Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008337

Physical Location & Seq

Genomic Coordinates Reverse (-)

6972738 .. 6977764

Gene Structure 5027 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1073300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGCAGTAGCTGAGCTATCAACACCTTCATACACCAAGACCCACCTTTTTCAATTATCCCAGCTCTCATCCTTATCGACCCATCTCCACTCTCACCGCCGTGTGGCTTTTCGGCCGCTCCCTCACACTCGAAATGCCAGAATCTGTTGCTCTGTTGCACCCAATCAAGTTCAAGCTCCAGCAGCTCCAGCGCAAACCGAAGACCCGAAGAACAAGCCTGAGTGCTTCGGCGTGTTCTGCCTCACCTATGATCTCCAAGCTGAAGAAGAGACAAAATCATGGAAGAAATTAATTAATATTGCAGTCTCAGGTGCAGCTGGGATGATATCTAATCATCTGCTCTTTAAACTTGCGTCAGGCGAGGTTTTTGGGCCAGACCAACCTATTGCGTTGAAACTATTGGGATCTGAAAGGTCCTTCCAAGCTCTTGAAGTGGCTATGGAATTGGAGGATTCTCTATTTCCGTTGTTGCGGGAGGTGAGCATTGGCATTGATCCATATGAGGTTTTCCAAGATGTAGAATGGGCTCTTTTAATAGGAGCAAAGCCTCGAGGTCCTGGAATGGAAAGAGCAGACTTGTTGGATCTGAATGGGCAGATTTTTGTTGAGCAGGGAAAAGCACTTAATGCTGTTGCATCACATAATGTCAAAGTGATAGTAGTTGGAAACCCTTGTAACACAAATGCACTCATCTGTTTGAAAAATGCTCCAAACATACCTGCAAAGAATTTTCATGCTTTAACACGGCTGGATGAAAATAGAGCAAAATGTCAGCTAGCCCTCAAAGCAGGTGTCTTCTATGATAAAGTGTCAAACGTGACCATCTGGGGAAACCACTCAACCACCCAGGTGCCAGACTTCTTAAATGCTAGAATTAATGGCTTGCCGGTCAAAGAGGTCATCAAGGATCACAAATGGTTAGAGGAAGAATTTACCGAACTTATCCAGAAGAGAGGAGGTGTGCTCATCAAGAAATGGGGAAGATCGTCAGCTGCATCAACTGCCATGTCAATTGCAGATGCCATAAAGTCTTTGATTACTCCTACCCCAGAGGGCGATTGGTTTTCATCTGCAGTTTACACAAATGGAAATCCCTATGGTATAGCAGAGGATTTAGTATTCAGTATGCCATGCAGATCTAAAGGAGACGGCGACTATGAACTTGTAAAGGACATACAATTTGATGACTATCTTCGCAAGCGAATAAAAAAGAGCGAAGCTGAGTTACTAGCTGAGAAAAGATGTGTCGCTCACCTTACTGGGCAGGGTATCGCTGTGTGTGATTTACCCGAGGATACTATGCTTCCTGGAGAGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006107 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008746 GO:0009060 GO:0009117 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0016651 GO:0016652 GO:0016999 GO:0017144 GO:0019362 GO:0019637 GO:0019674 GO:0019752 GO:0030060 GO:0031967 GO:0031975 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046483 GO:0046496 GO:0048046 GO:0050896 GO:0051186 GO:0051775 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072524 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

440

Amino Acids

48.32

Weight (kDa)

6.16

Isoelectric Point (pI)

32.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	99 - 245	3.3e-32	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	248 - 418	3.5e-48	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58330 AT5G58330 AT5G58330
fragaria_vesca	FvH4_7g06530
malus_domestica	MD02G1243100.v1.1 MD07G1073300.v1.1
prunus_persica	Prupe.2G091600_v2.0.a1
pyrus_communis	pycom02g20970 pycom07g05680
rosa_chinensis	RchiOBHm_Chr1g0334961
rosa_laevigata	RLG00000029409
rosa_multiflora	Rmu_co7973608.1_g000002 Rmu_sc0000574.1_g000062 Rmu_sc0000574.1_g000065
rosa_roxburghii	Rroxscaffold_4G00316350
rosa_rugosa	Rorug01G0118000 Rorug01G0118100
rosa_samantha	Rh1AG141400 Rh1BG108900 Rh1CG133300 Rh1DG145900 Rh1DG146000
rosa_wichuraiana	Rw1G011730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	779
AbsI	CCTCGAGG	1 cut(s)	549
Acc36I	ACCTGC	2 cut(s)	727, 779
AccB1I	GGYRCC	1 cut(s)	850
AccBSI	CCGCTC	1 cut(s)	118
AciI	CCGC	3 cut(s)	97, 116, 472
AclWI	GGATC	4 cut(s)	412, 488, 591, 915
AcoI	YGGCCR	1 cut(s)	113
AcsI	RAATTY	2 cut(s)	727, 929
AcuI	CTGAAG	1 cut(s)	282
AfiI	CCNNNNNNNGG	1 cut(s)	103
AgsI	TTSAA	5 cut(s)	53, 173, 394, 431, 700
AjnI	CCWGG	3 cut(s)	556, 846, 1306
AleI	CACNNNNGTG	2 cut(s)	99, 848
Alw21I	GWGCWC	1 cut(s)	966
Alw26I	GTCTC	3 cut(s)	263, 310, 1140
AlwI	GGATC	4 cut(s)	412, 488, 591, 915
AlwNI	CAGNNNCTG	2 cut(s)	11, 144
Ama87I	CYCGRG	2 cut(s)	549, 1289
AoxI	GGCC	2 cut(s)	113, 371
ApeKI	GCWGC	3 cut(s)	182, 314, 992
ApoI	RAATTY	2 cut(s)	727, 929
ArsI	GACNNNNNNTTYG	4 cut(s)	777, 809, 1163, 1195
AseI	ATTAAT	3 cut(s)	290, 294, 876
AspLEI	GCGC	1 cut(s)	193
AspS9I	GGNCC	3 cut(s)	371, 414, 554
AsuHPI	GGTGA	4 cut(s)	86, 235, 490, 1244
AsuNHI	GCTAGC	1 cut(s)	775
AvaI	CYCGRG	2 cut(s)	549, 1289
AvaII	GGWCC	2 cut(s)	414, 554
BanI	GGYRCC	1 cut(s)	850
BanII	GRGCYC	1 cut(s)	529
BarI	GAAGNNNNNNTAC	2 cut(s)	1209, 1241
BbsI	GAAGAC	2 cut(s)	207, 787
Bbv12I	GWGCWC	1 cut(s)	966
BbvI	GCAGC	3 cut(s)	194, 326, 979
BccI	CCATC	2 cut(s)	90, 830
BceAI	ACGGC	3 cut(s)	84, 761, 1165
BciT130I	CCWGG	3 cut(s)	558, 848, 1308
BciVI	GTATCC	1 cut(s)	1288
BcoDI	GTCTC	3 cut(s)	263, 310, 1140
BfaI	CTAG	3 cut(s)	776, 870, 1229
BfmI	CTRYAG	1 cut(s)	1071
BfuAI	ACCTGC	2 cut(s)	727, 779
BfuI	GTATCC	1 cut(s)	1288
BglII	AGATCT	1 cut(s)	1136
BisI	GCNGC	4 cut(s)	116, 183, 315, 993
BlpI	GCTNAGC	1 cut(s)	12
BlsI	GCNGC	4 cut(s)	117, 184, 316, 994
Bme1390I	CCNGG	3 cut(s)	558, 848, 1308
Bme18I	GGWCC	2 cut(s)	414, 554
BmeT110I	CYCGRG	2 cut(s)	549, 1289
BmgT120I	GGNCC	3 cut(s)	371, 414, 554
BmiI	GGNNCC	1 cut(s)	852
BmrFI	CCNGG	3 cut(s)	558, 848, 1308
BmrI	ACTGGG	1 cut(s)	1269
BmsI	GCATC	3 cut(s)	644, 1004, 1009
BmtI	GCTAGC	1 cut(s)	779
BmuI	ACTGGG	1 cut(s)	1269
BpiI	GAAGAC	2 cut(s)	207, 787
BplI	GAGNNNNNCTC	2 cut(s)	948, 980
BpmI	CTGGAG	2 cut(s)	162, 171
Bpu1102I	GCTNAGC	1 cut(s)	12
BsaBI	GATNNNNATC	1 cut(s)	1188
BsaJI	CCNNGG	2 cut(s)	846, 1290
Bsc4I	CCNNNNNNNGG	1 cut(s)	103
Bse118I	RCCGGY	1 cut(s)	886
Bse1I	ACTGG	1 cut(s)	1264
Bse8I	GATNNNNATC	1 cut(s)	1188
BseBI	CCWGG	3 cut(s)	558, 848, 1308
BseDI	CCNNGG	2 cut(s)	846, 1290
BseGI	GGATG	3 cut(s)	68, 327, 757
BseJI	GATNNNNATC	1 cut(s)	1188
BseLI	CCNNNNNNNGG	1 cut(s)	103
BseMII	CTCAG	4 cut(s)	210, 321, 1212, 1224
BseNI	ACTGG	1 cut(s)	1264
BseRI	GAGGAG	1 cut(s)	969
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	3 cut(s)	194, 326, 979
BseYI	CCCAGC	2 cut(s)	60, 317
BsgI	GTGCAG	1 cut(s)	333
Bsh1285I	CGRYCG	1 cut(s)	116
BshFI	GGCC	2 cut(s)	115, 373
BshNI	GGYRCC	1 cut(s)	850
BsiEI	CGRYCG	1 cut(s)	116
BsiHKAI	GWGCWC	1 cut(s)	966
BsiHKCI	CYCGRG	2 cut(s)	549, 1289
BsiSI	CCGG	1 cut(s)	887
BslI	CCNNNNNNNGG	1 cut(s)	103
BsmAI	GTCTC	3 cut(s)	263, 310, 1140
BsmBI	CGTCTC	1 cut(s)	1140
BsnI	GGCC	2 cut(s)	115, 373
BsoBI	CYCGRG	2 cut(s)	549, 1289
Bsp1286I	GDGCHC	2 cut(s)	529, 966
Bsp143I	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
Bsp1720I	GCTNAGC	1 cut(s)	12
BspACI	CCGC	3 cut(s)	97, 116, 472
BspANI	GGCC	2 cut(s)	115, 373
BspCNI	CTCAG	5 cut(s)	4, 211, 320, 1213, 1225
BspLI	GGNNCC	1 cut(s)	852
BspMAI	CTGCAG	1 cut(s)	1075
BspMI	ACCTGC	2 cut(s)	727, 779
BspOI	GCTAGC	1 cut(s)	779
BspPI	GGATC	4 cut(s)	412, 488, 591, 915
BspT107I	GGYRCC	1 cut(s)	850
BsrBI	CCGCTC	1 cut(s)	118
BsrFI	RCCGGY	1 cut(s)	886
BsrI	ACTGG	1 cut(s)	1264
BssAI	RCCGGY	1 cut(s)	886
BssECI	CCNNGG	2 cut(s)	846, 1290
BssMI	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
Bst2UI	CCWGG	3 cut(s)	558, 848, 1308
Bst6I	CTCTTC	2 cut(s)	261, 944
BstAPI	GCANNNNNTGC	2 cut(s)	626, 1001
BstC8I	GCNNGC	3 cut(s)	777, 884, 1199
BstDEI	CTNAG	5 cut(s)	12, 219, 307, 1221, 1233
BstF5I	GGATG	3 cut(s)	68, 327, 757
BstHHI	GCGC	1 cut(s)	193
BstKTI	GATC	7 cut(s)	253, 407, 496, 586, 910, 986, 1139
BstMAI	GTCTC	3 cut(s)	263, 310, 1140
BstMBI	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
BstMCI	CGRYCG	1 cut(s)	116
BstMWI	GCNNNNNNNGC	6 cut(s)	182, 188, 237, 626, 785, 1001
BstNI	CCWGG	3 cut(s)	558, 848, 1308
BstSCI	CCNGG	3 cut(s)	556, 846, 1306
BstSFI	CTRYAG	1 cut(s)	1071
BstV1I	GCAGC	3 cut(s)	194, 326, 979
BstV2I	GAAGAC	2 cut(s)	207, 787
BstX2I	RGATCY	3 cut(s)	404, 583, 1136
BstYI	RGATCY	3 cut(s)	404, 583, 1136
BstZI	CGGCCG	1 cut(s)	113
BsuI	GTATCC	1 cut(s)	1288
BsuRI	GGCC	2 cut(s)	115, 373
BtsCI	GGATG	3 cut(s)	68, 327, 757
BveI	ACCTGC	2 cut(s)	727, 779
Cac8I	GCNNGC	3 cut(s)	777, 884, 1199
CaiI	CAGNNNCTG	2 cut(s)	11, 144
CfoI	GCGC	1 cut(s)	193
Cfr10I	RCCGGY	1 cut(s)	886
Cfr13I	GGNCC	3 cut(s)	371, 414, 554
CseI	GACGC	1 cut(s)	342
CviAII	CATG	4 cut(s)	279, 734, 1006, 1131
DdeI	CTNAG	5 cut(s)	12, 219, 307, 1221, 1233
DpnI	GATC	7 cut(s)	252, 406, 495, 585, 909, 985, 1138
DpnII	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
DraI	TTTAAA	1 cut(s)	346
EaeI	YGGCCR	1 cut(s)	113
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	2 cut(s)	261, 944
EarI	CTCTTC	2 cut(s)	261, 944
EclXI	CGGCCG	1 cut(s)	113
Eco24I	GRGCYC	1 cut(s)	529
Eco32I	GATATC	1 cut(s)	327
Eco47I	GGWCC	2 cut(s)	414, 554
Eco52I	CGGCCG	1 cut(s)	113
Eco57I	CTGAAG	1 cut(s)	282
Eco88I	CYCGRG	2 cut(s)	549, 1289
EcoO109I	RGGNCCY	2 cut(s)	414, 554
EcoRII	CCWGG	3 cut(s)	556, 846, 1306
EcoRV	GATATC	1 cut(s)	327
EcoT38I	GRGCYC	1 cut(s)	529
Esp3I	CGTCTC	1 cut(s)	1140
FaeI	CATG	4 cut(s)	282, 737, 1009, 1134
FatI	CATG	4 cut(s)	278, 733, 1005, 1130
FauI	CCCGC	1 cut(s)	465
FauNDI	CATATG	1 cut(s)	499
Fnu4HI	GCNGC	4 cut(s)	116, 183, 315, 993
FokI	GGATG	3 cut(s)	55, 334, 764
FriOI	GRGCYC	1 cut(s)	529
Fsp4HI	GCNGC	4 cut(s)	116, 183, 315, 993
FspBI	CTAG	3 cut(s)	776, 870, 1229
GlaI	GCGC	1 cut(s)	192
GluI	GCNGC	4 cut(s)	116, 183, 315, 993
GsaI	CCCAGC	2 cut(s)	64, 321
GsuI	CTGGAG	2 cut(s)	162, 171
HaeIII	GGCC	2 cut(s)	115, 373
HapII	CCGG	1 cut(s)	887
HgaI	GACGC	1 cut(s)	342
HhaI	GCGC	1 cut(s)	193
Hin1II	CATG	4 cut(s)	282, 737, 1009, 1134
Hin6I	GCGC	1 cut(s)	191
HinP1I	GCGC	1 cut(s)	191
HinfI	GANTC	2 cut(s)	141, 452
HpaII	CCGG	1 cut(s)	887
HphI	GGTGA	4 cut(s)	86, 235, 490, 1244
Hpy166II	GTNNAC	1 cut(s)	1078
Hpy188I	TCNGA	2 cut(s)	409, 588
Hpy188III	TCNNGA	3 cut(s)	428, 946, 970
Hpy8I	GTNNAC	1 cut(s)	1078
HpyAV	CCTTC	2 cut(s)	36, 427
HpyCH4IV	ACGT	1 cut(s)	816
HpyF10VI	GCNNNNNNNGC	6 cut(s)	182, 188, 237, 626, 785, 1001
HpyF3I	CTNAG	5 cut(s)	12, 219, 307, 1221, 1233
HpySE526I	ACGT	1 cut(s)	816
Hsp92II	CATG	4 cut(s)	282, 737, 1009, 1134
HspAI	GCGC	1 cut(s)	191
Kzo9I	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
LmnI	GCTCC	5 cut(s)	123, 181, 190, 539, 712
Lsp1109I	GCAGC	3 cut(s)	194, 326, 979
LweI	GCATC	3 cut(s)	644, 1004, 1009
MaeI	CTAG	3 cut(s)	776, 870, 1229
MaeII	ACGT	1 cut(s)	816
MaeIII	GTNAC	3 cut(s)	674, 817, 1224
MalI	GATC	7 cut(s)	252, 406, 495, 585, 909, 985, 1138
MbiI	CCGCTC	1 cut(s)	118
MboI	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
MfeI	CAATTG	1 cut(s)	1011
MflI	RGATCY	3 cut(s)	404, 583, 1136
MhlI	GDGCHC	2 cut(s)	529, 966
MluCI	AATT	9 cut(s)	53, 287, 291, 443, 727, 873, 929, 1011, 1178
MmeI	TCCRAC	2 cut(s)	561, 643
MseI	TTAA	8 cut(s)	290, 294, 345, 533, 624, 740, 863, 876
MslI	CAYNNNNRTG	2 cut(s)	99, 848
MspA1I	CMGCKG	2 cut(s)	317, 992
MspI	CCGG	1 cut(s)	887
MspR9I	CCNGG	3 cut(s)	558, 848, 1308
MunI	CAATTG	1 cut(s)	1011
MvaI	CCWGG	3 cut(s)	558, 848, 1308
MwoI	GCNNNNNNNGC	6 cut(s)	182, 188, 237, 626, 785, 1001
NdeI	CATATG	1 cut(s)	499
NdeII	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
NheI	GCTAGC	1 cut(s)	775
NlaIII	CATG	4 cut(s)	282, 737, 1009, 1134
NlaIV	GGNNCC	1 cut(s)	852
NmuCI	GTSAC	1 cut(s)	817
OliI	CACNNNNGTG	2 cut(s)	99, 848
PacI	TTAATTAA	1 cut(s)	294
PaeR7I	CTCGAG	1 cut(s)	549
PaqCI	CACCTGC	1 cut(s)	779
PfeI	GAWTC	2 cut(s)	141, 452
PfoI	TCCNGGA	2 cut(s)	556, 1306
PkrI	GCNGC	4 cut(s)	117, 184, 316, 994
PpuMI	RGGWCCY	2 cut(s)	414, 554
PshBI	ATTAAT	3 cut(s)	290, 294, 876
Psp5II	RGGWCCY	2 cut(s)	414, 554
Psp6I	CCWGG	3 cut(s)	556, 846, 1306
PspFI	CCCAGC	2 cut(s)	60, 317
PspGI	CCWGG	3 cut(s)	556, 846, 1306
PspN4I	GGNNCC	1 cut(s)	852
PspPI	GGNCC	3 cut(s)	371, 414, 554
PspPPI	RGGWCCY	2 cut(s)	414, 554
PspXI	VCTCGAGB	1 cut(s)	549
PstI	CTGCAG	1 cut(s)	1075
PstNI	CAGNNNCTG	2 cut(s)	11, 144
PsuI	RGATCY	3 cut(s)	404, 583, 1136
PvuII	CAGCTG	2 cut(s)	317, 992
RseI	CAYNNNNRTG	2 cut(s)	99, 848
SaqAI	TTAA	8 cut(s)	290, 294, 345, 533, 624, 740, 863, 876
SatI	GCNGC	4 cut(s)	116, 183, 315, 993
Sau3AI	GATC	7 cut(s)	250, 404, 493, 583, 907, 983, 1136
Sau96I	GGNCC	3 cut(s)	371, 414, 554
ScrFI	CCNGG	3 cut(s)	558, 848, 1308
SduI	GDGCHC	2 cut(s)	529, 966
SfaNI	GCATC	3 cut(s)	644, 1004, 1009
SfcI	CTRYAG	1 cut(s)	1071
Sfr274I	CTCGAG	1 cut(s)	549
SinI	GGWCC	2 cut(s)	414, 554
SlaI	CTCGAG	1 cut(s)	549
SmiMI	CAYNNNNRTG	2 cut(s)	99, 848
SmlI	CTYRAG	1 cut(s)	549
SmoI	CTYRAG	1 cut(s)	549
Sse9I	AATT	9 cut(s)	53, 287, 291, 443, 727, 873, 929, 1011, 1178
SsiI	CCGC	3 cut(s)	97, 116, 472
SspI	AATATT	1 cut(s)	298
SspMI	CTAG	3 cut(s)	776, 870, 1229
StyD4I	CCNGG	3 cut(s)	556, 846, 1306
TaiI	ACGT	1 cut(s)	819
TaqI	TCGA	3 cut(s)	77, 130, 550
TasI	AATT	9 cut(s)	53, 287, 291, 443, 727, 873, 929, 1011, 1178
TauI	GCSGC	1 cut(s)	118
TfiI	GAWTC	2 cut(s)	141, 452
Tru1I	TTAA	8 cut(s)	290, 294, 345, 533, 624, 740, 863, 876
Tru9I	TTAA	8 cut(s)	290, 294, 345, 533, 624, 740, 863, 876
TseFI	GTSAC	1 cut(s)	817
TseI	GCWGC	3 cut(s)	182, 314, 992
Tsp45I	GTSAC	1 cut(s)	817
TspDTI	ATGAA	5 cut(s)	18, 722, 768, 1056, 1173
TspGWI	ACGGA	1 cut(s)	453
VpaK11BI	GGWCC	2 cut(s)	414, 554
VspI	ATTAAT	3 cut(s)	290, 294, 876
XapI	RAATTY	2 cut(s)	727, 929
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1056
XhoI	CTCGAG	1 cut(s)	549
XspI	CTAG	3 cut(s)	776, 870, 1229

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.