CATG	5 cut(s)	278, 736, 1026, 1128, 1133
FauI	CCCGC	2 cut(s)	50, 468
FauNDI	CATATG	1 cut(s)	502
Fnu4HI	GCNGC	4 cut(s)	183, 315, 473, 996
FokI	GGATG	2 cut(s)	55, 334
FriOI	GRGCYC	1 cut(s)	532
Fsp4HI	GCNGC	4 cut(s)	183, 315, 473, 996
FspBI	CTAG	3 cut(s)	350, 779, 1232
GluI	GCNGC	4 cut(s)	183, 315, 473, 996
GsaI	CCCAGC	2 cut(s)	64, 321
GsuI	CTGGAG	2 cut(s)	162, 171
HaeIII	GGCC	2 cut(s)	115, 373
HapII	CCGG	2 cut(s)	857, 1263
HgaI	GACGC	3 cut(s)	342, 784, 1237
Hin1I	GRCGYC	1 cut(s)	795
Hin1II	CATG	5 cut(s)	282, 740, 1030, 1132, 1137
HinfI	GANTC	2 cut(s)	141, 455
HpaII	CCGG	2 cut(s)	857, 1263
HphI	GGTGA	3 cut(s)	235, 493, 1247
Hpy166II	GTNNAC	2 cut(s)	936, 1081
Hpy188I	TCNGA	2 cut(s)	409, 591
Hpy188III	TCNNGA	4 cut(s)	138, 188, 428, 949
Hpy8I	GTNNAC	2 cut(s)	936, 1081
Hpy99I	CGWCG	2 cut(s)	611, 1253
HpyAV	CCTTC	3 cut(s)	36, 425, 1165
HpyCH4III	ACNGT	1 cut(s)	1103
HpyCH4IV	ACGT	2 cut(s)	643, 819
HpyF10VI	GCNNNNNNNGC	7 cut(s)	182, 237, 527, 629, 788, 1004, 1134
HpyF3I	CTNAG	5 cut(s)	12, 307, 1053, 1224, 1236
HpySE526I	ACGT	2 cut(s)	643, 819
Hsp92I	GRCGYC	1 cut(s)	795
Hsp92II	CATG	5 cut(s)	282, 740, 1030, 1132, 1137
Kzo9I	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
LguI	GCTCTTC	1 cut(s)	203
LmnI	GCTCC	4 cut(s)	181, 190, 542, 715
Lsp1109I	GCAGC	4 cut(s)	169, 326, 459, 982
LweI	GCATC	5 cut(s)	508, 647, 1007, 1012, 1235
MaeI	CTAG	3 cut(s)	350, 779, 1232
MaeII	ACGT	2 cut(s)	643, 819
MaeIII	GTNAC	4 cut(s)	677, 820, 1039, 1227
MalI	GATC	7 cut(s)	252, 406, 498, 588, 912, 988, 1141
MboI	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
MfeI	CAATTG	1 cut(s)	1014
MflI	RGATCY	3 cut(s)	404, 586, 1139
MhlI	GDGCHC	1 cut(s)	532
MluCI	AATT	8 cut(s)	133, 287, 291, 446, 730, 876, 1014, 1181
MmeI	TCCRAC	2 cut(s)	564, 646
MseI	TTAA	7 cut(s)	290, 294, 345, 536, 627, 866, 879
MslI	CAYNNNNRTG	1 cut(s)	851
MspA1I	CMGCKG	3 cut(s)	182, 317, 995
MspI	CCGG	2 cut(s)	857, 1263
MspR9I	CCNGG	4 cut(s)	561, 851, 1264, 1311
MunI	CAATTG	1 cut(s)	1014
MvaI	CCWGG	3 cut(s)	561, 851, 1311
MwoI	GCNNNNNNNGC	7 cut(s)	182, 237, 527, 629, 788, 1004, 1134
NciI	CCSGG	1 cut(s)	1264
NdeI	CATATG	1 cut(s)	502
NdeII	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
NheI	GCTAGC	1 cut(s)	778
NlaIII	CATG	5 cut(s)	282, 740, 1030, 1132, 1137
NlaIV	GGNNCC	3 cut(s)	116, 416, 855
NmuCI	GTSAC	1 cut(s)	820
NspI	RCATGY	1 cut(s)	1132
OliI	CACNNNNGTG	1 cut(s)	851
PacI	TTAATTAA	1 cut(s)	294
PaeI	GCATGC	1 cut(s)	1132
PaeR7I	CTCGAG	1 cut(s)	552
PciSI	GCTCTTC	1 cut(s)	203
PfeI	GAWTC	2 cut(s)	141, 455
PfoI	TCCNGGA	2 cut(s)	559, 1309
PkrI	GCNGC	4 cut(s)	184, 316, 474, 997
Ppu21I	YACGTR	1 cut(s)	644
PpuMI	RGGWCCY	2 cut(s)	414, 557
PshBI	ATTAAT	3 cut(s)	290, 294, 879
PsiI	TTATAA	1 cut(s)	971
Psp5II	RGGWCCY	2 cut(s)	414, 557
Psp6I	CCWGG	3 cut(s)	559, 849, 1309
PspFI	CCCAGC	2 cut(s)	60, 317
PspGI	CCWGG	3 cut(s)	559, 849, 1309
PspN4I	GGNNCC	3 cut(s)	116, 416, 855
PspPI	GGNCC	4 cut(s)	114, 371, 414, 557
PspPPI	RGGWCCY	2 cut(s)	414, 557
PspXI	VCTCGAGB	1 cut(s)	552
PstI	CTGCAG	1 cut(s)	1078
PstNI	CAGNNNCTG	2 cut(s)	11, 144
PsuI	RGATCY	3 cut(s)	404, 586, 1139
PvuII	CAGCTG	3 cut(s)	182, 317, 995
RseI	CAYNNNNRTG	1 cut(s)	851
SapI	GCTCTTC	1 cut(s)	203
SaqAI	TTAA	7 cut(s)	290, 294, 345, 536, 627, 866, 879
SatI	GCNGC	4 cut(s)	183, 315, 473, 996
Sau3AI	GATC	7 cut(s)	250, 404, 496, 586, 910, 986, 1139
Sau96I	GGNCC	4 cut(s)	114, 371, 414, 557
ScrFI	CCNGG	4 cut(s)	561, 851, 1264, 1311
SduI	GDGCHC	1 cut(s)	532
SfaNI	GCATC	5 cut(s)	508, 647, 1007, 1012, 1235
SfcI	CTRYAG	1 cut(s)	1074
Sfr274I	CTCGAG	1 cut(s)	552
SinI	GGWCC	2 cut(s)	414, 557
SlaI	CTCGAG	1 cut(s)	552
SmiMI	CAYNNNNRTG	1 cut(s)	851
SmlI	CTYRAG	1 cut(s)	552
SmoI	CTYRAG	1 cut(s)	552
SphI	GCATGC	1 cut(s)	1132
Sse9I	AATT	8 cut(s)	133, 287, 291, 446, 730, 876, 1014, 1181
SsiI	CCGC	2 cut(s)	43, 475
SspI	AATATT	1 cut(s)	298
SspMI	CTAG	3 cut(s)	350, 779, 1232
StyD4I	CCNGG	4 cut(s)	559, 849, 1262, 1309
TaaI	ACNGT	1 cut(s)	1103
TaiI	ACGT	2 cut(s)	646, 822
TaqI	TCGA	5 cut(s)	77, 130, 553, 609, 753
TasI	AATT	8 cut(s)	133, 287, 291, 446, 730, 876, 1014, 1181
TfiI	GAWTC	2 cut(s)	141, 455
Tru1I	TTAA	7 cut(s)	290, 294, 345, 536, 627, 866, 879
Tru9I	TTAA	7 cut(s)	290, 294, 345, 536, 627, 866, 879
TseFI	GTSAC	1 cut(s)	820
TseI	GCWGC	4 cut(s)	182, 314, 472, 995
Tsp45I	GTSAC	1 cut(s)	820
TspDTI	ATGAA	6 cut(s)	18, 725, 771, 1043, 1059, 1176
TspGWI	ACGGA	1 cut(s)	456
VpaK11BI	GGWCC	2 cut(s)	414, 557
VspI	ATTAAT	3 cut(s)	290, 294, 879
XapI	RAATTY	2 cut(s)	133, 730
XceI	RCATGY	1 cut(s)	1132
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1033
XhoI	CTCGAG	1 cut(s)	552
XspI	CTAG	3 cut(s)	350, 779, 1232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.